KR20190115150A - The Kimchi using Fermented Black-ginseng with increased Rg3 Contents using Paenibacillus cellulositrophicus and the Method for preparing the same - Google Patents
The Kimchi using Fermented Black-ginseng with increased Rg3 Contents using Paenibacillus cellulositrophicus and the Method for preparing the same Download PDFInfo
- Publication number
- KR20190115150A KR20190115150A KR1020180037350A KR20180037350A KR20190115150A KR 20190115150 A KR20190115150 A KR 20190115150A KR 1020180037350 A KR1020180037350 A KR 1020180037350A KR 20180037350 A KR20180037350 A KR 20180037350A KR 20190115150 A KR20190115150 A KR 20190115150A
- Authority
- KR
- South Korea
- Prior art keywords
- ginseng
- extract
- fermented black
- kimchi
- fermented
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 235000021109 kimchi Nutrition 0.000 title claims abstract description 18
- 241000837711 Paenibacillus cellulositrophicus Species 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title claims description 7
- 241000208340 Araliaceae Species 0.000 claims abstract description 58
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 58
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 58
- 235000008434 ginseng Nutrition 0.000 claims abstract description 58
- 244000305267 Quercus macrolepis Species 0.000 claims abstract description 25
- 244000005700 microbiome Species 0.000 claims abstract description 16
- 239000000284 extract Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 235000021574 pickled cabbage Nutrition 0.000 claims description 13
- 235000020710 ginseng extract Nutrition 0.000 claims description 11
- 240000007124 Brassica oleracea Species 0.000 claims description 9
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 9
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 9
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 238000011049 filling Methods 0.000 claims description 7
- 235000011194 food seasoning agent Nutrition 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 240000004160 Capsicum annuum Species 0.000 claims description 5
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 claims description 5
- 235000007862 Capsicum baccatum Nutrition 0.000 claims description 5
- 244000088415 Raphanus sativus Species 0.000 claims description 5
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 5
- 239000001728 capsicum frutescens Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 241000251468 Actinopterygii Species 0.000 claims description 4
- 229920002678 cellulose Polymers 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000011033 desalting Methods 0.000 claims description 3
- 238000009966 trimming Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 2
- RWXIFXNRCLMQCD-JBVRGBGGSA-N (20S)-ginsenoside Rg3 Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1CC[C@]2(C)[C@H]3C[C@@H](O)[C@H]4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@@H]4[C@@](C)(O)CCC=C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RWXIFXNRCLMQCD-JBVRGBGGSA-N 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 229930182494 ginsenoside Natural products 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 4
- 238000010025 steaming Methods 0.000 description 4
- 235000002789 Panax ginseng Nutrition 0.000 description 3
- 230000003712 anti-aging effect Effects 0.000 description 3
- 230000001093 anti-cancer Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 240000002234 Allium sativum Species 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000004611 garlic Nutrition 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 241000234282 Allium Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- XIRZPICFRDZXPF-UHFFFAOYSA-N Ginsenoside Rg3 Natural products CC(C)=CCCC(C)(O)C1CCC(C2(CC(O)C3C4(C)C)C)(C)C1C(O)CC2C3(C)CCC4OC1OC(CO)C(O)C(O)C1OC1OC(CO)C(O)C(O)C1O XIRZPICFRDZXPF-UHFFFAOYSA-N 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000007215 black sesame Nutrition 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 229940089161 ginsenoside Drugs 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 210000003899 penis Anatomy 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
- A23B7/10—Preserving with acids; Acid fermentation
- A23B7/105—Leaf vegetables, e.g. sauerkraut
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/20—Products from fruits or vegetables; Preparation or treatment thereof by pickling, e.g. sauerkraut or pickles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/2124—Ginseng
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
본 기술은 참나무에서 추출한 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물로 구성된 추출액에 수삼을 침진 시킨 후 15일 동안 수삼을 발효시켜 Rg3 함유량을 증가시킨 흑삼을 이용한 김치 및 이를 제조하는 방법에 관한 것이다.This technology relates to kimchi using black ginseng with increased Rg3 content by fermenting ginseng for 15 days after impregnating fresh ginseng into extracts composed of Paenibacillus cellulositrophicus microorganisms extracted from oak trees will be.
Description
본 기술은 참나무에서 추출한 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물로 구성된 추출액에 수삼을 침진 시킨 후 15일 동안 수삼을 발효시켜 Rg3 함유량을 증가시킨 흑삼을 이용한 김치 및 이를 제조하는 방법에 관한 것이다.This technology relates to kimchi using black ginseng with increased Rg3 content by fermenting ginseng for 15 days after impregnating fresh ginseng into extracts composed of Paenibacillus cellulositrophicus microorganisms extracted from oak trees will be.
일반적으로, 김치는 배추나 무 등과 같은 주재료에 보조 재료로서 고춧가루, 마늘, 파, 생강, 젓갈 등과 같은 양념을 혼합하여 발효시킨 식품이다. 김치는 무기물과 비타민(vitamin)이 다량 함유되어 있을 뿐만 아니라 인체에 유익한 유산균이 매우 풍부하여 우리의 식생활에서 없어서는 안될 가장 중요한 음식이라 할 수 있으며, 인체의 강장작용에도 탁월한 효과를 발휘하는 건강식품이라 할 수 있다. In general, kimchi is a food fermented by mixing seasonings such as red pepper powder, garlic, green onion, ginger, salted fish, and the like as main ingredients such as cabbage or radish. Kimchi is not only rich in minerals and vitamins, but also rich in lactic acid bacteria that are beneficial to the human body, making it one of the most important foods that are indispensable in our diet. can do.
최근 들어, 김치가 항암작용, 항노화작용 및 면역증강 등에 탁월한 효과가 있음이 알려지면서 세계인의 건강식품으로 거듭나고 있다. 보다 우수한 효과를 나타내기 위하여 김치의 효능을 증진시키기 위하여 다양한 소재를 접목시키기 위한 연구가 활발히 진행되고 있다.Recently, it is known that kimchi has an excellent effect on anticancer, anti-aging and immune enhancement, etc., and has been reborn as a health food of the world. In order to improve the efficacy of kimchi in order to show a more excellent effect research to combine a variety of materials are actively in progress.
한편, 흑삼은 인삼을 증숙과 건조 과정을 여러 차례 되풀이하여 얻어지는 것으로서 통상 구증구포(아홉 번 찌고 아홉 번 말림) 공정으로 제조된다. 이와 같이 찌고 건조시키는 공정을 거치면서 인삼에 함유되지 않은 진세노사이드가 새로 생성되기도 하며, 원래 진세노사이드의 함량에도 많은 변화가 생기게 된다. 그 결과, 인삼과는 다른 우수한 약리효과를 나타내게 되며, 보존성도 좋아진다. 그리하여 수삼이나 백삼 대신 흑삼, 흑홍삼 등을 이용한 건강기능성 식품, 약학 조성물 등의 개발이 활발히 이루어지고 있다.On the other hand, black ginseng is obtained by repeating the process of steaming and drying ginseng several times and is usually manufactured by a process of gujeunggu (steaming nine times and drying nine times). As a result of the steaming and drying process, ginsenosides, which are not contained in ginseng, are newly generated, and a lot of changes occur in the content of the original ginsenosides. As a result, it exhibits excellent pharmacological effects different from ginseng, and improves preservation. Therefore, development of health functional foods, pharmaceutical compositions, etc. using black ginseng and black red ginseng instead of ginseng or white ginseng is being actively made.
진세노사이드는 인체 대사과정 중 고분자 진세노사이드가 저분자 진세노사이드나 사포닌 대사물로 전환되어 흡수되는데, 흑삼은 인삼, 홍삼에 함유된 고분자 진세노사이드(Rg1, Rb1 등)를 흡수하기 쉬운 형태의 Rg3 등의 저분자 진세노사이드나 사포닌 대사물로 바꿔 흡수율을 높일 수 있어, 인삼 또는 홍삼을 장기간 복용해야 효과를 볼 수 있는 단점을 보완 할 수 있다.Ginsenoside is absorbed by converting high-molecular ginsenosides into low-molecular ginsenosides or saponin metabolites during human metabolism. Black ginseng is easily absorbed by ginsenosides (Rg1, Rb1, etc.) contained in ginseng and red ginseng. It is possible to increase the absorption rate by switching to low molecular weight ginsenosides or saponin metabolites such as Rg3, to compensate for the disadvantages to take long-term effects of ginseng or red ginseng.
최근, 이와 같이 저분자 진세노이드의 함량을 높이기 위해 미생물 발효 공법을 이용한 흑삼 제조에 대한 연구가 진행되고 있으며, 기존의 증숙과 건조를 수차례 반복하는 과정이 아닌 경제성이 좋으면서 저분자 진세노이드의 함량이 높은 흑삼을 제조하기 위한 방법 연구가 활발히 진행되고 있다.Recently, research on the preparation of black ginseng using a microbial fermentation method has been conducted to increase the content of the low molecular weight ginsenoids. Research into a method for producing high black ginseng is actively progressing.
본 발명의 발효 흑삼의 제조 방법에 있어서 예의 연구 검토한 결과,As a result of earnest research and investigation in the manufacturing method of fermented black ginseng of the present invention
참나무에서 추출한 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물을 이용하여 인삼의 유효성분인 저분자 진세노사이드 Rg3의 함량을 극대화 시킨 발효 흑삼을 이용한 김치 및 이를 제조하는 방법을 발견하고 본 발명을 완성하였다.Finding Kimchi using fermented black ginseng maximized the content of low molecular ginsenoside Rg3, the active ingredient of ginseng, using the Panibacillus cellulositrophicus microorganism extracted from oak, and a method for producing the same It was.
한편으로, 본 발명은On the other hand, the present invention
참나무에서 추출한 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물 함유 추출액을 이용하여 Rg3 함유량을 증가시킨 발효 흑삼을 이용한 김치를 제공한다.It provides kimchi using fermented black ginseng with increased Rg3 content by using an extract containing Paenibacillus cellulositrophicus microorganism extracted from oak.
다른 한편으로, 본 발명은On the other hand, the present invention
(i) 배추를 손질하여 절인 후 탈염하고 탈수시켜 절임배추를 준비하는 단계;(i) preparing the pickled cabbage by trimming and then desalting and dehydrating the cabbage;
(ii) 고춧가루, 무 및 젓갈을 포함하는 양념을 준비하는 단계; (ii) preparing a seasoning comprising red pepper powder, radish and salted fish;
(iii) 상기 절임배추에 준비된 양념을 속넣기하는 단계;(iii) incorporating the seasoning prepared in the pickled cabbage;
(iv) 속넣기가 끝난 절임배추를 보관용기에 채워주고 절임배추가 잠길 정도로 발효 흑삼 추출액을 채워주는 단계; 및(iv) filling the finished pickled cabbage into a storage container and filling the fermented black ginseng extract to the extent that the pickled cabbage is submerged; And
(v) 발효 흑삼 추출액에 잠긴 상태로 5 내지 7일간 숙성시키는 단계;를 포함하는 거을 특징으로 하는 패니바실루스 셀룰로시트로피쿠스를 이용하여 Rg3 함유량을 증가시킨 발효 흑삼을 이용한 김치의 제조방법을 제공한다.(v) aging for 5 to 7 days in a state of immersion in fermented black ginseng extract provides a method of producing kimchi using fermented black ginseng with increased Rg3 content using a panibacillus cellulose citropicus, characterized in that it comprises a do.
본 발명에 따른 발효 흑삼을 이용한 김치는 흡수하기 쉬운 형태의 Rg3 등의 저분자 진세노이드를 높은 함량으로 포함하여 항암작용, 항노화작용 및 면역증강 등의 효능이 향상될 수 있다. Kimchi using fermented black ginseng according to the present invention may include a high content of low-molecular ginsenosides such as Rg3 in an easy-to-absorb form, thereby improving the efficacy of anticancer, anti-aging and immune enhancement.
이하, 본 발명을 보다 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명의 일 실시형태에 따른 김치는Kimchi according to one embodiment of the present invention
참나무에서 추출한 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물 함유 추출액을 이용하여 Rg3 함유량을 증가시킨 발효 흑삼을 이용하는 것을 특징으로 한다.It is characterized by using a fermented black ginseng with increased Rg3 content by using an extract containing Paenibacillus cellulositrophicus microorganism extracted from oak.
본 발명에 따르면, 상기 참나무에서 추출한 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물로 구성된 발효액에 수삼을 침진시킨 후 15일 동안 발효시켜 Rg3 함량이 증대된 발효 흑삼을 이용하여 김치를 제조함으로써, 항암작용, 항노화작용 및 면역증강 등의 김치의 효능이 향상될 수 있다.According to the present invention, by impregnating fresh ginseng into a fermentation broth consisting of Paenibacillus cellulositrophicus microorganism extracted from the oak, and fermentation for 15 days to produce kimchi using fermented black ginseng with increased Rg3 content, The efficacy of kimchi, such as anti-cancer, anti-aging and immune enhancement can be improved.
본 발명의 일 실시형태에 따른 패니바실루스 셀룰로시트로피쿠스를 이용하여 Rg3 함유량을 증가시킨 발효 흑삼을 이용한 김치의 제조방법은Method for producing kimchi using fermented black ginseng with an increased Rg3 content by using Fanibacilli cellulose citrocipicus according to an embodiment of the present invention
(i) 배추를 손질하여 절인 후 탈염하고 탈수시켜 절임배추를 준비하는 단계;(i) preparing the pickled cabbage by trimming and then desalting and dehydrating the cabbage;
(ii) 고춧가루, 무 및 젓갈을 포함하는 양념을 준비하는 단계; (ii) preparing a seasoning comprising red pepper powder, radish and salted fish;
(iii) 상기 절임배추에 준비된 양념을 속넣기하는 단계;(iii) incorporating the seasoning prepared in the pickled cabbage;
(iv) 속넣기가 끝난 절임배추를 보관용기에 채워주고 절임배추가 잠길 정도로 발효 흑삼 추출액을 채워주는 단계; 및(iv) filling the finished pickled cabbage into a storage container and filling the fermented black ginseng extract to the extent that the pickled cabbage is submerged; And
(v) 발효 흑삼 추출액에 잠긴 상태로 5 내지 7일간 숙성시키는 단계;를 포함하는 것을 특징으로 한다.(v) aging for 5 to 7 days while submerged in the fermented black ginseng extract; characterized in that it comprises a.
본 발명의 일 실시형태에 따른 상기 발효 흑삼 추출액의 제조방법은,Production method of the fermented black ginseng extract according to an embodiment of the present invention,
(i) 수삼을 세척하는 단계;(i) washing the fresh ginseng;
(ii) 세척된 수삼을 수분 함유량이 40 내지 60 %가 될 때까지 50 내지 60 ℃의 온도에서 12 내지 24시간 동안 1차 건조하는 단계;(ii) first washing the washed ginseng for 12 to 24 hours at a temperature of 50 to 60 ° C. until the water content is 40 to 60%;
(iii) 1차 건조된 수삼을 참나무 추출 발효액에 24 내지 48 시간 침진시켜 상기 발효액 내에 포함된 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물을 상기 수삼에 침투시키는 단계;(iii) infiltrating the first dried ginseng into oak extract fermentation broth for 24 to 48 hours to infiltrate the ginseng with the Panibacillus cellulositrophicus microorganisms contained in the fermentation broth;
(iv) 상기 미생물이 침투된 수삼을 70 내지 80 ℃의 온도에서 10 내지 15 일 동안 증숙시켜 상기 수삼을 발효시키는 단계; 및(iv) fermenting the ginseng by steaming the ginseng penetrated by the microorganism at a temperature of 70 to 80 ℃ for 10 to 15 days; And
(v) 상기 발효된 수삼을 수분 함유량이 10 % 미만이 될 때까지 60 내지 70 ℃의 온도에서 24 내지 48 시간 동안 2차 건조하는 단계;를 포함하는 것을 특징으로 한다.(v) secondary drying the fermented ginseng for 24 to 48 hours at a temperature of 60 to 70 ℃ until the water content is less than 10%; characterized in that it comprises a.
본 발명의 일 실시형태에 따른 상기 참나무 추출 발효액의 제조방법은,Method for producing the oak extract fermentation broth according to an embodiment of the present invention,
(i) 참나무의 줄기 및 뿌리를 자연 건조하는 단계;(i) naturally drying the trunks and roots of the oak;
(ii) 건조된 참나무의 줄기 및 뿌리와 물을 부피비 1:1로 혼합하고, 열수 추출을 수행하는 단계;(ii) mixing the dried oak stems and roots with water in a volume ratio of 1: 1 and performing hydrothermal extraction;
(iii) 물을 제거하고, 70 내지 90 ℃의 온도에서 5 내지 7 일 동안 발효하는 단계;(iii) removing water and fermenting at a temperature of 70-90 ° C. for 5-7 days;
(iv) 발효된 참나무의 줄기 및 뿌리를 자연 건조하는 단계; 및(iv) naturally drying the stems and roots of the fermented oak; And
(v) 건조된 참나무의 줄기 및 뿌리와 물을 부피비 1:1로 혼합하고, 열수 추출하여 발효액을 제조하는 단계;를 포함하는 것을 특징으로 한다.(v) mixing the dried oak stems and roots with water in a volume ratio of 1: 1, and extracting hot water to prepare a fermentation broth.
상기 참나무에서 추출한 발효액을 한국미생물보존센터에 의뢰하여 동정분석을 한 결과 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물로 구성된 것을 확인하였으며, 현재 한국미생물보존센터에 기탁한 상태이다.The fermentation broth extracted from the oak was commissioned by the Korea Center for Microbiological Conservation and identified as a result of the determination of Panibacillus cellulositrophicus microorganisms and is currently deposited in the Korea Center for Microbiological Conservation.
이하, 실시예에 의해 본 발명을 보다 구체적으로 설명하고자 한다. 이들 실시예는 오직 본 발명을 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 국한되지 않는다는 것은 당업자에게 있어서 자명하다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it is apparent to those skilled in the art that the scope of the present invention is not limited to these examples.
제조예 1 : 참나무 추출 발효액의 제조Preparation Example 1 Preparation of Oak Extract Fermentation Broth
1. 참나무의 줄기와 뿌리를 일정량 채취하였다. 이때, 줄기와 뿌리의 비율은 일정하지 않아도 된다.1. The stems and roots of oaks were harvested. At this time, the ratio of the stem and the root does not have to be constant.
2. 채취한 참나무의 줄기와 뿌리를 대략 50mm 간격으로 잘라 재료를 준비 하였다.2. The trunk and roots of the collected oak were cut about 50mm apart to prepare the material.
3. 준비된 참나무 재료를 72시간 동안 자연 건조시켰다.3. The prepared oak material was naturally dried for 72 hours.
4. 건조된 참나무 재료를 용기에 넣은 후 재료와 부피비가 1:1이 되도록 물을 채운 후 90℃ 정도에서 물이 끊기 시작할 때까지 열수 추출을 하였다. 이때, 90℃ 이상의 온도에서 생존할 수 없는 미생물을 제거하기 위한 살균 작업의 역할도 함께 수행할 수 있다.4. The dried oak material was placed in a container, filled with water so that the material and volume ratio was 1: 1, and then extracted with hot water until the water started to be stopped at about 90 ° C. At this time, it can also perform the role of sterilization work to remove microorganisms that can not survive at a temperature of 90 ℃ or more.
5. 끊인 물은 버리고 참나무 재료만을 취하였다.5. Discard the water and take only oak material.
6. 약 80%의 수분을 가지고 있는 참나무 재료를 일정 용기에 넣은 후 70~ 90℃의 온도에서 5~7일 동안 발효시켰다.6. The oak material, which has about 80% moisture, was placed in a container and fermented at a temperature of 70-90 ° C. for 5-7 days.
7. 발효가 끝난 참나무 재료를 72시간 동안 자연 건조시켰다.7. The fermented oak material was naturally dried for 72 hours.
8. 자연 건조가 끝난 참나무 재료를 일정 용기에 넣은 후 물을 부피 비로 1:1로 맞추어 넣고 24시간 동안 열수 추출하여 발효액을 제조하였다.8. After the natural dried oak material was placed in a container, water was adjusted at a volume ratio of 1: 1, and hot water was extracted for 24 hours to prepare a fermentation broth.
실시예 1 : 참나무 추출 발효액을 이용한 흑삼 추출액의 제조Example 1 Preparation of Black Ginseng Extract Using Oak Extract Fermentation Solution
1. 수삼을 세척하였다.1. Wash ginseng.
2. 세척된 수삼을 발효액에 침진 시키기 전 발효액 내 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물이 수삼에 잘 침투할 수 할 수 있도록 수삼의 수분 함유량이 40~60% 될 때까지 수삼을 1차 건조시켰다. 이때, 건조 조건은 60℃, 12시간 항온 조건으로 수행하였다.2. Before immersing the washed ginseng into the fermentation broth, remove the ginseng until the moisture content of the fresh ginseng is 40 ~ 60% so that the microorganisms of the penis bacillus cellulositrophicus can penetrate the fresh ginseng. Tea was dried. At this time, the drying conditions were carried out at 60 ℃, constant temperature conditions for 12 hours.
3. 1차 건조된 수삼을 참나무 추출 발효액에 24시간 침진시켜 발효액 내 미생물을 수삼에 침투시켰다.3. The first dried ginseng was soaked in oak extract fermentation for 24 hours to infiltrate microorganisms in the fermentation broth.
4. 상기 미생물이 침투된 수삼을 70 내지 80 ℃의 항온 조건으로 15 일 동안 증숙시켜 상기 수삼을 발효하였다.4. The ginseng in which the microorganism penetrated was steamed for 15 days at a constant temperature of 70 to 80 ℃ to ferment the ginseng.
5. 발효된 수삼을 70℃ 48시간(2일) 동안 항온조건으로 수분이 10%가 될 때까지 2차 건조하여 발효 흑삼을 제조하였다.5. Fermented ginseng was dried secondly at 70 ° C. for 48 hours (2 days) under constant temperature until moisture reached 10% to prepare fermented black ginseng.
6. 상기 발효 흑삼을 일정 용기에 넣은 후 물을 부피 비로 1:1로 맞추어 넣고 24시간 동안 열수 추출하여 추출액을 제조하였다.6. The fermented black ginseng was put in a certain container, water was adjusted to 1: 1 in volume ratio, and hot water was extracted for 24 hours to prepare an extract.
실시예 2 : 흑삼 추출액을 이용한 김치의 제조Example 2 Preparation of Kimchi Using Black Ginseng Extract
1. 배추는 개끗이 손질하여 뿌리쪽에서 부터 칼을 넣어 반으로 가른 후 햇볕에 3시간정도 수분을 건조시켰다.1. Clean the cabbage, put a knife from the root side, cut it in half, and then dry the moisture for 3 hours in the sun.
2. 항아리나 넓은통에 소금 10%농도의 소금물에 건조된 배추를 담고 위에 소금을 조금 더 뿌려주었다.2. Put dried Chinese cabbage in 10% salt of brine in a jar or barrel and sprinkle some more salt on top.
3. 숨이 잘 죽은 배추는 조직이 상하지 않게 조심해서 찬물에 두세번 행군 후 소쿠리에서 물기를 빼주었다.3. The cabbage, which died of breath, was carefully removed from the colander after marching two or three times in cold water, so as not to damage the tissue.
4. 배추 40kg일때 고추분, 청량/홍초, 마늘, 소금, 물엿, 새우젓갈, 생새우, 잣, 검은깨, 무, 생삼편 및 다시마채를 26.7 : 6.7 : 5.6 : 3.3 : 11.1 : 11.1 : 11.1 : 1.1 : 1.1 : 6.7 : 8.9 : 6.7(중량%)의 비율로 섞어 총중량 9000g의 양념을 만든 후 넓은 그릇에 섞어 주었다.4. When cabbage 40kg, red pepper powder, soft / red vinegar, garlic, salt, starch syrup, salted shrimp, fresh shrimp, pine nut, black sesame, radish, fresh ginseng, and kelp chop 26.7: 6.7: 5.6: 3.3: 11.1: 11.1: 11.1 : 1.1: 1.1: 6.7: 8.9: 6.7 (% by weight) of the mixture to make a total weight of 9000g of spices and mixed in a wide bowl.
5. 물기를 제거한 배추에 양념을 버무리고 속넣기해주었다.5. Mix the seasoned cabbage and mix it in.
6. 속넣기가 끝난 배추를 보관용기에 채워주고 절임배추가 잠길 정도로 발효 흑삼 추출액을 채워준 다음, 실온에서 15 시간 정도 방치해 두었다가 0 내지 8 ℃에서 냉장보관 하였다. 6. After filling the inner cabbage into the storage container and the fermented black ginseng extract to the extent that the pickled cabbage was submerged, it was left at room temperature for 15 hours and then refrigerated at 0 to 8 ℃.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 본 발명이 속한 기술분야에서 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아님은 명백하다. 본 발명이 속한 기술분야에서 통상의 지식을 가진 자라면 상기 내용을 바탕으로 본 발명의 범주 내에서 다양한 응용 및 변형을 행하는 것이 가능할 것이다.As described above in detail a specific part of the present invention, for those skilled in the art to which the specific description is only preferred embodiments, it is obvious that the scope of the present invention is not limited thereto. Do. Those skilled in the art to which the present invention pertains will be able to make various applications and modifications within the scope of the present invention based on the above contents.
따라서, 본 발명의 실질적인 범위는 첨부된 특허청구범위와 그의 등가물에 의하여 정의된다고 할 것이다.Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.
Claims (3)
(ii) 고춧가루, 무 및 젓갈을 포함하는 양념을 준비하는 단계;
(iii) 상기 절임배추에 준비된 양념을 속넣기하는 단계;
(iv) 속넣기가 끝난 절임배추를 보관용기에 채워주고 절임배추가 잠길 정도로 발효 흑삼 추출액을 채워주는 단계; 및
(v) 발효 흑삼 추출액에 잠긴 상태로 5 내지 7일간 숙성시키는 단계;를 포함하는 거을 특징으로 하는 패니바실루스 셀룰로시트로피쿠스를 이용하여 Rg3 함유량을 증가시킨 발효 흑삼을 이용한 김치의 제조방법.(i) preparing the pickled cabbage by trimming and then desalting and dehydrating the cabbage;
(ii) preparing a seasoning comprising red pepper powder, radish and salted fish;
(iii) incorporating the seasoning prepared in the pickled cabbage;
(iv) filling the finished pickled cabbage into a storage container and filling the fermented black ginseng extract to the extent that the pickled cabbage is submerged; And
(v) fermenting black ginseng extract in a state of immersion for 5 to 7 days; the method of producing kimchi using fermented black ginseng with increased Rg3 content using a panibacillus cellulose citropicus, characterized in that it comprises a.
(i) 수삼을 세척하는 단계;
(ii) 세척된 수삼을 수분 함유량이 40 내지 60 %가 될 때까지 50 내지 60 ℃의 온도에서 12 내지 24시간 동안 1차 건조하는 단계;
(iii) 1차 건조된 수삼을 참나무 추출 발효액에 24 내지 48 시간 침진시켜 상기 발효액 내에 포함된 패니바실루스 셀룰로시트로피쿠스(Paenibacillus cellulositrophicus) 미생물을 상기 수삼에 침투시키는 단계;
(iv) 상기 미생물이 침투된 수삼을 70 내지 80 ℃의 온도에서 10 내지 15 일 동안 증숙시켜 상기 수삼을 발효시키는 단계;
(v) 상기 발효된 수삼을 수분 함유량이 10 % 미만이 될 때까지 60 내지 70 ℃의 온도에서 24 내지 48 시간 동안 2차 건조하여 발효 흑삼을 제조하는 단계; 및
(vi) 상기 발효 흑삼을 용기에 넣은 후 물을 부피 비로 1:1로 맞추어 넣고 24시간 동안 열수 추출하여 추출액을 제조하는 단계;를 포함하여 제조되는 것을 특징으로 하는 패니바실루스 셀룰로시트로피쿠스를 이용하여 Rg3 함유량을 증가시킨 발효 흑삼을 이용한 김치의 제조방법.The method of claim 2, wherein the fermented black ginseng extract is
(i) washing the ginseng;
(ii) first washing the washed ginseng for 12 to 24 hours at a temperature of 50 to 60 ° C. until the water content is 40 to 60%;
(iii) infiltrating the first dried ginseng into oak extract fermentation broth for 24 to 48 hours to infiltrate the ginseng with Panibacillus cellulositrophicus microorganisms contained in the fermentation broth;
(iv) fermenting the ginseng in which the microorganism has penetrated is steamed at a temperature of 70 to 80 ° C. for 10 to 15 days;
(v) preparing the fermented black ginseng by secondary drying the fermented ginseng for 24 to 48 hours at a temperature of 60 to 70 ° C. until the water content is less than 10%; And
(vi) putting the fermented black ginseng into a container and adjusting the water to 1: 1 in a volume ratio to extract hot water for 24 hours to prepare an extract; panicacillus cellulose citropicus Kimchi production method using fermented black ginseng with increased Rg3 content.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020180037350A KR20190115150A (en) | 2018-03-30 | 2018-03-30 | The Kimchi using Fermented Black-ginseng with increased Rg3 Contents using Paenibacillus cellulositrophicus and the Method for preparing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020180037350A KR20190115150A (en) | 2018-03-30 | 2018-03-30 | The Kimchi using Fermented Black-ginseng with increased Rg3 Contents using Paenibacillus cellulositrophicus and the Method for preparing the same |
Publications (1)
Publication Number | Publication Date |
---|---|
KR20190115150A true KR20190115150A (en) | 2019-10-11 |
Family
ID=68210512
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020180037350A Withdrawn KR20190115150A (en) | 2018-03-30 | 2018-03-30 | The Kimchi using Fermented Black-ginseng with increased Rg3 Contents using Paenibacillus cellulositrophicus and the Method for preparing the same |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR20190115150A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100540917B1 (en) | 2003-05-06 | 2006-01-10 | 박순화 | Manufacturing Method of Herbal Kimchi Containing Ginseng |
KR101670810B1 (en) | 2015-11-27 | 2016-10-31 | 재단법인 금산국제인삼약초연구소 | A method for manufacturing a fermented black ginseng containing the increased amount of minor ginsenosides, and the fermented black ginseng thereof |
-
2018
- 2018-03-30 KR KR1020180037350A patent/KR20190115150A/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100540917B1 (en) | 2003-05-06 | 2006-01-10 | 박순화 | Manufacturing Method of Herbal Kimchi Containing Ginseng |
KR101670810B1 (en) | 2015-11-27 | 2016-10-31 | 재단법인 금산국제인삼약초연구소 | A method for manufacturing a fermented black ginseng containing the increased amount of minor ginsenosides, and the fermented black ginseng thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104286159B (en) | A kind of juicy peach fresh-keeping method | |
CN108669501B (en) | Low-nitrite high-dietary-fiber fermented pickle and preparation method thereof | |
CN104286158B (en) | A kind of honey peach gather before preservative specially | |
KR101183541B1 (en) | Making manufacture of black ginseng | |
KR102128913B1 (en) | Method for manufacturing galbi tang and galbi tang manufactured by the method | |
CN102805169B (en) | Mixed effervescence tea drink | |
KR101537581B1 (en) | Acanthopanax leaching fermented tea with a method of manufacturing | |
CN109456868A (en) | A kind of lichee fruit wine processing method | |
KR101199875B1 (en) | Primula fast-fermented bean paste and method thereof | |
KR100961494B1 (en) | Watery radish kimchi using sap and manufacturing method thereof | |
CN106722540A (en) | The rehydration and brewing method of a kind of cowpea semi-dry products | |
KR20140076675A (en) | Manufacturing method of Kimchi with Taraxacum platycarpum Dahlst extract | |
KR20190115150A (en) | The Kimchi using Fermented Black-ginseng with increased Rg3 Contents using Paenibacillus cellulositrophicus and the Method for preparing the same | |
CN109170717A (en) | A kind of Quick production method of pickles | |
CN104012899A (en) | Method for preparing spicy frosted sweet potato leaf shreds by adopting vacuum drying and positive-negative high-pressure low-temperature fermentation technologies | |
KR100607670B1 (en) | Functional Kimchi Prepared Using Ogalpi and Its Manufacturing Method | |
KR101718389B1 (en) | Manufacturing method of kimchi | |
KR101885650B1 (en) | Brewed type citron leaf tea, and manufacturing method thereof | |
CN108967970A (en) | The preparation method of vacuum fermentation sauerkraut | |
CN108703324A (en) | A kind of processing method of course dried pork slice | |
CN109430506A (en) | A kind of preparation method of no seed wrinkled papaya preserved fruit | |
KR102218728B1 (en) | manufacturing method of white Kimchi using cockscomb | |
CN107432447A (en) | A kind of bamboo shoots flavor Huang tribute chopped chilli and preparation method thereof | |
CN107232556A (en) | It is a kind of to chop the preparation method that yellow tribute green pepper pickles crisp cucumber rind | |
CN107279856A (en) | Yellow tribute chopped chilli of a kind of radish local flavor and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20180330 |
|
PG1501 | Laying open of application | ||
PC1203 | Withdrawal of no request for examination |