KR20050026297A - Skin anti-aging cosmetics composit containing oriental herb extract and extraction method of the same - Google Patents

Abstract

A skin anti-aging cosmetic composition comprising oriental medicinal herb extracts and an preparation method thereof are provided, which oriental medicinal herb extracts have improved activity for aging inhibition, cell proliferation, elastase inhibition, antioxidation and tyrosinase inhibition, so that the composition comprising the oriental medicinal herb extracts has improved wrinkle enhancement and elasticity improving effects. The skin anti-aging cosmetic composition comprises the oriental medicinal herb extracts of Prunus persica(L) BATSCH., Paeonia suffruticosa Andrews., Cyperus rotundus Linne and Magnolia obovata, wherein the composition has a formulation selected from skin, lotion, essence, cream, pack, foundation and make up base. The method for preparing the skin anti-aging cosmetic composition comprising the oriental medicinal herb extracts comprises the steps of: (a) preparing a mixture of Prunus persica(L) BATSCH., Paeonia suffruticosa Andrews., Cyperus rotundus Linne and Magnolia obovata; and (b) adding an extraction solvent into the mixture of the oriental medicinal herbs and heating them at 5 to 37 deg. C for 1 to 15 days, wherein the solvent is one or more selected from purified water, C1-C4 anhydrous or hydrous methanol, ethanol, propyleneglycol, propylalcohol, butyleneglycol, butylalcohol and glycerin.

Description

Skin anti-aging cosmetics composit containing oriental herb extract and extraction method of the same}

The present invention relates to a cosmetic composition containing the herbal medicine extract and a method for extracting the same, and more specifically, as a herbal medicine mainly used in herbal medicine, to control the Gi and It is a diuretic medicine that helps to smooth the flow of blood by regulating yigi medicine and blood, and to replenish blood, and to stop bleeding. The present invention relates to a cosmetic composition containing an herbal extract using an active ingredient having an anti-aging effect from herbal mixtures of vinegar, bark skin, saengbuja, and huhu, classified into iv), and a method of extracting the same.

The human skin is an indispensable organ in our lives and one of the most susceptible areas. This is because skin is always exposed to oxygen, sunlight, bacteria and pollutants, and since sebum or photosensitive substances are present on the skin, photobiological reactions involving free radicals and lipid peroxides are prone to damage. The skin is also the site of direct physical and chemical injury from a variety of external factors. Therefore, among the human body, the skin is always exposed to an aging environment, and in addition to the desire for life extension, methods for delaying skin aging have been devised.

Skin aging is manifested in various phenomena such as dryness, spots, blotch, and wrinkles, and can be accelerated by external environmental effects such as ultraviolet rays, low temperature and low humidity conditions. In general, the drying phenomenon is remarkable as the age increases, starting from the thirties, and the decrease in the water defense and retention capacity of the stratum corneum, or the decrease in the amount of epidermal lipids, is mainly due to atrophic changes due to epidermal or dermal aging. It becomes easy to produce dry skin easily. The blemishes and black mushrooms start from about 30 to 40 years of age and increase with increasing age (face, back, etc.). The main reason for this is a decrease in the transparency of the skin. And accumulation of skin, ② decrease in water retention capacity of the stratum corneum, or decrease in skin clarity due to the decrease of epidermal lipids, and ③ functional changes in the capillary system. Wrinkles also occur around the age of 25 years, and the skin is relaxed, and the secondary bone deepening of the bones is formed between them. The main causes are changes in dermal collagen fibers, elastic fibers and substrates, reduction of subcutaneous fat, exposure to ultraviolet rays, etc. Can be mentioned.

Due to skin damage caused by such external environment or internal mechanisms, efforts to suppress the appearance of skin aging or wrinkles have emerged as a huge concern in the cosmetic manufacturing industry. Various efforts have been made to include a predetermined extract using the herbal medicine as an ingredient of the cosmetics, and in particular, the present inventors, in order to achieve the above-described present invention, first of all, medicinal medicinal herbs, bleeding drugs to control blood Among 19 kinds of medicinal products, 19 kinds of extracts including Salmon, Mugwort, Hyangbuja, and Guryeok, Salvia, Angelica, Sandalwood, Baekchul, Sancho, Siho, Turmeric, Motherwort, Ginseng, Peony, Sculptor, Cheongung, Cheongpi, Safflower, and Astragalus After extraction with a solvent, the cell proliferation effect, elastase inhibitory effect, antioxidant Effect and a tee when while having a technical background comes browse through the tyrosinase inhibitory activity, will the present invention is made in view.

In the present invention, inhibiting the aging phenomenon of the skin that can be caused by the above-mentioned factors in the human skin, and enhance the cell proliferation effect, elastase inhibitory effect, antioxidant effect and whitening effect without causing other side effects There is a technical problem to be achieved by the present invention for applying to the skin cosmetic after selectively extracting the herbal medicines to make. In order to achieve this technical problem, the herbal medicine mainly used in oriental medicine is divided into two kinds of medicines, which are divided into two kinds of medicines, one that controls qi and one that controls blood. As a result of the study of herbal medicines having an anti-aging effect, it is an object of the present invention to provide a cosmetic composition containing the herbal medicine extract and a method for extracting the same.

Cosmetic composition containing the herbal medicine extract provided to achieve the above object of the present invention is characterized by using the active ingredient extracted from the herbal medicine mixture of cabbage, bark skin, hyangbuja and hakbak.

At this time, the content of each of the phosphorus, bark skin, saengbuja and thick gourd is preferably 10 to 50% by weight relative to the total weight of the herbal mixture before extraction. When the content of any one herbal medicine in the herbal mixture before extraction is less than 10% by weight, the content of the herbal medicine is relatively low, it is not preferable because the anti-aging effect of the original desired skin is not sufficiently achieved, the herbal medicine mixture before extraction If the content of any one of the herbal medicines in more than 50% by weight is relatively undesirably low content of other herbal medicines can not sufficiently achieve the anti-aging effect of the original desired skin.

On the other hand, in the case of applying the active ingredient extracted from the herbal medicine mixture to the cosmetic composition based on the total weight of the cosmetic composition may exhibit an effect sufficient to prevent skin aging. If the content of the extracted cosmetic active ingredient is less than 0.001% by weight relative to the total weight of the cosmetic, it is not preferable to achieve a sufficient anti-aging effect of the original desired skin, the content of the extracted cosmetic active ingredient is the total cosmetic weight If it exceeds 10% by weight, it may cause a problem in the stability of the cosmetics, which is not preferable due to the disadvantage of not exhibiting the cosmetic effect or sufficient cosmetic effect when using the cosmetics.

The cosmetic composition prepared by containing the herbal extract may be prepared in various formulations such as skins, lotions, essences, creams, packs, foundations, and makeup bases, and specifically, liquid, cream, paste, and It can be applied in various properties such as a solid phase, and can be applied to a conventional cosmetic preparation method.

It is apparent that the cosmetic composition and cosmetics prepared by extracting an active ingredient for preventing skin aging from the herbal mixture may be used in various forms for preventing skin aging, such as skins, lotions, essences, creams, and packs. It can be prepared in various product formulations such as foundations and makeup bases. In terms of physical properties, it can be applied in various properties such as liquid, cream, paste and solid, and it is also obvious that it can be applied to a conventional cosmetic preparation method.

Extraction method of the cosmetic composition containing the herbal medicine extract provided to achieve the above object of the present invention, (a) is a step of preparing a herbal mixture consisting of bark skin, hyangbuja and hakbak; And (b) adding a predetermined extraction solvent to the prepared herbal mixture and heating to extract the active ingredient.

At this time, in the step (a), the content of each of the phosphorus, the bark skin, saengbuja and bakbak is preferably mixed in a ratio of 10 to 50% by weight relative to the total weight of the herbal mixture before extraction. When the content of any one herbal medicine in the herbal mixture before extraction is less than 10% by weight, the content of the herbal medicine is relatively low, it is not preferable because the anti-aging effect of the original desired skin is not sufficiently achieved, the herbal medicine mixture before extraction If the content of any one of the herbal medicines in more than 50% by weight is relatively undesirably low content of other herbal medicines can not sufficiently achieve the anti-aging effect of the original desired skin.

In the step (b), the extraction solvent is one selected from a solvent group including purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propylene glycol, propyl alcohol, butylene glycol, butyl alcohol, and glycerin. The pure solvent of or two or more selected arbitrarily selected among them is a mixed solvent, the selected extraction solvent is preferably added in an amount of 1 to 20 times the total weight of the herbal mixture. If the content of the selected extraction solvent is less than 1 times the total weight of the herbal medicine mixture, the whole herbal medicine is difficult to be evenly extracted, and thus, it is not preferable to achieve the desired extraction effect, and the content of the selected extraction solvent is 20% of the total weight of the herbal medicine mixture. If it exceeds the pear content of the active ingredient after extraction may be low is not desirable to achieve the original desired extraction effect.

On the other hand, step (b) after the extraction solvent is added to the herbal medicine mixture, proceeding by the method of immersion extraction for 1 to 15 days at a temperature condition of 5 to 37 ℃, or after adding the extraction solvent to the herbal medicine mixture, the cooling capacitor It is preferable to proceed with a method of extracting by adding heat for 1 to 20 hours at a temperature of 50 to 95 ℃ after mounting. In the case of extraction by adding the extraction solvent to the herbal mixture, both room temperature extraction or heat extraction are preferred, but whether the extraction temperature is too low or the extraction time is too short, whether the extraction method is too low or the extraction time is too effective, the effective ingredient is not effectively extracted. It is not preferable because the extraction effect can not be achieved, and even if the extraction temperature is higher or the extraction time is longer than the condition that the effective ingredient is extracted effectively, the extracted ingredient does not affect the effective condition but the effective ingredient is extracted effectively. Extraction under more severe conditions is undesirable.

The herbal extracts contained in the skin anti-aging cosmetics of the present invention can be easily purchased on the market for use in manufacturing, and the well-known literature on the ingredients and the use of herbs (Ilwolseok, 1991), Korean Herbal Paintings (Kyohaksa, 1998), Medicinal Herbal Botany (Hakmun Publishing Co., 1999) and Medicinal Herbal Standards Collection (Korea Pharmaceutical Import & Export Association & Korea Research Institute for Drug Testing, 2000) are as follows.

Doin is a seed of the Rosaceae larch, Prunus persica (L.) B., which contains emulsion, amygdalin, and linolenic acid. Doin is used in Chinese medicine for menstrual pain, amenorrhea, postpartum abdominal pain when it is in the body, and it is used for pain, anti-inflammatory, detoxification, mitigation, bruises, boils, internal bleeding, etc. It is known to have an effect of stopping coughing, and to remove peach for the purpose of eliminating phlegm, it is known to be effective for constipation and easy bowel movements due to its rich fat. Cain is known to improve the color of the skin, whitening and anti-inflammatory action, and adds shine to skin.

Root bark is the root of Paeoniaceae perennial peony Paeonia suffruticosa A. Paeonol and its glycosides paeonoside, paeonolide, and peon It contains paeoniflorin. This is an essential drug of gynecological drugs, sedative action, fever lowering action, pain relief action, anti-inflammatory action and bloody action and blood pressure lowering action has been reported. Bark skin is known to show anti-inflammatory effect and blood circulation improvement when used in combination with causality. On the other hand, the efficacy of the endothelial skin associated with cosmetics has a boil treatment effect by anti-inflammatory action, tyrosinase activity inhibitory effect, it is known to prevent skin aging by promoting blood circulation and anti-inflammatory action and astringent action.

Hyangbuja is the root stem of Cyperaceae perennial herb, Cyperus rotundus L., essential oil, alkaloid, flavonoid, sugar, pectin, etc. It contains. Flow extract of the root stem of Hyangbu is known to improve pain, pain relief, uterine tension, cardiac action, urination and blood circulation. In oriental medicine, Hyangbuja is a pain medication and a blood shading drug, which is widely used for dysmenorrhea and postpartum gynecological diseases. It is also used in antipyretic medications and digestive medicines for colds, head aches and embryos. Related to cosmetics, Hyangbuja shows strong antibacterial effects on skin fungi, smoothes blood circulation, brightens the color, and is known to prevent skin aging due to antioxidant and anti-inflammatory effects.

Thick gourd is the bark of Magnoliaceae leaves of Magnolia obovata T. It contains magnolol, honokiol and machiol. Herbal ginseng in the oriental medicine has a row effect (only the abdominal intestine, it is effective in indigestion, it is used when the stomach is bloated and food is reluctant. And the effects of calming asthma are known. With regard to cosmetics, hoobak is known for its antimicrobial and antioxidant effects that inhibit skin aging.

Hereinafter, specific examples such as examples and experimental examples will be described in order to specifically describe the present invention. However, embodiments or experimental examples according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the examples described below. The examples and experimental examples according to the present invention are provided only in order to more completely explain the present invention to those skilled in the art, and various equivalents which may be substituted for them at the time of the present application and It should be understood that there may be variations.

Extraction Examples 1-5

250 g of selected grains, 250 g of bark skin, 250 g of hyangbuja and 250 g of thick gourd each were put into an extractor, 5 kg of each of the extraction solvents as shown in Table 1 was added thereto, and extracted by immersion at room temperature for 6 days, followed by filtration with 400 mesh filter cloth. The mixture was left at 4 ° C. for 7 days for low temperature aging, and then filtered through a 0.45 μm filter. The extract filtrate was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor to obtain 33.2 g of herbal extracts as shown in Table 1 below.

Extraction Example Extraction solvent Herbal Medicine Extract Amount (g) One 30% water ethanol 33.2 2 50% water ethanol 34.5 3 70% water ethanol 35.4 4 90% water ethanol 37.4 5 30% water butylene glycol 22.8

Experimental Example 1 <MTT experiment using fibroblasts>

MTT test method using fibroblasts [3- (4,5) to find out the effects on the cell proliferation effect and toxicity to assay the anti-aging effect of the medicinal herb extracts obtained according to the respective extraction examples of the present invention -dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide reduction method] was performed.

First, human normal fibroblasts are dispensed into 1 × 10 ⁴ cells in each well of a 96-well plate and cultured first in DMEM (Sigma) medium for 24 hours. do. After the primary culture step, the concentration of the herbal extracts obtained in the extraction example 1 was adjusted as shown in Table 1, and then cultured in a fetal bovine serum-free medium for 2 hours. After the second incubation step, 100 μl of MTT solution (1 mg / ml, Sigma) was added to the culture medium, and left for 4 hours, and then the medium was removed. 100 μl of a dimethyl sulfoxide (Sigma) solution is added to each well, and then the absorbance is measured at 490 nm in a microplate reader. The control group in the MTT experiment was set to the culture medium without the herbal extracts.

Table 2 below shows the experimental results of using the herbal extracts obtained in the extraction example 1 by varying the concentration, and calculating the viability of the cells using numerical formula 1 below.

Concentration (µg / ml) Cell proliferation (%) 10 104.2 to 105.7 100 108.4 to 111.8 250 127.7 to 130.6 500 135.3 to 140.1

As shown in Table 2, the fibroblasts treated with the medicinal herb extract according to the extracting example 1 have a cell proliferation effect even at a small concentration of 10 µg / ml, and particularly, a cell proliferation effect is promoted even at a concentration of 500 µg / ml. Able to know. From these results, it can be confirmed that the herbal extract according to the embodiment of the present invention has no toxicity and has a cell proliferation effect.

On the other hand, according to the MTT test method, in particular, when the concentration of the herbal extract according to the extraction example 1 was 500㎍ / ㎖ was found that the cell proliferation effect is further promoted, the MTT experiment and Proceed in the same manner, the 500 ㎍ / ㎖ extract of each of the herbal extracts 1 to 5 obtained from the extraction examples 1 to 5, respectively, treated with the fibroblast culture medium, and then the viability of the fibroblasts according to the formula (1) The measurement results are shown in Table 3 below.

Medicinal Herb Extracts Cell proliferation (%) One 135.3 to 140.1 2 134.2 to 135.7 3 128.4 to 131.8 4 127.7 to 130.6 5 133.3 to 138.1

As shown in the results shown in Table 3, the cell proliferation of the herbal extracts (parts 1 to 5) extracted by the various embodiments according to the present invention is not treated with the extract (example not treated with herbal extracts) Compared with the improvement effect of about 28 to 38% relative, it was confirmed that there is no cytotoxicity in detail.

As a comparison value for the cell proliferation ability and cell nontoxicity according to the present invention, the cell proliferation ability of the yulpi extract (250㎍ / ㎖) obtained under the same solvent condition as in Example 3 but similar extraction conditions was 128.7%, the Gaza extract (250㎍ / ㎖) The cell proliferation of 118.2% (Korean Patent Publication No. 2002-0001913), and the extraction solvent is the same as Example 3, but the extraction conditions are slightly different, medicinal herb mixed extract consisting of jade, lotus, peony, lily, and sulfur In view of the known data presented in 124.2 to 130.3% (Korean Patent Publication No. 10-0193105), it will be apparent that the cell proliferation ability can be objectively confirmed by the excellent cell proliferation effect of the herbal extract according to the present invention.

Experimental Example 2-<Ellastays Low Resolution Experiment>

The herbal extract obtained in accordance with the extraction example of the present invention was subjected to the Elastase Inhibition Activity Test (Elastase Inhibition Activity Test) as a test for inhibiting and improving skin wrinkles.

A method for measuring the activity of elastine, an enzyme that degrades elastin, is used to determine the color change caused by the degradation of p-nitroaniline using N-Succinyl-Ala-Ala-Ala p-nitroaniline It is a method of measuring elastase activity by measuring absorbance at a wavelength of 410 nm. PH 8.0, 0.267M Trizma-HCl (Sigma), substrate solution 8.8 mM N-Succinyl- (Ala) ₃ p-nitroaniline (Sigma), enzyme solution 10 μg / ml (Sigma) Used as a concentration. 60 μl of buffer solution, 20 μl of substrate solution and each of the herbal extracts obtained from the respective extraction examples 1 to 5 (part numbers 1 to 5) were dissolved in purified water for each concentration, and then mixed with 100 μl of sample solution, followed by 20 μl of enzyme solution. The reaction was carried out in a 25 ° C. constant temperature water bath for 15 minutes and the amount of p-nitroaniline was measured at a wavelength of 405 nm using a microplate reader.

On the other hand, the control for measuring the elastase activity was measured in the same way to put the purified water instead of the herbal extracts, and set the case of obtaining a color correction value for each of the purified water instead of the enzyme solution.

Elastase inhibition rate was calculated numerically by using the following Equation 2 shown in Table 4 below. In Table 4 below, IC ₅₀ is the concentration of the extract sample required to achieve 50% of the elastase inhibition rate, which is a value used in comparison with other components, and the smaller the value, the higher the inhibition rate.

Medicinal Herb Extracts Elastase inhibitory activity, IC ₅₀ (mg) One 0.032 2 0.040 3 0.037 4 0.039 5 0.053

As shown in the results shown in Table 4, it was found that the elastase inhibition rate of the herbal extracts (parts 1 to 5) extracted by various examples according to the present invention was 0.031 to 0.053 mg (IC ₅₀ ). Can be.

On the other hand, the elastase inhibition rate of the extract (Sections 1 to 5) obtained by extracting each of Doin, Bark skin, Hyangbuja, and Hulk as a single herbal medicine under the same conditions as the extraction Example 1, 0.052 mg, Compared with 0.126mg, ginseng extract 0.025mg, hyangbuja extract 0.105mg, the mixed herbal medicine extract as in the present invention has the effect of increasing the elastase inhibitory effect compared to using the herbal medicine extract alone You can see that.

Experimental Example 3- <free radical scavenging test>

The herbal extracts obtained according to the embodiment of the present invention were subjected to a free radical scavenging activity test in order to determine the antioxidant effect in the assay for preventing skin aging effects. Free radical scavenging test is a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299 to 303 (1993)), a stable free radical DPPH (1,1-Diphenyl-2-picrylhydrazyl radical (Sigma) was used.

First, each of the herbal extracts (parts 1 to 5) obtained according to the extraction examples 1 to 5 in 1 ml of 0.2 mM DPPH solution was dissolved in methanol, mixed to 2 ml, and left to stand at room temperature for 10 minutes. The absorbance is then measured at 525 nm.

On the other hand, the control in the free radical scavenging test was measured by putting methanol instead of the sample solution, and the methanol was put in place of the DPPH solution was set to obtain the respective color correction value for the herbal extract sample and the control.

The free radical scavenging ratio was calculated numerically using Equation 3 below and is shown in Table 5 below. In Table 5, SC ₅₀ is a concentration of a sample required to achieve 50% free radical scavenging ratio, which is a value used in comparison with other component substances, and the smaller the value, the higher the scavenging ratio.

Medicinal Herb Extracts Layuradical Scavenging Rate, SC ₅₀ (mg) One 0.041 2 0.039 3 0.038 4 0.033 5 0.049

As in the results shown in Table 5, the herbal extract extracted by the various extraction examples according to the present invention (Sections 1 to 5), the ratio of the ratio of each of the sample is 0.033 to 0.049 mg (SC ₅₀ ) excellent effect It can be confirmed that the.

Meanwhile, as a comparison value, the free radical scavenging ratio of the extract of 'fat milk' obtained under the same extraction conditions as the extraction example 1 but under similar extraction conditions was 0.024 mg (SC ₅₀ ), and ascorbic acid was 0.027 mg ( SC ₅₀ ) (Korean Patent Application Laid-Open No. 2002-0047762), and the extract of 'Hwanhwanpi' extracted under the same conditions as fat or milk has been disclosed as 0.099 mg (SC ₅₀ ) (Korean Patent Publication No. 2002-0080657). Therefore, it can be seen that the antioxidant effect of the herbal extract extracted by various embodiments according to the present invention corresponds to ascorbic acid, which is a powerful synthetic oxidant.

Experimental Example 4- <Tyrosinase Inhibition Test>

The Tyrosinase Activity Inhibition Test was performed on the herbal extracts obtained according to the extraction examples of the present invention to determine the whitening effect in the assay for preventing skin aging effects.

Tyrosinase is an enzyme that promotes the oxidation process of tyrosine in vivo and helps melanin pigments to be produced. Therefore, the effect of the sample is to inhibit the function of the enzymes, thereby preventing the production of melanin. It is evaluated to the extent to suppress. Tyrosinase (Fluka) was used in the mushroom was isolated and purified, L-tyrosine (Fluka) substrate was dissolved in 0.1M sodium phosphate buffer solution was used to make 0.3 mg / ㎖ concentration. 0.5ml of each sample of each herbal extract extracted according to the extraction examples 1 to 5 in the present invention (parts 1 to 5) adjusted to an appropriate concentration, tyrosine 1.0ml of the substrate, 1.1ml of the buffer well Mix and leave for 10 minutes at 37 ℃. 0.1 ml of 1,600 U / ml tyrosinase was added thereto and reacted at 37 ° C. for 10 minutes, and the reaction was stopped by cooling in ice water for 2 to 3 minutes to measure the absorbance at 480 nm.

The control group for the tyrosinase inhibition test was measured in the same manner by putting the buffer solution instead of the sample solution, and was set to the case where each color correction value for the herbal extract sample and the control group was added to the buffer solution instead of tyrosinase. To calculate the tyrosinase inhibition rate by using the following Equation 4 shown in Table 6 below. In Table 6 below, IC ₅₀ is a concentration of the extract sample required to achieve 50% tyrosinase inhibition rate, which is a value used in comparison with other components, and the smaller the value, the higher the inhibition rate.

Lettuceum known as a whitening agent was tested under the same conditions for the extract extracted under the same conditions as the extraction example 1 described in Table 6 with the results.

Medicinal Herb Extracts Tyrosinase Inhibition Rate, IC ₅₀ (mg) One 0.117 2 0.103 3 0.092 4 0.095 5 0.146 Lettuce extract 0.232

As shown in the results of Table 6, the tyrosinase inhibition rate of each herbal extract (Section No. 1 to 5) extracted by various extraction examples according to the present invention is 0.092 to 0.146 mg (IC ₅₀ ), in general As the value of the baekbaekpi extract known as a whitening agent is lower than that, it was confirmed that the superior tyrosinase inhibitory effect.

Formulation Example 1 and Comparative Example 1

The composition of the flexible cosmetic lotion containing the herbal medicine extract according to the extraction example 1 was prepared as shown in Table 7 below. At this time, the unit of component content is weight%. On the other hand, among the components identified by each component number in Table 7 below, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 6 were added, and components 8 to 10 were dissolved in component 7. And component 11 were added in order to prepare a mixture.

Comparative Example 1 for Formulation Example 1, except for the component 11, except for the component composition or manufacturing method was set to proceed in the same manner.

number ingredient Formulation Example 1  Formulation Comparative Example 1 One Purified water Remaining amount Remaining amount 2 Carbomer 0.1 0.1 3 Hydroxyethyl Cellulose 0.1 0.1 4 Butylene Glycol 2.0 2.0 5 glycerin 1.0 1.0 6 Fiji-1500 0.5 0.5 7 ethanol 8.0 8.0 8 Polysorbate 80 0.2 0.2 9 Triethanolamine 0.1 0.1 10 antiseptic a very small amount a very small amount 11 Herbal extract 1 according to the extraction example 1 0.5 -

Formulation Example 2 and Comparative Example 2

The composition of the elastic lotion containing the herbal medicine extract according to the extraction example 1 was prepared as shown in Table 8. At this time, the unit of component content is weight%. On the other hand, among the components identified by each component number in Table 8 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 8 to 11 were dissolved and dispersed in component 12, and emulsified in those heated to 70 ° C. . Thereafter, the emulsified product was neutralized with component 13, cooled to a temperature of 56 ° C, and then component 14 was added thereto, stirred, and cooled to room temperature.

Comparative Example 2 with respect to Formulation Example 2, except for the herbal extract according to the extraction example 1 component 14, the composition and the manufacturing method of the rest proceeds to set the same.

number ingredient Formulation Example 1 Formulation Comparative Example 1 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate 0.5 0.5 3 Polysorbate 60 1.0 1.0 4 Sorbitan sequioleate 0.3 0.3 5 Liquid paraffin 6.0 6.0 6 Squalene 4.0 4.0 7 Macadamia Nut Oil 4.0 4.0 8 glycerin 5.0 5.0 9 Carbomer 0.1 0.1 10 Xanthan Gum 0.03 0.03 11 antiseptic a very small amount a very small amount 12 Purified water Remaining amount Remaining amount 13 Arginine 0.1 0.1 14 Herbal extract 1 according to the extraction example 1 1.0 -

Formulation Example 3 and Comparative Example 3

The composition of the elastic nutrition cream containing the herbal medicine extract according to the extraction example 1 was prepared as shown in Table 9. At this time, the unit of component content is weight%. On the other hand, among the components identified by the component numbers in Table 9 below, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 9 to 12 were dissolved and dispersed in component 13, and emulsified to those heated to 70 ° C. . Thereafter, the emulsified product was cooled to a temperature of 56 ° C., and then component 14 was added thereto, stirred, and cooled to room temperature.

Comparative Example 3 with respect to Formulation Example 3, except for the herbal medicine extract according to Extraction Example 1, which is component 14, the same as the ingredient composition or manufacturing method was set to proceed to the same.

number ingredient Formulation Example 3 Formulation Comparative Example 3 One Cetearyl Alcohol 1.5 1.5 2 Glyceryl Stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sequioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalene 8.0 8.0 7 Apricot Kernel Oil 4.0 4.0 8 Dimethicone 2.0 2.0 9 glycerin 5.0 5.0 10 Magnesium Aluminum Silicate 0.4 0.4 11 Xanthan Gum 0.03 0.03 12 antiseptic a very small amount a very small amount 13 Purified water Remaining amount Remaining amount 14 Herbal extract 1 according to the extraction example 1 2.0 -

<Wrinkle improvement effect and elasticity measurement experiment of skin>

Formulation Example 3 containing the herbal extract of Example 1 extract according to the present invention is applied to the actual skin and the comparative example is set as follows to the extent to affect the wrinkles and elasticity for the skin Relative comparative tests were performed as follows.

In other words, in order to measure the effect of increasing the elasticity and elasticity of the skin when the elastic nutrition cream of Formulation Example 3 and Comparative Example 3 is applied to the skin, the normal, oily, dry, complex skin of women over 20 years old To 20 owners, the elastic nutrition cream of Formulation Example 3 was applied to the left side of the face and the elastic nutrition cream of Comparative Formulation Example 3 was applied to the right side of the face for 12 weeks twice a day in the morning and evening. After continuous use, the skin wrinkle effect (Skin Visiometer SV 600, CK Electronic GmbH) and the elasticity measuring instrument (Cutometer SEM 575, CK Electronic GmbH) were used to measure wrinkle improvement and elasticity of the skin. And the results are shown in Table 10 below.

division Wrinkle improvement effect Elasticity Average depth of wrinkles (Rz) Roughness average (Ra) Skin adsorption degree (ds) Application example Before application 34.8 19.8 0.0543 After application 27.1 12.4 0.0368 Comparative Example Before application 34.2 19.3 0.0525 After application 33.8 18.5 0.0512

In Table 10, 'Rz' is Mean Depth of Roughness, and the sample made of silicon rubber is divided into 5 parts at the part to be measured for wrinkles. The mean value for the differences is shown. 'Ra' is the roughness average, which represents the average value of the wrinkle depth difference in the individual measurement sites (dividing the whole into 255 measurement point points) from the average value of the total wrinkle depths in the sample. As the values of 'Rz' and 'Ra' are lower, wrinkles are formed smaller, which means that the degree of skin aging is small.

From the results shown in Table 10, through the relative numerical calculations for the application examples before and after the application of the elastic nutrition cream, the wrinkle improvement effect of the 'Rz' value was improved by 22.12% (the application comparative example was improved by 1.17%). , 'Ra' value is 37.37% improvement of the wrinkle improvement effect (approximately 4.15% application of the comparative example), so the herbal medicine extract containing cosmetics according to the present invention has a significant effect on the skin wrinkle improvement effect It can be confirmed.

In addition, in Table 10, 'ds' represents the length of the skin (penetration depth of the skin into the aperture) into the cutometer measuring instrument, the numerical value indicating the degree of adsorption, the smaller the value is preferable. Elasticity effect by the 'ds' value is improved by 32.23% (applicability of comparative example 2.48%), it can be confirmed that the elasticity of the skin is significantly improved.

As a result, the fact that can be confirmed through the results shown in Table 10, the cosmetics containing the herbal extracts prepared by the extraction example according to the present invention to improve the skin wrinkle effect and elasticity effect, excellent effect of preventing skin aging It can be seen that.

Optimal embodiments of the present invention described above have been disclosed. Although specific terms have been used herein, they are used only for the purpose of describing the present invention and are not intended to limit the scope of the invention as defined in the claims or the claims.

Herbal medicine extract including cabbage, bark skin, hyangbuja and hoobak according to the present invention exhibits an excellent effect on the cell growth effect, anti-elastase inhibitory effect, antioxidant effect and tyrosinase inhibitory effect associated with anti-aging, cosmetics containing it The skin is effective in improving wrinkles and enhancing elasticity, which may lead to anti-aging effects of skin. Therefore, it is expected to be able to exert an excellent effect in the commercialization or commercialization of the product because it can exhibit a special functional effect, not just as a simplified cosmetic product.

Claims (10)

Cosmetic composition containing a herbal medicine extract, characterized in that using the active ingredient extracted from the herbal medicine mixture of cabbage, bark skin, hyangbuja and hubak. The cosmetic composition according to claim 1, wherein the content of each of the phosphorus, bark skin, saengbuja and hakbak is 10 to 50% by weight relative to the total weight of the herbal mixture before extraction. According to claim 1, wherein the content of the active ingredient cosmetic composition containing an herbal extract, characterized in that 0.001 to 10% by weight based on the total weight of the cosmetic composition. According to claim 1, wherein the cosmetic composition prepared by containing the herbal extract extract herbal medicines characterized in that it has any one selected from skins, lotions, essences, creams, packs, foundations and makeup bases Cosmetic composition containing a. The cosmetic composition according to any one of claims 1 to 4, wherein the cosmetic composition is used for preventing skin aging. (a) preparing a herbal mixture consisting of cabbage, bark skin, sauerkraut and thick pepper; And (b) adding a predetermined extraction solvent to the prepared herbal mixture and heating to extract the active ingredient; extracting a cosmetic composition containing a herbal extract, characterized in that proceeds. According to claim 6, wherein the contents of each of the indoin, neck skin, saengbuja and hubak in step (a) is a cosmetic composition containing a herbal extract, characterized in that the mixture of 10 to 50% by weight relative to the total weight of the herbal mixture before extraction Extraction method of the composition. The method of claim 6, wherein the extraction solvent in step (b) is purified water, a solvent containing anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propylene glycol, propyl alcohol, butylene glycol, butyl alcohol and glycerin One pure solvent selected from the group or a mixed solvent arbitrarily selected from two or more thereof is selected, and the selected extraction solvent contains the herbal medicine extract, characterized in that it is added in an amount of 1 to 20 times the total weight of the herbal medicine mixture. Extraction method of cosmetic composition. The method according to claim 6, wherein the step (b) comprises extracting the herbal medicines, characterized in that the extraction proceeds to the method of immersion extraction for 1 to 15 days at a temperature condition of 5 to 37 ℃ after adding the extraction solvent to the herbal medicine mixture Extraction method of cosmetic composition. The method according to claim 6, wherein the step (b) is performed by adding an extraction solvent to the herbal medicine mixture, mounting a cooling capacitor, and then applying heat for 1 to 20 hours at a temperature of 50 to 95 ° C. Extraction method of a cosmetic composition containing an herbal extract characterized in that.