KR20230024727A - Process for Preparing breeding chicken using Korean native pure line chicken - Google Patents

Abstract

The present invention relates to a production method of a breeding chicken using Korean native pure line chickens including Hanhyeop Jinsan F line varieties, Hanhyeop Jinsan H line varieties, and Hanhyeop Jinsan line Y line varieties, and the breeding chicken produced by the method. By using the production method of a breeding chicken in the present invention, the breeding chicken with higher economic feasibility and quality than conventional breeding chickens can be produced. Therefore, the present invention can be widely used in the development of high-quality chicken processed foods.

Description

Production method of breeder chicken using Korean native pure line chicken {Process for Preparing breeding chicken using Korean native pure line chicken}

The present invention relates to a method for producing a breeder, and more specifically, the present invention relates to a method for producing a breeder using a breed of the Hanhyeop Jinsan F strain, a breed of the Hanhyeop Jinsan H strain, and a strain of the Hanhyeop Geumsan Y strain, which are native chickens. and breeders produced by the method.

Native chickens have been passed down in Korea since ancient times, and they almost disappeared around the 1980s due to lower productivity and economic efficiency than broilers, laying hens, and improved breeds imported from abroad during the Korean War and the era of industrialization. In recent years, the demand for native chickens has increased as the number of consumers who are looking for native breeds that suit our appetite rather than foreign improved breeds has increased as the tendency to prefer livestock products with good taste and quality has increased. However, the appearance of fake native chickens made by crossbreeding between laying hens and imported broilers, as well as various similar breed chickens and hybrid chickens of unknown origin, led to consumers' distrust of native chickens. In addition, traditional chickens so far lack uniformity in quality, have a low degree of improvement for production traits, and have been difficult to standardize and industrialize production because the system has not been established.

Accordingly, the National Institute of Animal Science and Technology collected native chickens that had been scattered across the country since 1992 and collected reddish-brown, tanned, black, white, and grayish-brown native chickens in 20 generations, one generation per year, focusing on appearance and body shape. Native chickens of pure lineage were restored throughout. The breed restoration was completed in 2007 by increasing the purity of the pure heirs that have been restored so that they do not mix with other breeds for 15 years.

Offspring from crossbreeding between breeds or crossbreeding tend to be superior in traits of livestock, such as growth rate, survival rate and number of eggs, compared to their parents' breeds or strains, which is called heterosis. In general, when breeding using hybrid vigor, broiler systems are used as paternal lines, and egg-mixed species are used as maternal lines because they have good breeding ability as well as egg-laying ability. Using native chicken purebreds completely indigenous to Korea, research has been conducted to produce domestic practical chickens with excellent meat quality and improved growth through crossbred strength, and industrial use while promoting capacity improvement, maintenance and preservation of purebreds. Industrialization research focusing on mass production is being carried out by establishing a production system of pure breed - original breed - breeder - practical chicken, which is the production method of chickens.

For example, Korean Patent Publication No. 10-2006-0038879 discloses 'development of molecular markers for improving Korean native chicken breed characteristics and economic traits', and Korean Patent Publication No. 10-2006-0091874 discloses 'meat quality and A broiler chicken with improved storability and a method for producing the same is disclosed, and Korean Patent Publication No. 10-2013-0131776 discloses "Our Taste Chicken No. 2 and No. 3 produced using domestic chicken pure chicken".

Under this background, the present inventors have made diligent research efforts to develop native chicken breeds with superior marketability using domestic native chicken purebreds. The present invention was completed by developing a method for producing breeders of higher quality than conventional breeders by crossing varieties of the Hanhyeop Geumsan Y line.

One object of the present invention is to provide a method for producing excellent quality breeders using the Hanhyeop Jinsan F strain, the Hanhyup Jinsan H strain, and the Hanhyup Jinsan Y strain.

Another object of the present invention is to provide a breeder produced by the method.

One embodiment of the present invention for achieving the above object provides a method for producing a breeder using a breeder for breeding produced by crossing a variety of the Hanhyup Jinsan F line, a variety of the Hanhyeop Jinsan H line, and a variety of the Hanhyeop Jinsan Y line. do.

Specifically, the breeder production method provided by the present invention includes (a) a male chicken hybrid of Hanhyeop Jinsan F-lineage breed and Hanhyeop Jinsan H-lineage breed; and producing a mother, each of which is a hybrid female chicken of Hanhyeop Jinsan F line or Hanhyeop Jinsan H line breed and Hanhyeop Jinsan Y line breed; and, (b) crossing the male and female lines produced above to obtain a breeder line.

The term "Hanhyeop Jinsan system" of the present invention is one of the breeds of chickens also called Cornish breed (Cornish, Gallus gallus), which has a fast growth rate and is used exclusively for meat. . The shoulders are broad and both sides of the body are rounded. The thighs are round and muscular. Cornish species weigh 3.0 kg at 150 days old, 3.7 kg at 270 days old, and 55-60 g at 270 days old.

The above Hanhyeop Jinsan strains are purebred to the Korea Native Chicken Association in accordance with the Livestock Act for the Protection and Development of Native Livestock and the standards and procedures for native livestock recognition (Notice No. 2015-175 of the Ministry of Agriculture, Food and Rural Affairs) in the Enforcement Rules of the Act. As the official name of the breed identified by , the F line means a breed with brown hair color, and the H line means a breed with black hair color.

The term "Hanhyeop Geumsan Y system" of the present invention is also referred to as "New Hampshire breed", and is a breed of chicken recognized as a breed in 1935 by improving the Rhode Island Red species in New Hampshire, USA. It is not as long as the red species, has a robust constitution, is docile, but does not incubate eggs well. It grows quickly and develops feathers quickly. The color of the feathers is generally walnut, the long tail feathers are black, and the female has a black speckle under the head. there is.

The above-mentioned Hanhyup Geumsan Y system is registered with the Korea Native Chicken Association in accordance with the Livestock Act for the Protection and Raising of Native Livestock and the standards and procedures for native livestock recognition (Notice No. 2015-175 of the Ministry of Agriculture, Food and Rural Affairs) in the Enforcement Rules of the Act. It is the formal name of a breed that has been identified as a purebred.

Normally, in the commercial use of chickens, pure chickens or original breeding chickens can be used as practical chickens, but economic effects are maximized by producing breeders suitable for the purpose of use and producing practical chickens with an optimal breeding system using them. The mating system for producing breeders and practical hens using pure hens appears different depending on the genetic characteristics and breeding direction of pure hens.

The purebred breeds used in the present invention, Hanhyeop Jinsan H line, Hanhyeop Jinsan F line, and Hanhyeop Jinsan Y line, are native chicken purebreds maintained and preserved by Hanhyeop Wonjong Co., Ltd., a participating company of the present invention. The purebred line is a breed that has fixed and improved the appearance color and ability of each line by accumulating pedigree information through artificial insemination so that the father and mother can be known during the breeding process of each generation. It is a breed that has been recognized as a chicken purebred and has been recognized for its breeding history and composition.

The purebred line disclosed in the present invention is a group that maintains breeding characteristics among breeds or inbreeds, and purebreds possessed by Hanhyupwonjong Co., Ltd. are classified into a group with different genetic properties from other identical breeds.

In the step (a), the paternal line is not particularly limited thereto, but as an example, one hybrid male chicken (FH strain) obtained by crossing a Hanhyeop Jinsan F-lineage male chicken and a Hanhyeop Jinsan H-lineage female chicken, Hanhyup It can be a single hybrid male chicken (HF strain) obtained by mating a Jinsan H-lineage male chicken and a Hanhyeop Jinsan F-lineage female chicken.

In the step (a), the mother system is not particularly limited thereto, but as an example, one hybrid female chicken (FY system) obtained by crossing a male chicken of Hanhyeop Jinsan F system and a female chicken of Hanhyeop Geumsan Y system, Hanhyup It can be a first-generation hybrid female chicken (HY strain) obtained by crossing a Jinsan H-lineage male chicken with a Hanhyeop Geumsan Y-lineage female chicken.

In the step (b), the obtained breeder is not particularly limited thereto, but as an example, a breeder (FHFY strain) obtained by crossing a male chicken of FH strain and a female chicken of FY strain, a male chicken of FH strain Breeders obtained by crossing female chickens of HY and HY lines (FHHY lines), breeders obtained by crossing male chickens of HF lines and female chickens of FY lines (HFFY lines), male chickens of HF lines and female chickens of HY lines It can be a breeder (HFHY line) obtained by crossing.

Another embodiment of the present invention provides a breeder produced by the above method.

The breeders provided in the present invention are FHFY lines, FHHY lines, HFFY lines or HFHY lines, and the specific characteristics of these lines are as follows:

The FHFY strain reaches a weight of 800 g after hatching at 33 to 34 days, the age at which a weight of 2200 g is reached at 81 to 83 days, and the feed conversion rate after breeding for 12 weeks is 1.5 to 2.5%.

FHHY strains reach a weight of 800 g after hatching at 31 to 33 days, reach a weight of 2200 g at 78.5 to 79.5 days, and have a feed conversion rate of 2.0 to 3.0% after breeding for 12 weeks.

In the HFFY line, the age to reach 800 g after hatching is 32.5 to 33.5 days, the age to reach 2200 g is 79 to 80 days, and the feed conversion rate after breeding for 12 weeks is 2.0 to 3.0%.

In the HFHY line, the age to reach a weight of 800 g after hatching is 32.5 to 33.5 days, the age to reach a weight of 2200 g is 79.5 to 80.5 days, and the feed conversion rate after breeding for 12 weeks is 2.0 to 2.5%.

Meanwhile, in order to obtain a patent for an animal, the fertilized egg of the animal must be deposited at a government-approved research institute. Since most of the embryos entrusted to the depository need to be stored for a long period of time, first, the state of the embryos must be checked through a viability test of the embryos, and then frozen and stored. The purpose of freezing fertilized eggs is to preserve the lineage of a specific animal for a long period of time, and to restore it by transplanting it to a surrogate mother after thawing if necessary. The basic principle of fertilized egg freezing is to remove intracellular water (cell count) components using a cryoprotectant and osmotic pressure to suppress intracellular ice crystal formation and regain viability after thawing. However, in the case of birds, the fertilized egg is in the form of an egg, and the bird's egg is made of an egg-shell for protection, making it impossible to freeze. Therefore, since it is impossible to deposit fertilized eggs of the breeder provided in the present invention, the present invention enables reproducibility in the production of breeders using breed-fixed pure hens.

Using the breeder production method provided by the present invention, it is possible to produce breeders with higher economic efficiency and quality than conventional breeders, so it can be widely used in the development of high-quality chicken processed foods.

1 is a graph showing the feed conversion rate by breeding period of native chicken breeds produced by the method of the present invention, W is white rice as a control group, HH is commercial native chicken as a comparison group, (a) is 1 week, (b) is 3 weeks, (c) is 5 weeks, (d) is 8 weeks, and (e) shows the results measured for native chickens raised for 12 weeks.
Figure 2 is a graph showing the body weight by breeding period of native chicken breeds produced by the method of the present invention, W is white semi-meal as a control group, HH is commercial native chicken as a comparison group, (a) is 1 week, (b) is 1 week 3 weeks, (c) shows the results measured for native chickens raised for 5 weeks, (d) for 8 weeks, and (e) for 12 weeks.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited to these examples.

Example 1: Production of breeder for breeding and characterization of the produced breeder for breeding

Example 1-1: Production of Breeders for Breeding

Among the native chicken breeds registered in the Livestock Diversity Information System (DAD-IS, FAO), the Hanhyup Jinsan F-lineage breed, the Hanhyup Jinsan H-lineage breed, and the Hanhyup Jinsan-lineage Y-lineage breed are selected, and they are crossed, produced.

The breeders for mating produced above are two paternal breeds and two maternal breeds, respectively, and each breeder is as follows:

Paternal breeding line FH line: 1 hybrid male by mating between Hanhyeop Jinsan F line male and Hanhyeop Jinsan H line female

Paternal breeding line HF line: 1 hybrid male by mating between Hanhyeop Jinsan H lineage male and Hanhyeop Jinsan F lineage female

Maternal breeder HY line: One hybrid female produced by mating Hanhyeop Jinsan H line male and Hanhyeop Geumsan Y line female

Maternal breeder FY line: One hybrid female produced by crossing males of Hanhyeop Jinsan F line and females of Hanhyeop Geumsan Y line

Example 1-2: Characteristic analysis of breeder for breeding

The characteristics of the breeder for breeding produced in Example 1-1 were analyzed.

Example 1-2-1: Color distribution

The feather color of the breeder for breeding produced in Example 1-1 was investigated (Table 1).

As shown in Table 1, it was confirmed that all breeders for breeding showed feather colors in the form of a mixture of yellowish brown, reddish brown, and two colors.

Example 1-2-2: survival rate

For the breeder for breeding produced in Example 1-1, the breeding period is from 0 to 15 weeks of age according to the development process of the chick, and when spawning starts after 16 weeks of age, the survival rate by development stage is investigated by classifying it into a layer. (Table 2).

As shown in Table 2 above, the average survival rate of the growth period compared to admission was 96.0%, and there was no significant difference between the combinations. The average survival rate of the spawning period was 91.3%, which was lower than that of the breeding period.

Example 1-2-3: body weight

For the breeders for mating produced in Example 1-1, the weight of each individual from the time of occurrence to 20 weeks of age was investigated at 2-week intervals, and then during the spawning period, at 4-week intervals up to 40 weeks (Table 3).

As shown in Table 3, the difference in weight according to gender was shown, and it was confirmed that there was no difference in weight between combinations within the same gender in all sections.

Example 1-2-4: Feed intake

For the breeders for mating produced in Example 1-1, the average feed intake per day per animal was analyzed by dividing them into induction period, breeding period, and spawning period (Table 4).

As shown in Table 4 above, the average infertility feed intake per number between breeding combinations was the highest in FH, and low in HY and FY. It was confirmed that there was no difference between the groups within the same gender in feed consumption during incubation and breeding, and no difference in feed intake during spawning in all combinations.

Example 1-2-5: Feed conversion rate

For the breeders for breeding produced in Example 1-1, the feed requirement ratio was analyzed as the ratio of feed intake to weight gain during the breeding period, and as the ratio of feed consumption to egg production from 21 weeks of age to the end of the test. (Table 5).

As shown in Table 5, it was confirmed that the feed conversion rate during the breeding period differed according to male and female and showed a significant difference between female combinations, and in the case of the spawning period, there was no difference between female combinations.

Example 1-2-6: Scattering ability

For the breeder for breeding produced in Example 1-1, the first age, egg production index, daily egg laying rate and 32w egg weight were measured to evaluate egg laying ability (Table 6). At this time, the age of first birth means the age at which the egg production rate of the test system for each mating combination reached 5%; Hen-housed egg production refers to a value obtained by dividing the total number of eggs at the same time by the number of viable eggs at the time of first birth; Hen-day egg production means the ratio of the total number of eggs to the number of live eggs per year from the time of first birth to the end of the assay; 32w mean egg weight at 32 weeks of age.

As shown in Table 6, the average priming age was 161 days, the average spawning rate was 75.2%, the average spawning index was 143.2, and the average 32w egg weight was 55.2g.

Example 2: Production of native chickens through breeding of breeders for breeding and analysis of characteristics of produced native chicken breeds

Example 2-1: Production of native chickens through breeding of breeders for breeding

Four types of native chickens were produced by crossing the two paternal breeders and the maternal breeders produced in Example 1-1 in each combination. The native chickens produced are as follows:

HFFY: Native chicken breed obtained by crossing the paternal breeder HF line and the maternal breeder FY line

HFHY: Native chicken breed obtained by crossing the paternal breeder HF line and the maternal breeder HY line

FHFY: Native chicken breed obtained by crossing the FH line for the father and the FY line for the mother

FHHY: Native chicken breed obtained by crossing the FH line for the father and the HY line for the mother

Example 2-2: Analysis of characteristics of produced native chicken breeds

The characteristics of the four native chicken breeds produced in Example 2-1 were analyzed.

Example 2-2-1: Feed conversion rate

Using the method of Example 1-2-5, the feed conversion rate for each breeding period was analyzed while breeding the four native chicken breeds produced in Example 2-1 for 1 to 12 weeks (FIG. 1) .

1 is a graph showing the feed conversion rate by breeding period of native chicken breeds produced by the method of the present invention, W is white rice as a control group, HH is commercial native chicken as a comparison group, (a) is 1 week, (b) is 3 weeks, (c) is 5 weeks, (d) is 8 weeks, and (e) shows the results measured for native chickens raised for 12 weeks.

As shown in Figure 1, in the first week, the deviation of the feed conversion rate was very large, but after that, the deviation gradually decreased, and the feed conversion rate of the white rice was the lowest, and then HFFY, HFHY, and FHHY were similar, and FHFY was initially Contrary to the similar results, at 5 weeks, the feed conversion rate was higher than that of white rice, the new breed of Samgye breeding combination, and commercial native chickens. It was shown to be similar to the breeding combinations (HFFY, HFHY, FHFY. Afterwards, during the production of practical systems, the new breed native chicken breeding associations HFFY, HFHY, FHHY and commercial native chickens were similar, and the new breed native chicken breeding association FHFY had higher feed than the rest showed the demand rate.

Example 2-2-2: body weight

The body weight was measured while breeding the four native chicken breeds produced in Example 2-1 for 1 to 12 weeks (FIG. 2).

Figure 2 is a graph showing the body weight by breeding period of native chicken breeds produced by the method of the present invention, W is white semi-meal as a control group, HH is commercial native chicken as a comparison group, (a) is 1 week, (b) is 1 week 3 weeks, (c) shows the results measured for native chickens raised for 5 weeks, (d) for 8 weeks, and (e) for 12 weeks.

As shown in Figure 2, the initial live weight was the highest in the new breed of native breed HFHY, and the lowest in commercial native chicken. However, in the case of native Samgye, at 3 weeks of age, White Semi had the highest weight, and the new breed of Samgye HFFY, HFHY, and FHHY had the next highest weight. showed up

Afterwards, the same trend was observed in the production of domestic chicken practical systems until the 8th week. As a result of the 12th week, there was no significant difference in all of the new breed domestic chicken practical systems (HFFY, HFHY, FHFY, FHHY), and the new breed domestic chicken practical chickens HFFY and FHHY were commercial breeds. showed a higher live weight than that of chickens.

Example 2-2-3: growth rate

The age of reaching 800 g and 2200 g of body weight while breeding the four native chicken breeds produced in Example 2-1 was calculated using a trend line (Tables 7 and 8).

As shown in Table 7, in the case of 800 g of age, white rice showed the shortest age at 28.8 days. All breeding combinations of the four native chicken breeds reached 800g about 2 to 1 day shorter than commercial native chickens, and it was confirmed that growth was faster than existing commercial native chickens.

As shown in Table 8, in the case of the age of 2200g, similar to the age of 800g, it was confirmed that all crossing combinations of the four breeds of native chickens showed faster growth than commercial native chickens.

Specifically, FHHY showed an age of at least 4 days, HFFY and HFHY about 3 days, and FHFY about 1 day faster than commercial native chickens, so compared to FHFY, FHHY, HFFY and HFHY are relatively superior. Could know.

Example 2-2-4: Productive

Productivity was evaluated by synthesizing the feed conversion rate analyzed in Example 2-2-1, the weight analyzed in Example 2-2-2, and the newly measured shin length (Table 9).

As shown in Table 9, when looking at the uniformity of body weight, the new breed of native chickens HFFY, HFHY, FHHY and commercial native chickens had a lower coefficient of variation in feed conversion rate compared to white semi-finished rice, showing consistent results, showing high uniformity, In the production of practical chickens, the coefficient of variation of Hanhyup No. 3 was the lowest, but it was confirmed that the new breed of domestic chicken practical chickens also appeared within 3%.

In addition, looking at the weight data, even in live weight, white rice had a higher coefficient of variation than new breed native chickens and commercial native chickens.

The new breed native chicken breeding combination FHHY showed the lowest coefficient of variation in the production of Samgye, followed by HFHY, HFFY, commercial native chicken, and FHFY. It was confirmed that they appeared in the order of HFHY and FHHY.

Example 2-2-5: Manufacturing cost

Manufacturing costs of the four native chicken breeds produced in Example 2-1 were compared (Table 10).

As shown in Table 10, based on white rice, new breeds of native chickens, HFFY, HFHY, and FHHY, were lower than commercial native chickens, and FHFY was higher, indicating that the three breeds could be raised while reducing production costs than commercial native chickens. HFFY, which showed the lowest manufacturing cost, was found to be 44.8 won more expensive than white semi-finished rice. In the practical system, the production cost of the new breed of land chicken HFFY and FHHY was lower than that of the commercial land chicken, and it was found that HFFY had the lowest manufacturing cost in both the Samgye and domestic chicken practical systems.

Example 2-3: Quality Analysis of Produced Native Chicken Breeds

The quality of the breast and leg meat extracted from the four types of native chickens produced in Example 2-1 was analyzed.

Example 2-3-1: Descriptive analysis

For the breasts of the four native chicken breeds produced in Example 2-1, a descriptive analysis was conducted to express the sensory evaluation of the breasts of the candidate line in terms of relative intensity based on HH (5 points) (Table 11).

As shown in Table 11, the savory and sweet flavor showed superior scores in candidate line B than control HH and candidate line C, and the fishy flavor showed a stronger fishy taste in candidate line C than candidate line B. In terms of softness, candidate line B was softer than control HH and Broiler 19, and chewiness of Broiler 19 was chewier than HH and candidate lines C and D. In terms of overall acceptability, candidate line B showed better scores than HH and Broiler19, and candidate line C showed the lowest acceptability. It is known that the flavor of meat depends on the amount of fat, fatty acid composition, reducing sugar and amino acid content of raw meat. Candidate line B had a strong savory and sweet flavor, a weak fishy flavor and the highest fat content, so the softness was consistent with the soft result in the sensory test, and it is judged to have a positive effect on the sensory characteristics.

In addition, for the leg meat of the four native chicken breeds produced in Example 2-1, the sensory evaluation of the candidate line leg meat is expressed in relative strength based on HH (5 points) Descriptive analysis proceeded (Table 12).

As shown in Table 12, the savory and sweet flavor was stronger in candidate line B than C, and the fishy flavor was the weakest in B, although there was no significant difference between the treatment groups. In terms of softness, candidate line B was softer than control group HH and Broiler 19, and chewiness of Broiler 19 was chewier than H, and there was no significant difference from candidate lines [A, B, C, D]. The overall acceptability was analyzed to be similar to that of breast meat, with candidate line B showing the highest score, and candidate line C showing the lowest score, compared to control HH.

In addition, candidate line B had a strong savory and sweet flavor, a weak fishy flavor, and a soft but chewy texture, which was judged to have a positive effect on the overall preference, and was analyzed to show similar results to breast meat.

Example 2-3-2: Antioxidation

The breast meat of the four native chicken breeds produced in Example 2-1 was extracted, stored for 7 days, and then the TBARS value and DPPH radical scavenging ability were measured, and the antioxidant properties were analyzed (Table 13).

As shown in Table 13, there was no significant difference in the TBARS values between control groups HH and Broiler19 and candidate lines [A, B, C, and D] on day 0 and day 7 of storage, and as the storage period increased, all treatments showed significant It was confirmed that it increased to .

In addition, DPPH radical scavenging ability was higher than that of Broiler19 in all of the candidate lines [A, B, C, and D] on day 0 of storage, and on day 7 of storage, it was confirmed that candidate line A showed significantly higher scavenging ability than control HH and Broiler19. .

In addition, the legs of the four native chicken breeds produced in Example 2-1 were removed, stored for 7 days, and then the TBARS value and DPPH radical scavenging ability were measured, and the antioxidant properties were analyzed (Table 14 ).

As shown in Table 14, there was no significant difference in TBARS values between control HH and candidate lines [A, B, C, D] on day 0 and day 7 of storage, and Broiler19 had a higher TBARS than HH and candidate line D on day 0 of storage. The value was significantly high, and at 7 days of storage, it was confirmed that Broiler19 still showed a significantly higher level than HH and candidate lines [A, B, C, D].

In addition, DPPH radical scavenging ability showed no significant difference between control HH and candidate lines [A, B, C, D] on day 0 of storage, and Broiler19 showed the significantly lowest scavenging ability. On the 7th day of storage, candidate line B showed significantly higher scavenging activity than control HH and Broiler19, and candidate lines [A, B, C, D] showed higher DPPH radical scavenging activity than Broiler19, and as the storage period passed, DPPH of Broiler19 It was found that the radical scavenging ability was significantly decreased.

Example 2-3-3: Fatty acid content

The breast meat of the four native chicken breeds produced in Example 2-1 was extracted, and the content of various fatty acids contained therein was measured (Table 15). At this time, as a comparison group, information on broiler broilers (Broiler17) confirmed in 2017 and broiler broilers (Broiler19) confirmed in 2019 was used.

As shown in Table 15, the main fatty acid composition of breast meat is oleic acid (18:1), palmitic acid (16:0), linoleic acid (18:2), stearic acid (18:0), arachidonic acid (20:4 ) was confirmed to appear in order.

In addition, unlike previous research results, native chickens [HH, candidate lines A, B, C, and D] showed higher oleic acid content and lower arachidonic acid content than Broiler19.

In the case of Doscosahexaenoic acid (C22:6, DHA), it acts as a functional factor in vivo, such as improving learning function, preventing circulatory system disease and arteriosclerosis, and is ingested in the form of food or medicine. There was no significant difference between treatment groups, but H showed the highest content. The polyunsaturated fatty acid (PUFA) content reported to be related to umami, deep taste, and flavor was higher in Broiler19 than HH and candidate lines [A, B, C, D], whereas Broiler17 showed a lower content.

In addition, the legs of the four native chicken breeds produced in Example 2-1 were removed, and the contents of various fatty acids contained therein were measured (Table 16).

As shown in Table 16, the main fatty acid composition of leg meat is the same as that of breast meat, oleic acid (18:1), palmitic acid (16:0), linoleic acid (18:2), stearic acid (18:0), arachidonic acid (20:4), and oleic acid and arachidonic acid also showed conflicting results in Broiler19 and Broiler17, as in breast meat, and there was a significant difference between control HH and candidate lines [A, B, C, D]. There was no There was no significant difference in linolenic acid (18:3), an omega-3 (n3) fatty acid, between control HH and Broiler19 and candidate lines [A, B, C, D], and Broiler17 showed a high content. Doscosahexaenoic acid (C22:6, DHA) showed a high content in Broiler19, although there was no significant difference between the control and candidate lines. Polyunsaturated fatty acid (PUFA) content was higher in Broiler19 than in all treatments, and candidate lines [A, B, C, D] and control HH showed significantly higher content than Broiler17.

Example 2-3-4: Free amino acid content

The breast meat of the four native chicken breeds produced in Example 2-1 was extracted, and the content of various free amino acids contained therein was measured (Table 17). At this time, as a comparison group, information on broiler broilers was used.

As shown in Table 17, the content of glutamic acid was the highest in control HH, followed by candidate line D, and Broiler showed a low content. The content of carnosine, which is related to the savory taste of meat, was higher in control HH and candidate lines [A, B, C, D] than in Broiler, and the total content of free amino acids was the highest in control HH and the highest in candidate line C. showed low content. tasty A.A., indicating good taste. The content was significantly higher in Broiler than candidate lines A and C, and there was no significant difference in the other strains, and tasty A.A. / bitter A.A. The ratio confirmed that control H, Broiler, and candidate line D were significantly higher than C.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (10)

(a) A father who is a hybrid male chicken of Hanhyeop Jinsan F line breed and Hanhyeop Jinsan H line breed; and producing a mother, each of which is a hybrid female chicken of Hanhyeop Jinsan F line or Hanhyeop Jinsan H line breed and Hanhyeop Jinsan Y line breed; and,
(b) a method for producing a breeder, comprising the step of crossing the male and female lines produced above to obtain a breeder.
According to claim 1,
The paternal line in step (a) is a single hybrid male chicken (FH strain) obtained by crossing a male chicken of Hanhyeop Jinsan F line and a female chicken of Hanhyeop Jinsan H line.
According to claim 1,
The paternal line in step (a) is a single hybrid male chicken (HF strain) obtained by crossing a Hanhyeop Jinsan H-lineage male chicken and a Hanhyeop Jinsan F-lineage female chicken.
According to claim 1,
The mother line in step (a) is a single hybrid female chicken (FY line) obtained by crossing a male chicken of the Hanhyeop Jinsan F line and a female chicken of the Hanhyeop Geumsan Y line.
According to claim 1,
The mother of step (a) is a single hybrid female chicken (HY system) obtained by crossing Hanhyeop Jinsan H-lineage male chicken and Hanhyeop Geumsan Y-lineage female chicken.
According to claim 1,
The breeder in step (b) is a breeder (FHFY strain) obtained by crossing male chickens of the FH strain and female chickens of the FY strain.
According to claim 1,
The breeder in step (b) is a breeder (FHHY strain) obtained by crossing male chickens of the FH strain and female chickens of the HY strain.
According to claim 1,
The breeder in step (b) is a breeder (HFFY strain) obtained by crossing male chickens of the HF strain and female chickens of the FY strain.
According to claim 1,
The breeder in step (b) is a breeder (HFHY strain) obtained by crossing male chickens of the HF strain and female chickens of the HY strain.
A breeder produced by the method of any one of claims 1 to 9.

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