KR20150085451A - A composition for treating or preventing obesity comprising extracts of pear - Google Patents

Abstract

The invention pears (Pyrus The present invention relates to a composition for the treatment or prevention of obesity, which comprises, as an active ingredient, a pear water extract which is a fruit of serotina , and specifically a water extract of apricot after the pear juicing. In particular, the extract of Baeckum, which is an active ingredient of the present invention, is advantageous economically because it uses a by-product, which is a byproduct produced after a brewing juice, and has an excellent effect of inhibiting weight gain and inhibiting lipogenesis of fat cells It is not only excellent in the treatment and prevention of obesity but also can be widely used for treating or preventing obesity since natural substance is used as an active ingredient and no side effects are caused.

Description

TECHNICAL FIELD [0001] The present invention relates to an anti-obesity composition,

The present invention relates to a composition for treating or preventing obesity, which comprises a natural substance having an anti-obesity activity as an active ingredient.

Various lifestyle-related diseases and chronic degenerative diseases due to nutritional overcrowding, environmental pollution, lack of exercise, increased stress due to the improvement of economic level are becoming big problems in our society. Of these, obesity is the disease most recently focused on.

Obesity refers to the fact that the energy intake exceeds the energy expenditure so that the surplus energy causes a quantitative and numerical increase of the adipocyte so that the fat tissue accumulation state or genetic factor, (Devlin et al., 2000; Spiegelman and Flier (1996)). In addition, it is important to understand the relationship between energy balance and physical activity.

The World Health Organization (WHO) has already recognized obesity as a disease to be treated and treated it as a global nutritional problem (World Health Organization (1998)). Currently, the number of obese patients is on the rise, and the World Health Organization recently reported that over one billion adults worldwide are overweight, and at least 300 million of them are obese. According to the 2007 National Health and Nutrition Examination Survey data released by the Ministry of Health and Welfare, the obesity population with a body mass index (BMI) of 25 or more steadily increases and the public health is seriously threatened (Ministry of Health and Welfare (2007 )).

Obesity is particularly prevalent in morbidity and mortality compared to other diseases and has been found to be associated with various complications of adult diseases. For example, obesity patients have been reported to have a higher mortality rate compared to those with normal weight, twice as much as mild illness, 1.6 times as for cerebrovascular disease and 1.8 times as for coronary artery disease. Currently, various drug therapies including dietary therapy, exercise therapy, diuretic agents and diarrhea have been tried, but their use is limited temporarily and limitedly due to the occurrence of adverse effects such as nutritional imbalance, immunity depression and depression.

Regarding the above-mentioned obesity, studies on localization (Adipogensis) are under way. The localization refers to a process in which adipocytes are differentiated from fat precursor cells to accumulate fat, and the localization is known to cause metabolic diseases such as obesity, diabetes, fatty liver and coronary heart disease. The adipocyte functions not only as an energy reservoir but also as an endocrine organs, which secretes a protein hormone called adipocytokine to perform various functions for maintaining energy homeostasis. However, excessive adipocyte differentiation and unbalanced energy metabolism may cause abnormal gene expression and signal transduction in adipocytes, leading to adipocytokine secretion abnormalities.

Leptin, adiponectin, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and resistin have been reported as adipocytekine. For example, leptin, an appetite regulating protein, decreases appetite and increases energy expenditure, and the adiponectin increases expression during adipocyte differentiation, thereby increasing liver and muscle tissue insulin sensitivity and increasing fatty acid oxidation, (2007), Meller et al. (2006)), a decrease in the expression level of adiponectin and obesity induction have been reported to be related to obesity (Ambati et al. (Okamoto et al. (2006)).

In adipocytes, lipid metabolism occurs through lipolysis and synthesis (adipogenesis). During the lipid synthesis, the preadipocyte undergoes proliferation and differentiation to differentiate into mature adipocytes, And lipid droplets are formed in the cells. The transcription factors that induce the differentiation process of the adipocytes include adipocyte determination and differentiation dependent factor 1 (ADD1 / SPEBP1c), PPARγ (peroxisome proliferator activated receptor γ) and C / EBPα (CCAAT enhancer binding protein α) has been reported. The transcription factor induces expression at different time points during adipocyte differentiation and regulates the expression of adipocyte specific genes through mutual interaction and gradually induces the activation of fat metabolism and the differentiation of adipocytes (Kersten, 2001 Spiegelman and Flier, 1996).

Bone and Tartaglia (2000) reported that the obesity treatment regimes for eliminating obesity and maintaining proper body weight include controlling appetite, inhibiting fat digestion and absorption, increasing energy expenditure, and controlling lipid metabolism. Currently commonly used obesity drugs are serotonin and norepinephrine reuptake inhibitors which inhibit sibutramine (ReductilR), which acts on the hypothalamus to lower appetite and energy consumption, and lipase activity There is orlistat (XenicalR), a drug that helps prevent the hydrolysis of cholesterol and triglyceride and excretes unoxidized fat into the stomach.

However, sibutramine has been reported to increase blood pressure and be used with caution in patients with cardiovascular disease. In addition, orlistat has been reported to induce gastrointestinal disturbances, adiposity, fecal incontinence, and fat-soluble vitamin absorption.

Therefore, it is required to develop a drug for obesity which has a high anti-obesity effect and a low side effect among natural products expected to minimize the possibility of a safety problem. That is, it is required to develop a substance derived from a natural substance, which inhibits the differentiation process of the adipocyte and has a low side effect.

KR 100947278 B KR 1020120043936 A KR 1020120016961 A

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art, and it is an object of the present invention to provide an anti-obesity composition comprising a natural substance as an active ingredient.

In order to accomplish the above object, the present invention provides a pharmaceutical composition for treating or preventing obesity comprising an extract of Pseudomonas sp. As an active ingredient. The pellet extract may be a pellet extract.

In addition, the present invention provides a food composition for improving or preventing obesity comprising an extract of a pear as an active ingredient. The pellet extract may be a pellet extract. Examples of the food composition include a food, a food additive, a beverage or a drink additive, and preferably a health functional food.

In order to accomplish the above object, the present invention also provides a method for separating ovaries from a ship, A step of squeezing the separated ovary by a pressing method; Separating the juice produced after the juice to obtain juice produced in the juicing process; And a step of adding water to the resultant bran and extracting it for 24 hours. The present invention also provides a pharmaceutical composition for treating or preventing obesity, comprising the extract of Bacillus subtilis as an active ingredient.

In order to accomplish the above object, the present invention also provides a method for separating ovaries from a ship, A step of squeezing the separated ovary by a pressing method; Separating the juice produced after the juice to obtain juice produced in the juicing process; And a step of adding water to the resultant bran and extracting it for 24 hours. The present invention also provides a food composition for improving or preventing obesity, comprising the extract of Bacillus subtilis as an active ingredient.

The inventors of the present invention have been studying the anti-obesity activity of a fruit that has been studied in a limited manner, unlike herbs and other medicinal plants which have been studied variously, and it is recognized that, in abundance of abundant fish in Naju area, , And water extract of Pueraria japonica showed anti-obesity activity compared with other solvent extracts, specifically, in experimental animals, the weight gain And lipogenesis, and thus, the present invention has been completed.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a pharmaceutical composition for the treatment or prevention of obesity which comprises an extract of Leucocephalus as an active ingredient

The pear is a fruit of a pear, and the portion of the fruit that can be eaten is about 80%, the content of water is 85% to 88%, the main ingredient is a carbohydrate, and the sugar such as fructose and sucrose, , And organic acids such as citric acid, as well as components such as vitamin B and vitamin C, and is rich in fiber and minerals. It is mainly effective against bronchial diseases, and helps defecation and diuretic action.

The pear ( Pyrus) serotina ) is a vernacular word in the rose and pear tree. It is about 2m to 3m in height. The leaves are ovate with sawtooth, and three white flowers bloom in spring. The pear tree is also referred to as a tree or a tree. There are several kinds of wild varieties such as perennials, southern pear, and luminous trees. Recently, cultivated varieties include.

The plant extract, specifically, the extract of the present invention or the extract of Leptospira, which is an extract of the present invention, can be prepared by extracting with a extraction solvent or extracting with a extraction solvent and fractionating the extract with a fraction solvent.

The extraction solvent may be water or water which is a solvent that can be used as a food, and is most excellent in anti-obesity effect.

Since the present invention is for producing a substance having an anti-obesity effect as an active ingredient of a therapeutic composition or a food composition used for medicinal use or food from a natural substance having a very low possibility of side effects, Ethanol, and a mixed solvent thereof. Preferably, the water may be one having an excellent anti-obesity effect.

The extract of the present invention may be one produced by a conventional method of producing an extract from a field plant or an extract of a fruit of the above-mentioned terrestrial plant, specifically, a cold extraction method, a warm extraction method or a heat extraction method, A device, an ultrasonic grinding extractor or a fractionator may be used.

In addition, the extract of the present invention may be a fraction obtained by adding a fraction solvent to the crude extract extracted with the solvent and then further fractionating the extract. The fraction solvent may be a solvent selected from the group consisting of water, ethyl acetate, ether, chloroform, benzene, hexane, methylene chloride, and mixed solvents thereof.

The thus-prepared extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a vacuum concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying.

In one embodiment of the present invention, the embryo extract can be obtained by the following method.

Specifically, the extract of the present invention comprises a step of separating the ovary of the embryo from the embryo; A step of squeezing the separated ovary by a pressing method; And adding water to the balsam produced after the juice and extracting it for 24 hours, more particularly, separating the non-fermented ovary from the boat; Removing the oily juice produced in the juicing process by squeezing the ova separated from the ovary by compression; And extracting water by adding water to the scallop and heating at 120 ° C for 1 hour. The extracted extract is filtered, concentrated under reduced pressure at 40 ° C for 1 hour and 40 minutes, It can be freeze-dried in a freezer at 70 ° C for 24 hours.

The above-mentioned method for producing extract of Baechu Water may have an anti-obesity effect as compared with extracts using other solvents, specifically, Puerariae extract.

The present invention relates to a pharmaceutical composition for the treatment or prevention of obesity comprising an extract of a pear as an active ingredient. The puddle extract may preferably be a puddle extract.

In the present invention, the term "obesity" refers to a phenomenon in which the balance of energy in the human body is collapsed due to genetic factors, environmental factors, mental factors, lifestyle such as eating habits and lack of exercise, This means that the surplus energy surpasses the expenditure and causes the accumulation of adipose tissue by quantitative and numerical increase of adipocyte. Obesity is particularly prevalent in morbidity and mortality compared to other diseases and has been found to be associated with various complications of adult diseases.

The composition for treating or preventing obesity comprising the pear water extract of the present invention as an active ingredient may contain the pear extract in an amount of 0.001 to 99.99% by weight, preferably 0.1 to 99% by weight, based on the total weight of the composition.

The composition for treating or preventing obesity, which comprises the above-mentioned pear-water extract as an active ingredient, is a water extract of a ship, which is a natural product used for a long period of time, does not cause side effects and is excellent in improving or preventing obesity It has an excellent effect in terms of point.

The pharmaceutical composition for treating or preventing obesity comprising the pear water extract of the present invention as an active ingredient can be directly applied to humans.

The pharmaceutical composition for treating or preventing obesity, which comprises the above-described pear extract as an active ingredient, may contain the pear extract alone as an active ingredient, and may further contain additional components, that is, a pharmaceutical composition Acceptable or nutritionally acceptable carrier, excipient, diluent or subcomponent.

More particularly, the pharmaceutical composition for the treatment or prevention of obesity, which comprises the above-mentioned swine extract as an active ingredient, may further contain, in addition to the active ingredient, a nutritional supplement, a vitamin, an electrolyte, a flavoring agent, a colorant, a heavy stabilizer, a pectic acid and its salt, A salt thereof, an organic acid, a protective colloid thickener, a pH adjuster, a stabilizer, a preservative, a glycerin, an alcohol, and a carbonating agent used in a carbonated beverage. The carrier, excipient or diluent may be selected from lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acicia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline In the group consisting of celluloses, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, But the present invention is not limited thereto. Any conventional carrier, excipient or diluent may be used. These components may be added to the active ingredient, i.e., the podiatric extract, either independently or in combination.

The medicinal composition for treating or preventing obesity, which comprises the above extract of pear as an active ingredient, may contain 0.001% by weight to 99.9% by weight, preferably 0.1% by weight to 99% by weight, 1% by weight to 50% by weight. In addition, the content of the additional ingredient may be added in the range of 0.1 to 20 parts by weight per 100 parts by weight of the composition for treating or preventing obesity.

In addition, when the pharmaceutical composition for treating or preventing obesity containing the extract of the present invention is used as an active ingredient, it may be formulated with conventional fillers, extenders, binders, disintegrants, surfactants, anticoagulants, lubricants, wetting agents, Preservatives, and the like, and they can be used either orally or parenterally.

Particularly, solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations are prepared by adding to the active ingredient, the pear extract, at least one excipient such as starch, calcium carbonate calcium carbonate, sucrose or lactose, and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, have.

In addition, the formulation of the pharmaceutical composition for treating or preventing obesity, which comprises the pear water extract of the present invention as an active ingredient, may be in a preferable form depending on the method of use, and may be an immediate, sustained or delayed release A method known in the art can be employed to formulate the drug. Examples of the specific formulations include granules, powders, syrups, liquids, suspensions, tablets, injections, main tablets, cataplasma, capsules, soft or hard gelatin capsules and the like.

Furthermore, pharmaceutical compositions for the treatment or prevention of obesity comprising the poultry extract of the present invention as an active ingredient can be prepared using appropriate methods known in the art or can be prepared according to the methods described in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA). ≪ / RTI >

The dosage of the pharmaceutical composition for the treatment or prevention of obesity, which comprises the extract of the present invention as an active ingredient, is suitably selected by a person skilled in the art in consideration of the administration method, age, sex and weight of the recipient, . For example, the pharmaceutical composition for the treatment or prevention of obesity comprising the pear water extract of the present invention as an active ingredient may be administered at a dose of 0.0001 mg / kg to 1000 mg / kg, 0.01 mg / kg to 100 mg / kg. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

In addition, the pharmaceutical composition for treating or preventing obesity, which comprises the pear water extract of the present invention as an active ingredient, may further comprise an effective ingredient, that is, a compound having a known therapeutic effect on obesity or a preventive effect, And may be included in an amount of 5 to 200 parts by weight based on 100 parts by weight of the active ingredient.

In order to attain the above object, the present invention provides a food composition for preventing or improving obesity, which comprises the extract of the present invention as an active ingredient.

As used herein, the term " food " means a natural product or a processed product containing one or more nutrients. Preferably, it means that the food can be directly eaten through a certain degree of processing. Health functional foods, beverages, food additives, and beverage additives.

The food of the present invention includes, for example, various foods, beverages, gums, tea, a vitamin complex, a health functional food, and the like. In addition, in the present invention, the food may contain special nutritional foods (e.g., crude oil, spirits, infant food, etc.), meat products, fish products, tofu, jelly, noodles (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, fruits, vegetables, beverages, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.). The food, the health functional food, the beverage, the food additive and the beverage additive can be produced by a usual production method.

In the present invention, the health functional food refers to a food group imparted with added value to function or express the function of the food by physical, biochemical, biotechnological techniques, etc., or to control the biological defense rhythm of the food composition, Means a food that is designed and manufactured so that the weight control function related to recovery and the like is sufficiently expressed in living bodies.

The health functional food may include food-acceptable food supplementary additives, and may further include suitable carriers, excipients and diluents conventionally used in the production of health functional foods.

In the present invention, beverage is a generic term for drinking or enjoying a taste, and is intended to include a health functional beverage. The beverage may contain various flavors or natural carbohydrates as an additional ingredient, such as ordinary beverages, without any particular limitation, other than that the beverage contains the pour water extract as an active ingredient at an indicated ratio as an essential ingredient have.

Examples of such natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and Xylitol, sorbitol, and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate may be generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the food composition of the present invention. In addition, the composition of the present invention can be used for the production of natural fruit juice, fruit juice drink, Can also be added.

In addition to the above, the food composition of the present invention can be used as a flavoring agent such as a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. These components can be used independently or in combination. The proportion of such additives is not so critical, but may be selected in the range of 0 to 2,000 parts by weight per 100 parts by weight of the extract of the present invention.

In the present invention, the health functional beverage includes a beverage group to which added value is imparted so that the function of the beverage functions to a specific purpose using physical, biochemical, biotechnological techniques, etc., Means a beverage which is processed by being designed so that the body control function related to recovery and the like is sufficiently expressed to a living body.

The health functional beverage is not particularly limited to the other components except for containing the pear extract of the present invention as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages .

Examples of such natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and xylitol , Sorbitol, and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.

In addition, in the food composition for preventing or improving obesity which comprises the pear water extract as an active ingredient, the effective ingredient, i.e., the amount of the pear water extract may be 0.01 to 15% by weight of the total food, 0.0 > g < / RTI > to 5 g, preferably 0.3 to 1 g.

It has been confirmed that the pear water extract of the present invention is excellent in anti-obesity effect, specifically, weight loss effect, appetite suppressing effect, inhibition of fat cell size increase inhibition and lipid absorption inhibition effect. Particularly, , The extract of Baeckum was excellent in anti-obesity effect, and it was confirmed that the composition comprising the extract of Baei extract of the present invention as an active ingredient showed excellent obesity treatment or prevention effect.

The pear water extract of the present invention is excellent in the treatment and prevention effects of obesity, as well as being excellent in the ability to inhibit weight gain and inhibit lipogenesis of adipocytes compared with the conventional organic solvent, Since the pear water extract, which is an effective ingredient, especially the pear extract, is a byproduct which is a byproduct produced after pear juicing, it has an economically advantageous effect. The pear as a raw material of the effective ingredient has been used for a long time as a food, Since safety is ensured because it is a safe extraction solvent and toxicity due to solvent is not a problem, the present invention can contribute to activation of regional economy and local farmer's income in Chonnam region where abundant production of bait is abundant, It will be very big.

1 to 3 are graphs showing experimental results comparing the anti-obesity effects of the extract of each of the extractants according to an embodiment of the present invention. FIG. 2 is a graph showing changes in dietary intake during the rearing period of an experimental animal according to an embodiment of the present invention, and FIG. 3 is a graph showing changes in the rearing period of the experimental animal according to an embodiment of the present invention Kg body weight. The abscissa in FIG. 1 and FIG. 2 means the measurement time, that is, the time elapsed from the start of the experiment, and the ordinate means the body weight (g) or the dietary intake (g). The abscissa of FIG. 3 represents each experiment group, and the ordinate represents the dietary efficiency (%).
FIG. 4 to FIG. 8 are graphs showing experimental results comparing the anti-obesity effects of the extract of each of the extractants according to an embodiment of the present invention. FIG. FIG. 5 is a graph showing liver weight versus body weight measured after a breeding period according to an embodiment of the present invention. FIG. 6 is a graph showing the weight (g) of a fat tissue according to an embodiment of the present invention. FIG. 7 is a graph showing the total cholesterol content (TC) in plasma measured after a breeding period according to an embodiment of the present invention, and FIG. 8 is a graph showing the total triglyceride content (TG) (LDL) measured after the breeding period. The horizontal axis of the graph indicates the kind of the extracts of the control group or the experimental group, that is, the extract of Prunus persicae (PPH) and the extract of Prunus persica (PPE) It means a dose that is, 200 mg / kg (200) and 400 mg / kg (400), and the vertical axis represents the measured value.
9 to 10 are graphs showing the results of comparison of anti-obesity effects of the extracts of the extractants according to an embodiment of the present invention. FIG. 9 is a graph showing the fat-producing ability of adipocytes according to an embodiment of the present invention The upper part is the photograph of the plate, the lower part is the microscope photograph of the cell, the left side is the control group, the middle part is the group treated with 100 μg / ml extract, and the right part is 250 μg / ml FIG. 9B shows the aqueous extract of Bacillus subtilis; FIG. 10 shows the effect of the anti-obesity effect of the Bacillus subtilis extract on lipid-differentiated cells (PBS) and sample (bamboo extract) added (μg / ml), and the vertical axis represents the proliferation of the cells based on the control group 10A shows an extract of Auburn water, and FIG. 10B shows an extract of Auburn ethanol aqueous solution.

Hereinafter, examples of the present invention will be described. The following examples are for illustrative purposes only and are not intended to limit the scope of the present invention. However, the following Preparation Examples and Examples are merely illustrative examples for the purpose of facilitating understanding of the present invention, and can be modified into various other forms, and the scope of the present invention is not limited to the following Examples.

< Example  1> Preparation of Boar Extract

The pear samples were washed three times from the fresh water in the neighboring orchards in Naju area, Jeollanam-do, and then the non-viable part of the ovary was removed. The pear was collected and crushed with a 1,600 kg hammer mill with ovary removed, 176 kg of bran were collected. 20 kg of water was added to 1 kg of the collected bait, and hot water extraction was performed at 120 ° C for 1 hour. The bran extract was filtered using a filter paper, concentrated under reduced pressure for 1 hour and 40 minutes, and lyophilized in a freezer at -70 ° C to prepare a total of 320 g of Bacillus edulis extract.

In addition, a 50% ethanol aqueous solution (alcohol) was added to 1 kg of the bran, and the mixture was extracted at 40 ° C for 24 hours, followed by the same filtration process, decompression concentration process and lyophilization process to produce a total of 275 g of aqueous ethanol extract .

The extract prepared by the above method was stored at -20 ° C in a freezer until used in the experiment.

Example  2: of pear water extract Anti-obesity  effect Measure 1

Obesity animal models were prepared by using 5-week-old male db / db obese mice (C57BL / Ks db / db mice, Central Laboratories, Korea) The anti - obesity effect of the extracts of A. aquaculture and aqueous ethanol solution was measured.

2-1. Breeding of experimental animals

Experimental animals were treated with 5% week-old db / db obese mice (C57BL / Ks db / db mice) and adapted to a diet containing 45% kcal fat based on AIN-93G three days prior to the start of the experiment .

The control group (Control), 200 mg / kg of the extract group (PPH 200), 400 mg / kg of the extract of Pueraria japonica (PPH 400), 200 mg / kg of ethanol extract of bovine plant and PPE 200 mg / kg administration group (PPE 400). The dose of extract was orally administered at the same time (10:00 am) for 6 weeks at a dose of 200 mg / kg or 400 mg / kg, and the diet and water were supplied fresh every day. The animal breeding room maintained temperature, humidity, And maintained for 9 weeks.

2-2. weight, Dietary intake , Water intakes and Dietary efficiency  Measure

The body weight, the dietary intake, the water intake, and the dietary efficiency of the experimental animal of Example 2-1 were measured once a week by subtracting the remaining amount from the previous day supply. The results are shown in Figs. 1 to 3 and Table 1 . Body weight in the following Table 1 and Fig. 1 was measured by body weight gain (g) every one week from the body weight (23.1 g (control group and water extract) or 23.0 g (ethanol aqueous solution extract) (G) / food (g)). FIG. 2 shows the food intake. FIG. 3 is a graph showing the body weight gain (g) divided by the average food intake intake (g)).

division Weight per week (g) One 2 3 4 5 6 7 8 Control (blue squares) 25.1 25.8 26.9 28.4 29.4 30.9 34.0 35.9 PPH 200 (red square) 25.1 25.5 26.4 27.2 27.9 28.0 28.0 27.6 PPH 400 (green triangle) 24.7 25.4 26.1 26.3 27.3 28.0 27.6 27.4 PPE 200 (purple) 25.1 25.6 26.5 27.2 28.9 32.0 32.7 33.8 PPE 400 (blue) 24.5 25.2 26.2 27.3 28.6 29.3 29.8 31.0

As shown in FIG. 1 and Table 1, in the case of the body weight, the ethanol extract of Bacillus thunbergii did not show any significance until 200 mg, whereas the Bacillus thunbergii extract significantly inhibited the body weight gain , And it was confirmed that it had an excellent effect of inhibiting weight gain compared with the group administered 400 mg of ethanol extract. In addition, as shown in FIG. 3, in the case of the body weight dietary efficiency, the ethanol extract of Bacillus subtilis did not have a significant meaning as compared with the control group, whereas in the case of Bacillus subtilis extract, It was confirmed that it was helpful for.

From the above results, it was confirmed that the extract of Bombyx mori was superior to the aqueous extract of Bombyx mori.

2-3. Measurement of inhibition of fat production

In order to confirm the effect of inhibiting lipogenesis, body fat depression effect and blood lipid - lowering effect were confirmed. The body fat reduction effect was measured by sacrificing the experimental animals after sacrificing the experimental animals for 8 weeks, cutting the abdomen of the experimental animals, and removing the fat around the epididymis and measuring the weight , Blood lipid-lowering effect After sacrificing the above-mentioned experimental animals, blood was separated from the abdominal aorta of an experimental animal, and then serum was separated using a centrifuge. The neutral fat and total cholesterol content in the separated serum were measured with an Auto Chemisry Analyzer (Beckman coulter / AU480). The results of the respective tests are shown in FIGS. 4 to 8. FIG.

As shown in FIG. 4 and FIG. 5, the weight of the adipose tissue extract was significantly lower than that of the control group. On the other hand, the ethanol extract of the perilla ethanol showed a somewhat lower inhibitory effect on the adipocyte formation, mg showed a superior inhibitory effect on fat production compared with the control group administered with 400 mg of aqueous ethanol extract. On the other hand, in the case of liver weight measurement relative to body weight, similar weight to that of the control group was confirmed in all experimental groups, and it was confirmed that the bamboo extracts as expected were safe regardless of the solvent type.

As a result of the analysis of the serum of the experimental animals, the amount of total triglyceride, total cholesterol and LDL cholesterol in the serum was significantly lowered in the extract of Bombyx mori. Whereas in the case of the extract of aqueous ethanol extract, the degree of decrease in total triglyceride content and LDL cholesterol content was not significant. Therefore, it was confirmed from the above results that Baechu-water extract having water as an extraction solvent is preferable in terms of anti-obesity effect in the production of Baechu extract.

Example  3: of pear water extract Anti-obesity  effect Measure 2

3T3-L1 adipocyte precursor cells were used for cell study to determine the anti-obesity effect of the pear water extract. The 3T3-L1 adipose precursor cells were purchased from the American Type Culture Collection (ATCC; USA). The pre-cultured 3T3-L1 adipose precursor cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Hyclone) containing 100 units / ml penicillin, 100 μg / ml streptomycin and 10% newbone calf serum % Oxygen and 5% CO ₂ . The 3T3-L1 adipose precursor cells were subcultured by supplementing with fresh medium every 2 days

 The adipocyte differentiation induction of the lipid precursor cells was carried out by the following method.

First, lipid precursors were seeded at a concentration of 5 × 10 ⁴ cells / well in a 6-well plate and cultured to complete confluent state. After 4 days, the differentiation inducing substances insulin 5 μg / ml, 1 μM dexamethasone The medium was replaced with DMEM medium containing 0.5 mM isobutylmethyl xanthine and 10% FBS, and the extract was added at a concentration of 100 μg / ml or 250 μg / ml, respectively. After 2 days from the exchange of the medium, the medium was replaced with DMEM medium containing insulin 5 μg / ml and 10% FBS again with fresh medium supplemented with bamboo extract, And supplemented with fresh DMEM containing 10% FBS, and after 4 days, experiments on the cell differentiation ability were carried out. After induction of differentiation for 8 days, the cells were washed with PBS, and the cells washed with PBS were fixed with 10% formalin solution at room temperature for 30 minutes, and then washed once with distilled water. After washing, the cells were treated with 4% Oil Red O solution for 1 hour, washed once with 60% isopropanol, and observed for adipocytes using a microscope. The results were shown in FIG.

In addition, the ability to inhibit the proliferation of adipocytes was confirmed by MTT assay.

Specifically, 3T3-L1 adipose precursor cells inducing differentiation into zona pellucida were cultured in a 96-well plate, and the extract was added at a concentration of 100 μg / ml or 250 μg / ml. MTT [ The adipocyte proliferative capacity was measured using a solution of 3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide.

More specifically, the lipid precursor cells differentiated into the adipocytes were added to the lipid precursor cells in an amount of 100 μg / ml or 250 μg / ml, compared with the control group in which the lipid extract was not added. The number of cells was confirmed by the method using MTT solution, and the results are shown in FIG.

As shown in Fig. 9, it was confirmed that the production of fat droplets was remarkably reduced in the experimental group (Fig. 9A) treated with the extract of Akoi than that of the control group not treated with the sample. According to the throughput of Akoi extract, / ml treatment group (right), the degree of red staining was blurred to the extent that it could be grasped visually by the naked eye, and the degree of fat production was remarkably reduced even in the photographs observed in adipocytes . From the above results, it was confirmed that the extract of Opuntia ficus-indica inhibits lipogenesis. On the other hand, in the experimental group (FIG. 9B) treated with the extract of aqueous ethanol solution of bamboo, it was confirmed that the degree of formation of the fat droplets was slightly decreased. However, in the case of the whole photographs, the degree of decrease thereof was hardly confirmed. The results showed that the effect of the extracts on the lipid - lowering ability of adipocytes was different depending on the solvent.

As shown in FIG. 10, it was confirmed that 100 μg / ml or 250 μg / ml of Leek extract (FIG. 10a) was significantly reduced compared to the control, and that Leek extract was 250 μg / ml treatment, the adipocyte proliferation was significantly inhibited as compared with the treatment with 100 μg / ml of bamboo extract. On the other hand, when treated with the extract of aqueous ethanol solution of bamboo ethanol (FIG. 10b) And there was no difference in the effect depending on the dose. Thus, it was confirmed that there was almost no effect of suppressing the production of giant cell. As a result, it was confirmed that the extract of Baeckum extract significantly inhibited the differentiation into adipocyte, while the extract of Baecke ethanol aqueous solution hardly inhibited the differentiation into adipocyte.

Manufacturing example : Exhaustion  Formulation with water extract

Manufacturing example  One. Sanje  Produce

Prune water extract powder 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients were mixed and filled in an airtight container to prepare powders.

Manufacturing example  2. Preparation of tablets

Prune water extract powder 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to the usual preparation method of tablets.

Manufacturing example  3. Preparation of capsules

Prune water extract powder 10 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components were mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Manufacturing example  4. Liquid  Produce

Prune water extract powder 20 mg

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added to purified water in accordance with the usual liquid preparation method and dissolved, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100 ml with purified water, And sterilized to prepare a liquid preparation.

Manufacturing example  5. Manufacture of health functional foods

Prune water extract powder 1000 mg

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

24.8 mg of magnesium chloride

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a component suitable for a health functional food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above components may be mixed And then granules are prepared and used in the manufacture of health functional foods according to conventional methods.

Claims (4)

Separating the ovary of the ship from the ship; A step of squeezing the separated ovary by a pressing method; Separating the juice produced after the juice to obtain juice produced in the juicing process; And extracting the extract obtained by adding water to the resulting extract for 24 hours, as an active ingredient. Separating the ovary of the ship from the ship; A step of squeezing the separated ovary by a pressing method; Separating the juice produced after the juice to obtain juice produced in the juicing process; And a step of adding water to the resultant bran and extracting it for 24 hours. The composition for improving or preventing obesity according to claim 1, A food composition for improving or preventing obesity comprising a pear water extract as an active ingredient. The method of claim 3,
Wherein the pear water extract is an extract of Bombyx mori.

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