KR20120056978A - Solution filter container for extracting cells - Google Patents

Solution filter container for extracting cells Download PDF

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Publication number
KR20120056978A
KR20120056978A KR1020100118493A KR20100118493A KR20120056978A KR 20120056978 A KR20120056978 A KR 20120056978A KR 1020100118493 A KR1020100118493 A KR 1020100118493A KR 20100118493 A KR20100118493 A KR 20100118493A KR 20120056978 A KR20120056978 A KR 20120056978A
Authority
KR
South Korea
Prior art keywords
solution
filter
chamber
opening means
valve mechanism
Prior art date
Application number
KR1020100118493A
Other languages
Korean (ko)
Inventor
김홍곤
Original Assignee
(주)대홍메디칼
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)대홍메디칼 filed Critical (주)대홍메디칼
Priority to KR1020100118493A priority Critical patent/KR20120056978A/en
Publication of KR20120056978A publication Critical patent/KR20120056978A/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14—Details; Accessories therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures

Abstract

PURPOSE: A solution filter container is provided to reduce costs for components and to remarkably reduce a specimen cell extracting time. CONSTITUTION: A solution filter container comprises a body(100), a filter member(200), and an opening member. The body comprises an upper chamber, a lower chamber, and a valve device. The upper chamber comprises an inner space housing solution including cells and the lower chamber is arranged in the lower part of the upper chamber. The valve device is arranged between the upper and lower chambers, thereby supplying or secluding the solution flowing into the lower chamber. The filter member is joined to the lower chamber of the body.

Description

SOLUTION FILTER CONTAINER FOR EXTRACTING CELLS}

The present invention relates to a solution filter container, in particular, having a solution container integrally formed with a filter to simplify the parts consumed in the extraction operation of the sample cells to reduce the cost, and to significantly reduce the extraction time The solution filter vessel is improved so that its structure.

In general, to check for abnormalities in the body, cells corresponding to the physical examination site are collected, and the collected cells are preserved in a solution container containing a cell preservation solution, and the cells are attached to a slide and then examined under a microscope. Doing.

Conventionally, there is a separate solution container for dispersing the cells attached to the brush in the liquid, shake the solution container and pour the liquid into another container while the cells are sufficiently spread in the liquid and inhale the liquid by closely attaching the suction device under the filter. In addition, the filter is filtered by rubbing the cells filtered on the slide.

When the container and the body are separated to bring the sampling membrane to the slide, the mixed liquid left in the container is poured down by gravity and disappeared.

However, the process of moving the cells from the solution container to the slides is time-consuming because it must be done manually by the user, and contaminants or impurities can be transferred from the user's hand to the slides, thereby reducing the accuracy of the cell test. there was.

In addition, foreign matter may be contained in addition to the cells collected in the solution container, in which case it is not easy to separate the foreign matter and cells, a separate tool for separation is required.

SUMMARY OF THE INVENTION An object of the present invention is to provide a solution filter container having an improved structure to provide a filter-integrated solution container, thereby reducing costs and easily separating the solution solution from the sample cells.

The present invention for achieving the above object has an upper space of the inner space having an interior space for accommodating the solution solution containing the cells, the upper chamber and the lower chamber disposed below the upper chamber, and between the upper and lower chambers A container body disposed with and provided with a valve mechanism for supplying / blocking a solution solution flowing into the lower chamber;

A filter member coupled to a lower chamber of the container body and having a filter disposed at an upper portion thereof;

And an opening means disposed at an upper portion of the upper chamber to open the valve mechanism in a pressing operation.

The valve mechanism is disposed between the upper and lower chambers, the passage member having a fluid passage;

The valve member is fitted into the fluid passage of the passage member and is moved downward by the opening means to open the fluid passage.

The opening means is a cap member coupled to the outer peripheral surface of the upper chamber;

It is formed to extend in the center lower portion of the cap member and the lowering operation by the pressing operation is provided with a push member for transmitting the lowering force to the valve mechanism side.

The opening means is a central portion of the push member is punched and the perforated inner circumferential surface and the upper outer circumferential surface of the push member are integrally connected via an elastic piece having an elastic restoring force.

The push member has a hollow portion in which the upper and lower communication.

The valve mechanism is disposed between the upper and lower chambers, the passage member having a fluid passage;

It is provided with a valve member of the material fitted into the fluid passage of the passage member and punctured by the pressing operation of the opening means.

The opening means may include a cap member coupled to the outer circumferential surface of the upper chamber;

It is formed in the central lower portion of the cap member and the lowering operation by the pressing operation is provided with a perforated member having a lower end portion is in the form of a wedge to form a cutting hole in the lower portion is a path through which the solution solution flows.

The present invention is composed of a filter-integrated solution container to reduce the cost of parts and significantly reduce the extraction time of the sample cell, according to the present invention is provided with a solution container integrated with the filter sample cell Since the parts consumed in the extraction operation can be simplified, the cost can be reduced, and the extraction operation time can be greatly reduced.

1 is a perspective view showing one embodiment of a solution filter container according to the present invention.
2 is an exploded perspective view of FIG.
Figure 3 is a state of use showing the state of use of the present invention opening means and valve mechanism.
Figure 4 is a cross-sectional view showing another example of the push member of the present invention.
5 is a perspective view showing another embodiment of a solution filter container according to the present invention.
6 is a front view of FIG. 5;
Figure 7 is a cross-sectional view showing the punching member of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

An embodiment of the solution filter container according to the present invention will be described with reference to FIGS. 1 to 3, the configuration of which has an inner space in which a solution solution containing sample cells is contained, and an upper chamber 110 having an upper opening. A container body 100 having a lower chamber 120 disposed below the upper chamber 110 and separated by a valve mechanism for supplying / closing a solution solution; A filter member 200 coupled to the lower chamber 120 of the container body 100 and having a filter 210 disposed thereon; It is disposed on the upper chamber 110, the opening means for opening the valve mechanism in the pressing operation.

In more detail, the valve mechanism is disposed between the upper and lower chambers 110 and 120 and has a fluid passage, and a passage member 130, which is fitted into the fluid passage of the passage member 130 and is opened by the opening means. It is composed of a valve member 135 for lowering operation to open the fluid passage.

The valve member 135 is deformed by the lower pressing force of the opening means, and when the lower pressing force is removed, is composed of an elastic body so as to be restored to its original form. For example, a silicon material may be employed.

The passage member 130 has a structure formed to extend from the lower bottom surface of the upper chamber 110 to the interior of the lower chamber 120, and is formed in a narrower shape toward the lower side for the inlet of the lower chamber 120 of the solution solution. Has a structure.

The opening means is formed in the cap member 310 coupled to the outer circumferential surface of the upper chamber 110 and extends in the lower portion of the center of the cap member 310 and is lowered by a pressing operation to transfer the lowering force to the valve mechanism side. It consists of a push member (320).

The cap member 310 has a structure in which the edge portion is fastened by screwing the outer circumferential surface of the upper chamber 110 and the center portion is perforated.

The push member 320 is connected to the upper portion integrally with the perforated central portion of the cap member 310 via a plurality of elastic pieces 315. This is to give a restoring force to return again after the push member 320 is lowered.

The lower end of the push member 320 is preferably arranged to be in contact with the upper surface of the valve member 135 at all times. This is to quickly transmit the pressing force to the valve member 135 when the force pushing the push member 320 downward.

An inlet hole 326 that is a flow path of the solution solution is formed below the push member 320.

The sealing member 330 may be further interposed between the cap member 310 and the upper chamber 110.

The sealing member 330 seals the upper portion of the upper chamber 110 to block external leakage of the solution solution.

The filter member 200 is inserted such that the upper portion is accommodated in the lower chamber 120.

Preferably, strip-shaped protrusions 205 and 125 are formed on the outer circumferential surfaces of the filter member 200 and the lower chamber 120 to prevent slippage when the clamping mechanism is coupled.

In addition, the filter member 200 has a plurality of through-holes 202 are formed in communication with the top and bottom, the filter 210 having a filter film function is disposed above the through-holes 202.

More preferably, the cushioning material 220 of the compressed sponge material is preferably disposed between the filter 210 and the through hole 202 to provide a cushioning function when the slide and the filter 210 contact each other.

Referring to the operation of the embodiment of the present invention having such a configuration as follows.

In one embodiment of the solution filter container according to the present invention, after the cap member 310 and the sealing member 330 in which the push member 320 is integrated are separated from the container body 100 to open the upper chamber 110. The solution solution containing the sample cells is introduced into the upper chamber 110 of the container body 100.

At this time, the filter member 200 maintains the state coupled to the lower chamber 120 of the container body (100).

Thereafter, the cap member 310 and the sealing member 330 are closed on the container body 100 again to seal the upper chamber 110 of the container body 100, and then the push member 320 is pressed downward, and the push member 320 is closed. ) Transmits the downward pressing force to the valve member 135, the valve member 135 is contracted and deformed.

At this time, the lowering operation of the push member 320, since one end of the elastic piece 315 for supporting the push member 320 is connected to the inner surface of the cap member 310, the other end of the elastic piece 315 is lower While extending to guide the lowering operation of the push member (320).

As the valve member 135 contracts and deforms, the fluid passage of the passage member 130 is opened, and the solution solution contained in the upper chamber 110 is lower chamber 120 through the inlet hole 326 of the push member 320. Flows into the filter member 200.

Subsequently, the sample cells contained in the solution solution are attached to the upper surface of the filter 210 of the filter member 200, and the remaining solution solution is drained downward through the through hole 202 of the filter member 200.

That is, the solution solution and the sample cells contained in the upper chamber 110 flows into the lower chamber 120 side as the valve member 135 of the valve mechanism opens and closes the fluid passage of the passage member 130. While passing only the solution solution while passing through the filter 210 of the filter member 200, the sample cell has a filtering process that is seated on top of the filter 210.

Thereafter, after separating the filter member 210 from the lower chamber 120, the sample cells seated on the upper side of the filter 210 is brought into contact with a slide (not shown) to collect the sample cells.

On the other hand, when the pressing force is pushed downward to the push member 320, as the elastic piece 315 is retracted to the original position, the push member 320 is raised to return to the original position.

Thereafter, the downward pressing force transmitted to the valve member 135 is removed, and the valve member 135 is restored to its original form by self-resilience to seal the fluid passage.

As a result, the solution solution remaining in the upper chamber 110 does not flow into the lower chamber 120 and remains as the valve mechanism is sealed.

This is applicable when it is necessary to divide and test a sample cell several times.

4 is a view showing another example of an embodiment of the present invention, in which the hollow part 325 with the upper and lower openings formed in the push member 320 of the previous embodiment is formed.

When the new solution solution is supplied to the lower part of the upper chamber 110 through the hollow part 325 of the push member 320, the existing solution solution containing the sample cells is floated, and the new solution solution is valve member 135. Through the filter 210 is first introduced into the filter 210 side.

This prevents the drying of sample cells attached to the filter 210 as the new and clean solution solution wets the filter 210 through the hollow part 325, and the existing solution contained in the upper chamber 110. The function of diluting the solution may be performed.

At this time, a stopper for blocking external foreign substances from entering the hollow portion 325 of the push member 320 may be coupled.

5 to 7 is a view showing another embodiment of the present invention, the configuration has an opening means different from the previous embodiment, the other components are the same, so duplicate description will be omitted.

Specific configuration of another embodiment of the present invention is connected to the cap member 310, which is an element constituting the opening means via the elastic piece 315, the lower end is formed in a wedge shape so as to puncture the valve member 135, The lower end is provided with a perforation member 350 is formed with a cutting hole 356 is a flow path of the solution solution.

The perforated member 350 may be formed in the hollow 355 to be an inflow path of a new solution solution therein as in the previous embodiment.

In another embodiment of the present invention having such a configuration, the filtering process of the filter member 200 is the same, but the method of opening the valve mechanism by puncturing the valve member 135.

That is, when the punching member 350 is pushed downward, as the elastic piece 315 is extended and the lower end of the punching member 350 penetrates the valve member 135, the solution solution of the upper chamber 110 is drilled. It is introduced into the lower chamber 120 through the incision hole 356 of the 350.

Afterwards, the solution solution introduced into the lower chamber 120 is filtered through the filter member 200, and thus the description thereof will not be repeated.

One embodiment and the other embodiment of the present invention, but the valve mechanism is different from each other, but after the sample cells contained in the solution solution flows into the container body 100 consisting of the upper and lower chambers 110 and 120, the upper and lower After opening the valve mechanism that separates the failure chambers 110 and 120, the same filtering process is performed to extract the sample cells contained in the solution solution.

Therefore, the present invention has a solution container integrally formed with the filter member, thereby simplifying the parts consumed in the extraction work of the sample cells, thereby reducing the cost and significantly reducing the extraction time. Has

Description of the Related Art [0002]
100: container body 110: upper chamber
120: lower chamber 130: passage member
135 valve member 200 filter member
202: through hole 210: filter
310: cap member 315: elastic piece
320: push member 325: hollow part
326: inlet hole 330: sealing material
350: punched member 355: hollow
356: incision hole

Claims (7)

A solution solution having an internal space in which a solution solution containing cells is accommodated and having an upper opening, a lower chamber disposed below the upper chamber, and a lower chamber disposed between the upper chamber and the lower chamber, and flowing into the lower chamber. A container body provided with a valve mechanism for supplying / blocking the oil;
A filter member coupled to a lower chamber of the container body and having a filter disposed at an upper portion thereof;
And an opening means disposed above the upper chamber to open the valve mechanism in a pushing operation. The method according to claim 1,
The valve mechanism is disposed between the upper and lower chambers, the passage member having a fluid passage;
And a valve member fitted into the fluid passage of the passage member and lowered by the opening means to open the fluid passage. The method according to claim 1 or 2,
The opening means is a cap member coupled to the outer peripheral surface of the upper chamber;
And a push member extending downwardly from the center portion of the cap member and being pushed down by a pressing operation to transmit a lowering force to the valve mechanism side. The method according to claim 3,
The opening means is a solution filter container, characterized in that the central portion of the push member is perforated and the perforated inner circumferential surface and the upper outer circumferential surface of the push member are integrally connected via an elastic piece having elastic restoring force. The method according to claim 3,
The push member is a solution filter container, characterized in that the hollow portion is formed in communication with the top and bottom. The method according to claim 1,
The valve mechanism is disposed between the upper and lower chambers, the passage member having a fluid passage;
And a valve member of a material which is fitted into the fluid passage of the passage member and is perforated by the pressing operation of the opening means. The method of claim 6,
The opening means may include a cap member coupled to the outer circumferential surface of the upper chamber;
It is formed in the lower portion of the center of the cap member is lowered by the pressing operation to the lower end portion having a wedge shape to puncture the valve member and the lower portion is provided with a perforated member formed in the cut hole which is a path through which the solution solution flows Solution filter vessel.
KR1020100118493A 2010-11-26 2010-11-26 Solution filter container for extracting cells KR20120056978A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020100118493A KR20120056978A (en) 2010-11-26 2010-11-26 Solution filter container for extracting cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020100118493A KR20120056978A (en) 2010-11-26 2010-11-26 Solution filter container for extracting cells

Publications (1)

Publication Number Publication Date
KR20120056978A true KR20120056978A (en) 2012-06-05

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KR1020100118493A KR20120056978A (en) 2010-11-26 2010-11-26 Solution filter container for extracting cells

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101390920B1 (en) * 2013-12-13 2014-04-30 김준우 The device for fat disrupting and the element separator which has the function of fat disrupting
CN107907520A (en) * 2017-12-12 2018-04-13 宁波博奥生物工程有限公司 One kind separation preserves reagent filtering detection device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101390920B1 (en) * 2013-12-13 2014-04-30 김준우 The device for fat disrupting and the element separator which has the function of fat disrupting
WO2015088194A1 (en) * 2013-12-13 2015-06-18 김준우 Fat crusher and component separator allowing fat crushing
CN107907520A (en) * 2017-12-12 2018-04-13 宁波博奥生物工程有限公司 One kind separation preserves reagent filtering detection device
CN107907520B (en) * 2017-12-12 2024-02-27 宁波博奥生物工程有限公司 Separation preservation reagent filters detection device

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