KR20180041107A - Composition for improving skin - Google Patents
Composition for improving skin Download PDFInfo
- Publication number
- KR20180041107A KR20180041107A KR1020180043915A KR20180043915A KR20180041107A KR 20180041107 A KR20180041107 A KR 20180041107A KR 1020180043915 A KR1020180043915 A KR 1020180043915A KR 20180043915 A KR20180043915 A KR 20180043915A KR 20180041107 A KR20180041107 A KR 20180041107A
- Authority
- KR
- South Korea
- Prior art keywords
- skin
- composition
- extract
- stem cells
- present
- Prior art date
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Abstract
Description
본 발명은 피부 재생, 아토피 개선, 피부 보습, 피부결 개선, 줄기세포 활성 촉진 효과를 나타내는 피부 개선용 조성물에 관한 것이다. The present invention relates to a skin improving composition showing skin regeneration, atopic improvement, skin moisturizing, skin texture improvement, and stem cell activity promoting effect.
피부의 최외각에 위치하고 있는 표피(epidermis)는 외부의 다양한 물리적, 화학적 및 기계적 자극에 대한 방어와 피부를 통한 체내 수분의 과도한 발산을 막는 보호 기능을 수행하고 있다. 이러한 보호 기능은 각질형성세포로 구성된 각질층이 정상적으로 형성되고 유지됨으로써 가능하다. 각질형성세포는 표피최하층(stratum basale)에서 지속적으로 증식하던 기저세포(basal cell)가 각질층(stratum corneum)으로 이동하면서, 단계적으로 형태 및 기능상의 변화를 거치며 형성된 세포이며, 일정 기간이 지나면 오래된 각질형성세포는 피부에서 탈락되고, 표피 최하층으로부터 올라온 새로운 각질형성세포가 그 기능을 대신하는 표피 분화(epidermis differentiation) 또는 각화(keratinization)의 과정을 반복하게 된다. 이러한 각화과정에서 각질형성세포는 천연보습인자(Natural Moisturizing Factor; NMF)와 세라마이드, 콜레스테롤 및 지방산과 같은 세포 간 지질을 생성하여, 각질층이 외부와의 차단층 역할을 하게 됨으로써 피부장벽(skin barrier)로서의 기능을 보유하게 된다.The epidermis, located at the outermost part of the skin, has a protective function to protect against various external physical, chemical and mechanical stimuli and to prevent excessive divergence of body water through the skin. This protective function is possible by normally forming and maintaining the stratum corneum composed of keratinocytes. The keratinocyte is a cell formed by a stepwise change in morphology and function while a basal cell that continuously proliferates in the stratum basale moves to the stratum corneum, Forming cells are removed from the skin and the new keratinocytes from the epidermis's bottom layer repeat the process of epidermis differentiation or keratinization replacing its function. In this keratinization process, keratinocytes produce intercellular lipids such as natural moisturizing factors (NMF) and ceramides, cholesterol, and fatty acids, which act as barrier layers to the outside, As shown in Fig.
또한, 현대 사회의 주요 질환의 하나로 여겨지는 피부 건조증은 피부 장벽 기능 이상에 의해 야기되는 증상의 하나로, 최근, 환경오염, 아파트, 고층 건물과 같은 건조한 환경의 증가, 사회적 스트레스의 증가, 우리나라 특유의 과도한 목욕 문화, 피부 노화 등과 같은 여러 가지 요인에 의해 점점 더 증가하고 있으며, 증세가 심하여 치료가 있어야 하는 경우 또한 지속적으로 증가하고 있다.In addition, skin dryness, which is considered to be one of the major diseases of modern society, is one of symptoms caused by skin barrier function abnormality. Recently, environmental pollution, increase in dry environment such as apartments and high rise buildings, increase in social stress, Excessive bathing culture, skin aging, and the like, and it is also increasing steadily when the condition is serious and there is a need for treatment.
최근 소아의 10%에서 발병하는 아토피성 피부염 역시 피부 건조증, 보다 근본적으로는 피부 장벽 기능의 이상을 주요 원인으로 하는 것으로 알려져 있다. 이러한 아토피성 피부염을 치료하기 위하여, 종래에는 피부 내 적절한 수분 유지에 중점을 두어 외부로부터 수분을 공급하거나 체내로부터의 수분 손실을 최소화하기 위한 연구가 많이 진행되어 왔으며, 실제로 수분 보유 능력이 있는 세라마이드(ceramide) 또는 그의 유도체와 같은 보습제가 개발되어 제약 또는 화장품 영역에서 많이 사용되고 있다.Atopic dermatitis, which is present in 10% of children in recent years, is also known to be a cause of dry skin syndrome, and more fundamentally, abnormal skin barrier function. In order to treat such atopic dermatitis, studies have been carried out in order to supply water from the outside or minimize water loss from the body by focusing on proper moisture retention in the skin in the past. Actually, ceramide or derivatives thereof have been developed and are widely used in the pharmaceutical or cosmetic field.
그러나 이와 같은 보습제의 사용은 그 대부분이 근본적인 치료이기보다는 일시적인 증상의 완화에 불과하여, 아토피성 피부염을 포함하는 피부 건조증 및 피부 장벽 기능 이상의 치료에 대한 충분한 효과를 나타내지 못하고 있다. 이에, 손상된 피부 장벽을 근원적으로 재생시켜주는 물질의 개발이 시급한 실정이다.However, most of the use of such moisturizers is merely a temporary symptom relief rather than a fundamental treatment, and thus does not show sufficient effect for treatment of dry skin and skin barrier function including atopic dermatitis. Therefore, it is urgent to develop a substance that can restore the damaged skin barrier fundamentally.
최근 들어, 각질 형성 세포에 존재하는 PPAR 알파(Peroxisome Proliferator Activated Receptor-alpha)가 그 리간드(ligand)와 결합하여 활성화되면, 각질 형성 세포의 분화를 촉진시켜 손상된 피부 장벽을 재구성하는 효과가 있다고 알려졌다. 실제로 공지된 PPAR 알파의 작용제(agonist)인 클로파이브레이트(Clofibrate)[비특허문헌 1] 또는 WY14643[비특허문헌 2] 등을 피부 장벽이 손상된 피부에 도포하는 경우, 각질 형성 세포의 분화가 촉진되고 피부 장벽의 회복이 촉진됨이 확인된 바 있다.Recently, it has been known that PPAR alpha (peroxisome proliferator activated receptor-alpha) present in keratinocyte cells is activated by binding to its ligand, thereby promoting the differentiation of keratinocytes and reconstructing damaged skin barrier. In the case of applying Clofibrate (Non-Patent Document 1) or WY 14643 (Non-Patent Document 2) which is an agonist of PPAR alpha actually known to the skin with damaged skin barrier, differentiation of keratinocytes is accelerated And recovery of the skin barrier is promoted.
또한, 피부 표면의 형태인 피부결은 미세한 선에 의해 형성된 3차원 미세구조를 특징으로 하며 연령별·부위별로 유의하게 다르다. 피부결은 선의 깊이에 따라 1차(약 20-100 ㎛), 2차(약 5-40 ㎛), 3차(약 0.5 ㎛) 라인으로 구분되며, 좋은 피부일수록 각 라인들이 그물형태의 네트워크구조를 이루며 선들이 만나서 생기는 다각형과 별 모양(star formation)이 뚜렷하다는 특징을 지니고 있다[비특허문헌 3].In addition, the skin texture, which is the shape of the skin surface, is characterized by a three-dimensional microstructure formed by fine lines and is significantly different according to age and site. The skin texture is divided into primary (about 20-100 ㎛), secondary (about 5-40 ㎛), and tertiary (about 0.5 ㎛) lines depending on the depth of the line. And has a distinctive polygonal shape and star formation caused by the lines meeting [Non-Patent Document 3].
그러나 나이가 들어감에 따라 또는 피부가 손상됨에 따라 촘촘하던 미세라인의 네트워크 구조가 무너져 미세 라인(2차, 3차 등)은 사라지고 1차 라인은 더욱 깊어져 주름이 생성되는 것으로 알려져 있다[비특허문헌 4,5]. 즉, 피부가 노화됨에 따라 나타나는 대표적인 징후인 주름은 이미 장기간에 걸쳐 일어나는 미세한 변화의 축적이다.However, as the age increases or the skin becomes damaged, the network structure of the dense fine lines collapses and fine lines (secondary, tertiary, etc.) disappear and the primary lines become deeper and wrinkles are known to be produced Literature 4,5]. In other words, wrinkles, a typical sign of skin aging, are accumulations of minute changes that have already occurred over a long period of time.
발생학적으로 사람 피부의 모든 구성성분은 외배엽이나 중배엽에서 유래되는 것으로 알려져 있다. 표피, 모낭, 피지선 및 땀샘선 등은 외배엽에서 기원하며, 멜라닌 세포, 신경 및 특수 감각 수용체는 신경외배엽에서 유래된다. 발생학적 시기에 따라 배아의 줄기세포는 분화를 거듭하여 각각의 조직 기능에 맞는 특성의 세포가 되고, 성체가 된 후에도 일정수의 줄기세포는 조직에 남게 되는데 피부에서는 주로 두 곳에 줄기세포들이 존재한다. 첫째는 모낭(hair follicle)에 존재한다. 이곳은 세포분화가 일어나기 전의 세포로 표피의 재생과 관련해 중요한 역할을 담당하는 것으로 알려져 있으며, 털의 재생과 성장에도 중요한 역할을 수행하고 있다. 둘째는 표피의 기저층(the basal layer of epidermis)이다. 이곳에 자리하고 있는 줄기세포는 표피는 물론 진피층의 섬유아세포까지 관장하여 피부 건강을 지키는 중요한 역할을 수행하고 있음이 밝혀졌다. 이곳의 줄기세포는 상대적으로 양이 많고 쉽게 얻을 수 있다는 장점이 있어 피부줄기세포 연구에 널리 사용되고 있다. 피부는 지속적으로 재생(renew)되며, 피부의 상피에 존재하는 줄기세포 즉, 피부의 상피줄기세포(epidermal stem cells)는 상처 후 상피의 복구에 관여한다[비특허문헌 6]. 상기 피부의 상피줄기세포는 피부줄기세포(skin stem cells)로도 칭해지며, 상기 피부줄기세포를 활성화시킬 경우, 외상 등의 피부상처를 치료하거나 상처 치료를 촉진할 수 있으며 피부의 주름완화 또한 기대할 수 있다. 피부줄기세포의 지표로는 인테그린β1(integrin β1) 및 인테그린 α6(integrin α6)이 사용되고 있고, 상피 형태 형성 및 분화에 필요한 피부줄기세포의 유지는 p63 단백질에 의해 조절된다고 보고되고 있다. Embryologically, all components of human skin are known to originate from ectoderm or mesodermal lobe. Epidermis, hair follicles, sebaceous glands and glands are originated from ectoderm, and melanocytes, nerves and special sensory receptors are derived from neuroepithelial cells. According to the developmental stage, the embryonic stem cells are repeatedly differentiated and become cells with the characteristics of each tissue function. After the embryo, a certain number of stem cells remain in the tissue. . The first is in the hair follicle. It is known to play an important role in the regeneration of the epidermis into cells before cell differentiation occurs, and plays an important role in hair regeneration and growth. The second is the basal layer of the epidermis. The stem cells found here play an important role in maintaining skin health by administering not only the epidermis but also the fibroblasts of the dermal layer. The stem cells here are relatively large in quantity and easy to obtain, making them widely used in the study of skin stem cells. The skin is constantly renewed, and stem cells present in the epithelium of the skin, that is, epidermal stem cells of the skin, are involved in the repair of the epithelium after injury [Non-Patent Document 6]. The epithelial stem cells of the skin are also referred to as skin stem cells. When the skin stem cells are activated, it is possible to treat skin wounds such as trauma, promote wound healing, have. Integrin β1 and integrin α6 are used as indicators of dermal stem cells, and maintenance of skin stem cells necessary for epithelial morphogenesis and differentiation has been reported to be regulated by p63 protein.
피부줄기세포는 피부의 건강과 생리학적 및 생화학적인 항상성을 유지하는데 중요한 역할을 수행한다. 피부에 존재하는 줄기세포도 노화의 영향으로 인하여 기능에 이상이 발생하게 되고, 이에 따라 피부의 항상성이 깨지면서 여러 가지 문제가 발생된다. 따라서 이러한 줄기세포의 활성을 통하여 피부노화의 여러 현상을 개선할 수 있을 것이다[비특허문헌 7].Dermal stem cells play an important role in maintaining the health and physiological and biochemical homeostasis of the skin. Stem cells present in the skin are also abnormally functioned due to the effect of aging, and thus various problems arise as the homeostasis of the skin is broken. Therefore, various phenomena of skin aging can be improved through the activation of stem cells [Non-Patent Document 7].
또한, 생체에 안전하고, 유효성분이 안정하며, 무엇보다도 기존의 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 효과가 있는 물질보다 효과가 우수한 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 활성을 지닌 성분의 개발이 절실히 요망되고 있다.In addition, it is safe to use in the living body, the active ingredient is stable, and most of all, the skin regeneration, atopic improvement, skin moisturization and skin texture improving activity which are more effective than the substances having the skin regeneration, atopic improvement, skin moisturizing, It is urgently required to develop a component having an amino acid sequence.
한편, 여지는 양귀비가 즐겨 먹었다고 전해지며, 중국 남부에서는 과일의 왕이라고도 불린다. 무환자나무목 무환자나무과에 속하며, 높이 10∼15m 정도인 상록수로서, 여지핵은 여지의 씨앗을 말한다. 영어명인 리치(litchi, lychee)라고도 한다. 여지 열매는 달걀꼴 또는 원형이며, 세로지름 3∼4㎝, 가로지름 2.5∼3㎝이다. 열매껍질은 혁질이고, 표면에는 중앙이 돌출한 딱지모양의 째진 무늬가 있다. 과육은 유백색이고 수분이 많은데, 약간 신맛과 단맛이 조화되어 맛이 좋으며, 독특한 방향이 있다. 열매는 생식하며, 아열대지방의 습윤한 산성토양에서 잘 자라고, 중국 남부 원산의 과수로서 열대지방에서 널리 재배한다.On the other hand, it is said that the poppy enjoyed it, and it is also called the king of fruits in southern China. It is an evergreen tree with a height of 10 ~ 15m, which is a seedless seed. Also known as the English name litchi (lychee). The foliar fruit is oval or round, with a diameter of 3 ~ 4㎝ and a diameter of 2.5 ~ 3㎝. The fruit skin is swollen, and the surface has a scabbled pattern with a protruding center. The flesh is milky white and has a lot of moisture. It has a slightly acidic and sweet taste that harmonizes with the taste and has a unique direction. The fruit grows and grows well in the humid acid soil of the subtropical region. It grows widely in the tropics as a fruit of the origin of southern China.
종자인 여지핵은 타원형-난원형으로 한쪽이 쭈그러져 납작하고 길이 12-22mm, 지름 6-16mm이다. 바깥면은 어두운 홍색-자흑색으로 매끈매끈하며 광택을 띄고 드문드문 세로로 쭈그러진 주름이 있으며 한쪽 끝은 둔한 원형이고 질은 비교적 단단하다. The seedless seedless nucleus is elliptical-oval, one side is flattened and flat, 12-22mm in length and 6-16mm in diameter. Outer surface is dark red to black-white, smooth, glossy, wrinkled with a sparse, wrinkled at one end, dull, and relatively hard.
여지핵은 차가운 기운을 몰아내고 통증을 완화시키며 산통, 고환염, 복통 등의 증상에 사용되어 왔다(본초강목). 여지핵에 함유된 화학성분으로는 α-(메틸렌사이클로프로필)글리신(α-(methylencyclopropyl)glycine), 3-아세토인(3-acetoin), 2,3-부탄디올(2,3-butanediol), 코팬(copaene), 시스-카리오필렌(cis-caryophyllene), 알로-아로마덴드렌(allo-aromadendrene), 휴물렌(humulene), δ-카디넨(δ-cadinene), α-커큐멘(α-curcumene), 카라메넨(calamenene), 레돌(ledol), 구아이아줄렌(guaiazulene), 잔토르히졸(xanthorrhizol), 파미틱산(palmitic acid) 등이 알려져 있다(한약약재도감).Nuclear waste has been used for symptoms such as colic, tinnitus, and abdominal pain, which relieve pain and relieve cold aura. The chemical components contained in the foliar nucleus include α- (methylenecyclopropyl) glycine, 3-acetoin, 2,3-butanediol, cobalt, copaene, cis-caryophyllene, allo-aromadendrene, humulene, delta -cadinene, alpha-curcumene, Calamenene, ledol, guaiazulene, xanthorrhizol, palmitic acid, and the like are known.
이러한 여지핵의 많은 인체 효능에도 불구하고, 여지핵의 피부와 줄기세포에 대한 연구는 전무한 실정이다.Despite the many human efficacy of this fleece nucleus, there is no research on the skin and stem cells of the fleece nucleus.
이에, 본 발명자들은 여지핵 추출물이 피부줄기세포의 증식을 촉진하여 피부의 재생을 촉진시키고, PPAR 알파의 활성도 우수하여 아토피 개선 효과가 있으며, 필라그린 발현 증가로 인한 피부 보습 개선 효과를 나타내며, 피부 밝기 개선으로 인한 피부결 개선 효과와 더불어, 피부줄기세포의 증식을 촉진하고, 줄기세포성 유지 효과를 나타내고 있어 줄기세포 활성 촉진 효과를 확인함으로써 본 발명을 완성하게 되었다.Thus, the inventors of the present invention found that the fernaceae extract promotes the proliferation of dermal stem cells, promotes regeneration of skin, has excellent activity of PPAR alpha, has an atopic improvement effect, exhibits skin moisturizing effect due to an increase in fila green expression, The present invention has been accomplished by confirming the effect of promoting stem cell activity by promoting proliferation of skin stem cells and exhibiting stem cell maintenance effect in addition to skin texture improving effect due to brightness improvement.
따라서, 본 발명의 목적은 여지핵 추출물을 유효성분으로 포함하는 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선용 조성물을 제공하는데 있다.Accordingly, an object of the present invention is to provide a composition for improving skin regeneration, atopic ecnomance, skin moisturization, and skin texture, which comprises a foliar nuclear extract as an active ingredient.
또한, 본 발명의 다른 목적은 여지핵 추출물을 유효성분으로 포함하는 줄기세포 활성 촉진용 조성물을 제공하는데 있다.Another object of the present invention is to provide a composition for stimulating stem cell activity, which comprises a foliar nuclear extract as an active ingredient.
상기 과제를 해결하기 위한 수단으로서, 본 발명은 의약, 화장료 또는 식품 제조를 위한, 여지핵 추출물을 유효성분으로 포함하는 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선용 조성물을 제공한다.As a means for solving the above problems, the present invention provides a composition for skin regeneration, atopy improvement, skin moisturizing, and skin texture improvement, which comprises a flax seed extract as an active ingredient for the manufacture of medicines, cosmetics or foods.
상기 과제를 해결하기 위한 다른 수단으로서, 본 발명은 화장료 제형 제조를 위한, 여지핵 추출물을 유효성분으로 포함하는 줄기세포 활성 촉진용 조성물을 제공한다.As another means for solving the above-mentioned problems, the present invention provides a composition for promoting stem cell activity, which comprises a flax seed extract as an active ingredient, for the production of cosmetic formulations.
본 발명에 따른 여지핵 추출물은 피부줄기세포의 증식을 촉진하여 피부의 재생을 촉진시키고, PPAR 알파의 활성도 우수하여 아토피 개선 효과가 있으며, 필라그린 발현 증가로 인한 피부 보습 개선 효과와 더불어, 피부 밝기 개선으로 인한 피부결 개선 효과를 나타냄으로써, 의약, 화장료 또는 식품 제조에 사용할 수 있다.The present invention relates to a method for the treatment and prophylaxis of skin cancer, which comprises the steps of: (a) And can be used for the manufacture of medicines, cosmetics or foods.
또한, 여지핵 추출물은 피부줄기세포의 증식을 촉진하고, 줄기세포성 유지 효과를 나타내고 있어 줄기세포 활성 촉진용 화장료 제형 제조에 사용할 수 있다. In addition, the flax seed extract promotes the proliferation of dermal stem cells and exhibits a stem cell maintenance effect, and thus can be used for the production of a cosmetic formulation for promoting stem cell activity.
이하, 본 발명의 구성을 구체적으로 설명한다.Hereinafter, the configuration of the present invention will be described in detail.
피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 성분이 실제 피부에 적용 시 우수한 효과를 발휘하기 위해서는 저농도에서 고활성의 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 활성을 나타내고, 피부를 투과하여 흡수되는 능력이 우수하고, 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 효과를 나타내기에 충분한 시간 동안 머무를 수 있도록 휘발성이 낮고, 조성물이나 피부 상에서 활성 성분이 안정하게 유지되고, 의약, 화장료 또는 식품 등으로의 제조가 용이하며, 또한 피부에 안전한 것이 바람직하다. 그러나, 공지의 성분 중 상기 특성을 모두 만족시키는 성분은 흔치 않다. 예를 들어, 몇몇 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 성분들은 시험관 내 실험 시 저농도에서도 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 활성은 우수하나, 피부를 투과하여 흡수되는 능력이 떨어져 실제 피부에 적용하기엔 어렵다. 또 다른 활성 성분들은 친수성이 낮아 의약이나 화장품, 식품으로 제형화가 어렵다. 또한, 몇몇 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 성분들은 열, 광, 또는 산소에 노출되었을 때 상기 활성 성분이 분해되거나 다른 화합물로 변형되어 피부에 적용하기 전에 이미 효과가 사라지는 경우도 있다. In order to exert an excellent effect when the skin regeneration, atopic improvement, skin moisturizing and skin texture improving effect are applied to the actual skin, it exhibits highly active skin regeneration, atopic improvement, skin moisturizing and skin texture improving activity, Is low in volatility so as to be able to stay for a sufficient time to exhibit skin regeneration, atopic improvement, skin moisturizing and skin texture improving effect and is capable of stably maintaining the active ingredient on the composition or skin, Etc., and it is preferable that it is safe for the skin. However, the components satisfying all of the above-mentioned characteristics among the known components are not common. For example, some skin regeneration, atopic improvement, skin moisturizing and skin tone improving ingredients are excellent in skin regeneration, atopic improvement, skin moisturizing and skin tone improving activity even at low concentration in in vitro test, It is difficult to apply to real skin. Other active ingredients have low hydrophilicity, making them difficult to formulate into medicines, cosmetics, and foods. In addition, some skin regeneration, atopic improvement, skin moisturizing and skin irritation improving ingredients may be degraded or transformed into other compounds when they are exposed to heat, light, or oxygen, .
하기 실시예에서 확인할 수 있는 바와 같이, 여지핵 추출물은 저농도에서 월등히 우수한 피부 줄기세포의 증식 촉진 효과, PPAR 알파 활성 효과, 필라그린 발현 효과, 피부 밝기 개선 효과를 나타내므로 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선을 위한 의약, 화장료 또는 식품 조성물의 유효성분으로 사용할 수 있다. As can be seen from the following examples, the fernaceae extract exhibits remarkably excellent proliferation promoting effect on skin stem cells, PPAR alpha activity, pilar green expression effect and skin brightness improvement at a low concentration. Therefore, skin regeneration, It may be used as an active ingredient of medicines, cosmetics or food compositions for moisturizing and improving skin texture.
따라서, 본 발명은 여지핵 추출물을 유효성분으로 포함하는 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선용 조성물을 제공한다.Accordingly, the present invention provides a composition for improving skin regeneration, atopic improvement, skin moisturization and skin texture, which comprises a fernaceae extract as an active ingredient.
상기 여지핵 추출물은 당업계에 공지된 방법에 의해 추출될 수 있으며, 그 방법은 특별히 한정되지 않는다. 또는, 시판되고 있는 추출물을 이용할 수 있다.The flax seed extract may be extracted by a method known in the art, and the method is not particularly limited. Alternatively, commercially available extracts can be used.
바람직하기로는 상기 여지핵 추출물은 여지핵을 물 및/또는 유기용매로 추출하여 수득한 추출물을 사용할 수 있으며, 상기 유기용매는 극성 유기용매, 비극성 유기용매 또는 이들의 혼합용매일 수 있다. 상기 극성 유기용매는 탄소수 1 내지 5의 저급 알코올, 에틸아세테이트 또는 아세톤일 수 있으며, 비극성 유기용매는 에테르, 클로로포름, 벤젠, 헥산 또는 디클로로메탄일 수 있다. 예를 들어, 상기 탄소수 1 내지 5의 저급 알코올은 메탄올, 에탄올, 프로판올, 부탄올 또는 이소프로판올일 수 있다. Preferably, the foliar nuclear extract may be an extract obtained by extracting a fleece nucleus with water and / or an organic solvent. The organic solvent may be a polar organic solvent, a nonpolar organic solvent or a mixture thereof. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone, and the nonpolar organic solvent may be ether, chloroform, benzene, hexane or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol or isopropanol.
한 구체예에서 상기 여지핵 추출물은 전술한 추출용매를 이용하여 추출된 1차 추출물을 극성이 다른 추출용매를 이용하여 분획한 분획물을 포함할 수 있다. 예를 들어, 여지핵 추출물은 탄소수 1 내지 5의 알코올로 추출한 후, 에테르, 벤젠, 헥산 등의 극성이 다른 용매로 다시 분획한 분획물일 수 있다. 상기 분획 시 용매는 2종 이상 사용할 수 있으며, 용매의 극성에 따라 순차적으로 사용하거나 혼합하여 사용하여, 각 용매 추출물을 제조할 수 있다. In one embodiment, the fernaceous nuclear extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent with an extraction solvent having a different polarity. For example, the fernaceous nuclear extract may be a fraction obtained by extracting with an alcohol having 1 to 5 carbon atoms and then fractionating again with a solvent having a different polarity such as ether, benzene or hexane. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent.
본 발명에서, 상기 제조된 추출물 또는 상기 분획과정을 수행하여 수득한 분획물에 대해 여과하거나 농축 또는 건조과정을 수행하여 용매를 제거할 수 있으며, 상기 여과, 농축 및 건조를 모두 수행할 수 있다. 구체적으로 상기 여과는 여과지를 이용하거나 감압여과기를 이용할 수 있고, 농축은 감압 농축기 등을 이용하여 감압 농축할 수 있으며, 건조는 동결건조법을 수행할 수 있다. In the present invention, the extracted extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and the filtration, concentration, and drying may all be performed. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a reduced pressure concentrator or the like, and the freeze-drying method can be carried out for drying.
또한, 상기 제조된 추출물 또는 상기 분획과정을 수행하여 수득한 분획물에 대해 실리카겔 컬럼 크로마토그래피(silica gel column chromatography), 박층 크로마토그래피(thin layer chromatography) 또는 고성능 액체 크로마토그래피(high performance liquid chromatography) 등의 다양한 크로마토그래피를 이용하여 정제함으로써 추가로 정제된 분획을 얻을 수 있다. The fractions obtained by performing the above-described extract or the fractionation process can be further purified by silica gel column chromatography, thin layer chromatography or high performance liquid chromatography Further purification can be achieved by purification using various chromatographic techniques.
본 발명의 조성물은 의약 제형 제조를 위해 사용할 수 있다.The compositions of the present invention may be used for the manufacture of pharmaceutical formulations.
상기 의약 제형은 오일 또는 수성 매질중의 용액, 현탁액 또는 유화액의 형태이거나, 엑스제, 분말제, 과립제, 정제 또는 캅셀제의 형태일 수 있다.The pharmaceutical formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules.
또한, 상기 조성물은 추가로 동일 또는 유사한 기능을 나타내는 유효성분을 1종 이상 함유할 수 있다. 예컨대, 공지의 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 성분을 포함할 수 있을 것이다. 추가적인 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 성분을 포함하게 되면 본 발명의 조성물의 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 효과는 더욱 증진될 수 있을 것이다. 상기 성분 추가 시에는 복합 사용에 따른 피부 안전성, 제형화의 용이성, 유효성분들의 안정성을 고려할 수 있다. 본 발명의 한 구체예에서, 상기 조성물은 당업계에 공지된 피부 재생 성분으로서, 레티노산, TGF, 동물 태반 유래의 단백질, 베튤린산 및 클로렐라 추출물, 당업계에 공지된 항산화 성분으로서, 토코페롤, 셀레늄, 비타민 C 및 페놀성 화합물, 당업계에 공지된 미백 성분으로서, 코지산(Kojic acid), 알부틴(Arbutin) 등과 같은 티로시나제 효소활성을 억제하는 물질, 하이드로퀴논(Hydroquinone), 비타민-C(L-Ascorbic acid) 및 이들의 유도체와 각종 식물 추출물로 구성되는 군으로부터 선택되는 1종 또는 2종 이상의 성분을 추가로 포함할 수 있다. 추가의 성분은 전체 조성물 중량에 대하여 0.0001 중량% 내지 10 중량%로 포함될 수 있을 것이며, 상기 함량 범위는 피부 안전성, 상기 여지핵 추출물의 제형화 시의 용이성 등의 요건에 따라 조절될 수 있을 것이다.In addition, the composition may further contain one or more active ingredients showing the same or similar functions. For example, it may contain known skin regeneration, atopic improvement, skin moisturizing and skin tone improving ingredients. The skin regeneration, atopic improvement, skin moisturizing and skin texture improving effect of the composition of the present invention may be further enhanced if the skin regeneration, atopic improvement, skin moisturization and skin texture improving ingredient are included. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the present invention, the composition is a skin regeneration component known in the art comprising retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract, antioxidant components known in the art, such as tocopherol, selenium , Vitamin C and phenolic compounds, whitening ingredients known in the art, substances inhibiting the activity of tyrosinase enzymes such as kojic acid and arbutin, hydroquinone, vitamin C (L- Ascorbic acid, derivatives thereof, and various plant extracts. The additional components may be contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition, and the content may be adjusted according to requirements such as skin safety, ease of formulation of the fernaceous nuclear extract, and the like.
또한, 본 발명의 조성물은 약학적으로 허용 가능한 담체를 더 포함할 수 있다.In addition, the composition of the present invention may further comprise a pharmaceutically acceptable carrier.
약학적으로 허용 가능한 담체는 완충액, 주사용 멸균수, 일반 식염수 또는 인산염 완충 식염수, 슈크로스, 히스티딘, 염 및 폴리솔베이트 등과 같은 여러 성분을 함유할 수 있다.Pharmaceutically acceptable carriers may contain a variety of ingredients such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates, and the like.
본 발명의 조성물은 경구 또는 비경구로 투여할 수 있으며, 일반 약학 제제의 형태, 예를 들어, 임상 투여 시 경구 및 비경구의 여러 가지 제형으로 투여될 수 있는데, 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제될 수 있다.The composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, a filler, , A binder, a wetting agent, a disintegrant, a surfactant, and the like.
경구 투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 본 발명의 의약 조성물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘 카보네이트(Calcium carbonate), 수크로스(Sucrose) 또는 락토오스(Lactose), 젤라틴 등을 섞어 조제될 수 있다.Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations can be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like can be prepared.
단순한 부형제 이외에 마그네슘 스티레이트 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순희석제인 물, 리퀴드 파라핀 이외에 여러가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다.In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of liquid formulations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used.
비경구 투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조제제, 좌제가 포함된다. 비수성용제, 현탁용제로는 프로필렌 글리콜(Propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈(tween) 61, 카카오지, 라우린지, 글리세로제라틴 등이 사용될 수 있다.Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. As a base for suppositories, witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like can be used.
본 발명에 있어서, '피부 재생 효과'라 함은 피부 외부 및 내부 원인에 의한 손상에 대하여 피부 조직이 회복되는 것을 말한다. 상기 외부 원인에 의한 손상은 자외선, 외부 오염 물질, 창상, 외상 등을 들 수 있으며, 상기 내부 원인에 의한 손상은 스트레스 등을 들 수 있다.In the present invention, the term " skin regeneration effect " refers to restoration of skin tissue against damage caused by external or internal causes of the skin. Damage due to external causes may include ultraviolet rays, external contaminants, wound, trauma, etc. The damage caused by the internal causes may be stress.
본 발명에 있어서, '아토피 개선 효과'라 함은 아토피, 피부건조증과 같은 피부질환 예방 및 치료 효과를 나타내는 것으로, 아토피 증상을 억제 또는 저해하거나, 완화시키는 것을 말한다. In the present invention, the term 'atopic improvement effect' refers to the effect of preventing and treating skin diseases such as atopy and dry skin, and is to inhibit, inhibit, or alleviate atopic symptoms.
본 발명에 있어서, '보습 효과'라 함은 피부의 수분이 감소되는 것을 저해 또는 억제하거나 피부의 수분 함유량을 증가시켜 피부 표면을 매끄럽게 하며, 윤기를 부여하는 것을 말한다.In the present invention, the term 'moisturizing effect' refers to inhibiting or reducing the reduction of moisture in the skin, or increasing the moisture content of the skin to smooth and smooth the surface of the skin.
본 발명에 있어서, '피부결 개선 효과'라 함은 노화, 스트레스 등의 영향으로 피부 표면이 거칠어지는 것을 억제 또는 저해함으로써 피부 표면을 매끄럽게 하고, 광택을 부여하며, 피부 톤을 밝게 개선하는 것을 말한다.In the present invention, the term " skin texture improving effect " refers to smoothing the skin surface, imparting gloss, and brightening the skin tone by inhibiting or inhibiting roughness of the skin surface due to aging, stress, .
본 발명에 있어서, '유효량'이라 함은 손상된 피부의 재생을 촉진하거나, 아토피와 같은 피부건조증을 개선하거나, 보습 효과나, 피부결을 개선할 수 있는 추출물의 양을 의미한다. 본 발명의 조성물이 유효량의 상기 여지핵 추출물을 포함할 때 바람직한 피부 재생 효과, 아토피 개선 효과, 보습 효과 및 피부결 개선 효과를 제공할 수 있다. 본 발명의 조성물에 포함되는 상기 여지핵 추출물의 유효량은 조성물이 제품화되는 형태, 상기 화합물이 피부에 적용되는 방법 및 피부에 머무르는 시간 등에 따라 달라질 것이다. 예컨대, 상기 조성물이 약학 제형으로 제품화되는 경우에는 일상적으로 피부에 적용하게 되는 화장품으로 제품화되는 경우에 비해 높은 농도로 상기 여지핵 추출물을 포함할 수 있을 것이다. 따라서, 일일 투여량은 상기 여지핵 추출물의 양을 기준으로 0.1 내지 100 ㎎/㎏이고, 바람직하게는 30 내지 80 ㎎/㎏이고, 더욱 바람직하게는 50 내지 60 mg/kg이며, 하루 1 ∼ 6 회 투여될 수 있다. In the present invention, the term "effective amount" means an amount of the extract capable of promoting regeneration of damaged skin, improving dry skin such as atopy, or improving moisturizing effect and skin texture. When the composition of the present invention contains an effective amount of the fernaceous nuclear extract, it is possible to provide a desirable skin regeneration effect, atopic improvement effect, moisturizing effect and skin texture improving effect. The effective amount of the flax seed extract contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, the method in which the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized in a pharmaceutical formulation, it may contain a high concentration of the flax seed extract as compared with the case where it is commercialized as cosmetics to be applied to skin on a daily basis. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, and 1 to 6 mg / kg per day based on the amount of the above- ≪ / RTI >
본 발명의 조성물은 단독으로, 또는 수술, 방사선 치료, 호르몬 치료, 화학 치료 및 생물학적 반응조절제를 사용하는 방법들과 병용하여 사용할 수 있다.The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.
본 발명의 의약 제형은 피부 외용제 제형을 포함할 수 있다. The pharmaceutical formulations of the present invention may include external preparation for skin.
상기 여지핵 추출물을 피부외용제로 사용하는 경우, 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 피부용 외용제에 통상적으로 사용되는 임의의 다른 성분과 같은 피부 과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 또한 상기 성분들은 피부 과학 분야에서 일반적으로 사용되는 양으로 도입될 수 있다. In the case of using the flax seed extract as an external preparation for skin, it is further possible to use a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, For example, water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for skin And any other ingredients used, such as those commonly used in the field of dermatology. The components can also be introduced in amounts commonly used in the field of dermatology.
상기 여지핵 추출물이 피부 외용제 제형으로 제공될 경우, 이에 제한되는 것은 아니나, 연고, 패취, 겔, 크림 또는 분무제와 같은 제형을 가질 수 있다.When the flax seed extract is provided as an external dermal preparation, it may have a formulation such as, but not limited to, ointments, patches, gels, creams or sprays.
또한, 본 발명의 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선용 조성물은 화장료 제형 제조를 위해 사용될 수 있다.Furthermore, the composition for skin regeneration, atopic improvement, skin moisturizing and skin texture improvement of the present invention can be used for the production of cosmetic formulations.
상기 화장료 제형은 일반적인 유화 제형 및 가용화 제형의 형태일 수 있다. 예컨대, 유연 화장수 또는 영양 화장수 등과 같은 화장수, 훼이셜 로션, 바디로션 등과 같은 유액, 영양 크림, 수분 크림, 아이 크림 등과 같은 크림, 에센스, 화장연고, 스프레이, 젤, 팩, 선 스크린, 메이크업 베이스, 액체 타입, 고체 타입 또는 스프레이 타입 등의 파운데이션, 파우더, 클렌징 크림, 클렌징 로션, 클렌징 오일과 같은 메이크업 제거제, 클렌징 폼, 비누, 바디 워쉬 등과 같은 세정제 등의 제형을 가질 수 있다. The cosmetic formulation may be in the form of a conventional emulsion formulation and a solubilized formulation. For example, creams, essences, cosmetic creams, sprays, gels, packs, sunscreens, make-up bases, liquids such as lotions such as lotion, facial lotion, body lotion, A powder, a cleansing lotion, a makeup removing agent such as a cleansing oil, a cleaning agent such as a cleansing foam, a soap, a body wash and the like.
또한, 상기 화장품은 여지핵 추출물에 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다.In addition, the cosmetic composition may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, As used herein means any emulsion or emulsion which is commonly used in emulsifying or emulsifying agents, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics Adjuvants commonly used in the cosmetics field, such as other ingredients.
상기 화장료 제형은 유효성분이 단기간 내에 피부에 머무르게 되는 메이크업 제거제, 세정제 등과 같은 워쉬-오프(wash-off) 타입의 화장품의 경우에는 비교적 높은 농도의 상기 여지핵 추출물을 포함할 수 있을 것이다. 반면, 유효성분이 장기간 동안 피부에 머무르게 되는 화장수, 유액, 크림, 에센스 등의 리브-온(leave-on) 타입의 화장품의 경우에는 워쉬-오프 타입의 화장품에 비해 낮은 농도의 상기 여지핵 추출물을 포함해도 무방할 것이다. 이에 제한되는 것은 아니나, 본 발명의 한 구체예에서, 상기 조성물은 상기 여지핵 추출물을 전체 조성물 중량에 대하여 0.0001 중량% 내지 10 중량%(바람직하게는 0.0001 중량% 내지 1 중량%)로 포함할 수 있다. 본 발명의 조성물이 상기 여지핵 추출물을 0.0001 중량% 미만으로 포함할 경우에는 충분한 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 효과를 기대할 수 없고, 10 중량%를 초과하여 포함할 경우에는 알러지 등 원치 않는 반응이 발생하거나 피부 안전성에 문제가 있을 수 있으므로 이를 방지하기 위한 것이다.The cosmetic formulation may contain a relatively high concentration of the flaxseed extract in the case of a wash-off type cosmetic such as a make-up remover, a detergent, etc. in which the active ingredient remains on the skin in a short period of time. On the other hand, in the case of leave-on type cosmetics such as lotion, cream, essence and the like in which the active ingredient remains on the skin for a long period of time, It may be possible. In one embodiment of the present invention, but not limited thereto, the composition may comprise from 0.0001% to 10% by weight (preferably 0.0001% to 1% by weight) of the follicular nucleus extract relative to the total composition weight have. If the composition of the present invention contains less than 0.0001% by weight of the fernaceous nuclear extract, sufficient skin regeneration, atopic improvement, skin moisturizing and skin texture improving effect can not be expected. If the composition contains more than 10% by weight, This is to prevent unwanted reactions or skin safety problems.
또한, 본 발명의 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선용 조성물은 식품 제형 제조를 위해 사용될 수 있다.Furthermore, the composition for skin regeneration, atopic improvement, skin moisturizing and skin texture improvement of the present invention can be used for manufacturing food formulations.
상기 식품 제형은 상기 여지핵 추출물을 음료, 차류, 향신료, 껌, 과자류 등의 식품 소재에 첨가하거나, 캡슐화, 분말화, 현탁액 등으로 제조한 식품을 의미한다. The food formulation refers to a food prepared by adding the above-described fernaceae extract to a food material such as a beverage, a tea, a spice, a gum, confectionery, or the like, or encapsulating, pulverizing or suspending.
상기 식품 제형은 일상적으로 섭취하는 것이 가능하기 때문에 높은 피부 재생, 아토피 개선, 피부 보습 및 피부결 개선 효과를 기대할 수 있어 매우 유용하다.Since the food preparation can be ingested routinely, it is very useful because high skin regeneration, atopic improvement, skin moisturizing and skin texture improving effect can be expected.
상기 여지핵 추출물을 식품첨가물로 사용하는 경우, 상기 여지핵 추출물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분의 혼합양은 그의 사용 목적(예방, 건강 또는 치료적 처치)에 따라 적합하게 결정될 수 있다. 일반적으로, 식품 또는 음료의 제조시에 본 발명의 조성물은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가된다. 그러나, 건강 및 위생을 목적으로 하거나 또는 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로도 사용될 수 있다.When the fernaceut extract is used as a food additive, the fernacept extract may be directly added or used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, when the food or beverage is produced. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .
상기 식품의 종류에는 특별한 제한은 없다. 상기 물질을 첨가할 수 있는 식품의 예로는 육류, 소세지, 빵, 초콜릿, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.
식품 제형이 음료인 경우, 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토스, 슈크로스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알코올이다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 mL 당 일반적으로 약 0.01 ∼ 0.04 g, 바람직하게는 약 0.02 ∼ 0.03 g이다.When the food is a beverage, various flavors or natural carbohydrates may be added as an additional ingredient such as a normal drink. The above-mentioned natural carbohydrates are sugar alcohols such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention.
상기 외에 식품 제형은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 식품 제형은 천연 과일주스, 과일주스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 혼합하여 사용할 수 있다. 이러한 첨가제의 비율은 크게 중요하진 않지만 본 발명의 조성물 100 중량부당 0.01 ~ 0.1 중량부의 범위에서 선택되는 것이 일반적이다.In addition to the above, the food formulations may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, And the like. Other food formulations may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
본 발명은 또한, 상기 여지핵 추출물을 유효성분으로 포함하는 줄기세포 활성 촉진용 조성물을 제공한다.The present invention also provides a composition for promoting stem cell activity comprising the above-mentioned fernaceae extract as an active ingredient.
본 발명에서 용어 ‘줄기세포’는 스스로 세포 분열을 할 수 있고, 매우 다양한 형태의 특이 세포 타입(specific cell type)으로 분화할 수 있는 능력을 갖는 세포이다. 이러한 줄기세포의 종류는 특별히 제한되지 않으며, 한 구체예에서, 상기 줄기세포는 피부줄기세포일 수 있다. 상기 ‘피부줄기세포’는 피부(표피, 진피 및 피하지방층)를 이루는 세포로 분화될 수 있는 줄기세포를 의미한다. 상기 피부를 이루는 세포는 표피에 존재하는 케라티노사이트(keratinocyte), 멜라노사이트(melanocyte) 및 진피에 존재하는 섬유아세포(콜라겐 및 엘라스틴의 생합성을 주로 담당)를 포함한다. The term 'stem cell' in the present invention is a cell capable of cell division by itself and capable of differentiating into a very specific type of specific cell type. The type of such stem cells is not particularly limited, and in one embodiment, the stem cells may be dermal stem cells. The term 'dermal stem cells' refers to stem cells that can be differentiated into cells constituting the skin (epidermis, dermis and subcutaneous fat layer). The cells that make up the skin include keratinocytes, melanocytes, and fibroblasts (mainly responsible for biosynthesis of collagen and elastin) present in the epidermis.
상기 피부줄기세포의 종류는 특별히 제한되지 않는다. 본 발명에서 사용되는 피부줄기세포는 그것이 어디로부터 유래한 것인지 관계없이 이용될 수 있다. 예를 들어, 피부줄기세포는 공지의 피부줄기세포 공급원, 예를 들어, 모낭 또는 표피의 기저층에서 얻을 수 있으며, 채취 대상인 동물은 포유동물일 수 있다. 한 구체예에서, 포유동물은 인간, 마우스, 랫트, 기니어 피그, 토끼, 원숭이, 돼지, 말, 소, 양, 영양, 개 또는 고양이를 포함할 수 있지만, 이에 한정되는 것은 아니다. 바람직하게 포유동물은 인간일 수 있다. 이러한, 피부줄기세포 공급원으로부터 피부줄기세포를 수득하는 방법에 대해서는 당업계에서 잘 알려져 있다.The kind of the skin stem cell is not particularly limited. The dermal stem cells used in the present invention can be used irrespective of where they originate from. For example, dermal stem cells may be obtained from a known source of dermal stem cells, e. G., From the basal layer of hair follicles or epidermis, and the animal to be harvested may be a mammal. In one embodiment, the mammal may include, but is not limited to, a human, a mouse, a rat, a guinea pig, a rabbit, a monkey, a pig, a horse, a cattle, a sheep, Preferably the mammal can be human. Such methods of obtaining dermal stem cells from dermal stem cell sources are well known in the art.
본 발명에 있어서, '줄기세포 활성 촉진 효과'라 함은 줄기세포 증식 촉진 효과, 및/또는 줄기세포의 특이적 지표 분자 및 자가 분열적 성질인 줄기세포성을 유지하는 효과를 포함하는 것을 말한다.In the present invention, the term 'promoting effect on stem cell activity' refers to an effect of promoting stem cell proliferation and / or an effect of maintaining stem cell characteristics, which is a specific indicator molecule and a self-cleaving property of stem cells.
또한, 본 발명의 조성물은 화장료 제형 제조를 위해 사용될 수 있다. 이러한 화장료 제형에 대해서는 앞서 여지핵 추출물을 포함하는 화장료 제형에 관한 설명에 기재된 내용과 동일하다. In addition, the compositions of the present invention can be used for the production of cosmetic formulations. These cosmetic formulations are the same as those described in the description of cosmetic formulations including the above-mentioned wormwood extract.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다. Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
제조예Manufacturing example 1: One: 여지핵A waste nucleus 추출물의 제조 Preparation of extract
여지핵을 세척하고, 분말화한 뒤, 10 배의 에탄올(w/v)에서 5 일간 냉침한 후 추출 원액을 여과, 농축한 엑기스를 얻었다. 여기에 정제수 및 에탄올의 혼합용액을 첨가하여 용해시킨 다음 여과하여 최종 추출물을 제조하였다.The lignin nuclei were washed and pulverized, and then cooled with 10 times of ethanol (w / v) for 5 days. The extract solution was filtered and concentrated to obtain an extract. To this was added a mixed solution of purified water and ethanol to dissolve and then filtered to prepare a final extract.
제조예Manufacturing example 2: 2: 무혈청Serum-free 배지조건에서Under medium conditions 피부줄기세포의Of dermal stem cells 배양 culture
Cellntec 사에서 구입한 인간 피부줄기세포(Human epidermal stem cell)를 96-웰 플레이트(5×103 cells/well)에, 소태아혈청(fetal bovine serum)과 유사한 BPE(Bovine pituitary extract)가 첨가된 CNT-57 배지(Cellntec 사)를 이용하여 24 시간 동안 5% CO2 및 37℃ 조건에서 배양하였다. 그 후, 배양액을 흡입관을 통하여 제거한 다음, PBS 용액(GibcoBRL 사)을 이용하여 배지 성분을 제거하고, BPE가 포함되지 않은 CNT-57 배지에 여지핵 추출물 5㎍/ml처리하여 5% CO2 및 37℃ 조건에서 72 시간 동안 배양하였다.Human epidermal stem cells purchased from Cellntec were added to a 96-well plate (5 x 10 3 cells / well) supplemented with BPE (bovine pituitary extract) similar to fetal bovine serum And cultured for 24 hours at 5% CO 2 and 37 ° C using CNT-57 medium (Cellntec). Thereafter, the culture medium was removed through a suction tube, and then the medium was removed using a PBS solution (GibcoBRL). 5 CO CO 2 and 5 쨉 g / ml of bare nucleus extract were treated with CNT-57 medium containing no BPE And cultured at 37 ° C for 72 hours.
실험예Experimental Example 1: One: CCKCCK -8 평가법을 통한 -8 Through evaluation method 피부줄기세포Dermal stem cells 증식 촉진 효과 Proliferation promoting effect
상기 제조예 2에서 배양된 피부줄기세포에 CCK-8(Cell counting kit-8) 평가를 실시하였다. CCK-8 평가는 세포 내 전자전달계 내의 탈수소효소(Dehydrogenase)가 테트라졸리움 염(Tetrazolium Salt)를 분해하여 생성하는 포르마잔(Formazan)의 흡광도를 측정하여 살아있는 세포의 밀도를 간접적으로 나타내는 분석방법이다. The skin stem cells cultured in Preparation Example 2 were evaluated for CCK-8 (Cell counting kit-8). The CCK-8 evaluation is an indirect method of measuring the density of living cells by measuring the absorbance of Formazan, a dehydrogenase in the intracellular electron transport system, which is formed by the decomposition of tetrazolium salt.
세포에 CCK-8 용액(배지부피의 1/10을 처리)을 37℃에서 2 시간 동안 처리한 후, 파장 450 nm에서의 흡광도를 측정함으로써 분광학적으로 측정하였다. 여지핵 추출물의 피부줄기세포의 증식 촉진 효과는 측정된 흡광도 값으로부터 대조군(추출물이 포함되지 않은 CNT-57 배지) 대비 하기 수학식 1에 따라 증식율(%)을 구하고, 그 값을 하기 표 1에 나타냈다.Cells were spectrophotometrically measured by treating CCK-8 solution (treated with 1/10 of the medium volume) at 37 ° C for 2 hours and then measuring the absorbance at 450 nm. From the measured absorbance values, the proliferation rate (%) of the skin extract of the fernaceae extract was determined by the following equation (1) against the control group (CNT-57 medium without extract) .
[수학식 1][Equation 1]
증식율(%) = (시료처리군의 흡광도/대조군의 흡광도) × 100Growth rate (%) = (absorbance of sample-treated group / absorbance of control group) x 100
표 1에 나타난 바와 같이, 여지핵 추출물을 처리한 배지조건에서의 피부줄기세포는 우수한 증식 촉진 효과를 나타내었다. As shown in Table 1, the skin stem cells in the media condition treated with the nuclear extract of the fleece showed an excellent proliferation promoting effect.
실험예Experimental Example 2: 2: 피부줄기세포의Of dermal stem cells 줄기세포성( Stem cell ( stemnessstemness ) 유지 효과 확인) Confirm maintenance effect
상기 제조예 2에서 배양된 피부줄기세포의 줄기세포성 유지 효과 확인을 위해 p63 발현 정도를 평가하였다. p63은 세포 내 전사인자로서 피부줄기세포의 유지 마커로 잘 알려져 있다. 세포의 RNA를 분리하여 cDNA를 합성한 다음, Taqman 염색액으로 실시간-PCR을 수행하여 p63의 발현 정도를 측정하였다. 본 실험에서 RNA의 농도는 S16 리보솜 RNA로 표준화하였다.The expression level of p63 was evaluated in order to confirm the stem cell maintenance effect of the dermal stem cells cultured in Production Example 2 above. p63 is an intracellular transcription factor and is well known as a maintenance marker for dermal stem cells. Cells were isolated and cDNA was synthesized. Then, real-PCR was performed with Taqman staining solution to measure the expression level of p63. The concentration of RNA in this experiment was normalized to S16 ribosomal RNA.
상기 실험 결과는 하기 표 2에 나타내었다. 하기 표 2에서 p63 증가 배수의 수치는 대조군(BPE 첨가한 CNT-57 배지) 대비 배수를 의미한다.The experimental results are shown in Table 2 below. In Table 2 below, the numerical value of the increase in p63 is a multiple of the control group (CNT-57 medium supplemented with BPE).
표 2에 나타난 바와 같이, 여지핵 추출물을 처리한 배지 조건은 피부줄기세포의 p63 발현을 촉진하여 줄기세포성 유지 효과가 우수하다.As shown in Table 2, the medium conditions treated with the nuclear extract of the fleece promoted the expression of p63 in the dermal stem cells, and thus the stem cell maintenance effect was excellent.
실시예Example 1: One: PPARPPAR 알파 활성화 촉진 효과 검증 Verification of Alpha Activation Promotion Effect
여지핵 추출물의 PPAR 알파 활성화 촉진 효과를 확인하기 위하여, 여지핵 추출물을 이용하여 하기와 같은 실험을 수행하였다. 마우스 유래의 근아세포(myoblast) 세포주인 C2C12 세포(ATCC CRL-1772)를 10% 우태아 혈청을 포함하는 DMEM(Dulbeco's Modified Eagle's Medium) 배지에 계대 배양하였다. 여기서 사용된 벡터로는, In order to confirm the promoting effect of PPAR alpha activation of the flax seed extract, the following experiment was performed using a fernaceae extract. C2C12 cells (ATCC CRL-1772), a mouse myoblast cell line, were subcultured in DMEM (Dulbecco's Modified Eagle's Medium) medium containing 10% fetal bovine serum. As the vector used herein,
1) pZEO 벡터 (Invitrogen)의 SV40 프로모터에 효모의 전사 인자인 GAL4-DBD(DNA Binding Domain)과 인간 PPAR 알파 LBD(Ligand Binding Domain)에서 Ser167 내지 Tyr468을 암호화하는 DNA 단편(유전자 은행 정보, NM 005036)을 포함하는 벡터; 1) A DNA fragment encoding GAL4-DBD (DNA Binding Domain), which is a transcription factor of yeast, and Ser 167 to Tyr 468 in human PPAR alpha LBD (Ligand Binding Domain), into a SV40 promoter of pZEO vector (Invitrogen) NM 005036);
2) GAL4 응답 서열을 루시퍼라이제 (Luciferase)를 발현하는 pGL3 벡터(Promega)의 다중 제한 효소 인지 부위에 삽입된 벡터, 및 2) the GAL4 response sequence is inserted into the multiple restriction enzyme recognition site of pGL3 vector (Promega) expressing luciferase, and
3) 트랜스팩션 대조군(transfection internal control)으로 β-갈락토시다아제(β-galactodisase)를 발현하는 벡터를 사용하였다.3) A vector expressing? -Galactodisase as a transfection internal control was used.
상기 배양한 세포를 4×104 cell로 24웰 플레이트에 도말한 후, 12시간 동안 배양하고, 상기 세 종류의 플라스미드 유전자를 일시적인 리포팩타민(Lipopectamine)을 이용하여 트랜스팩션(transient transfection)하였다. 8시간 후, 여지핵 추출물을 희석하여 처리하고 12시간 동안 배양한 다음, 1×PBS로 세척하고 1×리포터 용해 버퍼(reporter lysis buffer)로 세포를 용해시킨 후, 루시퍼라아제 분석 키트(Promega) 및 β-갈락토시다아제 분석 키트(Promega)를 사용하여 루시퍼라아제의 활성을 측정하였다. 즉, PPAR 알파 활성도는 '루시퍼라아제 활성/β-갈락토오스 활성'으로 측정하였다.The cultured cells were plated on a 24-well plate at 4 × 10 4 cells and cultured for 12 hours. The three types of plasmid genes were transiently transfected using lipopectamine. After 8 hours, the flax seed extract was diluted, cultured for 12 hours, washed with 1 x PBS, lysed with 1 x reporter lysis buffer, and lysed with luciferase assay kit (Promega) And [beta] -galactosidase assay kit (Promega) were used to measure the activity of luciferase. That is, the PPAR alpha activity was measured as " luciferase activity / beta -galactose activity ".
본 실험에서 양성 대조군으로는 PPAR 알파의 리간드 중 가장 강력한 것으로 알려진 Wy-14,643(Calbiochem)을 사용하였고, 음성 대조군으로는 시료를 녹일 때 사용한 DMSO 0.05%를 사용하였다.Wy-14,643 (Calbiochem), the most potent ligand of PPAR alpha, was used as a positive control in this experiment, and DMSO 0.05% was used as a negative control.
상기 실험 방법에 따라, 여지핵 추출물을 C2C12 세포에 처리하여 농도 변화에 따른 PPAR 알파의 활성을 측정하였으며, 그 결과를 하기 표 3에 나타내었다. 하기 표 3에서 PPAR 알파 활성도의 수치는 음성 대조군(DMSO 0.05%) 대비 백분율을 의미한다.According to the above experimental method, the activity of PPAR alpha was measured according to the concentration by treating the cryopreserved nuclear extract with C2C12 cells. The results are shown in Table 3 below. In Table 3 below, the value of PPAR alpha activity refers to the percentage of negative control (0.05% DMSO).
표 3에 나타난 바와 같이, 양성 대조군과 비교하여, 여지핵추출물은 매우 강력한 PPAR 알파 활성능을 나타낸다는 것을 확인할 수 있었다.As shown in Table 3, it was confirmed that, compared to the positive control group, the wilted nuclear extract showed a very strong PPAR alpha activity.
실시예Example 2: 2: 필라그린(Filaggrin)의Filaggrin's 발현 촉진 효과 Promoting effect of expression
여지핵 추출물을 5 ㎍/mL, 10 ㎍/mL가 되도록 하여 각각 인간 각질세포주 HaCaT 세포의 배양배지에 첨가하여 1일간 배양한 후, 세포의 RNA를 분리하고 cDNA를 합성한 뒤 Taqman 염색액으로 Real-time PCR을 수행하였다. RNA 농도는 S16 리보솜 RNA로 표준화시켰다.The cells were cultured for 1 day, and then the RNAs of the cells were separated, and cDNA was synthesized. Then, the Taqman stain solution was used for Real -time PCR was performed. The RNA concentration was normalized to S16 ribosomal RNA.
표 4에 나타난 바와 같이, 여지핵 추출물은 인간 각질세포주 HaCaT 세포의 필라그린 발현 촉진능이 있다.As shown in Table 4, the flax seed extract has the ability to promote pilar green expression of human keratinocyte HaCaT cells.
실시예Example 3: 3: 각질박리(turn-over)개선에To improve the turn-over of the skin 대한 About 효능성Efficacy
하기 제제예 2의 영양크림에 대해서 건강한 20대에서 50대의 여성을 대상으로 각질 박리 개선 효과를 다음과 같이 시험하였다.About the nutritional cream of the following formulation example 2, the effect of the improvement of the exfoliation of the skin in the healthy twenties to 50 women was tested as follows.
20세에서 50세까지의 여성 20명의 시험 부위에 DHA(Dihydroxyacetone, Sigma Aldrich, USA) 1.5% 용액을 8시간 동안 폐쇄첩포하여 침착시킨 후 제제예 2의 영양크림을 매일 2회 시험 부위에 도포하였다. 도포 4일 후, 도포 8일 후, 도포 12일 후에 Chromameter CR-400(Minolta, Japan)을 이용하여 침착 부위의 피부 밝기에 대한 기기측정과 DSLR을 이용하여 사진 촬영을 실시하였다. 측정값은 최대값과 최소값을 제외한 3회의 평균값을 구하여 평가하였으며, 침착 부위의 피부 밝기 개선이 높을수록 각질 박리 개선 효과가 있음을 나타낸다. 결과는 하기 표 5에 나타내었다.Twenty test tubes of 20 women aged 20 to 50 years were coated with 1.5% solution of DHA (Dihydroxyacetone, Sigma Aldrich, USA) for 8 hours, and then the nutritional cream of Preparation Example 2 was applied to the test site twice daily . After 4 days of application, 8 days after application, 12 days after application, photographs were taken using instrument Chromameter CR-400 (Minolta, Japan) and skin lightness of DSLR and DSLR. The measured values were evaluated by three mean values excluding the maximum value and the minimum value. The higher the improvement in the skin brightness of the deposition site, the better the improvement of the exfoliation. The results are shown in Table 5 below.
표 5에 나타난 바와 같이, 본 발명에 따른 영양크림을 사용한 경우 각질 박리 개선 효과가 있는 것으로 나타났다.As shown in Table 5, when the nutritional cream according to the present invention was used, it was found that the exfoliation of the skin was improved.
제제예Formulation example 1: 의약 제제의 제조 1: Manufacture of pharmaceutical preparations
1. 정제의 제조1. Preparation of tablets
여지핵 추출물 0.2㎎Leaf Nuclear Extract 0.2 mg
옥수수전분 100㎎100 mg of corn starch
유 당 100㎎100 mg of milk
스테아린산 마그네슘 2㎎ 2 mg of magnesium stearate
상기의 성분을 혼합한 후, 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조하였다.After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.
제제예Formulation example 2: 화장품의 제조 2: Manufacture of cosmetics
1. 영양크림의 제조1. Manufacture of nutritional cream
하기 조성과 같이, 여지핵 추출물을 유효성분으로 포함하는 영양크림을 통상의 방법에 따라 제조하였다.As in the following composition, a nutritional cream containing a fleece nuclear extract as an active ingredient was prepared by a conventional method.
여지핵 추출물 0.2중량%0.2% w / w nuclear extract
베타-1,3-글루칸 5.0 중량%Beta-1,3-glucan 5.0 wt%
밀납 10.0 중량%Wax 10.0 wt%
폴리솔베이트60 1.5 중량%Polysorbate 60 1.5 wt%
피이지 60 경화피마자유 2.0 중량%≪ tb > < tb > < tb >
솔비탄세스퀴올레이트 0.5 중량%0.5% by weight of sorbitan sesquioleate
유동파라핀 10.0 중량%Liquid paraffin 10.0 wt%
스쿠알란 5.0 중량%Squalane 5.0 wt%
카프릴릭/카프릭트리글리세라이드 5.0 중량%Caprylic / capric triglyceride 5.0 wt%
글리세린 5.0 중량%Glycerin 5.0 wt%
부틸렌글리콜 3.0 중량%3.0% by weight of butylene glycol
프로필렌글리콜 3.0 중량%3.0% by weight of propylene glycol
트리에탄올아민 0.2 중량%0.2% by weight triethanolamine
방부제 0.05 중량%Preservative 0.05 wt%
색소 0.05 중량%0.05% by weight of pigment
향료 0.05 중량%0.05% by weight fragrance
정제수 to 100 중량%Purified water to 100%
제제예Formulation example 3: 피부 외용제의 제조 3: Preparation of external preparation for skin
1. 연고의 제조1. Manufacture of ointment
하기 조성과 같이, 여지핵 추출물을 유효성분으로 포함하는 연고를 통상의 방법에 따라 제조하였다.As in the following composition, an ointment containing a fleece extract as an active ingredient was prepared by a conventional method.
여지핵 추출물 0.5 중량%Leaf Nuclear Extract 0.5 wt%
베타-1,3-글루칸 10.0 중량%Beta-1,3-glucan 10.0 wt%
밀납 10.0 중량%Wax 10.0 wt%
폴리솔베이트60 5.0 중량%Polysorbate 60 5.0 wt%
피이지 60 경화피마자유 2.0 중량%≪ tb > < tb > < tb >
솔비탄세스퀴올레이트 0.5 중량%0.5% by weight of sorbitan sesquioleate
바셀린 5.0 중량%Vaseline 5.0 wt%
유동파라핀 10.0 중량%Liquid paraffin 10.0 wt%
스쿠알란 5.0 중량%Squalane 5.0 wt%
쉐어버터 3.0 중량%SHARE BUTTER 3.0 wt%
카프릴릭/카프릭트리글리세라이드 5.0 중량%Caprylic / capric triglyceride 5.0 wt%
글리세린 10.0 중량%Glycerin 10.0 wt%
프로필렌글리콜 10.2 중량%Propylene glycol 10.2 wt%
트리에탄올아민 0.2 중량%0.2% by weight triethanolamine
방부제 0.05 중량%Preservative 0.05 wt%
색소 0.05 중량%0.05% by weight of pigment
향료 0.05 중량%0.05% by weight fragrance
정제수 to 100 중량%Purified water to 100%
Claims (8)
A composition for improving atopy comprising an extract of a fleece nucleus as an active ingredient.
여지핵 추출물은 여지핵을 물 및 유기용매로 이루어진 군에서 선택된 하나 이상으로 추출하여 얻은 추출물인, 아토피 개선용 조성물.
The method according to claim 1,
Wherein the fleece nuclear extract is an extract obtained by extracting a fleece nucleus with at least one selected from the group consisting of water and an organic solvent.
유기용매는 탄소수 1 내지 5의 저급 알코올, 에틸아세테이트, 또는 아세톤을 포함하는 극성용매; 에테르, 클로로포름, 벤젠, 헥산 또는 디클로로헥산을 포함하는 비극성용매; 또는 이들의 혼합용매인, 아토피 개선용 조성물.
3. The method of claim 2,
The organic solvent may be a polar solvent containing a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone; A nonpolar solvent comprising ether, chloroform, benzene, hexane or dichlorohexane; Or a mixed solvent thereof.
조성물은 아토피 개선용 의약, 화장료 또는 식품 제조를 위한 것인, 아토피 개선용 조성물.
The method according to claim 1,
Wherein the composition is for the manufacture of a medicament for the improvement of atopy, a cosmetic preparation or a food product.
A composition for improving skin texture comprising an extract of a fleece nucleus as an active ingredient.
조성물은 피부결 개선용 화장료 또는 식품 제조를 위한 것인, 피부결 개선용 조성물.
6. The method of claim 5,
Wherein the composition is a cosmetic composition for improving skin texture or a composition for improving skin texture.
A composition for accelerating the activity of dermal stem cells comprising a fernaceous nuclear extract as an active ingredient.
조성물은 피부줄기세포 증식 또는 활성 촉진, 또는 피부줄기세포의 줄기세포성 유지용 화장료 제조를 위한 것인, 피부줄기세포 활성 촉진용 조성물.
8. The method of claim 7,
Wherein the composition is for the production of cosmetics for stimulating proliferation or activity of dermal stem cells or for maintaining the stem cell of dermal stem cells.
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