KR20210049266A - Food compositions comprising probiotics lactic acid bacteria and a amino acid mineral complex as effective components - Google Patents

Abstract

The present invention relates to a food composition comprising probiotics lactic acid bacteria and an amino acid mineral complex as effective components, wherein the complex food composition does not inhibit the growth of lactic acid bacteria like prebiotics, rather, promotes growth and proliferation, has excellent bioabsorption rate, and does not impair the flavor of food. The composition also has excellent immune enhancing activity, thus can be used as health functional food for growing children.

Description

Food compositions comprising probiotics lactic acid bacteria and a amino acid mineral complex as effective components

The present invention relates to a food composition comprising probiotics lactic acid bacteria and amino acid mineral complex as active ingredients, and in particular, to a health functional food for children in the growing season.

Lactobacillus plays a role in making it an important nutrient component by decomposing fiber and complex proteins while coexisting in the human digestive system.In this way, it has a beneficial effect on the health of the host by improving the intestinal microbial environment of the host in the gastrointestinal tract of animals including humans. The state collectively refers to living microorganisms and is called probiotics.

In relation to probiotics for health functional foods in Korea, Dong Wha Pharm conducted research in 2016 to commercialize health functional foods for improving hyperglycemia of Lactobacillus Sake OK67 fermented products. As a result, Chong Kun Dang Bio Co., Ltd. has conducted research on the industrialization of anti-obesity probiotics, and A2Gen Co., Ltd. has conducted research on the development of cholesterol-lowering lactic acid bacteria materials. Meanwhile, CJ CheilJedang used the Lactobacillus Plantarum CJLP-133 strain of Patent No. 10-1406168 (the name of the invention “New Lactobacillus Plantarum and a composition using the same”), which was called “BYO Skin Lactobacillus” in 2015. We launched individually recognized health functional foods that can help in improvement.

On the other hand, the advantage of the amino acid mineral complex in mineral nutrition is the fact that it is easily absorbed by active transport or other mechanisms in mucosal cells and plant cells. That is, when minerals are absorbed by using amino acids as transport molecules, there is an advantage of avoiding the problem related to competition for active sites for absorption and the function of inhibiting absorption of special trace elements between minerals.

The mineral amino acid complex is generally produced by a reaction between an alpha-amino acid and a metal ion, and in order for the complex to have a ring structure, a metal ion having a valence of 2 or more is required. In this reaction, the positive charge of the metal ion is neutralized by reacting with the negative charge of the amino group or carboxyl group of the alpha-amino acid.

The structure, chemical formula, and bioavailability of the mineral amino acid complex are diverse, and representatively, Ashmead et al., Chelated Mineral Nutrition, (1982), Chas. C. Thomas Publishers, Springfield, Ill. ), Ashmead et al., Intestinal Absorption of Metal Ions, (1985)), Ashmead et al., Foliar Feeding of Plants with Amino Acid Chelates, (1986)) and U.S. Patent No. 4,020,158 , 4,167,564, 4,216,143, 4,721,644, 4,599,152, 4,774,089, 4,830,716, 4,863,898, 4,725,427, etc.

In the case of probiotic lactic acid bacteria notified in the'Standards and Standards of Health Functional Food' (Ministry of Food and Drug Safety Notice No. In the case of zinc, functional labels such as'Necessary for normal immune function' and'Necessary for normal cell division' are possible.

Minerals are essential for the growth of probiotics, but as a health functional food, there is a problem that if excessive amounts are used as a functional content, they may rather inhibit the growth of probiotics and lactic acid bacteria, and until now, the physiological activity of lactic acid bacteria or mineral amino acid complexes alone has been verified. There is one example, but no method has been reported to enhance their effectiveness.

U.S. Patent No. 4,020,158 U.S. Patent No. 4,167,564

Ashmead et al., Chelated Mineral Nutrition, (1982), Chas. C. Thomas Publishers, Springfield, Ill. Chas. C. Thomas Publishers, Springfield, Ill. Ashmead et al., Intestinal Absorption of Metal Ions, (1985) Ashmead et al., Foliar Feeding of Plants with Amino Acid Chelates, (1986)

The present invention applies a water-soluble amino acid mineral complex and lactic acid bacteria as a composition for enhancing immune activity, enhances the active absorption of minerals in the small intestine, improves the immunity of humans or animals other than humans, It is intended to provide a food, pharmaceutical or feed composition that can exhibit an effect.

The present invention, probiotics lactic acid bacteria; And molecular formula C ₄ H ₉ NO ₇ Zn, zinc aspartic acid hydrate of Formula 1, wherein 1 g of zinc aspartic acid hydrate has water solubility in 1 to 30 ml of water at 25° C., and is an orthorhombic crystal. It relates to a food composition for enhancing immune activity using a mineral complex as an active ingredient.

[Formula 1]

The probiotics are lactobacillus acidophilus ( L.acidophilus ), Lactobacillus casei ( L.casei ), Lactobacillus gasseri (L.gasseri), Lactobacillus delbruki subsphysis bulgaricus (L. delbrueckii ssp.bulgaricus ), Lactobacillus helveticus (L.helveticus), Lactobacillus fermentum (L.fermentum), Lactobacillus paracasei (L.paracasei), Lactobacillus plantarum ( L.plantarum ), Lactobacillus plantarum (L. Bacillus luteri (L.reuteri), Lactobacillus rhamnosus (L.rhamnosus) and Lactobacillus salivarius ( L.salivarius ) any one or more Lactobacillus lactic acid bacteria selected from; Lactococcus lactis ; Enterococcus Passage Titanium (E.faecium) and Enterococcus faecalis blood (E.faecalis) any one or more of Enterococcus genus lactobacillus is selected from; Streptococcus Thermo filler's (S.thermophilus); And Bifidobacterium Bifidobacterium bonus (B.bifidum), Bifidobacterium breve (B.breve), Bifidobacterium ronggeom (B.longum) and Bifidobacterium lactis Annie Marlies sub-speaker system (B. animalis spp. lactis ) any one or more selected from the Bifidobacterium genus lactic acid bacteria; may be any one or more lactic acid bacteria selected from the group consisting of.

The food composition for enhancing immune activity may further include organic passivated calcium and organic passivated magnesium.

The composition for enhancing immune activity may be a health functional food for children in the growing season.

The present invention relates to a food composition containing probiotics lactic acid bacteria and amino acid mineral complexes as active ingredients, and does not inhibit the growth of lactic acid bacteria like prebiotics, but rather promotes growth and proliferation, while having excellent bioabsorption rate and not inhibiting the flavor of food. As a complex food composition, since it has excellent immunity enhancing activity, it can be used as a health functional food for growing children.

Hereinafter, the present invention will be described in more detail.

The present invention, probiotics lactic acid bacteria; And molecular formula C ₄ H ₉ NO ₇ Zn, zinc aspartic acid hydrate of Formula 1, wherein 1 g of zinc aspartic acid hydrate has water solubility in 1 to 30 ml of water at 25° C., and is an orthorhombic crystal. It relates to a food composition for enhancing immune activity using a mineral complex as an active ingredient.

[Formula 1]

The food composition for enhancing immune activity is a single formulation formed by mixing the amino acid mineral complex of Formula 1 with probiotic lactic acid bacteria, which does not inhibit the growth of probiotic lactic acid bacteria, but rather promotes the growth and proliferation of prebiotic activity, and contains minerals in the small intestine. It is better absorbed and has the effect of enhancing the immune activity in the intestine.

The immunity enhancement refers to increasing the immune response or activity of the immune system in vivo, and is effective in alleviating the decrease in immune function due to aging, pregnancy, immunosuppressive treatment, etc., or in preventing or improving diseases caused by the decrease in immune function. And, the disease caused by the lowering of the immune function may be any one or more selected from virus-sensitive diseases and inflammatory diseases such as a cold, chronic fatigue, and cancer.

In the food composition for enhancing immune activity, the amino acid mineral complex of Formula 1 may be mixed to be 3.6 to 12 mg based on the daily intake of zinc, and the probiotic lactic acid bacteria are 1 × 10 ⁸ to 1 × 10 8 based on the daily intake. It can be mixed so as to be 1×10 ^10.

The food composition for enhancing immune activity may further include organic passivated calcium and organic passivated magnesium. Organic passivated calcium may be mixed to be 210 to 800 mg based on the daily intake of calcium, and the organic passivated magnesium may be mixed to be 66 to 250 mg based on the daily intake of magnesium.

The food composition for enhancing immune activity may be a health functional food for growing children. The term "children in the growing period" refers to children aged 3 to 15 years old.

The food composition for enhancing the immune activity is preferably to be ingested 1 to 3 times a day, and the dose per intake may be 100 mg to 5 g, preferably 500 mg to 2 g.

The probiotic lactic acid bacteria refer to beneficial bacteria living in the intestine of a host and a strain that has a beneficial effect on the intestinal environment when ingested and reached the intestine. These mean bacteria that survive and reach gastric acid and bile acids, multiply and settle in the intestine, exhibit useful effects in the intestine, are non-toxic, and satisfy non-pathogenic conditions.

The probiotics are lactobacillus acidophilus ( L.acidophilus ), Lactobacillus casei ( L.casei ), Lactobacillus gasseri (L.gasseri), Lactobacillus delbruki subsphysis bulgaricus (L. delbrueckii ssp.bulgaricus ), Lactobacillus helveticus (L.helveticus), Lactobacillus fermentum (L.fermentum), Lactobacillus paracasei (L.paracasei), Lactobacillus plantarum ( L.plantarum ), Lactobacillus plantarum (L. Bacillus luteri (L.reuteri), Lactobacillus rhamnosus (L.rhamnosus) and Lactobacillus salivarius ( L.salivarius ) any one or more Lactobacillus lactic acid bacteria selected from; Lactococcus lactis ; Enterococcus Passage Titanium (E.faecium) and Enterococcus faecalis blood (E.faecalis) any one or more of Enterococcus genus lactobacillus is selected from; Streptococcus Thermo filler's (S.thermophilus); And Bifidobacterium Bifidobacterium bonus (B.bifidum), Bifidobacterium breve (B.breve), Bifidobacterium ronggeom (B.longum) and Bifidobacterium lactis Annie Marlies sub-speaker system (B. animalis spp. lactis ) any one or more selected from Bifidobacterium lactic acid bacteria; may be any one or more selected from the group consisting of.

The amino acid mineral complex of Formula 1 is obtained by adding aspartic acid to water and stirring; In the aspartic acid aqueous solution obtained in the stirring step, zinc oxide is added to the zinc precursor so that the zinc precursor and aspartic acid have a ratio of 1: 0.8 to 2.5 moles, followed by heating at 50 to 100° C. for 10 minutes to 24 hours while stirring. step; And removing the insoluble matter after the step of reacting.

The zinc precursor is a zinc salt or zinc oxide that can be used as food, medicine or feed, and zinc oxide, which is insoluble in water, can be used, and zinc oxide has more excellent activity of increasing the antibody production rate of an antiviral vaccine. In particular, the use of a water-soluble zinc salt, which is well soluble in water, has been taken for granted in the manufacture of a conventional zinc aspartic acid complex, but a zinc aspartic acid complex that is more effective when heated for more than a certain temperature and time while using zinc oxide, which is insoluble in water, is produced. It was confirmed that it was formed.

The molar ratio of the zinc precursor and aspartic acid refers to the molar ratio of zinc and aspartic acid, and 1: 0.8 to 2.5 mol, preferably 1: 1.5 to 2.2 mol is advantageous. The theoretical molar ratio of zinc and aspartic acid in the zinc aspartic acid hydrate of Formula 1 is 1:2, but when aspartic acid is added in an excessive amount compared to the zinc precursor, the insoluble zinc oxide is reduced and water-soluble zinc aspartic acid hydrate is more easily formed. can do.

The step of reacting is preferably heated at 50 to 100° C. for 10 minutes to 24 hours, particularly, in the case of zinc oxide, at 80 to 100° C. for 1 hour or more, preferably 1.5 hours. On the other hand, zinc oxide is mostly about 95%, preferably 99% or more of aspartic acid reacts within 3 hours, so even if heated for more than 3 hours, the reduction of insolubles or unreacted materials may not be significant. In the reaction step, ultrasonic treatment may be used to shorten the reaction time.

It may further include the step of removing insoluble matter from the reaction solution of the above step. The step of removing the insoluble matter may be further required when zinc oxide is used as a zinc precursor.

The manufacturing method has the advantage that it does not require additional additives other than a zinc precursor, aspartic acid, and water, unlike a conventional method of manufacturing a zinc aspartic acid composite, and the manufacturing process is simple, thereby reducing process cost.

Probiotics lactic acid bacteria are mixed in the amino acid mineral complex. In this case, the probiotics lactic acid bacteria may be cultured in MRS, which is a lactic acid bacteria culture medium, at 30-40° C. for 12 hours.

The food composition for enhancing immune activity of the present invention has remarkably superior immune activity when using a mixture of amino acid mineral complex and lactic acid bacteria, rather than using probiotic lactic acid bacteria or amino acid mineral complex, respectively, and proliferation activity of beneficial bacteria in the intestine is more I was able to confirm that it was excellent.

In the present specification, "as an active ingredient" means containing an amount sufficient to achieve the efficacy or activity of the probiotic lactic acid bacteria and mineral amino acid complex.

The food composition according to the present invention can be used as a health functional food or added to various general foods. Examples of the general foods include beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, gum, ice cream, vitamin complexes, and health supplements.

The food composition of the present invention may include not only probiotic lactic acid bacteria and mineral amino acid complexes as active ingredients, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavoring agents. Includes. Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is made of drinks and beverages, it may further include citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts in addition to the probiotic lactic acid bacteria and mineral amino acid complex. .

The food composition of the present invention can be used for inhibiting the growth of beneficial bacteria in the intestine and the growth of harmful bacteria in the intestine. The intestinal beneficial bacteria refer to the existing beneficial bacteria living in the intestine of a host and a strain that has a beneficial effect on the intestinal environment when ingested and reached the intestine. Intestinal harmful bacteria refer to harmful bacteria living in the intestine of a host and a strain that acts harmful to the intestinal environment when ingested and reached the intestine.

The intestinal beneficial bacteria may be at least one strain selected from the genus Bifidobacterium, genus Leukonostock, genus Pediococcus, genus Weisella, genus Streptococcus, genus Lactobacillus, and combinations thereof.

The intestinal harmful bacteria may be at least one strain selected from Clostridium genus, Eubacterium genus, Bacteroid genus, E. coli genus, Bacillus genus, Salmonella genus, and combinations thereof. More preferably, the strain of the genus Clostridium is at least one selected from Clostridium difficile, Clostridium perfringens, and combinations thereof,

Hereinafter, the present invention will be described in more detail through examples and the like, but the scope and contents of the present invention are reduced or limited by the examples below and cannot be interpreted. In addition, if based on the disclosure of the present invention including the following examples, it is obvious that the present invention for which no specific experimental results are presented can be easily carried out by a person skilled in the art. It is natural to fall within the scope of the claims.

Preparation Example 1: Preparation of zinc aspartic acid complex

57.5 g of aspartic acid was added to 500 ml of water, and 17.5 g of zinc oxide (Zinc 81% by weight) was added thereto with stirring, reacted sufficiently at a temperature of 90° C. for at least 120 minutes, and the reaction solution was filtered through a 0.2 μm filter paper. Then, the filtrate was concentrated to 80% at 65° C. for 3 hours, and then dried in vacuo to obtain a water-soluble zinc aspartic acid complex.

Experimental Example 1: Confirmation of the structure of the zinc aspartic acid complex of Preparation Example 1

The structure of the zinc aspartic acid complex of Preparation Example 1 was confirmed using Bruker SMART APEX II X-ray Crystallograph (XRC) and Bruker SHELXTL Structure Analysis Program.

Crystallographic data

Molecular Formula C ₄ H ₉ NO ₇ Zn

Molecular Weight 248.49

Crystal system Orthorhombic

Space group P2(1)2(1)2(1)

Z 4

Unit cell dimension a=7.8012(15)Å α=90°

b=9.3435(17)Å β=90°

c=11.576(2)Å γ=90°

Volume 843.8(3) / Å ³

Calculated density 1.956Mg/m ³

Temperature 296(1)K

Absolute structural parameters 0.00

Crystal size 0.18 x 0.12 x 0.08 mm ³

F(000) 504

Absorption coefficient 2.920 mm ^-1

In addition, atomic coordinates of the zinc aspartic acid complex of Preparation Example 1 (x10 ⁴ ), equivalent isotropic displacement parameters (Å ² x 10 ³ ), binding length (Å), angle (°), anisotropic displacement _{Molecular formula C 4} H by checking the parameters (Å ² x10 ³ ), hydrogen coordinates (x10 ⁴ ), equivalent isotropic displacement parameters (Å ² x10 ³ ), and torsion angles (°). ₉ NO ₇ Zn, and it was confirmed that the zinc aspartic acid hydrate of the following formula (1)

[Formula 1]

Example 1: Preparation of a composition for enhancing immune activity of zinc aspartic acid complex, organo-passivated calcium, organo-passivated magnesium, Bifidobacterium bifidum, and Lactobacillus luteri

The zinc aspartic acid complex of Preparation Example 1 was mixed to be 6 mg/g based on zinc, and organic passivated calcium (calcium aspartic acid complex) manufactured by BTN Co., Ltd. was mixed to be 400 mg/g based on calcium. In addition, organic passivated magnesium (magnesium aspartic acid complex) was mixed to be 100 mg/g based on magnesium, and Bifidobacterium bifidum bacteria and Lactobacillus reuteri bacteria were each mixed in a culture medium for lactic acid bacteria. The food composition for enhancing the immune activity of Example 1 was prepared by mixing the phosphorus MRS at 37° C. for 12 hours and then ^{mixing each to 1×10 9 CFU/g.}

Example 2: Preparation of a composition for enhancing immune activity by mixing zinc aspartic acid complex, Bifidobacterium bifidum, and Lactobacillus luteri

The zinc aspartic acid complex of Preparation Example 1 was mixed so as to be 6 mg/g based on zinc, and Bifidobacterium bifidum bacteria and Lactobacillus reuteri bacteria were respectively added to MRS, which is a lactic acid bacteria culture medium. After cultivation at 37° C. for 12 hours, the food composition for enhancing immune activity of Example 2 was prepared by mixing ^{each to 1×10 9 CFU/g.}

Example 3: Preparation of a composition for enhancing immune activity by mixing zinc aspartic acid complex and Lactobacillus luteri

The zinc aspartic acid complex of Preparation Example 1 was mixed so as to be 6 mg/g based on zinc, and Lactobacillus reuteri bacteria were enriched for 12 hours at 37°C in MRS, a lactic acid bacteria culture medium, and then 1 × 10 ⁹ A food composition for enhancing immune activity of Example 3 was prepared by mixing so as to be CFU/g.

Comparative Example 1: Preparation of a composition for enhancing immune activity by mixing inorganic zinc, calcium and magnesium, Bifidobacterium bifidum, and Lactobacillus luteri

Zinc oxide, calcium lactate, and sulfuric acid, respectively, in place of the zinc aspartic acid complex of Preparation Example 1 used in Example 1, organo-passivated calcium (calcium aspartic acid complex) and organo-passivated magnesium (magnesium aspartic acid complex) manufactured by BTN Co., Ltd. Magnesium is used, and the amounts are mixed so that they are 6 mg/g, 400 mg/g and 100 mg/g, respectively, based on zinc, calcium and magnesium, and Bifidobacterium bifidum and Lactobacillus luteum are examples. The food composition of Comparative Example 1 was prepared by mixing in the same manner as in 1.

Comparative Example 2: Preparation of a composition for enhancing immune activity by mixing inorganic zinc and Lactobacillus luteri

Zinc oxide was used instead of the zinc aspartic acid complex of Preparation Example 1 used in Example 3, and the amount of each was mixed so as to be 6 mg/g based on zinc, and Lactobacillus lutein was mixed in the same manner as in Example 3. Thus, a food composition of Comparative Example 2 was prepared.

Experimental Example 1: Measurement of the amount of nitrogen oxide produced

In order to confirm the immunity enhancing effect of Examples 1 to 3 and Comparative Examples 1 and 2, the ability to produce nitric oxide was confirmed.

The NO production ability in Raw 264.7 cells priming with 50 ng/ml IFN-γ was confirmed by using the food compositions of Examples 1 to 3 and Comparative Examples 1 and 2 at a concentration of 50 ppm. The control was compared with the NO production ability by LPS 1 μg/ml. The RAW 264.7 cells were distributed from the Korea Cell Line Bank, and cultured in a 5% CO2, 30°C incubator using Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS and 1% Anti-anti as a culture medium.

The production of nitric oxide in the culture solution was evaluated through measurement of nitrous acid, an oxide of nitric oxide. Griess analysis method was used, and the cells of Raw 264.7 cells were dispensed in a 96 well plate at a ^{concentration of 2 x 10 5} cells/mL, cultured for 24 hours, and then prepared at 500 μg/mL in Raw 264.7 cells. Each was treated at a concentration of 50 μg/mL, and then incubated for 20 hours. After incubation, 100 μL of the supernatant was transferred to a new 96-well plate, mixed 1:1 with griess reagent (SigmaAldrich Co.), and reacted for 10 minutes in a shaker, and absorbance was measured at 540 nm using a microplate reader. The standard curve was measured using sodium nitrite, and the results are shown in Table 1.

division Control
(LPS) Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Nitric oxide (%) 100 142.7% 136.4% 130.8% 63.5% 52.3%

Based on the amount of nitrogen oxide produced by the positive control LPS, in the order of Example 1, Example 2, and Example 3, the amount of nitrogen oxide produced was significantly increased compared to the control group. Rather, it decreased.

Experimental Example 2: Confirmation of the effect on the growth activity of beneficial bacteria in the intestine

In order to examine the effect of the composition of the present invention on the growth activity of Bifidobacterium longum, Lactobacillus sp. and Lactobacillus acidophilus strains among beneficial bacteria in the intestine, the following experiment was performed. Experiments on the effect on the growth activity of beneficial bacteria in the intestine were repeated three times, and the experimental results are O.D. of the experimental groups treated with Examples 1 to 3 and Comparative Examples 1 and 2 for the control group treated with water. Expressed as a ratio to the value.

1) Preparation of intestinal bacteria

Bifidobacterium longum (ATCC 15707), Lactobacillus sp. (KCTC 3930), and Lactobacillus acidophilus (Lactobacillus acidophilus ATCC 4356) were used as indicators of intestinal beneficial bacteria. The strains were cultured under anaerobic conditions placed in BBL GasPak (Becton Dickinson and Company, USA) at 37°C in Reinforced Clostridial Media (RCM, Difco, USA) medium.

2) Growth activity experiment by agar diffusion method

After sterilizing the EG agar medium, it was cooled to 50°C, and three plates inoculated with the monarchs were made so that each of the three strains contained 2-3%, and the medium was solidified at room temperature. After 0.1 mg of the compositions of Examples 1-1, 1-2, 1-3, and 1-4 were respectively added dropwise to a 6 mm diameter disk paper (Whatman paper No.41), they were placed at regular intervals on the solidified medium. . The medium was cultured at 37° C. for 48 hours under anaerobic conditions, and then the size of the growth active ring was observed.

Strain Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Bifidobacterium long gum +++ +++ ++ - - Lactobacillus Esp ++ +++ ++ + + Lactobacillus Acidophilus ++ ++ ++ + -

As a result, as shown in Table 2, in the solid medium treated with Examples 1, 2, and 3, the Bifidobacterium longum strain exhibited a growth activity ring of about ++ to +++, but Comparative Example 1 and In 2, the growth activity ring of-degree was shown.

The Lactobacillus sp. strains treated with Examples 1, 2 and 3 exhibited a growth activity ring of about ++ to +++, but in Comparative Examples 1 and 2, a growth activity ring of about + was shown.

The Lactobacillus acidophilus strains treated with Examples 1, 2 and 3 showed a growth activity ring of about ++, but in Comparative Examples 1 and 2, a growth activity ring of about-to + was shown.

Overall, it was confirmed that the composition for enhancing immune activity of the present invention has a high growth activity effect against beneficial bacteria in the intestine.

Claims (4)

Probiotics lactic acid bacteria; And
Molecular formula C ₄ H ₉ NO ₇ Zn, zinc aspartic acid hydrate of the following formula (1), 1 g of zinc aspartic acid hydrate has water solubility in 1 to 30 ml of water at 25°C, and is an orthorhombic crystal amino acid mineral Complex;
Food composition for enhancing immune activity using as an active ingredient:
[Formula 1]

. The method of claim 1,
The probiotic lactic acid bacteria,
Lactobacillus ash FIG filler's (L.acidophilus), Lactobacillus casei (L.casei), Lactobacillus biasing Li (L.gasseri), Lactobacillus del brewer sub key RY cis Bulgaria kusu (L.delbrueckii ssp. Bulgaricus) , L.helveticus , L.fermentum , L.paracasei , L.plantarum , L. reuteri ), Lactobacillus rhamnosus (L.rhamnosus) and Lactobacillus salivarius ( L.salivarius ) any one or more Lactobacillus lactic acid bacteria selected from;
Lactococcus lactis ;
Enterococcus Passage Titanium (E.faecium) and Enterococcus faecalis blood (E.faecalis) any one or more of Enterococcus genus lactobacillus is selected from;
Streptococcus Thermo filler's (S.thermophilus); And
Bifidobacterium Bifidobacterium bonus (B.bifidum), Bifidobacterium breve (B.breve), Bifidobacterium ronggeom (B.longum) and Bifidobacterium lactis Annie Marlies sub-speaker system (B.animalis spp. lactis ) any one or more selected from among the lactic acid bacteria of the genus Bifidobacterium; food composition for enhancing immune activity, characterized in that it is any one or more lactic acid bacteria selected from the group consisting of. The method of claim 1,
The food composition for enhancing immune activity is a food composition for enhancing immune activity, characterized in that it further comprises organic passivated calcium and organic passivated magnesium. [4] The food composition for enhancing immune activity according to any one of claims 1 to 3, wherein the composition for enhancing immune activity is a health functional food for children in the growing season.