KR20180108132A - Cosmetic composition for skin wrinkle improvement and prevention - Google Patents

Abstract

The present invention relates to a cosmetic composition for ameliorating or preventing skin wrinkles by solubilizing two or more kinds of hyaluronic acid and rh-oligopeptide. In particular, the present invention relates to a cosmetic composition for skin, to be locally used to increase volume of tissues or lips to the cheekbones or fill and relive fine relief, depressed spots, or surface wrinkles on the body or the face.

Description

[0001] Cosmetic composition for skin wrinkle improvement and prevention [

The present invention relates to a cosmetic composition for alleviating or preventing skin wrinkles in which Al-cholestyptic acid and two or more kinds of hyaluronic acid are encapsulated. The present invention relates to a cosmetic composition for alleviating or preventing wrinkles of skin or wrinkles, depressions or micro- To a cosmetic dermatological composition for topical use for alleviating or increasing the volume of lips or tissues close to the cheekbones.

More particularly, the composition of the present invention relates to a cosmetic composition for improving or preventing skin wrinkles, which is particularly suitable for application to epidermis and dermis of the face.

Wrinkles are wrinkles or furrows of varying depths present on the surface of the skin that are considered signs of skin aging. These involve deficiencies of the skin structure, some areas of the skin, especially facial enlargement or repeated enlargement.

The causes of wrinkle formation are diverse and include skin aging, gravity, decomposition of collagen fibers due to loss of elasticity, external agonist action, daytime sunlight, and repeated muscle and joint movements. The combined action of internal and external factors leads to a faster aging of the skin and the appearance of wrinkles early on.

Internal factors are associated with the physiological processes of tissue aging that progressively contribute to epidermal keratinization, dermal relaxation, muscle relaxation of analogues, and reduced water supply.

Over time, cell turnover of the epidermis becomes increasingly difficult because the stratum corneum becomes drier and thicker, delaying living cells.

At the same time, the deep tissues of the dermis and face (including facial muscle groups and muscles) relax and cause gradual deterioration of tone loss and wrinkles in the tissue. Also, the gradual loss of skin microcirculation makes it difficult to perfect circulation in the outermost layer of the skin.

Therefore, to slow down the aging of the skin can rely on moisturizing and beauty treatment to increase elasticity and elasticity.

Many types of moisturizing cosmetic preparations suitable for treating and alleviating wrinkles are currently commercially available.

In cosmetic medicine, it is known to fill wrinkles by directly injecting a dermis filler into a depression area to increase the volume of a specific area of the face, in order to obtain fillings of the dermis or to place more emphasis.

Among the skin fillers widely used in the practical use of cosmetic preparations, hyaluronic acid is known to play an important role because of its plasticity properties and its ability to fill wrinkles without side effects and maintain calibration for extended periods of time.

Hyaluronic acid is a disaccharide present in living organisms as a component of the extracellular space. This molecule is essential for the formation of collagen matrix and elastic fibers and for maintaining skin moisture.

From a chemical point of view, hyaluronic acid is present in an alternating sequence of linear polymers of N-acetyl-glucosamine and glucuronic acid, and the carboxy group is strongly ionized at a significant pH of the skin surface.

Unlike all other glycosaminoglycans that contain sulfate counterparts, they are small in size (average 80 sugar residues, with a molecular weight of 1,5-2 x 104 Dm) to form a glycoprotein complex (proteoglycan)

In addition, hyaluronic acid is free of sulfur, has a polypeptide component, contains more than 25,000 disaccharides and reaches up to 1000 times (even 2 × 107 Da). On a straight line, the macromolecule can reach a size of 25 μm (three times the diameter of the red blood cell).

The more rigid hyaluronic acid polymer arrangements take an extended arrangement, unlike the protein chains that tend to be arranged in a spherical shape.

The carboxyl groups caused by glucuronic acid confer acidity to the polyglucoside (about 3 pKa): at physiological tissue pH, they are completely dissociated. Hyaluronic acid thus represents a strong anion valence capable of attracting a multitude of cations (mainly Na +). The high expression of the charge contributing to the presence of many hydroxyl groups makes it possible to exert a strong attraction to bipolar water molecules.

The surface of the skin adopts a hard, disordered spiral molecular structure that occupies an increased volume in relation to the size of the polymer and covers it at a relatively low concentration. This characteristic interferes with percutaneous progression and potentially contributes to the maintenance of the surface, Promoting the rapid formation of suitable viscoelastic gels.

However, recent studies have shown that different types of intramolecular hydrogen bonds formed along the sugar chains of hyaluronic acid in the hydrated state.

Hyaluronic acid is present in all human connective tissues, dermis and epidermis. It is produced by fibroblasts and keratinocytes.

It plays an important role in providing elasticity and denseness to the skin because it forms a macromolecular complex that provides a type of support structure by binding to collagen, fibronectin and proteoglycans on the skin. In addition, hyaluronic acid molecules have a high It has capacity to ensure increased hydration and elasticity of the skin.

The horny layer also contains hyaluronic acid, which plays an important role in dehydration. It interacts with lipids in the lamellar structure of the stratum corneum and regulates its mechanical properties.

In addition, hyaluronic acid has a chemical structure such as bacteria, animal or human.

Thus, hyaluronic acid is entirely biocompatible because it is chemically identical in all species and in all types of tissues.

The use of hyaluronic acid has been well documented not only in cosmetic medicine but also in other medical fields such as ophthalmic surgery and orthopedics. Over the past two decades, over 30 million people have been successfully treated with hyaluronic acid-based products.

Based on hyaluronic acid, the medicinal / cosmetic treatment rejuvenates the skin and helps to revitalize the skin and restore lips (fullness and elasticity, volume and contour), wrinkles (eg nasal), facial contours (eg cheeks and chin) , Ear wax, wrinkles), acne scars, breast and body remodeling, and plastic surgery uses a syringe made of thin needles to inject a small amount of hyaluronic acid into the relevant area.

Once administered, it stimulates cellular function and returns the lost compactness to the skin. The filler effect of hyaluronic acid administration depends on the type of skin and the amount and quality of the injection. Hyaluronic acid also promotes the synthesis of new collagen and other extracellular matrix components by stimulating the proliferation of fibroblasts, and is known as an ideal filler because of the reduced risk of allergic reactions due to its absence of antigenicity.

With age, hyaluronic acid is localized, and in aging skin, the amount of hyaluronic acid present in the dermis is markedly reduced, particularly in relation to the reduction of biosynthetic capacity of fibroblasts and their cell proliferative activity.

A major age-related change is the increased need for tissue for hyaluronic acid and the reduction of hyaluronic acid molecules that combine water to hydrate the skin. In this way, it is known that the amount of water retained in the tissue is reduced and induces skin elasticity which is directly connected thereto.

Gradual reduction in the size of hyaluronic acid polymers in the skin is another common feature of aging.

As you get older, the concentration and type of protein molecules that bind to hyaluronic acid polymers also change. These phenomena characterize aging skin and contribute to apparent dehydration, atrophy, and volume reduction.

The process of facial aging is also associated with more complex changes affecting the anatomical structure of the face at all depths. Indeed, bone resorption in the jaw bone and gums causes a gradual decrease in muscle platelet count and a reduced ability to support bone structures associated with subdermal redistribution. All of these diminish the ability of the dermis to thin and support elastic fibers and collagen, gradually modifying the morphological appearance of the face and losing the natural convexity of youth. The complex relationship between hyaluronic acid and skin is documented in the Journal of Investigative Dermatology.

In a 1999 study, T.J. Brown et al. Experimental evidence is provided to support the hypothesis that the very long hydrophilic chains of hyaluronic acid can cross skin barriers. According to these Australian researchers, mucopolysaccharides applied to the skin have spread through the stratum corneum to the epidermis and dermis.

As is known, the deepest part of the stratum corneum has a "cemented wall" structure in which the bricks are filled with keratinocytes, non viable cells, keratin filaments, and tight shell proteins.

T.J. Brown et al. The study can be summarized as follows: Hyaluronic acid on the skin of hairless rats and human volunteers soon disappears from the epidermal surface in areas protected from contact and friction, resulting in rapid absorption. A few hours later, automatic radiography showed that radioactive granules were scattered throughout the thickness of the stratum corneum and scattered in important layers of the epidermis, especially in the basal elements.

Conventionally, for the purpose of improving skin roughness, a method of supplementing the barrier function of each layer by a clogging agent such as a vaseline ointment or a water-in-oil type emulsifying agent, a method of supplementing water content of each layer by a moisturizing agent such as sorbitol, glycerin or an alkylene oxide derivative A method of immersing the skin inflammation by an anti-inflammatory agent such as glycyrrhetinic acid, and a method of activating skin cells by vitamins, hormones, etc. have been used (Patent Documents 1 to 5).

In addition, there are also attempts to apply only hyaluronic acid and derivatives thereof, that is, low-molecular hyaluronic acid to dermis and subcutaneous filler which can be used in cosmetology (Patent Documents 6 to 7).

However, not all of the conventional methods described above can be said to be sufficient for maintaining the skin moisture, and the effect of improving the exfoliation is small. Particularly, when an occlusion agent is used, a drawback that gives unpleasant texture such as stickiness . On the other hand, even in the case of using a moisturizing agent, a large amount of the moisturizing agent must be added in order to enhance the effect. As a result, there has been a problem of giving an unpleasant feeling such as sticky feeling or slippery feeling. Further, when extracts of animal tissues such as placenta, vitamins, hormones and the like are used, there is a problem in terms of safety and stability over time due to side effects and the like. Particularly, with respect to the coarsening of the keratin materials, it has been clarified that the exfoliation of the keratin is not carried out smoothly, but there is no proper countermeasure.

 Meanwhile, human epithelial cell growth factor (hEGF) is composed of 53 amino acids and was first isolated from rat submandibular gland by Dr. Stanley Cohen of the United States. When this substance was treated in a mouse born immediately, the eyelid was faster than normal rat (Chhen, S, J. Biol. Chem., 237, 1555-1562, 1962), which has been experimentally proven to open and is called a factor that promotes epithelial cell growth. Since then, Dr. Stanley Cohen has been awarded the Nobel Prize for Medicine in 1986 for his recognition of EGF. EGF is structurally characterized by a polypeptide having three disulfide bonds (Savage, CTJ Biol. Chem. 248, 7669-7672, 1973, Savage, CR, Jr. et al., J. Biol. Chem. 247, 7612 -7621, 1972), as a factor promoting the growth of epithelial cells, induces the growth and division of mammalian cells, particularly epithelial and skin cells, by stimulating the growth of epithelial cells by transmitting signals through receptors on the cell membrane (Sporn MB, Nature 313, 745-747, 1985, N. Engl., J. Med., 303, 878-880, 1980). It is also known that EFG plays a very important role in the regulation at the molecular level in processes such as wound healing (Buckley, A. et al., Proc Natl Acad Sci USA 82, 7340-7344, 1985)

EGF is present in high concentrations in saliva, urine, milk tears, blood, etc., and is supplied from the blood when it is wounded and acts to heal the wound without scarring. In addition, FSH (Follicle stimulation hormone), which promotes female hormone secretion, matures fertilized eggs in the uterus and helps regenerate the cornea, which is prone to degeneration due to lack of blood vessels. In addition, it promotes cell proliferation of epithelial cells and endothelial cells, promotes cell proliferation of fibroblasts that synthesize collagen as a component of the dermis, induces angiogenesis of skin lesion sites and induces secretion of other regeneration promoting factors. It plays a key role in skin regeneration, including promoting the synthesis of fibronectin, a substance that forms a network

The human body autonomously regulates EGF to restore normal recovery by supplying it properly to the damaged tissue, but if the EGF is not properly supplied, it should be supplied externally to recover normally. In this respect, it can be used in various ways. For example, it can be used for diabetic foot ulceration, corneal injury of the wound, abdominal incision, cosmetic dermabrasion, and aging skin improvement. In addition, EGF can be used in the gastrointestinal (Gregory, H., J. Cell Sci. Suppl. 3, 11-17, 1985), which has been reported to be effective in the treatment of diseases such as gastric ulcers.

Here, in the conventional cosmetic composition for improving or preventing skin wrinkles (dummy cosmetic cosmetic composition), there are many cases where hyaluronic acid and an aldehyde oligopeptide are solely used, and most of them have pursued only the efficacy for each substance. There is no composition for a dermocosmetic cosmetic for improving wrinkles and improving skin dryness and at the same time activating cells of skin tissue.

Patent Document 1: JP-A-6-293625 Patent Document 2: Japanese Patent Application Laid-Open No. 7-277943 Patent Document 3: Japanese Patent Application Laid-Open No. 9-95432 Patent Document 4: Japanese Patent Publication No. 3660656 Patent Document 5: JP-A-2009-227645 Patent Document 6: U.S. Patent Application Publication No. 2011-0118206 Patent Document 7: International Patent Publication No. WO 2014044808 A2

It is an object of the present invention to improve the roughness of skin by hyaluronic acid, in particular, to improve the coarsening and dryness of horny substances, to realize smooth skin by arranging skin texture, and to provide a growth promoting agent, The present invention provides a cosmetic composition for improving or preventing skin wrinkles that stimulates the formation of ECM (extracellular matrix) such as collagen, elastin, fibronectin and the like by stimulating the cells and fibroblasts to activate the cells of the skin tissue.

It is an object of the present invention to provide a cosmetic composition for improving or preventing skin wrinkles which rapidly penetrates the skin on the basis of different types of hyaluronic acid and aloe-oligopeptide-1.

The problems to be solved by the present invention are not limited to those mentioned above, and other solutions not mentioned can be clearly understood by those skilled in the art from the following description.

The inventors of the present invention have conducted intensive studies in order to solve the above problems. As a result, the present inventors have found that, by completing at least one of hyaluronic acid, a hyaluronic acid derivative, and a hyaluronic acid salt and one kind of an aldehyde And have completed the present invention.

By applying the two substances, hyaluronic acid and an aldehyde peptide, to the composition, it is possible to improve the coarsening and dryness of the skin, and at the same time, activate the cells of the skin tissue to improve wrinkles.

The effects of the present invention are not limited to those mentioned above, and other solutions not mentioned may be clearly understood by those skilled in the art from the following description.

1 is a graph showing the results of measurement of cytotoxicity in a fibroblast (Human Skin Fibroblast) against the composition of the present invention.
2 is a graph showing the concentration-dependent growth promoting activity in the production of procollagen type 1 in human keratinocytes according to the present invention.
3 is a graph showing the results of evaluating cell regeneration efficiency of keratinocytes (Human Keratinocyte) of the composition of the present invention.

The present invention has been completed on the basis of hyaluronic acid, a derivative thereof and a salt thereof, which are generally usable in cosmetics and pharmacologically acceptable salts, and an alchol-oligopeptide-1. Hyaluronic acid, its derivatives and salts thereof, To provide skin elasticity and compactness by binding to collagen, fibronectin and proteoglycan, and aloe-oligopeptide-1 is a cosmetic composition for improving or preventing skin wrinkles, .

The derivative of hyaluronic acid of the present invention is exemplified by hydrolyzed hyaluronic acid, and hyaluronic acid salt is exemplified by sodium hyaluronate .

The hyaluronic acid, the derivative thereof and the salt thereof may each contain 0.01 to 0.8% by weight, more preferably 0.1 to 0.5% by weight.

The other major component of the wrinkle improvement of the present invention may contain 0.0000001 to 0.00001% by weight, more preferably 0.0000005 to 0.001% by weight, of the rh-oilgopeotide-1.

Also, 0.01 to 0.1 wt.% Of ethylhexyl glycerin, which is effective for effectively suppressing the growth of substances that may cause skin conditioning and smell, to reduce circulating deodorizing action, improve the efficacy of preservative ingredients and reduce the amount of preservatives, To 0.1% by weight.

As the preservative of the cosmetic of the present invention, at least one of methylparaben, propylparaben, ethylparaben phenoxyethanol and 1,2-hexanediol can be selected and the content thereof is suitably added in accordance with the allowable standard amount.

The secondary moisturizing effect component used in the present invention may include propylene glycol, butylene glycol, caprylic glycol, glycerin, and the like, and one or more of these may be used in combination.

Hereinafter, embodiments of the present invention will be described in detail. However, the present invention is not limited thereto.

Example 1 of a cosmetic composition for improving or preventing skin wrinkles according to the present invention having the composition shown in Table 1 below is prepared.

Furtherance Composition ratio (% by weight) Purified water 87.006499 Sodium hyaluronate 0.518 Hydrolyzed hyaluronic acid 0.1 Hyaluronic Acid 0.1 Methylpropanediol 5.05 glycerin 5.05 1,2-hexanediol 2.028 lecithin 0.08 Ethylhexyl Glycerin 0.0505 Butylene glycol 0.008 Caprylic Glycol 0.008 Collagen 0.001 Al-H-oligopeptide-1 0.000001

[Experimental Example 1]

Evaluation of the stability of the composition

The composition of Example 1, Table 1, was subjected to MTT analysis in human skin fibroblasts ranging from 0.01 to 10% to evaluate cytotoxicity.

As shown in FIG. 1, up to a concentration of 2.5% promoted cell proliferation in a concentration-dependent manner, and cytotoxicity was observed at a concentration of 5% or more. Therefore, it can be seen that up to a concentration of 2.5% promotes the proliferation of the skin fibroblasts, thereby improving wrinkles.

[Experimental Example 2]

The composition of procollagen type 1 ( Procollagen  type 1) generation evaluation

In the toxicity evaluation of Experimental Example 1, the production of procollagen type 1 in human skin fibroblast was evaluated for 0.5, 1.9 and 2.5% of the compositions in Table 1, which are the stability concentration regions.

As shown in FIG. 2, when compared with the effect of concentration-dependent cell proliferation, by showing a higher level of procollagen production, it is effective for improving wrinkles by increasing not only cell proliferation effect but also procollagen production .

[Experimental Example 3]

Evaluation of cell regeneration effect of composition

The cell regeneration efficiency of keratinocytes (Human Keratinocyte) was evaluated under the composition concentration condition of Experimental Example 2.

As can be seen from FIG. 3, natural EGF showed excellent cell regeneration efficiency at a concentration of 10 ng / ml and showed a concentration-dependent cell regeneration effect. Thus, wrinkle and elasticity improvement It can be seen that there is an effect on

[Sensory Test Evaluation]

The composition and composition ratio of the other components were the same as those of the composition of Example 2, except that the composition of Example 1 and the composition of Example 1 were used in place of the purified water. The sensory test was carried out.

The natural extract solution of Example 2 was prepared by mixing purified water and an extract at a weight ratio of 4: 1, and the extract was prepared by mixing a bamboo leaf extract, a lotus root extract, a Hongxia seed extract, and a wood vinegar at a weight ratio of 1: 0.5: 0.5: 0.2 will be.

Here, the bamboo leaf extract is obtained by adding 10 parts by weight of bamboo leaf to 100 parts by weight of water, heating it for 3 hours and then filtering it. The lotus root extract is obtained by heating for 3 hours and then adding 20 parts by weight to 100 parts by weight of water. The vinegar solution was diluted 1/10 of the vinegar solution sold in the market. The extract of Hongxi seed was obtained by heating 10 parts by weight of powder obtained by pulverizing Hongsi seed into 100 parts by weight of water, heating it for 4 hours, and filtering.

Twenty panel members were selected and evaluated for their stickiness, degree of skin absorption, and preference for compositions of Example 1 and Example 2, and then each composition was used, followed by a 5-point scoring method (5: very good, 4: : Normal, 2: Bad, 1: Very bad). The results are summarized in Table 2 below.

Example 1 Example 2 Stickiness 4.2 4.6 Skin absorption 4.3 4.5 Overall likelihood 4.3 4.6

As can be seen from the above Table 2, when the natural extract of Example 2 was used as compared with Example 1 using ordinary purified water, it was confirmed that stickiness was less, skin absorption was good, and consumer preference increased remarkably.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and similarities. Accordingly, the scope of the present invention should be construed as being limited to the embodiments described, and it is intended that the scope of the present invention encompasses not only the following claims, but also equivalents thereto.

Claims (5)

Rh-Oligopeptide; And hyaluronic acid or derivatives thereof as an active ingredient.
The method according to claim 1,
Wherein the hyaluronic acid derivative is hydrolyzed hyaluronic acid. 2. The cosmetic composition according to claim 1, wherein the hyaluronic acid derivative is hydrolyzed hyaluronic acid.
The method according to claim 1,
Wherein the aloe-oligopeptide is contained in an amount of 0.0000001 to 0.00001 parts by weight based on 100 parts by weight of the cosmetic composition for improving skin wrinkles.
The method according to claim 1,
Wherein the hyaluronic acid or a derivative thereof is contained in an amount of 0.1 to 0.3 parts by weight based on 100 parts by weight of the total amount of the cosmetic composition for improving skin wrinkles.
The method according to claim 1,
Wherein the cosmetic composition has a formulation selected from the group consisting of lotion, milky lotion, cream, essence, cosmetic lotion, spray, gel, pack, sunscreen, makeup base, foundation, powder, make- Wherein the cosmetic composition is a cosmetic composition.

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