KR20070022907A - Composition and food of cancer chemoprevention and treatment with extracts of Wasabia japonica - Google Patents

Abstract

The present invention relates to a composition for preventing and treating cancer and health food comprising a horseradish extract, and more particularly, by confirming the action of the horseradish extract as a quinone reductase activity inducer to prevent cancer and It can be applied to therapeutic compositions, and wasabi extract with such effects is mixed with various foods such as chewing gum, candy, biscuits, ice cream, beverages and chocolates to be easily consumed anytime, anywhere to a wide range of adolescents, adults and the elderly. It relates to a cancer preventive and therapeutic composition and health food comprising horseradish extract to be able to expect a lasting drinking effect.

Wasabi extract, quinone reductase, cancer prevention

Description

Composition and food of cancer chemoprevention and treatment with extracts of Wasabia japonica}

  1 is a graph showing the effect of inducing quinone reductase activity at the concentration of horseradish extract 10 ~ 100 ㎍ / ml using mouse liver (mouse, hepatoma cell: Hepa 1c1c7 cell).

Figure 2 is a figure showing the effect of wasabi extract inducing quinone reductase mRNA expression.

The present invention relates to a composition for preventing and treating cancer and health food comprising a horseradish extract, and more particularly, by confirming the action of the horseradish extract as a quinone reductase activity inducer to prevent cancer and It can be applied to therapeutic compositions, and wasabi extract with this effect is blended with various foods such as chewing gum, candy, biscuits, ice cream, beverages, and chocolates to provide a wide range of youth, adults and seniors anytime, anywhere. It relates to a cancer preventive and therapeutic composition and health food comprising horseradish extract that can be ingested so that a continuous drinking effect can be expected.

Cancer is one of the causes of death in Korea, and 51.1% of all cases die within a year after knowing the disease, and the medical treatment has steadily improved, but medical doctors say that the cure rate will not exceed 50% by the end of the 20th century. It is reported that there are 8 million cases worldwide each year, 5 million of which are the worst of humanity.

Carcinogens that cause such cancers include food, smoking, ultraviolet rays, chemicals, and other environmental factors, and the reality is that humans have to be exposed to these carcinogens by will or by other means.

Therefore, there is an urgent need for the development of cancer prevention substances, and the development of such cancer prevention substances can be very useful for inhibiting or treating all diseases as well as cancer. For this reason, as the development of cancer prevention substances is recognized as a new direction of cancer research and attention is focused, the development of cancer prevention substance materials is active not only in the real medicine field, but also in the food chemical field, so that research on natural product materials is active. In fact, it is also being introduced into the food sector.

As a result, many analytical systems using microorganisms or animal cells have been established and utilized for the research and development of new cancer prevention substances. Molecular biological studies of pure separation of active ingredients and mechanisms of action have also been actively conducted.

In particular, Phase II detoxification enzymes exhibit specific cytotoxicity against various cancer cells, and have a protective action against damage to DNA by carcinogens, mutagenic substances, and reactive oxygen species such as those having anticancer function. Known.

These second phase detoxifying enzymes include glutathione sulfur transferase (GST), NAD (P) H: quinone reductase (QR), and epoxide hydrolase. ), Glucuronosyltransferase, aldehyde reductase and the like.

Of the Phase II detoxification enzymes, quinone reductase (EC 1.6.99.2) is an enzyme that blocks interaction with DNA by mutations or carcinogens, and uses NAD (P) H. It is a flavoprotein that catalyzes the reduction of quinones. The quinone reductase has a molecular weight of 54,000 daltons, a homodimer of two monomers of the same structure, each of which consists of 273 amino acids, and an active site of flavin adenine dinucleotide. (flavoprotein) containing (flavin adenine dinucleotide, FAD), also known as menadione reductase, vitamin K reductase, DT-diaphorase Is named).

Such quinone reductase has a number of characteristics as follows. Firstly, NAD (P) H is used as an electron donor, and secondly, two electrons are not moved to form a semiquinone, and third, strong anticoagulant such as dicoumarol prevents strong inhibition. Fourth, in many cells and tissues are induced by a variety of foreign substances.

In particular, these quinone reductases have a protective effect on the quinones themselves, and are commonly induced with other cancer prevention enzymes and are induced by many compounds with anticancer activity. Among the detoxification enzymes, it can be selected as a representative enzyme for the search for cancer prevention substance [Lind, C., Vady, H. and Ernster, L. 1978, Arch. Biochem. Biophys. 190: 97-108; Wefers, H., Komai, T., Talalay, P. and Sies, H. 1984. FEBS Lett. 169: 63-66.

Meanwhile, various kinds of animal cells can be used to more quickly, simply and accurately measure quinone reductase. In the present invention, a mouse liver cell line (Hepa 1c1c7 murine hepatoma cell line) was introduced.

Substances exhibiting quinone reductase induced activity by these cell lines include phenolic antioxidants, lipophilic azo dyes, and sulfur compounds. Among them, quinone reductase-inducing activity is reported to be strong in cruciferous plants (broccoli, cabbage, kale, cauliflower, etc.), liliaceae, plants (onion, garlic) and plants of the genus Vaccinium. have.

Among the above, in particular, the most active researches to date have been conducted. The strong inducer is sulforaphane (sulforaphane), an active substance of broccoli, which is a kind of isothiocyanate, and a lot of research has been conducted. Gerhauser, C., You, M., Liu, J., Moroarty, RM, Hawthorne, M., Mehts, RG, Moon, RC and Pezzuto, J.M. 1997. Cancer Res. 57: 272-278; Posner, G.H., Cho, C.G., Green, G.V., Zhang, Y. and Talalay, P. 1994, J. Med. Chem. 37: 170-176.

On the other hand, wasabi ( Wasabia japonica, W. koreana ) and Japanese horseradish ( Japanese horseradish ) are plants belonging to the cruciferous family, Japanese wasabi ( Wasabia japonica ) and horseradish. [Horseradish radish, Horseradish, Cocholearia armoracia L. ].

It is a perennial herb that is wild or cultivated in the mountains and valleys of Korea's Ulleungdo Island and Japan. It is about 30 cm in height, the rhizome is thickened, and the root leaves are gathered and revived in several layers. The folies are heart-shaped, fermented, and the flowers bloom in May-June with white cruciferous flowers at the end of the stems or total flowers, and the fruit has the beak-shaped projections at the end of each branch. Root is used as a herbal medicine and is called San Kyu-Geun ( Wasabiae Rhizoma ). The main components include rhizome, sinigrin, arylisothiocyanate, and butylisothiocyanate.

When the plant is wound or peeled off the root, the plant has a unique smell and spicy taste. Due to this flavor, the plant has been properly processed and used as an appetite promoting or digestion promoting food.

The main ingredient of the above-mentioned flavor is found to be arylisothiocyanate, which is the main volatile component of horseradish essential oil. Such arylisothiocyanates are not present from the beginning of the plant, but are present in an inactive state of cinigrin, which is a kind of thioglucoside in the tissue, and once the tissue is cut or injured, it is a myrosulpa as an enzyme contained in the tissue. As the synigrin is hydrolyzed by the action of myrosinase, a complex enzyme of myrosulfatase and thioglucosidase, glucose, potassium hydrogen sulfate and arylisothiocyanate are produced.

In addition to promoting appetite and digestion due to taste and aroma, arylisothiocyanate has been reported for antibacterial effects. Recent research trends using spice are not only food additives but also the cosmetics industry and aromatherapy. Research is being attempted at

From the past, horseradish has been used to increase appetite, sourness, preservation, and sterilization, especially in Japan, where it is used for the poisoning of fish and algae.

On the other hand, wasabi (wasabi) kimchi manufacturing method is disclosed [Korean Patent Registration No. 10-0483249], wasabi storage bowl is known [Korean Utility Model Registration No. 20-0356802], from horseradish A stock pest control agent containing an extracted horseradish essential oil as an active ingredient is disclosed [Korean Patent Registration No. 10-0391827].

However, the technology has not yet been widely used, most of the studies using horseradish is the study on the antibacterial effect of horseradish extract, aryl isothiocyanate content of horseradish root or its components.

In addition, despite many studies on natural substances that exhibit cancer prevention effects today, there is no case published domestic and international studies on the horseradish extract has a cancer prevention effect as shown in the present invention.

Accordingly, the inventors of the present invention has a superior cancer prevention effect by wasabi extract induces the activity of quinone reductase of a higher or equivalent degree compared to the conventional inducers of quinone reductase. It was confirmed to complete the present invention.

Therefore, the present invention not only saves the various physiological activities of wasabi itself, but also quinone reductase by breaking from the conventional method used in the conventional wasabi as a spice to limit appetite, sour stomach, antiseptic, sterilization, etc. It is an object of the present invention to provide a composition for preventing and treating cancer, comprising horseradish extract, which is used as a composition having an effect of preventing and treating cancer by inducing its activity, and also being easily commercialized by adding and blending to various foods. .

The present invention features a composition for preventing and treating cancer comprising horseradish extract.

The present invention is characterized by comprising horseradish extract and includes health foods that induce the activity of quinone reductase (Quinone reductase).

Hereinafter, the present invention will be described in detail.

The present invention can be applied to the composition for the prevention and treatment of cancer by confirming the action of quinone reductase activity inducing substance of horseradish extract, the horseradish extract having this effect chewing gum, candy, biscuits, ice cream, beverages and The present invention relates to a composition for preventing and treating cancer, including horseradish extract, which is formulated together with various foods such as chocolate and can be easily consumed anytime and anywhere to a wide range of adolescents, adults, and the elderly.

The present invention relates to a new use of horseradish extract to use wasabi extract as an active inducer of quinone reductase as a composition having a cancer prevention and treatment effect and to apply it to food.

As the wasabi extract of the present invention, Japanese wasabi and domestic wasabi may be used, and water, lower alcohol having 1 to 6 carbon atoms, or a mixture thereof may be used as an extraction solvent.

In the present invention, the efficacy was measured using domestic wasabi, using a method of extracting wasabi was extracted by using ethanol as a solvent, ultrasonic irradiation. That is, the method of obtaining wasabi extract in more detail in the present invention as follows.

First, the root of horseradish was sampled with liquid nitrogen, and it was made into a powder by a grinder, and then ethanol was added and extracted two or three times with ultrasonic waves for 20 to 40 minutes, and then the filtrate was filtered using Toyo filter No. 2. The resultant was concentrated under reduced pressure at 45 to 55 ° C. with a rotary vacuum evaporator, and the concentrate was lyophilized to obtain a light green horseradish dried powder, which was stored at a temperature of −70 ° C.

As a result of measuring the effect of inducing quinone reductase activity against horseradish extract prepared by the manufacturing method as described above, it was confirmed that the cancer prevention effect is excellent, and this can be applied to chewing gum, candy, biscuits, ice cream, beverages, chocolate, etc. As such, by combining together in the food, a wide range of adolescents, adults and seniors can be easily consumed anytime, anywhere, and can be expected to have a continuous drinking effect.

As in the present invention, when the horseradish extract is applied to food, it is preferable to use the horseradish extract essentially 0.1 to 1.0% by weight based on the total composition. If the content is less than 0.1% by weight, it is not desirable to achieve the desired effect of the present invention, while if it is added in excess of the above range, it is uneconomical and affects the taste and color of the finished product. It is not desirable because it can.

Cancer preventive and therapeutic compositions comprising horseradish extract of the present invention can be administered orally or parenterally, for example, intravenously, subcutaneously, intraperitoneally or topically, during clinical administration, and in the form of general medicines and health foods. Can be used as

Formulations for oral administration such as tablets, troches, lozenges, aqueous or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elixirs. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin for preparation in formulations such as tablets and capsules; Excipients such as dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. Capsules contain liquid carriers, such as fatty oils, in addition to the substances mentioned above.

In addition, cancer preventive and therapeutic compositions comprising horseradish extract of the present invention can be administered parenterally, parenteral administration is by subcutaneous injection, intravenous injection, intramuscular injection or intramuscular injection injection method. To formulate into a parenteral formulation, horseradish extract is mixed in water with a stabilizer or buffer to prepare a solution or suspension which is formulated in unit dosage forms of ampoules or vials.

In addition, the dosage of the active ingredient is generally 1 to 50 mg / day per kg body weight of an adult patient, preferably 5 to 20 mg / day, 1 to several times at regular intervals according to the judgment of the doctor or pharmacist Preferably, it can be dividedly administered 2-3 times a day.

In addition, the present invention includes a health food containing horseradish extract as an active ingredient. The food is a food product prepared by adding wasabi extract to food materials such as beverages, teas, spices, gums, confectionery, or the like, encapsulated, powdered, or suspension, and when ingested, has a specific health effect. Meaning, unlike the general medicine has the advantage that there is no side effect that can occur when the long-term use of the drug using food as a raw material.

Hereinafter, the present invention will be described in more detail with reference to Examples. The present invention is not limited by the following Examples.

Reference Example: Animal cell line culture and experimental animal breeding

Mouse-derived hepatocytes (Hepa 1c1c7 cell line) used in the present invention were purchased from the American Type Culture Collection (ATCC CRL 2026), 2 mM glutamine (glutamine), 100 U Minimum essential medium alpha modification (α-MEM) medium containing / ml penicillin G, 100 μg / ml streptomycin, 10% fetal bovine serum, etc. Was used in the experiment while subcultured in a CO ₂ incubator at 37 ℃ 5% CO ₂ /95% air environment.

On the other hand, the experimental animals were used to receive a three-week-old Sprague-Dawley rat females of about 85g body weight from the animal nursery of the National Institutes of Health. The animal breeding environment was maintained under the conditions of artificial lighting (8 am-8 pm) at an interval of 12 hours, roughness 300-500 lux, temperature 23 ± 1 ° C, exhaust 10-18 times / h, and solid feed (Samyang feed) ) And drinking water freely.

Preparation Example: Isolation of Wasabi Extract

The raw material [Japanese wasabi, Wasabia japonica ] used in the present invention was purchased from a cultivated farmhouse located in Cheorwon-gun, Gangwon-do, washed and dried, and cut into approximately 2 × 2 × 2 (width × length × thickness, cm) size liquid nitrogen. Powdered horseradish was immediately pulverized using a pulverizer, and ethanol, an extraction solvent, was used as a first-class reagent, and the remaining reagents were US Sigma, Promega, Qiagen. ) We used products and special reagents.

In other words, 5 g of ethanol was added to 500 g of wasabi powder, which was repeatedly extracted three times with ultrasonic waves for 30 minutes, and then the filtrate was obtained by using a Toyo filter No. 2, and decompressed at 50 ° C. with a rotary vacuum evaporator. The concentrate was freeze-dried to obtain a light green horseradish dry powder. The sample was then used while stored at -70 ° C.

Experimental Example 1 Measurement of Quinone Reductase Inducing Activity of Horseradish Extract Using Mouse Hepatocytes

Quinone reductase (E.C. 1.6.99.2) deactivation assay was measured by Prochaska H.J. and Santamaria A.B. 1988. Anal. Biochem. 169 :: 328-336, 1988, et al.

First, the same number (1.0 × 10 ⁴ cells / ml) of mouse liver cells (Hepa 1c1c7 cells) were dispensed into each well of a 24 well plate, and cultured 24 hours before After adding wasabi extract obtained through, and incubated again for 24 hours, the medium was removed. Then, 250 μl of Lysis buffer (lysis buffer (10 mM Tris-Cl, pH 8.0; 140 mM NaCl; 15 mM MgCl ₂ ; 0.5% NP-40)) was added to each well, followed by Cells were lysed with gentle shaking for 10 minutes under temperature conditions. To this, 1 ml of enzyme solution was added and allowed to react for 5 minutes, followed by 250 µl of reaction stopping solution [0.3 mM dicoumarol; 0.5% pyridine; 5 mM potassium phosphate (pH 7.4)], the enzyme reaction was stopped, and the absorbance at 610 nm was measured.

In addition, the amount of protein was quantified by crystal violet staining method for the same set of well plates. At this time, the enzyme solution is the final concentration of the reaction solution 10 mM Tris buffer (Tris-Cl, pH 7.4), 0.5 mg / ml bovine serum albumin (BSA), 0.008% Tween-20, 40 μM flavin adenine dinucleotide (FAD), 0.8 mM glucose 6-phosphate, 2 U / ml glucose-6-phosphate dehydrogenase, 25 μM nicotinamide adenine Dinucleotide (in NA), 40 μg / ml MTT (3- (4,5-dimethyl-thiazo-2-yl) -2,5-diphenyltetrazoliumbromide) and 1 mM menadione Was used.

Equation 1 was used to calculate the quinone reductase activity, the results are shown in Figure 1 attached.

According to Figure 1, after treatment with mouse green horseradish extract at 10, 25, 50, 100 ㎍ / ml concentration of hepatic cells (Hepa 1c1c7 cells) of the mouse, and examined the induction activity of quinone reductase, wasabi As the concentration of the extract was increased, the induction activity of the quinone reductase increased in a concentration-dependent manner, and the concentration of 100 ㎍ / ml was 2.33 times higher than that of the control without the wasabi extract.

The above results showed that the induction activity of quinone reductase almost similar to 2 μM of beta-naphthoflavone [β-naphthoflavone (BNF]], which is known to have the induction activity of quinone reductase, was found. Considering that 10 μM of aryl isothiocyanate, which is known as a major component of, increased the induction activity of quinone reductase by 1.85 times, it was confirmed that horseradish extract of the present invention has a considerably high induction activity.

Experimental Example 2 Measurement of Inhibitory Activity of Quinone Reductase Using Splog Dooli Rats

Sprague-Dawley rats were tested with gold extracts divided into 5 groups, including the normal group, with dosages of 0, 200, 500 and 1000 mg / kg, respectively. Oral administration over the week. Then, the liver of the mouse was taken to be homogenized with homogenizing buffer (homogenizing buffer (0.25 M sucrose, pH 7.4)), centrifuged at 9,000 xg for 20 minutes (4 ° C), and the supernatant was taken again, at 100,000 xg for 1 hour. The supernatant obtained by ultracentrifugation (4 ° C) was used for the measurement of quinone reductase induction effect. When quinone reductase activity was measured using the same method as Experimental Example 1, quinone reductase activity was increased by wasabi extract in the same manner as in mouse liver cells (Hepa 1c1c7 cells).

This indicates that quinone reductase activity is induced by horseradish extract not only in mouse hepatic cell system (Hepa 1c1c7 cell culture system) but also in vivo system.

In addition, as a result of measuring the body weight after oral administration for 2 weeks, no significant difference was observed between the normal group and the horseradish extract administration group. It did not cause a change compared to the group.

In addition, blood glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) activities, which are indicators of hepatotoxicity, were prepared using conventional methods. The measured results did not show any significant difference compared to the normal group.

Experimental Example 3 Induction Effect of Quinone Reductase mRNA Expression of Wasabi Extract

Total RNA was allowed to lyse cells by adding TRI reagent, and then allowed to stand at room temperature for 5 minutes. 50 μl of chloroform was added thereto, shaken vigorously for 15 seconds, and then left at room temperature for 10 minutes, which was centrifuged at 12,000 × g for 15 minutes to carefully recover the colorless supernatant. 0.5 ml of isopropanol was added thereto and sufficiently mixed. The mixture was left at room temperature for 10 minutes, and centrifuged at 12,000 xg for 10 minutes to obtain RNA pellets.

0.5 ml of 70% ethanol (ethanol) was added to the RNA pellet, and the solution was shaken vigorously, followed by centrifugation at 12,000 xg for 5 minutes to remove the supernatant, followed by drying for 5 minutes in a hood, followed by nuclease. Dissolved in 40 μl of distilled water not contained, heat shock was applied at 70 ° C. for 5 minutes, followed by quantification, and 1 μg / μl was used for reverse transcription test.

Reverse transcription was performed with 20 μl of 5 mM MgCl ₂ , 10 mM Tris-HCl, pH 9.0, 50 mM KCl, 0.1% Triton X-100, 1 mM dNTP, 1 unit / μl of recombinant RNasin ribonuclease inhibitor. inhibitor), 15 units / μg AMV reverse transcriptase and 0.5 μg oligo (dT) 15 primer were reverse transcribed at 42 ° C for 60 minutes.

DNA fragments expected to be the sense and antisense primers used to amplify the quinone reductase and the beta-actin gene, the housekeeping gene, respectively. 290, 591 bp was carried out as follows.

The PCR mix was 50 μl with Taq PCR Master Mix Kit, 2 μl reverse transcriptase reaction product and 20 pmol primer pairs.

The PCR reaction conditions were denatured for 1 minute at 94 ° C., and then cycles of 25 to 30 cycles were performed for 30 seconds at 94 ° C., 45 seconds at 55 ° C., and 60 seconds at 72 ° C. for each cycle. The amplified PCR product was isolated by 2% agarose gel electrophoresis method, stained with SYBR Green I, and confirmed by using an α-Imager. Indicated.

As shown in FIG. 2, 6 μM of beta-naphthoflavone [BNF (β-naphthoflavone)] was treated for 6 hours in the same manner as the quinone reductase activity measurement as a positive control in mouse hepatic cell line (Hepa 1c1c7 cells). It was observed that the mRNA level was strongly increased, the horseradish extract treatment group began to increase the mRNA level from the 25 ㎍ / ml treatment, the stronger band (100) was observed in the 100 ㎍ / ml treatment.

From the above results, it can be seen that the increase in quinone reductase-induced activity by wasabi extract is regulated in the transcriptional stage of the gene.

Experimental Example 3: Toxicity Test

1) Oral Administration of Rats Acute Toxicity

Acute toxicity test was performed using 6-week-old SPF SD rats. Horseradish extract was suspended in 0.5% methylcellulose solution in two animals per group and administered orally at a dose of 1 g / kg / 1 ml. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed. Necropsy was performed to observe abdominal and thoracic organ abnormalities. As a result, there were no clinical symptoms or deaths in all animals treated with the test substance, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc. As a result, all of the tested compounds did not show toxic changes up to 500 mg / kg in rats, and the oral minimum dose (LD50) was determined to be a safe substance of 500 mg / kg or more.

2) Parenteral administration toxicity test for rats

Using the same rat as the oral administration toxicity test, wasabi was administered by the muscle injection method 2 mg each. One rat was administered twice at two week intervals, and the other rat was administered twice at two week intervals, followed by one month intervals, and then again three doses at two week intervals.

Six months after the first injection, the clinical signs, body weight, body temperature, physical examination, blood cell abnormalities (haematology) and fecal abnormalities (urinalysis) were observed, but all showed normal values.

Example 1 Preparation of Chewing Gum (A)

20% by weight of gum base

Sugar 76.5 wt%

Wasabi extract (manufacturing example) 0.5 wt%

1% by weight of fruit flavor

2 wt% water

Chewing gum was prepared according to the above composition and content.

Example 2 Preparation of Chewing Gum (B)

25% by weight of gum base

Sorbitol 72 wt%

Wasabi extract (manufacturing example) 0.5 wt%

Peppermint Flavor 2.5% by weight

Chewing gum was prepared according to the above composition and content.

Example 3 Preparation of Candy (A)

54.5 wt% sugar

45 wt% starch syrup

Horseradish extract (Example) 0.4 wt%

Orange flavor 0.1 wt%

Candy was prepared by the conventional method and the composition and content.

Example  4: Preparation of Candy (B)

Maltitol 53.6 wt%

45 wt% of reduced syrup

Horseradish extract (example) 0.5 wt%

Natural Herbal Flavor 0.14% by weight

Candy was prepared by the conventional method and the composition and content.

Example 5 Preparation of Biscuits

88 kg of flour

Gravity First Grade Wheat Flour 76.4 kg

16.5 kg per white

2.5 kg of salt

2.7 kg of glucose

40.5 kg of farm shortening

5.3 kg of ammo

Medium kg 0.6 kg

0.55 kg sodium bisulfite

5.0 kg of rice flour

0.003 kg of vitamin B ₁

0.003 kg of vitamin B ₂

Milk Flavor 0.16 kg

71.1 kg of water

Whole milk powder 4 kg

1 kg of substitute powdered milk

0.1 kg of calcium phosphate

1 kg of spray salt

25 kg of spray oil

Wasabi extract (manufacturing example) 1.7 kg

The biscuits were prepared by the conventional method and the composition and content.

Example 6 Preparation of Ice Cream

Milk fat 10.0 wt%

Nonfat milk solids 10.8 wt%

12.0 wt% sugar

Starch syrup 3.0 wt%

Emulsifying stabilizer (span) 0.5 wt%

Spice (Strawberry) 0.15 wt%

63.05% by weight of water

Wasabi extract (manufacturing example) 0.5 wt%

Ice cream was prepared by the conventional method and the composition and content.

Example 7 Preparation of Beverages

522 mg of honey

Chioctosanamide 5 mg

Nicotinic Acid 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Wasabi Extract (Preparation) 1,003 mg

200 ml of water

The beverage was prepared by the conventional method and the composition and content.

Example 8 Preparation of Chocolate

Sugar 34.5 wt%

Cocoa Butter 34%

15% by weight of cocoa mass

Cocoa Powder 15% by weight

Lecithin 0.5% by weight

0.5% by weight of vanilla

Wasabi extract (manufacturing example) 0.5 wt%

Chocolate was prepared by the conventional method and the composition and content.

Formulation Example

As confirmed above, wasabi extract has a quinone reductase inducing activity and thus can be formulated into a composition for preventing and treating cancer.

1) Preparation of Syrup

Syrup containing 0.5% (weight / volume) of wasabi extract of the present invention was prepared by the following method.

2 g of wasabi extract and 25.4 g of sugar were dissolved in 80 g of warm water. After the solution was cooled, a solution consisting of 8.0 g of glycerin, 0.8 g of saccharin, 0.04 g of flavor, 4.0 g of ethanol, 0.4 g of sorbic acid, and distilled water was prepared and mixed. Water was added to this mixture to 100 ml.

2) Preparation of Tablets

A tablet containing 15 mg of active ingredient was prepared by the following method.

250 g wasabi extract was mixed with 175.9 g lactose, 180 g potato starch and 32 g colloidal silicic acid. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. It was dried and the mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into a tablet.

3) Preparation of Injection Solution

Injection solution containing 10 mg of the active ingredient was prepared by the following method.

1 g of wasabi extract, 0.6 g of sodium chloride and 0.1 g of ascorbic acid were dissolved in distilled water to make 100 ml. The solution was bottled and sterilized by heating at 20 ° C. for 30 minutes.

As described above, the present invention was confirmed that horseradish extract induces the activity of quinone reductase (Quinone reductase) and QR mRNA expression.

According to the present invention, horseradish extract can be formulated and used in various ways as a composition for preventing and treating cancer. A wide range of adolescents, adults and seniors can be easily consumed anytime, anywhere, so there is an effect of expecting continuous drinking effects.

Claims (3)

Cancer prevention and treatment composition comprising horseradish extract. Health foods comprising horseradish extract and inducing the activity of reductase (Quinone reductase). The health food of claim 2, wherein the food is selected from chewing gum, candy, biscuits, ice cream, beverages, and chocolate.

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