KR20070049065A - Bioreactor - Google Patents

Abstract

(assignment)

It provides a bioreactor capable of high gaseous liquid solid separation efficiency and thus high bioreaction efficiency in a vessel.

(Solution)

The bioreaction zone S in which sludge floats from the vicinity of the middle in the vessel 1 to the bottom is suspended. The raw water is subjected to anaerobic biotreatment in this bioreaction zone S, and then flows into the gas liquid solid separator 4 in the upper part of the container 1 to separate the gas liquid solids. Gas liquid solid The treated water is discharged out of the container 1 through the treated water discharge pipe 6. The solids settle into the gas liquid solid separator 4 and are returned to the bioreaction zone S in the vessel 1 through the solids discharge pipe 7. In the gas liquid solid separator 4, baffles 21 to 24 made of an annular plate member having an inclined form in which the diameter becomes smaller toward the lower side are provided. Each of the baffles 21 to 24 has the same center and is provided at regular intervals from each other.

Description

Bioreactor {BIOREACTOR}

1 is a longitudinal sectional view of a bioreactor according to an embodiment.

FIG. 2 is a diagram showing a gas liquid solid separator on the upper part of the bioreactor, (a) is an enlarged view of the upper part of the bioreactor of FIG. 1, and (b) is a plan view of the gas liquid solid separator. to be.

3 is a longitudinal sectional view of a baffle.

4 is a view showing a gas liquid solid separator on the upper part of the bioreactor according to another embodiment, (a) is an enlarged view of the upper part of the bioreactor, and (b) is a perspective view of the gas liquid solid separator.

5 is a plan view showing another embodiment.

6 is another plan view showing another embodiment.

7 is a longitudinal sectional view of a bioreactor according to another embodiment.

* Explanation of symbols on the main parts of the drawings *

1, 1A container 4, 4A, 4D gas liquid solid separator

7, 7D solids discharge pipe 7a upper pipe

7b Lower pipe 10 Cylindrical section

10a Gas removal port 11 Conical section

13, 13A inlet 14 jet nozzle

17 Degassing hole 21 to 24 baffles

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to bioreactors suitable for biological treatment of wastewater, and more particularly, to a bioreactor in which a gas liquid solid separation chamber is provided in an upper portion of a vessel for bioreaction.

Bioprocessing apparatuses of Upflow Anaerobic Sludge Blanket (ESA) or Expanded Granular Sludge Bed (EVS) type are widely used for the biological treatment of organic wastewater. According to these UASB, EGSB process, organic substances in the waste water are converted to methane and CO ₂ gas by anaerobic (嫌氣性) biological treatment. For this reason, the biologically treated water includes gas (methane, CO ₂ ), liquid (water) and solids (sludge). In order to efficiently perform the NAS and EBS processing, it is necessary to separate gas liquid solids so that solids do not flow out.

Japanese Patent Application Laid-Open Nos. 10-165980 and 7-507233 disclose sedimentation and separation of solids on the upper part of a biological treatment reactor such as a BAS method. It is described to install a gas liquid solid separator for separation.

Patent Document 1: Japanese Patent Application Laid-Open No. 10-165980

Patent Document 2: Publication No. 7-507233

The gas liquid solid separator used in Japanese Patent Application Laid-Open Nos. 10-165980 and 7-507233 has a simple structure and low gas liquid solid separation efficiency.

An object of the present invention is to provide a bioreactor having a high gas liquid solids separation efficiency and thus improving the bioreaction efficiency in a container.

The bioreactor of claim 1 is provided with a vessel in which a bioreaction treatment of a liquid is performed inside, and a liquid is introduced into the vessel and liquid is introduced from the vessel, so that the sedimentation separation of solids in the liquid and the flotation separation of gas are performed. In the bioreactor having a gas liquid solid separation chamber comprising a plurality of baffles having an inclined shape that is installed at regular intervals with the same center in the gas liquid solid separation chamber, the diameter becomes smaller toward the bottom It is characterized by being installed.

The bioreactor of claim 2, wherein the gas liquid solid separation chamber is provided with an upper cylindrical portion and a conical portion continuous to the lower side of the cylindrical portion. Solid discharge pipe for flowing out the solid precipitated downward in the gas liquid solid separation chamber by gravity from the gas liquid solid separation chamber extending downward from the lower end of the conical portion, the vertical length of the solid discharge pipe h ₂ is 0.1 times or more of the inner diameter d ₁ of the cylindrical portion.

The bioreactor of claim 3, wherein the solid discharge pipe is inclined with respect to the vertical direction and extends, and the opening at the lower end of the solid discharge pipe is in a horizontal direction or obliquely upward. .

The bioreactor according to claim 4, wherein the gas in the gas liquid solid separation chamber or the solid discharge tube is placed in the gas liquid solid separation chamber or in the lower part of the gas liquid solid separation chamber or the upper part of the solid discharge pipe. And a gas removal hole for flowing out of the solid discharge pipe.

The bioreactor of claim 5, wherein the solid content discharge pipe is composed of an upper pipe which is installed to extend in a vertical direction, and a lower pipe which is connected to the upper pipe and inclined in a horizontal direction or downward, The opening of the distal end of the lower tube is characterized in that the direction inclined in the transverse direction or upward.

The bioreactor of claim 6 is characterized in that a gas removal hole is provided in the lower part of the upper pipe or the lower pipe to allow gas in the solid discharge pipe to flow out of the solid discharge pipe.

The bioreactor of claim 7, wherein an upper end of the outermost circumferential baffle is continuous with an inner surface of the gas liquid solid separation chamber, and the gas liquid solid separation chamber according to claim 1. Between the inner surface of the baffle and the outermost circumference, the inlet is formed so that the liquid in the vessel flows into the gas liquid solid separation chamber in a substantially tangential direction (接線 方向).

The bioreactor of claim 8, wherein the gas removal port for flowing gas outward from the inner surface of the gas liquid solid separation chamber and the outermost circumference baffle is formed in the cylindrical portion. It is characterized by.

(Example)

EMBODIMENT OF THE INVENTION Hereinafter, an Example is described with reference to drawings.

(Bioreactor with slanted entire solids discharge pipe)

Figure 1 is a longitudinal cross-sectional view of the bioreactor according to the embodiment, Figure 2 (a) is an enlarged view of the top of the bioreactor of Figure 1, Figure 2 (b) is a plan view of the gas liquid solid separator, Figure 3 is a longitudinal cross-sectional view of the baffle. . 2 (a) shows a cross section along the line A-A in FIG. 2 (b). Figure 4 (a) is an enlarged view of the top of the bioreactor according to another embodiment, Figure 4 (b) is a perspective view of the gas liquid solid separator of Figure 4 (a).

In the embodiment shown in Figs. 1 to 3, the container 1 is cylindrical, and raw water is supplied from the raw water pipe 2 to the distributor 3 in the lower part of the container 1, and the container 1 is supplied through the distributor 3. Raw water flows into the bottom of the container.

It is a bioreaction zone S in which sludge floats from the middle of the vessel 1 or slightly above the top to the bottom. After undergoing anaerobic biotreatment in the bioreaction zone S, the raw water is introduced into the gas liquid solid separator 4 (gas liquid solid separation chamber) in the upper part of the container 1 and separated into gas liquid solids.

In addition, most of the gases escape from the water surface in the container 1 without entering the gas liquid solid separator 4 and are discharged to the outside of the container 1 through the gas removal port 5 of the top of the container 1. Some of the gas is introduced into the gas liquid solid separator 4, but is separated from the water surface in the container 1 and discharged to the outside of the gas liquid solid separator 4 or from the water surface of the gas liquid solid separator 4 through the gas removal port 10a described later. Is discharged through the gas removal port 5. The treated liquid separated from the gas liquid solid separator 4 is discharged to the outside of the container 1 through the treated water discharge pipe 6. The solids settle into the gas liquid solid separator 4 and are returned to the bioreaction zone S in the vessel 1 through the solids discharge pipe 7.

The configuration of the gas liquid solid separator 4 will be described with reference to FIGS. 2 and 3.

The outer appearance of the gas liquid solid separator 4 is composed of a cylindrical portion 10 of the upper portion and a conical portion 11 having a slanted shape which is connected to the lower side of the cylindrical portion 10 and directed downward. The solid discharge pipe 7 is connected to the lower end of this conical part 11. In the present embodiment, the entire pipe of the solid discharge pipe 7 is inclined, but the solid discharge pipe 7 may be extended in the vertical direction and may be installed at an inclined vicinity of the lower end of the discharge pipe 7. Moreover, although the opening of the lower end of the said discharge pipe 7 is obliquely upwards, it may be a horizontal direction, and angle (theta) ₃ is 0-20 degrees, and especially 10-15 degrees are preferable.

The trough 12 which flows in the process water in the upper part of the cylindrical part 10 is provided. The treated water discharge pipe 6 (omitted in the drawing in FIGS. 2 to 4) is connected to the cylindrical portion 10 so as to discharge the treated water flowing over the trough 12.

Inlet 13 is formed in the vicinity of the lower portion of the cylindrical portion 10 to introduce the bioreaction water into the gas liquid solid separator 4. In the embodiment of Fig. 2, the outer surface of the cylindrical portion 10 is provided with a guide 13a for guiding the biological reaction water so that the biological reaction water flows tangentially into the cylindrical portion 10 through the inlet 13. The inlet 13 and the guide 13a constitute the inlet of the bioreaction water. In this embodiment, the inlet linear velocity of the bioreaction water from the inlet 13 is 2 to 8 cm / s, in particular 4 to 6 cm. It is preferable that it is / s.

In the lower portion of the conical portion 11, a jet nozzle 14 is provided for ejecting water toward the solid discharge tube 7 so that the lower portion of the conical portion 11 or the solid discharge tube 7 can be cleaned. The jet nozzle 14 is capable of supplying high pressure water from a pump (not shown in the drawing) via a pipe 15. However, this jet nozzle 14 may be omitted.

In this embodiment, a gas pocket 16 for collecting gas is formed in the upper part of the solid discharge pipe 7, and a gas removal hole 17 for discharging gas from the gas pocket 16 to the outside of the discharge pipe 7 is formed. However, the gas pocket 16 and the gas removal hole 17 may be omitted.

In the cylindrical part 10, the baffles 21-24 which consist of the annular plate material of the inclined form which become small in diameter downward are provided. Each of the baffles 21 to 24 has the same center and is provided at regular intervals from each other. In this embodiment, a total of four baffles are provided from the innermost baffle 21 to the outermost baffle 24, but the number of baffles is not limited to four.

The upper end of the outermost circumference baffle 24 is in contact with or fixed to the inner circumferential surface of the cylindrical portion 10. The inlet is open toward the outermost circumference baffle 24 and the inner peripheral surface of the cylindrical portion 10.

In the present embodiment, as shown in Fig. 2, the gas removal port 10a cut out is formed just below the upper end of the outermost circumference of the baffle 24 and the inner circumferential surface of the cylindrical portion 10 or the fixing portion, so that the baffle 24 and the cylindrical portion are formed. It is comprised so that gas can be discharged out of the gas liquid solid separator 4 between the inner peripheral surfaces of 10. By forming this gas removal port 10a, gas is not charged between the baffle 24 and the inner circumferential surface of the cylindrical portion 10, and the bioreaction water flows in smoothly from the inlet port 13. As shown in Fig. 3, it is preferable to extend the baffle 23 on the outer circumference side longer than the baffles 21 and 22 on the inner circumference side, and to extend the outermost circumference baffle 24 on the lower side than the baffle 23.

In the bioreactor configured as described above, the raw water is introduced into the bioreaction zone S from the distributor 3 and subjected to anaerobic biotreatment, and then flows into the gas liquid solid separator 4 (gas liquid solid separation chamber).

This bioreaction water is introduced into the gas liquid solid separator 4 through the inlet 13 and separated into the gas liquid solids. This gas-liquid solid separation treatment is efficiently carried out by contacting the bioreaction water with the baffles 21 to 24. The gas separated from the bioreaction water is discharged through the gas outlet 5. The treated water flowing through the trough 12 is discharged through the treated water discharge pipe 6.

The sludge settled into the conical section 11 in the gas liquid solid separator 4 gradually moves downward into the conical section 11 and the solid discharge pipe 7 by gravity and falls from the lower end of the discharge pipe 7 toward the bioreaction zone S.

In this embodiment, since the baffles 21 to 24 are provided, the gas liquid solid separation in the gas liquid solid separator 4 is efficiently performed. Since the solids sufficiently concentrated are returned to the bioreaction zone S via the discharge pipe 7, the bioreaction in this bioreaction zone S is performed efficiently.

In the present embodiment, since the gas generated from the sludge in the discharge pipe 7 flows out of the discharge pipe 7 through the gas pocket 16 and the gas removal hole 17, the gas is prevented from being suppressed in the upper portion of the discharge pipe 7 or the lower portion of the conical portion 11. For this reason, the buoyancy of the gas to the sludge is prevented or suppressed, and the sludge is smoothly discharged through the discharge pipe 7. The gas removal hole 17 may be provided in the lower portion of the conical portion 11.

In this embodiment, since the sludge stays in the discharge pipe 7 from the lower portion of the conical portion 11 as described above, the treated water does not flow back into the discharge pipe 7 through the lower end of the discharge pipe 7 or the gas removal hole 17. In addition, since the opening at the lower end of the discharge pipe 7 is inclined upward as indicated by the angle θ ₃ , the reaction gas rising in the container 1 is also prevented from flowing into the discharge pipe 7. The lower end of the discharge pipe 7 may be in the horizontal direction.

Next, preferable dimensions, operating conditions, and the like of each part of the bioreactor of the above embodiment will be described.

The diameter d ₁ of the cylindrical portion 10 of the gas liquid solid separator 4 is preferably 60 to 90%, particularly 70 to 85% of the diameter of the cylindrical container 1.

The height h ₁ of the cylindrical portion 10 is preferably 250 cm or less, particularly 50 to 200 cm.

Inclination angle θ ₁ to the horizontal plane of the conical portion 11 is preferably 45-60 degrees degrees, in particular 50 to 60.

The inclination angle θ ₂ with respect to the horizontal plane of the baffles 21 to 24 is preferably 45 to 60 degrees, particularly 50 to 60 degrees.

The diameter of the solid discharge pipe 7 (average diameter in the case of a non-circular pipe) is 50 to 150% of the diameter of the inlet 13, and is preferably 50 mm or more.

The vertical length (height) h ₂ of the solid discharge pipe 7 is preferably 10% or more, for example, 10 to 70%, more preferably 30 to 80% of the diameter d ₁ of the gas liquid solid separator 4.

Inclined angle to the horizontal plane of the solids discharge pipe 7 is also θ ₄ is preferably 45-135 degrees degrees, particularly 50-130.

It is preferable that the number of the gas removal ports 10a provided in the cylindrical part 10 is four or more.

It is preferable that the number of baffles is 1-10 pieces, especially four pieces.

The length h ₃ of the outermost baffle 24 and the contact portion or fixing portion of the inner circumferential surface of the cylindrical portion 10 to the lower end of the cylindrical portion 10 is 1 to 3 times the diameter of the inlet 13 or the upper and lower widths of the inlet 13, in particular 1.5 to 2 It is preferable to be a ship.

The height h ₄ of the baffle 24 of the outermost circumference is preferably times, 1 to 2 times the height h _3, in particular from 1.25 to 1.5.

The superficial velocity in the vessel 1 is preferably 7 m / ｈ or less. The COD (chemical oxygen demand) load in the container 1 is preferably 30 kg-COD / m 3 · d or less, more preferably 25 kg-COD / m 3 · d or less.

In the above embodiment, the jet nozzle 14 is provided along the inner surface of the conical portion 11, but may be installed vertically as shown in FIG.

Figure 4 (a) is an enlarged view of the upper part of the bioreactor according to another embodiment, Figure 4 (b) is a perspective view of the gas liquid solid separator.

In the embodiment of Fig. 4, the gas liquid solid separator 4A is formed over the entire circumferential direction of the cylindrical portion 10 of the gas liquid solid separator 4A. Inflow of reaction water. Each inlet 13A is formed to surround the outside of the baffle 24.

In the embodiment of Fig. 2, a swirl flow is formed in the gas liquid solid separation chamber by introducing bioreactor water in a tangential direction into the gas liquid separation chamber, so that the gas liquid solid separation is efficiently performed. In Example 4, by increasing the inlet of the bioreaction water, the inflow rate of the bioreaction water is reduced to increase the settling efficiency of the solid in the gas liquid solid separation chamber.

4, the diameter of the solid discharge pipe 7 (average diameter in the case of a non-circular pipe) is 50 to 100% of the upper and lower widths of the inlet 13A, and is preferably 50 mm or more.

The other configuration of Fig. 4 is the same as that of Fig. 2, and like reference numerals denote like parts. In the embodiment of Fig. 4, the inlet 13A is formed in a slit shape, but the shape of the inlet 13A is not particularly limited as long as it is provided over almost the entire circumferential direction of the cylindrical portion 10. In this aspect, the inlet linear velocity of the bioreaction water from inlet 13A is preferably 5 cm / s or less, particularly 1 to 4 cm / s.

In the above embodiment, the containers 1 are all cylindrical, but may be an elliptical cylinder, an angular cylinder, or the like, or may be, for example, a rectangular cylinder. The rectangular cylindrical shape may be a square cylindrical shape or a rectangular cylindrical shape.

In the above embodiment, one gas liquid solid separator 4, 4A is provided on the upper part of the container 1, but several gas liquid solid separators may be provided.

Fig. 5 shows an example thereof, and six gas liquid solid separators 4 are provided in a rectangular container 1A. The treated water from each gas liquid solid separator 4 is collected in a collecting pipe 6A and discharged. Moreover, it is preferable that each gaseous liquid solid separator 4 is supported by the beam-shaped member (not shown in figure) provided in the upper part of container 1A.

The above embodiment is configured to discharge the sludge in the gas liquid solid separator 4 by gravity, but may be sucked out by the pump.

Fig. 6 shows an example, and is configured such that the solids discharge pipe 7 of each gas liquid solid separator 4 is connected to the collection pipe 7A and returned to the bioreaction zone S through the pump P. In Fig. 6, a plurality of gas liquid solid separators 4 are provided, but in Fig. 1, sludge may be taken out from the discharge pipe 7 through a pump. The extracted sludge is preferably returned to the bioreaction zone of the container 1A.

(Bioreactor consisting of a vertical top pipe and a vertical bottom pipe inclined horizontally or downwardly)

FIG. 7 is a cross-sectional view showing an embodiment of a bioreactor composed of an upper tube in which the solid discharge pipe is vertical and a lower tube inclined in a horizontal or downward direction, and is a top cross-sectional view of the same bioreactor as in FIG.

Also in this embodiment, the gas liquid solid separator 4D is installed in the upper portion of the container 1. This gas liquid solid separator 4D has a structure similar to that of the gas liquid solid separator 4 of FIG. 2, except that the gas liquid solid separator 4D is obliquely downward at an inclination angle θ ₅ connected to the upper tube 7a where the solid discharge pipe 7D is vertical and the upper tube 7a. It is a point consisting of the lower pipe 7b extending inclined so as to be inclined, and the jet nozzle 14 and the pipe 15 connected thereto are disposed perpendicular to the axial center portion of the gas liquid solid separator 4D.

The upper tube 7a of the solid discharge tube 7D has an upper end connected to the lower end of the conical portion 11. The lower tube 7b extends obliquely downward from the lower end of the upper tube 7a. A gas pocket 16 and a gas removal hole 17 for discharging gas from the solid discharge pipe 7D are formed in the upper surface of the lower pipe 7b in contact with the upper pipe 7a. The distal opening of the lower tube 7b is in an oblique direction upward by an angle θ ₃ . The gas removal hole may be formed in the lower portion of the upper tube 7a.

The rest of the configuration is the same as in the embodiment of Figs. 1 to 3, and the same reference numerals denote the same parts.

The preferred ranges of the angles θ ₁ , θ ₂ , and θ ₃ in FIG. 7 are as described above. The inclination angle θ ₅ of the lower tube is preferably 0 to 90 degrees, but more preferably 45 to 90 degrees and 60 to 80 degrees.

The preferable range of each dimension of FIG. 7 is also the same as the said Example. The horizontal length of the lower tube 7b is preferably about 5 to 90% of the height h ₂ of the solid discharge tube 7D.

Also in the bioreactor of such a structure of FIG. 7, the effect similar to the bioreactor of FIGS.

Although FIG. 7 comprises the solid discharge pipe 7 in FIGS. 1 to 3 as the upper pipe 7a and the lower pipe 7b, the solid discharge pipe is also used in the bioreactor of FIGS. 4 to 6 as described above. You may comprise with the tube 7b.

In the bioreactor of the present invention, a plurality of baffles having the same center are provided in the gas liquid solid separation chamber in the upper portion of the reaction vessel, and the gas liquid solid separation efficiency is high. For this reason, the bioprocessing reaction efficiency in a container can be improved.

When a solids discharge pipe is installed extending downward from the gas liquid solid separation chamber as in claim 2, the solids stay in the discharge pipe and gradually flow out from the discharge pipe toward the lower portion of the container by gravity. Since the solid content having a greater density (specific gravity) than the liquid stays in the discharge pipe, the liquid does not flow back from the discharge pipe into the gas liquid solid separation chamber, and the gas liquid solid separation efficiency in the gas liquid solid separation chamber is maintained high.

As described in claims 3 and 4, the solid content discharge pipe is inclined, or the lower pipe is horizontally or inclined downward, whereby the solid content easily stays in the discharge pipe. In addition, the opening of the lower end or the distal end of the solid discharge pipe in the transverse direction or the oblique direction upwards prevents the gas generated in the reaction vessel from flowing into the discharge pipe.

Since the solid content stays from the lower part in this gas liquid solid separation chamber to the discharge pipe, the gas can be removed from the retained solid content by forming a gas removal hole in this portion as in claim 5. As a result, the buoyancy of the gas is prevented or suppressed from being applied to the retained solids, and the solids smoothly escape through the discharge pipe.

In the case where a lower pipe inclined obliquely horizontally or downwardly is provided in the lower portion of the solid discharge pipe as in claim 6, it is possible to remove the gas from the solid retained by satisfactorily forming a gas removal hole in the lower or lower pipe of the upper pipe. Can be.

By flowing the liquid in the tangential direction between the baffle at the outermost circumference and the inner surface of the gas liquid solid separation chamber as described in claim 7, a swirl flow of liquid is formed in the gas liquid solid separation chamber to sufficiently separate the gas liquid solid. .

As described in claim 8, by forming a gas removal port in the cylindrical portion so that gas escapes from the portion between the outermost baffle and the inner surface of the gas liquid solid separation chamber, the gas is prevented from filling the contact portion. In addition, the liquid is smoothly introduced into the gas liquid solid separation chamber.

Claims (8)

The vessel in which the bioreaction treatment of the liquid is carried out, A gas liquid solid separation chamber installed at an upper portion of the vessel and into which liquid is introduced from the vessel to settle separation of solids and float separation of gases in the liquid;體 分離 室) In the bioreactor having a (生物 反應 器), In the gas-liquid solid separation chamber, a plurality of baffles having an inclined shape having the same center and being installed at regular intervals from each other and having a smaller diameter toward the bottom are installed. Characterized by a bioreactor. The method of claim 1, The gas liquid solid separation chamber includes an upper cylindrical portion and a conical portion continuous to the lower side of the cylindrical portion. Solids discharge pipe for flowing out the solid precipitated downward in the gas liquid solid separation chamber by gravity from the gas liquid solid separation chamber extending downward from the lower end of the conical portion, The vertical length h ₂ of the solid discharge pipe is not less than 0.1 times the inner diameter d ₁ of the cylindrical portion. Characterized by a bioreactor. The method of claim 2, The solid discharge pipe extends inclined with respect to the vertical direction, The opening of the lower end of the solid discharge pipe is in a horizontal direction or obliquely upward Characterized by a bioreactor. The method according to claim 2 or 3, A gas removal hole is formed in the lower portion of the gas liquid solid separation chamber or the upper portion of the solid discharge pipe to allow gas in the gas liquid solid separation chamber or the solid discharge pipe to flow out of the gas liquid solid separation chamber or the solid discharge pipe. Bioreactor characterized in that. The method of claim 2, The solid discharge pipe and the upper pipe is installed extending in the vertical direction, It is connected to the upper tube is made of a lower tube extending inclined in the horizontal direction or downwards, The opening of the distal end of the lower tube is in a direction obliquely transversely or upwardly Characterized by a bioreactor. The method of claim 5, And a gas removal hole for discharging the gas in the solid discharge pipe out of the solid discharge pipe in a lower portion of the upper pipe or in the lower pipe. The method according to any one of claims 1 to 6, The upper end of the outermost baffle is continuous to the inner surface of the gas liquid solid separation chamber, An inlet portion is formed between the inner surface of the gas liquid solid separation chamber and the baffle at the outermost circumference such that the liquid in the vessel flows in a substantially tangential direction into the gas liquid solid separation chamber. Characterized by a bioreactor. The method of claim 7, wherein And a gas removal port for allowing gas to flow out from the inner surface of the gas liquid solid separation chamber and the outermost circumference baffle to the outer side of the cylindrical portion.

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