KR20180047952A - Functional composition comprising deer antlers derived stem cell culture medium - Google Patents
Functional composition comprising deer antlers derived stem cell culture medium Download PDFInfo
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- KR20180047952A KR20180047952A KR1020160144891A KR20160144891A KR20180047952A KR 20180047952 A KR20180047952 A KR 20180047952A KR 1020160144891 A KR1020160144891 A KR 1020160144891A KR 20160144891 A KR20160144891 A KR 20160144891A KR 20180047952 A KR20180047952 A KR 20180047952A
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- antler
- cell culture
- composition
- stem cell
- hair growth
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Abstract
Description
본 발명은 녹용 줄기세포 배양액을 포함하는 기능성 조성물에 관한 것으로, 보다 상세하게는 녹용 유래 줄기세포 배양액을 포함하며, 탈모 치료, 피부 개선 및 상처 치료 효과가 있는 기능성 조성물에 관한 것이다. The present invention relates to a functional composition comprising a deer antler stem cell culture liquid, and more particularly, to a functional composition containing a deer antler stem cell culture solution and having an effect of treating hair loss, skin improvement and wound healing.
녹용이란 사슴의 뿔이 딱딱하게 각질화되기 전에 잘라서 약으로 사용하는 것을 말하며 전통적으로 인삼과 더불어 가장 우수한 보양강장제로 한방에서 수침액으로 빈번히 사용되어 왔다. 따라서, 국내에서 사슴 사육의 목적은 주로 녹용 생산을 위한 것이고 녹용의 약용으로서의 효능은 한방에서 오래전부터 기록되어 있다. 동의보감, 본초비용, 본초강목, 의방유취, 본초정, 천금방, 종교본초강목, 약성론, 본초경소론 등에 따르면 신경쇠약, 피로회복, 성기능저하, 보혈, 빈혈, 원기회복, 폐결핵, 허리나 다리의 통증, 자궁출혈, 불면증 등 다양한 곳에 탁월한 효과를 가지고 있다는 기록이 되어있다. Deer antler is a drug used to cut deer 's horns before it is hardened. It has been traditionally used as the most excellent preservative tonic with ginseng as a soaking solution in oriental medicine. Therefore, the purpose of raising deer in Korea is mainly for the production of antler, and the efficacy of antler for medicinal use has long been recorded in oriental medicine. According to the Dongbokbo, the herb cost, the herb gangmok, the herb lavage, the primordial gland, the gangbang, the religious primordial gangbang, Pain, uterine bleeding, insomnia and has been recorded in various places have excellent effects.
이러한, 녹용의 주성분은 지질, 단백질, 탄수화물, 무기질 및 미량원소 등으로 구성되어 있으며 이런 성분들에 기인하여 녹용이 다양한 생리활성이 발현하는 것으로 받아 들여지고 있다. The major components of antler are composed of lipids, proteins, carbohydrates, minerals and trace elements, and it is considered that the antler contains various physiological activities due to these components.
한편, 녹용의 생리기능에 대한 연구는 국내 한의학분야에서 활발히 진행되어, 항피로효과, 면역활성 증가작용, 단백질 합성촉진작용, 내분비 기능촉진작용, 조혈작용, 면역활성 증가작용, 진통작용, 콜레스테롤 저하 작용, 간 기능 개선작용, 성장촉진작용 및 당뇨병치료효과 등이 보고되고 있다. On the other hand, research on the physiological function of antler has been actively conducted in the field of oriental medicine in Korea and has been actively carried out in the field of oriental medicine, and has been widely applied in the fields of anti-fatigue, immunologic activity increasing, protein synthesis promoting, endocrine function promoting, hematopoietic, , Liver function improving action, growth promoting action and diabetic therapeutic effect have been reported.
최근 연구에 따르면, 녹용 줄기세포는 녹용의 핵심 세포로서 녹용이 재생되는데 가장 중요한 역할을 한다. 녹용줄기세포의 경우 다양한 세포로의 분화가능성을 가지고 있다는 보고가 되어있다(비특허문헌 1).According to a recent study, deer antler cells are the key cell of antler and play the most important role in regenerating antler. It has been reported that antler stem cells have differentiation potential into various cells (Non-Patent Document 1).
최근 들어 인체 유래 줄기세포를 이용한 제품들은 유럽 등지에서 제품 판매가 금지되어 있으며, 높은 가격 등에 의해 판로가 적은 것이 사실이다. 이에 본 발명은 인체 유래 줄기세포를 대신하여 녹용과 녹용 줄기세포를 이용하여 상처 치료, 피부 개선 및 탈모 개선 제품을 개발하여 대량생산 공정에 도입하는 것이 최종 목적이다.In recent years, products using human-derived stem cells have been prohibited from being sold in Europe, and it is true that there are few outlets due to high prices. Accordingly, it is an object of the present invention to develop products for wound healing, skin improvement and hair loss improvement using antler and deer antler cells in place of human stem cells, and to introduce them into a mass production process.
본 발명은 상기와 같은 종래의 기술상의 문제점을 해결하기 위해 안출된 것으로, 본 발명의 일 목적은 녹용 유래 줄기세포 배양액 또는 녹용 유래 줄기세포 배양액 유래 엑소좀을 탈모의 예방 또는 치료에 사용할 수 있는 조성물과 그 제조방법을 제공하고자 한다.DISCLOSURE Technical Problem Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and it is an object of the present invention to provide a composition for preventing or treating hair loss, which can be used for preventing or treating hair loss derived from antler stem cell culture broth or stem- And a manufacturing method thereof.
본 발명의 다른 목적은 녹용 유래 줄기세포 배양액을 피부 미백, 보습, 주름 개선 및 노화 방지 등 피부 개선에 사용할 수 있는 조성물과 그 제조방법을 제공하고자 한다.Another object of the present invention is to provide a composition which can be used for improving skin such as skin whitening, moisturizing, wrinkle improvement and anti-aging, and a method for producing the composition.
본 발명의 또 다른 목적은 녹용 유래 줄기세포 배양액을 포함하는 상처 치료에 사용할 수 있는 조성물과 그 제조방법을 제공하고자 한다.It is still another object of the present invention to provide a composition for use in the treatment of wounds containing a stem cell culture solution derived from a deer antler, and a method for producing the same.
그러나, 본 발명이 이루고자 하는 기술적 과제는 이상에서 언급한 과제에 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 당 업계에서 통상의 지식을 가진 자에게 명확하게 이해될 수 있을 것이다.However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.
본 발명의 발명자들은 녹용 줄기세포 배양액 또는 녹용 줄기세포 배양액 유래 엑소좀이 발모 및 육모 효과가 뛰어나고, 피부 개선 및 상처 치료가 뛰어나며, 녹용 추출물을 혼합하여 사용하는 경우 상기 효과에 시너지 효과가 발생하는 발견하여 본 발명에 이르게 되었다. The inventors of the present invention have found out that exosome derived from a deer antler stem cell culture broth or deer stria cell culture broth is excellent in hair growth and hair growth and is excellent in skin improvement and wound healing and that a synergistic effect is obtained when the antler extract is mixed and used Thereby leading to the present invention.
이하, 본원에 기재된 다양한 구현예가 도면을 참조로 기재된다. 하기 설명에서, 본 발명의 완전한 이해를 위해서, 다양한 특이적 상세사항, 예컨대, 특이적 형태, 조성물 및 공정 등이 기재되어 있다. 그러나, 특정의 구현예는 이들 특이적 상세 사항 중 하나 이상 없이, 또는 다른 공지된 방법 및 형태와 함께 실행될 수 있다. 다른 예에서, 공지된 공정 및 제조 기술은 본 발명을 불필요하게 모호하게 하지 않게 하기 위해서, 특정의 상세사항으로 기재되지 않는다. "한 가지 구현예" 또는 "구현예"에 대한 본 명세서 전체를 통한 참조는 구현예와 결부되어 기재된 특별한 특징, 형태, 조성 또는 특성이 본 발명의 하나 이상의 구현예에 포함됨을 의미한다. 따라서, 본 명세서 전체에 걸친 다양한 위치에서 표현된 "한 가지 구현예에서" 또는 "구현예"의 상황은 반드시 본 발명의 동일한 구현예를 나타내지는 않는다. 추가로, 특별한 특징, 형태, 조성, 또는 특성은 하나 이상의 구현예에서 어떠한 적합한 방법으로 조합될 수 있다.Hereinafter, various embodiments described herein will be described with reference to the drawings. In the following description, for purposes of complete understanding of the present invention, various specific details are set forth, such as specific forms, compositions and processes, and the like. However, the specific embodiments may be practiced without one or more of these specific details, or with other known methods and forms. In other instances, well-known processes and techniques of manufacture are not described in any detail, in order not to unnecessarily obscure the present invention. Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, form, composition, or characteristic described in connection with the embodiment is included in one or more embodiments of the invention. Accordingly, the appearances of the phrase " in one embodiment "or" an embodiment "in various places throughout this specification are not necessarily indicative of the same embodiment of the invention. In addition, particular features, shapes, compositions, or characteristics may be combined in any suitable manner in one or more embodiments.
본 발명의 일 구현예에서, 녹용 줄기세포 배양액을 유효성분으로 포함하는 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물을 제공한다. 상기 구현예에서, 상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200㎍/㎖을 포함하는 배지에서 녹용유래줄기세포를 배양하여 제조된 것이며, 상기 조성물은 모낭형성을 촉진시키며, 상기 조성물은 Wnt 신호전달 경로 내 Wnt-3a 또는 Wnt-10b 유전자의 발현을 증가시키며, 상기 조성물은 중간엽줄기세포로부터 EGF, IGF, TGF, 및 VEGF로 구성된 군으로부터 선택된 어느 하나의 성장인자 분비를 촉진시키는 것인, 탈모 예방 또는 치료용, 또는 발모 촉진 또는 육모 촉진용 조성물을 제공한다.In one embodiment of the present invention, there is provided a composition for preventing or treating hair loss or promoting hair growth or hair growth comprising an antler stem cell culture liquid as an active ingredient. In the above embodiment, the deer antler cell culture solution is prepared by culturing the antler-derived stem cells in a medium containing 5 to 200 μg / ml of antler extract, the composition promoting hair follicle formation, Wherein the composition promotes the expression of Wnt-3a or Wnt-10b gene in the pathway, wherein the composition promotes secretion of any one growth factor selected from the group consisting of EGF, IGF, TGF, and VEGF from mesenchymal stem cells Preventing or treating hair, or promoting hair growth or promoting hair growth.
본 발명의 일 구현예에서, 녹용 줄기세포 배양액 유래 엑소좀을 유효성분으로 포함하는 탈모 예방 또는 치료용 또는 발모 또는 육모 촉진용 조성물을 제공한다. 상기 구현예에서, 상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용유래 줄기세포를 배양하여 제조된 것인, 탈모 예방 또는 치료용, 또는 발모 촉진 또는 육모 촉진용 조성물을 제공한다.In one embodiment of the present invention, there is provided a composition for preventing or treating hair loss or for promoting hair growth or hair growth comprising exosome derived from an antler stem cell culture liquid as an active ingredient. In the above embodiment, the deer antler cell culture solution is prepared by culturing the antler stem cells in a medium containing 5 to 200 占 퐂 / ml of antler extract, for preventing or treating hair loss, or for promoting hair growth or hair growth .
본 발명의 일 구현예에서, 녹용 줄기세포 배양액을 유효성분으로 포함하는 상처 치료용 약학적 조성물을 제공한다. 상기 구현예에서, 상기 상처는 피부에 있는 것으로, 베임(cut), 절개(incisions), 찰과상(abrasions), 열상 또는 찢김(lacerations), 골절(fractions), 타박(contusions), 화상(burns), 또는 절단(amputations)으로 구성된 군으로부터 선택된 어느 하나에 의한 상처이고, 상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200㎍/㎖을 포함하는 배지에서 녹용유래 줄기세포를 배양하여 제조되며, 상기 조성물은 중간엽줄기세포로부터 EGF, IGF, TGF, 및 VEGF로 구성된 군으로부터 선택된 어느 하나의 성장인자 분비를 촉진시키는 것인, 상처 치료용 약학 조성물을 제공한다.In one embodiment of the present invention, there is provided a pharmaceutical composition for treating a wound comprising an antler stem cell culture solution as an active ingredient. In such an embodiment, the wound is on the skin, and may include cuts, incisions, abrasions, lacerations, fractures, contusions, burns, Or amputations, and the deer antler culture is prepared by culturing antler-derived stem cells in a medium containing 5 to 200 μg / ml of antler extract, Wherein the growth factor promotes secretion of growth factors selected from the group consisting of EGF, IGF, TGF, and VEGF from the leaf stem cells.
본 발명의 일 구현예에서, 상기 구현예에 개시된 조성물을 개체에 투여하는 단계를 포함하는 사람이 아닌 포유동물의 상처를 치료하는 방법을 제공한다.In one embodiment of the invention, there is provided a method of treating a wound in a non-human mammal comprising administering to the subject a composition as disclosed in the embodiment.
본 발명의 일 구현예에서, 녹용 줄기세포 배양액을 유효성분으로 포함하는 피부주름 또는 피부노화 예방 또는 개선용 화장료 조성물을 제공한다. 상기 구현예에서, 상기 조성물은 인체피부표피세포 또는 인체진피섬유아세포의 증식을 증가시키며, 상기 화장료는 화장수, 에센스, 로션, 크림, 팩, 팩, 젤, 연고, 파우더, 비누, 클렌징폼, 바디클린저, 스킨소프너, 스킨토너, 및 분무제로 구성된 군으로부터 선택된 어느 하나인 피부주름 또는 피부노화 예방 또는 개선용 화장료 조성물을 제공한다.In one embodiment of the present invention, there is provided a cosmetic composition for preventing or improving skin wrinkles or skin aging comprising an antler stem cell culture liquid as an active ingredient. In this embodiment, the composition increases the proliferation of human skin epidermal cells or human dermal fibroblasts. The cosmetic composition may be in the form of a lotion, essence, lotion, cream, pack, pack, gel, ointment, powder, soap, cleansing foam, Wherein the cosmetic composition is any one selected from the group consisting of a cleanser, a skin softener, a skin toner, and a spraying agent.
본 발명의 일 구현예에서, 녹용 줄기세포 배양액 또는 녹용 줄기세포 배양액 유래 엑소좀을 유효성분으로 포함하는 탈모 개선용, 또는 발모 또는 육모 촉진용 식품 조성물을 제공한다. 상기 구현예에서, 상기 배양액은 녹용 추출물 5 내지 200㎍/㎖을 포함하는 배지에서 인간지방유래줄기세포를 배양하여 제조된 것인, 탈모 개선용, 또는 발모 또는 육모 촉진용 식품 조성물을 제공한다. In one embodiment of the present invention, there is provided a food composition for enhancing hair loss or for promoting hair growth or hair growth comprising an antler stem cell culture solution or exosome derived from a deer antler culture medium as an active ingredient. In this embodiment, the culture solution is prepared by culturing human adipose-derived stem cells in a medium containing 5 to 200 占 퐂 / ml of antler extract, wherein the composition for improving hair loss or promoting hair growth or hair growth is provided.
본 발명의 일 구현예에서 녹용 줄기세포 배양액을 유효성분으로 포함하는 피부주름 또는 피부노화 개선용 식품 조성물을 제공한다. 상기 구현예에서, 상기 배양액은 녹용 추출물 5 내지 200㎍/㎖을 포함하는 배지에서 녹용유래 줄기세포를 배양하여 제조된 것인, 피부주름 또는 피부노화 개선용 식품 조성물을 제공한다. In one embodiment of the present invention, there is provided a food composition for improving skin wrinkles or skin aging comprising an antler stem cell culture liquid as an active ingredient. In this embodiment, the culture solution is prepared by culturing the stem cells of antler in a medium containing 5 to 200 μg / ml of antler extract, wherein the composition is used for improving skin wrinkles or skin aging.
본 발명 내 특별한 정의가 없으면 본 명세서에 사용된 모든 과학적 및 기술적인 용어는 본 발명이 속하는 기술분야에서 당 업자에 의하여 통상적으로 이해되는 것과 동일한 의미를 가진다.Unless defined otherwise within the present invention, all scientific and technical terms used herein have the same meaning as commonly understood by one of skill in the art to which this invention belongs.
본 발명의 녹용 줄기세포는 바람직하게는 녹용의 생장점이 몰려 있는 녹용 상단의 말단에서 아래로 1~3㎝ 되는 부분인 mesenchemy 부분 또는 팁 부분으로부터 분리될 수 있으나, 녹용 줄기세포를 분리할 수 있다면 분골, 상대 등 녹용의 모든 부위로부터 분리될 수 있다. The deer antler cells of the present invention can be separated from the mesenchemy portion or the tip portion, which is a
본 발명에서 “녹용 추출물”은 녹용(cervi parvum corun, 사슴 뿔) 유래의 추출물로서 강장작용, 보혈작용, 강정작용, 진통작용, 조혈작용, 생장발육촉진작용, 심부전증 치료작용, 기능항진작용, 피부세포재생, 피로회복, 신체활력증강 및 신장의 이뇨기능강화 효능을 나타내는 유효성분을 포함하며, 바람직하게는 녹용을 분쇄한 후 물을 용매로 하여 추출하여 제조되지만 녹용의 유효성분을 포함한다면, 가열 추출, 화학적 추출, 물리적 추출 등의 방법으로 제조될 수 있다.In the present invention, the " antler extract " is an extract derived from cervi parvum corun (antlers), which is used as a therapeutic agent for skin, blood, gangjeong, analgesic, hematopoietic, The present invention relates to an active ingredient which exhibits an effect of enhancing diuretic function of cell regeneration, fatigue recovery, body vitality enhancement and kidney function, and is preferably prepared by pulverizing antler seed and extracting it with water as a solvent. However, Extraction, chemical extraction, physical extraction, and the like.
본 발명에서 “엑소좀(exosome)”은 엔도좀(endosome)에서 기원한 미세소포체(microvesicle)로서, 여러 종류의 세포들로부터 분비되는 막 구조의 소포체(vesicle)이며, 단백질 및 유전적 정보를 포함하고 있어 세포 밖으로 유전정보를 포함한 다양한 신호를 다른 세포에 전달하여 발달과정, 증식, 분화, 면역조절, 염증, 혈액응고, 혈관생성, 세포와 환경 간의 상호작용 및 다양한 질병 진행 등에 관여하는 것으로 알려져 있다.In the present invention, "exosome" is a microvesicle originated from the endosome. It is a vesicle of membrane structure secreted from various kinds of cells. It contains protein and genetic information. And is known to be involved in development processes, proliferation, differentiation, immune regulation, inflammation, blood coagulation, angiogenesis, interaction between cells and environment, and various disease progression by transferring various signals including genetic information to other cells .
본 발명에서 "탈모"는 모발이 완전히 두피 밖으로 빠져나오게 되는 형상을 의미한다. 본 발명에서 "발모"는 두피에서 모발이 나는 것을 의미하고, "육모"는 모발의 길이가 길어지는 것(모발 성장)을 의미하며 당 업계의 기술 분야에 사용되는 다른 용어인 "양모"와 동일한 의미로 사용된다. 탈모가 진행되고 있는 사람은 성장기가 짧아지고 휴지기가 긴 모발주기를 갖게 되는데, 본 발명에 따른 조성물은 모발을 휴지기에서 성장기로 전환시키고, 피부조직에서 발모 및 양모와 관련된 세포의 증식 또는 신호전달 경로를 활성화시켜서 탈모를 예방, 개선 또는 치료 효과가 있음을 확인하였다. In the present invention, "hair loss" means a shape in which the hair completely comes out from the scalp. In the present invention, "hair growth" means that the hair grows in the scalp, "hair growth" means that hair grows longer (hair growth), and is similar to other terms used in the art It is used as a meaning. A person having hair loss is shortened in growth period and has a long hair cycle. The composition according to the present invention changes the hair from a resting period to a growing period, and the hair growth and wool-related cell proliferation or signal transduction pathway To prevent, ameliorate or treat hair loss.
본 발명에서 "상처(wound)"는 조직이 잘려지거나(cut), 찢어지거나(torn), 부서지거나(broken), 타거나(burned), 또는 외상을 입거나(traumatized), 또는 이러한 손상을 유발하는 장애 또는 질환으로부터 발생한 인체에 대한 손상(injury)를 의미한다. 상기 상처는 표면이 개방된 상처 또는 표면이 개방되지 않은 상처일 수 있다. 상기 상처는 병변(lesion), 헌데(sore), 괴사(necrosis), 및 궤양(ulcer)을 포함할 수 있다. 상기 상처의 일 예는 피부의 표피; 진피; 표피 및 진피; 또는 표피, 진피 및 피하지방층이 손상된 상처일 수 있다.In the present invention a "wound" means that the tissue is cut, torn, broken, burned, traumatized, Or injury to the human body caused by the disorder or disease. The wound may be a wound whose surface is open or a wound whose surface is not open. The wound may include lesions, sores, necrosis, and ulcers. Examples of such wounds include epidermis of the skin; dermis; Epidermis and dermis; Or the damaged skin of the epidermis, dermis and subcutaneous fat layer.
피부는 외부로부터 신체를 보호하는 방어막으로서의 역할을 한다. 피부에 상처가 생기면 생체의 자연 치유 작용에 의해 상처자리를 혈액이 채우게 되고 혈소판의 과립감소와 하게만 인자(Hageman factor)의 활성화가 시작되어 상처 치유 과정이 진행된다. 상처 치료 및/또는 치유는 크게 3단계로 진행된다. 첫 번째 단계는 외상 부위에서의 염증을 특징으로 하는 염증기(inflammatory phase)이다. 두 번째 단계는 증식기로서 상피화, 혈관형성, 육아 조직 형성 및 콜라겐 침착을 특징으로 한다. 세 번째 단계는 섬유아세포가 콜라겐으로 분화하는 성숙기이다. 상처 치유 과정에서 상처를 신속하게 치료할 뿐만 아니라 부작용이나 흉터 없이 치료하는 것이 중요하므로, 상기 효능을 갖는 물질을 찾고자 하는 노력이 지속적으로 진행 중에 있다. Skin acts as a shield to protect the body from the outside. When a wound occurs in the skin, the wound is filled with blood by the natural healing action of the living body, and the granule of the platelet is reduced and the activation of the factor (Hageman factor) starts and the wound healing process proceeds. Wound healing and / or healing generally progresses to three stages. The first step is an inflammatory phase characterized by inflammation at the traumatic site. The second stage is characterized by epithelialization, angiogenesis, granulation formation and collagen deposition as proliferators. The third stage is the maturation phase in which fibroblasts differentiate into collagen. Since it is important not only to treat wounds quickly during wound healing, but also to treat them without side effects or scars, efforts are constantly being made to find those substances having the above-mentioned effects.
본 발명에 의하여 제공되는 "치료"는 자연 치유에 비하여 단축된 시간에 상처가 치유되는 것일 수 있다. 상기 치료는 상처의 개선 및/또는 완화를 포함할 수 있다. 상기 치료는 상처로부터 유발되는 손상된 조직의 치유 및/또는 재생을 의미할 수 있다. 상기 치료는 상처로부터 유발되는 손상된 조직의 치유 및/또는 재생을 의미할 수 있다. 상기 상처 치료는 피부 재생의 의미를 포함할 수 있다. 또한, "상처의 개선"은 상처의 정도를 낮추어주거나 완화시키는 용도를 포함할 수 있다. The "treatment" provided by the present invention may be to heal the wound at a reduced time compared to natural healing. The treatment may include improvement and / or alleviation of the wound. Such treatment may mean healing and / or regeneration of damaged tissue caused by a wound. Such treatment may mean healing and / or regeneration of damaged tissue caused by a wound. The wound treatment may include the meaning of skin regeneration. In addition, "improvement of wound" may include the use of lowering or alleviating the degree of wound.
본 발명에서 Wnt β-catenin 신호전달계는 발모 및 상처 치유에도 관련이 있다. Wnt β-catenin 신호전달계는 모낭의 형성을 촉진하고(비특허문헌 2), 모발주기의 성장기 동안 발현하는 유전자들을 유지하고 활성화하는데 중요한 역할을 하며(비특허문헌 3), 줄기세포로부터 케라티노사이트로의 분화를 촉진한다고 보고되어 있다(비특허문헌 4). 또한, Wnt/β-카테닌 신호전달계는 TGF-β 신호전달계의 활성화를 통해 상처 치유를 강화하고(비특허문헌 5), 상처 치유 과정에서 중요한 조절자로 알려진 근섬유아세포의 형성을 촉진한다고 보고되어 있다(비특허문헌 6).In the present invention, the Wnt [beta] -catenin signal transduction system is also involved in hair growth and wound healing. The Wnt β-catenin signal transduction system promotes the formation of hair follicles (Non-Patent Document 2) and plays an important role in maintaining and activating genes expressed during the hair cycle growing period (Non-Patent Document 3) (Non-Patent Document 4). It has also been reported that the Wnt / beta -catenin signaling system enhances wound healing through activation of the TGF-beta signaling system (Non-Patent Document 5) and promotes the formation of fibroblast cells, which are known to be important regulators in the wound healing process Non-Patent Document 6).
본 발명에서 "중합효소연쇄반응(PCR, Polymerase Chain Reaction)"은 아주 적은 양의 DNA 만으로도 특정 부위의 DNA 염기서열을 기하급수적으로 증폭시킬 수 있는 간단하고 편리한 방법으로, 증폭하고자 하는 DNA에 특이적으로 결합하여 고온에서도 안정한 Taq DNA 중합효소를 사용하여 변성(denaturation), 어닐링(annealing), 연장(extension)의 과정을 반복적으로 실행함으로써 특정 DNA를 증폭시키게 된다. PCR을 이용한 목적 유전자의 검출은 유전자를 인식하는 프라이머를 이용하여 이를 증폭 및 확인하는 것이다.In the present invention, "PCR (Polymerase Chain Reaction)" is a simple and convenient method for exponentially amplifying a DNA sequence at a specific site using only a small amount of DNA, And DNA amplification is performed by repeatedly performing denaturation, annealing, and extension using a stable Taq DNA polymerase at high temperatures. The detection of the target gene using PCR is performed by amplifying and confirming the gene using a primer recognizing the gene.
본 발명에서 "리얼타임(real-time) PCR"이란 형광물질을 PCR 기법에 응용한 것으로, 반응 중 검체 내에 존재하는 표적 유전자의 증폭과 함께 형광물질의 발광(emission) 정도를 실시간으로 검출하고 정량 분석하여 표적 유전자의 증폭 유무 및 그 양상을 신속하고 정확하게 분석할 수 있는 방법이다. 전통적인 PCR 기법에 비해, PCR 증폭 산물을 분석하기 위한 gel 전기영동, DNA-DNA 혼성화, 서열분석 같은 추가적인 실험을 필요로 하지 않는다는 장점이 있다.The term "real-time PCR" used in the present invention refers to the application of a fluorescent substance to a PCR technique. In the reaction, amplification of a target gene existing in a specimen during a reaction is detected, And analyze the amplification of the target gene and its aspects quickly and accurately. Compared to traditional PCR techniques, it does not require additional experiments such as gel electrophoresis, DNA-DNA hybridization, and sequencing to analyze PCR amplification products.
본 발명에서 real-time PCR을 이용한 정량은 일반적으로 증폭반응 개시 시의 주형 DNA 양을 정량하기 위한, PCR을 이용한 일련의 반응을 의미한다. 정량적 PCR에는 기준이 되는 표준시료를 사용하는 내부 표준법과 증폭반응에 있어서 경쟁하는 분자를 사용하는 경쟁법이 포함된다. 또한, 각 서열의 분자 수를 정확하고 간편하게 결정하기 위해서 표준시료라고 불리는 기준이 되는 주형 DNA 서열을 이용한 PCR 방법을 사용하고, 반응 중 임의의 시점에서 반응의 진행상황, 즉 증폭의 정도를 확인한다.In the present invention, quantification using real-time PCR generally means a series of reactions using PCR to quantify the amount of template DNA at the start of the amplification reaction. Quantitative PCR includes internal standard methods using standard reference standards and competitive methods using molecules competing in amplification reactions. In order to accurately and easily determine the number of molecules of each sequence, a PCR method using a template DNA sequence as a standard called a standard sample is used, and the progress of the reaction, that is, the degree of amplification is checked at any time during the reaction .
본 발명에서 "줄기세포(stem cell)"란 자기 복제 능력을 가지면서 두 개 이상의 세포로 분화하는 능력을 갖는 세포로, 분화능력에 따라 만능 줄기세포(totipotent stem cell), 전분화능 줄기세포(pluripotent stem cell) 및 다분화능 줄기세포(multipotent stem cell)로 분류할 수 있다. The term "stem cell" in the present invention refers to a cell capable of self-replicating and capable of differentiating into two or more cells. According to the differentiation ability, pluripotent stem cells, pluripotent stem cells, stem cells) and multipotent stem cells (multipotent stem cells).
본 발명에서 "마커"란 줄기세포의 존재 확인에 이용될 수 있는 물질로서 줄기세포의 형성 단계에서 그 발현이 증가(상향조절), 감소(하향조절) 또는 변형된 형태의 발현을 보이는 물질이며, 상기 마커들은 줄기세포의 존재를 확인할 수 있는 표지자로서 사용될 수 있다.The term "marker" in the present invention refers to a substance which can be used for confirming the presence of stem cells and exhibits an expression (up-regulation), decrease (down regulation) The markers can be used as markers to confirm the presence of stem cells.
본 발명에서 "항체"란 단백질 또는 펩티드 분자의 항원성 부위에 특이적으로 결합할 수 있는 단백질성 분자를 의미한다."Antibody" in the present invention means a proteinaceous molecule capable of specifically binding to an antigenic site of a protein or peptide molecule.
본 발명에서 "면역염색법"은 세포와 조직에 발현되는 표적 인자에 특이적으로 결합하는 항체를 이용하여 단백질 발현 정보를 얻는 방법에 관한 것으로, 항체에 형광 물질을 결합시켜 표적 인자의 발현량을 관찰할 수 있으며 표적 인자의 발현에 대한 정량적 및 정성적 분석도 가능하다."Immunostaining method" in the present invention relates to a method for obtaining protein expression information by using an antibody that specifically binds to a target molecule expressed in cells and tissues. In this method, a fluorescent substance is bound to an antibody, Quantitative and qualitative analysis of the expression of target factors is possible.
본 발명의 약제학적 조성물은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있는 방법에 따라, 약제학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다. 이때, 제형은 오일 또는 수성 매질 중의 용액, 현탁액, 시럽제 또는 유화액 형태이거나 엑스제, 산제, 분말제, 과립제, 정제 또는 캅셀제 형태일 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수 있다. The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. Here, the formulations may be in the form of solutions, suspensions, syrups or emulsions in oils or aqueous media, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.
본 발명의 화장료 조성물에 포함되는 성분은 유효성분으로서 녹용 유래 줄기세포 배양액 또는 이의 약제학적으로 허용 가능한 염 이외의 화장품 조성물에 통상적으로 이용되는 성분들을 포함하는데, 예컨대 항산화제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 및 담체를 포함한다. The ingredients contained in the cosmetic composition of the present invention include the ingredients commonly used in cosmetic compositions other than the antler family stem cell culture solution or its pharmaceutically acceptable salt as the active ingredient, for example, antioxidants, stabilizers, Vitamins, pigments and flavoring agents, and carriers.
본 발명에서 화장료 조성물은 당 업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 화장수, 에센스, 로션, 크림, 팩, 팩, 젤, 연고, 파우더, 비누, 클렌징폼, 바디클린저, 스킨소프너, 스킨토너, 분무제, 용액, 현탁액, 유탁액, 페이스트, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 및 왁스 파운데이션 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다.The cosmetic composition according to the present invention may be prepared in any form conventionally produced in the art and may be in the form of a lotion such as lotion, essence, lotion, cream, pack, pack, gel, ointment, powder, soap, cleansing foam, But are not limited to, emulsions, creams, skin softeners, skin toners, sprays, solutions, suspensions, emulsions, pastes, surfactant-containing cleansing, oils, powder foundations, emulsion foundations and wax foundations .
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌 글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수있다.In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
본 발명의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.
본 발명의 조성물은 비경구 투여 제형으로 제형화될 수 있다. 비경구 투여 제형은 주사제, 또는 피부외용제일 수 있다. 피부 외용제는 크림, 겔, 연고, 피부 유화제, 피부 현탁액, 경피전달성 패치, 약물 함유 붕대, 로션, 또는 그 조합일 수 있다. 상기 피부 외용제는 통상 화장품이나 의약품 등의 피부 외용제에 사용되는 성분, 예를 들어 수성성분, 유성성분, 분말성분, 알코올류, 보습제, 중점제, 자외선흡수제, 미백제, 방부제, 산화방지제, 계면활성제, 향료, 색제, 각종 피부 영양제, 또는 이들의 조합과 필요에 따라서 적절하게 배합될 수 잇다. 상기 피부외용제는, 에데트산이나트륨, 에데트산삼나트륨, 시트르산나트륨, 폴리인산나트륨, 메타인산나트륨, 글루콘산 등의 금속봉쇄제, 카페인, 탄닌, 벨라파밀, 감초추출물, 글라블리딘, 칼린의 과실의 열수추출물, 각종 생약, 아세트산토코페롤, 글리틸리틴산, 트라넥삼산 및 그 유도체 또는 그 염등의 약제, 비타민 C, 아스코르브산 인산마그네슘, 아스코르브산글루코시드, 알부틴, 코지산, 글루코스, 프룩토스, 트레할로스 등의 당류 등도 적절하게 배합할 수 있다.The composition of the present invention can be formulated into a parenteral dosage form. The parenteral dosage form may be an injection or an external preparation for skin. The external skin preparation may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal patch, a drug-containing bandage, a lotion, or a combination thereof. The external preparation for skin may be a cosmetic product or a medicament for use in a composition for external use for skin such as an aqueous component, an oily component, a powder component, an alcohol, a moisturizer, a thickening agent, an ultraviolet absorber, a whitening agent, an antiseptic, Flavorings, coloring agents, various skin nutrients, or combinations thereof, and may be appropriately blended as required. The external preparation for skin may be a metal blocker such as sodium edetate, sodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate or gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridine, Vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, fructose, fructose and other herbal medicines, various herbal medicines, tocopherol acetate, glycyrrhizic acid, Sugars such as trehalose and the like can also be appropriately compounded.
본 발명의 또 다른 양태에 따르면, 본 발명은 녹용 유래 줄기세포 배양액 또는 이의 약제학적으로 허용 가능한 염을 유효성분으로 포함하는 탈모 개선용, 또는 발모 또는 육모 촉진용 기능성 식품 조성물 또는 식품 조성물을 제공한다.According to another aspect of the present invention, there is provided a functional food composition or food composition for enhancing hair loss or promoting hair growth or hair growth comprising an antler-derived stem cell culture liquid or a pharmaceutically acceptable salt thereof as an active ingredient .
본 발명의 녹용 유래 줄기세포 배양액을 포함하는 조성물이 기능성 식품 조성물 또는 식품 조성물로 제조되는 경우, 유효성분으로서 녹용 줄기세포 배양액 또는 이의 약제학적으로 허용 가능한 염뿐만 아니라, 기능성 식품 또는 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 포함한다. 상술한 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트 린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제[타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등]) 및 합성 향미제(사카린, 아스 파르탐 등)를 사용할 수 있다.When the composition comprising the antler-derived stem cell culture liquid of the present invention is prepared from a functional food composition or a food composition, it is preferable that the antler stem cell culture liquid or its pharmaceutically acceptable salt is used as an active ingredient, And includes, for example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.
본 발명의 기능성 식품 또는 식품 조성물이 드링크제로 제조되는 경우에는 본 발명의 유효성분인 녹용 줄기세포 배양액 또는 이의 약제학적으로 허용 가능한 염 이외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 두충 추출액, 대추 추출액 또는 감초 추출액 등을 추가로 포함시킬 수 있다.When the functional food or the food composition of the present invention is prepared by a drink, the composition of the present invention, which is the active ingredient of the present invention, or a pharmaceutically acceptable salt thereof, may contain citric acid, liquid fructose, sugar, glucose, acetic acid, Extract, jujube extract or licorice extract, and the like.
본 발명의 녹용 줄기세포 배양액은 피부 세포의 증식 촉진에 의한 피부 재생 또는 상처 치유 효과가 우수하다. 또한, 모유두세포의 Wnt 신호전달 경로 내 모낭형성 유전자의 발현 증가 및 모유두 세포의 증식 촉진에 의한 탈모 개선 효과가 우수하다. The deer antler cells culture solution of the present invention is excellent in skin regeneration or wound healing effect by promoting proliferation of skin cells. In addition, the expression of the hair follicle forming gene in the Wnt signaling pathway of the dermal papilla cell is enhanced and the hair loss improving effect is enhanced by promoting the proliferation of the dermal papilla cell.
따라서, 본 발명의 녹용 줄기세포 배양액을 사용하면 피부 주름 또는 상처를 효과적으로 치료 또는 개선할 수 있고, 탈모 방지 및 개선 또는 발모 촉진용 조성물을 제조할 수 있다.Accordingly, the use of the deer antler cells culture solution of the present invention can effectively treat or ameliorate skin wrinkles or wounds, and can provide a composition for preventing and treating hair loss, or for promoting hair growth.
도 1은 본 발명에 따른 녹용 줄기세포 배양액 제조시 사용한 녹용 유래의 줄기세포를 확인한 결과에 관한 것이다.
도 2는 본 발명에 따른 녹용 줄기세포 배양액에서 분리한 엑소좀을 분석한 결과에 관한 것이다.
도 3는 본 발명에 따른 녹용 줄기세포 배양액의 피부재생 세포의 증식에 대한 결과를 나타낸 것이다.
도 4는 본 발명에 따른 녹용 줄기세포 배양액의 상처 치유 효과를 나타낸 것이다.
도 5는 본 발명에 따른 녹용 줄기세포 배양액의 모유두세포 증식 촉진 효과를 나타낸 것이다.
도 6은 본 발명에 따른 녹용 줄기세포 배양액 유래 엑소좀의 모유두세포 내 Wnt 신호전달 유전자의 발현에 대한 효과를 나타낸 것이다.
도 7은 본 발명에 따른 녹용 줄기세포 배양액의 모유두세포 내 Wnt 신호전달 유전자의 발현에 대한 효과를 나타낸 것이다.
도 8은 본 발명에 따른 녹용 줄기세포 배양액의 인체지방유래 줄기세포에 대한 성장인자 생산에서 측분비(paracrine) 증진 효과를 나타낸 것이다.FIG. 1 is a graph showing the results of examining the stem cells derived from antler used in the preparation of the deer antler cell culture according to the present invention.
2 shows the results of analysis of exosomes isolated from the antler stem cell culture solution according to the present invention.
FIG. 3 shows the results of proliferation of skin regenerating cells of the antler stem cell culture solution according to the present invention.
4 shows the wound healing effect of the antler stem cell culture solution according to the present invention.
FIG. 5 shows the effect of stimulating the proliferation of dermal papilla cells in the deer antler cells culture according to the present invention.
6 shows the effect of the exosomes derived from the antler stem cell culture solution on the expression of the Wnt signal transduction gene in the dermal papilla cell according to the present invention.
FIG. 7 shows the effect of the antler stem cell culture solution according to the present invention on the expression of the Wnt signal transduction gene in the dermal papilla cell.
FIG. 8 shows the effect of enhancing paracrine in the production of growth factors for stem cells derived from human adipose by the deer antler culture according to the present invention.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당 업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .
녹용 추출물 제조Manufacture of antler extract
녹용 추출물의 제조는 유기용매나 고온 고압 추출이 아닌 물질의 활성을 높이기 위한 수추출을 바탕으로 진행한다. 녹용을 두께 1~2㎝ 정도로 자른 후 자른 녹용은 약사발, 균질기(homogenizer), 파쇄기 등을 이용하여 잘게 파쇄한다. 이후 그램당 2배의 volume에 달하는 PBS를 넣어준다. 세이커(Shaker) 및 교반기(stirrer)를 사용하여 상온에서 2~4시간 동안 반응시킨다. 이후 4℃에서 하룻밤 동안(overnight) 방치하여 유효 물질을 용출시킨다. 용출된 용액은 상층액만 가져와 2000rpm 에서 세포 및 기타 물질을 제거한다. 제거된 용액을 이용하여 0.45, 0.22㎛ 필터(filter)를 통하여 순차적으로 여과시켜 준다. 이후 추출물 내 단백질 양을 확인하고, 이후 실험에서 사용되는 녹용 추출물의 농도는 측정된 단백질의 양으로 실험을 진행하였다.The production of the antler extract proceeds based on water extraction to enhance the activity of the substance, not the organic solvent or high temperature and high pressure extraction. Deer antler is cut to a thickness of 1 to 2 cm, and the antler is finely crushed using a doctor's knife, a homogenizer, and a crusher. Then add 2 volumes of PBS per gram. The reaction is carried out at room temperature for 2 to 4 hours using a shaker and a stirrer. After that, it is left overnight at 4 ° C to elute the active substance. The eluted solution takes only the supernatant and removes cells and other substances at 2000 rpm. The filtered solution is filtered sequentially through a 0.45, 0.22 μm filter. After that, the amount of protein in the extract was confirmed, and then the concentration of the antler extract used in the experiment was experimented with the amount of protein measured.
녹용 줄기세포 배양액 및 녹용 줄기세포 배양액 유래 Origin of antler stem cell culture and deer stem cell culture 엑소좀Exosome 제조 Produce
2.1 녹용 줄기세포 분리 및 확인2.1 Isolation and identification of antler stem cells
본 발명에서 성체 녹용 줄기세포를 분리하기 위하여, 3~7세의 건강하고 번식 적령기이며, 연간 4킬로그램 이상의 녹용을 생산하는 우량종 숫사슴을 선택하여 녹용 조직의 적출을 먼저 실시하였다. 적출된 숫사슴의 녹용 윗 말단에서 아래로 1~3cm 되는 mesenchemy 부분에 대하여, 멸균된 수술 도구(수술용 가위 및 칼)를 이용하여 녹용 조직을 분리하였다. 이후 콜라게나제(collagenase)를 사용하여 녹용 조직에 대한 화학적 분해를 실시하였다. 우선, 콜라게나제 및 배양 배지(DMEM)에 혼합하여 0.4㎎/㎖ 농도로 제조하였다. 준비된 콜라게나제-DMEM 배지를 녹용 조직에 처리하여 34~37℃, 5% CO2의 배양기에서 6시간 동안 배양하였다. 녹용 줄기세포가 포함된 배지는 1200rpm에서 10분간 원심분리하여 부유세포 및 콜라게나제를 포함하는 불순물을 제거하였다. 이와 같이 불특정 녹용 조직으로부터 추출된 3 종류의 녹용 유래 줄기세포(도 1 내 1, 2 및 3)는 5%의 FBS(우태아혈청; fetal bovine serum)를 함유하는 세포배양용 배지가 담긴 플라스크에 접종하였고, 34~37℃, 5% CO2의 배양기에서 24~72시간 동안 배양하여 배양용기 표면에 부착시켰고, 세포단층을 생성하는 세포만을 계대 배양하였다. 이후, STRO-1 및 CD90에 특이적으로 결합하는 항체를 이용한 면역염색법을 통하여 중간엽 줄기세포 마커인 STRO-1 및 CD90을 확인하여 녹용 유래 줄기세포임을 확인하였다(도 1).In order to isolate adult deer antler cells in the present invention, a deer antler, which is a healthy, reproductive aged male aged 3 to 7 years old and produces 4 kilograms or more perennial antler, was selected to extract the antler tissue. For the
2.2 녹용 줄기세포 배양액 제조2.2 Manufacture of antler stem cell culture
녹용 줄기세포 배양액을 제조하기 위하여 녹용으로부터 분리 및 배양된 줄기세포를 FBS(우태아혈청; fetal bovine serum)를 함유하지 않은 세포배양용 배지가 담긴 플라스크에 새롭게 접종하였고, 실시예 1에서 제조한 녹용 추출물을 농도별로 첨가하여 34~37℃, 5% CO2의 배양기에서 24~72시간 배양하여, 줄기세포 배양물을 획득하였고, 획득된 배양물은 0.22㎛ 필터를 통하여 여과시켜 녹용 줄기세포 배양액을 제조하였다.In order to prepare a deer antler culture, stem cells separated and cultured from antler were inoculated in a flask containing a cell culture medium not containing FBS (fetal bovine serum) The extracts were added at different concentrations and cultured for 24 to 72 hours in an incubator at 34 to 37 ° C and 5% CO 2 to obtain stem cell cultures. The obtained cultures were filtered through a 0.22 μm filter to obtain an antler stem cell culture .
2.3 엑소좀 분석2.3 Exosome analysis
녹용 줄기세포 배양액으로부터 엑소좀을 분리하기 위하여, ExoQuick exosome precipitation solution(SBI System Biosciences Cat No. EXOQ50A-1)을 이용하여 제조사의 매뉴얼에 기재된 방법에 따라 엑소좀을 수득하였다. 수득한 엑소좀은 인산완충식염수(PBS, Phosphate-Buffered Saline)을 사용하여 2회 세척하였다. 세척한 엑소좀 침전물은 10,000×g에서 60분간 원심 분리한 후 PBS에 재현탁하였다.In order to isolate exosome from the deer antler stem cell culture, exosomes were obtained according to the method described in the manufacturer's manual using ExoQuick exosome precipitation solution (SBI System Biosciences Cat No. EXOQ50A-1). The resulting exosomes were washed twice with PBS (Phosphate-Buffered Saline). The washed exosome precipitate was centrifuged at 10,000 × g for 60 minutes and resuspended in PBS.
상기 녹용 줄기세포 배양액에서 분리한 엑소좀은 NTA 분석을 통해 50~200 nM 크기를 보였고 ㎖당 나노입자수는 2.29×1015가 존재하는 것을 확인하였다. TEM(transmission electron microscopy)을 사용하여 관찰한 결과 이중지질막을 가지는 구형의 엑소좀을 관찰할 수 있었다(도 2).The exosomes isolated from the deer antler cells cell line showed a size of 50-200 nM by NTA analysis and the number of nanoparticles per ml was 2.29 × 10 15 . Observation using transmission electron microscopy (TEM) revealed a spherical exosome with a bilayer lipid membrane (FIG. 2).
피부개선 효과Skin improvement effect
3.1 피부 세포 증식3.1 Skin cell proliferation
ATCC(American Type Culture Collection)에서 구입한 인간지방유래-중간엽줄기세포(Adipose-derived Stem Cell, ASC), 인간피부표피세포(HaCat), 인체진피섬유아세포(HDF)를 DMEM/F12 nutrient 배지만을 사용하여 배양하였고, 본 발명에 따른 조성물(녹용 유래 줄기세포 배양액)의 상기 세포(ASC, HaCat 및 HDF) 증식에 대한 효과를 확인하였다. 인간피부표피세포(HaCat) 및 인간 진피섬유아세포(HDF)의 증식률 증가는 피부의 항-노화 또는 항-주름 효과를 검증하는 결과이다. 각 세포에 음성대조군(NC, Negative Control, PBS, Phosphate-Buffered Saline), 녹용유래 줄기세포 배양액(DC), 녹용추출물 10㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DE10), 녹용추출물 100㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DE100), 및 인간지방유래중간엽줄기세포 배양액(AAPE)을 각각 100% 처리한 후, 각 세포의 증식률을 각각 비교하였다. 음성대조군에 비해 각 처리군에서 세포의 증식이 모두 증가하였다. 특히, AAPE보다 녹용줄기세포 배양액 처리시 세포의 증식이 더 증가하였는데, HaCat 및 HDF 세포에서 DE100군 처리시 대조군에 비해 40 내지 70%의 증식률 증가를 확인하였다(도 3). Adipose-derived stem cells (ASC), human skin epidermal cells (HaCat) and human dermal fibroblasts (HDF) purchased from the American Type Culture Collection (ATCC) were inoculated into DMEM / F12 nutrient medium , And the effect of the composition according to the present invention (the antler stem cell culture solution) on the proliferation of the cells (ASC, HaCat and HDF) was confirmed. Increasing the proliferation rate of human skin epidermal cells (HaCat) and human dermal fibroblast (HDF) is the result of verifying the anti-aging or anti-wrinkle effect of the skin. (DE10), a deer antler extract (100), which was cultured in each cell containing a negative control (NC, Negative Control, PBS, Phosphate-Buffered Saline), a deer antler stem cell culture (DE100) and human adipose derived mesenchymal stem cell culture medium (AAPE), which were cultured in the presence of 100 ㎍ / ㎖ of each of the cells, were compared with each other. Cell proliferation was increased in each treatment group compared to the negative control group. In particular, the proliferation of stem cell cultures of deer antler was more increased than that of AAPE. In the case of treatment with DE100 group in HaCat and HDF cells, an increase in the proliferation rate of 40 to 70% was observed compared to the control (Fig. 3).
3.2 상처 치유(wound healing)3.2 wound healing
인체진피섬유아세포(HDF)를 실시예 3.1에 기재된 배양 조건과 동일한 조건으로 배양하였고, HDF에 대한 본 발명에 따른 조성물(녹용 유래 줄기세포 배양액)의 상처 치유 효과를 측정하였다. 배양 중인 HDF에 스크래치(scratch)를 낸 후, 음성대조군(NC), 녹용유래 줄기세포 배양액(DC), 녹용추출물 10㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DE10), 녹용추출물 100㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DE100), 및 인간지방유래줄기세포 배양액(AAPE)을 처리하였다. 상처 치유 효과를 비교한 결과, 24시간 후에는 DE100군에서 가장 높은 회복률을 보였는데, 48시간 또는 96시간 후에는 DE100군과 AAPE군에서 유사한 회복률을 확인하였다(도 4).Human dermal fibroblast (HDF) was cultured under the same culture conditions as described in Example 3.1, and the wound healing effect of the composition according to the present invention (HDF-derived stem cell culture solution derived from antler) was measured. After scratching the HDF in culture, the antler stem cell culture solution (DE10) cultured in the negative control (NC), the antler stem cell culture solution (DC) and the antler extract 10 .mu.g / / Ml, and a human adipose-derived stem cell culture (AAPE). Comparing wound healing effects, the highest recovery rate was observed in the DE100 group after 24 hours, and similar recovery rates were observed in the DE100 group and the AAPE group after 48 hours or 96 hours (FIG. 4).
탈모 개선Hair loss improvement
4.1 모유두세포의 증식4.1 Propagation of dermal papilla cells
ATCC(American Type Culture Collection)에서 구입한 모유두세포는 모낭 형성 및 모발 형성에 가장 중요한 세포이다. 모유두세포를 각 well에 8000~10000 cell씩 DMEM/F12 nutrient 배지만을 사용하여 배양한 후, 음성대조군(NC), 녹용유래 줄기세포 배양액(DC), 녹용추출물 10㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DC+DE10), 녹용추출물 100㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DC+DE100), 및 인간지방유래줄기세포 배양액(AAPE)을 처리하여 3일간 배양하였다. 모유두세포의 증식률을 관찰한 결과, 음성대조군에 비해 DC, DC+DE10군, 및 AAPE군은 25~50%, DC+DE100군은 95%의 증식률 증가를 확인하였다(도 5).The dermal papilla cells purchased from the American Type Culture Collection (ATCC) are the most important cells for hair follicle formation and hair formation. The dermal papilla cells were cultured in DMEM / F12 nutrient medium at 8000 to 10000 cells per well, and then cultured in a negative control (NC), a deer stem cell culture solution (DC), and 10 μg / (DC + DE100) and human adipose stem cell culture (AAPE), which were cultured in a medium containing 100 μg / ml of a stem cell culture solution (DC + DE10) and a deer antler, were cultured for 3 days. As a result of observing the proliferation rate of the dermal papilla cells, the proliferation rate of the DC, DC + DE10, and AAPE groups was increased by 25 to 50% and 95% by the DC + DE100 group, respectively (FIG. 5).
추가적으로, 녹용 줄기세포 배양액으로부터 분리한 엑소좀의 모유두세포 증식에 대한 효과를 시험하였다. 모유두세포를 각 well에 8000~10000 cell씩 DMEM/F12 nutrient 배지만을 사용하여 배양하였다. 이후, 세포를 배양하지 않은 상태의 배양액을 처리한 음성대조군(Control) 또는 녹용추출물 10㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DC+DE10)에서 추출한 엑소좀(DAMSC-CM EVs)을 처리하여 모유두세포를 3일간 배양하였다. 각 처리군에서, Wnt 신호전달 경로에 관련된 유전자로서 모낭 형성시 발현이 증가하는 3가지 유전자 Wnt-3a, Wnt-10 b 및 LET-1 의 발현 수준을 실시간 PCR(real time PCR)에 의해 비교하였다. 엑소좀 처리군은 3가지 유전자 Wnt-3a, Wnt-10 b 및 LET-1의 발현이 모두 증가하였다(도 6).In addition, the effect of exosomes isolated from the antler stem cell culture on the development of dermal papilla cells was examined. Dermal papilla cells were cultured in DMEM / F12 nutrient medium at 8000 ~ 10000 cells per well. Thereafter, the exosomes (DAMSC-CM EVs) extracted from the antler stem cell culture solution (DC + DE10) cultured in the negative control (Control) or 10 μg / ml of the antler extract And the dermal papilla cells were cultured for 3 days. The expression levels of three genes, Wnt-3a, Wnt-10b and LET-1, which are expressed in the Wnt signaling pathway during hair follicle development, were compared by real time PCR . The expression of the three genes Wnt-3a, Wnt-10b and LET-1 increased in the exosomal treated group (Fig. 6).
4.2 신호전달 경로4.2 Signaling Paths
모유두세포의 증식에 관련된 신호전달 경로인 Wnt 신호전달 경로의 활성화를 시험하였다. 모유두세포에 음성대조군(NC), 녹용유래 줄기세포 배양액(DC), 녹용추출물 10ug/ml을 포함하여 배양한 녹용줄기세포 배양액(DC+DE10), 녹용추출물 100 ㎍/㎖을 포함하여 배양한 녹용줄기세포 배양액(DC+DE100), 및 인간지방유래줄기세포 배양액(AAPE)를 처리하여 배양하였다. 각 처리군에서, Wnt 신호전달 경로에 관련된 유전자로서 모낭 형성시 발현이 증가하는 2가지 유전자 Wnt-3a 및 Wnt-10 b의 발현 수준을 실시간 PCR(real time PCR)에 의해 비교하였다. AAPE 처리군에서 하나의 유전자 Wnt-3a의 발현이 증가하였으나, DC+DE100 처리군은 2가지 유전자 Wnt-3a 및 Wnt-10 b의 발현이 모두 증가하였다(도 7).Activation of the Wnt signaling pathway, a signaling pathway involved in the proliferation of dermal papilla cells, was examined. The dermal papilla cells were cultured in a solution containing 10 μg / ml of negative control (NC), deer antler stem cell culture (DC), 10 μg / ml of antler extract and 100 μg / ml of deer antler Stem cell culture medium (DC + DE100), and human adipose stem cell culture medium (AAPE). In each treatment group, the expression levels of Wnt-3a and Wnt-10b, two genes whose expression during hair follicle development increased as a gene related to the Wnt signaling pathway, were compared by real time PCR. In the AAPE-treated group, the expression of one gene of Wnt-3a was increased, but the expression of Wnt-3a and Wnt-10b of the two genes was increased in DC + DE100 treated group (FIG. 7).
줄기세포의 성장인자 분비Growth factor secretion in stem cells
본 발명에 따른 녹용줄기세포 배양액의 인체 내 중간엽 줄기세포에 대한 paracrine 분비의 증진 효과를 시험하였다. 상기 ASC(인간지방유래중간엽 줄기세포)에 음성대조군(NC), 배양액 DC+DE100, 배양액 AAPE를 각각 100%로 처리한 후 2일간 배양 후, 무혈청 배지로 교환하여 3일간 배양하였다. 무혈청 배양 3일 후에, 배양액 내 성장 인자(growth factor)의 수준을 단백질 칩(protein chip)을 통해 비교하였다. 음성대조군 및 AAPE군에 비해, DC+DE100군에서 성장인자(EGF, IGF, TGF, VEGF 등)의 분비가 증가하였음을 확인하였다(도 8). 이러한 결과는 본 발명에 따른 녹용줄기세포 배양액이 체내 중간엽 줄기세포로부터 성장인자의 측(paracrine) 분비를 증가시킬 수 있는 가능성을 갖추고 있음을 제시하는 결과이다.The enhancing effect of paracrine secretion on the mesenchymal stem cells in the human cornea of the deer antler according to the present invention was tested. The ASCs (human adipose-derived mesenchymal stem cells) were treated with negative control (NC), medium DC + DE100, and culture AAPE at 100%, respectively, for 2 days and then changed to serum-free medium for 3 days. Three days after serum-free culture, the levels of growth factors in the culture medium were compared via protein chips. (EGF, IGF, TGF, VEGF, etc.) were increased in the DC + DE100 group as compared to the negative control group and the AAPE group (FIG. 8). These results indicate that the antler stem cell culture solution according to the present invention has the possibility of increasing the secretion of growth factors from the mesenchymal stem cells in the body.
Claims (22)
A composition for preventing or treating hair loss or promoting hair growth or hair growth comprising an antler stem cell culture liquid as an effective ingredient.
상기 녹용 줄기세포 배양액은 녹용 추출물을 포함하는 배지에서 녹용 줄기세포를 배양하여 제조된 것인, 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물.
The method according to claim 1,
The composition for preventing or treating hair loss, or for promoting hair growth or hair growth, wherein the deer antler stem cell culture liquid is prepared by culturing a deer stem cell in a medium containing a deer antler.
상기 녹용 추출물은 5 내지 200 ㎍/㎖인, 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물.
3. The method of claim 2,
The composition for preventing or treating hair loss or promoting hair growth or hair growth, wherein the antler extract has a concentration of 5 to 200 占 퐂 / ml.
상기 조성물은 모낭형성을 촉진시키는 것을 특징으로 하는, 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물.The method according to claim 1,
Wherein said composition promotes hair follicle formation. 2. The composition for promoting hair loss or hair growth according to claim 1, wherein said composition promotes hair follicle formation.
상기 조성물은 Wnt 신호전달 경로 내 유전자의 발현을 증가시키는 것을 특징으로 하는, 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물.
The method according to claim 1,
Wherein the composition increases the expression of a gene in the Wnt signaling pathway, or for promoting hair growth or hair growth.
상기 유전자는 Wnt -3a 또는 Wnt -10b인 것을 특징으로 하는, 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물.
6. The method of claim 5,
Wherein the gene is Wnt- 3a or Wnt- 10b .
A composition for preventing or treating hair loss or promoting hair growth or hair growth comprising exosome derived from an antler stem cell culture liquid as an active ingredient.
상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용 유래 줄기세포를 배양하여 제조된 것을 특징으로 하는, 탈모 예방 또는 치료용, 또는 발모 또는 육모 촉진용 조성물.
8. The method of claim 7,
The composition for preventing or treating hair loss, or for promoting hair growth or hair growth, wherein the deer antler cell culture solution is prepared by culturing a stem cell derived from antler in a medium containing 5 to 200 占 퐂 / ml of antler extract.
A pharmaceutical composition for the treatment of wounds comprising an antler stem cell culture fluid as an active ingredient.
상기 상처는 피부에 있는 것으로, 베임(cut), 절개(incisions), 찰과상(abrasions), 열상 또는 찢김(lacerations), 골절(fractions), 타박(contusions), 화상(burns), 또는 절단(amputations)으로 구성된 군으로부터 선택된 어느 하나에 의한 상처인 것을 특징으로 하는, 상처 치료용 약학적 조성물.
10. The method of claim 9,
The wound is on the skin and can be cut, incisions, abrasions, lacerations, fractions, contusions, burns, or amputations. ≪ / RTI > or a pharmaceutically acceptable salt thereof.
상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용유래 줄기세포를 배양하여 제조된 것을 특징으로 하는, 상처 치료용 약학적 조성물.
10. The method of claim 9,
The pharmaceutical composition for wound healing is prepared by culturing the antler stem cell culture solution in a medium containing 5 to 200 占 퐂 / ml of antler extract.
상기 조성물은 중간엽 줄기세포로부터 EGF, IGF, TGF, 및 VEGF로 구성된 군으로부터 선택된 어느 하나의 성장인자 분비를 촉진시키는 것을 특징으로 하는, 상처 치료용 약학적 조성물.
10. The method of claim 9,
Wherein said composition promotes secretion of any one growth factor selected from the group consisting of EGF, IGF, TGF, and VEGF from mesenchymal stem cells.
A method of treating a wound of an individual comprising administering to the subject a composition of any one of claims 9 to 12, wherein said individual is a non-human mammal.
A cosmetic composition for preventing or ameliorating skin wrinkles or skin aging comprising an antler stem cell culture liquid as an active ingredient.
상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용 유래 줄기세포를 배양하여 제조된 것을 특징으로 하는, 화장료 조성물.
15. The method of claim 14,
Wherein the deer antler cell culture solution is prepared by culturing the antler-derived stem cells in a medium containing 5 to 200 占 퐂 / ml of a deer antler extract.
상기 조성물은 피부세포의 증식을 증가시키는 것을 특징으로 하는, 화장료 조성물.
15. The method of claim 14,
Wherein said composition increases skin cell proliferation.
A composition for improving hair loss or promoting hair growth or hair growth comprising exosome derived from an antler stem cell culture liquid as an active ingredient.
상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용 유래 줄기세포를 배양하여 제조된 것인, 식품 조성물.
18. The method of claim 17,
Wherein the deer antler cells culture solution is prepared by culturing antler-derived stem cells in a medium containing 5 to 200 占 퐂 / ml of antler extract.
A composition for improving hair loss or promoting hair growth or hair growth comprising an antler stem cell culture liquid as an active ingredient.
상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용 유래 줄기세포를 배양하여 제조된 것을 특징으로 하는, 탈모 개선용, 또는 발모 또는 육모 촉진용 식품 조성물.20. The method of claim 19,
The composition for enhancing hair loss or promoting hair growth or hair growth according to any one of claims 1 to 5, wherein the deer antler cell culture solution is prepared by culturing the antler stem cells in a medium containing 5 to 200 占 퐂 / ml of antler extract.
A composition for improving skin wrinkles or skin aging comprising an antler stem cell culture liquid as an active ingredient.
상기 녹용 줄기세포 배양액은 녹용 추출물 5 내지 200 ㎍/㎖을 포함하는 배지에서 녹용유래 줄기세포를 배양하여 제조된, 식품 조성물.
22. The method of claim 21,
Wherein the deer antler cell culture solution is prepared by culturing the antler-derived stem cells in a medium containing 5 to 200 占 퐂 / ml of antler extract.
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