KR20150136899A - Bio sensor having nano gap - Google Patents
Bio sensor having nano gap Download PDFInfo
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- KR20150136899A KR20150136899A KR1020140064587A KR20140064587A KR20150136899A KR 20150136899 A KR20150136899 A KR 20150136899A KR 1020140064587 A KR1020140064587 A KR 1020140064587A KR 20140064587 A KR20140064587 A KR 20140064587A KR 20150136899 A KR20150136899 A KR 20150136899A
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- South Korea
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- nanogap
- electrodes
- nanoparticles
- biosensor
- biosensor according
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
Description
The present invention relates to a biosensor having a nanogap, and more particularly, to a biosensor for sensing a specific biomaterial existing in an organism through a nanogap.
Generally, a biosensor is a device for detecting a specific biomaterial such as an antigen-antibody of a protein, an enzyme of a DNA or a microorganism through a biological reaction between them or with them. Such a biosensor has a method of detecting by chemical, optical and electrical methods. Among them, recently, an electric method has been spotlighted because of its simple structure of equipment and low loss of sensed signals.
Specifically, in the biosensor of the electrical method, a connecting material that causes a biological reaction with the specific biomaterial is connected to each of the electrodes between a pair of facing electrodes, and the specific biomaterial contacts the connecting material The electrodes are connected to each other through a reaction with each other, so that the specific biomaterial is detected by an electrical signal generated from a voltage supplied from the outside.
At this time, since the electrodes are substantially made of biomaterials having high insulation characteristics, when the distance between the electrodes is relatively large in units of microns, the electrical signal is not generated. Therefore, It is necessary to form the gap between the electrodes so as to be very narrow as nano size so that the electrical signal can be detected by a specific bio material.
However, when the gap between the electrodes is formed to be very narrow as a nano size, the electrical signal is basically very weakly generated due to the material insulation characteristic of the specific bio material. Therefore, There is a problem in that it is difficult to distinguish whether it is due to an external factor such as biomaterial or other noise.
The present invention provides a biosensor having a nanogap capable of amplifying an electrical signal generated when sensing a biomaterial.
According to an aspect of the present invention, a biosensor includes a pair of electrodes, a connector, and nanoparticles.
The electrodes are supplied with a voltage having a nanogap between them. The connector connects the first marker positioned at each of the electrodes in the nanogap to each of the adjacent electrodes. The first marker is coupled to the bio-material that is introduced into the nanogap from the outside. The nanoparticles are positioned in the nanogap, and a second marker, which is bonded to the biomaterial, is connected to the surface.
The nanoparticles according to one embodiment may be made of an insulating material. Specifically, the nanoparticles may include metal oxides in which the cation is trivalent or tetravalent.
The size (W) of the nanoparticle according to one embodiment may be in the range of 0.1G? W? 25G when the nanogap is G.
The linker according to an embodiment may include any one selected from the group consisting of protein G, protein A, polyethylenimine, and carbonyldiimidazole .
The connector according to another embodiment may comprise an immobilized enzyme or a self assembled monolayer.
The connector according to another embodiment may include a carboxyl group or an amine group contained in each of the electrodes.
Any one of the first and second markers and the biomaterial according to an embodiment may include an antibody and the other may include an antigen.
According to another aspect of the present invention, there is provided a biosensor comprising a pair of electrodes, a connector and nanoparticles.
The electrodes are supplied with a voltage having a nanogap between them. The connector is connected to each of the electrodes in the nanogap, and is coupled to the bio-material flowing into the nanogap from the outside. The nanoparticles are positioned in the nanogap, and a reactor reacting with the biomaterial is connected to the surface.
The connector according to an embodiment includes any one selected from the group consisting of poly-L-lysine, probe oligonucleotide, oligopeptide, and carbonyldiimidazole. can do.
The connector according to another embodiment may include a combination of nickel-nitrilotriacetic acid and gold or a self assembled monolayer.
The biomaterial according to an embodiment may include DNA in a foreign body, and the reactor may include complementary DNA (cDNA).
The biomaterial according to another embodiment may include a microorganism, and the reactor may include a lectin.
According to the biosensor having a nanogap of the present invention, a bio-material flowing into a nanogap between a pair of electrodes to which a voltage is supplied connects the electrodes through nanoparticles positioned in the nanogap, The generated electrical signal can be amplified. As a result, the bio-material can be precisely distinguished and detected through the amplified electrical signal, and reproducibility can be stably ensured.
FIG. 1 is a configuration diagram conceptually showing a biosensor according to an embodiment of the present invention.
2 is a graph for explaining an effect of amplifying an electrical signal when sensing a bio material through the biosensor shown in FIG.
FIG. 3 is a configuration diagram conceptually showing a biosensor according to another embodiment of the present invention.
FIG. 4 is a configuration diagram conceptually showing a biosensor according to another embodiment of the present invention.
Hereinafter, a biosensor according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It should be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but on the contrary, is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. Like reference numerals are used for like elements in describing each drawing. In the accompanying drawings, the dimensions of the structures are enlarged to illustrate the present invention in order to clarify the present invention.
The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.
The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a part or a combination thereof is described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.
Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.
FIG. 1 is a block diagram conceptually showing a biosensor according to an embodiment of the present invention. FIG. 2 is a block diagram illustrating the effect of electrical signal amplification when sensing a bio material through the biosensor shown in FIG. Graph.
Referring to FIGS. 1 and 2, a
The
At this time, the
The
Here, the
In order to connect the
Alternatively, the
The
According to this configuration, the
Therefore, it is necessary that the
When the
This is because the
2, when the voltage is increased to the
Accordingly, it is possible to analyze the sensed bio-material 10 by calculating electrical characteristic values such as resistance, impedance and dielectric constant using the current value amplified through the
On the other hand, the
As described above, the
In addition, since the detection limit can be increased by amplifying the sensed current value using the
Further, in the state where the
FIG. 3 is a configuration diagram conceptually showing a biosensor according to another embodiment of the present invention.
3, a
The
Alternatively, the
The
Thus, when the
1, the schottky effect proceeds through the
Therefore, as in the embodiment with reference to FIG. 1, by securing a resolution that is increased according to the concentration through the amplified current value, it is possible not only to detect the
FIG. 4 is a configuration diagram conceptually showing a biosensor according to another embodiment of the present invention.
4, a
The
The
When the
1, when the voltage is gradually increased to the
Accordingly, as in the embodiment with reference to FIG. 1, by securing a resolution that is increased according to the concentration through the amplified current value, it is possible not only to detect the
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the appended claims. It will be understood that the present invention can be changed.
As described above, in the biosensor of the present invention, a specific biomaterial such as an antigen-antibody, a DNA or an enzyme of a microorganism of a protein flowing into a nanogap between a pair of electrodes to which a voltage is supplied is located in the nanogap By connecting the electrodes to each other through nanoparticles and amplifying an electrical signal generated through the nanoparticles, the biomaterial can be accurately and stably detected.
10: Biomaterials 20: Foreign DNA
30:
110, 210 and 310:
120, 220, 320: connector 122: first marker
130, 230, 330: nanoparticles 132: second marker
232, 332: reactor
Claims (15)
A connector positioned at each of the electrodes in the nanogap to connect a first marker, which is coupled to the bio-material introduced into the nanogap from the outside, to each of the adjacent electrodes; And
And a nanoparticle positioned on the nanogap, the nanoparticle having a second marker coupled to the surface, the nanoparticle being coupled to the biomaterial.
A coupling unit connected to each of the electrodes in the nano gap and coupled with a bio material introduced into the nanogap from the outside; And
And a nanoparticle positioned in the nanogap, the nanoparticle being connected to a surface of the reactor reacting with the biomaterial.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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KR1020140064587A KR20150136899A (en) | 2014-05-28 | 2014-05-28 | Bio sensor having nano gap |
PCT/KR2015/005070 WO2015182918A1 (en) | 2014-05-28 | 2015-05-21 | Bio-sensor having nano-gap |
Applications Claiming Priority (1)
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KR1020140064587A KR20150136899A (en) | 2014-05-28 | 2014-05-28 | Bio sensor having nano gap |
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KR1020140064587A KR20150136899A (en) | 2014-05-28 | 2014-05-28 | Bio sensor having nano gap |
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WO (1) | WO2015182918A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101880862B1 (en) * | 2017-11-10 | 2018-07-23 | (주) 비비비 | Iron Oxide Nanoparticle Complex And Biosensor Using The Same |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112689759A (en) * | 2018-08-09 | 2021-04-20 | Bbb有限公司 | Biosensor using magnetic nanoparticles, and detection apparatus and detection method using biosensor |
US20220099615A1 (en) * | 2019-01-18 | 2022-03-31 | Universal Sequencing Technology Corporation | Devices, Methods, and Chemical Reagents for Biopolymer Sequencing |
Family Cites Families (5)
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US20120037515A1 (en) * | 2009-04-15 | 2012-02-16 | TheStateof Oregonactingbyand throughthestateBoard ofHigherEducationon behalf of thePortlandstateUniv | Impedimetric sensors using dielectric nanoparticles |
KR20110128754A (en) * | 2010-05-24 | 2011-11-30 | 한국생명공학연구원 | Electrical biosensor for detecting infinitesimal sample |
KR20120038804A (en) * | 2010-10-14 | 2012-04-24 | 한국생명공학연구원 | Diagnostic method for detecting virus using nanogap electrodes and metal nanoparticles |
KR101779611B1 (en) * | 2011-01-20 | 2017-09-18 | 엘지전자 주식회사 | Cartridge for detecting target antigen and method for detecting target antigen using the same |
US9435800B2 (en) * | 2012-09-14 | 2016-09-06 | International Business Machines Corporation | Sample assembly with an electromagnetic field to accelerate the bonding of target antigens and nanoparticles |
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2014
- 2014-05-28 KR KR1020140064587A patent/KR20150136899A/en not_active Application Discontinuation
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- 2015-05-21 WO PCT/KR2015/005070 patent/WO2015182918A1/en active Application Filing
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101880862B1 (en) * | 2017-11-10 | 2018-07-23 | (주) 비비비 | Iron Oxide Nanoparticle Complex And Biosensor Using The Same |
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