KR20220160170A - Composition for improving memory and cognitive ability, comprising Salvia officinalis L. extract and Hypericum perforatum L. extract - Google Patents

Abstract

The present invention relates to a composition containing a Salvia officinalis extract and a Hypericum perforatum extract, wherein the composition increases antioxidant, anti-inflammatory, brain nerve cell protection and acetylcholinesterase inhibitory effects, it is effective in improving memory and enhancing cognitive function. In addition, as a natural food and drug material, the composition can be consumed as actual food and has no side effects, and novel functional substances can be usefully used by utilizing agricultural biological resources.

Description

Composition for improving memory and cognitive ability, comprising Salvia officinalis L. extract and Hypericum perforatum L. extract}

The present invention relates to a composition for improving memory and/or cognitive function, and specifically relates to a composition for improving memory and/or cognitive function comprising a sage extract and a horseradish extract.

Dementia is a complex clinical syndrome that generally shows clear impairment in human cognitive function, intellectual ability, emotional and behavioral changes, etc. It is a state of great difficulty. Dementia is defined as a case of one or more impairments among other cognitive functions, including memory.

Dementia can be caused by various causes such as degenerative brain diseases such as Alzheimer's or Parkinson's disease, cerebral hemorrhage, metabolic diseases such as hepatic encephalopathy, Wilson's disease, infectious diseases such as neurosyphilis, acquired immunodeficiency syndrome, drug addiction such as alcohol, and brain trauma. In some forms, diseases that can lead to structural and functional abnormalities in the central nervous system can lead to dementia. Among them, dementia caused by Alzheimer's disease is the most common at 50 to 60%, followed by dementia caused by cerebrovascular disease.

Memory and cognitive impairment is the first and most common symptom experienced by people with Alzheimer's disease. In the early stages of Alzheimer's disease, patients suffer from recent memory impairment, which prevents them from remembering details of recent conversations or work. . However, during this time, past memories of events in the distant past are relatively well maintained. However, as the disease progresses, the cerebral cortex involved in the storage of long-term memories is damaged, and these memories of the past gradually become impaired.

Currently, in the case of Alzheimer's disease treatment in Korea, foreign companies are heavily dependent on imports, with 98% of the market share. Moreover, one company has 80% of the market, so it can be seen as a virtual monopoly. This shows that the growth of the dementia treatment field is urgent in terms of national competitiveness. Therefore, the development of effective anti-amnesia agents and memory improvers with few side effects can significantly help improve the quality of life and improve and treat early symptoms of Alzheimer's disease patients, and also help patients with amnesia and dementia who suffer from memory and cognitive impairment. It is believed that it can give

On the other hand, sage and pepper sprouts are industrially known as functional raw materials for health functional foods or pharmaceutical raw materials. In particular, prior art for sage for the treatment of muscle diseases and red pepper herb for antidiabetic use is known, but the effects of these two plants on the nervous system are unknown.

Registered Patent Publication No. 0626336 Patent Publication No. 10-2020-0104749

Under the above background, the present invention was completed by confirming that sage and western pepper herbs are excellent in improving memory and enhancing cognitive function.

Therefore, the present invention is to provide a composition for improving memory or cognitive function comprising a sage extract and a western pepper extract.

In order to achieve the above object, the present invention provides a composition for improving memory or enhancing cognitive function comprising a sage extract and a western pepper herb extract.

In addition, an anti-inflammatory composition comprising a sage extract and a horseradish extract is provided.

In addition, an antioxidant composition comprising a sage extract and a horseradish extract is provided.

The sage (Salvia officinalis L.) extract may be extracted from sage flowers, leaves, stems, roots, etc., and may preferably be an extract extracted from sage leaves, but is not limited thereto.

The western pepper herb (Hypericum perforatum L.) extract may be extracted from flowers, leaves, fruits, roots, stems, etc. of western pepper herbs, preferably an extract extracted from flowers, leaves, and fruits of western pepper herbs. It can be, but is not limited thereto.

The sage or capsicum herb in the present invention may be in an undried state, a dried state, or a dried and then pulverized state, but is not limited thereto.

The sage ( Salvia officinalis ) extract or western pepper herb ( Hypericum perforatum L. ) extract may be extracted using a polar solvent or organic solvent such as water or ethanol, but may be preferably extracted using water or ethanol, , More preferably, 50 to 90% (v / v) of fermented alcohol, more preferably 60 to 80% (v / v) of fermented alcohol can be used for extraction, but is not limited thereto.

In addition, the sage extract and the western pepper herb extract may be included in a weight ratio of 1: 1 to 5, preferably 1: 1 to 3, and more preferably 1: 1, but are not limited thereto.

The sage extract and the western pepper herb extract may be extracted at 40 to 80°C, preferably 50 to 70°C, more preferably 60°C for 1 to 3 hours, preferably 2 hours. When extracted below the temperature and time, the active ingredient may not be well extracted, and when extracted above the temperature and time, the active ingredient may be destroyed.

In the present invention, the term "memory" or "memory" refers to the ability to acquire new information, learned experience, or knowledge obtained from the surrounding environment, encode and store them in a specific part of the brain, and recall them again. The composition of the present invention has the effect of improving memory as described above, and this effect may include all effects of restoring damaged memory or memory impairment as well as the effect of enhancing memory of normal people. In addition, the memory impairment refers to a state in which the memory is lowered than that of a normal person due to various causes such as trauma, attention deficit, aging, disease, etc. It is a concept that includes

In the present invention, "cognitive function" is the ability to efficiently manipulate knowledge and information, and for the purpose of the present invention, cognitive dysfunction refers to a condition caused by a decrease in cognitive function, a disease resulting therefrom, and all diseases having such a symptom. As a concept that can be included, it may preferably mean a disorder caused by damage to brain nerve cells. Cognitive dysfunction of the present invention may include, but is not limited to, amnesia, Alzheimer's disease, vascular dementia, dementia due to head injury, or Parkinson's disease.

The composition for improving memory or enhancing cognitive function may be any one of a pharmaceutical composition or a food composition.

The anti-inflammatory composition may be a pharmaceutical composition for preventing, improving or treating inflammation or a cosmetic composition for preventing or improving inflammation or a food composition for preventing or improving inflammation.

Inflammatory disease in the present invention is atopic dermatitis, acne, psoriasis, sinusitis, rhinitis, conjunctivitis, asthma, dermatitis, glomerulonephritis, encephalitis, inflammatory enteritis, irritable bowel syndrome, chronic obstructive pulmonary disease, sepsis, septic shock, virus or Chronic inflammatory diseases caused by bacterial infections may be included, but are not limited thereto.

The antioxidant composition may be a food composition for antioxidant or a cosmetic composition for antioxidant.

The antioxidant may mean any action that prevents oxidation. Specifically, it may include reactive oxygen species (ROS) removal, ABTS and DPPH free radical scavenging, nitric oxide production inhibition, etc., but is not limited thereto.

The pharmaceutical composition of the present invention may contain the sage extract and the horseradish extract at a concentration of 10 ug/mg or more, preferably 100 ug/mg or more, more preferably 200 ug/mg or more, and still more preferably 400 ug/mg or more. It may be, but is not limited thereto.

In addition, the pharmaceutical composition may further include a pharmaceutically acceptable carrier, binder, lubricant, disintegrant, excipient, solubilizer, dispersant, stabilizer, suspending agent, etc., for example, lactose, dex Trose, Sucrose, Sorbitol, Mannitol, Xylitol, Erythritol, Maltitol, Starch, Alginate, Calcium Phosphate, Calcium Silicate, Maltodextrin, Silicon Dioxide, Cellulose, Methyl Cellulose, Microcrystalline Cellulose, Polyvinylpyrrolidone, Methylhydroxy It may be benzoate, propylhydroxybenzoate, talc, magnesium stearate, etc., but is not limited thereto.

In addition, the pharmaceutical composition may be prepared in a formulation such as a tablet, capsule, sustained-release preparation, elixir, suspension, syrup, wafer, unit dose injection ampoule, suspension, etc. by a method commonly used for formulation in the art.

The administration method of the pharmaceutical composition is oral administration, intravenous administration, intramuscular administration, intraarterial administration, intramedullary administration, intrathecal administration, intracardiac administration, transdermal administration, subcutaneous administration, intraperitoneal administration, intranasal administration, enteral administration , It may be administered by methods such as topical administration, rectal administration, topical administration, dermal administration, mucosal administration, intravertebral administration, including oral and sublingual administration, preferably oral administration, but is not limited thereto. In addition, at the time of administration, it may be formulated and administered in a formulation suitable for each administration method, and the formulation may be formulated by a method widely known in the art.

The pharmaceutical composition may be appropriately selected according to the age, weight, health condition, sex, administration time, administration route, excretion rate, active ingredient content, severity of a specific disease to be prevented or treated, etc., and the number of administrations is 1 It may be administered once or twice a day, every other day, every other week, or every other month.

The cosmetic composition includes components commonly used in cosmetic compositions, and may additionally include conventional adjuvants such as solvents, stabilizers, solubilizers, emulsifiers, vitamins, and fragrances, carriers, and the like.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art. For example, it may be manufactured in formulations such as lotion, nutrient lotion, nutrient cream, nutrient essence, pack, foundation, body oil, hair oil, shampoo, rinse, etc., but is not limited thereto.

The food composition may additionally include flavoring agents, flavoring agents, stabilizers, thickeners, preservatives, etc., which are commonly used in the art to prepare food, for example, lactose, dextrose, sucrose, Sorbitol, Mannitol, Xylitol, Erythritol, Maltitol, Starch, Alginate, Calcium Phosphate, Calcium Silicate, Maltodextrin, Silicon Dioxide, Cellulose, Methyl Cellulose, Microcrystalline Cellulose, Polyvinylpyrrolidone, Methylhydroxybenzoate, Propylhydroxy It may be benzoate, talc, magnesium stearate, etc., but is not limited thereto.

The food composition may be prepared in the form of beverages, gum, tea, vitamin complexes, powders, granules, tablets, capsules, confectionery, and the like.

The food composition may be appropriately selected according to the age, weight, health condition, sex, administration time, content of the active ingredient, severity of the disease, etc. of the subject to be consumed, and the number of intakes is once or twice a day, every other day, You can take it every other week, every other month, etc.

As the composition containing the sage extract and the horseradish extract of the present invention enhances antioxidant, anti-inflammatory (NO inhibition, IL-6 inhibition, TNF-alpha inhibition), brain nerve cell protection, and acetylcholinesterase inhibitory effects, It is effective in improving memory and enhancing cognitive function. In addition, as a natural food and drug material, it can be consumed as actual food and has no side effects, and new functional substances can be usefully used by utilizing agricultural biological resources.

Figure 1 shows the total phenol content increase and synergistic effect results of the composite extract of the present invention.
Figure 2 shows the results of confirming the brain nerve cell protection and synergistic effect of the composite extract (sage + western pepper herb) on the induction of scopolamine treatment death.
Figure 3 shows the results of the brain nerve cell protection and synergistic effect of the composite extract (sage + western pepper herb) for the induction of death by H ₂ O ₂ treatment.
Figure 4 shows the results of confirming the increase in the acetylcholinesterase inhibitory effect and the synergistic effect of the composite extract (sage + western pepper herb).

Hereinafter, the present invention will be described in detail by examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following Examples and Experimental Examples.

<Example 1> Preparation of sage and capsicum herb complex extract

After drying, 140 mL of each of 10 g of pulverized sage leaves and flowers, leaves, and fruits of horseradish was added using 70% (v/v) fermented alcohol as a solvent, extracted at 60 ° C for 2 hours, and then heated at 760 g. The supernatant centrifuged for 20 minutes was filtered with 6 μm filter paper. Thereafter, each extract was prepared by powdering after concentration under reduced pressure and lyophilization. Thereafter, the combination of sage extract and capsicum extract was used in the experiment by mixing the sage extract and the western pepper herb extract in an equal amount ratio (weight ratio) of 1:1 so that they could act synergistically and complement each other.

<Experimental Example 1> Antioxidant activity evaluation: total phenol content (TPC) evaluation

Total phenolic compound content was quantified colorimetrically by the Folin-Denis method. Add 1 mL of 0.2M phenol reagent to 1 mL of the centrifuged sample, leave it at room temperature for 3 minutes, add 1 mL of 2% Na ₂ CO ₃ solution, leave it at room temperature for 1 hour, and then use a UV spectrophotometer to measure 750 nm The absorbance was measured and the total phenol was converted into the amount of gallic acid from the standard curve prepared by the above method using gallic acid as a standard material. As a result of measuring the total phenol content for 10 mg/ml of the extract, the total amount of total phenol content in the combined extract (598μg/ml, sage + red pepper herb) compared to each single extract (sage 530μg/ml, horseradish herb 446μg/ml) As the phenol content increased, the synergistic effect of the composite extract was confirmed (FIG. 1).

<Experimental Example 2> Memory/cognitive ability improvement evaluation of the present invention

2-1. Brain nerve cell protective effect (MTT)

Human-derived SH-SY5Y neurons used in this experiment were purchased from the Korea Cell Line Bank. SH-SY5Y neurons were cultured at 37°C and 5% CO ₂ using Dulbecco's modified Eagle's medium (Gibco BRL., Gaithersburg, MD, USA) containing 10% fetal bovine serum, 100 U/mL penicillin and streptomycin. did In order to examine the protective effect of the extract against neuronal cell death, SH-SY5Y cells were equally divided into 6×10 ⁴ cells/well in a 96-well plate, cultured for 24 hours, and samples were treated according to each concentration. After 1 hour, 5 mM scopolamine or 150 μM H ₂ O ₂ , which induces neuronal cell death, was added and cultured for 24 hours. Thereafter, 10 μl of 5 mg/ml MTT was dispensed into each well and incubated in a 37° C. incubator without exposure to light for 3 hours. After removing the supernatant, adding 50 μl of DMSO and reacting at 37 ° C. for 10 minutes, absorbance was measured at 570 nm. The measured formazan production level was expressed as a percentage (%) compared to the measured value in normal cells.

As a result of measuring the protective effect on brain nerve cells by treating with 50 μg/ml of the extract and 5 mM of scopolamine, the combined extract (Sage + Western red pepper herb) showed excellent brain nerve cell protection and complex extract compared to each single extract (Sage, Western red pepper herb) The excellent synergistic effect of was confirmed (FIG. 2).

In addition, as a result of measuring the protective effect of brain nerve cells by treating with 50 μg/ml of the extract and 150 μM of H ₂ O ₂ , the brain nerve cells were excellent in the composite extract (sage + Western pepper herb) compared to each single extract (sage, western red pepper herb) It was confirmed that the excellent synergistic effect of protection and composite extract (Fig. 3).

2-2. Acetylcholinesterase inhibitory effect

Acetylcholinesterase is known as an enzyme that inhibits neurotransmission by destroying acetylcholine. In order to determine whether the extract inhibits the production of acetylcholinesterase, it was measured using an Acetylcholinesterase ELISA kit (CUSABIO). SH-SY5Y neurons were equally divided into 4×10 ⁵ cells/well in a 24-well plate, cultured for 24 hours, and samples were treated according to each concentration. After 1 hour, 5 mM of scopolamine, which induces neuronal cell death, was added and cultured for 24 hours, and then the culture supernatant was separated. The cell supernatant was diluted 10-fold, and the standard was diluted 2-fold to make standards 0 to 7. 100 μl of the diluted sample and standard were put into each well and incubated at 37°C for 2 hours, then the well liquid was removed and incubated with 100 μl of biotin-antibody at 37°C for 1 hour. After washing with wash buffer to remove unbound antibodies, 100 μl of HRP-avidin was added and incubated at 37°C for 1 hour. After washing, TMB substrate was added, incubated at 37°C for 30 minutes, stop solution was added, and 450nm The absorbance was measured at. Conversion into acetylcholinesterase concentration according to absorbance was converted into a formula according to the standard value.

As a result of measuring the concentration of acetylcholinesterase in the cell culture supernatant treated with 50 μg/ml of the extract and 5 mM of scopolamine, the acetylcholinesterase concentration in the composite extract (Sage + Western red pepper herb) was higher than that of each single extract (Sage and Western red pepper herb). It was confirmed that the cholinesterase inhibitory effect increased and the excellent synergistic effect of the complex extract (FIG. 4).

Claims (8)

A composition for improving memory or cognitive ability comprising a sage extract and a horseradish extract. The composition for improving memory or cognitive ability according to claim 1, wherein the sage extract and the western pepper herb extract are extracted with 50 to 90% (v/v) fermented alcohol. The composition for improving memory or cognitive ability according to claim 1, wherein the composition comprises sage extract: western pepper herb extract in a weight ratio of 1:1 to 3. The composition for improving memory or cognitive ability according to claim 1, wherein the sage extract is extracted from sage leaves. The composition for improving memory or cognitive ability according to claim 1, wherein the capsicum herb extract is extracted from flowers, leaves or fruits of capsicum. The composition for improving memory or cognitive ability according to claim 1, wherein the sage extract and the western pepper extract are extracted at 50 to 70°C. The composition for improving memory or cognitive ability according to claim 1, wherein the sage extract and the western pepper herb extract are extracted for 1 to 3 hours. The composition for improving memory or cognitive ability according to claim 1, wherein the composition is any one of a pharmaceutical composition and a food composition.

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