KR20050090593A - Method to produce media for agaricus bisporus and method for cultivation of agaricusbisporus thereof - Google Patents

Abstract

       The present invention uses waste cotton as a main material, ginseng and rice bran as subsidiary materials, and as an additive, anhydrous gypsum, lime, ganban stone and germanium stone, which contains trace elements such as nitrogen fertilizer, fertilizer, etc. And it relates to a method for producing a high-quality mushroom mushroom compost medium fermented by activating the ecological ring of the microbial system using seawater or a small amount of salt, and a method of cultivating the mushrooms using the same.

Description

Method for producing media for cultivating mushroom mushrooms and method for cultivating sterile mushrooms using the same {Method to produce media for Agaricus bisporus and method for cultivation of Agaricusbisporus etc}

The present invention relates to a method for producing a culture medium for cultivating mushrooms, and more specifically, 4,000 parts by weight of waste noodles as a main material, 240 to 250 parts by weight of rice bran and 500 to 1000 parts by weight of additives, and 100 parts by weight of nitrogen fertilizer as additives. 50 parts by weight of complex fertilizers containing a large amount of urea, such as, gal, goto, phosphoric acid, minerals such as inorganic calcium, anhydrous gypsum, lime, elvan, germanium and seawater or salt It relates to an organic production method of high-quality mushroom mushroom compost medium fermented by activating the ecological ring, and a method for cultivating mushrooms using the same.

Mushrooms have been widely edible to mankind since ancient times by their distinctive flavor and high nutritional value, and are considered to be important in modern times as the third food given to mankind. In the past, it has been known to be effective in simply anti-cancer effect and strengthening immunity of human body, but as the special ingredients of mushrooms are understood quantitatively, all countries in the world, including Japan and China, as well as Japan and China, are grown and cultivated at the national level. The demand for the preservation and protection of the genetic characteristics of the liver is being expanded. Therefore, a lot of technologies for producing these mushrooms in large quantities are being developed around the world, and in the case of Korean mushrooms, many efforts to develop the production technology are mainly focused on growers.

On the other hand, the history of cultivation of Korean Yangsong was first started in Buyeo province in accordance with the economic development policy of the 1960s, and has a cultivation history of about 40 years, which is more reminiscent of more than 200 years of Western cultivation history. Much research and effort is required. Moreover, compared to Gumi, which boasts modern state-of-the-art facilities and produces high-quality mushrooms in large quantities, the grievances of Korean farmers who grow mushrooms are very small and very small.

More unfortunately, all the books and scholarly research on cultivation technology, which has been a guide for all mushroom cultivation farmers in our country, have reaped no progress at the level of the '60s, when cultivation techniques were still first learned from the West. As a result, they are often unable to escape from irrational technological systems that have not escaped from the wrong conventional cultivation methods.

At present, all farming cultivators in our country recognize sterilization or peak heating process as an absolutely necessary process, paying enormous costs for sterilization or alignment, and purchasing and installing special sterilizers for steam boiler or mushroom cultivation. Doing. In addition, steam boilers and sterilizers are operated to consume enormous amounts of heating oil in order to maintain a volumetric space (about 12,000㎥) based on 60 pyeong planters for a minimum of 6 hours to 12 hours at a temperature of 60 to 70 ° C.

Considering the physical properties of rice straw, which is currently used as a main ingredient for culture of mushroom mushrooms in Korea, heating is a method that accelerates the fermentation process to satisfy the nutritional requirements of mushroom mushrooms due to the lack of mechanization. Is essentially understood.

On the other hand, in terms of the recycling of resources and the productive use of surplus farmer's products, there is also a problem of consuming a huge amount of heating oil due to heating, but cultivating mushroom mushrooms in terms of using rice straw, a farmer product of Korea, It can be understood to be somewhat desirable.

However, the time when Korean farmers cultivated mushroom mushrooms was the time when all the mushrooms produced by canning were processed and exported to the logic of economic development policy in the 1960s. Therefore, the quality of mushrooms produced for processing was not a problem, but production was all a concern for mushroom cultivation, and it was enough to focus only on the stock of production from the viewpoint of mushroom cultivators.

Through this process, as Korea entered the ranks of middle-tier countries, the Korean mushroom industry faced a major crisis such as export cessation due to rising labor costs. However, in order to survive, the Yangshuo planted self-contained by raising domestic consumption through the hard work of farmers. Nowadays, it is necessary to grow high-quality mushrooms that meet the demanding consumption tendencies of consumers.

More important than the current production problem is the superior quality of the mushrooms produced. Compared to this reality, the physicochemical component of rice straw, which is the main material for cultivating mushroom mushrooms, inevitably shows a very serious qualitative degradation compared to the 60s. The current consumption tendency of our people, which cannot be compared to the level of the 60's, demands more nutritious and tasty high quality rice, and all the research efforts on rice farming have improved the seed to produce more nutritious and flavorful rice. With the development of biotechnology, all the nutritive value produced in rice is developed cultivated seeds concentrated in the fruitful rice, minimizing the nutritive value remaining in the by-product, and cultivation of hydrangea using rice straw, which is almost nutrient remaining. to be.

In addition, as a result of the development of rice varieties that are resistant to cultivation, ash is the only ingredient added to all rice straws. The excess of these ashes is absolutely detrimental to the microorganisms involved in fermentation for mushroom culture. In addition, the current government is compensating for the fallow response with the policy of reducing rice, and the rice straw shortage is appearing due to the reduction of rice farming. Therefore, the price of rice straw supplied in the market is increasing day by day.

Most of the smallest growers are only a one-time, improvised plan for the development of the medium. Recently, these short-term ideas have been gathered here and there with only excessive trade, and ridiculed farmers with ridiculous propaganda. There is also a lot of work to make a bad badge for the sale of badge materials secured by.

Already claiming that it is an alternative to rice straw, which is already being adversely imprinted as the main ingredient of mushroom culture, the method of producing media using the material still follows the technical level of the 60s and uses steam boilers and sterilizers. The economic loss of such failures is that the farmers must carry their own stead.

The emergence of raw materials that contain important technical problems that have been missed in the production or cultivation of mushroom straw cultured using straw is inevitably worse than that of rice straw, and in fact it is a deterrent to the development of the Korean mushroom industry. It is working. The technical challenges facing the Korean mushroom industry today are not only the problems of the main materials that are currently used industrially, but also the shifting of the fundamental perception of the grower's entire cultivation as well as the entire process of producing the medium through the entire cultivation. What can be provoked is that it must be developed.

If you are currently using rice straw to manufacture the medium, it is a common trend to double the amount of manure added to supplement the lack of rice straw, but this is due to acidification of the soil due to the use of excessive chemical fertilizers and pesticides. It is inevitable that by supplying the acidified component of rice straw, which is derived from, double the amount of phosphoric acid, which is the most representative component of poultry, will cause extreme phosphate excess in the cultivated compost medium, which will cause fatal loss in cultivation of the fungus. In fact, in recent years, many farms have failed to cultivate the fungus bacteria, ruining the entire cultivation. In addition, in the case of Korean Patent Publication No. 2003-59775, which claims to be an alternative material, the imported cotton by-products that claim to be the main material are subjected to outdoor fermentation for 10 to 15 days as conventional fermentation method of conventional rice straw, and then again using a steam boiler or a sterilizer. It is promoted that sterilization of mushroom seed spawn by sterilizing it for at least 6 hours at 60 ℃ and fermenting it again for 9 days, but the characteristics of microorganisms involved in fermentation of solid material for producing mushroom culture are not known. It is clear that this neglected approach cannot contribute to technological development. In other words, it is easy to understand that the fermentation process is applied to the same fermentation period as that of a relatively hard material such as rice straw without considering physical properties of cotton by-products used as main ingredients. In addition, the materials used in the cultivation technology books of the practice are used as they are.

Fermentation is an organic life activity involving microorganisms, but fermentation cannot occur in large nutrient sources where all bacteria have been sterilized. The microbial group that leads glycolysis is also part of the microbial ecosystem, so it cannot be active for 15 days (360 hours) at temperatures between 50 and 60 ° C. The contents of Korean Patent Laid-Open Publication No. 2003-59775 are sterilized for at least 6 to 12 hours at a temperature of 60 ° C., and then fermented for about 360 hours at a temperature range of 50 ° C. to 60 ° C. again. It is a real phenomenon of time that does not require the sterilization of the microbial phase that should be involved in the fermentation, and does not require the fermentation of the sterile mass again. In addition, the amount of the material that is not suitable for the culture medium (in the case of rice hull added to the cotton batting medium in Korean Patent Publication No. 2003-59775) without any consideration of the component properties or physical properties of the added material, In spite of the fact that the quality of the product is being mixed at random without neglecting quality, only the cases of ignorant farmers are getting worse.

Manufactured and marketed by the bellows sales company of Korean Patent Publication No. 2003-59775, which is considered to be the most representative region of cultivation in Korea, in the Buyeo Boryeong area, which began in the summer of 2003, and is full of propaganda techniques. It is known that no farmhouse uses a mushroom badge, and the damage caused by the mushroom badge purchased by them is a considerable amount.

This is just one unfortunate example of following a methodical number that had to be inevitably adopted due to the nature of rice straw material. The hardest cultivation of the cultivated farmers sighs, and the current level of cultivation of our country's cultivation technology is the biggest problem.

The present inventors first devote their efforts to develop a high-quality medium for the production of mushroom mushrooms using other materials that can be mechanized and provide high-quality organic nutrients. By breaking away from the method categories across the board, understanding the entire cultivation as an organic ecological ring and prioritizing the conceptual transformation of ideas using it, a tricky and incomprehensible process called sterilization and alignment, the most important process in conventional cultivation. Rice straw, which is difficult to work mechanically, omits the most economically wasteful process, and differs in physicochemical properties from waste cotton and essential ingredients such as flour, rice bran, and additives such as nitrogen, garlic, goto, and phosphoric acid. Compound fertilizers containing large amounts of urea The present invention is made by producing a culture medium for growing mushrooms, which can be mechanized by selectively using anhydrous gypsum, lime, elvan, germanium and seawater or salt containing a large amount of trace elements, such as inorganic calcium and active organic microorganisms. Was completed. Therefore, it is an object of the present invention to provide a method for producing a sterilized mushroom-free sterilized medium for cultivating mushrooms and a method for cultivating mushrooms using the same.

The present invention comprises the step of containing 70 to 80% water in 4,000 parts by weight of the cotton; Performing a regression to discharge excess moisture contained in the direction of gravity; After one day, the step of depositing 100 parts by weight of hydrated lime or quicklime in a mixture of 240 to 500 parts by weight of rice bran and 500 to 1000 parts by weight of scale; A first inversion by adding 100 parts by weight of urea fertilizer and 50 parts by weight of the compound fertilizer to enhance microbial activity and release the ammonia gas accumulated in the medium one day after the present deposition; A second inversion by evenly spraying 400 to 800 parts by weight of germanium dissolved water and 5 to 15 parts by weight of elvan powder to promote growth of aerobic microorganisms and inhibit growth of anaerobic microorganisms after one day from the first inversion; Control the calorific value of microorganisms within one day from the second inversion, discharge the ammonia gas emitted by the microorganisms, supply 400-800 parts by weight of seawater or brine and meet the third inversion to meet the vigorous oxygen demand of aerobic microorganisms. ; After the third flip, performing a fourth flip while shaking the medium after the third flip in the air twice a day so that the central temperature of the stack of media does not exceed 60 ° C .; A day after the fourth flipping, evenly spraying anhydrous gypsum 200-250 parts by weight to the fifth flipping; Shaking the medium deposited outdoors to completely discharge residual ammonia gas and carbon dioxide gas and supplying oxygen to cool the temperature of the medium to about 30 ° C .; The cooled medium is laid with 120cm wide and 80cm long heiden vinyl with 1000 ~ 1200 holes perforated with a diameter of 3mm ~ 5mm inside a plastic box, and 15-15 parts of medium is filled, and the contents inside the box are completely covered. Granulation step; The boxes are moved inside the fermentation chamber so that the space between the boxes is 15 ~ 20cm apart from each other.The lowest box is 1.5cm or more from the ground and lamination is performed up to 9 steps, but 3 steps, 4 steps, 6 steps, and 7 steps. Laminating at intervals of 30 cm from each other; Performing a first ventilation when the temperature of the medium reaches 50 to 60 ° C. after 12 to 18 hours after lamination; Closing all doors of the fermentation chamber and waiting 10-12 hours; Performing a second ventilation after opening the door of the fermentation chamber after a lapse of time; Ventilating so that the air in the room may change every 3 hours after the secondary ventilation, and stopping the ventilation when the temperature of the medium naturally reaches 45 ° C; It relates to a method for producing a mushroom mushroom cultivation medium, characterized in that it comprises the step of rapidly lowering the temperature of the medium to 20 ~ 25 ℃ using a cooler after the ventilation stops.

In another aspect, the present invention relates to a cultivation method of mushroom mushroom, characterized in that by cultivating the mushroom mushroom by inoculating by using less than 200 bottles (200 pounds) of the spawn seed spawn on the medium of 60 pyeong of the average bacterium on the post-fermentation finished medium will be.

The present invention will be described in detail by first dividing the whole process of producing the medium for mushroom mushroom cultivation into a first process and a second process, and then divided into a third process of cultivating mushroom mushroom using the prepared medium.

The process for producing a culture medium for mushroom cultivation includes supplying water to 4000 kg of waste noodles to make the waste noodles contain 70 to 80%, preferably about 75% of moisture, and then dropping them; After repulsion, the gravitational water is sufficiently drained, and the first step is to mix 500-1000 kg of rice flour, 240-500 kg of rice bran, 100 kg of slaked lime or quicklime; Mixing the materials evenly and performing a full deposition, and after 24 hours, 100 kg of urea fertilizer and 50 kg of complex fertilizer containing a large amount of urea such as girly, phosphoric acid, and goto are added to perform the first flipping; After the first inversion, turn over every 24 hours until the temperature at the center of the compost pile rises to 65 ° C. In each turn, turn 5 to 15 kg of gantherite powder and 400 to 800 L of germanium dissolved water or 400 to 800 L of seawater. Spraying evenly and evenly mixing and stirring the provided materials for 4 to 7 days to perform outdoor fermentation; After the outdoor fermentation is finished, the acidity of the medium is calibrated using gypsum or calcium carbonate so that the pH is 7.5 to 8, and then the medium temperature is 30 ° C. or less, and then put in a ventilated box to allow the microorganisms to breathe; The box containing the compost is transferred to an indoor post-fermentation room and stacked so that the mycelia of mushroom mushrooms can be used as a nutrient source. Performing ventilation so that the whole can be circulated; Comprising the self-centered temperature of the compost in the stacked box reaches 45 ℃ when all the doors inside the room is ventilated at a time to perform a quenching so that the compost temperature is 20 ℃ or less.

In addition, the present invention provides a cultivation method of mushroom mushroom, characterized by inoculating the mushroom seed spawn in the medium prepared by the above method.

The present invention uses waste cotton, which is a by-product of cotton, as a main material for producing a high-quality mushroom cultivation compost medium. There are some importers that are promoting the by-products of domestic spinning factories, but if you follow the present invention, you can use domestic cotton masks at all, but there are many more advantages, and you can use imported cotton masks. Urea fertilizer as a source of nitrogenous material for using carbonaceous powder and rice bran as subsidiary materials and for adjusting carbon mass as an additive; Complex fertilizers in which a large amount of urea such as phosphoric acid, girly, goto, and boron are properly mixed; Inorganic calcium that meets the essential nutritional requirements of mushrooms; Mineral component; Anhydrous gypsum; Germanium stone; Elvan; And sea water or salt.

The present invention escapes the conventional conventional cultivation method that only mushroom mushrooms can be used and other bacteria should make compost medium unavailable, but rather maximizes the cultivation of other bacteria that mushroom mushrooms can use as essential nutrients.

The present invention is a fermentation process of the composting medium using the ecological ring (feed chain) of the microbial ecosystem with the above-described subsidiary materials, auxiliary materials and additives and the like is different from the fermentation concept of conventional mushrooms composting medium.

<Step 1>

Step 1: save the water

The purpose of the water harvesting operation is to create an ecological environment of microorganisms in the selected main material, to contain about 80% water in 4,000kg of cotton. The method uses a cotton wool or a tractor to finish the work in the shortest possible time. Here, the cotton wool machine uses centrifugal force to rotate the waste surface with water to contain moisture in the waste surface, and the tractor uses the wheel to trample the waste surface with water to make it contain moisture. Other mechanical methods also follow this method in principle, and once the draining operation is finished, the closed surface begins to be called a medium.

Phase 2: Regression Process

The purpose of the retreatment is to stock the moisture-containing media in a state that is easy to manage and able to carry out future process operations smoothly. That is, to discharge gravity water. This can be done mechanically or manually, but it is advantageous to deposit as high as possible in a 5 to 6 meter diameter area using machines such as loaders, tractors and excavators, as the amount and weight of the medium is significant.

Phase 3: Full-Secondary

The main purpose of this step is to attract 240 ~ 500kg of rice bran and 500 ~ 1000kg of poultry from the selected materials to attract microorganisms in the air and draw them in the medium. Although replenishing the oxygen in the lost pile to induce the medium to be aerobic, it is not considered to selectively attract only aerobic flora of the microbial population in the air. In other words, anaerobic flora is also attracted together. The method of this work is carried out using the loader, tractor, excavator, etc., and the deposited medium is sized to about 1.5m in height, 5m in width and 10m in length.

Step 4: flip the first

The main purpose of this work is that after the previous stage, the medium pile begins to cool down (30-45 ° C) after 1 day in summer and 2 days in winter, which causes bacteria attracted into the medium to begin to produce fever. In this step, the essential nutrients required at this time are evenly supplied in the medium, so that the microorganisms are more active, and the ammonia gas that is already accumulating in the medium is released and fresh oxygen is supplied.

In this process, 100kg of urea fertilizer and 50kg of complex fertilizer containing a large amount of urea such as girly, phosphoric acid and goto are mixed in order to supply the selected subsidiary materials evenly, which supplies essential nutrients required by various microorganisms in the medium. It can be said.

Step 5: Flip the Second

The main purpose of this work is to control the balance of the various microbial populations distributed in different parts of the stacked media stacks to selectively culture only the microorganisms capable of synthesizing the nutrients that mushroom mushrooms can use directly. It aims to make it as aerobic as possible, and then kills by exposing the anaerobic microorganisms in the center of the media to the air and making the precursors available to the aerobic flora. The additives supplied in this process are evenly sprayed with 400-800 liters of germanium dissolved water and 5-15 kg of elvan powder.

Step 6: flip the third

The purpose of this work is to control the calorific value of the microorganisms in the media stack, which has already reached a temperature of 50-60 ° C, to release the ammonia gas emitted by the microorganisms in a timely manner and to meet the vigorous oxygen demand of aerobic microorganisms.

In this process, 400 ~ 800L of seawater or brine is supplied to supply various mineral elements required by aerobic microorganisms, which meet the microelement requirements of microorganisms with limiting substrates, thereby significantly increasing the number of generation and It is an essential work process to make the whole into a macro nutrient source that can be used directly by mushroom mushrooms.

Step 7: flip the fourth

This operation is like usually begin to 2 ~ 3 days, activated in this case the surface layer from the medium pile by the point in time at which Streptomyces (Streptomyces), Thermo North Fora (Thermonospora) from the time the mulchuk is working pyemyeon end bangsasanggyun and Pseudomonas ( Pseudomonas ) is to help the growth of non-spore bacteria , such as Humicola ( Humicola ) to induce the dominance of mesophilic filamentous fungi. The method of execution of the work is flipped whilst in the air using the machinery once a day so that the temperature in the center of the discharge pile does not exceed 60 ° C.

At this time, the ammonia gas and the carbon dioxide gas emitted are caused by the growth of anaerobic microorganisms in the medium. Various nutrients accumulated in the medium forming the conversion area of these microorganisms can be used as food for thermophilic microorganisms. Or, by supplying oxygen that is essential to aerobic microorganisms, the microorganisms involved in fermentation can fix the ammonia form nitrogen in their conversion zone with protein so that the finally inoculated mushroom mushroom mycelium can be used as a direct nutrient source. .

Step 8: Flip the 5th

Due to the physiological action of the microorganisms activated by the mixing of all the provided materials, heat is supplied to feed soluble sugars and amino acids, which are nutrient sources that are relatively easy to decompose, and the activity is rapidly increased to decompose the fibers constituting the lungs. Then, while living by using various decomposition sugars and ammonia, he himself accumulates life in the compost and releases a large amount of heat, moisture, and carbon dioxide. Through this process, cellulose and hemicellulose in the already provided materials such as lung cotton are decomposed more than half and consumed as nutrients for fermentation microorganisms, and nitrogen in ammonia is fixed as protein by various microorganisms, while 5-10 It combines with lignin, which accounts for%, to form a lignin protein (a polysaccharide granule complex), which serves as the final nutrient source of mushroom mushroom mycelium.

In order to improve the binding of the medium due to colloidal dispersion, which is inevitably generated during this process, and to provide trace elements such as inorganic calcium required by mushroom mushrooms, anhydrous gypsum 200kg ~ 250kg is evenly sprayed. Car reverse. This is a very important work process that is also used for the purpose of controlling the final moisture content of the medium during the so-called outdoor fermentation process in all processes that have been made up to now.

Here, anhydrous gypsum is suitable for the life cycle of hyphae hyphae to increase the moisture content of the media with excessive water content due to the increase of condensation water produced by combining with hydrogen molecules in the air called by aerobic microorganisms using oxygen as the final electron acceptor. It is a material for adjusting to about 75% water compared to a solid medium, and can be used depending on the current water content of the medium, which means that even if an excessive amount of anhydrous gypsum is not adversely affected by microorganisms.

The work process thus far is collectively referred to as outdoor fermentation in practice, and the present inventors intend to use this as the first process. Because the primary purpose of this first process is not to ferment, but rather to mix all the ingredients provided to the fermentation microorganisms used to make the culture medium, but to achieve basic growth of useful microorganisms attracted in the process. It is intended to collectively refer to this series of work processes in a broader sense than the relatively limited scope of the term fermentation.

On the other hand, the time required for the first process usually takes 5 to 7 days except for mutants such as bad weather changes. After the main deposition, one turn a day is carried out, and each time the mixing of the corresponding materials is performed.

<Step 2>

Stage 9: Winner

Since all the work processes in the first process are usually performed outdoors, the fermentation process of the mushroom mushroom medium, which had to be relatively irregular and coarse, such as varying weather conditions, is more precisely managed, and the synthesis of nutrient sources required by the mushroom mushroom mycelia is required. And a second step (post-fermentation in the conventional name) for enhancing the repellency or defense of the medium from various germs is essential. As a first step, granulation begins with laminating the medium produced in the first process into boxes and transferring them to the indoor fungus.

This work is performed by finely shaking the pile of medium deposited outdoors using centrifugal force of cotton wool to completely discharge the residual ammonia gas and carbon dioxide gas, and supplying enough oxygen, the medium having the center temperature of 60 ~ 65 ℃ is 30 It is quenched by the inside of ℃ ℃, it is divided into plastic boxes of 55cm ~ 60cm wide, 40cm ~ 45cm wide, 15cm ~ 20cm high, which can be transported by manpower, and it is transferred to the fungus installed inside the fermentation chamber or inside the mushroom cultivator. To be laminated on. At this time, the method of dividing the badge into a box is 120cm wide and 80cm long with a hole of 1,000 ~ 1,200 diameters of 3mm ~ 5mm perforated inside a plastic box, and 15kg ~ 20kg of medium is placed inside the box. Cover the contents completely. At this time, covering with perforated hydride vinyl is a measure to prevent the dehydration that is excessively dried by self-heating of the medium due to the structure of the box during the post-fermentation process and to allow the breathing of aerobic microorganisms in the medium. If it is manufactured precisely enough to carry out the above structural functions, this can be done by directly boxing without vinyl coating.

10th step: lamination

The number of boxes made in the above working step is about 1,300 to 1,500 boxes based on the amount of dry cotton as the main material. This is transported into a fermentation chamber using a transport port, and the stacking method is to keep the distance constant so that the space between the boxes is 15 to 20 cm apart from each other. It is a measure to facilitate supply of oxygen for the purpose, and the respiratory heat of beneficial bacteria, which have been well-cultivated in all processes up to now, is accumulated so that the temperature inside the fermentation chamber rises sharply and prevents the temperature of the medium contained in the box from rising more than necessary. This is for.

While the proper ecological temperature of Actinomycetes and other thermophilic flora required is around 50 ~ 60 ℃, if the medium itself temperature rises above 65 ℃, it will be able to live in this zone of 65 ~ 80 ℃. The abnormal proliferation of super-high temperature microorganisms may cause chemical change of the anaerobic state, which is inadequate for mushroom mushroom cultivation medium. Be sure to start stacking by raising more than 1.5m, and in the case of the box of the above size, avoid excessive stacking of three or more stages, and after stacking three boxes, stacked them up at a distance of 30 cm or more, Do not stack at 9 steps, that is, 3 m or more.

Step 11: Primary Ventilation

After 12 to 18 hours have elapsed since the lamination of the box is complete and the granulation work is completed, the center temperature of the medium in the stacked box is usually restored to 50 ~ 60 ℃. In accordance with the second step of the first ventilation.

Primary ventilation should be completed quickly with a blower, etc. with all doors open so that the air in the room can be completely replaced in the shortest possible time. The main purpose of this work is to complete the fermentation process of the medium which has not been completed in the first step by supplying an appropriate amount of oxygen to the stacked medium boxes.

Step 12: Peak Heating

The traditional practice of cultivation is called sterilization. The contents of this work are 600 ~ 1200L of light oil by using expensive equipment such as steam boilers or sterilizers to grow copper on an average floor area of 60 pyeong. It is a very important process that had to be heated to sterilize mushroom mushroom medium in the temperature range of 60 ~ 70 ℃ for at least 8 hours and up to 36 hours. On the other hand, if you compare the cost of each step in cultivating mushroom mushrooms, the cost of this process is the greatest. In addition, this is a very unreasonable process and can be said to be an unreasonable practice that must be corrected for the development of the Korean mushroom mushroom cultivation industry.

Fermentation is the modern production of the metabolite as a product using the physiology of microorganisms. Since the culture of mushroom mushrooms is made by fermentation, sterilization is not an essential process. If you focus on how Pasteurization, or pasteurization, in the temperature range of 60 to 70 ° C, disrupts the ecology of microbial populations involved in fermentation of mushroom mushroom media, and how big the damage is, Economically, the useless sterilization process, which is a poor country with no drop of oil and is an essential part of the process of producing a medium for raising mushrooms, which is personally paid at great expense, must be replaced by economic methods. do.

The invention does not first heat up at all for this working step. Therefore, there is no need to install expensive equipment and there is no consumption of oil such as diesel. In addition, it produces a much more living and organically healthy mushroom mushroom cultivation medium than the sterilized mushroom cultivation medium that is produced at great expense.

When mushroom mushrooms are produced with the mushroom mushroom cultivation medium produced according to the present invention, high-quality mushroom mushrooms are produced that are superior in quantity and quality to those of conventional cultivation. No special preparation is necessary for this work step. However, if all 11 steps have been faithfully implemented, step 12 is to close all doors in the fermentation room and wait 10 to 12 hours. Inside the fermentation chamber, the total amount of exothermic heat generated by the aerobic microorganisms that are organic and activated in the first process is generated and maintained at a room temperature of 50 to 55 ° C. and a medium of 60 to 65 ° C. At this time, it is important to pay attention never to heat. If the room temperature is only 50 ° C, if it is arbitrarily heated to sterilize, as it is used in conventional practice (based on the room temperature of 60 ° C in the conventional method), it will artificially modify the microbial ecology ring formed inside the medium. This results in disturbances and reduces the conversion area of filamentous fungi, a high-quality protein source needed by membrane mushrooms, which not only reduces the amount of precursor produced, but also results in high temperatures unique to filamentous fungi. Due to the collapse of the sex ecological temperature band, there is a risk that synthesis of poor protein sources required by the fungus, ie, mushrooms, will be destroyed and poor protein sources may be produced. The idea that the culture inside the box continued to grow as a giant nutrient The flora kills itself almost instantaneously at the time of their maximum growth, when nutrients corresponding to their essential nutritional requirements are already depleted in the medium, or when their maximum density is reached, or external environmental factors Of actinomycetes or actinomycetes , such as Streptomyces , Thermonospora , and apoptotic bacteria such as Pseudomonas , and mesophilic microorganisms such as Humicola. As the degradation products of thermophilic microorganisms, which may become nutrients, become poor, the proliferation of actinomycetes in the chain becomes poor. This results in a high quality nutrient source, which is essential for the culture of mushroom mushroom cultivation, and a decrease in resistance from various bacteria.

In Actinomycetes flora, microorganisms such as Streptomyces usually have a narrower conversion area and leave their cells as precursors without the production of metabolites. For example, antibiotics are secreted from the precursors of these actinomycetes, which are their bodies, which prevents other fungi from multiplying into their dead cells. In other words, this advantage is used to enhance the resistance of mushroom culture medium to mushrooms.

This is an innovation of the sterilization process, which is considered to be made of meat because of the physical properties of the rice straw, which was the main ingredient of the medium selected in the conventional method of cultivating the culture of conventional mushrooms. Even the irrationality and costly non-economics that had to be sterilized were completely improved, and more than 70 kinds of microorganisms, which are known to symbiize in the growth of mushroom mushrooms, have succeeded in producing organic non-sterile medium.

The medium sterilized by the conventional cultivation method has a large amount of microorganisms of this symbiotic relationship, and therefore, it was inevitable to produce mushrooms with deteriorated quality, and considering that they were exposed to various invasive bacteria without defense, The success of the preparation of this sterile mushroom mushroom medium is indeed worth doubling.

Korean Laid-Open Patent Publication No. 2003-59775 discloses a process for preparing mushroom mushroom medium using bellows and a blending material. The mushroom mushroom containing nicotine is added to the mushroom medium due to the addition of tobacco powder. In addition to the risk of production, papers have already been published in Korea that reveal the detriment of the use of bismuth as an absent material of the medium. It is already known that more than one percent is the same. Phosphoric acid components such as rice straw and cotton by-products, which are physically completely different, are heated in the same manner using a sterilizer, or chicken flour used to soften high-content lignin found in relatively hard farmer products such as rice straw. The compounding ratio and the long fermentation period used by applying the same to soften soft cotton wool are wrong.

Moreover, in the case of chaff mixed in a certain ratio in the bellows medium in the Republic of Korea Patent Publication No. 2003-59775, the rice straw has a softer property more difficult and any microorganisms that decompose the solid medium material is difficult to parasitic The fact that it has been neglected is that it is a pity that it can produce high-quality mushroom mushrooms by mixing it with bellows in a random way, as if it is a sidewalk.

Xylan, which is known as the main component of the higher plant cell wall, is a xylose polysaccharide, widely distributed like cellulose. In particular, straw or cotton contains 5 to 50% of the major component of xylan. Many thermophilic fungi, such as Sacchromonospora sp , degraded xylan in mushroom mushroom media (Deploey and Fergus, 1975; Rosenbeurg, 1978; Trigiano and Fergus, 1979). It is consistent with the fact that xylase degradability is commonly found in the thermophilic fungi that occur, while thermophilic fungi, i.e. filamentous fungus called white fungus, have not been found to degrade lignin. have. This simply indicates that the chaff contained in a considerable proportion in the mushroom culture medium of Korean Patent Laid-Open Publication No. 2003-59775, in which aerobic fermentation is emphasized without the exact basis in the production of the mushroom mushroom medium, is very unreasonable. Giving is evidence.

Aerobic fermentation is a medium dominant phenomenon of thermophilic microorganisms, namely, thermophilic fungi, in which the xylase resolution is found several times in the thermophilic fungal flora, while lignin resolution is found. No matter how aerobic fermentation proceeds, there is no possibility that lignin in the culture medium for mushroom cultivation can be degraded and consumed about 700% more chaff than cotton straw compared to rice straw.

Materials that cannot be decomposed and used cannot be added to culture medium for mushroom mushroom cultivation. In Korean Patent Laid-Open Publication No. 2003-59775, diesel and kerosene used in conventional conventional cultivation using a sterilizer or a steam boiler again. It is a sterilization that consumes generously, but there is a part to understand that it was forced to mobilize steam as a way of promoting softening of relatively hard rice straw, Soft cotton wool that is only soft should not be cooked with a steam boiler. When heated, the thermophilic fungal flora with lignin resolution kills even the pyrogenic fungi with xylan resolution lost their unique life cycle. The method of Korean Patent Publication No. 2003-59775 which started with a propaganda led by an instructor of a mother farming college in Korea in Chungbu, Buyeo, Boryeong, which is known as the main producer of mushroom mushrooms in Korea as of December 2003. All of the farmhouses that used to have disappeared and the false constitution of the ferocious mushroom cultivation medium could be known early.

Step 13: Secondary Ventilation

Open the door of the fermentation chamber after the alignment process and perform the second ventilation. It is a thermophilic compound that synthesizes high-quality nutrients favored by mushroom mushroom mycelium by rapidly eliminating the accumulation of heat generated by exothermic microorganisms and the rapid accumulation of residual carbon dioxide and ammonia gas in the room, followed by aerobic fermentation. The task is to supply fresh oxygen so that microorganisms can multiply to higher densities. The medium temperature at this time is usually 60-65 ℃, but by opening all the doors and ventilating, it is effective to prevent the temperature from exceeding this temperature range naturally. If this secondary ventilation is delayed, the total heat of the exothermic microorganisms will accumulate and reach a higher temperature range, thereby destroying the filamentous fungus that decomposes and synthesizes the high-quality nutrients needed by the mushroom mushroom mycelium. Microorganisms will begin to multiply and become a useless compost pile of anaerobic bacteria concentrated in the medium.

The method of the second ventilation operation is to average the sum of the center temperature of the medium in the box and supply the outside cold temperature to the room to reach 50 ~ 60 ℃, supplying fresh oxygen and lowering the room temperature. To control.

After that, ventilate every 3 hours so that the indoor air is completely changed, based on the volume space volume of 7m × 20m × 5m (normal mushroom cultivator with a normal area of 60 pyeong), but the mushrooms in the stacked boxes As long as the center temperature of the cultivation medium does not exceed 65 ° C. and does not reach the minimum 45 ° C., temperature control is not involved. This is because the required temperature range of actinomycetes and filamentous fungi is at a maximum of 65 ° C and a minimum of 45 ° C. In this temperature range, various organic nutrients can be decomposed and synthesized by alternating killing and proliferation of various microbial floras. . This venting operation begins to naturally decrease the temperature of the medium and continues to 45 ° C.

Step 14: Haon

If regular ventilation is continued after the alignment, the center temperature of the media in the box will begin to drop, usually from the 4th to 5th day. When the temperature starts to drop and the medium temperature reaches 45 ° C, all the doors in the room can be opened or the machine can be cooled down quickly using equipment such as a freezer. The lower temperature proceeds as quickly as possible so that the medium temperature reaches as fast as 20-25 ° C. As a result, two processes of preparing a medium for cultivating mushrooms have been completed. However, in view of the characteristics of the present invention, an advanced cultivation method different from a conventional cultivation method is provided, thereby providing a whole process of sterile cultivation method using closed cotton.

<Third process>

Step 15: Inoculation

Completely cool down the medium to the seedlings, and inoculate the mushroom mushroom spawn. The amount of spawn used at this time may be slightly increased or decreased between mushroom varieties. However, in case of growers with an average area of 60 pyeong, the consumption preference of the metropolitan area is relatively high in the medium produced based on 4,000 kg of dry land, which is the main material of the medium. Uses 150 bottles of high grain 705 varieties (1 pound bottle currently on the market).

According to the conventional cultivation method, the amount of spawn used is 300 to 500 bottles based on the same plant size as the above, and the same amount is also used in the cultivation of mushrooms of the method of Korean Patent Laid-Open Publication No. 2003-59775, which has been recently troubled. However, the medium of the present invention is characterized by organically activated nature of mushroom mushroom mycelium is very fast and the essential nutrients that can further enhance the vitality of the seed inoculated evenly synthesized because by dramatically reducing the amount of spawn used It is a result of savings in the overall cost of cultivation. While the amount of spawn used is less than 50% of conventional cultivation methods, the results show incomparable performance in the period of incubation after inoculation or mushroom harvest after incubation. Inoculation of mushroom mushroom spawn is done by layer inoculation, but in some cases, mixed inoculation is not relevant.

Layer inoculation refers to an inoculation method in which seedlings are sprinkled by dividing the medium deposited on the bacteria into two layers. In other words, divide the medium on top of the fungus into two, first half of which is evenly spread, then sprinkle about 45% of the seed spawn, then cover the rest of the medium again, evenly and then sprinkle the remaining spawn on it. Pressurize to the medium by pressing with a suitable force to finish.

Mixed inoculation method is to sprinkle evenly divided the amount of seed allotted to the medium loaded on the bacterium, and then mix the medium and the spawn evenly and mix like a mixture to finish the flat top.

Step 16: Cultivate

Cover the newspaper immediately with the inoculation complete and spray it with water and adjust the room temperature to 20 ~ 22 ℃ (room temperature may vary slightly between mushroom mushroom varieties, as described here based on 705 varieties). Increase the humidity as much as possible and start culturing and cultivating the medium of inoculated seedling spawn. Hyphae culture of mushroom mushrooms does not require oxygen, and because the mushroom mushrooms use carbon dioxide generated by themselves, indoor ventilation is unnecessary, but the indoor humidity is as high as possible unless the damage of pests is concerned. And conventional straw straw cultivation or culture period of the bellows medium took 15 to 18 days, but the culture period in the medium of the present invention takes more than ± 1 day.

Step 17: cover

After 10 to 11 days from the completion of the inoculation work of the mushroom spawn, the spawn spawn adheres to the culture medium on the top of the fungus and is cultured. The method of applying the cover work is not different from the conventional cultivation method, but by covering the entire medium with vinyl after cover, it is accumulated to fill the carbon-covered inside of the vinyl cover, so as to make faster cover hyphae.

The thickness of the cover is 1 ~ 2cm thicker than the practice of 3 ~ 4cm to increase the total amount of moisture contained in the cover, and the conventional cultivation method contraindicates the watering after cover, but on the medium of the present invention, the surface area is immediately after cover. Watering water of about 200 ~ 300 ℃ based on 60 pyeong planters. At this time, the amount of water to be irrigated should be adjusted according to the moisture content of the soil to be covered. Usually, the moisture content of the soil to be covered is controlled to about 65%.

Step 18: Futo

After 6 ~ 7 days from the end of the cover work, the cover of the hyphae mycelia begins to be discovered completely. At this time, the covered vinyl is removed and futo is applied. The main purpose of the futo work is to induce uniform injury of fungus hyphae on the cover soil. In other words, by covering the mushroom mycelium that begins to rise from the depth of the cover surface with other soil, the uniform mushroom mycelium is injured on the whole germ. This futo work is usually carried out three to four times, and after about seven hours from the end of the last futo work, it proceeds to the next step of weaving.

Stage 19: Foot Bite

After the completion of the futo work, the first watering is carried out to provide a new environment for the cultivated mushroom mycelium to induce a transition to reproductive growth, thereby harvesting high-quality mushrooms.

Watering is based on the cultivation area of 60 pyeong of the average area, about 1,000L of water is usually divided once a day for a total of three days, so that the water content of the cover soil reaches 75%. Simultaneously with the watering, the indoor temperature of 20 ~ 22 ℃ is lowered to 16 ~ 17 ℃, and the indoor ventilation is performed to reduce the concentration of carbon dioxide accumulated in the cultivator to 1200ppm to provide fresh oxygen to the mushroom hyphae. The mushroom mycelium confronted stops the nutrient growth that has been going on until now and switches to the reproductive growth for the production of fruiting bodies that become mushroom mushrooms.

Stage 20: Harvest

After the ventilation and the lower temperature with the first irrigation, the mushrooms are harvested for the first time after 12 to 13 days. As a result of the cultivation method of the present invention, the total cultivation period up to here is 5 to 7 days in the second process. 4 to 5 days in the 3rd process took 30 to 32 days for a total of 39 to 43 days.

This is the total cultivation period of 75 ~ 80 days in the case of rice straw of the conventional cultivation, 62 ~ 70 days of the bellows medium of the above example, the total cultivation period of about 40 to 50 due to the invention of the non-sterile medium for cultivating the mushroom mushroom of the present invention Shortening the percentages has led to innovative developments.

In addition, the quality and yield of harvested mushrooms are excellent in terms of yield, and the effect of the present invention is remarkable when comparing the amount of spawn used and the total required labor and cost with conventional cultivation methods.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited only to the following examples.

Example 1; Cultivation of Mushroom Mushroom according to the Invention

Waste cotton, a by-product remaining in the domestic spinning factory, was used. In the Republic of Korea Patent Publication No. 2003-59775, although the content of the polyester does not directly affect mushroom cultivation, in consideration of the treatment problem of the waste compost after mushroom cultivation was selected as the low cotton content as low as possible. It is known that the content of polyester is about 0.2%, but actually less than this due to strict quality control. Because the price of polyester is much higher than the price of cotton, each spinning company actively seeks the recovery method, and recently a machine for selectively recovering polyester has been invented. You can get it.

4,000kg of cotton was used to make water by using a cotton wool machine, but the water content was increased to 120% or more by increasing the moisture content as much as possible.

After 24 hours from the end of the retirement period, after checking that the gravitational water, the excess water contained in the lungs, was sufficiently drained, the moisture content of the lungs was about 75 to 85%, and then 600 kg of rice flour, 320 kg of rice bran and 100 kg of slaked lime were evenly Mixing was done.

After 24 hours after the main deposition, it was confirmed that the medium temperature reached 40 ° C, and then the first inversion was performed by adding 100 kg of urea fertilizer and 50 kg of complex fertilizer containing a large amount of urea such as girly, phosphoric acid, goto, and boron. did.

24 hours after the first inversion, the center temperature of the media pile reached 50 ° C, and 5 to 15 kg of germanium dissolved water 400-800 L ganthele powder was evenly sprayed, and then the inverting operation was carried out to be evenly mixed. After the second flipping operation, flipping was performed again within 24 hours, and 500L of seawater was evenly sprayed on the stack of media. After the third inversion, the medial pile temperature rises rapidly due to vigorous growth of thermophilic microorganisms, so that the media piles are inverted twice a day in the morning and evening to prevent the media temperature from rising above 60 ℃ and supply fresh oxygen. As a result, ammonia gas generated in the anaerobic fermentation portion in the center of the medium was released into the atmosphere.

After 6 days from the start of the first work, the end of the fermentation was finished, the media pile was divided into the stirrer sequentially and evenly mixed with 250 kg of gypsum. In addition, the medium mixed with gypsum powder in the stirrer was shaken using a cotton wool machine to discharge ammonia gas in the medium itself, and the oxygen was measured after sufficient oxygen was supplied. Without transition to the next step of work.

The badges coming out of the masking machine are placed in a box 45cm long, 60cm high and 20cm high (commonly known as poultry box, made of a net-like structure with a net-like structure that allows all five sides to be fully ventilated). Each piece of perforated 120cm wide and 80cm high heiden vinyl was laid on each box, and the badge was placed on it to completely cover the contents.

The box after the said operation was laminated | stacked on the inside of the normal simple mushroom cultivator of the fungus area of 60 pyeong using a cart. The stacking method is to place three boxes of badges on two, three, and four stages, except for the bottom of the mushroom cultivator, which is generally made up of four stages, and stacked them at a distance of about 15cm from each other. After the lamination was completed, all doors in the planter were sealed. After 14 hours, the first ventilation was performed. After that, the ventilation was performed every 2 hours for 2 days and the temperature of the medium was observed. The temperature of the medium started to fall from 3 days after lamination | stacking peaking at 67 degreeC. Ventilation was performed every 4 hours for 3 days from when the temperature of the medium started to fall. Three days after the temperature began to drop, the medium temperature dropped to 45 ° C. After confirming that the center temperature of the medium contained in the box dropped to an average of 45 ° C, immediately by operating a refrigerator installed in the grower, the temperature of the medium was quenched to 28 ° C.

A total of 1,300 boxes of medium are divided into 8 stages of the above average number of growers, and 160 bottles of 160 mushroom seeds (160 pounds, 705 varieties) are inoculated according to the detailed description above, and immediately covered with newspaper and groundwater so that the newspaper is sufficiently wet. Watering was also applied to the planter floor to provide sufficient room humidity, while incubation of the inoculum was started with the room temperature at 22 ° C.

11 days after the end of the inoculation work, the fungus mycelia identified by the naked eye was very good and the forces were strong. The thickness of the cover was 5cm, and after covering water of 300L, covering the vinyl and adjusting the room temperature of the grower to 20 ° C.

After 5 days of covering, all the fungus vinyl covered was peeled off and futo was applied. At the time of the third futo, the fungus mycelium appeared more than 40% on the cover, and the force was very good. After 7 hours of the last futo, 400L of 15 ° C of groundwater was irrigated onto the cover, and sufficient ventilation was performed to completely change the air of the entire cultivator. The room temperature was lowered to 16 ° C. Since then, two additional 300 liters of groundwater have been irrigated and ventilated simultaneously. In order to preserve the room temperature, a large blower was started to complete the ventilation as soon as possible and the ventilation was completed within 10 minutes.

Within 12 days after the first irrigation, mushrooms appeared on the fungus. The first cycle yielded 1,140 kilograms of 570 boxes on a special basis in 2kg paper boxes, the government recommended packaging. This is indeed a surprising quantity and quality compared to conventional rice straw cultivation or tailings cotton wool medium. Of course, the difference in yields between varieties is so large that more crops can be harvested if other varieties are planted, but the white mushrooms of the bisporus strain, which are imported varieties, may have a large yield. This is not only suitable for Western tastes, but also developed for mechanical cultivation, which shows a significant decline in quality. In light of the reality of the cultivation of the cultivated farmers, which are unable to meet the demands of their efforts, the success of the cultivation of the Agaricus bitorquis 705 cultivar, which is perfect for our taste and traded at twice the price of imported varieties. Suggests a new alternative to the country's mushroom cultivation industry It can be said.

If we look at the yield from conventional cultivation method, we can see that the total yield of 1 cycle is about 1,000kg based on the intermediate product, but this is only a statistical value that was established long before the acidification of rice straw was raised. A growing number of farmers who are less than 500kg are in the trend, and the white-based foreign imported breed (Agaricus bisporus), which grows relatively healthy even under poor media conditions, is flooding the domestic market.

In addition, in the case of the Republic of Korea Patent Publication No. 2003-59775, only the white varieties can be cultivated (these cases are failing in most farmers, and the originality of the affected farmers is accumulating.) 705 You cannot grow varieties. Thereafter, the yield for each cycle produced in the medium of the present invention is briefly described below.

2nd cycle: 400 boxes (based on goods) = 800kg

3rd cycle: 220 boxes (based on goods) = 440kg

4th cycle: 170 boxes (medium basis) = 340kg

5th cycle: 150 boxes (medium basis) = 300kg

6th cycle: 135 boxes (medium basis) = 270kg

7th cycle: 127 boxes (medium basis) = 254kg

Eighth Cycle: 97 Boxes (Special Standards) = 194kg

9th cycle: 45 boxes (based on yawning) = 90 kg

  Total 1,914 total cycles = 3,828 kg

Harvesting continued after the ninth cycle, but was abolished because of economic viability against current market prices. In reality, there are individual differences in consideration of the expenses and labor cost of cultivating farming farmers, but generally, until the 7th cycle, it is considered economical.

Example 2 Cultivation of Mushroom Mushroom according to the Invention

In general, the medium was prepared under the same conditions as in Example 1, but the process was carried out differently after the first step of the second process. Therefore, the second embodiment will be described from the ninth step of the first embodiment.

In Example 1, the gypsum mixed in a stirrer was put in a box, which was shaken off with a cotton wool, but in this case, the medium was not placed in a box but immediately loaded onto a bed using a cart or the like.

First, the medium was laid on the top of the top of the top of the top of the top of the top of the top of the top of the top of the top of the bed, which was 180 cm long and 2,000 cm wide with 24,000 perforations with a diameter of 3 mm. In addition, considering the deviation between the top and bottom of the room temperature, in this case, the inventors suggested that the remaining 2, 3, and 4 stages, except for the lowest bottom of the general mushroom mushroom cultivator, were produced in a total of 4 stages with 4000 kg of dry weight as the inventors' proposal. After dividing and preparing the prepared medium, and again covered with a pile of medium loaded with hydride vinyl of 180cm in length 180cm in length 24,000 perforations of the diameter of 3mm was opened. Ventilation was carried out in the same manner as in step 11 in Example 1. After 8 hours of the first ventilation, the median temperature of the medium deposited on the fungus reached 68 ° C. In order to control the continuous increase in temperature, the freezer in the grower was operated until the room temperature reached 50 ° C or lower.

Thereafter, ventilation was repeated every four hours, and after three days, the medium temperature began to fall. After that, it was interrupted and oxygen was supplied to the medium in which the growth of various aerobic microorganisms was continued by injecting fresh air into a closed grower using a small blower. 48 hours after the ventilation was stopped, the center temperature of the medium was lowered to 43 ℃, so all the doors of the growers were opened and the temperature was rapidly lowered to lower the center temperature of the medium to 26 ℃, and then peeled off the vinyl covering the surface of the medium. Inoculation work of the mushroom spawn was carried out in the same manner as in the case of Example 1.

At this time, the mushroom seed spawn inoculation method was mixed inoculation, the spawn seed was used 250 bottles (705 varieties, 250 pounds).

The cultivation and cover of the mushroom spawn, and the method of timing and irrigation, and the irrigation amount and the timing of the ventilation is the same as in the case of Example 1 below, but the quality and quantity of the mushroom produced less than a few.

First cycle; 482 boxes (Special standard) = 964kg

Second cycle; 346 boxes (based on product) = 692kg

Third cycle; 215 boxes (based on goods) = 430 kg

Fourth cycle; 190 cartons (medium basis) = 380kg

Fifth cycle; 116 boxes (medium size) = 232kg

Fifth cycle; 90 boxes (medium size) = 180kg

Sixth cycle; 63 Boxes (Yawn) = 126kg

Seventh cycle; 38 boxes (based on yawn) = 76kg

Eighth cycle; 27 boxes (based on yawn) = 54kg

  Total 1,567 boxes 3,134 kg

As can be seen from the above, it can be seen that the case of Example 2 is inferior in quality quantity compared to Example 1, but this may be slightly different due to differences in cultivation environment or changes in weather conditions. Compared to the cultivation method using another medium, Example 2 may also be a very big success.

As described above, the present invention has the effect of reducing the total cost as well as the labor costs by reducing the time required for the entire process of producing the medium for cultivating mushrooms to less than about 50% compared to conventional production of medium. By eliminating the process of sterilization in conventional cultivation and producing mushroom mushroom medium using self-heating of thermophilic microorganisms, it can not only reduce the cost of expensive steam boilers and sterilizers but also use no oil such as heating oil. In this way, the manufacturing cost can be drastically reduced, thereby improving the international competitiveness in the mushroom mushroom cultivation industry, and the organic medium activating the ecological ring of microorganisms can be produced in the whole process of the culture of the mushroom mushroom cultivation industry. Use less than 50% of the spawn used in cultivation But will the result be able to contribute to a very useful and keuna great development in the country mushroom cultivation industry opened the way for the high-yielding mushroom in a much more superior quality.

This completely eliminates the enormous cost of 60 ° C pasteurization in the Korean cultivation industry, which has been regarded as gold and jade in the past 30 years of history, and understands the production of culture for cultivating mushrooms as an organic category. By inventing, we proposed an alternative to actively deal with Chinese mushrooms, which are being pushed in the aftermath of the opening of agricultural imports. It will be said that the way to provide high-quality mushrooms for the tastes of consumers who prefer to eat at a relatively low price.

Claims (2)

Containing 70 to 80% water in 4,000 parts by weight of the cotton; Performing a regression to discharge excess moisture contained in the direction of gravity; After one day, the step of depositing 100 parts by weight of hydrated lime or quicklime in a mixture of 240 to 500 parts by weight of rice bran and 500 to 1000 parts by weight of scale; 1 day after the main deposition, adding 100 parts by weight of urea fertilizer and 50 parts by weight of the compound fertilizer to perform the first flipping; A second inversion by spraying evenly 400 to 800 parts by weight of germanium dissolved water and 5 to 15 parts by weight of elvan powder after one day from the first inversion; Supplying 400 to 800 parts by weight of seawater or brine within one day from the second inversion and the third inversion; After the third flip, shaking the medium after the third flip in the air twice a day so that the core temperature of the stack of media does not exceed 60 ° C .; A day after the fourth flipping, evenly spraying anhydrous gypsum 200-250 parts by weight to the fifth flipping; Shaking the medium deposited outdoors to completely discharge residual ammonia gas and carbon dioxide gas and supplying oxygen to cool the temperature of the medium to about 30 ° C .; The cooled medium is laid with 120cm wide and 80cm long heiden vinyl with 1000 ~ 1200 holes perforated with a diameter of 3mm ~ 5mm inside a plastic box, and 15-15 parts of medium is filled, and the contents inside the box are completely covered. Granulation step; The boxes are moved inside the fermentation chamber so that the space between the boxes is 15 ~ 20cm apart from each other.The lowest box is 1.5cm or more from the ground and lamination is performed up to 9 steps, but 3 steps, 4 steps, 6 steps, and 7 steps. Laminating at intervals of 30 cm from each other; Performing a primary ventilation when the temperature of the medium reaches 50 to 60 ° C. after 12 to 18 hours after lamination; Closing all doors of the fermentation chamber and waiting 10-12 hours; Performing a secondary ventilation after opening the door of the fermentation chamber after the time elapses; Ventilating so that the air in the room may change every 3 hours after the secondary ventilation, and stopping the ventilation when the temperature of the medium naturally reaches 45 ° C; Method of producing a mushroom mushroom cultivation medium, characterized in that it comprises a step of rapidly lowering the temperature of the medium to 20 ~ 25 ℃ using a cooler after the ventilation stops. A method of cultivating mushroom mushrooms, which is inoculated using 200 liters (200 pounds) or less of mushroom seed spawn on the basis of a simple cultivation area on the medium of post-fermentation finished in claim 1, which is grown.