KR20000000757A - Composition of herb medicine for promting release of growth hormone and promoting agent for release of growth hormone using the same - Google Patents

Abstract

PURPOSE: A composition of herb medicine for promoting release of growth hormone is provided which has no adversary side effect compared with conventional promoting agent for release of growth hormone. CONSTITUTION: The composition comprises: 15-46 wt% of steamed Rehmannia glutinosa; 5-36 wt% of Cornusofficinalis; 5-36 wt% of white agaric; 4-35 wt% of Alisma canaliculatum; 3-34 wt% of the root of Lycium chinense, Scrophularia buergeriana, the fruit of Lycium chinense, Rubus schizostulus, Psyllium, Schizonepeta tenuifolia var. japonica, Ledebourielleseseloides, the soft core of the young antlers of the deer, a deer's horn, Cordycepssinensis, Anemarrhena asphodeloides and Phellodendron amurense respectively. A promoting agent for release of growth hormone is obtained by: mixing the components; extracting; concentrating under reduced pressure followed by cooling; making 65% ethanol solution by adding ethanol; agitating followed by filtering; concentrating again under reduced pressure followed by cooling; making 85% ethanol solution by adding ethanol; agitating followed by filtering; concentrating again under reduced pressure to evaporate the ethanol; freeze-drying; dissolving it in physiological salt solution; and filtering with milipore filter paper.

Description

Herbal composition for promoting growth hormone secretion and growth hormone secretagogue prepared using the same

The present invention relates to a herbal composition for promoting growth hormone secretion that can induce growth by promoting growth hormone secretion without side effects, and a growth hormone secretagogue prepared by using the same, and more specifically 15 to 46% by weight of sulfur , Cornus and Baekbokryeong each 5 to 36% by weight, taeksa and the neck skin 4 to 35% by weight, phalanx skin, Hyunsam, Gugija, Bokbunja, tea electrons, mold opening, windproof, deer antler, green rust, Cordyceps sinensis, 3-4% It is composed of%, and each powder is uniformly mixed with powder, precipitated and extracted with distilled water, filtered, concentrated under reduced pressure and cooled, added with ethanol to make 65% ethanol solution, stirred and left at low temperature to precipitate. After filtration, the mixture was concentrated and cooled by secondary pressure reduction, ethanol was added to make an 85% ethanol solution, stirred, and left to stand at low temperature to filter the precipitate. Um, tertiary reduced pressure, distilled water is added, concentrated under reduced pressure, ethanol is completely evaporated, lyophilized, dissolved in physiological saline, filtered through Millipore filter paper, and prepared as an injection solution. It relates to a composition and a growth hormone secretagogue prepared using the same.

As the dietary growth improved with economic growth, the average height of adolescents increased significantly compared to 10 years ago. However, in some cases, there are not many cases where growth is hindered by not only genetic factors but also acquired diseases.

Therefore, for the treatment of dwarfism as described above, a method of administering a growth hormone produced in large quantities by genetic engineering techniques or physically increasing a certain portion of bone by surgery or the like is used.

However, clinical results by these methods, which are prescribed only before or after growth arrest, are sometimes seriously adverse, and the expected effect is not great and the economic burden is not small.

In general, growth hormone secreted from the anterior pituitary is also called somatotropin, which promotes the growth and development of the body and directly acts on the metabolism of sugars, fats and proteins, resulting in skeletal growth as well as intestines. It exhibits various actions to regulate the growth rate of.

In addition, the concentration of growth hormone in the blood rises rapidly in a few minutes due to stimulation such as sleep or stress. Somatomedin, which is produced in the kidney or liver by the action of growth hormone, is the collagen of chondrocytes. Promote synthesis to grow bone.

The rapid growth during puberty is accompanied by increased secretion of growth hormones and sex hormones. Growth hormone helps the growth of long bones, sex hormone helps the growth of the ulna, and growth hormone secretion is known to be a rapid growth, growth hormone secretion of the growth hormone (Growth hormone) growth hormone release hormone (Growth of the hypothalamus) Hormone Releasing Hormon (GHRH) and is inhibited by growth hormone secretion inhibitor hormone (somatostatin (Somatostatin (SS))). In addition, substances that stimulate or inhibit the secretion of growth hormone known to date are mostly known to act on the hypothalamus to indirectly regulate the secretion of growth hormone by controlling the secretion of GHRH and SS.

Since 1958, growth hormone has been administered to patients with growth hormone deficiency. As growth hormone is mass-produced by the development of genetic engineering, growth hormone non-deficiency dwarfism such as Turner syndrome, familial dwarfism, as well as growth hormone deficiency In some cases, growth hormone may be administered.

However, growth hormone preparations have side effects such as hip dislocation, hypothyroidism, and growth hormone antibody formation.Glucose intolerance may occur when administered in large doses, and therefore, it is necessary to prevent abuse of growth hormone. Without this, it is urgent to develop a treatment that naturally induces growth.

Accordingly, it is an object of the present invention to provide a herbal composition for promoting growth hormone secretion that can induce growth by promoting growth hormone secretion without side effects.

Another object of the present invention to provide a growth hormone secretagogue prepared by using the herbal composition for promoting growth hormone secretion of the above object.

In order to achieve the above object as well as another object that can be easily expressed in the present invention, 15 to 46% by weight of Sukjihwang, 5 to 36% by weight of cornus and Baekbokryeong, 4 to 35% by weight of taxa and neck skin, phalanges, It consists of 3 ~ 34% by weight of Hyunsam, Gugija, Bokbunja, Chajeon, Hyeonggae, Windproof, Deer Antler, Green Ginseng, Cordyceps Sinensis, Zimo, and Yellow White, and then mix the powders uniformly and settle into distilled water. After filtration and concentration, the mixture was concentrated under reduced pressure and cooled, added to ethanol to form a 65% ethanol solution, stirred, and left to stand at low temperature. The precipitate was filtered off, and then concentrated under reduced pressure to cool, and ethanol was added to make a 85% ethanol solution. The mixture was left at low temperature, filtered, and the precipitate was filtered, and then concentrated under reduced pressure. Then, distilled water was added and concentrated under reduced pressure to completely evaporate ethanol, followed by freeze drying and physiological salt. Next, the mixture was filtered through a Millipore filter paper dissolved in the producing a growth hormone secretagogue in injection solutions and this effect could be obtained that can promote the growth hameuroseo administered in growing children or dwarfism patients.

1 and 2 are graphs showing the growth hormone content measured in Example 1.

The present invention is described in more detail as follows.

The herbal composition for promoting growth hormone secretion according to the present invention is 15 to 46% by weight of Sukjihwang, 5 to 36% by weight of Cornus and Baekbokryeong, 4 to 35% by weight of Taeksa and Magnolia skin, Jigolpi, Hyunsam, Gugijak, Bokbunja, Cha-jeon, It is characterized by consisting of 3 to 34% by weight of mold opening, windproof, deer antler, green shell, cordyceps sinensis, hair loss and yellowish white.

In addition, the growth hormone secretagogue according to the present invention 15 to 46% by weight of sukjihwang, 5 to 36% by weight of corn and baekbokryeong, 4 to 35% by weight each of talc and neck skin, Jigolpi, Hyunsam, Gugija, Bokbunja, tea and mold , Windproof, Deer Antler, Green Ginseng, Cordyceps Sinensis, Balaxia, and Yellow White, each composed of 3 to 34% by weight, each of the components are finely mixed and mixed with powder, precipitated and extracted with distilled water, filtered and concentrated under reduced pressure. Ethanol was added to make a 65% ethanol solution, stirred and left to stand at low temperature to filter the precipitate. The precipitate was concentrated and cooled. Secondary pressure reduction was added to make 85% ethanol solution. The mixture was stirred and left at low temperature to filter the precipitate. , Concentrated under reduced pressure, distilled water was added, concentrated under reduced pressure, ethanol was completely evaporated, lyophilized, dissolved in physiological saline, and then filtered through Millipore filter. It is characterized by producing a liquid.

Sukjihwang is the dried root of dried Guhwang (Rehmannia glutinosa) belonging to the Hyunsam family, which has the effect of consonant, bowie, blood, landscaping, etc., and has been used in various medicines from the traditional medicine. In the present invention, the amount of sulfur used in the present invention is 15 to 46% by weight, and when the amount is less than 15% by weight, the effect of addition is insignificant. There is a problem that cannot achieve the desired effect.

Cornus is dried fruit or seed of cornus tree, it is widely used as antipyretic, tonic, low back pain, relieves, diuretic, etc., the amount of use in the present invention is effective 5 to 36% by weight, the amount is less than 5% by weight In the case of the addition effect is weak, when the amount exceeds 36% by weight relatively has a disadvantage that the amount of the other components are reduced to achieve the effect to be obtained in the present invention. Baekbokryeong is a kind of fungus belonging to the family of fungi, which are parasitic in the pine family. They are parasitic and grow on rotten trees that have cut pine trees. 5 to 36% by weight of the effective amount of, when the amount is less than 5% by weight of the effect of the addition is weak, when the amount exceeds 36% by weight of the other components of the relatively reduced amount of the effect to obtain in the present invention There is a disadvantage that can not be achieved.

Taxa is a perennial aquatic herb that grows about 50 ~ 100cm and is a diameter of the plantain of the planar tree belonging to the celtic family whose leaves are spherical. To dry in the sun, or to use it on low heat, use diuretic, branch, moist heat, strong blood, and use 4 to 35% by weight, if the amount is less than 4% by weight, the effect of addition If the amount is less than 35% by weight, the amount of the other components is relatively reduced, so that the effect to be obtained in the present invention cannot be achieved.

Bark skin is the root of Peony (Paeonia suffruticosa) belonging to the perennial deciduous subtilis, Peaonia suffruticosa, collected and washed to remove fine roots and stems, and then to remove the core and dried in the sun. It is known to have an effect such as anti-inflammatory, and in the present invention, it is effective to use 4 to 35% by weight, and when the amount of use is less than 4% by weight, the effect of addition is weak, and when it exceeds 35% by weight, There is a disadvantage in that the amount of other components used is reduced to achieve the effect desired in the present invention.

Phalanx is a deciduous shrub that is a root of the Goji berry tree belonging to the branch family, and has the efficacy of self, interpolation, rotation, consonant, nominal, etc. Hyunsam is a perennial herb and is collected as the root of Scrophularia ningpoensis belonging to the Hyunsam family Remove stems and fine roots, clean them, and dry them in the sun for about 5 days. They are effective for consonant, fortification, cultivation, and detoxification. Goji is collected as mature fruit of Lycium chinense, which belongs to the branch family. After drying in a well-winded shade, and then dried in the sun, using the self, interpolation, rotation, consonant, nominal effect.

Bokbun is the fruit of bokbunja strawberry and is used in the stomach and urinary incontinence. The tea is used as a diuretic because of its nature as a seed of plantain. It is also used as a medicine for eye disease, diarrhea and cough. As an outpost of Schizonepeta tenuifolia belonging to the family, it is used when autumn flowers bloom and the blue energy of the plant is collected, dried in the sun and the flower parts are removed. Efficacy in sweating, fever, wind, pain, and hemostasis It has been applied to chills, fever, rubella, etc.

Windproof is a perennial herb that is harvested as the root of windproof belonging to the Apiaceae, and removes the remaining stems and roots, cleans the soil, and dry it in the sun.It has the effects of detoxification, sweating, wind, dehumidification, pain relief, It is frequently applied to wind sickness, neuralgia, arthritis, etc., and the deer antler has a new tender horn of deer, which has the power to help blood and strengthen the heart.

Deer antler is used as a medicinal herb as a deer's horn, which reduces the amount of blood and stiff hairs. The cordyceps is a mushroom that grows in the chrysalis of various insects such as silkworm chrysalis. It is known that it is a perennial herb that is collected from the root of Anemarrhena asphodeloides belonging to the genus and removes rhizome roots, and then dried in the sun. Is a deciduous arboreous tree, which is the root of Phellodendron amurense, belonging to the Unhyang family, and is dried first in the sun, taking care not to peel off the bark and flattening it. It is effective in antipyretic, humid, dead, sterilization, dysentery, small brown, hepatitis, jaundice and red white. And, the application purpose, stomatitis, hwalyeom, swelling or the like.

Gigolpi, Hyunsam, Gugija, Bokbunja, Cha-jeon, Hyeonggae, Windproof, Deer Antler, Green Ginseng, Cordyceps Sinensis, Zimo, and Yellow White, respectively, are effective to use 3 to 34% by weight, and the amount of addition is less than 3% by weight. And, if it exceeds 34% by weight, the amount of other components is relatively reduced, there is a problem that can not achieve the effect to be obtained in the present invention.

Prepare the growth hormone secretagogue of the present invention with the above components, but finely mix the powder by grinding each component, precipitated and extracted with distilled water, filtered and concentrated under reduced pressure, cooled and added ethanol 65% Ethanol was made into a solution, stirred and left to stand at low temperature, the precipitate was filtered. Secondary pressure was concentrated and cooled. Then, ethanol was added to make 85% ethanol solution. The mixture was stirred and left at low temperature to filter the precipitate. After distilled water was added and concentrated under reduced pressure, ethanol was completely evaporated, lyophilized, dissolved in physiological saline, and then filtered through Millipore filter paper to prepare an injection solution.

The finer the powder is, the more effective the active ingredient can be easily extracted from each component. However, economically, it is effective to be about 320 mesh.

Pour each of the ingredients and mix the powder evenly into a Erlenmeyer flask, add 1000 ml of distilled water, precipitate for 24 hours in a shaking incubator, filter, and then shake the remaining residue with 500 ml of distilled water. Precipitate and filter for 24 hours in a shaking incubator, combine the first extract, concentrate under reduced pressure to make the residue 50ml, cool to room temperature, add ethanol to make 65% ethane solution, stir and cool The precipitate was filtered for 24 hours, filtered, and the filtrate was concentrated under a reduced pressure to 50 ml. The residue was cooled to room temperature, cooled to room temperature, ethanol was added to make an 85% ethanol solution, stirred, and the precipitate was filtered at room temperature. Next, concentrate under reduced pressure to make the remaining amount 50ml, add 50ml of distilled water, and depressurize again. And the remaining amount so that chukhayeo 50ml and then completely evaporated ethanol, lyophilized, and the dried extract was filtered as dissolved in physiological saline solution and Millipore filter paper to produce a growth hormone secretagogue by intraperitoneal injection injectable form.

When the composition for promoting growth hormone secretion of the present invention as described above is extracted in a well-known method to drink for a long time to dwarfism patients or injection of a growth hormone secretagogue prepared as an injection into the abdominal cavity, the secretion of growth hormone is promoted In addition to growth hormone deficiency, it is possible to promote the growth of patients with growth hormone non-deficient dwarfism such as Turner syndrome and familial dwarfism.

Although the composition or growth hormone secretagogue of the present invention is not known mechanism of action, it has been found that the administration of such composition or growth hormone secretagogue to treat dwarfism in patients with other symptoms. In particular, the compositions or growth hormone secretagogues of the present invention were effective in patients with dwarfism who had the nature of Soyangin in filamentous constitution.

The following examples illustrate the invention in more detail, but do not limit the scope of the invention.

Example 1

16g of sujijihwang, 8g each of cornus and Baekbokryeong, 6g each of talc and velvet skin, Jigolpi, Hyunsam, Gugija, Bokbunja, tea electron, hyungae, windbreak, deer antler, green rust, Cordyceps sinensis, jimo and yellow white 4g were put into the grinder and pulverized. After mixing and uniformly mixing, weigh 20 g into a Erlenmeyer flask, add 100 ml of distilled water, precipitate for 24 hours in a shaking incubator, filter, and then add 500 ml of distilled water to the remaining residue again. The precipitate was extracted for 24 hours in a shaking incubator, filtered and the first extract was combined, concentrated under reduced pressure until the remaining amount was 50 ml, cooled to room temperature, added to ethanol to a 65% ethanol solution, stirred, and stirred for 24 hours at low temperature. The precipitate was filtered off, and the filtrate was concentrated under a second reduced pressure so that the remaining amount was 50 ml. Add Ole to make 85% ethanol solution, stir and leave at low temperature to filter the precipitate. Concentrate under reduced pressure to make the residue 50ml, add 50ml of distilled water and concentrate under reduced pressure to make 50ml. After ethanol was completely evaporated, lyophilized, the dried extract was dissolved in physiological saline, and then filtered through Millipore filter paper to prepare a growth hormone secretagogue of the present invention in the form of an injection solution for intraperitoneal injection.

Efficacy was evaluated by administering the prepared growth hormone secretagogue to the rat.

In other words, after selecting the rat (Sprague-Dawley, male) as the experimental animal, the birth date was the first day of breeding at the standard conditions (22 ± / ℃, humidity 55 ± 5%) of 21 days of age 25 ~ 30g of body weight Rats were selected, and each group was made of 7 to 8 rats. The growth hormone secretagogues 1.0g / kg and 10.0g / kg were respectively diluted in physiological saline and then intraperitoneally injected into the rats. After 15 minutes, anesthesia was performed with ether, blood was collected from the heart, serum was separated (3,000 rpm, 4 ° C.), and the growth hormone kit (Growth Hormone kit) was used for the radioimmuno assay (Radioimmuno Assay). The growth hormone content of the serum was measured by quantification using a counter (Packard, Auto Gamma 5550).

As a control, growth hormone secreting peptide (GHRP) _D- YS-TYR _{-D -} TRP- _{D -} TRP-PHE AMIDE (L4135) was purchased from Sigma Chemical Co. (St, Louis, Mo). The growth hormone content was measured by injection into the abdominal cavity in the same manner as the growth hormone secretagogue.

In addition, in the above Example, all the reagents were purchased from Aldrich.

The measured growth hormone content is shown in Table 1, Figure 1 and Figure 2.

Table 1

a): mean ± standard deviation

NS: normal saline

GR: GHRP

SA: Growth hormone secretagogue of the present invention

As can be seen from Table 1, the growth hormone content in the saline solution used as a control group was 38.6 ± 4.04 pg / ml, and the growth hormone secretagogue of the present invention was intraperitoneally injected with 1.0 g / kg and 10 g / kg. Experimental groups were 42.9 ± 5.22 pg / ml and 45.0 ± 4.63 pg / ml, respectively, to promote growth hormone secretion.

Example 2

50 male students aged 10-15 years old who are determined to be Taeumin by Euiin and dryness 12g, Nagjaja 6g, Gilgyeong, Baekmun-dong, Schisandra, Seokchangpo, Wonji, Cheonmun-dong, Sanjoin, Yonganyuk, Nokyong, Nokgak, Baekgangjam , 4 g each of ripen and rhubarb are added to a grinder, pulverized and finely powdered, mixed uniformly, and weighed 20 g into a Erlenmeyer flask, and 1000 ml of distilled water is added to precipitate and extract for 24 hours in a shaking incubator. After filtration, 500 ml of distilled water was added to the remaining residues again, and the precipitate was extracted for 24 hours in a shaking incubator, filtered, the first extracts were combined, concentrated under reduced pressure to 50 ml, and then cooled to room temperature. Ethanol was added to make 65% ethanol solution, stirred and left at low temperature for 24 hours to filter the precipitate, and then the filtrate was washed with 2 Concentrate under reduced pressure to make the remaining amount 50ml, then cool to room temperature, add ethanol to make 85% ethanol solution, stir and leave at low temperature to filter the precipitate, and then concentrate under reduced pressure to make the remaining amount to 50ml. 50ml of distilled water was added and concentrated under reduced pressure to make the remaining amount 50ml. The growth hormone secretagogue, which was completely evaporated with ethanol, was administered for 6 months to 1 year. In the case of 5-9cm growth, 20 people were 40%, and 10-15cm growth was 15 people, 30%, and more than 15cm growth was 1 person, 2%.

In the meantime, the average growth rate of adolescents during the growth period shown by the National Institute of Technology and Quality in 1997 was 3.5cm, and when the growth hormone secretion stimulant of the present invention was grown above the average value, 72% of the growth occurred. It was found to be effective.

As described above, in the present invention, 15 to 46% by weight of Sukjihwang, 5 to 36% by weight of cornus and Baekbokryeong, 4 to 35% by weight of talc and velvet skin, phalanx, hyeonsam, wolfberry, bokbunja, tea, mold, windproof and antler It consists of 3 ~ 34% by weight of green tea, Cordyceps sinensis, hair loss, and yellowish white powder. Mix each powder uniformly, precipitate precipitate in distilled water, filter, concentrate under reduced pressure, cool and add ethanol. It was made into 65% ethanol solution, stirred and left to stand at low temperature to filter the precipitate. The precipitate was concentrated and cooled by secondary pressure reduction, ethanol was added to make it into 85% ethanol solution, stirred, and left to stand at low temperature to filter the precipitate. Concentrated, distilled water was added, concentrated under reduced pressure, ethanol was completely evaporated, lyophilized, dissolved in physiological saline, filtered through millipore filter paper, and grown into injection solution. By preparing a lemonium secretion accelerator and administering it to a growing child or dwarf patients, it was possible to obtain the effect of promoting growth.

Claims (3)

Hwangji Hwang 15 to 46% by weight, cornus and 5 to 36% by weight of Baekbokryeong, 4 to 35% by weight of talc and vellum, Jigolpi, Hyunsam, Gugija, Bokbunja, teapot, mold opening, windproof, deer antler, green rust, cordyceps, jimo and Growth hormone secretagogue composition, characterized in that composed of 3 to 34% by weight of each white. Hwangji Hwang 15 to 46% by weight, cornus and 5 to 36% by weight of Baekbokryeong, 4 to 35% by weight of talc and vellum, Jigolpi, Hyunsam, Gugija, Bokbunja, teapot, mold opening, windproof, deer antler, green rust, cordyceps, jimo and It consists of 3 to 34% by weight of each white-white powder, and mixes the powders uniformly, precipitates and distills into distilled water, filters, concentrates under reduced pressure, cools, adds ethanol to make 65% ethanol solution, and stirs them. The mixture was left at low temperature, filtered, and the precipitate was concentrated and cooled by secondary depressurization, cooled to 85% ethanol solution by adding ethanol, stirred, and left to stand at low temperature to filter the precipitate. Concentrated and completely evaporated ethanol, lyophilized, dissolved in physiological saline, and filtered by Millipore filter paper to produce a growth hormone, characterized in that the injection solution Secretagogues. The growth hormone secretagogue according to claim 2, characterized in that it is applied to Soyangin in filamentous constitution.