KR20180042422A - 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스 - Google Patents

단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스 Download PDF

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KR20180042422A
KR20180042422A KR1020187008870A KR20187008870A KR20180042422A KR 20180042422 A KR20180042422 A KR 20180042422A KR 1020187008870 A KR1020187008870 A KR 1020187008870A KR 20187008870 A KR20187008870 A KR 20187008870A KR 20180042422 A KR20180042422 A KR 20180042422A
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cell
detection region
signal
impedance
electrical signal
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Korean (ko)
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제프리 잔
밍데 젱
데이비드 아이. 슈라이버
하오 린
제리 더블유. 샨
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룻거스, 더 스테이트 유니버시티 오브 뉴저지
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Priority to KR1020247030391A priority Critical patent/KR20240144409A/ko
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/1056—
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology

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  • Wood Science & Technology (AREA)
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  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
KR1020187008870A 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스 Ceased KR20180042422A (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020247030391A KR20240144409A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562214665P 2015-09-04 2015-09-04
US62/214,665 2015-09-04
PCT/US2016/050201 WO2017040995A1 (en) 2015-09-04 2016-09-02 High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells

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KR1020247030391A Division KR20240144409A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스

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KR20180042422A true KR20180042422A (ko) 2018-04-25

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KR1020187008870A Ceased KR20180042422A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스
KR1020247030391A Pending KR20240144409A (ko) 2015-09-04 2016-09-02 단일 세포로의 효율적인 분자 전달을 위한 고효율, 피드백-제어된 전기천공 마이크로디바이스

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US (2) US10927333B2 (enrdf_load_stackoverflow)
EP (1) EP3344746B1 (enrdf_load_stackoverflow)
JP (1) JP6989133B2 (enrdf_load_stackoverflow)
KR (2) KR20180042422A (enrdf_load_stackoverflow)
CN (1) CN109153956B (enrdf_load_stackoverflow)
AU (1) AU2016315883B2 (enrdf_load_stackoverflow)
BR (1) BR112018004278B1 (enrdf_load_stackoverflow)
CA (1) CA2997435C (enrdf_load_stackoverflow)
WO (1) WO2017040995A1 (enrdf_load_stackoverflow)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12227731B2 (en) 2017-10-19 2025-02-18 Etta Biotech Co., Ltd. Flow electroporation device

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3440186A4 (en) 2016-04-04 2019-12-11 Cytequest, Inc. SYSTEM, APPARATUS AND METHOD FOR THE ELECTROPORATION OF CELLS
WO2019125333A2 (en) * 2017-08-23 2019-06-27 Istanbul Teknik Universitesi Microfluidic system for cancer cell separation, capturing and drug screening assays
US11485944B2 (en) 2018-04-04 2022-11-01 Hewlett-Packard Development Company, L.P. Microfluidic cellular membrane modification devices
US20190338235A1 (en) * 2018-05-01 2019-11-07 The Charles Stark Draper Laboratory, Inc. Method and Device for Exosomes Electroporation
CA3106252A1 (en) * 2018-07-12 2020-01-16 Gpb Scientific, Inc. Microfluidic methods for the preparation of cells
WO2020041480A1 (en) * 2018-08-21 2020-02-27 The Regents Of The University Of California High-throughput system and method for the temporary permeabilization of cells using lipid bilayers
SG11202102008XA (en) * 2018-08-31 2021-03-30 Charles Stark Draper Laboratory Inc Method and apparatus for high throughput high efficiency transfection of cells
CA3117715A1 (en) * 2018-10-26 2020-04-30 Kytopen Corporation Devices, systems, and kits for electroporation and methods of use thereof
CN112840015A (zh) * 2018-11-12 2021-05-25 飞秒生物医学有限公司 控制细胞内递送物质的方法及装置
JP7260933B2 (ja) * 2019-02-04 2023-04-19 ラトガーズ、ザ ステイト ユニバーシティ オブ ニュージャージー 微小電極を使用する組織電気泳動転写用デバイス
CA3138353A1 (en) * 2019-05-16 2020-11-19 CyteQuest, Inc. Devices, methods, and systems for electroporation
CN114340777A (zh) 2019-07-02 2022-04-12 凯拓盘公司 用于机电递送的装置、系统和套件及其使用方法
WO2021015779A1 (en) 2019-07-25 2021-01-28 Hewlett-Packard Development Company, L.P. Cell poration and transfection apparatuses
CN110835603A (zh) * 2019-10-09 2020-02-25 遵义医科大学珠海校区 一种快速实现细胞膜可逆性损伤的装置及方法
EP3871773A1 (en) * 2020-02-27 2021-09-01 Cellix Limited A method of determining the transfection status of a plurality of cells
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
US20220071692A1 (en) * 2020-09-08 2022-03-10 Biosense Webster (Israel) Ltd. Impedance based irreversible-electroporation (ire)
US20220126292A1 (en) * 2020-10-28 2022-04-28 Northwestern University Microfluidic device for live cell manipulation and analysis
US12350682B2 (en) * 2021-03-19 2025-07-08 National Yang Ming Chiao Tung University Microelectrode device, microfluidic chip, and microfluidic examination method
EP4599040A2 (en) * 2022-10-07 2025-08-13 Rutgers, the State University of New Jersey High throughput, feedback-controlled electroporation microdevice for efficient molecular delivery into single cells
WO2024259237A2 (en) * 2023-06-15 2024-12-19 Georgia Tech Research Corporation Targeted feedback-controlled high efficiency single cell electroporation for gene and cell therapy
WO2025128607A1 (en) * 2023-12-11 2025-06-19 Triple Ring Technologies, Inc. Method and apparatus for high-throughput transfection of cells using electroporation

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07184686A (ja) * 1993-12-28 1995-07-25 Nec Corp 細胞活性測定方法
US7456012B2 (en) 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
US7053063B2 (en) * 1999-07-21 2006-05-30 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes in tissue
US6403348B1 (en) * 1999-07-21 2002-06-11 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
IT1320187B1 (it) * 2000-04-21 2003-11-26 Igea Srl Dispositivo e metodo di elettro-porazione che fornisce un segnalemodulato sotto il controllo continuo della elettro-permeabilizzazione
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
US20050170510A1 (en) * 2003-12-08 2005-08-04 Yong Huang Device and method for controlled electroporation and molecular delivery into cells and tissue
US8058056B2 (en) * 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
WO2006112870A1 (en) * 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US7951582B2 (en) * 2005-12-19 2011-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Systems and methods for analyzing and manipulating biological samples
ITTO20060278A1 (it) 2006-04-13 2007-10-14 Silicon Biosystem S P A Metodo per la selezione e/o il processamento di particelle, in particolare cellule
US7678256B2 (en) * 2006-11-03 2010-03-16 Sandia Corporation Insulator-based DEP with impedance measurements for analyte detection
CN101616839B (zh) * 2007-02-08 2013-03-13 维姆工程集团有限公司 船用螺旋桨螺距调节装置
CN102680526B (zh) * 2012-05-16 2014-07-02 清华大学 单细胞阵列微芯片及其制造、电测量和电穿孔方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12227731B2 (en) 2017-10-19 2025-02-18 Etta Biotech Co., Ltd. Flow electroporation device

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KR20240144409A (ko) 2024-10-02
US20180258379A1 (en) 2018-09-13
AU2016315883A1 (en) 2018-03-29
WO2017040995A1 (en) 2017-03-09
CN109153956B (zh) 2022-10-28
EP3344746B1 (en) 2021-07-21
US10927333B2 (en) 2021-02-23
JP6989133B2 (ja) 2022-01-05
CA2997435A1 (en) 2017-03-09
EP3344746A1 (en) 2018-07-11
BR112018004278B1 (pt) 2022-05-03
CN109153956A (zh) 2019-01-04
JP2018529333A (ja) 2018-10-11
BR112018004278A2 (enrdf_load_stackoverflow) 2018-10-02
US11987777B2 (en) 2024-05-21
EP3344746A4 (en) 2019-01-16
CA2997435C (en) 2022-11-08
AU2016315883B2 (en) 2021-01-28
US20210139825A1 (en) 2021-05-13

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St.27 status event code: U-3-3-T10-T11-oth-X000

T13-X000 Administrative time limit extension granted

St.27 status event code: U-3-3-T10-T13-oth-X000

PA0104 Divisional application for international application

St.27 status event code: A-0-1-A10-A18-div-PA0104

St.27 status event code: A-0-1-A10-A16-div-PA0104