KR20070023466A - Method of extracting and refining an anti-oxidizing agent from the Solanum nigrum L and method of producing a cosmetics using the same - Google Patents

Method of extracting and refining an anti-oxidizing agent from the Solanum nigrum L and method of producing a cosmetics using the same Download PDF

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KR20070023466A
KR20070023466A KR1020050078136A KR20050078136A KR20070023466A KR 20070023466 A KR20070023466 A KR 20070023466A KR 1020050078136 A KR1020050078136 A KR 1020050078136A KR 20050078136 A KR20050078136 A KR 20050078136A KR 20070023466 A KR20070023466 A KR 20070023466A
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antioxidant
extracting
solution
sepadex
ingredient
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KR1020050078136A
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Korean (ko)
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김효주
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주식회사 하나코스
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/81Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/51Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/53Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

Abstract

본 발명은 까마중(Solanum nigrum L)의 줄기, 뿌리로부터 여드름, 아토피성 피부염 및 각종 피부 질환의 치료에 효과가 있는 항산화 성분의 추출 및 정제 방법을 제공한다. 본 발명의 실시 예에 의하면, 까마중의 줄기, 뿌리 1kg을 잘게 분쇄하고 정제수 10,000㎖를 가한 다음 100℃에서 20분간 끓인 다음 이것을 300mesh를 이용하여 여과하고 그 여과 용액을 초고속 원심분리기를 이용하여 25℃에서 10,000rpm의 속도로 5분간 원심분리기한다. 침전물을 버리고 용액을 취하여 Sepadex G-100을 충전물로 한 Column을 이용하여 정제한다. 이때 충전물로 사용한 Sepadex G-100의 높이는 30cm로 한다. 이렇게 정제하여 얻어진 활성 성분(항산화 성분)을 동결 건조시킨다. 동결 건조시킨 내용물(항산화 성분)을 0.3v/v% 에탄올 용액과 0.3v/v% 부틸렌그라이콜 용액을 만들어 기능성 화장품용 원료로 사용한다. 일반적으로 알려진 항산화제인 Tocopherol류는 천연 항산화제로서 안전하기는하나 단독으로는 산화 연쇄반응 저지 능력이 낮고, 고가인 단점이 있다. 이때 본 용액은 각종 생약이나 식용식물의 추출물 등에서 보다 안정하고, 항산화력이 강한 천연 항산화제의 개발 필요성이 대두되는 현 시점에서 획기적인 발명품이 아닐 수 없다.The present invention provides a method for extracting and purifying an antioxidant component effective in treating acne, atopic dermatitis and various skin diseases from the stem and root of Solanum nigrum L. According to an embodiment of the present invention, finely pulverize 1 kg of stem and root in crow, add 10,000 ml of purified water, boil at 100 ° C. for 20 minutes, filter it using 300 mesh, and filter the filtrate at 25 ° C. using an ultrafast centrifuge. Centrifuge for 5 minutes at 10,000 rpm. Discard the precipitate, take the solution and purify using a column containing Sepadex G-100. At this time, the height of Sepadex G-100 used as a filling is 30cm. The active ingredient (antioxidant ingredient) obtained in this manner is lyophilized. The freeze-dried contents (an antioxidant component) are used as a raw material for functional cosmetics by making 0.3v / v% ethanol solution and 0.3v / v% butylene glycol solution. Tocopherols, which are generally known as antioxidants, are safe as natural antioxidants, but have low disadvantages and low cost of preventing oxidative chain reaction alone. At this time, this solution is a revolutionary invention at the present time when the need for the development of natural antioxidants, which are more stable and more powerful in extracts of various herbs and edible plants.

Description

까마중으로부터 항산화제를 추출, 정제하는 방법 및 까마중 추출물을 이용한 화장료의 개발{Method of extracting and refining an anti-oxidizing agent from the Solanum nigrum L and method of producing a cosmetics using the same}Method of extracting and refining an anti-oxidizing agent from the Solanum nigrum L and method of producing a cosmetics using the same}

본 발명은 까마중(Solanum nigrum L)의 줄기, 뿌리로부터 항산화 성분을 추출하고 정제하는 방법과 이 성분을 이용하여 피부질환을 치유할 수 있고 피부노화를 방지할 수 있는 각종 화장품의 제조 방법에 관한 것이다.The present invention relates to a method for extracting and purifying antioxidants from the stems and roots of Solanum nigrum L and to a method for producing various cosmetics that can heal skin diseases and prevent skin aging using these components. .

일반적으로, 강력한 태양 아래서 자란 나무는 태양의 자외선으로부터 자기 자신을 보호하기 위하여 많은 활성산소(SOD)를 억제하는 물질을 자체적으로 생성하는 것으로 알려져 있다. 이렇듯이 나무가 자신을 지키기 위하여 만든 항산화 물질은 인간의 건강을 지키기 위한 필요 성분이 되는 것이다.In general, trees grown under the strong sun are known to produce substances that inhibit many free radicals (SOD) in order to protect themselves from the sun's ultraviolet rays. As such, the antioxidants that trees make to protect themselves become necessary ingredients to protect human health.

활성산소(SOD)는 공기 중에 있는 일반적인 산소보다도 여러 물질과 반응하기 쉬운 형태의 산소로서 여러 가지 종류가 있다. 활성산소(SOD)는 물질에 쉽게 붙어 그 물질들을 산화시키고 다른 형태로 변화시킨다.Active oxygen (SOD) is a type of oxygen that is more easily reacted with various substances than general oxygen in the air. Active oxygen (SOD) easily attaches to a substance, oxidizing it and transforming it into another form.

활성산소(SOD)에는 백혈구가 만든 활성산소(SOD)와 같이 인간에게 필요한 활성산소(SOD) 이외에 인간에게 해가 되는 활성산소(SOD)도 있다. 인간은 산소가 없 다면 살아갈 수 없다. 하지만 산소가 너무 과하면 오히려 인간에게 커다란 해가 될 수도 있다. 물질이 타거나 금속 표면에 녹이 Tm는 현상은 산소가 있기 때문이며 인간은 호흡을 통하여 산소를 몸속에 집어넣고 그 산소를 사용해 신체에 필요한 에너지를 만들거나 여러 물질을 만들고 필요 없는 것을 파괴한다. 이것을 생물의 대사라 일컫는다.In addition to active oxygen (SOD) necessary for humans, such as active oxygen (SOD) made by white blood cells, there are also active oxygen (SOD) that is harmful to humans. Humans cannot live without oxygen. But too much oxygen can be a huge harm to humans. Tm burns or melts on metal surfaces because of the presence of oxygen. Humans breathe oxygen into their bodies and use that oxygen to create energy for the body or to create materials and destroy things that are not needed. This is called metabolism of living things.

활성산소는 이처럼 정상적인 대사에서 발생하는데, 인간의 신체가 활성산소로부터 몸을 보호하기 위하여 활성산소를 독이 되지 않는 물질인 산소나 물로 변화시키기도 하며, 비타민 E, 비타민 A, 비타민 C에 의해 활성산소를 없애준다.Free radicals occur in this normal metabolism. In order to protect the body from free radicals, the human body converts free radicals into oxygen or water, which are not poisonous substances, and free radicals by vitamin E, vitamin A and vitamin C. Get rid of it.

한편, 까마중은 신진대사를 돕는 아스파라긴, 피부의 혈행을 촉진시켜 신진대사를 활발히 하는 사포닌, 피부를 부드럽게하고 재생을 촉진시켜주는 카로틴을 비롯하여 각종 효소를 많이 함유하여 간장과 신장의 기능을 강화시켜 혈액이 정화되므로 여드름도 치료되어 피부가 좋아진다.On the other hand, it contains a lot of enzymes, including asparagine, which helps metabolism, saponin, which promotes blood circulation, and carotene, which softens and promotes regeneration. This cleanses, so acne is also treated and improves skin.

본 발명의 제 1 목적은 기존 추출방법에서 까마중의 줄기와 뿌리를 이용하여 오랜 시간에 걸쳐서 추출이 이루어져왔다. 그런데 본 발명의 경우에는 컬럼 및 초고속 원심분리기를 이용하여 불순물을 제거하고 활성성분을 보다 순수하게 추출하는 방법을 제공하는데 있다.The first object of the present invention has been extracted over a long time using the stem and root of the crow in the existing extraction method. However, the present invention provides a method for removing impurities and extracting the active ingredient more purely using a column and an ultrafast centrifuge.

본 발명의 제 2 목적은 본 발명에 의한 액상 추출물의 항산화 성분을 이용하여 화장료를 만드는 방법을 제공하는데 있다.A second object of the present invention is to provide a method of making cosmetics using the antioxidant component of the liquid extract according to the present invention.

상기와 같은 제 1의 목적을 달성하기 위해서, 본 발명은,In order to achieve the first object as described above, the present invention,

까마중의 줄기, 뿌리 1kg을 잘게 분쇄하고 정제수 10,000㎖를 가한 다음 100℃에서 20분간 끓인 다음 이것을 300mesh를 이용하여 여과하고 그 여과 용액을 초고속 원심분리기를 이용하여 25℃에서 10,000rpm의 속도로 5분간 원심분리한다. 침전물을 버리고 용액을 취하여 Sepadex G-100을 충전물로 한 Column을 이용하여 정제한다. 이때 충전물로 사용한 Sepadex G-100의 높이는 30cm로 한다. 이렇게 정제하여 얻어진 활성 성분(항산화 성분)을 동결 건조시킨다.1kg of stalks and roots in the black chopped finely, 10,000ml of purified water was added, boiled at 100 ° C for 20 minutes, filtered using 300mesh, and the filtrate was filtered for 5 minutes at 25 rpm at a speed of 10,000rpm using a high speed centrifuge. Centrifuge. Discard the precipitate, take the solution and purify using a column containing Sepadex G-100. At this time, the height of Sepadex G-100 used as a filling is 30cm. The active ingredient (antioxidant ingredient) obtained in this manner is lyophilized.

동결 건조시킨 활성 성분(항산화 성분)을 가지고 ①0.3v/v% 에탄올 용액과 ②0.3v/v% 부틸렌그라이콜 용액을 만든다.Using the freeze-dried active ingredient (antioxidant), make ①0.3v / v% ethanol solution and ②0.3v / v% butylene glycol solution.

상기와 같은 제 2의 목적을 달성하기 위해서, 본 발명은In order to achieve the second object as described above, the present invention

에탄올 5∼20%, 일정량의 계면활성제, 보존료, 향료, 색소, 완충제 1% 내외, 소정의 미용 성분과 까마중으로부터 추출한 항산화 성분 0.1∼20%, 정제수 58∼ 90%를 혼합, 용해시킨 후 여과하여 화장료 가운데 화장수를 제품화하는 것을 특징으로 하는 까마중으로부터 추출, 정제한 항산화 성분을 이용한 제조 방법을 제공한다.Ethanol 5-20%, a certain amount of surfactant, preservatives, fragrances, pigments, buffer 1%, and about 0.1% to 20% of the antioxidant component extracted from the predetermined cosmetic ingredient and the black, purified water 58-90% mixed, dissolved and filtered Provided is a manufacturing method using an antioxidant component extracted and purified from a crow, characterized in that the lotion is formulated in a cosmetic.

까마중으로부터 추출한 항산화 성분을 제 1의 목적에 맞게 추출, 정제한 다음, 유상 성분이 되는 스테아린산, 스테아릴알코올, 스쿠알란, 자기유화형모노스테아린산글리세린 등을 20% 내외로 사용하고 보습제로서 프로필렌글라이콜, 글리세린, 향료, 보존제, 등을 15% 내외로 사용하고 까마중으로부터 추출한 항산화 성분 0.1 ∼20%와 함께 정제수 40∼60%에 투입 후 수상을 서서히 교반하면서 혼합시켜 준 후 제품화하는 것을 특징으로 하는 까마중으로부터 추출, 정제한 항산화 성분을 약유성 유액류(크림, 에멀젼, 에센스 등)의 제조 방법을 제공하는 것을 특징으로 한다.After extracting and purifying the antioxidant component extracted from the yam according to the first purpose, 20% of stearic acid, stearyl alcohol, squalane, self-emulsifying monostearate, etc., which are oil phase components, is used, and propylene glycol is used as a moisturizing agent. , Glycerin, fragrance, preservatives, etc. are used in about 15%, and 0.1 to 20% of the antioxidant component extracted from the crows, and then added to purified water 40 to 60%, the aqueous phase is slowly mixed with agitation and then commercialized. It is characterized by providing a method for producing weak oily emulsions (creams, emulsions, essences, etc.) extracted and purified from the antioxidant component.

상기에서 언급한 바와 같이 본 발명에 따르면 까마중에서 추출, 정제한 항산화 성분을 이용하여 화장품을 제조함으로써, 기종 시판되고 있는 여드름 치료용 화장품이 갖고 있는 단점인 피부 알러지와 부작용 발생 문제점을 보완하고, 각종 질병 및 피부 노화의 주원인인 활성산소로부터 피부를 보호할 수 있게 한다.As mentioned above, according to the present invention, cosmetics are manufactured using antioxidant components extracted and purified in a crow, thereby compensating for the problem of skin allergy and side effects, which are disadvantages of commercial acne treatment cosmetics, It protects the skin from free radicals which are the main cause of disease and skin aging.

DPPH free radical 소거활성법은 항산화제가 안정한 free radical DPPG 와 재반응하고 그것은 1,1-diphenyl-2-picrylhydrazine로 변환되는 정도를 조사하는 것으로 이러한 방법은 많은 식물 추출물들의 항산화 활성을 탐색하는데 이용되어진다.DPPH free radical scavenging activity is used to investigate the antioxidant activity of many plant extracts by investigating the extent to which the antioxidants react with stable free radical DPPG and convert it to 1,1-diphenyl-2-picrylhydrazine. .

본 실험의 IC50(㎍/㎖)값은 700여종의 식물 추출물 중 102종이 200 ㎍/㎖ 이하의 활성을 나타내었다는 보고를 미루어보면, 까마중의 전 식물체 및 부위별 추출물의 IC50 값은 62∼145 ㎍/㎖의 범위로 DPPG free radical 소거활성이 높은 것으로 평가되었다.IC 50 (㎍ / ㎖) value of the experiments In the delayed report had indicated the 700 species of plant extracts 102 paper 200 ㎍ / ㎖ or less of the active, the former plant, and IC 50 value of the cuts of the extract of Solanum nigrum has 62~ It was evaluated that DPPG free radical scavenging activity was high in the range of 145 ㎍ / mL.

Claims (4)

까마중의 줄기, 뿌리 1kg을 잘게 분쇄하고 정제수 10,000㎖를 가한 다음 100℃에서 20분간 끓인 다음 이것을 300mesh를 이용하여 여과하고 그 여과 용액을 초고속 원심분리기를 이용하여 25℃에서 10,000rpm의 속도로 5분간 원심분리한다.1kg of stalks and roots in the black chopped finely, 10,000ml of purified water was added, boiled at 100 ° C for 20 minutes, filtered using 300mesh, and the filtrate was filtered for 5 minutes at 25 rpm at a speed of 10,000rpm using a high speed centrifuge. Centrifuge. 침전물을 버리고 용액을 취하여 Sepadex G-100을 충전물로 한 Column을 이용하여 정제한다. 이때 충전물로 사용한 Sepadex G-100의 높이는 30cm로 한다. 이렇게 정제하여 얻어진 활성 성분(항산화 성분)을 동결 건조시킨다.Discard the precipitate, take the solution and purify using a column containing Sepadex G-100. At this time, the height of Sepadex G-100 used as a filling is 30cm. The active ingredient (antioxidant ingredient) obtained in this manner is lyophilized. 이렇게 동결 건조한 활성 물질을 0.3v/v% 에탄올 용액으로 만드는 것을 특징으로 하는 까마중으로부터 유용성분을 추출, 정제하는 방법The method of extracting and purifying useful components from crows, characterized in that the freeze-dried active substance is made into 0.3v / v% ethanol solution. 까마중의 줄기, 뿌리 1kg을 잘게 분쇄하고 정제수 10,000㎖를 가한 다음 100℃에서 20분간 끓인 다음 이것을 300mesh를 이용하여 여과하고 그 여과 용액을 초고속 원심분리기를 이용하여 25℃에서 10,000rpm의 속도로 5분간 원심분리한다. 침전물을 버리고 용액을 취하여 Sepadex G-100을 충전물로 한 Column을 이용하여 정제한다. 이때 충전물로 사용한 Sepadex G-100의 높이는 30cm로 한다. 이렇게 정제하여 얻어진 활성 성분(항산화 성분)을 동결 건조시킨다.1kg of stalks and roots in the black chopped finely, 10,000ml of purified water was added, boiled at 100 ° C for 20 minutes, filtered using 300mesh, and the filtrate was filtered for 5 minutes at 25 rpm at a speed of 10,000rpm using a high speed centrifuge. Centrifuge. Discard the precipitate, take the solution and purify using a column containing Sepadex G-100. At this time, the height of Sepadex G-100 used as a filling is 30cm. The active ingredient (antioxidant ingredient) obtained in this manner is lyophilized. 이렇게 동결 건조한 활성 물질을 0.3v/v% 1,3-부틸렌글라이콜 용액으로 만드는 것을 특징으로 하는 까마중으로부터 유용성분을 추출, 정제하는 방법Method for extracting and purifying the useful ingredient from the hood, characterized in that the lyophilized active substance is 0.3v / v% 1,3-butylene glycol solution 까마중으로부터 항산화 성분을 추출한 다음, 에탄올 5∼20%, 일정량의 계면활성제, 보존료, 향료, 색소, 완충제 1% 내외. 소정의 미용 성분과 까마중으로부터 추출한 항산화 성분 0.1∼20%, 정제수 58∼90%를 혼합, 용해시킨 후 여과하여 화장료 가운데 화장수를 제품화 하는 것을 특징으로 하는 까마중 추출물을 이용한 화장수 제조 방법.After extracting the antioxidant component from the ethanol, ethanol 5-20%, a certain amount of surfactant, preservatives, fragrances, pigments, buffer 1% or so. A method of producing a lotion using a extract of a camouflage, characterized in that a cosmetic product is formulated by mixing and dissolving a predetermined cosmetic ingredient with 0.1 to 20% of an antioxidant component and 58 to 90% of purified water extracted from a crow and then filtration. 까마중으로부터 항산화 성분을 추출한 다음, 유상 성분이 되는 스테아린산, 스테아릴알코올, 스쿠알란, 자기유화형모노스테아린산글리세린 등을 20% 내외로 사용하고 보습제로서 프로필렌글라이콜, 글리세린, 향료, 보존제 등을 15% 내외를 까마중으로부터 추출한 항산화 성분 0.1∼20%와 함께 정제수 40∼60%에 투입 후 수상을 서서히 교반하면서 혼합시켜 준 후 제품화하는 것을 특징으로 하는 까마중 추출물을 이용한 약유성 유액류(크림, 에멀젼, 에센스 등)의 제조 방법.After extracting the antioxidant component from the root, use about 20% of stearic acid, stearyl alcohol, squalane, self-emulsifying monostearic acid, etc., which are oil phase components, and use 15% of propylene glycol, glycerin, perfume, preservative, etc. as a moisturizing agent. Weak oil emulsion (Cream, Emulsion, Essence) using extracts of black and white extract which is characterized in that it is mixed with 0.1 to 20% of antioxidant components extracted from the inside and outside into 40 to 60% of purified water, and then mixed with water phase while stirring slowly. And the like).
KR1020050078136A 2005-08-23 2005-08-23 Method of extracting and refining an anti-oxidizing agent from the Solanum nigrum L and method of producing a cosmetics using the same KR20070023466A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100847478B1 (en) * 2007-04-13 2008-07-21 에이비씨나노텍 주식회사 Composition for functional soap comprising gold or silver nanoparticle and extraction polymer of black nightshade as effective ingredients, and production method thereof
CN105147566A (en) * 2015-10-10 2015-12-16 吴优 Anti-wrinkle and moisture-holding beauty cream and preparation method thereof
CN106509579A (en) * 2016-11-24 2017-03-22 张锦超 Preparation method for nigrum fruit products

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100847478B1 (en) * 2007-04-13 2008-07-21 에이비씨나노텍 주식회사 Composition for functional soap comprising gold or silver nanoparticle and extraction polymer of black nightshade as effective ingredients, and production method thereof
CN105147566A (en) * 2015-10-10 2015-12-16 吴优 Anti-wrinkle and moisture-holding beauty cream and preparation method thereof
CN106509579A (en) * 2016-11-24 2017-03-22 张锦超 Preparation method for nigrum fruit products

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