KR20010018289A - Kit for diagnosing pregnancy - Google Patents

Abstract

PURPOSE: A pregnancy diagnosis kit is provided to readily decide the pregnancy of a cow 19 or 20 days after fertilization by using the concentration of progesterone existing in blood. CONSTITUTION: A thin film type paper(1) forms a specific size of pores inside. A progesterone-BSA conjugate(2) of specific concentration is coated in a specific area on at least one face of the paper. A colloidal gold label(3) embedding anti-progesterone is combined with some part of an upper face of the progesterone-BSA conjugate. The concentration of progesterone existing in blood, progesterone-cow serum albumin fixed on a thin film and an anti-progesterone gold conjugate are competitively reacted. Color reaction is performed in reverse proportion to the progesterone concentration in the blood. Accordingly, the pregnancy of the cow is readily decided.

Description

Pregnancy Diagnostic Kit {KIT FOR DIAGNOSING PREGNANCY}

The present invention relates to a pregnancy diagnosis kit, and more particularly, to a pregnancy diagnosis kit that makes it possible to easily determine whether or not a Korean cow is pregnant using progesterone (progesterone) concentration present in the blood.

Prior to the 1990s, veterinarians rely solely on the method of judging the feelings of a Korean cattle by rectal examination 50 to 60 days after fertilization or mating. However, the method requires a skilled artificial insemination or a veterinarian when the pregnancy is assessed by rectal examination 50 to 60 days after fertilization, and caused a lot of economic losses due to the long periods of Hanwoo cow, for example, Promoting ovaries and fetuses to the rectum during pregnancy can not exclude the possibility of ovarian disease and miscarriage, and could stress Korean cattle.

In addition, since the above method can determine whether or not a certain pregnancy when 50 to 60 days after fertilization, if it is judged that the pregnancy is not at this time, check the estrus signs after 60 days and then correct the procedure again There was a hassle to go through.

In relation to this, since the 1990s, the development of science has been developing methods to evaluate the fertility, early pregnancy and reproductive disorders of Korean cattle. For example, pregnancy diagnosis using ultrasonic diagnostic apparatus is mainly used. Because it is often used by non-experts in the industry, mechanical robustness and the possibility of early diagnosis, convenience of diagnosis, low misdiagnosis rate, low maintenance cost, and convenience of equipment operation are required.

Thus, a recently developed kit (kit, aka Baekdu Prokit), which can diagnose pregnancy at a concentration of progesterone in the blood, has been developed and marketed.

However, the Baekdu Prokit is capable of diagnosing pregnancy if it is about 19 to 22 days after fertilization, but simple but misdiagnosis occurs according to the strength of enzyme activity, and because the test process consists of about 10 steps, There was a problem that could not diagnose pregnancy.

The present invention was devised to solve the above problems, using the concentration of progesterone (progesterone) present in the blood can be easily determined early 19 or 20 days after fertilization of the Hanwoo fertility and the same individual The aim is to provide a pregnancy diagnostic kit designed to be repeated.

1 is a plan view showing the configuration of a pregnancy diagnosis kit according to the present invention.

Figure 2 illustrates the principle of color development according to the pregnancy diagnostic kit according to the present invention

drawing.

<Explanation of symbols for main parts of drawing>

1: Membrane Paper 1a: Porous

2: progesterone layer 3: colloid label layer

4: progesterone present in the sample

5: progesterone gold conjugate

Pregnancy diagnostic kit according to the present invention for achieving the above object is made of a thin-film paper with a predetermined size of pores therein, the progesterone layer bonded to bovine serum albumin on at least one side of the paper to form a predetermined region coating The colloidal label layer in which the anti-progesterone antibody is embedded is coupled to a part of the upper surface of the progesterone layer.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a plan view showing the configuration of a pregnancy diagnosis kit according to the present invention, Figure 2 is a view showing the principle of color development according to the pregnancy diagnosis kit according to the present invention.

As shown, the exterior is made of thin film 1 having pores having a predetermined diameter of 8 to 12 µm.

The thin film 1 is preferably made of a material capable of absorbing a sample, for example, a fluid such as blood or milk, and the reason for limiting the diameter of the pore to 8 to 12 μm is that blood is the paper 1. This is to control the absorption and moving speed by capillary phenomenon when buried in the surface and gradually absorbed into the surface.

In other words, it is to control the reaction time of the test by adjusting the rate of passage of the sample, that is, the components necessary for the reaction, the steroid hormone in the blood components.

A progesterone layer (progesterone-BSA conjugate) 2 is formed on at least one surface of the paper 1 by coating a predetermined region.

On the upper part of the antiprogesterone layer 2, a gold colloid label 3 incorporating an antiprogesterone antibody (monoclonal anti-progesterone) is bound.

Referring to the operation of the pregnancy diagnostic kit according to the present invention configured as described above and the process of pregnancy is as follows.

First, a drop of serum (0.04 mL) and a drop of antiprogesterone gold colloid conjugate (0.04 mL) collected at 19, 20 and 21 days after artificial insemination from Korean cattle were mixed in a test tube, and the test strip was dropped. The steroid hormone and the gold colloid label are introduced through the pores 1a formed inside the paper 1 while being sucked into the strip.

Subsequently, when the steroid hormone in the blood binds to the gold colloidal label and gradually reaches a fixed position of the progesterone, the progesterone layer contained in the sample and the progesterone layer immobilized on the thin film are competitively bonded, resulting in the portion having the progesterone layer. The color is bright red, which is inversely proportional to the amount of progesterone contained in the steroid hormone.

Therefore, the user (stocker) can determine whether the livestock is pregnant or non-pregnant by comparing the color development of the crimson color of the strip with the standard solution relatively.

As described above, according to the pregnancy diagnosis kit according to the present invention, it is possible to easily determine whether or not a Korean cow is pregnant using progesterone concentrations in the blood.

Claims (4)

It is made of thin film paper formed with a predetermined size of pores therein, Pregnancy diagnosis kit, characterized in that the anti-progesterone layer is formed on at least one side of the paper is coated with a predetermined area, and the colloidal label layer embedded with anti-progesterone (monoclonal anti-progesterone) is bonded to a portion of the upper surface of the anti-progesterone layer. The method of claim 1, The size of the pores of the thin film type pregnancy diagnosis kit, characterized in that 8 to 12㎛ to enable the movement of the sample by the capillary phenomenon. The method of claim 1, The label layer is a pregnancy diagnostic kit, characterized in that the colloidal gold and the antibody is coupled. The method of claim 1, A pregnancy diagnostic kit characterized by an immunoassay in which a natural progesterone and a synthetic progesterone in a sample react competitively with a marker conjugated with a colloidal gold monoclonal antibody.