KR20220088469A - 세포-함유 유체의 전기천공을 위한 시스템, 방법 및 장치 - Google Patents

세포-함유 유체의 전기천공을 위한 시스템, 방법 및 장치 Download PDF

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KR20220088469A
KR20220088469A KR1020227017419A KR20227017419A KR20220088469A KR 20220088469 A KR20220088469 A KR 20220088469A KR 1020227017419 A KR1020227017419 A KR 1020227017419A KR 20227017419 A KR20227017419 A KR 20227017419A KR 20220088469 A KR20220088469 A KR 20220088469A
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South Korea
Prior art keywords
electroporation
chamber
electrode
cartridge
volume
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Pending
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Korean (ko)
Inventor
스티븐 후에이 여
시우 위 혼
한 웨이
이본 펙
웨이 상 리
벤용 시
치 와이 찬
벵 헹 림
다나라즈 삼무감
스테이시 테오
준 얀 탐
콕 셩 총
리양 림
마이클 고든
넥타리아 앤드로니코우
크리스 로스만
몰레라트 두 제우 사비에르 데
울리히 포케
Original Assignee
라이프 테크놀로지스 코포레이션
라이프 테크놀로지스 홀딩스 프리베이트 리미티드
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Publication of KR20220088469A publication Critical patent/KR20220088469A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
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  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
KR1020227017419A 2019-10-25 2020-10-23 세포-함유 유체의 전기천공을 위한 시스템, 방법 및 장치 Pending KR20220088469A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926078P 2019-10-25 2019-10-25
US62/926,078 2019-10-25
PCT/US2020/057138 WO2021081381A1 (en) 2019-10-25 2020-10-23 Systems and devices for electroporation of cell-containing fluid

Publications (1)

Publication Number Publication Date
KR20220088469A true KR20220088469A (ko) 2022-06-27

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KR1020227017419A Pending KR20220088469A (ko) 2019-10-25 2020-10-23 세포-함유 유체의 전기천공을 위한 시스템, 방법 및 장치

Country Status (10)

Country Link
US (2) US20210123009A1 (https=)
EP (1) EP4048776A1 (https=)
JP (2) JP7784374B2 (https=)
KR (1) KR20220088469A (https=)
CN (2) CN214361426U (https=)
AU (1) AU2020370481B2 (https=)
BR (1) BR112022007622A2 (https=)
CA (1) CA3154506A1 (https=)
DE (1) DE202020106067U1 (https=)
WO (1) WO2021081381A1 (https=)

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EP4048776A1 (en) * 2019-10-25 2022-08-31 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid
USD943760S1 (en) * 2020-05-11 2022-02-15 Maxcyte, Inc. Electroporation processing assembly
USD944410S1 (en) 2020-05-11 2022-02-22 Maxcyte, Inc. Electroporation processing assembly
USD965170S1 (en) * 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
US20230110090A1 (en) * 2021-10-08 2023-04-13 Life Technologies Holdings Pte Limited System and Method of Electroporation Protocol Optimization
TW202400835A (zh) 2022-03-09 2024-01-01 荷蘭商Ａｓｍ Ｉｐ私人控股有限公司 反應器系統、控制液體源的溫度之方法、及經調適以容納容器的裝置
WO2023230253A1 (en) * 2022-05-27 2023-11-30 Sqz Biotechnologies Company Cartridges and devices for use in a system for delivery of a payload into a cell
CN117431158A (zh) * 2022-07-14 2024-01-23 苏州壹达生物科技有限公司 一种流式电穿孔装置
US20260092248A1 (en) 2022-09-15 2026-04-02 Life Technologies Corporation Bioreactors operable in static and dynamic modes and methods of use
CN115722292B (zh) * 2022-11-22 2023-12-29 山东省煤田地质局第一勘探队 一种地下水自然资源调查用分类收集装置及使用方法
CN120882852A (zh) * 2023-06-21 2025-10-31 隆萨科隆有限公司 多腔室电穿孔盒及其使用方法
WO2026043806A2 (en) 2024-08-19 2026-02-26 Life Technologies Corporation Systems, methods, and assemblies for cell processing
CN120028438B (zh) * 2025-04-23 2025-08-26 晋城市鑫环球铸造有限公司 一种基于球墨铸铁管生产的探伤检测设备及方法

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Publication number Publication date
AU2020370481A1 (en) 2022-05-26
WO2021081381A1 (en) 2021-04-29
JP2026040486A (ja) 2026-03-09
CA3154506A1 (en) 2021-04-29
US20210123009A1 (en) 2021-04-29
EP4048776A1 (en) 2022-08-31
AU2020370481B2 (en) 2026-04-02
CN214361426U (zh) 2021-10-08
JP2023501142A (ja) 2023-01-18
DE202020106067U1 (de) 2020-12-04
JP7784374B2 (ja) 2025-12-11
BR112022007622A2 (pt) 2022-10-11
US20240263124A1 (en) 2024-08-08
CN112708552A (zh) 2021-04-27

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