KR20130088224A - Skin external composition containing drumstick tree seed extract - Google Patents

Abstract

PURPOSE: An external use skin composition is provided to contain a large amount of carotenoid and amino acid ingredients using a germinated drumstick tree seed extract or a drumstick tree seed extract by supercritical extraction and to enhance a whitening effect. CONSTITUTION: An external use skin composition contains 0.01-95 wt% of a drumstick tree seed extract as an active ingredient, which is prepared by a supercritical extraction. The drumstick tree seeds are germinated seeds. The composition is used for skin whitening.

Description

Skin external composition containing Drumstick tree seed extract

The present invention relates to a composition for external application for skin containing an extract of Moringa seeds, and more particularly, by preparing an extract from germinated Moringa seeds or by preparing a Moringa seed extract using a subcritical extraction method. Compared to the extract of Linga seed provides a moringa seed extract containing a variety of types and a large amount of potent ingredients, and by using the same relates to an external composition for skin that can exhibit higher efficacy.

Human skin color is determined by several factors, such as the activity of melanocytes (melanocytes) that make melanin pigment, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigments in and outside the body, such as carotenoids and bilirubin. In particular, a black pigment called melanin produced by the action of various enzymes such as tyrosinase in melanocytes is the most important factor. The formation of melanin pigment is influenced by genetic factors, physiological factors related to hormone secretion, stress, and environmental factors such as ultraviolet irradiation. Melanin is present in the skin to protect the body from ultraviolet rays, but it is known that the excessive production of melanin promotes pigmentation and skin aging and also plays a major role in causing skin cancer. Ascorbic acid, kojic adcid, arbutin, hydroquinone, hudroquinone , Glutathione or derivatives thereof, and substances having tyrosinase inhibitory activity have been used in cosmetics and pharmaceuticals, which have insufficient whitening effects, safety problems for the skin, and when formulated in cosmetics. Its use is limited due to stability problems.

Domestic Publication No. 2009-0046011

Therefore, the present inventors can provide an excellent whitening effect of the extract of Moringa seeds among natural extracts, in particular, the extract of the germinated Moringa seeds has a higher content of carotenoids, amino acids, etc. than the general Moringa seeds, extraction method By using the subcritical extraction method was able to extract a wider variety of types and a large amount of potency ingredients to provide a more excellent whitening effect was completed the present invention.

Accordingly, it is an object of the present invention to provide a composition for external application for skin that exhibits various skin improvement effects including the whitening effect by containing the Moringa seed extract.

In order to achieve the above object, the present invention provides an external composition for skin containing the extract of the germinated Moringa seed as an active ingredient.

The present invention also provides an external composition for skin containing an extract of Moringa seed prepared by the subcritical extraction method as an active ingredient.

The present invention also provides a whitening skin external composition comprising the extract of the moringa seed germinated or Moringa seed extract prepared by the subcritical extraction method as an active ingredient.

The composition according to the present invention comprises a higher amount of carotenoid and amino acid components as compared with the conventional Moringa seed extract by conventional extraction method by using the germinated Moringa seed extract or Moringa seed extract by subcritical extraction method. Can provide higher whitening effect.

The external preparation composition for skin of the present invention contains an extract of Moringa seed as an active ingredient.

Moringa seed used in the present invention is a seed of Moringa tree, Moringa tree is a plant of Moringaceae Family, Moringa Genus, the scientific name is Moringa Oleifera (aka Moringa Pterygosperma), In Tamil, it is also called Murunggai. Moringa is a legume, a tropical tree that consumes leaves and fruits as well as the entire tree, and contains more than 90 nutrients such as amino acids, minerals and vitamins.

In particular, the water-soluble proteins contained in Moringa seeds help to purify contaminants in water. When these water-soluble proteins are applied to the skin, they detoxify the skin by purifying contaminants. In addition, it contains a large amount of carotenoids, vitamins and minerals such as beta-carotene, rutin, etc., to improve the appearance of dull skin brightening effect.

Subcritical moringa seed extract used in the present invention is extracted from the seeds of Moringa, preferably from the germinated Moringa seeds. Moringa seeds in germination state contain more potent ingredients necessary for life phenomena compared to the state before germination, which can provide stronger efficacy.

Although germination conditions of Moringa are not specifically limited, It can germinate and use by the following method. In case of outdoor soil, germinate after 2 ~ 4 weeks in sunny place at 25 ℃. In addition, in indoor conditions, after soaking in room temperature water for about 24 hours and transferred to the incubator for 48-72 hours, the room temperature should be kept warm above 25 ℃.

In addition, the composition of the present invention contains Moringa seed extract prepared by Subcritical Water Extraction as an active ingredient. At this time, the moringa seed may use both the moringa seed and the germinated moringa seed before germination.

In conventional supercritical extraction methods, the density of supercritical fluid is similar to liquid, and the viscosity and diffusion coefficient are similar to gas. Therefore, if the supercritical fluid is used as the extraction solvent, the micropore of the plant easily penetrates and has an appropriate molecular density, thereby obtaining an extract of the desired substance by surrounding the molecules of the desired substance and exiting. At this time, the material that can be used as an extraction solvent by making it into a supercritical state must first be non-toxic, and there must be no danger of explosion since the supercritical state is high temperature / high pressure, and the solvent must be excellent as an extraction solvent. If these conditions are met, any material can be extracted into a supercritical fluid.

On the other hand, the subcritical technology used in the present invention is used as an extraction solvent in a state in which the solvent does not exceed the critical point, and depending on what solvent is selected, the subcritical technology can perform the same performance as the supercritical extraction. Use water as In addition, supercritical carbon dioxide should not be used to extract polar substances, so organic solvents should be used as co-solvents. However, subcritical water has better extraction performance, and the range of change in operating conditions is large, making it impossible to extract both nonpolar and polar substances. It is possible. In addition, in the case of extraction with subcritical water, the hydrolysis reaction can also proceed.

Moringa seed extract by the subcritical extraction method used in the present invention may be specifically prepared as follows. After preparing moringa seed, the active ingredient is extracted by decomposing moringa seed at a high pressure of 50 to 200 MPa, preferably 100 MPa, using a subcritical extraction device. At pressures below 50 MPa, the active ingredients are not sufficiently extracted, and at pressures above 200 MPa, the amount of extraction of the active ingredients is less than that of the high pressure. After extraction, it is possible to prepare a subcritical moringa seed extract by filtering once in a filter having a 1.2 μm pore, once in a filter having a 0.45 μm pore, and once in a filter having a 0.2 μm pore to remove foreign substances and microorganisms. .

The composition of the present invention is 0.01-95.0% by weight, preferably 5.0-95.0% by weight of the extract of germinated Moringa seeds or subcritical Moringa seed extract prepared by the same method as the total weight of the composition, more preferably May be contained in an amount of 10.0 to 90.0% by weight. If the content of the extract is less than 0.01% by weight, it is difficult to expect the skin efficacy of the ingredient, and when the content exceeds 95.0% by weight, the effect is no longer increased significantly, the raw material efficiency is reduced.

In addition, the composition of the present invention may further contain a known substance exhibiting a whitening effect. Specifically, it may further contain one or more selected from the group consisting of ascorbyl glucoside, licorice extract, arbutin, ascorbic acid, kojic acid and niacinamide, which is contained in 0.5 to 5.0% by weight based on the total weight of the composition. . This is because if less than 0.5% by weight is absorbed by the skin is difficult to show the efficacy, if greater than 5.0% by weight is toxic to the skin or the skin stickiness is greatly increased, it is difficult to show the value as a cosmetic.

The composition of the present invention exhibits the detoxifying effect of purifying contaminants in the skin to clean the skin, and can improve the brightening of the dull skin to realize a whitening effect.

In addition, the composition of the present invention may help to improve the overall condition of the skin in addition to the skin whitening effect, provides excellent antioxidant power, anti-aging effect through skin elasticity enhancement or skin wrinkle improvement, skin moisturizing improvement effect , Anti-inflammatory, acne and atopic dermatitis improvement, sebum control effect, pore contraction effect, keratin improvement effect, skin convergence or trimming effect, improvement of complexion through blood circulation, skin irritation effect, homeostasis improvement etc. It can provide an overall improvement of the skin condition.

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) and non- It may be provided in the form of an ionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin whitening effect.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

Example 1 Preparation of General Germinated Moringa Seed Extract

The germinated Moringa seeds were placed in 70% purified water and 30% alcohol and left at 25 ° C. for 6 hours to extract the active ingredients. After extraction, the filter was filtered once with a 3 μm pore and once with a filter with a 1 μm pore to remove foreign substances. Finally, microorganisms were removed with a sterilization filter to obtain a general Moringa seed extract.

Example 2 Preparation of Subcritical Moringa Seed Extract

Moringa seeds, which are not germinated, were extracted using a subcritical extractor at high pressure of 100 MPa. After extraction, the filter was filtered once with a 1.2 μm pore, once with a 0.45 μm pore, and once with a 0.2 μm pore to remove foreign substances and microorganisms, thereby obtaining a subcritical moringa seed extract.

Example 3 Preparation of Subcritical Germinated Moringa Seed Extract

The germinated Moringa seeds were extracted with active ingredients at a high pressure of 100 MPa using a subcritical extractor. After extraction, the filter was filtered once with a 1.2 μm pore, once with a 0.45 μm pore, and once with a 0.2 μm pore to remove foreign substances and microorganisms, thereby obtaining a subcritical moringa seed extract.

Comparative Example 1 Preparation of General Moringa Seed Extract

Moringa seeds, which are not germinated, were placed in 70% purified water and 30% alcohol and left at 25 ° C. for 6 hours to extract active ingredients. After extraction, the filter was filtered once with a 3 μm pore and once with a filter with a 1 μm pore to remove foreign substances. Finally, microorganisms were removed with a sterilization filter to obtain a general Moringa seed extract.

Experimental Example 1 Extraction of Moringa Seed Extract and Comparison of Contents of Active Ingredients According to Germination Conditions

The amino acid content of each of the Moringa seed extracts of Examples 1 to 3 and Comparative Example 1 was analyzed using the following amino acid analysis method (OPA method). The results are shown in Table 1 below.

<Amino Acid Assay (OPA Method)>

1) HPLC conditions

Column: zorbax column (amino acid analysis only)

Mobile phase:

A = 1.36 g Sodium acetate trihydrate + 100 μl triethylamine → 500 mL diaphragm → pH 7.2 (controlled with acetic acid) → THF 1.5 mL

B = 1.36 g sodium acetate trihydrate / 100 ml H ₂ O → pH 7.2 + methanol 200 ml + ACN 200 ml

Flow rate: 0.5 ml / min

Injection: Injection program for online derivatization *

Detector: 338 nm

Gradient: Online Program

2) Reagent Preparation

Amino acid standard solution: 10 mg / 100 ml H ₂ O such as aspartic acid, glutamic acid, proline, glycine, alanine, valine

-OPA reagent: Reagent made and supplied by HP, shelf life 6 months, ampoules

Borate buffer: 100 ml unit, required for amino acid development

amino acid Content (μg / mL) Generic Germinated Moringa Seed Extract
(Example 1) Subcritical Moringa Seed Extract
(Example 2) Subcritical Germinated Moringa Seed Extract
(Example 3) Common Moringa Seed Extract
(Comparative Example 1) Asp 0.54 0.57 0.69 0.33 Glu 0.55 0.52 0.88 0.24 Ser 0.40 0.41 0.52 0.30 His 0.31 0.32 0.24 - Gly 0.31 0.32 0.90 0.13 The 0.12 0.20 0.32 - Arg 0.20 0.31 0.45 0.15 Ala 0.20 0.20 0.30 0.17 Tyr 0.21 0.33 0.32 0.21 Cys 0.88 1.30 1.55 0.55 Val 0.28 0.26 0.55 - Met 0.43 0.42 0.72 0.52 Phe 0.40 0.32 0.29 0.30 Ile 0.31 0.28 0.35 0.25 Leu 0.19 0.38 0.63 0.10 Lys 0.29 0.44 0.52 0.32 Pro 0.44 0.54 0.60 0.51 all 6.06 7.12 9.83 4.08

As can be seen in Table 1, when compared to 100% of the amino acid content of the Moringa seed extract (Comparative Example 1) obtained by using the general extraction method Moringa seed extract (Example 2) of the germination state is 149%, Moringa seed extract prepared by the subcritical extraction method according to the present invention (Example 2) increased the total amino acid content to 174%, in particular germinated moringa seed extract extracted subcritically using the germinated seeds (Example 3) increased the amino acid content significantly to 241%.

Therefore, it can be confirmed that the moringa seed extract or the subcritical moringa seed extract of the germinated state used in the present invention contains more potent ingredients than the conventional moringa seed extract.

Reference Example 1 Preparation of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 and 2

According to the composition of Table 2 to prepare a cosmetic composition of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 and 2.

Ingredient (Unit: wt%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 1. Purified water To 100 To 100 To 100 To 100 To 100 2. Dipropylene Glycol 5.0 5.0 5.0 5.0 5.0 3. Butylene Glycol 5.0 5.0 5.0 5.0 5.0 4. Bio Sacolite Gum-1 4.0 4.0 4.0 4.0 4.0 5. General Germination Moringa Seed Extract (Example 1) 10.0 - - - - 6. Subcritical Moringa Seed Extract (Example 2) - 10.0 - - - 7. Subcritical Germination Moringa Seed Extract (Example 3) - - 10.0 - - 8. General Moringa Seed Extract (Comparative Example 1) - - - 10.0 - 9. Ethanol 5.0 5.0 5.0 5.0 5.0 10. Phenyl Trimethicone 0.2 0.2 0.2 0.2 0.2 11.Polyoxyethylene Hydrogenated Castor Oil 0.5 0.5 0.5 0.5 0.5 12. Preservatives Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount 13. Incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

<Manufacturing Method of Formulation Example and Comparative Formulation Example>

1) Mixing and dissolving the raw materials 1 to 8 of Table 2 to prepare a water part.

2) Dissolve the raw materials 9-13 of Table 2 at 50 ℃ in a separate container to prepare an alcohol part.

3) The alcohol part of step 2) is added to the water part of step 1) and mixed by stirring with an azimuth mixer.

4) Removing the bubbles of the mixture of 3) to prepare a cosmetic.

Test Example 2 Whitening Effect Test on Human Skin

Ten healthy women were attached with an opaque tape with five 1.5cm diameter holes on the upper forearm of the subject, and 1.5 ~ 2 times the UVB of the minimum Erythema Dose of each subject. Induce skin blackening by irradiating the skin, and the formulation Examples 1 to 4 and Comparative Formulation Examples 1 to 2 were applied to each hole daily, twice daily in the morning and evening, and then measured after 50 days using a color difference meter. It was.

The effect was judged by measuring the degree of blackness of the skin using a color difference meter (Minolta CR2002). L, a, and b colorimetric systems are used to display colors. In the present invention, "L" values representing skin darkness were calculated and analyzed (color values of unburned Korean skin are usually 50 to 70). L values were calibrated with a standard white plate, and measurements were made evenly by repeating the measurements five times or more per site.

When the test substance is effective, the L value is gradually increased, and the comparison between the test substances was calculated according to the following equation (1) by calculating the difference in skin color (ΔL) at the start and completion of the application. The results are shown in Table 3 below.

Test for whitening effect on human skin Test substance Whitening effect (? L) Formulation Example 1 1.88 Formulation Example 2 2.45 Formulation Example 3 3.77 Comparative Formulation Example 1 1.54 Comparative Formulation Example 2 0.62

As can be seen in Table 2, in the case of Formulation Example 1 containing the germinated Moringa seed extract, compared to Comparative Formulation Example 1 containing a general Moringa seed extract and Comparative Formulation Example 2 containing no Moringa seed extract It can be seen that the effect is high.

In addition, in the case of Formulation Examples 2 to 3 containing the Moringa seed extract prepared by subcritical extraction method, the whitening effect is more noticeable than Comparative Formulation Example 1 and Formulation Example 1 containing the Moringa seed extract obtained by using the general extraction method. It can be confirmed that high. In particular, in the case of Formulation Example 3 containing a subcritical germinated moringa seed extract, compared to Formulation Example 1 containing a subcritical moringa seed extract 1.5 times or more, compared to Comparative Formulation Example 1 using a general moringa seed extract By more than double the whitening effect can be confirmed.

Claims (7)

An external preparation composition for skin containing the extract of germinated moringa seed as an active ingredient. An external preparation composition for skin containing an extract of Moringa seed prepared by subcritical extraction as an active ingredient. 3. The composition for external application for skin according to claim 2, wherein the moringa seed is germinated moringa seed. The composition for external application for skin according to claim 1 or 2, wherein the extract is contained in an amount of 0.01 to 95% by weight based on the total weight of the composition. The external preparation composition for skin according to claim 1 or 2, wherein the composition is for skin whitening. The skin of claim 1 or 2, wherein the composition further contains at least one whitening substance selected from the group consisting of ascorbyl glucoside, licorice extract, arbutin, ascorbic acid, kojic acid and niacinamide. External preparation composition. The external preparation composition for skin according to claim 6, wherein the whitening substance is contained in an amount of 0.5 to 5.0% by weight based on the total weight of the composition.