KR20030024932A - Cosmetic composition containing centella asiatica extracts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Centella asiatica extract exhibiting excellent effects on cell proliferation and collagen and hyaluronic acid synthesis is provided which is excellent in prevention and improvement of skin aging. CONSTITUTION: The cosmetic composition contains 0.0001 to 10.0% by weight of an extract of Centella asiatica, based on the total weight of the cosmetic composition. The Centella asiatica extract is prepared by extracting 1 to 10 times with water, C1-4 anhydrous or hydrous lower alcohol or a mixed solvent of water and lower alcohol, acetone, ethyl acetate, chloroform and 1,3-butylene glycol, distilled under pressure, freeze-dried or spray-dried.

Description

Cosmetic composition containing Centella asiatica {Cosmetic composition containing centella asiatica extracts}

The present invention relates to a cosmetic composition containing centella extract.

In the field of cosmetics, the prevention of skin aging, that is, suppressing wrinkles or improving wrinkles is always a field of interest.

The main cause of this skin aging is the lack of estrogen. The main function of the female hormone estrogen on the skin is known to enhance skin activity and make the skin soft, elastic and supple. Specifically, estrogen stimulates fibroblasts in the dermis to promote collagen synthesis, is involved in collagen metabolism, inhibits over-glycation of collagen, and prevents post-translational modification. Induces the expression of collagenase, a collagenase, inhibits collagen degradation. In addition, by increasing the proliferative effect of the basal cells in the epidermis to make the epidermis thicker, it strengthens skin cell tissues, regulates sebaceous glands to inhibit the growth of sebaceous glands and reduces the production of sebum, especially hyaluronic acid, which is very important for skin moisture maintenance. Promote the synthesis of

When the estrogen deficiency having the above effects is depleted, a change in the skin occurs, and the typical phenomenon is skin drying. In the fibroblasts, the production of collagen, elastic fibers, etc. is suppressed, the skin becomes thinner, the elasticity of the skin is reduced, and the synthesis of hyaluronic acid is inhibited, thereby weakening the skin's water retention function. In addition, as collagen glycation increases, the crosslinking of collagen increases, which causes the skin to harden and decrease elasticity.

Unfortunately, the reproductive organs, the secretory sites for estrogen, stop producing estrogens before and after menopause, when the body's function gradually degenerates as it ages and the production function disappears. The production of estrogens is stopped and the balance of hormones is disrupted, which leads to uncompetitive effects of testosterone, a male hormone, which promotes endocrine aging due to estrogen deficiency. Endocrine aging is a type of skin aging that is physiological skin aging that occurs during menopause.

Recently, a number of studies have been reported on a solution for suppressing skin aging due to estrogen deficiency. A typical example is hormone replacement therapy that directly supplies estrogen. Direct supply of estrogens has been shown to have marked effects such as increased collagen synthesis, increased hyaluronic acid synthesis, and increased epidermal thickness due to epidermal cell proliferation.

However, other methods have been taken because cosmetics cannot directly use estrogen. Alternatives in cosmetics are not direct countermeasures due to estrogen deficiency, but indirect countermeasures using general inhibitors of skin aging such as moisturizers, aging inhibitors, free radical scavengers, and sunscreens.

However, these indirect measures have a limit in solving the fundamental problem of skin aging.

Therefore, in order to improve female skin during aging before and after menopause from a cosmetic perspective, it is important to develop an estrogen-like substance having collagen synthesis and hyaluronic acid synthesizing effect.

As a direct countermeasure against estrogen deficiency, the present inventors conducted research on native herbs to develop estrogen-like substances from plants, indicating that centella extracts showed excellent cell proliferation effect, collagen synthesis effect, and hyaluronic acid synthesis effect. Discovered and completed the present invention.

It is an object of the present invention to provide a cosmetic composition containing Centella extract excellent in preventing and improving skin aging.

The present invention relates to a cosmetic composition comprising the centella extract.

Specifically, the cosmetic composition of the present invention contains the Centella as an active ingredient extract of 0.0001 to 10.0% by weight, preferably 0.0005 to 10.0% by weight, and more preferably 0.005 to 5.0% by weight relative to the total dry weight of the cosmetic composition. Characterized in that. If the content is less than 0.0001% by weight, no obvious effect can be expected. If the content is more than 10.0% by weight, no apparent effect increases with the content.

Centella asiatica is a perennial plant belonging to the Apiaceae Scientific is sometimes called a 'Centella asiatica', jogaepul horseshoe pool. It is native to Madagascar Island in eastern Africa and has been reported to be effective in antipyretics, skin ulcers, trauma, tuberculosis and alopecia areata.

Centella asiatica extract of the present invention is a 1 to 10 times the weight of the dry weight in the centellae, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, or a mixed solvent of these lower alcohols and water, acetone, ethyl acetate, chloroform and Extracted with 1,3-butylene glycol, characterized in that the extract is prepared by distillation under reduced pressure and then lyophilization or spray drying.

The centella extract of the present invention may be added to basic cosmetics such as softening water, nutrient cream, massage cream, essence, pack, etc. according to a conventional method, and may be prepared as a cosmetic for preventing and improving skin aging.

The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

Example 1.

The centella was washed with purified water and dried. Then, 200 g of powdered centella pollen was added to 2.5 L of 70% ethanol solution, boiled for 4 hours in a reflux extractor equipped with a cooling capacitor, filtered through a 300 mesh filter cloth, and the residue was filtered. Extracted once more by the method. Each extract was combined, filtered through Whatman No. 5 filter paper, and the solvent was removed using a vacuum concentrator, and lyophilized to obtain a dry weight of 51.4 g.

Example 2.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was added to 2.5 L of 70% methanol, and the same method as in Example 1 was carried out to obtain a dry weight of 45.7 g.

Example 3.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was put in 2.5 L of purified water, and the same method as in Example 1 was carried out to obtain a dry weight of 41.8 g.

Example 4.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was placed in 2.5 L of 70% acetone, and the same method as in Example 1 was carried out to obtain a dry weight of 52.3 g.

Example 5.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was added to 2.5 L of normal butanol solution, and the same procedure as in Example 1 was carried out to obtain a dry weight of 38.3 g.

Example 6.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was added to 2.5 L of ethyl acetate solution, and the same method as in Example 1 was carried out to obtain a dry weight of 48.3 g.

Example 7.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was added to 2.5 L of 1,3-butylene glycol solution, and the same method as in Example 1 was carried out to obtain a dry weight of 31.9 g.

Example 8.

The centella was washed with purified water and dried, and then 200 g of powdered centella pollen was placed in 2.5 L of chloroform solution, and the same method as in Example 1 was carried out to obtain a dry weight of 43.1 g.

Prescription example 1 .

Prescription example of the flexible cosmetic water (skin) in the cosmetic composition containing the centellar extract of Example 1 of the present invention is as follows.

ingredient Content (% by weight) Centella extract 0.2 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 1.2 Polysorbate 20 3.0 ethanol 5.0 Propylparaben 0.1 Benzophenone-9 2.0 Carboxy Vinyl Polymer 1.0 Pf.962 0.2 Purified water Remaining amount system 100

Prescription example 2 .

Prescription example of the milk lotion in the cosmetic composition containing the centellar extract of Example 1 of the present invention is as follows.

ingredient Content (% by weight) Centella extract 0.2 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Propylparaben 0.1 Carboxy Vinyl Polymer 1.0 Pf.Kn-921 0.3 Purified water Remaining amount system 100

Prescription example 3 .

Prescription example of nutrition cream in the cosmetic composition including the centella extract of Example 4 of the present invention is as follows.

ingredient Content (% by weight) Centella extract 0.2 vaseline 7.0 glycerin 4.0 Triethanolamine 0.5 Tocopheryl Acetate 0.1 Liquid paraffin 10.0 Squalane 3.0 Beeswax 2.0 Polysorbate 60 2.0 Sorbitan sesquioleate 2.5 Propylparaben 0.05 Carboxy Vinyl Polymer 1.0 Pf.Kn-942 0.2 Purified water Remaining amount system 100

Prescription example 4 .

Prescription example of a massage cream in a cosmetic containing centella extract of Example 1 of the present invention is as follows.

ingredient Content (% by weight) Centella extract 0.3 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Tocopheryl Acetate 0.1 Liquid paraffin 30.0 Cetearyl Alcohol 2.0 Beeswax 2.0 Polysorbate 60 2.1 Sorbitan sesquioleate 2.5 Propylparaben 0.1 Carboxy Vinyl Polymer 1.0 Pf.951 0.2 Purified water Remaining amount system 100

Prescription Example 5.

Prescription example of the pack in the cosmetic containing the centellar extract of Example 4 of the present invention is as follows.

ingredient Content (% by weight) Centella extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 glycerin 4.5 Cabopol 1.0 Polyoxide 0.2 Pf.Kn-943 0.2 Purified water Remaining amount system 100

Experimental Example 1. Cell growth effect of Centella asiatica extract

(1) Experimental method

Human normal fibroblasts were inoculated to ¹ × 10 ⁴ cells in each well of a 96-well microplate and incubated in DMEM medium for 24 hours. After incubation, the centella extract prepared in Examples 1 to 8 was replaced with DM medium without serum adjusted to a final concentration of 250 μg / ml, followed by further incubation for 24 hours. In Table 1, the control group was cultured without adding centella extract. 10 [mu] l of MTT solution [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide: 5 mg / ml] was added and left for 4 hours, and then the medium portion was discarded. 100 μl of dimethyl sulfoxide solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 500 nm with a microplate reader. The same procedure was repeated three times.

(2) Experiment result

The cell proliferation effect was calculated by the following formula and the results are shown in Table 1 below.

extract Absorbance (500nm) Cell proliferation effect (%) Control 0.365 - Example 1 0.565 54.6 Example 2 0.564 54.5 Example 3 0.421 15.4 Example 4 0.480 31.4 Example 5 0.444 21.7 Example 6 0.529 44.8 Example 7 0.555 52.1 Example 8 0.496 35.9

From the above results, it can be seen that Centella extract exhibits excellent cell proliferation effect.

Experimental Example 2. Cell proliferation effect according to the concentration of Centella asiatica extract

The experiment was carried out in the same manner as in Experiment 1.

Centella asiatica extract was prepared in Example 1 and used at a different concentration. The results are as described in Table 2.

density Absorbance (570 nm) Cell proliferation effect (%) Control 0.334 - 50 µg 0.421 26.0 100 µg 0.455 36.2 200 µg 0.564 68.9

From the above results, it can be seen that the cell proliferation effect increases as the concentration of centella extract increases.

Experimental Example 3. Collagen synthesis enhancement effect of Centella asiatica extract

(1) Experimental method

Human normal fibroblasts were seeded in 96-well microplates (2 × 10 ⁴ cells / well) and incubated for 24 hours. After incubation, the medium was replaced with DM medium without serum. At this time, the centella extract of the concentration described in Table 2 was put together with the medium and incubated for 48 hours. In Table 2, the control group was cultured without adding centella extract. Centella asiatica extract used in Example 1 was used. Ascorbic acid (50 m g / ml) was added before the last 24 hours of culture to promote collagen synthesis. After incubation, each well was washed, replaced with DM medium without serum, and further incubated for 24 hours. After incubation, the supernatant of each well was collected, and the amount of procollagen type I C-piptide (PICT) was measured by the amount of collagen newly synthesized using a kit (Takara, Kyoto, Japan), and the amount of PICP was ng / Converted to 2x10 ⁴ cells.

(2) Experiment result

Table 3 shows the results of the synthesis of collagen I in human normal fibroblasts of Centella asiatica.

density Collagen Synthesis Amount (ng / 2X10 ⁴ ) Control 153 50 µg 198 100 µg 245 200 µg 291

From the above results, it can be seen that the inclusion of Centella asiatica showed better collagen synthesis enhancement effect than the control without the centellar extract, and the collagen synthesis increased as the centellar extract concentration was increased. have.

Experimental Example 4. Wrinkle improvement effect on the mouse

Five hairless mice, 6 weeks old, were used to measure the effect of improving the wrinkles of Centella asiatica on light induced wrinkles. Centella asiatica extract was applied by dissolving the Centella asiatica extract prepared in Example 1 in distilled water, and the test method is as follows.

The hairless mouse was irradiated with 2MED for 3 days a week for 10 weeks using a solar simulator to form wrinkles, and a control group treated with distilled water without adding a sample and 0.5% extract twice a day. Qualitative judgment was made in the group treated with 0.5 m / cm 2 for 6 weeks.

Wrinkle improvement was judged visually by visual inspection and photographic examination, and the criterion was no improvement, slight improvement, moderate improvement, and significant improvement compared to each extract treatment group and control group. It was determined in four steps of shown in Table 4.

No improvement Minor improvements Secondary improvement A significant improvement Control 5 0 0 0 0.5% Example 1 centella extract treatment 0 0 3 2

From the results, Centella aspergillus extract can be seen that there is an excellent improvement effect on the wrinkles generated by the light of the hairless mouse.

Experimental Example 5. Hyaluronic Acid Synthesis Enhancement Effect of Centella asiatica

Human normal fibroblasts were seeded in 6-well culture plates (3 × 10 ⁴ cells / well) and incubated for 48 hours. At this time, DM medium containing 10% fetal calf serum, penicillin, streptomycin (100 μg / ml) and the like was used. After incubation, the cells were replaced with medium containing centrifugal extracts of various concentrations and incubated for 24 hours. Centella as the extract was used in Example 1. In Table 5, the control group was cultured without adding centella extract. After incubation, only the media portion was collected and trichloroacetic acid was added to a final concentration of 10% and stored at 4 ° C. for 24 hours. The solution was centrifuged to dialyzate the supernatant, and treated with NaCl to a final concentration of 0.04 M, followed by addition of cetylpyridium chloride to precipitate hyaluronic acid. The precipitate was dissolved in 4M NaCl by centrifugation, reprecipitated in ethanol, and the precipitate was dissolved in purified water. Electrophoresis was performed using cellulose acetate strips to quantify hyaluronic acid [Hata, R. & Nagai, Y. (1972) Anal. Biochem. 45, 462-468]. Band intensities were measured, and the effect of hyaluronic acid synthesis was measured therefrom.

Hyaluronic acid synthesizing effect was calculated by the following formula, the results are shown in Table 5.

density Band Intensity Hyaluronic Acid Synthesis Enhancement Effect (%) Control 6438 - Centella asiatica extract 100 ㎍ / ㎖ 8743 35.8 Treatment of Centella asiatica 500 ㎍ / mL 10069 56.4

From the above results, it can be seen that Centella extract exhibits a hyaluronic acid synthesis-promoting effect, and as the concentration of Centella extract increases, the synthesis of hyaluronic acid in normal fibroblasts increases.

Cosmetics of the present invention by containing the centella extract, exhibits excellent cell proliferation effect, collagen and hyaluronic acid synthesis effect is excellent in preventing and improving skin aging.

Claims (7)

Cosmetic composition containing Centella asiatica extract. The cosmetic composition according to claim 1, wherein the Centella extract contains 0.0001 to 10.0 wt% based on the total dry weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the Centella extract contains 0.0005 to 10.0 wt% based on the total dry weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the Centella extract contains 0.005 to 5.0% by weight based on the total dry weight of the cosmetic composition. The method of claim 1, wherein the centella extract is 1-10 times the weight of the dry weight of the centella, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, or a mixed solvent of these lower alcohols and water, acetone, ethyl acetate, chloroform , 1,3-butylene glycol, the extract is distilled under reduced pressure and then lyophilized or spray-dried cosmetic composition characterized in that it is prepared. The cosmetic composition according to claim 1, wherein the centella extract is added to the base cosmetic selected from a flexible lotion, a nourishing lotion, a massage cream, an essence, a pack, and the like. The cosmetic composition according to claim 1, which can be used for the purpose of preventing and improving skin aging.