KR20210047061A - Production method of phellinus linteus culture medium - Google Patents

Abstract

A production method of a Phellinus linteus culture medium according to an embodiment of the present invention comprises the following steps of: a grain medium manufacturing step using water and grains selected from grains such as brown rice, roasted brown rice, and barley; preparing a liquid seed of Phellinus linteus; inoculating the prepared Phellinus linteus liquid seed in the prepared grain medium; culturing the inoculated grain medium; and inoculating the cultured medium into oak trees to cultivate the Phellinus linteus. The purpose of the present invention is to improve the cultivation efficiency of Phellinus linteus and furthermore, to promote the supply of Phellinus linteus, which is difficult to cultivate.

Description

Method for manufacturing Psoriasis mushroom medium{PRODUCTION METHOD OF PHELLINUS LINTEUS CULTURE MEDIUM}

The present invention relates to a method for producing a Pseudomonas mushroom medium, and more particularly, by culturing a grain medium containing immersed brown rice as a main component, and using a liquid seed mixture of brown rice, fructose, malt extract, soybean meal, distilled water, etc. The present invention relates to a method of manufacturing a Pseudomonas mushroom medium that facilitates cultivation and inoculation of Pseudomonas mushroom mycelium and shortens cultivation period.

Pseudomonas mushrooms are perennials and are distributed in Korea, China, and Japan as a wood-borne fungus that overlaps with mulberry trees. The dark brown hairs are short and dense on the surface, and as they grow, the hairs disappear and become keratinized. There are blackish brown ring grooves, and the edges are bright yellow, but the lower side is yellowish brown and the flesh is yellowish brown, so it is called a situation.

Pseudomonas mushroom is a rare basidiomycete perennial mushroom that does not reproduce well. The fruiting body of Pseudomonas mushrooms has been confirmed to be non-toxic when administered orally, and food is registered as a food ingredient in the Food Code. In addition, Pseudomonas mushrooms contain a large amount of mineral elements such as calcium, potassium, magnesium, vitamin B2 and vitamin C, and have been used as a treatment for uterine bleeding, genital tumors, and intestinal bleeding due to their hemostasis, bleeding, and harmonious action. .

On the other hand, Psoriasis mushrooms are known to have excellent anticancer effects and are used as valuable medicinal materials in Korea due to their wide range of applications, but artificial cultivation techniques are being attempted in various ways because the amount of natural Pseudomonas mushrooms is not collected and cultivated is not easy. .

As a prior art related to the cultivation technology of Pseudomonas mushrooms, Korean Utility Model No. 20-0270012 (Sakisuki mushroom cultivation apparatus) has a body with an open top and a lid for opening and closing the open top of the body, and the lid or body A housing in which one or more ventilation holes are formed at the top; A moisture-containing layer provided on the bottom of the housing and containing moisture; And a plurality of logs for cultivation of mushrooms planted in the moisture-containing layer of the housing. The moisture-containing layer is made of sand, sawdust, rice bran or mixtures thereof. In addition, it is characterized in that a moss layer is formed on the upper surface of the wood so that the humidity inside the housing can be visually checked.

In addition, Korean Laid-Open Patent Publication No. 10-2005-0076380 (a method of cultivating mushroom mushrooms and a cultivation device used therefor) is a mixture of mulberry sawdust and oak sawdust, or mulberry sawdust, oak sawdust and rice bran at a certain ratio, and Making a medium by adding moisture; Filling the medium into the culture vessel and forming a tunnel for inoculation in the medium; As a cap having an upper opening and a lower opening, a cap lid with an opening formed therein and a filler that allows air to flow through the opening is installed at the upper opening, and the internal capacity of the culture vessel and Installing a cap having substantially the same internal capacity on the culture vessel by using the lower end opening portion; Sterilizing the culture container in which the cap is installed, filling the inoculation tunnel in the sterilized culture container with the seeds of Pseudomonas mushrooms up to the top, and inoculating hyphae in the culture chamber; Moving the culture vessel in which the cap is installed to a cultivation company and sequentially repeating light culture, dark culture, and light culture; And performing the first harvesting after removing the cap, and then covering the cap again to repeat the harvesting by repeating light culture, dark culture, and light culture.

In addition, Korean Laid-Open Patent Publication No. 10-2015-0053357 (Situation mushroom cultivation method) includes the steps of preparing a solid wood medium, sterilizing the solid wood medium, inoculating the seed into the solid wood medium and culturing the mycelium, the mycelium It characterized in that it comprises the step of inserting a nail into the top of the cultured wood medium, the step of cultivating for 22 to 28 months by hanging the wood medium into which the nail is inserted.

However, the conventional techniques mentioned above are those having a technical configuration different from that of the present invention, and the method of inoculating the mycelium is different from that of the liquid spawn of the present invention, and the growth of the mycelium is much There is a downside to being slow.

Therefore, it is necessary to improve the efficiency of cultivation of Pseudomonas mushrooms and develop technology for cultivation of Pseudomonas mushrooms that consumers can safely consume.

The present invention is to improve the cultivation efficiency of Pseudomonas mushrooms, and further promote the supply of Psoriasis mushrooms, which are difficult to cultivate, by preparing Psoriasis mushrooms medium using liquid seed bacteria. There is a purpose.

A method for manufacturing a phylum mushroom culture medium according to an embodiment of the present invention for solving the above problem includes: a grain medium manufacturing step using grain and water selected from grains such as brown rice, roasted brown rice, and barley; Preparing a liquid mushroom spawn; Inoculating the prepared Pseudomonas mushroom liquid spawn on the prepared grain medium; Culturing the prepared grain medium; And inoculating the cultured medium to the tree It may include the step of cultivating the mushrooms.

In the grain medium manufacturing step, the brown rice and water are sterilized at a high temperature of 120° C. or higher for 1 to 2 hours, and then cooled to 24 to 27° C. to prepare a grain medium in a sterile state.

In the step of preparing the liquid seed, a culture obtained by mixing brown rice, fructose, malt extract, soybean meal, distilled water, and green plum extract was autoclaved at 115 to 135°C for 60 to 90 minutes, and then at 25 to 27°C. It can be cultured for 12 days to prepare a culture solution.

In addition, after inoculating the protozoa of Pseudomonas mushrooms in the culture solution, air may be injected at 25 to 27°C for 8 to 12 days to prepare a liquid seed.

In the step of inoculating the plant mushroom liquid seed, the prepared plant mushroom liquid seed can be inoculated into the prepared grain medium to cultivate the plant mushroom medium for 60 to 90 days in a clean room maintained at 24 to 27°C.

In the step of cultivating Pseudomonas mushrooms, Pseudomonas mushrooms may be cultivated by inoculating a medium in which Pseudomonas mushrooms are cultured on oak trees.

In addition, in the step of preparing the liquid seed, the green plum extract may be an extract obtained by hot water extraction by adding green plum fruit to a green plum mixture obtained by juicing the green plum juice and water.

In addition, the green plum extract may be extracted with hot water by adding apple juice to the green plum mixed solution.

In the method of manufacturing Pseudomonas mushroom medium according to an embodiment of the present invention, since the enzymes acting in a water-soluble environment using liquid seeds are fast, and nutrients are dissolved and used in water, the hyphae of Pseudomonas mushrooms are more than in water-insoluble seeds. It has the effect of making it grow faster.

In addition, in the method of manufacturing Pseudomonas mushroom medium according to an embodiment of the present invention, by drying brown rice immersed in Psoriasis mushroom extract and using them for manufacturing Pseudomonas mushrooms, as well as compensating for the weaknesses of Pseudomonas mushrooms and digesting them. It has the effect of making it better.

In addition, in the method of manufacturing Pseudomonas mushroom medium according to an embodiment of the present invention, apple juice is added to the citron extract and can be extracted, so that the effect of apples can be obtained, as well as supplementing the weaknesses of Pseudomonas mushrooms and green plums, It has an excellent effect.

1 is a flow chart showing the flow of a method for producing a mushroom culture medium.
2 is a flow chart showing the flow of a method for producing a green plum extract.

Hereinafter, the description of the present invention with reference to the accompanying drawings is not limited to a specific embodiment, and various transformations may be applied and various embodiments may be provided. In addition, the content described below should be understood to include all conversions, equivalents, and substitutes included in the spirit and scope of the present invention.

In the following description, terms such as first and second are terms used to describe various elements, and their meanings are not limited thereto, and are used only for the purpose of distinguishing one element from other elements.

The same reference numbers used throughout this specification denote the same elements.

The singular expression used in the present invention includes a plurality of expressions unless the context clearly indicates otherwise. In addition, terms such as "comprise", "include" or "have" described below are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification. It is to be construed and not to preclude the possibility of the presence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

Hereinafter, a method of manufacturing a mushroom medium according to an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 2.

1 is a flow chart showing a method of manufacturing a mushroom culture medium according to an embodiment of the present invention.

Referring to FIG. 1, a method for manufacturing a mushroom medium according to an embodiment of the present invention includes a step of manufacturing a grain medium using brown rice and water (S100); The step of preparing a liquid mushroom spawn (S200); Inoculating the liquid seed into the prepared grain medium (S300) and culturing the inoculated grain medium (S400); And it may include the step (S500) of cultivating the mushroom.

Specifically, in the grain medium manufacturing step (S100) using brown rice and water, 75 to 90 parts by weight of brown rice and 10 to 15 parts by weight of water may be mixed.

The mixture of brown rice and water is sterilized for 1 to 2 hours at a high temperature of 120°C or higher, and then cooled to 24 to 27°C. The cooling of the grain medium is cooled in a clean room that creates a sterile environment to prepare a sterile grain medium. can do.

At this time, the cooling of the grain medium can be cooled in a cooling chamber capable of creating a sterile environment, and dehumidification can be performed using a desiccant such as silica gel, calcium chloride or concentrated sulfuric acid to prevent the generation of various germs due to moisture. .

The step (S200) of preparing liquid seed mushrooms includes 45 to 60 parts by weight of brown rice, 5 to 10 parts by weight of fructose, 5 to 10 parts by weight of malt extract, 10 to 15 parts by weight of soybean meal, and 20 to 30 parts by weight of distilled water. By mixing 25 to 35 parts by weight of the part or green plum extract, it may be autoclaved at 115 to 135°C for 60 to 90 minutes, and cultured at 25 to 27°C for 8 to 12 days to prepare a culture solution.

At this time, if the sterilization time of the liquid spawn is less than 60 minutes, heat is not evenly transferred to the inside of the liquid spawn, so it is difficult to expect a sterilization effect, and if it exceeds 90 minutes, excessive treatment costs may occur.

In addition, the sterilized liquid seed can be cooled in a cooling room at 8 to 13°C, and the cooled liquid seed may be allowed to stand or shake for 8 to 12 days to prepare a culture solution.

At this time, referring to FIG. 2 for the mixed green plum extract, the green plum extract is a step (S220) of preparing a green plum mixture by mixing the juice and water using the green plum juice obtained from the step (S210) of juicing the green plum. Through it, it is possible to prepare a mixture of green plums. Thereafter, the green plum fruit and apple juice are added to the green plum mixed solution, and hot water extraction is performed (S230) to prepare a green plum extract.

Green plum contains a lot of organic acids such as citric acid, malic acid, and hwabacic acid, so it is good for fatigue recovery, and because it contains pyruvate, it can protect liver and improve liver function. In addition, catechinic acid in plums has detoxification and sterilization effects, so it can inhibit the reproduction of harmful bacteria in the intestine, and when combined with citric acid due to its high calcium content, it can also increase the absorption rate of calcium in the body.

The green plum extract, which is made on the basis of such a green plum, is extracted with hot water by mixing the directly juiced green plum juice with the green plum fruit, so that the maximum effect due to the ingredients of the green plum can be obtained. By allowing the ingredients to be contained, it can have the effect that consumers with gastrointestinal disorders can safely consume it.

Here, in the case of mixing less than 25 parts by weight of the green plum extract in the step (S200) of preparing the Pseudomonas mushroom liquid seed, it is difficult to see the efficacy of the green plums because the ingredients of Psoriasis mushrooms are not sufficiently absorbed into Pseudomonas mushrooms, and the mixture exceeds 35 parts by weight. In case of doing so, there is no difference in its effect, but nutrients are excessively supplied, which may lead to the possibility of growth of other bacteria.

On the other hand, the green plum extract may be hot water extraction by adding apple juice to the green plum mixture obtained by mixing the green plum juice and water.

An apple is a fruit with a warm ingredient and contains pectin, which activates intestinal function, prevents constipation and diarrhea, discharges toxins from the body, and has quercetin, an antioxidant, which helps brain health. Along with it, it complements the cold nature of Pseudomonas mushrooms, and can aid in influence and function.

Such apples are extracted with hot water and green plum extract, so that when brown rice is immersed, the apples are absorbed into the grain medium and are cultivated. have.

On the other hand, the step (S300) of inoculating the liquid seed into the prepared grain medium is inoculated with the protozoa of Pseudomonas mushroom to the culture solution prepared in the step (S200) of preparing the liquid seed of Pseudomonas mushrooms at 25 to 27°C. By injecting air daily, it is possible to prepare liquid spores of Pseudomonas mushrooms.

In addition, the cultivation of the liquid seed inoculated with the protozoa may be performed using a shaking culture method of culturing using a reciprocating or rotary incubator.

Preferably, it is appropriate to inject air for 10 days to prepare a liquid seed, and in this case, the injection of air is a swing culture method in which oxygen is supplied by increasing the contact area between the culture solution and the air layer during shaking, or the compressed air is forced into the culture solution. It can be supplied using the aeration culture method in which it is blown.

In general, liquid spawn refers to a spawn grown in a liquid medium that dissolves in water, such as glucose, sugar, wort, and yeast juice, unlike sawdust spawn, which is cultivated in a solid medium using sawdust and rice bran as a nutrient source from the hyphae of edible mushrooms. can do.

Sawdust spawn is not only a component in which the medium component is insoluble in water as sawdust and rice bran, but also the properties and state of the spawn are solid. However, sawdust spawns have a moisture content of 60 to 70%, whereas liquid spawns contain almost 100% of the media components, so the moisture content of the media is close to 100%, so the nature and state of the spawn must be liquid. .

In order to absorb nutrients necessary for the hyphae of edible mushrooms, enzymes necessary for nutrient decomposition are secreted from the outside of the mycelium sebo, decomposed into a structure that is easy to absorb nutrients inside the hyphae cells, and then absorbed into the hyphae cells. Since these enzymes always take place in an aqueous solution environment, all nutrients are used for the growth of mycelium, so edible mushrooms in a liquid medium containing nutrients in water-soluble properties rather than in sawdust spawns that use insoluble sawdust and rice bran as a nutrient source. There is a characteristic that the growth of the mycelium is much faster.

Meanwhile, in the step of culturing the inoculated grain medium (S400), the cultured grain medium by inoculating the liquid seed may be cultured in a clean room at 24 to 27°C for 60 to 90 days.

In addition, in the step of cultivating Pseudomonas mushrooms (S500), the cultured medium in the step of culturing the inoculated grains (S400) may be inoculated into oaks to cultivate Pseudomonas mushrooms.

Hereinafter, the following Examples and Experimental Examples are presented in order to more specifically explain the method of manufacturing Pseudomonas mushroom medium according to an embodiment of the present invention, but the following Examples and Experimental Examples are only illustrative of the present invention, and the rights of the present invention are It is not limited to the following Examples and Experimental Examples.

[Example]

80 parts by weight of brown rice, 5 parts by weight of water, and 5 parts by weight of a nutrient mixture were mixed and sealed, followed by sterilization at 120° C. for 90 minutes. Then, it was cooled to 25° C. in a sterile state to prepare a grain medium, and the prepared medium was cut into 4 horizontal rows and 3 vertical rows to form 12 incisions. At this time, the nutrient mixture is a mixture of sugar, corn flour, buckwheat, coffee grounds, herbs, germanium, green tea, and calcium carbonate.

The liquid seeds of Pseudomonas mushrooms are mixed with 70 parts by weight of brown rice, 5 parts by weight of fructose, 5 parts by weight of malt extract, 5 parts by weight of soybean meal, and 10 parts by weight of green plum extract, followed by high-pressure sterilization at 120°C for 90 minutes, and then at 25°C for 10 days. A culture solution was prepared by culturing. Then, after inoculating the Pseudomonas Protozoa into the prepared culture solution, air was injected by shaking culture at 25° C. for 10 days to prepare a liquid seed. After that, the liquid seed was inoculated into a grain medium, cultured at 25°C, and the mushrooms were cultivated after 60 days.

[Comparative Example 1]

Except for the fact that the liquid spawn of the above example was used as a rice cultivar spawn, the mushrooms were cultivated in the same manner as in the example. The rice-grown spawn is immersed in 75 parts by weight of 25 parts by weight of the rice-grown cut into 6 cm for 24 hours, and then 2 parts by weight of calcium carbonate and a nutrient mixture are added and mixed. After that, it is placed in a seed bottle and a medium is added, and then the entrance is tightly closed with a cotton pad. After that, it was sterilized at 120° C. for 90 minutes to prepare and use rice-grown spores.

[Comparative Example 2]

Except for the fact that the liquid spawn of the above example was used as the sawdust spawn, the mushrooms were cultivated in the same manner as in the example. The sawdust spawn is mixed with 80 parts by weight of sawdust, 15 parts by weight of rice bran and 45 parts by weight of water. After that, it is placed in a seed bottle and a medium is added, and then the entrance is tightly closed with a cotton pad. After that, it was sterilized at 120° C. for 90 minutes to prepare and use sawdust spawns.

[Comparative Example 3]

Except that the liquid seed of the above example did not use the extract of green plum, the mushrooms were cultivated in the same manner as in the example.

[Comparative Example 4]

The same as in Example, except that 50 parts by weight of brown rice, 20 parts by weight of potato powder, 5 parts by weight of fructose, 5 parts by weight of malt extract, 5 parts by weight of soybean meal, and 10 parts by weight of green plum extract were mixed with the liquid seed of the above example. Psoriasis mushrooms were grown.

[Comparative Example 5]

Except for the fact that the nutrient mixture of the above example was composed of only sugar, the mushrooms were cultivated in the same manner as in the example.

Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Growth rate (%/10 days) 97 65 67 90 87 79 Production (g) 480 403 411 461 455 418 Size (diameter cm) 9 6 6.1 8.8 8 7.1 Badge success rate (%) 94 70 77 89 85 87

As can be seen from Table 1, it can be seen that, compared to Comparative Examples 1 to 5, the growth rate was significantly higher in the examples of the present invention, and the production amount and size were also significantly higher.

In addition, it can be seen that the medium success rate is also higher than that of Comparative Examples 1 to 5.

Therefore, it was determined that when cultivation of Pseudomonas mushrooms according to the present invention, stable, efficient, and high-quality cultivation of Pseudomonas mushrooms is possible.

Although the embodiments of the present invention have been described above with reference to the accompanying drawings, it will be understood that the present invention can be implemented in other specific forms by those of ordinary skill in the art. Therefore, the embodiments described above are illustrative and non-limiting in all respects.

Claims (8)

In the method for producing a mushroom medium,
A grain medium manufacturing step using grains and water selected from grains such as brown rice, roasted brown rice, and barley;
Preparing a liquid mushroom spawn;
Inoculating the prepared Pseudomonas mushroom liquid spawn into the prepared grain medium;
Culturing the inoculated grain medium; And
A method for manufacturing Pseudomonas mushroom medium comprising the step of cultivating Pseudomonas mushrooms by inoculating the cultured medium to oak trees
The method of claim 1,
The grain medium manufacturing step,
Brown rice and water are sterilized for 1 to 2 hours at a high temperature of 120°C or higher, and then cooled to 24 to 27°C to prepare a sterile grain medium.
The method of claim 1,
The step of preparing the liquid spawn,
A culture mixture of brown rice, fructose, malt extract, soybean meal, distilled water or green plum extract was autoclaved at 115 to 135°C for 60 to 90 minutes, and then cultured at 25 to 27°C for 8 to 12 days to prepare a culture solution. A method for producing Psoriasis mushroom medium, characterized in that
The method of claim 3,
After inoculating Pseudomonas mushroom progenitors in the culture medium, the method further comprises the step of injecting air at 25 to 27°C for 8 to 12 days to prepare a liquid spawn
The method of claim 1,
The step of inoculating the liquid mushroom spawn,
Inoculating the prepared Pseudomonas mushroom liquid seed into the prepared grain medium, and culturing Pseudomonas mushroom medium for 60 to 90 days in a clean room maintained at 24 to 27°C.
The method of claim 1,
The step of cultivating the situation mushrooms,
Pseudomonas mushroom medium manufacturing method, characterized in that cultivation of Pseudomonas mushrooms by inoculating a cultured medium of Pseudomonas mushrooms on oak
The method of claim 3,
The green plum extract,
The green plum extract is an extract obtained by hot water extraction by adding green plum fruit to a green plum mixture obtained by juicing green plum juice and water.
The method of claim 7,
The green plum extract,
A method for producing Pseudomonas mushroom medium, characterized in that hot water is extracted by adding apple juice to the green plum mixture

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