KR20050106894A - Insect natual extract and method for producing the same - Google Patents

Abstract

The present invention relates to a natural extract having a pesticidal, bactericidal and insect repellent action, and a method for producing the same, and more particularly, extracts extracts from natural substances such as ginkgo biloba, baekbu, Bupyeongcho, Seokchangpo and using them as insecticides. It is harmless to livestock, but it can be used for various purposes such as various crops, fruit trees, greenhouses, potted soil, etc.Insects are characterized by mixing ginkgo leaves, baekbu, Bupyeongcho and Seokchangpo at a certain ratio, extracting them, and then decompressing them. It is a natural extract with bactericidal and pest repellent action.

Description

Natural extract having insecticidal, bactericidal and insect repellent action and its manufacturing method. {INSECT NATUAL EXTRACT AND METHOD FOR PRODUCING THE SAME}

The present invention relates to a natural extract having a pesticidal, bactericidal and insect repellent action, and a method for producing the same, and more particularly, extracts extracts from natural substances such as ginkgo biloba, baekbu, Bupyeongcho, Seokchangpo and using them as insecticides. It is harmless to livestock and can be used for various purposes such as crops, fruit crops, greenhouses, pollen soil improvement.

Pesticides have contributed greatly to the increase in agricultural productivity and long-term storage of agricultural products, and their use is steadily increasing. Pesticides currently on the market are chemically synthesized products, which are mainly composed of organic chlorine and organic phosphates. As a result of storage pests, the number of spraying drugs and the increase in dilution concentrations have increased pest control effects. Increasingly, the emergence of new pests is increasing.

In addition, due to excessive use of pesticides, the amount of pesticide residues of agricultural products is increasing, and soil pollution, ecosystem disturbance, and damage to human body from environmental hormones are increasing day by day.

Moreover, the toxic components of currently used pesticides are poorly decomposed and accumulate in the soil or remain in crops, which are mixed with human and livestock food, causing more serious problems for the ecosystem, and many researchers Pesticides are known to cause various cancers and are known to pose serious risks to the environmental aspects and safety of agricultural products. However, the use of pesticides is continuously increasing because there is no natural agent to replace pesticides.

In order to solve this problem, Korean Patent Registration No. 273177, which was previously filed, proposed a method of using ginkgo biloba leaves as an organic pesticide by treating the extract with activated carbon using an aqueous alcohol solution and a nonpolar solvent, but the process such as activated carbon treatment is complicated and It was difficult to commercialize the ingredients after they were produced in a certain fraction, and another patent application No. 415107 was extracted from water with ginkgo biloba or with alcohol and heptane and used as a pesticide such as antibacterial, fungicide or insect repellent. Although not at all harmful to ginkgo biloba leaves alone, insects, insect repellents, repellent (mold), such as mildew had a problem that the effect as a pesticide is inevitable.

Therefore, an object of the present invention is to extract the extract from natural substances such as ginkgo biloba, baekbu, Bupyeongcho, Seokchangpo, etc. in order to solve the problems of the conventional pesticides, crops inhabiting or growing in the soil by using it as an insecticide, a soil improving agent, etc. Natural extracts having insecticidal, sterilizing and insect repelling action to protect crops from pests damaging crops by parasitic leaves, stems, and roots, and to produce pesticides harmless to humans and livestock. In providing.

The natural extract having insecticidal, sterilizing and insect repellent action of the present invention for achieving the above object is characterized in that the ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo are mixed in a constant ratio, and extracted and concentrated under reduced pressure,

The manufacturing method includes the steps of mixing the ginkgo biloba, baekbu, Bupyeongcho and changchangpo in a constant ratio, and the step of adding an extracting solvent 1: 100 to 150 by weight to the mixed mixture; Extracting and filtering the mixture into which the solvent is added; Characterized in that it comprises a step of concentrating the extracted extract under reduced pressure.

Hereinafter, the present invention will be described in more detail.

The natural extract of the present invention is to extract the extract of ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo, and to prepare the extract of the present invention can be applied to a variety of extraction methods, each of the natural substances ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo The composition ratio of can be used to adjust according to the purpose of the extract.

The ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo are mixed and extracted under reduced pressure for ease of use. At this time, the decompression concentration method is also not limited and can be applied to various concentration methods, it is also possible to use the extract immediately without concentration.

Ie the ginkgo leaves. Extracts from Baekbu, Bupyeongcho and Seokchangpo have insecticidal, bactericidal, and insect repellent action, which can be used to combat insects such as ladybugs, leaf horse moths, flyworms, nematodes, caviar, stink bugs, and moths. It can be.

The preparation method of the extract is as follows.

First, ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo are mixed at an appropriate ratio according to the required purpose.

After mixing the natural product in a predetermined ratio, the extraction solvent is added to the mixed mixture in a ratio of 1: 100 to 150, but if the extraction solvent is less than 100 weight ratio, the extraction solvent is insufficient, the extract is not easily extracted, exceeds 150 weight ratio When the concentration is excessive energy is consumed, the ratio is 1: 100 to 150.

At this time, the extraction solvent is methyl alcohol (ethyl alcohol), ethyl alcohol (ethyl alcohol), enbutyl alcohol (n-buthyl alcohol), propyl alcohol (propyl alcohol), ethyl acetate (ethyl acetate), n-heptane (n-heptane) And one selected from the group consisting of purified water and two or more selected mixed solvents may be used, and the solvent is not limited thereto. The solvent is preferably selected according to the purpose of the extract.

For example, when the extract of the present invention is used for the purpose of an antimicrobial agent or a fruit preservative, it is preferable to select purified water as an extraction solvent so that it can be easily dissolved in water, and when used as an insecticide or a repellent, alcohol and en- It is preferable to increase the extraction content of the pesticidal component by using a mixed solvent in which heptane is mixed.

After the extraction solvent is added, it is extracted, filtered, and concentrated under reduced pressure.

At this time, the extraction is not limited to the extraction method, but is most preferably made for 3 to 7 hours at 70 ~ 90 ℃, extraction is not easy if the extraction temperature is less than 70 ℃, solvent is easily evaporated if it is above 100 ℃ If the extraction time is less than 3 hours, the active ingredient is not fully extracted and no more active ingredient is extracted even if it exceeds 7 hours, so it is extracted for 3 to 7 hours at 70 to 90 ℃.

In addition, the reduced pressure concentration also does not limit the method or pressure, generally distilled under reduced pressure between 0.8 × 10 ³ Pa ~ 1.0 × 10 ² Pa.

The concentrated extract may be dried and powdered after the reduced pressure concentration step, and the powder may be mixed with an excipient, a synthetic fungicide or an insecticide, and processed to be used as an excipient, fungicide or insecticide as a powder component.

In addition, it is also possible to process the extract itself by mixing with the raw material for preventing the closet mold, moth insect repellent or grain bedbug production without drying the reduced-pressure concentrated extract.

That is, the extract and concentrated under reduced pressure can be used in a variety of insecticides, fungicides, insecticide repellents, soil improvers, as well as acaricides, anti-corruption and antimicrobial coatings, fruit preservatives, etc. The method of use is also diluted with water It can be used or mixed with other active ingredients, excipients, etc. It can also be used by changing the formulation by powdering, pelletizing, tableting, etc. Unlike natural pesticides that require a small amount in the application amount It can also be used in a large amount by being formulated in, can be used in various ways depending on the purpose of use, application field, and the like.

Hereinafter, the natural products used in the present invention will be described.

Although ginkgo has a difference in content according to ginkgo biloba, root, ginkgo skin, etc., ginkgo biloba contains active ingredients such as glycosides, ginkonic acid, ginkgo gettin, ginkgoides A, B, C, M, and tefenlactone. The structural formula of each active ingredient is as follows.

Centaur (Stemona Japonica, Miq) contains various alkaloids, including stemomine, stemomidine, prostemoni-dine, isostemomidine, tuberostemonidine, hodorine, sessilistemoine, fat 0.48%, protein 9.25%, inorganic salt 12.1 In addition to acetic acid (acetic acid), formic acid (formic acid), malic acid (malic acid), succinic acid (succinic acid), oxalic acid (oxalic acid) is contained, the structural formula of the active ingredient is as follows.

Spirodela polyrrhiza schleid contains calcium acetate, calcium chloride, iodine, bromine, vitamins B ₁ , B ₂ , C, etc. It contains flavones, D-apiose, sterol, protein, tannin, etc., and the structural formula is as follows.

Acous gramineus soland contains 0.11 to 0.42% of essential oils, and β-asarone 63.2 to 81.2%, asarone (trans0 8.8 to 13.7%, Caryophlene, α-humulene, sekishone (1-aryl-2,4) , 5-trimethoxybenzene), trans-4-prophenyl-veratrol and other amino acids, organic acids and sugars.

Hereinafter, the present invention will be described through examples.

(Example 1)

Ginkgo biloba, Seokchangpo, Bupyeong, and White parts were mixed at a ratio of 3: 1: 1: 1, and 120 times of a mixed solvent of methyl alcohol and en-heptane was added to the mixture. Concentrate to prepare a sample.

(Comparative Example 1)

Only ginkgo biloba was prepared in the same manner as in Example 1.

(Comparative Example 2)

Seokchangpo, Bupyeong, Baekbu were mixed at a ratio of 1: 1: 1, and samples were prepared in the same manner as in Example 1.

As an example and comparative example prepared as described above, mold antibacterial activity test, soil cultivation test, leaf horse moth, blueworm, stink bug was carried out.

First, the fungal antibacterial activity test, Pseudomonas Corrugata KACC 10141 and Psedomonas Syringae PV.lachryamans KACC 10396 strains were transplanted to the LB plate and incubated for 24 hours, and each sample was inoculated again in the medium to which each concentration was added and cultured for 24 hours. It was. In addition, the culture medium was properly diluted, transplanted into LB plates, counted after 24 hours, and antimicrobial activity was compared with Ampicillin as a control.

Tables 1, 2, and 3 below show the results of antimicrobial activity against the strains of KACC 10141 and KACC 10396 of Examples 1, 2, and 3.

Result of antimicrobial activity of Example 1 Example 1 (mg / ml) KACC 10141 (CFU / ml) KACC 10369 (CFU / ml) 50 1.0 × 10 ⁴ 1.1 × 10 ⁴ 25 3.8 × 10 ⁵ 4.5 × 10 ⁵ 12.5 6.5 × 10 ⁶ 1.2 × 10 ⁶ 6.25 7.2 × 10 ⁶ 3.7 × 10 ⁶ 3.125 7.9 × 10 ⁶ 8.8 × 10 ⁶ 1.5625 7.9 × 10 ⁶ 1.2 × 10 ⁷ 0.78125 8.0 × 10 ⁶ 5.1 × 10 ⁷ 0 8.1 × 10 ⁶ 5.2 × 10 ⁷ Ampicillin (mg / ml) KACC 10141 (CFU / ml) KACC 10369 (CFU / ml) 0.2 0 - 0.02 0 - 0.002 3.2 × 10 ⁶ -

Result of antimicrobial activity of Comparative Example 1 Example 1 (mg / ml) KACC 10141 (CFU / ml) KACC 10369 (CFU / ml) 50 0 0 25 5.2 × 10 ³ 5.2 × 10 ⁴ 12.5 4.5 × 10 ⁶ 6.5 × 10 ⁵ 6.25 5.7 × 10 ⁶ 1.7 × 10 ⁶ 3.125 6.7 × 10 ⁶ 3.8 × 10 ⁶ 1.5625 7.2 × 10 ⁶ 6.8 × 10 ⁶ 0.78125 7.5 × 10 ⁶ 7.5 × 10 ⁶ 0 8.1 × 10 ⁶ 5.2 × 10 ⁷ Ampicillin (mg / ml) KACC 10141 (CFU / ml) KACC 10369 (CFU / ml) 0.5 0 - 0.2 0 - 0.02 1.8 × 10 ⁴ -

Result of antimicrobial activity of Comparative Example 2 Example 1 (mg / ml) KACC 10141 (CFU / ml) KACC 10369 (CFU / ml) 50 0 0 25 0 0 12.5 0 0 6.25 0 0 3.125 5.0 × 10 ² 1.6 × 10 ⁴ 1.5625 4.0 × 10 ² 9.9 × 10 ⁶ 0.78125 1.4 × 10 ⁴ 1.9 × 10 ⁷ 0 7.1 × 10 ⁵ 2.0 × 10 ⁷ Ampicillin (mg / ml) KACC 10141 (CFU / ml) KACC 10369 (CFU / ml) 0.5 0 0 0.2 0 0 0.02 1.1 × 10 ⁴ 2.3 × 10 ⁴

As can be seen through Tables 1, 2, and 3, it was found that the antimicrobial activity of Example 1 is more excellent.

Soil cultivation experiment, to investigate the conditions of the parasitic macronematodes, nematodes, caucus, and beetle parasitic in the soil Example 10 in a granule (35% extract) after spraying 200g to 8㎡ 10cm deep Dig into the soil, mix well, divide in two, half of Chinese cabbage and half of radish. As a result of observation for 40 days, no growth of pests such as castfish and slugs was found, and the second experiment was conducted in the same manner, but similarly, there was no damage of casters, slugs, and constellations.

Experiments on leaf moths, larvae and stink bugs were carried out on the surface of the leaves and mixed with water at a ratio of 2: 8 in the cabbage and radish growing in the first time, and sprayed on the surface of the leaves every 5 days and observed for 1 month. However, no damage of pests such as stink bugs and blue worms was found.

Through the above examples it can be seen that the extract of the present invention is excellent in effect as insecticide, sterilization, insecticide repellent.

As described above, although the present invention has been described with reference to the above embodiments, it is not necessarily limited thereto, and various modifications may be made without departing from the scope and spirit of the present invention.

As apparent from the above description, according to the natural extract having the insecticidal, bactericidal and pest repelling action of the present invention and a method for preparing the same, the extract is extracted from natural substances such as ginkgo biloba, baekbu, bupyeongcho, and spearmint, and is used as a pesticide, a soil improving agent, and the like. It can be used to protect crops from pests that damage crops by inhabiting the leaves, stems, and roots of crops inhabiting or growing in soil, and to produce pesticides that are harmless to humans and livestock. to provide.

In addition, non-toxic, easy-to-handle, eco-friendly natural materials can be used as substitutes for pesticides, preventing environmental pollution and solving pesticide residues in the crop. Because it can be manufactured by processing, it provides the effect of increasing the profits of farmers and of increasing the export of ginseng, fruits, vegetables, etc., which are problematic due to residual pesticides.

Claims (6)

Mixing the ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo in a constant ratio, and injecting the extraction solvent into the mixed mixture in a ratio of 1: 100 to 150 by weight; Extracting and filtering the mixture into which the solvent is added; Method of producing a natural extract having a pesticidal, sterilizing and insect repellent action, characterized in that it comprises the step of concentrating the extracted extract under reduced pressure. The method of claim 1, The extraction solvent is one or two or more mixed solvents selected from the group consisting of methyl alcohol, ethyl alcohol, enbutyl alcohol, propyl alcohol, ethyl acetate, en-heptane and purified water insecticide, sterilization and pest avoiding action Method of producing a natural extract having. The method of claim 1, The extract is a method for producing a natural extract having insecticidal, sterilizing and insect repellent action, characterized in that made for 3 to 7 hours at 70-100 ℃. The method according to any one of claims 1 to 3, The concentrated extract is dried and powdered after the reduced pressure concentration step, and the powder is mixed with an excipient, a synthetic fungicide or an insecticide, and further comprising the step of processing the natural extract having insecticidal, sterilizing and insect repellent action. Manufacturing method. The method according to any one of claims 1 to 3, Method of producing a natural extract having a pesticidal, sterilizing and insect repellent action, characterized in that it further comprises the step of mixing the concentrated extract after the decompression concentration step with a raw material for preventing the production of wardrobe mold, insect repellent insects or grain bedbugs . Ginkgo biloba, baekbu, Bupyeongcho and Seokchangpo mixed with a certain ratio, and extracting it, and then extract the concentrated, characterized in that the natural extract having a pesticidal, sterilizing and insect repellent action.