KR20140134436A - Mixing apparatus for culture medium - Google Patents

Abstract

The present invention relates to an apparatus to mix culture medium to a culture of animal and plant cells used to produce raw materials of medicine like proteins, and a pouch to mix culture medium. The apparatus of the present invention comprises: a body; a stretch pocket installed in the body and allowed to pressurize some part of the culture medium pocket using a working fluid pressure such that the culture medium is mixed in the culture medium pocket installed in the body; a working fluid medium supplying apparatus which supplies working fluid inside the stretch pocket; and a control unit which enables the working fluid supplying apparatus to receive control signals.

Description

Mixing apparatus for culture medium < RTI ID = 0.0 >

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture medium mixing apparatus and a culture medium mixing bag, and more particularly, to a culture medium mixing apparatus and a culture medium mixing bag which are mixed with a medium capable of culturing animal and plant cells for producing a raw material for pharmaceuticals, Device and a medium mixing bag.

In general, the culture industry for culturing an animal or a plant cell includes a culture medium mixing apparatus for mixing the medium (culture medium) so as to be compatible with the culture environment, a cell culture apparatus capable of culturing an animal or a plant cell in a culture medium, A separating and purifying device for separating the living body from the living body, and other genetic manipulating apparatus.

Recently, as a medium mixing apparatus used for bioprocess due to the growth of the culture industry of animal cells, disposable pouches with low possibility of continuous use and contamination can be applied.

In the conventional media mixer, an actuator such as a motor or a piston / cylinder is used to uniformly mix the medium contained in the disposable media bag. However, the capacity of the disposable media pocket is increased from several liters to tens of liters, As the number of the bag pockets to be mixed together increases, the size, specification, specification and performance of the required motors, actuators such as pistons / cylinders, and the like are increased, resulting in a significant increase in manufacturing and production costs of the apparatus.

In addition, the apparatus for mixing media using a motor or a piston / cylinder is slow in operation, is not efficient due to mechanical limitations, and is poor in mixing performance of the medium and wasteful energy. As a result, the performance of the product can not be improved.

In addition, the conventional culture medium mixing apparatus is capable of mixing only the culture medium. The user can mix the culture medium with the culture medium in the culture medium mixer, and thereafter, the culture medium must be cultivated in a separate culture apparatus by moving the culture medium bag. There was a hassle.

Disclosure of Invention Technical Problem [11] Accordingly, the present invention has been made to solve the above problems occurring in the prior art, and it is an object of the present invention to greatly improve the size, performance, efficiency and productivity of a device using a flexible pocket, It is an object of the present invention to provide a culture medium mixing apparatus and a culture medium mixing bag capable of culturing cells in a culture medium. However, these problems are exemplary and do not limit the scope of the present invention.

According to an aspect of the present invention, there is provided an apparatus for mixing media, comprising: a body; An elastic pocket provided on the body and capable of pressing a part of the discharge pouch using the pressure of the working medium so that a medium accommodated in the discharge pocket accommodated in the body can be mixed; A working medium supply device capable of supplying the working medium into the inside of the elastic pocket; And a controller for applying a control signal to the working medium supply device.

According to an aspect of the present invention, the elastic pockets include at least a left pocket for pressing a left portion of the at least fixed media bag, a right pocket for pressing a right portion of the inserted media bag, Wherein the control unit selects at least one of the left pocket, the right pocket and the center pocket to selectively supply the working medium alternately while alternately selecting the at least one of the left pocket, the right pocket and the center pocket, It may be to apply an alternate control signal to the working medium supply.

According to an aspect of the present invention, the working medium may be one selected from at least one of air, gas, water, oil, and combinations thereof.

In addition, the apparatus for mixing media according to an aspect of the present invention may further include a support plate installed between the media bag and the elastic pockets and supporting the media bag.

In addition, the apparatus for mixing media according to an embodiment of the present invention may further include a temperature controller installed on the support plate and heating or cooling the media bag.

According to an aspect of the present invention, the temperature regulating device includes at least a heater including a heat plate or a hot wire, a thermoelectric element, a water-cooled heat exchanger through which the refrigerant can circulate, an air-cooled heat exchanger using natural convection or a blowing fan, Or a combination of any of these.

In addition, the apparatus for mixing media according to the present invention may further comprise: a weight measuring sensor for measuring the weight of the culture bag; A temperature measurement sensor for measuring the temperature of the culture bag; An oxygen supply device for measuring the concentration of oxygen contained in the medium contained in the medium bag using an oxygen measurement sensor and supplying oxygen to the medium using an oxygen supply tube installed in the medium bag; An acid base supplying device for measuring the acidity of the culture medium accommodated in the culture medium bag using an acidity measurement sensor and supplying an acidic or basic substance to the culture medium using an acid base supply tube installed in the culture medium bag; And a nutrient supply device for measuring the nutrient concentration of the medium contained in the medium bag using the nutrient measurement sensor and supplying nutrients to the medium using the nutrient supply tube installed in the medium bag.

In addition, the culture medium blending bag according to the present invention includes: a casing capable of receiving a culture medium; And at least one elastic pocket provided inside the shell and capable of mixing the medium using the pressure of the working medium supplied from the outside through the working medium supply tube.

In addition, the medium mixing bag according to the present invention may further include a heater installed inside the shell and capable of heating the medium by receiving power from the outside through a power line.

Further, in accordance with an aspect of the present invention, there is provided a blending bag for a medium according to the present invention, the blending bag comprising: a pressure regulator provided on the outer shell, A sampling tube installed on the outer shell and installed so as to sample the inside of the culture medium; An oxygen supply pipe installed on the outer shell to supply oxygen to the medium; An acid base supply pipe provided on the outer shell and supplying an acidic or basic substance to the culture medium; And a nutrient supply pipe installed on the outer skin to supply nutrients to the medium.

According to some embodiments of the present invention as described above, it is possible to manufacture a high-performance device with low cost and time, and it is possible to increase the size of the device and to mix the medium of the large capacity pouch, The productivity can be greatly improved. Of course, the scope of the present invention is not limited by these effects.

1 is a cross-sectional view conceptually showing a medium mixing apparatus according to some embodiments of the present invention.
FIGS. 2 and 3 are cross-sectional views illustrating the operation of the elastic pocket of FIG. 1;
FIGS. 4 and 5 are cross-sectional views conceptually illustrating an operation process according to another embodiment of the elastic pocket of FIGS. 2 and 3. FIG.
6 is a cross-sectional view conceptually showing a medium mixing apparatus according to some other embodiments of the present invention.
7 is a perspective view conceptually showing a medium mixing bag according to some embodiments of the present invention.
8 is a perspective view conceptually showing a medium mixing bag according to some other embodiments of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The embodiments of the present invention are described in order to more fully explain the present invention to those skilled in the art, and the following embodiments may be modified into various other forms, It is not limited to the embodiment. Rather, these embodiments are provided so that this disclosure will be more thorough and complete, and will fully convey the concept of the invention to those skilled in the art. In the drawings, the thickness and size of each layer are exaggerated for convenience and clarity of explanation.

It is to be understood that throughout the specification, when an element such as a film, region or substrate is referred to as being "on", "connected to", "laminated" or "coupled to" another element, It will be appreciated that elements may be directly "on", "connected", "laminated" or "coupled" to another element, or there may be other elements intervening therebetween. On the other hand, when one element is referred to as being "directly on", "directly connected", or "directly coupled" to another element, it is interpreted that there are no other components intervening therebetween do. Like numbers refer to like elements. As used herein, the term "and / or" includes any and all combinations of one or more of the listed items.

Although the terms first, second, etc. are used herein to describe various elements, components, regions, layers and / or portions, these members, components, regions, layers and / It is obvious that no. These terms are only used to distinguish one member, component, region, layer or section from another region, layer or section. Thus, a first member, component, region, layer or section described below may refer to a second member, component, region, layer or section without departing from the teachings of the present invention.

Also, relative terms such as "top" or "above" and "under" or "below" can be used herein to describe the relationship of certain elements to other elements as illustrated in the Figures. Relative terms are intended to include different orientations of the device in addition to those depicted in the Figures. For example, in the figures the elements are turned over so that the elements depicted as being on the top surface of the other elements are oriented on the bottom surface of the other elements. Thus, the example "top" may include both "under" and "top" directions depending on the particular orientation of the figure. If the elements are oriented in different directions (rotated 90 degrees with respect to the other direction), the relative descriptions used herein can be interpreted accordingly.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a," "an," and "the" include singular forms unless the context clearly dictates otherwise. Also, " comprise "and / or" comprising "when used herein should be interpreted as specifying the presence of stated shapes, numbers, steps, operations, elements, elements, and / And does not preclude the presence or addition of one or more other features, integers, operations, elements, elements, and / or groups.

Hereinafter, embodiments of the present invention will be described with reference to the drawings schematically showing ideal embodiments of the present invention. In the figures, for example, variations in the shape shown may be expected, depending on manufacturing techniques and / or tolerances. Accordingly, the embodiments of the present invention should not be construed as limited to the particular shapes of the regions shown herein, but should include, for example, changes in shape resulting from manufacturing.

1 is a cross-sectional view conceptually showing a medium mixing apparatus 100 according to some embodiments of the present invention.

First, as shown in FIG. 1, a medium mixing apparatus 100 according to some embodiments of the present invention includes a main body 10, a flexible pocket 20, a working medium supply device 30, A control unit 40, a support plate 50, and a temperature control unit 60.

Here, the body 10 may be a kind of frame body formed so that the paper bag 1 can be supported at a predetermined height from the ground.

The body 10 may be manufactured in various shapes such as a work table shape, a cabinet shape, and the like, and is merely conceptually illustrated in FIG. 1. However, those skilled in the art Various modifications and changes are possible.

The disposable diaper 1 may be made of a hard or soft material. However, in FIG. 1, a disposable diaper bag made of a soft material is exemplified for convenience of explanation. In addition, a variety of synthetic resin or various metal materials (Container) of the present invention can be applied.

The stretchable pockets 20 are provided on the body 10 so that the stretchable pockets 20 are formed in the body 10 so that the medium C accommodated in the body pouch 1 can be mixed. Is a bag which can press a part of the paper bag (1) using pressure.

The elastic pockets 20 may be made of a flexible elastic material such as a thin film or a film of rubber, silicone, or urethane so as to be stretchable or contractible, and may be formed of a simple pouch, And the like.

The stretchable pockets 20 shown in Fig. 1 are provided with a left pocket 21 for pressing a part of the left side of the stationary bag 1 and a right pocket (not shown) for pressing the right part of the inserted bag 1 22).

The working medium supply device 30 is capable of supplying the working medium M to the inside of the elastic pocket 20. The working medium supply device 30 may be any of various compressors, compressors, working medium tanks, high pressure gas cylinders, . ≪ / RTI >

Such a working medium supply device 30 is an apparatus capable of quickly supplying or discharging the working medium M into the elastic pockets 20. The mechanical parts of the working medium feeding device 30, The description of a compressor, a compressor, a working medium tank, a high-pressure gas cylinder, and various control valves is well known and will not be described in detail.

Here, the working medium M may be air, and various types of gas and liquid such as gas, water, and oil may be applied.

The control unit 40 controls the operation medium supply device 30 so as to alternately supply one of the left pocket 21 and the right pocket 22 and alternately supply the working medium M, A signal can be applied.

The support plate 50 is installed between the paper bag 1 and the elastic pockets 20 and can be in direct contact with the paper bag 1, A hard metal panel can be applied. Here, in addition to such a metal panel, various hard or soft synthetic resin panels can be applied. However, the support plate 50 may be omitted depending on the type and the kind of the paper bag 1, the usage environment, and the like.

The temperature regulating device 60 is installed on the support plate 50, and is a device for heating or cooling the exhaust pouch 1.

Here, the temperature regulating device 60 may be a heater 61 including a heat plate or a heat line, as shown in FIG.

In addition, although not shown, the temperature regulating device 60 may include a thermoelectric element, a water-cooled heat exchanger capable of circulating the refrigerant, an air-cooled heat exchanger capable of using natural convection or a blowing fan, .

Figs. 2 and 3 are cross-sectional views illustrating the operation of the elastic pocket 20 of Fig.

As shown in FIGS. 2 and 3, the operation of the medium mixing apparatus 100 according to some embodiments of the present invention will be described in more detail. For example, as shown in FIG. 2, When the left pocket 21 is applied with the left pocket extension signal to the working medium supply device 30, the working medium M is quickly supplied only to the inside of the left pocket 21, And a support plate 50 provided above the left pockets 21 can press a part of the left side of the paper bag 1.

At this time, the control unit 40 can apply the right pocket contraction signal to the working medium supply device 30, so that the inner working medium M of the right pocket 22 is quickly discharged, The size of the pocket 22 is contracted and the support plate 50 provided above the right pocket 22 can decompress a portion of the right side of the medium bag 1.

Therefore, the culture medium C in the culture medium bag 1 can be partially vortexed in the right downward direction from above the left side of the culture medium bag 1 due to gravity, and can be uniformly mixed in the primary.

3, when the control unit 40 applies a right pocket extension signal to the working medium supply device 30, the working medium M is quickly moved to only the inside of the right pocket 22 So that the size of the right pocket 22 is extended and the support plate 50 provided above the right pocket 22 can press the right part of the discharge pouch 1.

At this time, the control unit 40 can apply the left pocket contraction signal to the working medium supply device 30, so that the internal working medium M of the left pocket 21 is quickly discharged, The size of the pocket 21 is contracted and the support plate 50 provided above the left pocket 21 can decompress a part of the left side of the culture bag 1.

Therefore, the culture medium C in the culture medium bag 1 is partially vortexed while flowing in the left downward direction from the right side of the culture medium bag 1 due to gravity, so that the culture medium C can be mixed evenly.

Then, the controller 40 may alternately supply the working medium M to the left pocket 21 and the right pocket 22 differentially, and repeat the mixing process described above.

Figs. 4 and 5 are cross-sectional views conceptually showing an operation process according to another embodiment of the elastic pockets 20 of Figs. 2 and 3.

4 and 5, the elastic pockets 20 include a left pocket 21 for pressing at least a part of the left side of the media bag 1 which is at least stuck, a right pocket 21 for the left side of the media bag 1 A right pocket 22 for pressing a part of the bag 1 and a center pocket 23 for pressing the center of the loaded bag 1.

The control unit 40 may be configured to select at least one of the left pocket 21, the right pocket 22 and the center pocket 23 to alternately supply the working medium M differentially, An alternate control signal can be applied to the supply device 30. [

4 and 5, the operation of the medium mixing apparatus according to some embodiments of the present invention will be described in more detail. For example, as shown in FIG. 4, When the left and right pocket extension signals are applied to the working medium supply device 30, the working medium M is quickly supplied only to the inside of the left pocket 21 and the right pocket 22, And the support plate 50 provided above the left pockets 21 and the right pockets 22 can press the left part and the right part of the culture pouch 1 have.

At this time, the control unit 40 can apply a central pocket contraction signal to the working medium supply device 30, so that the internal working medium M of the center pocket 23 is quickly discharged, The size of the pocket 23 is contracted and the support plate 50 provided above the central pocket 23 can reduce the central portion of the medium bag 1.

Therefore, the culture medium C in the culture medium bag 1 can be partially vortexed while flowing in the downward direction from the left and right sides of the culture medium bag 1 due to gravity, so that the culture medium C can be mixed evenly.

5, when the control unit 40 applies a central pocket extension signal to the working medium supply device 30, only the working medium M is quickly moved to the inside of the center pocket 23 So that the size of the central pocket 23 is extended and the support plate 50 provided above the central pocket 23 can press a central portion of the medium bag 1.

At this time, the control unit 40 can apply the left and right pocket contraction signals to the working medium supply device 30, and thus the inner working medium M of the left pocket 21 and the right pocket 22 The left pockets 21 and the right pockets 23 are contracted in size and the support plate 50 provided above the left pockets 21 and the right pockets 22 is pushed out of the discharge pockets 1 Can be decompressed.

Therefore, the medium C inside the medium bag 1 is partially vortexed while flowing in the left lower side and the right down side from above the center of the medium bag 1 by gravity, so that the medium C can be evenly mixed with the second medium.

The control unit 40 may alternately supply the working medium M to the left pockets 21, the right pockets 22 and the center pockets 23 while alternately repeating the mixing process .

6 is a cross-sectional view conceptually showing a medium mixing apparatus 200 according to some other embodiments of the present invention.

6, the medium mixing apparatus 200 according to some other embodiments of the present invention includes a weight measuring sensor S1 for measuring the weight of the medium bag 1, The oxygen concentration sensor 1 measures the concentration of oxygen contained in the medium C accommodated in the medium bag 1 by using a temperature sensor S2 measuring the temperature of the medium 1 An oxygen supply device 70 for supplying oxygen to the medium C using an oxygen supply pipe 71 installed in the medium feed port 1 and a pH sensor An acidic base supply device 80 and a nutrient measurement sensor S5 for measuring acidity and supplying an acidic or basic substance to the medium C using the acidic base supply pipe 81 provided in the medium bag 1, , The nutrient concentration of the medium (C) contained in the culture medium bag (1) was measured, And a nutrient supply device 90 for supplying nutrients to the medium C using the nutrient supply pipe 91 provided in the paper bag 1. In addition, the paper bag 1 may be provided with a pressure regulator 2 and a sampling tube 3.

Accordingly, the user mixes the culture medium C in the medium bag 1 and controls the weight measurement sensor S1, the temperature measurement sensor S2, the oxygen measurement sensor S3, the acidity measurement sensor Temperature, acidity and nutrients necessary for cell culture are actively formed on the medium C of the medium bag 1 by using the nutrient measurement sensor S5 and S4 ) Can be cultured.

Therefore, according to the medium mixing device 200 according to some other embodiments of the present invention, since the cells can be cultured in the medium C while mixing the medium C, , It is possible to prevent the redundant investment of the apparatus, and to maximize the productivity and the efficiency of the apparatus.

7 is a perspective view conceptually showing a medium mixing bag 1000 according to some embodiments of the present invention.

7, the medium mixing bag 1000 according to some embodiments of the present invention includes a sheath 1010 capable of accommodating the medium C and a sheath 1010 provided inside the sheath 1010, 1022 that can mix the medium C using the pressure of the working medium M supplied from the outside through the working medium supply tube 1023. [

Accordingly, the medium mixing bag 1000 according to some embodiments of the present invention may be configured such that external devices are connected to the working medium supply pipe 1023 to simultaneously or alternately supply the working medium to the flexible pockets 1021 and 1022 Whereby the inner medium C can be evenly mixed.

8 is a perspective view conceptually showing a medium mixing bag 2000 according to some other embodiments of the present invention.

8, the medium mixing bag 2000 according to some other embodiments of the present invention is installed inside the envelope 1010 and receives power from the outside through a power line, A pressure regulator 1040 installed in the outer casing 1010 to regulate the inner air pressure and a pressure regulator 1040 installed in the casing 1010 to regulate the temperature of the inner medium C An oxygen supply pipe 2060 installed on the outer casing 1010 for supplying oxygen to the medium C and a gas supply pipe 2060 installed on the outer casing 1010 to supply oxygen to the medium C, An acid base supply pipe 2070 for supplying an acidic or basic substance to the medium C and a nutrient supply pipe 2080 for supplying nutrients to the medium C, .

Therefore, the medium mixing bag 2000 according to some embodiments of the present invention can be used to mix the inner medium C uniformly using the elastic pockets 1021 and 1022, It is possible to provide convenience to the user, prevent redundant investment of the device, and maximize the productivity and the efficiency of the device.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

1: Badge pocket
10: Body
C: Badge
M: Operating medium
20: Elastic pocket
30: Working medium supply device
40:
100, 200: medium mixing device
21: Left pocket
22: Right pocket
23: Central pocket
50: Support plate
60: Temperature control device
61: heater
S1: Weighing sensor
S2: Temperature sensor
70: oxygen supply device
71: oxygen supply pipe
80: Acid Base Supply
81: Acid base supply pipe
90: Nutrient supply
91: Nutrient supply line
2: Pressure regulator
3: Sampling tube
1000, 2000: mixed medium bag
1010: Sheath
1021, 1022: elastic pocket
1023: Working medium feeder
2030: Heater
1040: Pressure regulator
2050: Sampling tube
2060: Oxygen supply pipe
2070: Acid base supply line
2080: Nutrient supply line

Claims (10)

Body;
An elastic pocket provided on the body and capable of pressing a part of the discharge pouch using the pressure of the working medium so that a medium accommodated in the discharge pocket accommodated in the body can be mixed;
A working medium supply device capable of supplying the working medium into the inside of the elastic pocket; And
A control unit for applying a control signal to the working medium supply device;
. The method according to claim 1,
The elastic pockets include a left pocket for pressing at least a part of the left side of the storage bag, a right pocket for pressing a right portion of the storage bag, a central pocket for pressing the center of the storage bag, Or more,
Wherein the control section selects at least one of the left pocket, the right pocket and the center pocket and applies the alternate control signal to the working medium supply device so as to alternately supply the working medium alternately. The method according to claim 1,
Wherein the working medium is selected from at least one of air, gas, water, oil, and a combination thereof. The method according to claim 1,
A support plate provided between the media bag and the elastic pockets, for supporting the media bag;
Further comprising: 5. The method of claim 4,
A temperature regulating device installed on the support plate for heating or cooling the exhaust pouch;
Further comprising: 6. The method of claim 5,
The temperature regulating device may include at least one selected from a heater including at least a heat plate and a hot wire, a thermoelectric element, a water-cooled heat exchanger through which the refrigerant can circulate, an air convection heat exchanger using natural convection or a blowing fan, Wherein the culture medium is mixed with the culture medium. The method according to claim 1,
A weighing sensor for measuring the weight of the culture bag;
A temperature measurement sensor for measuring the temperature of the culture bag;
An oxygen supply device for measuring the concentration of oxygen contained in the medium contained in the medium bag using an oxygen measurement sensor and supplying oxygen to the medium using an oxygen supply tube installed in the medium bag;
An acid base supplying device for measuring the acidity of the culture medium accommodated in the culture medium bag using an acidity measurement sensor and supplying an acidic or basic substance to the culture medium using an acid base supply tube installed in the culture medium bag; And
A nutrient supply device for measuring the nutrient concentration of the medium contained in the medium bag using the nutrient measurement sensor and supplying nutrients to the medium using the nutrient supply tube installed in the medium bag;
Further comprising: A sheath capable of receiving the medium; And
At least one elastic pocket provided inside the shell and capable of mixing the medium with the pressure of the working medium supplied from the outside through the working medium supply tube;
And a mixing bag. 9. The method of claim 8,
A heater installed inside the outer shell and capable of heating the medium by receiving power from the outside through a power line;
Further comprising: 9. The method of claim 8,
A pressure regulator provided on the outer shell for regulating an internal air pressure;
A sampling tube installed on the outer shell and installed so as to sample the inside of the culture medium;
An oxygen supply pipe installed on the outer shell to supply oxygen to the medium;
An acid base supply pipe provided on the outer shell and supplying an acidic or basic substance to the culture medium; And
A nutrient supply pipe installed on the outer skin to supply nutrients to the medium;
Further comprising:

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