KR20210149060A - Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합 - Google Patents
Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합 Download PDFInfo
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- KR20210149060A KR20210149060A KR1020217032103A KR20217032103A KR20210149060A KR 20210149060 A KR20210149060 A KR 20210149060A KR 1020217032103 A KR1020217032103 A KR 1020217032103A KR 20217032103 A KR20217032103 A KR 20217032103A KR 20210149060 A KR20210149060 A KR 20210149060A
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- transposon
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
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- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Physiology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (19)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962815187P | 2019-03-07 | 2019-03-07 | |
| US62/815,187 | 2019-03-07 | ||
| US201962822544P | 2019-03-22 | 2019-03-22 | |
| US62/822,544 | 2019-03-22 | ||
| US201962845218P | 2019-05-08 | 2019-05-08 | |
| US62/845,218 | 2019-05-08 | ||
| US201962855814P | 2019-05-31 | 2019-05-31 | |
| US62/855,814 | 2019-05-31 | ||
| US201962866270P | 2019-06-25 | 2019-06-25 | |
| US62/866,270 | 2019-06-25 | ||
| US201962873455P | 2019-07-12 | 2019-07-12 | |
| US62/873,455 | 2019-07-12 | ||
| US201962875772P | 2019-07-18 | 2019-07-18 | |
| US62/875,772 | 2019-07-18 | ||
| US201962884600P | 2019-08-08 | 2019-08-08 | |
| US62/884,600 | 2019-08-08 | ||
| US201962902171P | 2019-09-18 | 2019-09-18 | |
| US62/902,171 | 2019-09-18 | ||
| PCT/US2020/021568 WO2020181264A1 (en) | 2019-03-07 | 2020-03-06 | Rna-guided dna integration using tn7-like transposons |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20210149060A true KR20210149060A (ko) | 2021-12-08 |
Family
ID=72334850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217032103A Pending KR20210149060A (ko) | 2019-03-07 | 2020-03-06 | Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합 |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US10947534B2 (https=) |
| EP (1) | EP3935179A4 (https=) |
| JP (1) | JP7733576B2 (https=) |
| KR (1) | KR20210149060A (https=) |
| CN (1) | CN113795587A (https=) |
| AU (1) | AU2020232850B2 (https=) |
| BR (1) | BR112021017655A2 (https=) |
| CA (1) | CA3132197A1 (https=) |
| IL (1) | IL286089A (https=) |
| SG (1) | SG11202109550TA (https=) |
| WO (1) | WO2020181264A1 (https=) |
| ZA (1) | ZA202106516B (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20240055211A (ko) * | 2022-10-19 | 2024-04-29 | 성균관대학교산학협력단 | Tn7-유사 타입 CrisprCAS를 이용한 코리네박테리움 글루타미쿰(Corynebacterium glutamicum)균 유전체 내 유전자 삽입 시스템 |
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| WO2019090175A1 (en) | 2017-11-02 | 2019-05-09 | Arbor Biotechnologies, Inc. | Novel crispr-associated transposon systems and components |
| US20220372521A1 (en) * | 2019-08-14 | 2022-11-24 | The Trustees Of Columbia University In The City Of New York | Rna-guided dna integration and modification |
| AU2020373064A1 (en) * | 2019-11-01 | 2022-05-26 | Massachusetts Institute Of Technology | Type I-B CRISPR-associated transposase systems |
| CA3171941A1 (en) * | 2020-03-16 | 2021-09-23 | Joseph E. Peters | Compositions and methods comprising improved guide rnas |
| WO2021195532A1 (en) * | 2020-03-27 | 2021-09-30 | The Trustees Of Columbia University In The City Of New York | Genome engineering using crispr rna-guided integrases |
| US20240287486A1 (en) * | 2020-04-24 | 2024-08-29 | University Of Massachusetts | Improved cas 12a/nls mediated therapeutic gene editing platforms |
| US20250032629A1 (en) * | 2020-05-19 | 2025-01-30 | Anygen Co., Ltd. | Novel nucleolin-binding peptide and use thereof |
| CN116323971A (zh) * | 2020-08-18 | 2023-06-23 | Illumina公司 | 核酸的序列特异性靶向转座和选择以及分选 |
| EP4217499A4 (en) * | 2020-09-24 | 2025-02-12 | Metagenomi, Inc. | Systems and methods for transposing cargo nucleotide sequences |
| CA3194645A1 (en) * | 2020-10-08 | 2022-04-14 | The Broad Institute, Inc. | Type i crispr-associated transposase systems |
| WO2022147321A1 (en) * | 2020-12-30 | 2022-07-07 | The Broad Institute, Inc. | Type i-b crispr-associated transposase systems |
| WO2022150651A1 (en) * | 2021-01-07 | 2022-07-14 | The Broad Institute, Inc. | Dna nuclease guided transposase compositions and methods of use thereof |
| WO2022162623A1 (en) * | 2021-01-28 | 2022-08-04 | Arbor Biotechnologies, Inc. | Crispr-associated transposon systems and methods of using same |
| US20240301445A1 (en) * | 2021-01-28 | 2024-09-12 | Arbor Biotechnologies, Inc. | Crispr-associated transposon systems and methods of using same |
| CN112852921B (zh) * | 2021-03-16 | 2023-06-20 | 中国科学院长春应用化学研究所 | 一种基于即时检测试纸条的核酸检测方法、检测探针及其试剂盒 |
| CN115369098A (zh) * | 2021-05-17 | 2022-11-22 | 中国科学院分子植物科学卓越创新中心 | 一种新型crispr相关转座酶 |
| IL309148A (en) * | 2021-06-07 | 2024-02-01 | Univ Columbia | Crispr-transposon systems for dna modification |
| CA3223080A1 (en) * | 2021-06-17 | 2022-12-22 | Samuel Henry Sternberg | Systems, methods, and components for rna-guided effector recruitment |
| US20230159914A1 (en) * | 2021-06-24 | 2023-05-25 | Digenomix Corporation | Methods for reconstructing single cell genome |
| WO2023004422A2 (en) * | 2021-07-22 | 2023-01-26 | Arbor Biotechnologies, Inc. | Crispr-associated transposon systems and methods of using same |
| US20250066819A1 (en) * | 2021-08-16 | 2025-02-27 | Board Of Regents, The University Of Texas System | Crispr-associated transposons and uses thereof |
| EP4399312A4 (en) * | 2021-09-08 | 2025-09-03 | Metagenomi Inc | SYSTEMS AND METHODS FOR TRANSPOSITION OF CHARGED NUCLEOTIDE SEQUENCES |
| TWI821883B (zh) * | 2022-01-26 | 2023-11-11 | 國立清華大學 | 大腸桿菌基因編輯系統及其基因編輯方法 |
| US20250197458A1 (en) * | 2022-02-09 | 2025-06-19 | Cornell University | Adaptations for high efficiency i-f3-crispr-cas systems for guide rna-directed transposition in human cells |
| CA3244789A1 (en) * | 2022-02-23 | 2023-08-31 | Metagenomi Therapeutics, Inc. | FUSE PROTEINS |
| MX2024010333A (es) * | 2022-02-23 | 2024-08-30 | Metagenomi Inc | Sistemas y metodos para transponer secuencias de nucleotidos de carga. |
| CN114457100B (zh) * | 2022-03-03 | 2023-06-09 | 江南大学 | 一种基于CRISPR/Cpf1的大肠杆菌基因编辑系统及其应用 |
| US20250237648A1 (en) * | 2022-04-13 | 2025-07-24 | Baylor College Of Medicine | Bacterial biosensors for diagnosing and treating inflammation |
| WO2023204594A1 (ko) * | 2022-04-19 | 2023-10-26 | 서울대학교산학협력단 | 태그멘테이션을 이용한 벡터 삽입위치 검출 및 클론 정량 방법 |
| WO2023205267A1 (en) * | 2022-04-20 | 2023-10-26 | The Administrators Of The Tulane Educational Fund | Enhanced cell-free bacteriophage synthesis by genetic modulation of bacterial transcription/translation machinery (txtl) machinery |
| US20250290093A1 (en) * | 2022-04-29 | 2025-09-18 | Cz Biohub Sf, Llc | High capacity lentiviral vectors |
| EP4536817A1 (en) | 2022-06-07 | 2025-04-16 | Institut National de la Recherche pour l'Agriculture, l'Alimentation et l'Environnement | Attp mv4-derived site-specific recombination and its use for integration of sequence of interest |
| EP4536834A2 (en) * | 2022-06-08 | 2025-04-16 | North Carolina State University | Recombinant type i-f3 transposon-associated crispr-cas systems and methods of use |
| EP4569120A2 (en) * | 2022-06-13 | 2025-06-18 | The Trustees Of Columbia University In The City Of New York | Crispr-transposon systems for dna modification |
| CN118599809A (zh) * | 2022-07-07 | 2024-09-06 | 山东舜丰生物科技有限公司 | Cas酶及其应用 |
| JP2026500148A (ja) * | 2022-12-01 | 2026-01-06 | アーク リサーチ インスティテュート | 核酸操作のためのプログラム可能なdnaトランスポザーゼ |
| WO2024124048A1 (en) * | 2022-12-07 | 2024-06-13 | The Trustees Of Columbia University In The City Of New York | Systems and methods for rna-guided dna integration |
| CN116162640A (zh) * | 2023-01-30 | 2023-05-26 | 态创生物科技(广州)有限公司 | 大肠杆菌Rosetta菌株及其在催化合成α-熊果苷中的应用 |
| EP4665406A1 (en) * | 2023-02-14 | 2025-12-24 | The Trustees of Columbia University in the City of New York | Crispr-transposon systems and components |
| CN119842702B (zh) * | 2023-10-17 | 2025-11-28 | 清华大学 | 一种工程改造的dear核酸操纵系统的制备方法 |
| US20250257365A1 (en) * | 2024-02-09 | 2025-08-14 | California Institute Of Technology | Targeted DNA Integration in Plants by CRISPR-Associated Transposases (CASTs) |
| CN118460583B (zh) * | 2024-07-15 | 2024-10-29 | 苏州左旋星生物科技有限公司 | 用于靶向大肠杆菌多基因位点编辑的载体及其应用 |
| CN119752847B (zh) * | 2024-12-26 | 2025-12-05 | 崖州湾国家实验室 | 一种高温耐受型的TnpB核酸酶在核酸检测及基因编辑中的应用 |
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-
2020
- 2020-03-06 CA CA3132197A patent/CA3132197A1/en active Pending
- 2020-03-06 SG SG11202109550TA patent/SG11202109550TA/en unknown
- 2020-03-06 EP EP20766573.8A patent/EP3935179A4/en active Pending
- 2020-03-06 BR BR112021017655A patent/BR112021017655A2/pt unknown
- 2020-03-06 AU AU2020232850A patent/AU2020232850B2/en active Active
- 2020-03-06 KR KR1020217032103A patent/KR20210149060A/ko active Pending
- 2020-03-06 JP JP2021552850A patent/JP7733576B2/ja active Active
- 2020-03-06 CN CN202080033551.1A patent/CN113795587A/zh active Pending
- 2020-03-06 WO PCT/US2020/021568 patent/WO2020181264A1/en not_active Ceased
- 2020-03-06 US US16/812,138 patent/US10947534B2/en active Active
- 2020-06-26 US US16/913,299 patent/US12331292B2/en active Active
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- 2025-05-08 US US19/202,829 patent/US20260109980A1/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20240055211A (ko) * | 2022-10-19 | 2024-04-29 | 성균관대학교산학협력단 | Tn7-유사 타입 CrisprCAS를 이용한 코리네박테리움 글루타미쿰(Corynebacterium glutamicum)균 유전체 내 유전자 삽입 시스템 |
Also Published As
| Publication number | Publication date |
|---|---|
| US10947534B2 (en) | 2021-03-16 |
| JP2022524037A (ja) | 2022-04-27 |
| ZA202106516B (en) | 2023-10-25 |
| CN113795587A (zh) | 2021-12-14 |
| BR112021017655A2 (pt) | 2021-11-16 |
| US20200283769A1 (en) | 2020-09-10 |
| WO2020181264A1 (en) | 2020-09-10 |
| US12331292B2 (en) | 2025-06-17 |
| EP3935179A1 (en) | 2022-01-12 |
| AU2020232850B2 (en) | 2026-03-05 |
| IL286089A (en) | 2021-10-31 |
| US20200325474A1 (en) | 2020-10-15 |
| JP7733576B2 (ja) | 2025-09-03 |
| SG11202109550TA (en) | 2021-09-29 |
| US20260109980A1 (en) | 2026-04-23 |
| AU2020232850A1 (en) | 2021-10-07 |
| EP3935179A4 (en) | 2022-11-23 |
| CA3132197A1 (en) | 2020-09-10 |
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