KR20120139005A - Natural cosmetic composition, skin sheet and storage container comprising the same - Google Patents

Abstract

The present invention relates to a natural cosmetic composition and a sheet for skin using the same, and more particularly, a skin conditioning agent, a metal ion sequestrant, a moisturizer, a thickener, a preservative, a solubilizer, and a skin conditioning agent, which are made of a natural substance extract and a natural substance powder. By including, pH adjuster, antioxidants, fragrances, alcohol and water, more active substances of natural substances can be absorbed into the skin than natural extracts are used alone. It can give you confidence.

Description

Natural Cosmetic Composition, Skin Sheet And Storage Container Comprising The Same}

The present invention relates to a natural cosmetic composition comprising a skin conditioning agent made of a natural substance, a skin container and a storage container including a natural cosmetic composition.

The skin is divided into three layers of epidermis, dermis, and subcutaneous fat tissue in order from the outside, and is a very important tissue that not only protects the human body while directly contacting the external environment but also has biochemical and physical functions.

The skin is covered with sebum, sweat, dust, and dead skin cells.In summer, when the outside temperature is high, or during puberty, when the hormone is secreted, excess sebum can cause acne or seborrheic dermatitis. There is a possibility of being damaged by exposure to ultraviolet rays due to contamination and destruction of the ozone layer. Therefore, the selection of functional products related to cosmetics or cosmetics used directly on the skin has emerged as an important issue in terms of protecting the skin with concern about side effects.

These cosmetics should be free of side effects, human-friendly, and safe to use on specific constitutions, atopic skin or sensitive skin. The plant natural materials are not only excellent in safety, but also have various components beneficial to the human body, and thus are preferred as ingredients that provide moisturizing, whitening, and anti-aging effects.

Therefore, conventionally, there have been efforts to find extracts that have excellent safety and provide various functionalities from herbals, vegetables, fruits, flowers, etc., which have a function of suppressing skin aging and whitening.

Korean Patent Laid-Open Publication No. 2003-64059 discloses an anti-aging cosmetic composition containing an extract of bark and a silk tree extract. The above technique has antioxidative activity, collagen fiber biosynthesis effect and skin elasticity enhancing effect by using a mixture of bark extract and silk tree extract, but the amount of the active substance lost by high heat and solvent increases as trees are manufactured as extract. A large amount of wood is required and the user may not be able to identify whether the cosmetics containing the extract of the trees may have questions about this.

In order to overcome this, Korean Patent Laid-Open Publication No. 2008-33668 describes a gel type mask containing natural plant flakes. The above technique is to crush the lyophilized plants into small flakes, and then to include them in a gel-type mask, which has more active substances than the same amount of the extracts, and the flakes are visually confirmed. Some of the natural plant flakes contained in the gel coated with a certain thickness on the release paper film does not touch the skin there is a problem that the active substance is not delivered to the skin.

An object of the present invention is to provide a natural cosmetic composition comprising a skin conditioning agent consisting of a natural substance extract and a natural substance powder.

Another object of the present invention is to provide a skin sheet impregnated with a natural cosmetic composition.

In addition, another object of the present invention is to provide a storage container containing a natural cosmetic composition and a nonwoven fabric.

In order to achieve the above object, the natural cosmetic composition of the present invention comprises a skin conditioning agent and an additive consisting of a natural substance extract and a natural substance powder.

The additives are metal ion sequestrants, humectants, thickeners, preservatives, solubilizers, skin conditioning agents, pH adjusters, antioxidants, fragrances, alcohols and water.

The natural cosmetic composition is 0.05 to 5% by weight of skin conditioning agent, 0.01 to 0.1% by weight of metal ion sequestrant, 5 to 15% by weight moisturizer, 0.01 to 2% by weight thickener, 0.1 to 1% by weight preservative, solubilizer 0.05 to 0.5 weight percent, 0.05-0.5 weight percent pH adjuster, 0.05-0.5 weight percent antioxidant, 0.01-0.5 weight percent fragrance, 1-5 weight percent alcohol and 73-85 weight percent water.

The natural substance extract and natural substance powder is mixed in a weight ratio of 1: 1 to 10.

The natural substance is in the group consisting of apricot, acerola, aloe vera, pineapple, banana, cherry, lemon, strawberry, raspberry, pomegranate, kiwi, citrus, mango, orange, grape, papaya, peach, pear, apple, tomato and citron It is one or more selected.

The natural material powder is pulverized so that the particle size of the freeze-dried natural material is 0.3 to 1.7 mm.

The lyophilized natural material is lyophilized for 22 to 26 hours at -75 to -65 ℃ and 800 to 900 m torr.

In addition, the skin sheet of the present invention is impregnated with a natural cosmetic composition in order to achieve the above object.

In addition, in order to achieve the above another object, the storage container of the present invention is formed of a transparent vinyl divided into two sides, one side contains a nonwoven fabric to be impregnated with a cosmetic composition, and the other contains a natural cosmetic composition.

The natural cosmetic composition of the present invention includes a large amount of the active substance of the natural substance by including a skin conditioning agent consisting of a natural substance extract and a natural substance powder, and the active substance is directly impregnated into the nonwoven fabric so that the active substance is directly delivered to the skin. do. Therefore, a large amount of active substances are absorbed into the skin, resulting in excellent moisturizing, anti-aging, freckles removal, whitening, exfoliation, nutrition, anti-inflammatory, antibacterial, skin elasticity, collagen production, wrinkle suppression and sebum secretion control. .

In addition, the present invention can increase the reliability of the product because the user can check whether the natural substance is added to the naked eye using the natural substance powder.

In addition, the present invention can be used in any way desired by the user by providing a container containing a non-woven fabric on one side and a natural cosmetic composition on the other side.

1 is a view showing a storage container containing a non-woven fabric on one side and a natural cosmetic composition on the other side according to an embodiment of the present invention,
2 is a view showing a method for impregnating a non-woven fabric with a natural cosmetic composition according to an embodiment of the present invention.

The present invention comprises a skin conditioning agent, a metal ion blocker, a moisturizer, a thickener, a preservative, a solubilizer, a skin conditioner, a pH adjuster, an antioxidant, a fragrance, alcohol and water composed of natural extracts and natural powders. It is related to a natural cosmetic composition and a skin sheet using the same, which can give a greater confidence to consumers because the natural substance active substance can be absorbed into the skin more than when using the substance extract alone, and the natural substance powder is visually identified.

Hereinafter, the present invention will be described in detail.

The present invention is impregnated with a nonwoven fabric a natural cosmetic composition including skin conditioner, metal ion blocker, moisturizer, thickener, preservative, solubilizer, skin conditioner, pH adjuster, antioxidant, fragrance, alcohol and water Can be used.

The skin conditioning agent has the functions of moisturizing, anti-aging, blemish freckles, whitening, exfoliation, nutrition, anti-inflammatory, antibacterial, skin elasticity, collagen production, anti-wrinkle and sebum secretion control, specifically natural substances It is to use the mixture of the extract and natural powder in a weight ratio of 1: 1 to 10.

If the mixing ratio is less than 1: 1, the amount of the active substance absorbed into the skin is small as the content of the natural substance powder containing a large amount of the active substance is small, so various functions may not be exhibited. In the case of more than 10, a large amount of natural substance powder may stick to the user's skin and cause inconvenience to the user.

Natural substance extracts used as skin conditioning agents are prepared according to conventional methods.

The natural material powder is prepared by lyophilizing the natural material for 22 to 26 hours at a temperature of -75 to -65 ℃ and a vacuum of 800 to 900 m torr and then grinding it to a particle size of 0.3 to 1.7 mm.

If the natural material is not lyophilized at a temperature of -75 to -65 ℃ and a vacuum of 800 to 900 m torr for 22 to 26 hours, a large amount of the active ingredient of the natural material is lost, and it is difficult to store for a long time and processed in its own state It can be difficult to do.

In addition, if the particle size of the natural material powder is less than 0.3 mm, the skin sheet is used after the use of the fine natural material powder may cause discomfort to the user, if the particle size exceeds 1.7 mm, impregnating the skin sheet For example, when storing the cosmetic composition, the powder may be aggregated to one side by the weight of the natural powder.

 Natural substances used as natural extracts and natural powders include apricot, acerola, aloe vera, pineapple, banana, cherry, lemon, strawberry, raspberry, pomegranate, kiwi, citrus, mango, orange, grape, papaya, peach, pear, At least one selected from the group consisting of apples, tomatoes and citron.

The content of the skin conditioning agent is 0.05 to 5% by weight, preferably 0.1 to 3% by weight. When the content of the skin conditioner is less than 0.05% by weight, various functions of natural substances may not be exhibited. When the content of the skin conditioner is more than 5% by weight, products of various properties cannot be manufactured and light or external Discoloration or odor may occur by contact.

The metal ion blocking agent is to remove iron and copper ions affecting the rancidity of the cosmetic composition, specifically, one or two of disodium ethylenediamine tetraacetic acid (NTA) and nitrilotriacetic acid (NTA) Can be mentioned.

The content of the metal ion blocking agent is 0.01 to 0.1% by weight, preferably 0.01 to 0.05% by weight. When the content of the metal ion blocking agent is less than 0.01% by weight, the metal component may react with the raw material to reduce the stability of the cosmetic composition, and when the content is more than 0.1% by weight, it may cause skin irritation or allergy.

The moisturizing agent supplies moisture to the skin, specifically, glycerin, butylene glycol, dipropylene glycol, ethylene glycol, mineral oil, iso 1 type, or 2 or more types chosen from the group which consists of propyl myristate, dimethicone, and petrolatum.

The content of the humectant is 5 to 15% by weight, preferably 8 to 13% by weight. If the content of the moisturizing agent is less than 5% by weight may reduce the moisturizing function, if the content is more than 15% by weight may cause discomfort to the user due to the sticky nature of the moisturizer.

The thickener is to improve the viscosity of the cosmetic composition, specifically hydroxyethyl acrylate (hydroxyethyl acrylate), sodium acryloyl dimethyl taurate copolymer (piso-150 pentaerythritol tetra) 1 type selected from the group consisting of stearate (PEG-150 Pentaerythrityl Tetrastearate), Carbomer, Hycel, Xanthan Gum, Microcrystalline Wax and Beeswax Or 2 or more types can be mentioned.

The content of the thickener is from 0.01 to 2% by weight, preferably from 0.01 to 1.5% by weight. If the content of the thickener is less than 0.01% by weight, the viscosity may be lowered and the cosmetic composition may flow out of the skin sheet.If the content of the thickener is more than 2% by weight, the viscosity of the cosmetic composition may be increased to give stickiness during use. If it is above a certain viscosity, the essence may not be fully impregnated with the nonwoven fabric.

The preservative prevents rancidity of the cosmetic composition, specifically, imidazolidinyl urea (Imidazolidinyl Urea), methylparaben (Methylparaben), salicylic acid (Salicylic acid), methylchloroisothiazolinone (Methylchloroisothiazolinone), methyl isodia Group consisting of Methylisothiazolinone, Ethylparaben, Butylparaben, Propylparaben, Isopropylparaben, Phenoxyethanol and DMDM Hydantoin The 1 type or 2 or more types chosen from are mentioned.

The content of the preservative is 0.1 to 1% by weight, preferably 0.1 to 0.5% by weight. If the content of the preservative is less than 0.1% by weight, microbial preservatives may be weak, which may affect the formulation stability. If the content is more than 1% by weight, trouble may occur on the skin.

The solubilizer facilitates mixing of the additives and water, and facilitates the penetration of the skin conditioning agent into the skin. Specifically, PG-60 Hydrogenated Castor Oil and three The 1 type or 2 types selected from the tetraaryl alcohol (Cetearyl Alcohol) can be mentioned.

The content of solubilizer is 0.05 to 0.5% by weight, preferably 0.1 to 0.5% by weight. When the content of the solubilizer is less than 0.05% by weight, the additive and water may not be mixed, and when the content is more than 0.5% by weight, trouble may occur on the skin.

The pH adjusting agent is to have a pH value of 5.0 to 7.0 of the cosmetic composition, specifically, potassium hydroxide (Potassium Hydroxide) or triethanolamine (Triethanolamine) is used.

The content of the pH adjuster is 0.05 to 0.5% by weight, preferably 0.1 to 0.3% by weight. When the content of the pH adjuster is less than 0.05% by weight, the skin may be stingy, and when the content is more than 0.5% by weight, skin irritation may occur beyond the pH of the skin, 7.0.

The antioxidant is to stabilize the cosmetic composition and prevent the oxidation of the skin conditioning agent, specifically, tocopheryl acetate, tocopheryl succinate, tocopherol (tocophero), vitamin E, vitamin C, as 1 or 2 or more selected from the group consisting of ascorbyl palmitate and L-ascorbate sodium.

The content of antioxidant is 0.05 to 0.5% by weight, preferably 0.1 to 0.3% by weight. When the content of the antioxidant is less than 0.05% by weight, the cosmetic composition may be easily rancid. When the content is more than 0.5% by weight, the stability of the cosmetic composition may be lowered.

The alcohol sterilizes the skin, and specifically, one or two or more selected from the group consisting of ethanol, methanol, benzyl alcohol and isopropyl alcohol.

The content of alcohol is 1 to 5% by weight, preferably 3 to 4% by weight. If the content of alcohol is less than 1% by weight can not exhibit a bactericidal effect, if the content is more than 5% by weight may cause problems on the skin.

The fragrance is to impart a fragrance to the cosmetic composition, the content of the fragrance is 0.01 to 0.5% by weight. When the content of the fragrance is less than 0.01% by weight, only the fragrance of the skin conditioning agent may be generated slightly, and when the content is more than 0.5% by weight, the fragrance may be too strong.

The water is preferably used at 73 to 85% by weight.

The viscosity of the cosmetic composition of the present invention is from 800 to 2000 cSt at 20 ℃. If the viscosity is less than 800 cSt, the cosmetic composition may flow out of the skin sheet and the natural powder may settle. If the viscosity is more than 2000 cSt, it may be inconvenient to use because it is sticky.

The skin conditioning agent of the present invention is not only used for the composition used for the skin sheet, but may be used for cleansing cream, massage cream, nutrition cream, cleansing gel, massage gel, skin and lotion.

In addition, the present invention provides a storage container containing a natural cosmetic composition and a nonwoven fabric as shown in FIG.

The storage container is formed of transparent vinyl divided into two sides, with nonwoven fabric on one side and natural cosmetic composition on the other.

The method of using the cosmetic composition and the nonwoven fabric contained in the storage container is, for example, by impregnating the cosmetic composition into the nonwoven fabric by putting the cosmetic composition contained in one side of the storage container into the nonwoven fabric contained in the other side as shown in FIG. A nonwoven fabric, such as a skin sheet, impregnated with a cosmetic composition is used on the skin.

In another example, a nonwoven fabric is added to a cosmetic composition contained on one side of the storage container to impregnate the cosmetic composition with a nonwoven fabric, and then the skin sheet thus prepared is used on the skin.

The method of impregnating a cosmetic composition and a nonwoven fabric is not limited to this, Any method may be sufficient as it is a method of impregnating a cosmetic composition and a nonwoven fabric.

The nonwoven fabric impregnated with the natural cosmetic composition is called a skin sheet, and the skin sheet means all sheets used for the skin such as the face, neck, and the back of the hand.

In addition, other methods of use may be used in a variety of ways, such as applying the cosmetic composition on the skin without first impregnating the cosmetic composition and the nonwoven fabric, then raising the nonwoven fabric, putting the nonwoven fabric on the skin first, and then applying the cosmetic composition. .

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Production Example 1 Manufacture of natural substance (apricot) powder

Apricots are placed in a lyophilizer (PVTFD 100R, Ilshin Lab) and pre-frozen and lyophilized for 24 hours at a temperature of -70 ° C and a vacuum of 900 m torr. Lyophilized apricots were ground with a grinder (AM-7, Hyronchem) to obtain a particle size of 0.5 to 0.8 mm to prepare a natural powder.

Production Example 2 Manufacture of natural (mango) powder

Mangoes are placed in a lyophilizer (PVTFD 100R, Ilshin Lab) and pre-frozen and lyophilized for 24 hours at a temperature of −70 ° C. and a vacuum of 900 m torr. Lyophilized mango was pulverized with a pulverizer to obtain a particle size of 0.6 to 1.0 mm to produce a natural powder.

Preparation Example 3. Manufacture of natural substance (apricot) extract

After extracting the apricots with an extract solvent with 70% ethanol for 3 days at room temperature with an extractor, the extract was filtered with a 400 mesh filter cloth to prepare a natural extract.

Preparation Example 4. Manufacture of natural substance (strawberry) extract

Methanol was used as an extractant to extract strawberries at room temperature for 2 days, and then the extract was filtered through a 400 mesh filter cloth to prepare a natural substance extract.

Example 1.

To 81.99% by weight of water, 0.01% by weight of disodium ethane as a metal ion blocking agent, 5% by weight of glycerin as a moisturizer, 3% by weight of butylene glycol, 2% by weight of dipropylene glycol, 0.02% by weight of xanthan gum as a thickener, and hydroxyethyl 0.5% by weight of acrylate, 0.5% by weight of sodium acryloyldimethyltaurate copolymer, 0.08% by weight of carbomer, 0.18% by weight of methylparaben as preservative, 0.02% by weight of propylparaben, 0.1% by weight of phenoxyethanol, as a solubilizer 0.2% by weight of -60 hydrogenated castor oil, 0.1% by weight of tocopheryl acetate as an antioxidant, and mixed at 30 ° C. for 30 minutes, followed by 2% by weight of skin conditioner, 0.1% by weight of perfume, and 4% by weight of ethanol with alcohol. Add and stir for 10 minutes. Thereafter, 0.2 wt% of triethanolamine was added as a pH adjusting agent to obtain a natural cosmetic composition having a pH value of 6.5 and a viscosity of 1100 cSt at 20 ° C.

The skin conditioning agent is a mixture of apricot powder prepared in Preparation Example 1 and the apricot extract prepared in Preparation Example 3 in a weight ratio of 5: 1.

Example 2.

The same procedure as in Example 1 was carried out, but the mango powder prepared in Preparation Example 2 and the strawberry extract prepared in Preparation Example 4 were mixed at a weight ratio of 7: 1 to obtain a natural cosmetic composition as a skin conditioning agent.

Comparative Example 1

To 50 g of the pulverized, 1000 g of a mixed solution of methanol, propylene glycol and distilled water mixed in a weight ratio of 2: 1: 1 were added, and the resultant was extracted at 75 ° C. for 5 hours. The prepared extract was filtered with a 200 mesh filter cloth (Cheongggye Sangpo) and filtered with a filter No. 2 filter at room temperature to remove insoluble matters. The extract was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor and then lyophilized to obtain 7.5 g of dry weight extract.

In addition, a weight of 8.2 g of the Albizia julibrissin extract was obtained by the same method as the preparation method of the extract of bark skin.

The soybean bark extract and the extract of Albizia julibrissin were mixed in a weight ratio of 1: 0.5 using a stirrer.

76.13 wt% water, 10 wt% of the above mixed extract, 0.15 wt% carbomer, 0.02 wt% disodium ethane, 1 wt% butylene glycol, 0.2 wt% methylparaffin, and 0.1 wt% propylparaben 2% by weight of Macadamianet O'Neill, 6% by weight of mineral oil, 2% by weight of dimethicone, 0.4% by weight of sorbitassesquioleate and 1.6% by weight of polysorbate 60 as oil. Put and mix. To the mixture was neutralized by addition of 0.15% by weight of triethanol amine, followed by cooling to 45 ° C, followed by addition of 0.25% by weight of perfume, and cooling to 35 ° C to prepare a nourishing lotion.

Comparative Example 2

After dispersing 3% agar and 1% sodium alginate in an appropriate amount of purified water, the solution is sufficiently dissolved by heating at 60 ° C. Then, 6% of sodium acrylate is slowly mixed to maintain the temperature at about 55 ° C. Then, 0.1% of aluminum hydroxide and 0.005% of calcium chloride were dispersed and mixed for about 2 hours, and the composition was uniformly dispersed by containing 0.5% of flake powder having a particle size of 2 mm.

Comparative Example 3

In the same manner as in Example 1, it was prepared using 85.03% by weight of water without using a skin conditioning agent. At this time, the pH value of the cosmetic composition is 7.0, the viscosity is 600 cSt at 20 ℃.

Test example.

1. Moisturizing power measurement: Each cosmetic composition was titrated on different plates, and then left in a desiccator adjusted to a relative humidity of 50% and a temperature of 35 ° C. using completely dried calcium chloride (CaCl ₂ ) as an absorbent. And the weight of each cosmetic composition was measured four times. Moisturizing effect was measured using the weight change of the plate, it was calculated using the following equation (1).

In Equation 1, 'S' means the weight of the cosmetic composition used, 'ΔS' means the amount of weight change of the cosmetic composition.

2. Determination of antioxidant effect: 500 ml of the cosmetic composition was diluted in 1 ml of 0.1 mM DPPH solution dissolved in methanol, mixed and mixed for 10 minutes at room temperature, and absorbance was measured at 565 nm. This is to measure the DPPH radical scavenging effect of the cosmetic composition using the reducibility of DPPH (1,1-diphenyl-2-picrylhydrazyl), and purified water was used as a control instead of the cosmetic composition.

3. Measurement of the whitening effect: The cosmetic composition was prepared by diluting the cosmetic composition with a final concentration of 50 ㎍ / ml by adding 20 µl of the cosmetic composition using 0.1 M sodium phosphate buffer (pH 6.5), and then preparing a 96-well plate (well plate) 100 μl of 1.5 mM tyrosine (in 1 N HCl), 20 μl of the diluted cosmetic composition, 3 wells per concentration and 80 μl of 100 U / ml tyrosinase. After mixing it was reacted for 15 minutes in a 37 ℃ incubator (incubator). After the reaction, the absorbance value was measured at 490 nm with an ELISA reader, and purified water was used instead of the cosmetic composition as a control. This is to measure the whitening effect of the cosmetic composition by evaluating the relative enzyme activity by measuring the amount of dopachrome produced by tyrosinase using a substrate by absorbance.

4. Measurement of cell damage protection capacity: After inoculation of human-derived keratinocytes (HaCaT cells) to the bottom of the culture dish, penicillin (100 U / ㎖), streptomycin (100 ㎍ / ㎖) and 10% FBS (Fetal Bovin) Dulbecco's Modified Eagle's Medium (DMEM) medium containing Serum) was added and cultured in an incubator containing 5% carbon dioxide at 37 ° C. The cultured HaCaT cells were diluted in a concentration of 1 × 10 ⁵ cells / ml in 96-well plates and inoculated with 100 μl and incubated for 24 hours. After incubation, all medium was removed, and each cosmetic composition was diluted to a final concentration of 10, 50, and 100 ㎍ / ml using a medium that did not contain serum, and then treated 100 μl per well three times, and then added for 24 hours. Incubated with The cultured cells were treated with 200 μM of hydrogen peroxide (H ₂ O ₂ ) as a cell damaging material, and cultured in an incubator containing 5% carbon dioxide at 37 ° C. for 4 hours. After 4 hours, the medium was removed and fresh serum-free medium was inoculated in 100 μl and 20 μl of MTT reagent dissolved at 5 mg / ml using PBS and incubated for 4 hours. After removing all of the medium containing the MTT reagent and the sample, 100 μl of isopropanol was added to each well and stirred for 15 to 20 minutes. Then, the absorbance value was measured at 570 nm using an ELISA reader, and purified water was used as a control.

Cell damage protection was measured by the following method.

5. Determination of anti-inflammatory effect: Penicillin (100 U / mL), streptomycin (100 μg / mL) and 10% Fetal bovine serum (FBS) after inoculation of rat-derived macrophages (Raw264.7) at the bottom of the culture dish. DMEM (Dulbecco's modified Eagle's medium) containing the medium was incubated in an incubator containing 5% carbon dioxide at 37 ℃. The cultured Raw264.7 cells were seeded in 96-well plates at a concentration of ⁵ × 10 ⁵ cells / ml and incubated for 24 hours. After incubation, the medium was removed, and the cosmetic composition was diluted to a final concentration of 50 μg / ml using a medium containing serum (LPS 100 ng / ml), and then added to each well, followed by further incubation for 24 hours. The medium was recovered, centrifuged at 12,000 rpm for 10 minutes at 4 ° C., and only 50 μl of the medium was placed in a 96-well plate and reacted with the same amount of Griss reagent for 10 minutes. After the reaction, the absorbance value was measured at 540 nm using an ELISA reader, and purified water was used as a control. This is because excessive production of NO (Nitric Oxide), a reactive nitric species (RNS), induces an inflammatory response. For this, the cells are stimulated with lipopoly-saccharide (LPS) to induce the production of NO, followed by the production of NO by the cosmetic composition. Inhibitory effect is measured by 'Griess reagent' method.

6. Wrinkle inhibitory effect measurement: After inoculating human-derived fibroblasts (CCD-986sk) to the bottom of the culture dish, penicillin (100 U / ㎖), streptomycin (100 ㎍ / ㎖) and 10% FBS (fetal bovine serum) ) Was added and cultured in an incubator containing 5% carbon dioxide at 37 ° C. The cultured CCD-986sk cells were diluted to a concentration of 1 × 10 ⁵ cells / ml in a 96-well plate and inoculated with 100 μl and incubated for 24 hours. After incubation, all of the medium was removed and 90 μl of the medium without serum was added to each well. The cosmetic composition was treated in the well so that the final concentration of 50 ㎍ / ㎖ 10 ㎕ added. After incubation for 24 hours, 20 μl of MTT reagent dissolved in 5 mg / ml was added using PBS and incubated for 4 hours. Remove all medium containing MTT reagent and sample, add 100 µl of isopropanol (acid isopropanol; 0.04N HCl in iso-propanol) to each well, stir for 30 minutes, and use an ELISA reader at 570 nm. Absorbance values were measured and purified water was used as a control.

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Moisturizing power (%) Early 100 100 100 100 100 15 hours later 71.3 72.3 54.3 69.3 65.9 19 hours later 64.1 65.2 49.1 58.0 53.6 24 hours later 55.4 54.1 38.0 50.9 47.1 Antioxidant (IC ₅₀ (μg / ml)) 32 29 94 156.1 141.1 Whitening
(Tyrosinase inhibitory activity) (%) density:
50 μg / ml 3.11 2.84 30.6 29.1 16.2 Cell damage protection (%) density:
10 μg / ml 32.05 34.11 4.36 6.23 4.12 density:
50 μg / ml 32.13 34.76 6.89 6.59 6.39 density:
100 μg / ml 32.29 35.08 6.74 7.02 6.99 Anti-inflammatory
(NO generation inhibitory ability) (%) density:
50 μg / ml 24.3 25.9 6.8 4.3 3.9 Anti-wrinkle
(Cell proliferation) (%) density:
50 μg / ml 34.5 36.9 22.9 12.1 16.2

As shown in Table 1, the cosmetic compositions of Examples 1 and 2 of the present invention was confirmed that the moisturizing, antioxidant effect, whitening effect, cell damage protection effect, anti-inflammatory effect and anti-wrinkle effect is excellent.

On the other hand, the cosmetic composition of Comparative Examples 1 to 3 is not excellent in moisturizing, antioxidant effect, whitening effect, cell damage protection effect, anti-inflammatory effect and anti-wrinkle effect compared to the cosmetic composition of Examples 1 and 2.

Claims (10)

Natural cosmetic composition comprising a skin conditioning agent and additive consisting of a natural substance extract and a natural substance powder.
The natural cosmetic composition of claim 1, wherein the additive comprises a metal ion sequestrant, a moisturizer, a thickener, a preservative, a solubilizer, a skin conditioner, a pH adjuster, an antioxidant, a perfume, an alcohol, and water.
According to claim 2, wherein the natural cosmetic composition is 0.05 to 5% by weight skin conditioner, 0.01 to 0.1% by weight of metal ion blocker, 5 to 15% by weight moisturizer, 0.01 to 2% by weight thickener, 0.1 to 1% by weight preservative %, Solubilizer 0.05-0.5 wt%, pH adjuster 0.05-0.5 wt%, antioxidant 0.05-0.5 wt%, fragrance 0.01-0.5 wt%, alcohol 1-5 wt% and water 73-85 wt% Cosmetic composition.
The natural cosmetic composition of claim 1, wherein the natural substance extract and the natural substance powder are mixed in a weight ratio of 1: 1 to 10.
According to claim 1, The natural substance is apricot, acerola, aloe vera, pineapple, banana, cherry, lemon, strawberry, raspberry, pomegranate, kiwi, citrus, mango, orange, grape, papaya, peach, pear, apple, tomato And at least one natural cosmetic composition selected from the group consisting of citron.
The natural cosmetic composition according to claim 1, wherein the natural powder is ground to have a particle size of 0.3 to 1.7 mm of the lyophilized natural material.
The natural cosmetic composition of claim 6, wherein the lyophilized natural material is lyophilized for 22 to 26 hours at a temperature of −75 to −65 ° C. and a vacuum of 800 to 900 m torr.
The natural cosmetic composition of claim 1, wherein the natural cosmetic composition has a viscosity of 800 to 2000 cSt at 20 ℃.
A skin sheet impregnated with a natural cosmetic composition according to any one of claims 1 to 8.
A storage container formed of transparent vinyl divided into two sides, the non-woven fabric on one side and the natural cosmetic composition according to any one of claims 1 to 8 on the other side.

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