KR20230081961A - Food composition comprising fermented rice germ lactic acid bacteria - Google Patents

Food composition comprising fermented rice germ lactic acid bacteria Download PDF

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KR20230081961A
KR20230081961A KR1020220141282A KR20220141282A KR20230081961A KR 20230081961 A KR20230081961 A KR 20230081961A KR 1020220141282 A KR1020220141282 A KR 1020220141282A KR 20220141282 A KR20220141282 A KR 20220141282A KR 20230081961 A KR20230081961 A KR 20230081961A
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powder
weight
lactic acid
acid bacteria
food
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석보영
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/185Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01Instant products; Powders; Flakes; Granules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385Concentrates of non-alcoholic beverages
    • A23L2/39Dry compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L25/00Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
    • A23L25/30Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/104Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/115Cereal fibre products, e.g. bran, husk
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/324Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/02Acid
    • A23V2250/038Gamma-amino butyric acid
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/24Heat, thermal treatment
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/143Fermentum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/535Pseudocatenulatum

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Abstract

The present invention relates to powdered food for beverage production comprising lactic acid bacteria-fermented rice germ and a method for manufacturing the same. The powdered food comprising lactic acid bacteria-fermented rice germ and vegetable protein according to the present invention has excellent organoleptic properties such as aroma, taste, and swallowing sensation, to allow anyone to easily consume protein, and contains nutritional components of rice germ such as GABA, to be used in manufacturing convenient food or beverages with enhanced health functionality that are effective in improving immunity.

Description

쌀눈 유산균 발효물을 포함하는 식품 조성물{Food composition comprising fermented rice germ lactic acid bacteria}Food composition comprising fermented rice germ lactic acid bacteria}

본 발명은 유산균 발효 쌀눈을 포함하는 음료제조용 분말식품 및 이의 제조방법에 관한 것이다.The present invention relates to a powdered food for preparing a beverage containing lactic acid bacteria fermented rice bran and a manufacturing method thereof.

충분한 단백질의 섭취는 현대인에게 중요한 문제이다. 단백질은 근육의 재료일 뿐만 아니라 면역 세포를 구성하는 주성분이기 때문이다. 특히, 단백질은 각종 질환에 노출되기 쉬운 현대인에게 반드시 필요한 영양소이다. 식물을 기반으로 하는 식단에서 중요한 요소는 식물성 단백질로서, 건강을 위해서 식물성 단백질의 섭취는 중요한 문제이다. 간편함이 중요시되고 있는 현대사회에서, 식물성 단백질 섭취를 위하여 식물성 단백질 음료가 최근 각광받고 있다.Sufficient protein intake is an important problem for modern people. This is because protein is not only the material of muscle, but also the main component of immune cells. In particular, protein is an essential nutrient for modern people who are prone to various diseases. An important component of a plant-based diet is plant protein, and intake of plant protein is an important issue for health. In modern society where convenience is valued, vegetable protein beverages have recently been in the limelight for vegetable protein intake.

한편, 쌀을 주식으로 하는 국내에서 벼 생산량이 최근 약 552만톤 정도이며 도정시 발생하는 부산물인 왕겨는 약 118 만톤, 미강은 약 47만톤, 쌀눈은 약 20만톤 내외로 발생되는 것으로 추정된다. 특히 쌀눈은 다양한 영양적 가치 가 있는데도 불구하고 대부분이 미강에 혼입되어 사료로 사용되고 있다. 쌀 구조 중 2%를 차지하는 쌀눈은 배유와 비교하였을 때 양질의 단백질과 20% 이상의 지방, 무기질 및 비타민 A, B, E 등이 풍부할 뿐 아니라 항산화, 혈압강하, 뇌 혈류개선 등의 효능이 있는 가바 성분 등이 함유되어 있다. 이런 영양적 가치 때문에 쌀눈 기름, 쌀눈 비누, 숙취 음료 등의 제품이 출시되었으나 쌀겨로부터 쌀눈의 순수 분리가 어렵고 도정 후 lipase, lipoxydase의 작용으로 산패가 급격히 일어나, 쌀 눈 고유의 곡물취로 인하여 쌀눈을 소비자들이 직접 섭취하기에는 어려움이 있었다.On the other hand, rice production in Korea, where rice is the staple food, is currently about 5.52 million tons, and it is estimated that about 1.18 million tons of rice husk, rice bran, and about 200,000 tons of rice bran, which are by-products generated during milling, are generated. In particular, although rice bran has various nutritional values, most of it is mixed with rice bran and used as feed. Compared to the endosperm, the rice germ, which accounts for 2% of the rice structure, is rich in high-quality protein, more than 20% fat, minerals, and vitamins A, B, and E, as well as antioxidant, lowering blood pressure, and improving cerebral blood flow. It contains GABA ingredients and the like. Because of this nutritional value, products such as rice bran oil, rice bran soap, and hangover drinks have been released, but it is difficult to separate pure bran from rice bran, and rancidity occurs rapidly due to the action of lipase and lipoxydase after milling. It was difficult for them to consume directly.

이러한 쌀눈의 영양학적 효능을 극대화하는 쌀눈 유산균 발효물 및 식물성 단백질을 첨가한 조성물을 이용하여 누구나 섭취가 용이한 단백질 음료를 섭취할 수 있도록 개발하였다.It was developed so that anyone can consume a protein drink that is easy to consume by using a composition to which lactic acid bacteria fermented rice buds and vegetable proteins are added to maximize the nutritional efficacy of the rice buds.

본 발명은 유산균 발효 쌀눈, 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 포함하는 가바 함량이 향상된 음료제조용 분말식품 및 이의 제조방법을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a powdered food for preparing a beverage with improved GABA content, including lactic acid bacteria fermented rice bran, pea protein, oatmeal powder, soymilk powder, green tea powder and sugar, and a manufacturing method thereof.

본 발명의 분말식품은 고구마 분말 및 단호박 분말을 더 포함하거나 검정깨 분말 및 볶은흑미 분말을 더 포함하는 가바 함량이 향상된 음료제조용 분말식품을 제공하는 것을 목적으로 한다.An object of the powdered food of the present invention is to provide a powdered food for preparing a beverage with improved GABA content, further comprising sweet potato powder and sweet pumpkin powder, or further comprising black sesame powder and roasted black rice powder.

본 발명은 유산균 발효 쌀눈, 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 포함하는 가바 함량이 향상된 음료제조용 분말식품을 제공한다.The present invention provides a powdered food for preparing a beverage with improved GABA content, including lactic acid bacteria fermented rice bran, pea protein, oatmeal powder, soymilk powder, green tea powder and sugar.

상기 유산균 발효 쌀눈은 0.3 ~ 1 중량부, 완두콩 단백질은 20 ~ 35 중량부, 오트밀 분말은 15 ~ 30 중량부, 두유 분말은 3 ~ 15 중량부, 녹차 분말은 0.1 ~ 1 중량부, 설탕은 20 ~ 35 중량부로 포함될 수 있다.0.3 to 1 part by weight of the lactic acid bacteria fermented rice buds, 20 to 35 parts by weight of pea protein, 15 to 30 parts by weight of oatmeal powder, 3 to 15 parts by weight of soy milk powder, 0.1 to 1 part by weight of green tea powder, and 20 parts by weight of sugar ~ 35 parts by weight.

상기 조성물은 고구마 분말 및 단호박 분말을 더 포함할 수 있다.The composition may further include sweet potato powder and sweet pumpkin powder.

상기 고구마 분말은 20 ~ 30 중량부, 단호박 분말은 3 ~ 10 중량부로 포함되는 것일 수 있다.The sweet potato powder may be included in 20 to 30 parts by weight, and the sweet pumpkin powder in 3 to 10 parts by weight.

상기 조성물은 검정깨 분말 및 볶은흑미 분말을 더 포함할 수 있다.The composition may further include black sesame powder and roasted black rice powder.

상기 검정깨 분말은 3 ~ 15 중량부, 볶은흑미 분말은 3 ~ 15 중량부로 포함되는 것일 수 있다.The black sesame powder may be included in 3 to 15 parts by weight and the roasted black rice powder in 3 to 15 parts by weight.

상기 유산균 발효 쌀눈은 가바를 100g 당 90 내지 110mg포함하는 것일 수 있다.The lactic acid bacteria fermented rice bran may contain 90 to 110 mg of GABA per 100 g.

상기 분말식품 100중량%에 대하여, 유산균 발효 쌀눈 0.5 중량%, 완두콩 단백질 25 중량%, 오트밀 분말 17 중량%, 두유 분말 5 중량%, 녹차 분말 0.3 중량%, 설탕 23 중량%, 고구마 분말 24.2 중량% 및 단호박 분말 5 중량%을 포함하는 것일 수 있다.Based on 100% by weight of the powdered food, 0.5% by weight of lactic acid bacteria fermented rice bran, 25% by weight of pea protein, 17% by weight of oatmeal powder, 5% by weight of soymilk powder, 0.3% by weight of green tea powder, 23% by weight of sugar, 24.2% by weight of sweet potato powder and 5% by weight of sweet pumpkin powder.

상기 분말식품 100중량%에 대하여, 유산균 발효 쌀눈 0.5 중량%, 완두콩 단백질 25 중량%, 오트밀 분말 17 중량%, 두유 분말 5 중량%, 녹차 분말 0.3 중량%, 설탕 23 중량%, 검정깨 분말 7중량% 및 볶은흑미 분말 7중량%을 포함하는 것일 수 있다.Based on 100% by weight of the powdered food, 0.5% by weight of lactic acid bacteria fermented rice bran, 25% by weight of pea protein, 17% by weight of oatmeal powder, 5% by weight of soy milk powder, 0.3% by weight of green tea powder, 23% by weight of sugar, 7% by weight of black sesame powder % and 7% by weight of roasted black rice powder.

또한 본 발명은 상기 분말식품을 포함하는 식품 조성물을 제공한다.In addition, the present invention provides a food composition comprising the powdered food.

또한 본 발명은 1) 쌀눈에 혼합유산균을 접종하고 발효시켜 유산균 발효 쌀눈을 제조하는 단계; 및 2) 상기 유산균 발효 쌀눈에 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 배합하는 단계; 를 포함하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법을 제공한다.In addition, the present invention comprises the steps of 1) inoculating mixed lactic acid bacteria into rice buds and fermenting them to prepare lactic acid bacteria fermented rice buds; and 2) mixing pea protein, oatmeal powder, soymilk powder, green tea powder, and sugar with the lactic acid bacteria fermented rice buds; It provides a method for producing powdered food for beverage production with improved GABA content, comprising a.

상기 단계 1)의 쌀눈은 140 ~ 180℃에서 7 ~ 12분간 건열 처리된 것일 수 있다.The rice buds in step 1) may be dry heat treated at 140 to 180 ° C. for 7 to 12 minutes.

상기 단계 1)의 혼합유산균은 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 플란타룸(Lactobacillus plantarum), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 애시도필러스(Lactobacillus acidophilus), 락토바실러스 퍼멘텀(Lactobacillus fermentum), 비피도박테리움 롱검(Bifidobacterium longum) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)로 이루어진 군에서 선택되는 1종 이상을 혼합 배양한 것일 수 있다.The mixed lactic acid bacteria in step 1) are Lactobacillus casei , Lactobacillus plantarum , Lactobacillus rhamnosus , Lactobacillus acidophilus, Lactobacillus acidophilus , Lactobacillus fur Mentum ( Lactobacillus fermentum ), Bifidobacterium longum ( Bifidobacterium longum ) and Streptococcus thermophilus ( Streptococcus thermophilus ) It may be a mixed culture of one or more selected from the group consisting of.

상기 단계 1)의 발효는 혼합유산균 및 쌀눈을 1 : 1 ~ 2 의 중량 비율로 혼합 발효한 것일 수 있다.The fermentation in step 1) may be a mixed fermentation of mixed lactic acid bacteria and rice germ at a weight ratio of 1: 1 to 2.

상기 단계 2)의 배합은 45 내지 65 rpm에서 5 내지 15분간 수행되는 것일 수 있다.The mixing of step 2) may be performed at 45 to 65 rpm for 5 to 15 minutes.

상기 단계 2)에서 고구마 분말 및 단호박 분말을 더 포함하거나 또는 검정깨 분말 및 볶은흑미 분말을 더 포함할 수 있다.In step 2), sweet potato powder and sweet pumpkin powder may be further included, or black sesame powder and roasted black rice powder may be further included.

본 발명에 따른 유산균 발효 쌀눈 및 식물성 단백질을 함유하는 분말식품은 향, 맛, 목넘김 등의 관능품질이 우수하여 누구나 용이하게 단백질 섭취가 가능하고, 가바(GABA)와 같은 쌀눈의 영양학적 성분을 함유하여 면역력 증진에 효과적인 건강기능성이 강화된 간편음용 식품 또는 음료의 제조에 활용할 수 있다.Powdered food containing lactic acid bacteria fermented rice buds and vegetable protein according to the present invention has excellent sensory quality such as aroma, taste, and swallowing, so anyone can easily consume protein, and contains nutritional components of rice sprouts such as GABA Therefore, it can be used in the manufacture of food or beverages for easy drinking with strengthened health functionalities effective for enhancing immunity.

도 1은 유산균 발효 쌀눈의 제조공정 모식도를 나타낸 도이다.
도 2는 오트고구마 분말의 제조공정 모식도를 나타낸 도이다.
도 3은 오트흑임자 분말의 제조공정 모식도를 나타낸 도이다.
도 4는 오트고구마 분말 및 오트흑임자 분말의 이미지를 나타낸 도이다.
도 5는 고구마맛 음료(시료 A), 흑임자맛 음료(시료 B) 및 S사 단백질보충제(시료 C)의 관능품질평가를 나타낸 도이다.
1 is a diagram showing a schematic diagram of the manufacturing process of lactic acid bacteria fermented rice buds.
Figure 2 is a diagram showing a schematic diagram of the manufacturing process of oat sweet potato powder.
3 is a diagram showing a schematic diagram of the manufacturing process of oat black sesame powder.
4 is a diagram showing images of oat sweet potato powder and oat black sesame powder.
Figure 5 is a diagram showing the sensory quality evaluation of sweet potato-flavored beverages (sample A), black sesame-flavored beverages (sample B) and S company protein supplements (sample C).

본 발명자들은 식물성 단백질을 분말(파우더) 형태로 제공하여 물 또는 우유 등에 간편하게 타 먹으면서 단백질의 흡수율을 높이고, 다양한 영양학적 성분이 풍부한 유산균 발효 쌀눈을 함유하여 면역력 증진에 도움이 되는 음료제조용 분말식품을 제조하였다. The present inventors provided vegetable protein in the form of a powder (powder) to increase the absorption rate of protein while easily mixing it with water or milk, and containing lactic acid bacteria fermented rice buds rich in various nutritional ingredients to provide powdered food for beverage production that helps improve immunity. manufactured.

본 발명은 유산균 발효 쌀눈, 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 포함하는, 가바 함량이 향상된 음료제조용 분말식품에 관한 것이다.The present invention relates to a powdered food for preparing a beverage with improved GABA content, including lactic acid bacteria fermented rice bran, pea protein, oatmeal powder, soymilk powder, green tea powder and sugar.

본 발명은 가바 함량이 향상된 유산균 발효 쌀눈에 상기 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 등의 식물성 단백질을 생체 내에서 흡수율을 높이면서 용해성 및 관능품질이 우수한 최적의 배합비로 혼합하여 분말 형태로 제공할 수 있다.In the present invention, vegetable proteins such as pea protein, oatmeal powder, soy milk powder, green tea powder, etc. are mixed with lactic acid bacteria fermented rice sprouts with improved GABA content in an optimal blending ratio with excellent solubility and sensory quality while increasing the absorption rate in vivo to form a powder. can provide

본 발명의 분말식품은 상기 용해성, 관능품질이 우수한 최적의 배합비로 상기 유산균 발효 쌀눈은 0.3 ~ 1 중량부, 완두콩 단백질은 20 ~ 35 중량부, 오트밀 분말은 15 ~ 30 중량부, 두유 분말은 3 ~ 15 중량부, 녹차 분말은 0.1 ~ 1 중량부, 설탕은 20 ~ 35 중량부로 포함될 수 있다.The powdered food of the present invention is an optimal blending ratio with excellent solubility and sensory quality, 0.3 to 1 part by weight of the lactic acid bacteria fermented rice buds, 20 to 35 parts by weight of pea protein, 15 to 30 parts by weight of oatmeal powder, and 3 parts by weight of soymilk powder. ~ 15 parts by weight, 0.1 to 1 part by weight of green tea powder, and 20 to 35 parts by weight of sugar may be included.

본 발명의 분말식품은 단백질 흡수율, 소화율, 맛과 목넘김 등의 관능품질 뿐만 아니라 식사 대용의 영양학적 가치를 고려하여 고구마 분말 및 단호박 분말을 더 포함하거나, 검정깨 분말 및 볶은흑미 분말을 더 포함할 수 있다. 상기 고구마 분말을 20 ~ 30 중량부 및 단호박 분말을 3 ~ 10 중량부로 더 포함하여 오트고구마 분말을 제공하고, 상기 검정깨 분말을 3 ~ 15 중량부 및 볶은흑미 분말을 3 ~ 15 중량부로 더 포함하여 오트흑임자 분말을 제공할 수 있다.The powdered food of the present invention may further include sweet potato powder and sweet pumpkin powder, or black sesame powder and roasted black rice powder in consideration of sensory qualities such as protein absorption rate, digestibility, taste and swallowing, as well as nutritional value as a meal substitute. can Further comprising 20 to 30 parts by weight of the sweet potato powder and 3 to 10 parts by weight of sweet pumpkin powder to provide oat sweet potato powder, and further comprising 3 to 15 parts by weight of the black sesame powder and 3 to 15 parts by weight of roasted black rice powder Thus, oat black sesame powder can be provided.

본 발명의 일구현예에 따르면, 상기 오트고구마 분말 또는 오트흑임자 분말은 평균 1분 내지 3분 이내에 분말 덩어리가 잔존하는 현상 없이 물에서 완전히 용해되었으며, 이러한 우수한 수용해성은 섭취시 이물감을 부여하지 않는 목넘김이 우수한 관능을 나타냈다. According to one embodiment of the present invention, the oat sweet potato powder or oat black sesame powder was completely dissolved in water within an average of 1 to 3 minutes without the phenomenon of remaining powder lumps, and such excellent water solubility does not give a foreign body feeling when ingested. It showed excellent sensuality.

구체적으로 상기 오트고구마 분말을 포함하는 고구마맛 음료, 상기 오트흑임자 분말을 포함하는 흑임자맛 음료를 제조한 후 점도, 맛, 향, 목넘김에 대한 관능검사를 시행하고 S사의 단백질보충제 1종을 비교군으로 품질평가를 진행한 결과, 본 발명에 따른 고구마맛 음료 및 흑임자맛 음료 모두 비교군 대비 점도 및 목넘김에서 높은 점수를 반영하였다. 또한 고소한 맛과 단 맛이 적절히 어우러져 전체적인 기호성이 우수하게 나타나 기존의 단백질 혼합음료에서 흔히 발생하는 특유의 단백질 맛이 전혀 나지 않아 거부감 없이 음료를 섭취할 수 있는 효과가 있음을 확인하였다.Specifically, after preparing a sweet potato-flavored beverage containing the oat sweet potato powder and a black sesame-flavored beverage containing the oat black sesame powder, a sensory test for viscosity, taste, aroma, and swallowing was performed, and one protein supplement from S company was used as a comparison group. As a result of the quality evaluation, both the sweet potato-flavored beverage and the black sesame-flavored beverage according to the present invention reflected high scores in viscosity and throat swallowing compared to the comparative group. In addition, it was confirmed that the overall palatability was excellent because the savory taste and sweet taste were properly harmonized, so that the unique protein taste commonly found in existing protein mixed beverages was not present at all, so that the beverage could be consumed without objection.

본 발명의 분말식품은 오트고구마 분말의 경우 바람직하게는 분말 전체 100중량%에 대하여, 유산균 발효 쌀눈 0.5 중량%, 완두콩 단백질 25 중량%, 오트밀 분말 17 중량%, 두유 분말 5 중량%, 녹차 분말 0.3 중량%, 설탕 23 중량%, 고구마 분말 24.2 중량% 및 단호박 분말 5 중량%을 포함할 수 있다.In the case of oat sweet potato powder, the powdered food of the present invention preferably contains 0.5% by weight of lactic acid bacteria fermented rice buds, 25% by weight of pea protein, 17% by weight of oatmeal powder, 5% by weight of soy milk powder, and 0.3% by weight of green tea powder, based on 100% by weight of the total powder. % by weight, 23% by weight sugar, 24.2% by weight sweet potato powder, and 5% by weight sweet pumpkin powder.

오트흑임자 분말의 경우 바람직하게는 분말 전체 100중량%에 대하여, 유산균 발효 쌀눈 0.5 중량%, 완두콩 단백질 25 중량%, 오트밀 분말 17 중량%, 두유 분말 5 중량%, 녹차 분말 0.3 중량%, 설탕 23 중량%, 검정깨 분말 7중량% 및 볶음흑미 분말 7중량%을 포함할 수 있다.In the case of oat black sesame powder, preferably, 0.5% by weight of lactic acid bacteria fermented rice bran, 25% by weight of pea protein, 17% by weight of oatmeal powder, 5% by weight of soymilk powder, 0.3% by weight of green tea powder, 23% by weight of sugar, based on 100% by weight of the total powder. %, 7% by weight of black sesame powder and 7% by weight of roasted black rice powder.

본 발명의 분말식품은 유산균 발효 쌀눈을 함유함으로써 가바 함량이 증진된 것일 수 있으며, 상기 유산균 발효 쌀눈은 가바를 100g 당 90 내지 110mg 포함할 수 있다.The powdered food of the present invention may have an increased GABA content by containing lactic acid bacteria fermented rice buds, and the lactic acid bacteria fermented rice buds may contain 90 to 110 mg of GABA per 100 g.

가바(GABA, γ-aminobutyric acid)는 자연계에 널리 분포하는 비단백태 아미노산의 일종으로 흥분을 억제시키는 신경전달물질로서 뇌의 혈류를 개선하여 산소공급을 증가시켜 두뇌 식품(brain food)의 활용으로 각광받고 있는 물질이다. 가바는 두뇌의 브레이크처럼 작용하여 두뇌와 중추 신경계의 뉴런 활동을 감소시켜 수면을 촉진하고 정신적 육체적 스트레스를 줄이며 불안을 낮추고 스트레스 조건에서 면역성을 향상시키는 등의 다양한 효능이 있는 것으로 알려져 있다.GABA (γ-aminobutyric acid) is a kind of non-protein amino acid widely distributed in nature. As a neurotransmitter that suppresses excitement, it improves blood flow to the brain and increases oxygen supply, drawing attention as a brain food. material that is being received. GABA acts like a brake on the brain, reducing neuronal activity in the brain and central nervous system, promoting sleep, reducing mental and physical stress, lowering anxiety, and improving immunity under stressful conditions.

본 발명의 일구현예에 따르면 건열 처리만 한 로스팅 쌀눈 및 건열 처리한 쌀눈을 유산균 발효한 유산균 발효 쌀눈의 가바 함량과 비교한 결과, 발효 전의 쌀눈은 100g당 약 75 mg의 가바를 함유하고 있었다면 유산균 발효 후의 쌀눈의 가바함량이 30%가량 증진되었음을 확인하였다.According to one embodiment of the present invention, as a result of comparing the GABA content of roasted rice buds and dry heat treated rice buds with lactic acid bacteria fermented rice buds with only dry heat treatment, if the rice buds before fermentation contained about 75 mg of GABA per 100 g, lactic acid bacteria It was confirmed that the GABA content of rice sprouts after fermentation was increased by about 30%.

본 발명은 다른 양태로 상기 분말식품을 포함하는 식품 조성물에 관한 것이다.In another aspect, the present invention relates to a food composition comprising the powdered food.

상기 식품 조성물은 기능성 식품(functional food), 영양보조제(nutritional supplement), 건강기능(성)식품(health functional food) 및 식품 첨가제(food additives) 등의 모든 형태를 포함한다. 상기 유형의 식품 조성물은 당업계에 공지된 통상적인 방법에 따라 다양한 형태로 제조할 수 있다. The food composition includes all forms such as functional food, nutritional supplement, health functional food and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

상기 기능성 식품으로는 음료(알콜성 음료 포함), 과실 및 그의 가공식품(예: 과일통조림, 병조림, 잼, 마멀레이드 등), 어류, 육류 및 그 가공식품(예: 햄, 소시지콘비이프 등), 빵류 및 면류(예: 우동, 메밀국수, 라면, 스파게티, 마카로니 등), 과즙, 각종 드링크, 쿠키, 엿, 유제품(예: 버터, 치이즈 등), 식용 식물유지, 마아가린, 식물성 단백질, 레토르트 식품, 냉동식품, 각종 조미료(예: 된장, 간장, 소스 등) 등에 본 발명의 분말식품을 첨가하여 제조할 수 있고, 바람직한 일 예로 음료 조성물 형태로 제조될 수 있다.Examples of the functional food include beverages (including alcoholic beverages), fruits and their processed foods (eg, canned fruit, bottled food, jam, marmalade, etc.), fish, meat and their processed foods (eg, ham, sausage corned beef, etc.), Bread and noodles (e.g. udon, buckwheat noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, taffy, dairy products (e.g. butter, cheese, etc.), edible vegetable oil, margarine, vegetable protein, retort food , It can be prepared by adding the powdered food of the present invention to frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.), and can be prepared in the form of a beverage composition as a preferred example.

상기 건강기능(성)식품(health functional food)은 특정보건용 식품(food for special health use, FoSHU)과 동일한 용어로, 영양 공급 외에도 생체조절기능이 효율적으로 나타나도록 가공된 의학, 의료효과가 높은 식품을 의미한다. 여기서 '기능(성)'은 인체의 구조 및 기능에 대하여 영양소를 조절하거나 생리학적 작용 등과 같은 보건용도에 유용한 효과를 얻는 것을 의미한다. 건강식품(health food)은 일반식품에 비해 적극적인 건강 유지나 증진 효과를 가지는 식품을 의미하고, 건강보조식품(health supplement food)은 건강보조 목적의 식품을 의미한다. 경우에 따라, 건강기능식품, 건강식품, 건강보조식품의 용어는 혼용될 수 있다. The health functional food (health functional food) is the same term as food for special health use (FoSHU), and in addition to supplying nutrients, it is processed to efficiently display bioregulatory functions, and has high medical effects. means food. Here, 'function (sex)' means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. Health food refers to food that has a more active health maintenance or promotion effect than general food, and health supplement food refers to food for the purpose of supplementing health. In some cases, the terms health functional food, health food, and health supplement food may be used interchangeably.

본 발명의 식품 조성물은 당업계에서 통상적으로 사용되는 방법에 의하여 제조 가능하며, 당업계에서 통상적으로 첨가하는 원료 및 성분을 첨가하여 제조할 수 있다. 또한, 상기 식품 조성물은 식품으로 인정되는 제형이면 다양한 형태의 제형으로 제한 없이 제조될 수 있다. 또한, 상기 식품 조성물은 생리학적으로 허용 가능한 담체를 추가로 포함할 수 있는데, 담체의 종류는 특별히 제한되지 않으며 당해 기술 분야에서 통상적으로 사용되는 담체라면 어느 것이든 사용할 수 있다. 또한, 상기 식품 조성물은 식품 조성물에 통상 사용되어 냄새, 맛, 시각 등을 향상시킬 수 있는 추가 성분을 포함할 수 있다.The food composition of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art. In addition, the food composition can be prepared without limitation in various types of formulations as long as they are recognized as food. In addition, the food composition may further include a physiologically acceptable carrier, the type of carrier is not particularly limited, any carrier commonly used in the art may be used. In addition, the food composition may include additional ingredients that are commonly used in food compositions and can improve smell, taste, and vision.

본 발명은 또 다른 양태로 1) 쌀눈에 혼합유산균을 접종하고 발효시켜 유산균 발효 쌀눈을 제조하는 단계; 및 2) 상기 유산균 발효 쌀눈에 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 배합하는 단계; 를 포함하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법에 관한 것이다.In another aspect of the present invention, 1) inoculating mixed lactic acid bacteria into rice buds and fermenting them to prepare lactic acid bacteria fermented rice buds; and 2) mixing pea protein, oatmeal powder, soymilk powder, green tea powder, and sugar with the lactic acid bacteria fermented rice buds; It relates to a method for producing powdered food for beverage production with improved GABA content, including.

상기 단계 1)의 쌀눈은 140 ~ 180℃에서 7 ~ 12분간 건열 처리된 것일 수 있다. 쌀눈은 도정된 직후부터 리폭시게나제(LPO)라는 효소에 의해 산패가 발생되는데 이를 지연시키기 위해 냉동건조 또는 열-처리 안정화 과정을 거쳐야 쌀눈의 유익한 성분을 유지할 수 있다. 이에 140 ~ 180℃에서 7 ~ 12분 동안 건열 처리하여 안정화하는 것이 좋으며, 더욱 바람직하게는 160℃에서 9분 동안 건열 처리하는 것이 좋으나 이에 제한되는 것은 아니다. 이는 140℃ 미만에서 5분 미만으로 건열 처리할 경우에는 리폭시게나제의 활성능이 여전히 살아 있게 되어 안정화하기 어려우며, 180℃ 초과에서 15분초과 건열 처리한 경우에는 배아가 타게 되어 배아 함유 지방질은 물론 감마 오리자놀, 토코페롤, 토코트리엔올, 피토스테롤, 가바 함량 등 유효성분들을 해칠수 있다.The rice buds in step 1) may be dry heat treated at 140 to 180 ° C. for 7 to 12 minutes. Rice buds are rancid by an enzyme called lipoxygenase (LPO) immediately after milling. Therefore, it is good to stabilize by dry heat treatment at 140 to 180 ° C. for 7 to 12 minutes, more preferably dry heat treatment at 160 ° C. for 9 minutes, but is not limited thereto. When dry heat treatment is performed at less than 140°C for less than 5 minutes, the active ability of lipoxygenase is still alive and it is difficult to stabilize, and when dry heat treatment is performed at more than 180°C for more than 15 minutes, the embryo is burned and the fat contained in the embryo as well as It can harm active ingredients such as gamma oryzanol, tocopherol, tocotrienol, phytosterol, and GABA content.

상기 단계 1)의 혼합유산균은 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 플란타룸(Lactobacillus plantarum), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 애시도필러스(Lactobacillus acidophilus), 락토바실러스 퍼멘텀(Lactobacillus fermentum), 비피도박테리움 롱검(Bifidobacterium longum) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)로 이루어진 군에서 선택되는 1종 이상을 혼합 배양한 것일 수 있다. 상기 유산균은 쌀눈이 가지는 영양학적 성분 및 물성을 개선시킬 수 있도록 발효하기에 적합한 다양한 미생물 조합에 있어 상기 락토바실러스 5종 중에서 선택한 쌀눈 발효 유산균주일 수 있다. 상기 각 유산균의 배합비는 특별히 한정되는 것은 아니다.The mixed lactic acid bacteria in step 1) are Lactobacillus casei , Lactobacillus plantarum , Lactobacillus rhamnosus , Lactobacillus acidophilus, Lactobacillus acidophilus , Lactobacillus fur Mentum ( Lactobacillus fermentum ), Bifidobacterium longum ( Bifidobacterium longum ) and Streptococcus thermophilus ( Streptococcus thermophilus ) It may be a mixed culture of one or more selected from the group consisting of. The lactic acid bacteria may be a fermented rice germ strain selected from among the 5 types of Lactobacillus in various combinations of microorganisms suitable for fermentation to improve nutritional components and physical properties of rice germ. The blending ratio of each of the above lactic acid bacteria is not particularly limited.

상기 혼합유산균 및 로스팅된 쌀눈을 1 : 1 ~ 2 의 중량 비율로 혼합 발효할 수 있고 바람직하게는 1 : 1.5의 중량 비율일 수 있으나 이에 제한되는 것은 아니다.The mixed lactic acid bacteria and the roasted rice germ may be mixed and fermented at a weight ratio of 1: 1 to 2, preferably at a weight ratio of 1: 1.5, but is not limited thereto.

상기 유산균 발효에 의해 가바 함량이 증진된 유산균 발효 쌀눈은 동결건조하여 분말화한 후, 분말 그 자체로 상품화하거나 타블렛 또는 과립형태로 제조되어 상품화할 수 있다. The lactic acid bacteria-fermented rice bran whose GABA content is increased by the lactic acid bacteria fermentation can be freeze-dried and powdered, and then commercialized as powder itself or manufactured in the form of tablets or granules.

상기 단계 2)의 배합은 유산균 발효 쌀눈에 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 45 내지 65 rpm에서 5 내지 15분간 수행할 수 있고, 바람직하게는 54 ~ 55rpm에서 10분간 배합할 수 있으나 이에 제한되는 것은 아니다. 상기 단계 2)에서 고구마 분말 및 단호박 분말을 더 포함하거나, 검정깨 분말 및 볶은흑미 분말을 더 포함하여 배합할 수 있다.The blending of step 2) may be performed by mixing pea protein, oatmeal powder, soymilk powder, green tea powder, and sugar with lactic acid bacteria fermented rice bran at 45 to 65 rpm for 5 to 15 minutes, preferably at 54 to 55 rpm for 10 minutes. It can be, but is not limited thereto. In step 2), sweet potato powder and sweet pumpkin powder may be further included, or black sesame powder and roasted black rice powder may be further included.

이하 실시예를 통하여 본 발명을 더욱 상세하게 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것으로 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.The present invention will be described in more detail through the following examples. These examples are only intended to illustrate the present invention, and the scope of the present invention is not to be construed as being limited by these examples.

실시예 1: 유산균 발효 쌀눈의 제조Example 1: Preparation of lactic acid bacteria fermented rice bran

경기도 화성에서 재배되어 2021년 7월에 생산된 쌀을 도정하는 과정에서 얻은 쌀눈을 정제수로 1~2회 세척하고 이물질 및 오염물질을 제거하였다. 그 다음, 쌀눈의 산패를 방지하기 위해 회전식 로스팅기를 이용하여 160℃에서 9분 동안 건열 처리하였다. The rice buds obtained in the process of milling rice grown in Hwaseong, Gyeonggi-do and produced in July 2021 were washed once or twice with purified water and foreign substances and contaminants were removed. Then, dry heat treatment was performed at 160 ° C. for 9 minutes using a rotary roaster to prevent rancidity of the rice buds.

메디오젠에서 구입한 혼합유산균[락토바실러스 카제이(Lactobacillus casei), 락토바실러스 플란타룸(Lactobacillus plantarum), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 애시도필러스(Lactobacillus acidophilus), 락토바실러스 퍼멘텀(Lactobacillus fermentum), 비피도박테리움 롱검(Bifidobacterium longum) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)]을 MRS broth 배지에서 18시간 전배양한 다음 전배양액을 동일한 MRS broth 배지에 접종하고 10시간 중간배양을 실시하였다. 중간배양액을 각 1Х109 CFU/ml 농도로 접종하고 37℃ 배양기에서 24시간 동안 배양한 유산균 혼합액을 준비하였다.Mixed lactic acid bacteria purchased from Mediogen [ Lactobacillus casei , Lactobacillus plantarum, Lactobacillus rhamnosus , Lactobacillus acidophilus , Lactobacillus acidophilus] Mentum ( Lactobacillus fermentum ), Bifidobacterium longum ( Bifidobacterium longum ) and Streptococcus thermophilus ( Streptococcus thermophilus )] were pre-cultured in MRS broth medium for 18 hours, then the pre-culture was inoculated into the same MRS broth medium and 10 hours intermediate Cultivation was carried out. The intermediate culture medium was inoculated at each concentration of 1Х10 9 CFU/ml, and a mixture of lactic acid bacteria cultured in a 37°C incubator for 24 hours was prepared.

혼합물 총 중량 기준으로, 상기 로스팅한 쌀눈 60 중량% 및 유산균 혼합액40 중량%를 혼합한 혼합물을 30℃의 온도에서 24시간 동안 발효하고, 80℃의 온도에서 24시간 동안 살균한 다음 -35℃에서 동결건조 및 분쇄하여 유산균 발효 쌀눈을 제조하였다(도 1). Based on the total weight of the mixture, a mixture of 60% by weight of the roasted rice germ and 40% by weight of the lactic acid bacteria mixture was fermented at a temperature of 30 ° C for 24 hours, sterilized at a temperature of 80 ° C for 24 hours, and then at -35 ° C Lactic acid bacteria fermented rice buds were prepared by freeze-drying and grinding (FIG. 1).

실시예2: 유산균 발효 쌀눈의 가바 함량 증진 효과Example 2: GABA content enhancement effect of lactic acid bacteria fermented rice bran

실시예 1의 방법으로 제조된 유산균 발효를 한 쌀눈과 유산균 발효 전 로스팅된 쌀눈 두 샘플에 대해 LC-MS/MS(SCIEX TRIPLE QUAD 4500)를 이용하여 하기 표 1의 HPLC 측정 조건으로 가바(GABA)함량을 측정하였다.GABA under the HPLC measurement conditions in Table 1 below using LC-MS / MS (SCIEX TRIPLE QUAD 4500) for two samples of lactic acid bacteria fermentation prepared by the method of Example 1 and roasted rice buds before lactic acid bacteria fermentation content was measured.

검출기detector MS/MS (ABSCIEX Q4500)MS/MS (ABSCIEX Q4500) 컬럼column 인트라다 아미노산(5 μm, 100 Х 3 mm)Intrada amino acids (5 μm, 100 Х 3 mm) 이동상mobile phase 0.1 % 아세토니트릴의 포름산Formic acid in 0.1% acetonitrile 100 mM 포름산암모늄/아세토니트릴 (8/2, v/v)100 mM ammonium formate/acetonitrile (8/2, v/v) 유속flow rate 0.5 mL/min0.5 mL/min 주입량injection volume 5 μL5 μL

측정한 값을pure GABA(γ-Aminobutyric acid)를 표준으로 하여 계산하고 표 2에 유산균 발효 쌀눈의 가바 함량 변화를 나타냈다.The measured value was calculated using pure GABA (γ-Aminobutyric acid) as a standard, and Table 2 shows the change in GABA content of lactic acid bacteria fermented rice germ.

유산균 발효 전 쌀눈
Rice bran before fermentation with lactic acid bacteria
유산균 발효 쌀눈Lactobacillus fermented rice bran
가바 함량(mg/100g)GABA content (mg/100g) 75.08 mg/100g75.08mg/100g 99.25 mg/100g99.25mg/100g

표 2에서 보는 바와 같이, 유산균 발효 전 쌀눈에 비하여 유산균 발효 쌀눈의 가바 함량이 높게 나타나 유산균 발효를 통해 쌀눈의 가바 함량이 증진되었음을 확인하였다.As shown in Table 2, the GABA content of the lactic acid bacteria fermented rice buds was higher than that of the rice buds before lactic acid bacteria fermentation, confirming that the GABA content of the rice buds was enhanced through lactic acid bacteria fermentation.

실시예3: 유산균 발효 쌀눈이 함유된 분말 제조Example 3: Preparation of powder containing lactic acid bacteria fermented rice bran

실시예1에서 제조한 유산균 발효 쌀눈의 동결건조물을 첨가하여 도 2 및 도 3의 모식도에 따라 건강 기능성 제품을 위한 분말을 제조하였다.A powder for a health functional product was prepared according to the schematic diagrams of FIGS. 2 and 3 by adding the freeze-dried product of the lactic acid bacteria fermented rice buds prepared in Example 1.

각 제품별 재료 중량 배합비를 표 3에 나타냈다.Table 3 shows the material weight blending ratio for each product.

재료(생산 또는 구입처)Material (produced or purchased) 첨가량
(단위:%)
Amount added
(unit:%)
재료(생산 또는 구입처)Material (produced or purchased) 첨가량
(단위:%)
Amount added
(unit:%)
오트고구마 분말Oat Sweet Potato Powder 오트흑임자 분말Oat Black Sesame Powder 완두콩 단백질(Roquette) 분말Pea Protein (Roquette) Powder 2525 완두콩 단백질(Roquette) 분말Pea Protein (Roquette) Powder 2424 오트밀 분말(OEM, 유성식품)Oatmeal Powder (OEM, Oily Food) 1717 오트밀 분말(OEM, 유성식품)Oatmeal Powder (OEM, Oily Food) 2626 유기농 설탕(PTCoop)Organic Sugar (PTCoop) 2323 유기농 설탕(PTCoop)Organic Sugar (PTCoop) 2626 고구마 분말(세계에프엘)Sweet Potato Powder (World FL) 24.224.2 검정깨 분말(인그린)Black Sesame Powder (Ingreen) 77 두유 분말(찬슬)Soy Milk Powder (Chanseul) 55 두유 분말(찬슬)Soy Milk Powder (Chanseul) 99 단호박 분말(푸른터)Sweet Pumpkin Powder (Pureungteo) 55 볶은흑미 분말(유성식품)Roasted Black Rice Powder (Oily Food) 77 유산균 발효 쌀눈(OEM, 미존)Lactic acid bacteria fermented rice sprouts (OEM, MIZON) 0.50.5 유산균 발효 쌀눈(OEM, 미존)Lactic acid bacteria fermented rice sprouts (OEM, MIZON) 0.50.5 녹차 분말(보성제다)Green Tea Powder (Boseongjeda) 0.30.3 녹차 분말(보성제다)Green Tea Powder (Boseongjeda) 0.50.5 합계Sum 100100 합계Sum 100100

표 3의 배합비로 각 재료를 배합기에서 54 ~ 55 RPM으로 10분동안 배합하여 오트고구마 분말 및 오트흑임자 분말을 제조하였다(도 4).Oat sweet potato powder and oat black sesame powder were prepared by blending each material in a blender at 54 to 55 RPM for 10 minutes in the blending ratio of Table 3 (FIG. 4).

실험예1: 오트고구마 분말 및 오트흑임자 분말의 수용해성Experimental Example 1: Water solubility of oat sweet potato powder and oat black sesame powder

250 ml비커에 30℃의 미지근한 물 100ml을 각각 채우고 상기 실시예3의 오트고구마맛 분말 25g 및 오트흑임자맛 분말 25g을 각각 투입한 다음, 650rpm에서 완전히 용해될 때까지의 시간을 3회에 걸쳐 측정한 평균값을 표 4에 나타내었다.100 ml of lukewarm water at 30 ° C was filled in a 250 ml beaker, respectively, and 25 g of oat sweet potato flavor powder and 25 g of oat black sesame flavor powder of Example 3 were added, respectively, and then the time until completely dissolved at 650 rpm was measured three times. One average value is shown in Table 4.

샘플명sample name 측정 시간measurement time 평균average 1차Primary 2차Secondary 3차tertiary 오트고구마oat sweet potato 2분 1초2 minutes 1 second 1분 22초1 minute 22 seconds 1분 52초1 minute 52 seconds 1분 35초1 minute 35 seconds 오트흑임자oat black sesame 2분 53초2 minutes 53 seconds 2분 37초2 minutes 37 seconds 2분 48초2 minutes 48 seconds 2분 46초2 minutes 46 seconds

표 4를 참고하면, 오트고구마 분말과 오트흑임자 분말 모두 수용해성이 우수한 것을 확인하였다. Referring to Table 4, it was confirmed that both oat sweet potato powder and oat black sesame powder had excellent water solubility.

실험예2: 관능품질평가Experimental Example 2: Sensory quality evaluation

실시예3에서 제조한 분말 25g을 물 125ml에 용해시켜 고구마맛 음료 및 흑임자맛 음료를 제조하고, 표 5의 10~60대 남녀 21명(남 11명, 여 10명)을 대상으로 관능평가를 실시하였다(2021년 10월 28일).25 g of the powder prepared in Example 3 was dissolved in 125 ml of water to prepare a sweet potato-flavored beverage and a black sesame-flavored beverage, and sensory evaluation was performed on 21 men and women in their 10s to 60s (11 males and 10 females) in Table 5. It was conducted (October 28, 2021).

  female 인원personnel other 인원personnel 연령대age group 1010 0 0 1010 33 2020 22 2020 1One 3030 1One 3030 1One 4040 22 4040 1One 5050 33 5050 22 6060 22 6060 33 bout 1010 bout 1111

고구마맛 음료는 시료 A, 흑임자맛 음료는 시료 B, 비교군으로 S사의 단백질보충제 1종을 시료 C로 표기하여 관능품질평가를 진행하고, 구체적인 결과를 도 5에 나타냈다.Sweet potato-flavored beverage was sample A, black sesame-flavored beverage was sample B, and one protein supplement from S company was marked as sample C as a comparative group, and sensory quality evaluation was performed, and specific results are shown in FIG. 5.

도 5를 참고하면, 음료의 점도 항목(도 5a 및 도 5b)에서 진한 정도는 고구마맛 음료(시료 A)의 경우 “조금 강함”(38.10%), 흑임자맛 음료(시료 B)의 경우 "매우 진함"(57.14%), 비교군(시료 C)의 경우 "보통"(38.10%)이라는 평가가 가장 많았다.Referring to FIG. 5, the degree of concentration in the viscosity items of the beverage (FIGS. 5a and 5b) was "slightly strong" (38.10%) for the sweet potato-flavored beverage (sample A) and "very strong" for the black sesame-flavored beverage (sample B). Darkness" (57.14%), and in the case of the control group (sample C), "average" (38.10%) was the most common.

음료의 향 항목(도 5c 및 도 5d)에서 향의 강한 정도는 고구마맛 음료(시료 A)는 "매우 강함" (38.10%)으로, 흑임자맛 음료(시료 B)는 "보통"(42.86%), 비교군(시료 C)은 28.57%의 빈도로 "매우 강함"및 "보통"으로 평가되었다.In terms of flavor of beverages (FIGS. 5c and 5d), the sweet potato-flavored beverage (sample A) was "very strong" (38.10%) and the black sesame-flavored beverage (sample B) was "normal" (42.86%). , the control group (sample C) was evaluated as "very strong" and "normal" with a frequency of 28.57%.

시료의 목넘김 항목(도 5e 및 도 5f))에서 고구마맛 음료(시료 A)는 목넘김이 "조금 진함"(47.62%), 흑임자맛 음료(시료 B)는 "매우 진함"(38.10%), 비교군(시료 C)은 "보통"(57.14%)으로 가장 많이 평가되었다.In the throat item of the samples (FIGS. 5e and 5f)), the sweet potato-flavored beverage (sample A) had a "slightly thick" (47.62%) and black sesame-flavored beverage (sample B) had a "very thick" (38.10%), comparison. The group (sample C) was rated the most as “fair” (57.14%).

음료의 맛 항목(도 5g 및 도 5h)은 고소한 맛과 단 맛으로 나누어 평가를 진행하였다. 고소한 맛에서 고구마맛 음료(시료 A)의 경우 "보통"(61.90%), 흑임자맛 음료(시료 B)의 경우 "매우 진함"(42.86%), 비교군(시료 C)은 "조금 진함"(42.86%)으로 가장 많이 평가되었고, 단 맛에서 고구마맛 음료(시료 A)는 보통(47.62%), 흑임자맛 음료(시료 B)는 "보통"(38.10%) 및 "조금 약함"(38.10%), 비교군(시료 C)은 "매우 진함"(38.10%)으로 가장 많이 평가되었다.The taste items of the beverage (FIGS. 5g and 5h) were evaluated by dividing them into savory taste and sweet taste. In the savory taste, "Normal" (61.90%) for the sweet potato-flavored beverage (Sample A), "Very thick" (42.86%) for the black sesame-flavored beverage (Sample B), and "slightly thick" (Sample C) 42.86%), sweet potato-flavored beverage (Sample A) was rated the most in terms of sweetness (47.62%), and black sesame-flavored beverage (Sample B) was rated as "average" (38.10%) and "slightly weak" (38.10%). , The control group (sample C) was rated the most as "very dark" (38.10%).

시료의 전체적인 기호도(도 5i)는 고구마맛 음료(시료 A)의 경우"보통"(28.57%)이라는 평가가 가장 많았고, 흑임자맛 음료(시료 B)의 경우 "매우 좋음"(33.33%), 비교군(시료 C)의 경우는 "조금 좋음"(28.57%)의 평가가 가장 많았다. As for the overall acceptability of the samples (FIG. 5i), the sweet potato-flavored beverage (sample A) was evaluated as "normal" (28.57%), and the black sesame-flavored beverage (sample B) was evaluated as "very good" (33.33%), comparison. In the case of the group (sample C), the evaluation of "slightly good" (28.57%) was the highest.

각 관능품질평가 항목의 평균값 및 표준편차를 표 6에 나타내고 실험군을 비교 분석하였다.Table 6 shows the average value and standard deviation of each sensory quality evaluation item, and the experimental groups were compared and analyzed.

공정process 고구마맛(시료 A)Sweet potato flavor (Sample A) 흑임자맛(시료 B)Black Sesame Flavor (Sample B) 비교군(시료 C)Comparative group (Sample C) 평균average 표준 편차Standard Deviation 평균average 표준 편차Standard Deviation 평균average 표준 편차Standard Deviation 점도viscosity 시료의 점도viscosity of the sample 3.903.90 0.890.89 4.384.38 0.800.80 2.982.98 1.251.25 점도의 선호도Viscosity Preference 3.603.60 1.181.18 3.643.64 1.261.26 3.383.38 1.281.28 incense 시료의 향aroma of sample 3.773.77 1.201.20 3.313.31 1.051.05 3.513.51 1.311.31 향의 선호도scent preference 3.573.57 1.241.24 3.053.05 1.231.23 3.603.60 1.171.17 taste 고소한 맛savory taste 3.643.64 0.850.85 4.134.13 0.930.93 3.323.32 1.321.32 단맛sweetness 3.483.48 1.031.03 2.802.80 0.930.93 3.863.86 1.281.28 맛의 기호도taste preference 3.603.60 1.031.03 3.553.55 1.091.09 3.033.03 0.870.87 목넘김Throat 시료의 목넘김swallowing of the sample 4.104.10 0.830.83 3.903.90 1.221.22 3.243.24 1.001.00 목넘김의 기호도The sign of passing the throat 3.383.38 1.071.07 3.523.52 1.121.12 3.433.43 0.980.98 전체적인 기호도overall sign 3.443.44 1.201.20 3.573.57 1.401.40 3.173.17 1.241.24

평균치를 비교하였을 때, 음료의 점도가 큰 흑임자맛 음료의 선호도가 가장 좋았고, 점도가 적은 비교군의 선호도는 낮았다When comparing the average values, the preference for the black sesame-flavored beverage with high viscosity was the highest, and the preference for the comparative group with low viscosity was low.

맛의 기호도는 고구마맛 음료 및 흑임자맛 음료가 각각 3.60 및 3.55로 유사한 수준의 높은 선호도를 나타냈으며, 단 맛이 가장 강하다는 평가를 받은 비교군의 경우 맛의 선호도가 가장 낮게 나타나 과한 단 맛이 선호도를 떨어뜨린 다는 것을 확인하였다. As for taste preference, the sweet potato-flavored beverage and the black sesame-flavored beverage were 3.60 and 3.55, respectively, showing a similar high level of preference. It was confirmed that the preference decreased.

음료의 목넘김의 경우 목넘김의 진한 정도에서 중간 평가를 받은 흑임자맛 음료의 목넘김이 높은 선호도를 나타냈다. In the case of drink swallowing, the black sesame-flavored drink, which received an intermediate evaluation in the thickness of the drink, showed high preference.

음료의 전체적인 기호도는 비교군 < 고구마맛 음료 < 흑임자맛 음료 순으로 나타났는데, 점도가 높으며 고소한 맛이 강하고 단맛이 약한 흑임자맛의 기호도가 가장 우세했고, 점도가 낮으면서 단맛이 강한 비교군의 전체적인 기호도는 가장 낮게 나타났다.The overall acceptability of the beverages was in the order of comparison group < sweet potato-flavored beverage < black sesame-flavored beverage. Acceptability was the lowest.

종합적으로, 고구마맛 음료, 흑임자맛 음료 및 S사의 단백질보충제를 이용하여 관능평가를 실시한 결과 소비자의 전반적인 기호도를 만족시키는 것은 본 발명의 오트고구마 분말을 이용한 고구마맛 음료(시료 A) 및 오트흑임자 분말을 이용한 흑임자맛 음료(시료 B)인 것으로 분석되었다.Overall, as a result of sensory evaluation using sweet potato-flavored beverages, black sesame-flavored beverages, and protein supplements from Company S, the sweet potato-flavored beverage (sample A) and oat black sesame powder using the oat sweet potato powder of the present invention satisfy the overall preference of consumers. It was analyzed to be a black sesame flavored beverage (sample B) using.

Claims (16)

유산균 발효 쌀눈, 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 포함하는, 가바 함량이 향상된 음료제조용 분말식품.
A powdered food for preparing a beverage with improved GABA content, comprising lactic acid bacteria fermented rice bran, pea protein, oatmeal powder, soymilk powder, green tea powder and sugar.
제1항에 있어서,
상기 유산균 발효 쌀눈은 0.3 ~ 1 중량부, 완두콩 단백질은 20 ~ 35 중량부, 오트밀 분말은 15 ~ 30 중량부, 두유 분말은 3 ~ 15 중량부, 녹차 분말은 0.1 ~ 1 중량부, 설탕은 20 ~ 35 중량부로 포함되는 것인, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 1,
0.3 to 1 part by weight of the lactic acid bacteria fermented rice buds, 20 to 35 parts by weight of pea protein, 15 to 30 parts by weight of oatmeal powder, 3 to 15 parts by weight of soy milk powder, 0.1 to 1 part by weight of green tea powder, and 20 parts by weight of sugar A powdered food for preparing a beverage with improved GABA content, which is contained in ~ 35 parts by weight.
제1항에 있어서,
상기 조성물은 고구마 분말 및 단호박 분말을 더 포함하는, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 1,
The composition further comprises sweet potato powder and sweet pumpkin powder, powdered food for preparing a beverage with improved GABA content.
제3항에 있어서,
상기 고구마 분말은 20 ~ 30 중량부, 단호박 분말은 3 ~ 10 중량부로 포함되는 것인, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 3,
The sweet potato powder is 20 to 30 parts by weight, and the sweet pumpkin powder is contained in 3 to 10 parts by weight, a powder food for manufacturing a beverage with improved GABA content.
제1항에 있어서,
상기 조성물은 검정깨 분말 및 볶은흑미 분말을 더 포함하는, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 1,
The composition further comprises black sesame powder and roasted black rice powder, powdered food for preparing a beverage with improved GABA content.
제5항에 있어서,
상기 검정깨 분말은 3 ~ 15 중량부, 볶은흑미 분말은 3 ~ 15 중량부로 포함되는 것인, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 5,
The black sesame powder is 3 to 15 parts by weight and the roasted black rice powder is contained in 3 to 15 parts by weight, a powder food for preparing a beverage with improved GABA content.
제1항에 있어서,
상기 유산균 발효 쌀눈은 가바를 100g 당 90 내지 110mg 포함하는 것인, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 1,
The lactic acid bacteria fermented rice bran contains 90 to 110 mg of GABA per 100 g, a powder food for preparing a beverage with improved GABA content.
제3항에 있어서,
상기 분말식품 100중량%에 대하여, 유산균 발효 쌀눈 0.5 중량%, 완두콩 단백질 25 중량%, 오트밀 분말 17 중량%, 두유 분말 5 중량%, 녹차 분말 0.3 중량%, 설탕 23 중량%, 고구마 분말 24.2 중량% 및 단호박 분말 5 중량%을 포함하는 것인, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 3,
Based on 100% by weight of the powdered food, 0.5% by weight of lactic acid bacteria fermented rice bran, 25% by weight of pea protein, 17% by weight of oatmeal powder, 5% by weight of soymilk powder, 0.3% by weight of green tea powder, 23% by weight of sugar, 24.2% by weight of sweet potato powder And a powdered food for preparing a beverage with improved GABA content, comprising 5% by weight of sweet pumpkin powder.
제5항에 있어서,
상기 분말식품 100중량%에 대하여, 유산균 발효 쌀눈 0.5 중량%, 완두콩 단백질 25 중량%, 오트밀 분말 17 중량%, 두유 분말 5 중량%, 녹차 분말 0.3 중량%, 설탕 23 중량%, 검정깨 분말 7중량% 및 볶은흑미 분말 7중량%을 포함하는 것인, 가바 함량이 향상된 음료제조용 분말식품.
According to claim 5,
Based on 100% by weight of the powdered food, 0.5% by weight of lactic acid bacteria fermented rice bran, 25% by weight of pea protein, 17% by weight of oatmeal powder, 5% by weight of soymilk powder, 0.3% by weight of green tea powder, 23% by weight of sugar, 7% by weight of black sesame powder % and 7% by weight of roasted black rice powder.
제1항 내지 제9항 중 어느 한 항의 분말식품을 포함하는 식품 조성물.
A food composition comprising the powdered food according to any one of claims 1 to 9.
1) 쌀눈에 혼합유산균을 접종하고 발효시켜 유산균 발효 쌀눈을 제조하는 단계; 및
2) 상기 유산균 발효 쌀눈에 완두콩 단백질, 오트밀 분말, 두유 분말, 녹차 분말 및 설탕을 배합하는 단계; 를 포함하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법.
1) inoculating mixed lactic acid bacteria into rice buds and fermenting them to prepare lactic acid bacteria fermented rice buds; and
2) mixing pea protein, oatmeal powder, soymilk powder, green tea powder, and sugar with the lactic acid bacteria fermented rice bran; A method for producing powdered food for beverage production with improved GABA content, comprising:
제11항에 있어서,
상기 단계 1)의 쌀눈은 140 ~ 180℃에서 7 ~ 12분간 건열 처리된 것을 특징으로 하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법.
According to claim 11,
The rice sprouts in step 1) are dry heat treated at 140 to 180 ° C. for 7 to 12 minutes.
제11항에 있어서,
상기 단계 1)의 혼합유산균은 락토바실러스 카제이(Lactobacillus casei), 락토바실러스 플란타룸(Lactobacillus plantarum), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 애시도필러스(Lactobacillus acidophilus), 락토바실러스 퍼멘텀(Lactobacillus fermentum), 비피도박테리움 롱검(Bifidobacterium longum) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)로 이루어진 군에서 선택되는 1종 이상을 혼합 배양한 것을 특징으로 하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법.
According to claim 11,
The mixed lactic acid bacteria in step 1) are Lactobacillus casei , Lactobacillus plantarum , Lactobacillus rhamnosus , Lactobacillus acidophilus, Lactobacillus acidophilus , Lactobacillus fur Mentum ( Lactobacillus fermentum ), Bifidobacterium longum ( Bifidobacterium longum ) and Streptococcus thermophilus ( Streptococcus thermophilus ) Characterized by mixing and culturing at least one species selected from the group consisting of, beverage manufacturing powder with improved GABA content Food manufacturing method.
제11항에 있어서,
상기 단계 1)의 발효는 혼합유산균 및 쌀눈을 1 : 1 ~ 2 의 중량 비율로 혼합하여 발효한 것을 특징으로 하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법.
According to claim 11,
The fermentation in step 1) is a method for producing powdered food for beverage production with improved GABA content, characterized in that the mixed lactic acid bacteria and the rice bran are mixed and fermented in a weight ratio of 1: 1 to 2.
제11항에 있어서,
상기 단계 2)의 배합은 45 내지 65 rpm에서 5 내지 15분간 수행되는 것인, 가바 함량이 향상된 음료제조용 분말식품의 제조방법.
According to claim 11,
The blending of step 2) is performed at 45 to 65 rpm for 5 to 15 minutes, a method for producing powdered food for beverage production with improved GABA content.
제11항에 있어서,
상기 단계 2)에서 고구마 분말 및 단호박 분말; 또는 검정깨 분말 및 볶은흑미 분말; 을 더 포함하여 배합하는, 가바 함량이 향상된 음료제조용 분말식품의 제조방법.
According to claim 11,
Sweet potato powder and sweet pumpkin powder in step 2); or black sesame powder and roasted black rice powder; A method for producing a powdered food for preparing a beverage having an improved GABA content, further comprising:
KR1020220141282A 2021-11-30 2022-10-28 Food composition comprising fermented rice germ lactic acid bacteria KR20230081961A (en)

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