KR20120026260A - Method of manufacturing moisture absorbent and deodorant by microorganism on diatomite and opal - Google Patents

Abstract

PURPOSE: A microorganism deodorizing and moisture absorbing agent using opal and diatomite is provided to offer excellent deodorization and moisture removal effects to users, and to use the agent instead of charcoal. CONSTITUTION: A microorganism deodorizing and moisture absorbing agent is obtained by the following steps: crushing opal and diatomite, and mixing 45-75wt% of opal in the particle size of 100-300 meshes, and 25-55wt% of crushed diatomite; stirring 90wt% of mixture with 10wt% of water, and molding the mixture into granules having the diameter of 1-5mm using a granule making machine or a pellet making machine; and drying the obtained granules using a hot air dryer for 4-6 hours or using sunlight for 4-8 hours two times.

Description

TECHNICAL OF MANUFACTURING MOISTURE ABSORBENT AND DEODORANT BY MICROORGANISM ON DIATOMITE AND OPAL}

Eco-friendly yet excellent deodorizing, deodorizing effect, and dehumidification by applying physical and biochemical methods by inoculating and cultivating useful microorganisms by making granular composition using the excellent adsorption principle using the pore and diatomite of pore in natural ore. The present invention relates to a multifunctional deodorant dehumidifying agent having a hygroscopic function, and more particularly, to processing opal and diatomaceous earth having various mixed micropores of fine nanoscale size to micron scale by the method described in the present invention, and further improving the adsorption power. Deodorizing and dehumidifying agents that act on microorganisms such as Pseudomonas sp., Which can decompose and remove sulfur compounds, and Thiobacillus sp., Which can decompose and remove hydrogen sulfide, and Nitrosomonas sp., Which can remove ammonia. In the industrial and everyday life sectors Be able to be used as a substitute for a salt, silica gel desiccant in use today, and further environmental pollution and the method of producing a large amount of the available as a substitute for the carbon in the char to discharge friendly deodorant desiccant composition in the manufacture

Republic of Korea Patent Publication No. 2005-0093048 is made of natural silicate composition by mixing ocher, illite, zeolite, diatomaceous earth 1: 1: 1: 1, the other composition is 60% ~ 90% natural stone, tourmaline 20% ~ 5%, 10% ~ 1% anion stone, 5% ~ 10% charcoal, 0.1% ~ 5% of photocatalyst titanium dioxide (TiO2) is added, and the functional mixture and natural silicate composition are again 1: 1 ~ 2: 1 It proposes a plastering composition to improve the indoor air quality to be mixed by mixing.

Korean Patent Laid-Open Publication No. 2006-0059110 relates to a building finishing material having a function of reducing heating and cooling costs, reducing fine dust, cement toxicity, and toxic neutralization of a binder, using natural mineral powder ceramics as a main raw material. Elite) 325-400 mesh, antimicrobial, deodorant, mold, etc. mixed with powder ceramics and liquid natural flavor liquid binders containing water-soluble emulsion, and installed on the walls, ceilings and floors of the building fresh air It is mentioned that it can provide a pleasant indoor environment by supplying it.

The composition of the proposed patent mentions that it is possible to improve the quality of indoor air mainly by including natural minerals.

Therefore, the present inventors can maximize the effect of reducing deodorization and dehumidification function at the same time, cost-effective and environmentally friendly, harmless to the human body when manufacturing a deodorant, dehumidifying agent using microorganisms that act only on harmful gases as well as natural minerals. It was found that the present invention was completed.

Natural Chloride Composition for Blocking Harmful Substances Using Natural Minerals and Vegetable Materials (ex. Patent Publication No. 2005-0093048) Manufacturing method of functional building finish material for improving indoor air quality (ex. Patent Publication No. 2006-0059110) Odor remover using zeolite and rice bran (e.g. Patent Publication No. 90-256) Deodorant based on microbial strain and iron sulfate (e.g., patent publication 93-002264) Deodorants using polyhydric alcohols and chlorites (e.g., Patent Publication 94-003569) Chitosan-phthalocyanine-based deodorant (e.g., patent publication 97-061275) Deodorant using hypochlorous acid and yeast (e.g., patent publication 98-085090)

[Reference 1] The Korean Journal of Industrial Hygiene Vol. 18, No. 1 (2008. 3) pp.80-90 1226-4326 Listed in KCI [Ref. 2] Snow Calcium Chloride Kills Tree by / Road Management Team, Infrastructure & Transportation Division, Ministry of Construction and Transportation [on-line], February 2006. (Search date: August 27, 2010)

In modern life, environmental pollution is getting more serious, and various indoor and outdoor air pollutants are suffering from direct and indirect damage.

In particular, the damage of sick house syndrome and new car syndrome caused by harmful compounds that are invisible from indoor interior products and finishes is more serious.

Deodorants or dehumidifiers are used to remove or reduce these pollutants. Conventional deodorants, dehumidifiers, and fragrances are mostly used by chemically forming or using charcoal to process harmful or unfriendly materials.

 Some deodorants with advanced porous structures made of zeolite and bentonite minerals are used.

However, chemically formed deodorant and dehumidifying agents are mainly calcium chloride or silica gel. Calcium chloride is the same material used as dehumidifying and deicing agent. It is a kind of chemical chemical formula of CaCl2, which generates heat in the process of absorbing solid snow powder as liquid. When calcium chloride is sprayed on the snow, it dissolves and absorbs moisture around it.

It is a principle that absorbs water vapor floating in the air and collects it in a plastic container. In other words, the underwater submarines gather together to become water. Because it is similar to salt water, the water collected in the dehumidifier made of calcium chloride is not the main cause of environmental pollution, but when it is thrown away, it can be corroded to metal by simply throwing it into the sewer. It may be thrown away.

Contact with calcium chloride can cause the skin to become red or crusty temporarily (and calcium chloride can cause damage to the stomach when taken directly into the body). It is an irritating powder, which is used by workers in departments that pack calcium chloride. Symptoms of irritation of the mucous membranes are reported, and may cause skin irritation.

In the case of silica gel used as a dehumidifying agent for food or industrial products, silica gel is harmless in the normal process, but most of the manufacturing plants are in China, and more than 99% of the products distributed in Korea are made in China. Most of the silica gel produced in China contains a chemical called DMF (Dimethyl Fumerate) called DMF, a toxic substance.

DMF is mainly added to the desiccant "silica gel," which is placed in the box with the product to prevent the occurrence of mold, which may occur during transportation or storage, for example. Pain during skin contact, itching, inflammation, breathing on inhalation It is a chemical that can cause disorders.

In accordance with the new Regulation (Commission Decision 2009/251 / EC), which came into effect on 1 May this year, the European Union prohibits the manufacture and import into Europe if the DMF content is more than 0.1 ppm. Ordered.

Dehumidifiers and deodorants that are mainly composed of these chemical components are harmful to the human body and cause environmental pollution.

And dehumidification and deodorant made of charcoal can also be used for a short period of time because of the pore of the surface blocked by contaminants, and there are many problems such as carbon emission rights problem that is a problem worldwide by generating a large amount of carbon dioxide during the first manufacturing. .

Some deodorants with advanced porous structures made of zeolite and bentonite-based inorganic materials are used, but in the case of zeolites, excessively fine and uniform size pores interfere with the adsorption of organic compounds of various structures. There is a problem, and bentonite-based inorganic materials also have similar problems. In general, the hygroscopic agent is widely used to protect the product from moisture in the packaging of the product throughout the industry, and several types of hygroscopic agents have been provided.

Some of the moisture absorbents that are typically used are made of pure calcium chloride mineral as raw material in addition to silica gel, but these moisture absorbents have the following disadvantages.

In other words, silica gel is produced by decomposing sodium silicate with strong acid, so it is expensive to manufacture and does not rot when used products are disposed of. There was a problem with acidification contamination.

In addition, the solution produced after absorbing pure calcium chloride product may cause fatal problems with metals or other products. Also, products using some minerals are formed by granulating powdered powder. The water could ooze out of the packaging, making it undesirable for packaging food, pharmaceuticals, and semiconductor components.

In addition, natural diatomaceous earth is impregnated with calcium chloride or magnesium chloride solution, but chlorine group (Cl), which is determined to be harmful from calcium chloride or magnesium chloride, is released. It reacted and rusted, so it was not suitable for packaging semiconductor parts.

The present invention uses diatomaceous earth as the main raw material and other porous minerals as a subsidiary material, and properly mixes various pores of various sizes of micropores and macropores, that is, nano-to-micron-sized pore sizes. Its purpose is to provide a deodorizing and dehumidifying agent that can exert deodorizing and dehumidifying function with strong adsorption effect, which can be produced eco-friendly and harmless to human beings and economical cost necessary for daily life by doubling the effect of adsorbing. .

Pseudomonas sp., Which can process opal and diatomaceous earth having various and mixed micropores of fine nanoscale to micron size by the method described in the present invention and can decompose and remove sulfur compounds to have more improved adsorptive power. And microorganisms such as nitrosomonas sp., Which can remove ammonia, and thiobacillus sp., Which can decompose and remove hydrogen sulfide.

Deodorant dehumidifying agent having both odor decomposition and hygroscopic function at the same time 45-75% by weight of opalite, diatomaceous earth granule material to prepare a composition that acts as a carrier of useful microorganisms to act both physical and biological solutions simultaneously 30% by weight of the mixture, 10% by weight of water and 90% by weight of the mixture kneading in the granulation form of the first step of the molding step, and to make the composition to remove and remove harmful substances of microorganisms Characterized in that the microbial inoculation culture is divided into two stages of manufacture

The present invention can be expected to be sufficient as a deodorization function and dehumidification function using physical adsorption effect by ion exchange between the pores of natural ore protein and diatomaceous earth, but also sulfur compounds, Pseudomonas can be decomposed and removed. By using microorganisms such as Pseudomonas sp. And Thiobacillus sp., Which can decompose and remove hydrogen sulfide, and Nitrosomonas sp., Which can remove ammonia, biological adsorption effects can be expected at the same time. Excellent deodorant dehumidification effect can be expected.

When used in a confined space such as a refrigerator or a closet shoe cabinet, it can absorb harmful compounds and absorb moisture to reduce damage from harmful compounds in the air such as sick house syndrome and new car syndrome.It absorbs moisture in the air and maintains an appropriate humidity condition. It can reduce the damage of virus from the humidity and prevent the cold and lead to a comfortable indoor space life.

Representative diagram is a schematic diagram of a deodorant dehumidifying agent in the form of a granule-type microbial carrier according to an embodiment of the present invention.
The arrow indicated by 1 in the diagram shows the composition made according to the contents of claim 1 after inoculating microorganisms such as Pseudomonas sp. And Thiobacillus sp. Nitrosomonas sp. It describes the state of microorganisms attached by using as a carrier.
2, which is indicated by the arrows in the contents of the representative figure, describes various pore forms of the composition made according to the contents of claim 1, and describes nanopores having a nano size (100 nm).
3, which is indicated by the arrows in the contents of the representative figure, describes various pore types of the composition made according to the contents of claim 1, and describes micron-sized (μm) -sized macropores (Hole).
4, which is indicated by the arrows in the representation, illustrates the surface of the composition made according to the contents of claim 1.

In order to complete the present invention, the mixing step of opal 45-75% by weight, diatomaceous earth granular material 25-30% by weight, the dough 10% by weight of the mixture 90% by weight of the molding step to form a granular composition 1 In step 2, the microbial treatment of inoculating and incubating the microorganisms capable of decomposing and removing harmful compounds of the microorganisms in the prepared composition is prepared in two stages.

The main ingredient of opal, which is the main raw material to be used in the present invention, is a hard-leaf cancer protein, which is a phosphate or radioactive ore with an SiO2 content of 90% or more, and is gray in color, and contains 90% or more of natural silicic acid due to its chemical composition. Good quality siliceous.

This process is repeated many times as the opalite erupts from the Cretaceous volcanic eruption and spews high-temperature volcanic powder and falls into the freshwater lake as glass beads. Volcanic powder sediment is integrated with microorganisms and is transformed into volcanic rock ore due to the change of the earth, and it is characterized by pores of various sizes with nano- and micro-level pores of layered and fibrous structure naturally occurring rather than artificial processing.

Table 1 below shows the major chemical components analyzed by Korea Chemical Testing Institute.

ingredient SiO₂ AI₂O₃ Fe₂O₃ MgO CaO Na₂O K₂O TiO₂ LOS content 90.18 2.48 1.03 0.14 0.82 0.15 0.48 0.08 3.98

As can be seen from Table 1, it is a high purity natural silica material containing more than 90% by weight of SiO2. The content of SiO2 is more than 20 ~ 30% more than that of conventional zeolite and illite.

In addition, diatomaceous earth to be used as an auxiliary material is a sediment in which the harmfulness of a single cell algae called diatoms (Diatoms-cell wall is composed of SiO2) is deposited on the sea floor (seawater) or porridge (freshwater). It is a kind of fossil soil in which these strata are formed.

The diatomaceous earth is mixed with clay, sand, volcanic ash, vegetative corrosive, iron hydroxide, iron sulfide, and the like, when they are formed, and also contains a lot of water.

Diatomaceous earth contains a variety of contaminants, the most common of which are organic matter, sand, and iron oxide. The organic substance can be removed by calcination, and the sand can be removed by iron chloride, and the iron oxide can be removed by iron chloride by mixing the salt with calcination at 700 ~ 800 ℃.

 Fossil diatoms are about the size of a micron and can be magnified hundreds of times with a microscope

Only good and clear diatomaceous earth is very light, soft, and porous enough to absorb up to five times the volume of liquid, due to the large number of pores.

The structure is a collection of porous cells such as circular, needle and semicircular.

By using this opalite as the main raw material and using diatomaceous earth as an auxiliary raw material, it is possible to double the adsorption capacity of volatile organic compounds such as VOCS by converting various pore sizes of various sizes of micropores and macropores, that is, nanopore to micron pore size. I can make it.

The composition method to be used in the present invention is to create a good environment for living microorganisms by forming a single-phase multi-porous inorganic raw protein and diatomaceous earth in a certain step as described below, and can be expected to have sufficient deodorization and dehumidification effect even without inoculation culture of microorganisms. However, in the second step, it is intended to complete the present invention to obtain a more stable and efficient deodorizing and dehumidifying effect by adding a biological microbial culture inoculation process.

Stage 1:

(a) Grinding step using a particle size of 100 ~ 300 mesh (mesh) by grinding the opal and diatomaceous earth.

(b) 45-75% by weight of opalite, 25-55% by weight of diatomaceous earth granular material in the mixing step.

(c) 10% by weight of water as a diluent; (b) agitation at a rate of 90% by weight of the mixture.

(d) molding step using a pallet or granulator to form granules having a diameter of 1 to 5 mm.

(e) a drying step of drying the molded granules once every 4 to 6 hours using a heat dryer or removing moisture twice a day for 4 to 8 hours under natural light.

At this time, the moisture content is dried below 1% to remove moisture as much as possible.

Step 2:

The microorganisms to be used are microorganism strains such as Pseudomonas sp., Which can decompose and remove sulfur compounds, and Nitrosomonas sp., Which can remove ammonia and Thiobacillus sp., Which can decompose and remove hydrogen sulfide. do.

One or more strains of microorganisms with excellent degrading activity in organic components were inoculated into the primary liquid medium to obtain 25? After incubation for 24 hours at 30 ℃ using the composition prepared in step 1 as a growth substrate and preservative carrier of the microorganism strain 30? Secondary solid incubation for 48 hours at 37 ℃ to produce a seed.

At this time, as a strain of microorganisms having excellent organic component decomposition activity even at medium and high temperatures (30 to 45 ℃), nitrozo which can remove Pseudomonas sp. And Thiobacillus sp. Ammonia which can decompose and remove hydrogen sulfide Most preferred is Moss (Nitrosomonas sp.) And the like. These strains can be used in combination of 2 or 3 or more types.

In addition, Pseudomonas sp: Thiobacillus sp: nitrozomos 2: 2: 1 to the produced spawn is mixed by ventilation drying at room temperature for 4 hours to finally prepare a microbial preparation.

As described above, microorganisms with high growth rate and excellent decomposition activity for sulfur compounds, hydrogen sulfide, ammonia, etc. are used as spawns, causing problems in aeration tanks (various operating factors, complexity of processing process, excessive operating cost, and treatment efficiency decrease). Can overcome.

0.1 g of the sample was suspended in 9.9 ml of physiological saline (NaCl 0.85%), diluted 105-fold and plated in a one-step composition, and then incubated at 30 ° C. for 48 hours. The total number of bacteria after incubation was about 3.5 × 10 9 CFU / g.

1. Decompose and remove microorganisms of sulfur compounds

Metabolic process: H2S → SO42- + Energy + Acid

* Applicable microorganism: Pseudomonas sp. Etc

2. Hydrogen Sulfide Removal Microorganism

* Applicable microorganism: Thiobacillus sp. Etc

3. Ammonia Removal Microorganism

Metabolic process: NH3 → NO2- + Energy + Acid

* Applicable microorganism: Nitrosomonas sp. Etc

Pseudomonas sp.

-As a genus of the true fungus Pseudomonas family inhabit in soil, fresh water, seawater. A genus containing as many as 150 species, in which cells move with short hairs or short hairs, are nonmotile bacillus, often fluorescent or give pigments such as green, blue, purple, yellow, and insoluble. Some may have a bright red or yellow pigment.

-Gram-negative bacteria, widely distributed in soil, fresh water and seawater.

-Aerobic but denitrifying or nitric acid breathing also grows anaerobic and does not fix nitrogen.

-Can decompose and use a wide range of organic substances such as aliphatic hydrocarbons, phenols, terpenes and steroids.

P. aeruginosa, P. fluorescens, etc.

Strains include P. cepacia. P. putida, P. fluorescens.

-It is a rod-like bacillus with flagella at the end of the cell, and has a high rooting rate.

-Pseudomonas bacteria are produced by sideropore (Fe transporter), which is combined with iron on the root surface to prevent harmful bacteria. Many strains can grow at low temperatures.

-Dissolve pathogen cell wall with chitinase secretion and inhibit pathogen with secretion of antibiotics (pyrrolnitrin, pyoluteorin, phenazines).

-Suppressing vegetables, seedlings and wilts. High activity against filamentous fungi. Suppresses spinach wilting, tomato wilting, vegetable mozzarella.

Thiobacillus sp.

It is an independent nutrient bacterium that uses inorganic carbon compounds such as carbon dioxide as a carbon source. It is a sulfated bacterium that can be odorized in the form of sulfuric acid, which is a final metabolite using hydrogen sulfide as an energy source.

Hydrogen sulfide, which is a major odor source caused by the decay of organic matter, efficiently at room temperature and atmospheric pressure

Can be effectively removed, and ammonia gas

It is inexpensive and can easily deal with odor generated from various waste water.

Nitrosomonas sp.

First genus of nitrite bacteria. Ellipsoidal or monobacterium with 1 to 2 subpolar flagella. It is a gram-negative, chemically independent nutrient that oxidizes ammonia to nitrous acid and simultaneously fixes carbonic acid. It is a combination aerobic bacteria and widely distributed in freshwater, ocean and soil.

Test Example 1) Deodorizing Force Test

The deodorant completed by the manufacturing process of the present invention was commissioned by the Korea Institute of Far-Infrared Application Evaluation, and the deodorizing effect was measured. The test method was performed by injecting 500 ppm of ammonia gas into a container containing a deodorant and a dehumidifying agent sample and a container containing no sample. KFIA-FI-1004 test standard for checking the deodorization rate was used.

As a result, as shown in the deodorization rate (%) test results of Table 2 and the gas concentration curve according to the time elapse of FIG. 3, the gas concentration was measured every 30 minutes using a gas detector tube. The deodorization rate was 93% after the first 30 minutes, the deodorization rate was 96% at 60 minutes, the deodorization rate was 97% at 90 minutes, and the deodorization rate was 99% at 120 minutes. Most of the ammonia gas was adsorbed and removed, and the deodorization efficiency was very high.

Deodorization test Test Items Elapsed time (minutes) Blank (ppm) Sample concentration (ppm) Deodorization rate (%)

Deodorization test
Early 500 500 - 30 490 35 93 60 480 20 96 90 460 15 97 120 450 5 99

Test Example 2 Measurement of Antibacterial Effect (Measuring Reduction of Bacteria)

The deodorant and dehumidifying agent of the present invention was commissioned by the Korea Far Infrared Application Evaluation Institute, and the antimicrobial effect was measured. In the test method, E. coli and Pseudomonas aeruginosa were inoculated into four containers for 18 hours, respectively, and two containers were added with a deodorant sample. KFIA-FI-1002 test standard was used to measure the percentage reduction of bacteriostatic bacteria after 24 hours.

As a result of the bacterium reduction rate (%) test result of Table 3, the bacterium concentration was measured after 24 hours, and the bacterium reduction rate was 82% in the container in which E. coli cultured with the deodorant sample was incubated. In the old container, the bacteriostatic rate was 80%, which was very high.

In addition, it was shown that the reduction rate (%) of bacterium did not change in the container into which the sample was not injected.

Test bacterium reduction rate (%) Test Items Sample classification Initial concentration Concentration after 24 hours Bacteriostatic reduction (%) Antibacterial test by E. coli Blank 6
2.5 x 10
6
6.2 x 10 - Deodorant, Dehumidifier 5
4.5 x 10 82.0 Antibacterial test by E. coli
Blank 7
2.7 x 10 7
6.9 x 10 - Deodorant, Dehumidifier 6
5.4 x 10 80.0

(Note) 1) Blank: Measured without sample.

2) The number of bacteria on the medium is calculated by multiplying the dilution factor. End.

1) Test Method: KFIA-FI-1002

2) Use strain

Escherichia coil atcc 25922

Pseudomonas aeruginosa ATCC 15442

Although the preferred embodiments of the present invention have been illustrated and described above, the present invention is not limited to the above-described embodiments, and the present invention is not limited to the above-described embodiments without departing from the spirit of the present invention as claimed in the claims. Various modifications can be made by those skilled in the art, and such modifications are intended to fall within the scope of the appended claims.

Claims (3)

Grind the opal and diatomaceous earth, and use a particle size of 100 ~ 300 mesh to grind the mixture evenly at the ratio of 45-75 wt% of opal stone and 25-55 wt% of diatomite granular material. (b) the mixture is subjected to a stirring step at a ratio of 90% by weight to form an amorphous granule having a diameter of 1 to 5 mm using a pallet or a granulator, and then the formed granules using a heat dryer. A deodorant dehumidifying agent that has been dried once every 6 hours or dried twice a day for 4-8 hours under natural light. Microorganisms such as Pseudomonas sp., Which decomposes and removes sulfur compounds, and Nitrosomonas sp., Which can remove ammonia, Thiobacillus sp., Which can decompose and remove hydrogen sulfide. To deodorize the source of odor by The deodorant and dehumidifying agent according to claim 1 may be used in the form of a 1-5mm flat or spherical shape, and includes both a flat or spherical shape.

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