KR102905512B1 - 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법 - Google Patents

조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Info

Publication number
KR102905512B1
KR102905512B1 KR1020237040139A KR20237040139A KR102905512B1 KR 102905512 B1 KR102905512 B1 KR 102905512B1 KR 1020237040139 A KR1020237040139 A KR 1020237040139A KR 20237040139 A KR20237040139 A KR 20237040139A KR 102905512 B1 KR102905512 B1 KR 102905512B1
Authority
KR
South Korea
Prior art keywords
delete delete
engineered
glycosyltransferase
seq
polypeptide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020237040139A
Other languages
English (en)
Korean (ko)
Other versions
KR20230162160A (ko
Inventor
조나단 브룸
스테파니 수 가라니
니키 델라스
잭 리앙
조이스 리우
데이비드 엔트위슬
코트니 다이앤 모펫
Original Assignee
테이트 앤드 라일 솔루션스 유에스에이 엘엘씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 filed Critical 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨
Priority to KR1020257042943A priority Critical patent/KR20260006703A/ko
Publication of KR20230162160A publication Critical patent/KR20230162160A/ko
Application granted granted Critical
Publication of KR102905512B1 publication Critical patent/KR102905512B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
KR1020237040139A 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법 Active KR102905512B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020257042943A KR20260006703A (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201762454417P 2017-02-03 2017-02-03
US62/454,417 2017-02-03
US201762479262P 2017-03-30 2017-03-30
US62/479,262 2017-03-30
PCT/US2018/016359 WO2018144679A2 (en) 2017-02-03 2018-02-01 Engineered glycosyltransferases and steviol glycoside glucosylation methods
KR1020197025642A KR102606350B1 (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020197025642A Division KR102606350B1 (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020257042943A Division KR20260006703A (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Publications (2)

Publication Number Publication Date
KR20230162160A KR20230162160A (ko) 2023-11-28
KR102905512B1 true KR102905512B1 (ko) 2025-12-29

Family

ID=63038689

Family Applications (3)

Application Number Title Priority Date Filing Date
KR1020237040139A Active KR102905512B1 (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
KR1020197025642A Active KR102606350B1 (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
KR1020257042943A Pending KR20260006703A (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020197025642A Active KR102606350B1 (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
KR1020257042943A Pending KR20260006703A (ko) 2017-02-03 2018-02-01 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법

Country Status (15)

Country Link
US (5) US20180223264A1 (https=)
EP (3) EP3577229A4 (https=)
JP (4) JP7248302B2 (https=)
KR (3) KR102905512B1 (https=)
CN (2) CN110914445B (https=)
AU (4) AU2018215335B2 (https=)
BR (1) BR112019015992A2 (https=)
CA (3) CA3175100A1 (https=)
IL (3) IL304833B2 (https=)
MX (4) MX391104B (https=)
MY (1) MY199186A (https=)
PH (1) PH12019501698A1 (https=)
SG (1) SG11201906480QA (https=)
TW (3) TW202237833A (https=)
WO (2) WO2018144675A1 (https=)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102905512B1 (ko) 2017-02-03 2025-12-29 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
AU2017402700A1 (en) * 2017-03-06 2019-10-17 Conagen Inc. Biosynthetic production of steviol glycoside rebaudioside D4 from rebaudioside E
MY204735A (en) * 2018-07-16 2024-09-11 Manus Bio Inc Production of steviol glycosides through whole cell biotransformation
IL280302B2 (en) 2018-07-30 2025-09-01 Codexis Inc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside
CN111378631B (zh) * 2019-01-28 2021-08-24 江南大学 一种海藻糖合成酶突变体及其在海藻糖生产中的应用
EP3973069A4 (en) * 2019-05-23 2023-09-13 Arzeda Corp. COMPOSITIONS AND METHODS FOR PRODUCING STEVIOL GLYCOSIDES
WO2020264179A1 (en) * 2019-06-25 2020-12-30 Manus Bio, Inc. Uridine diphosphate-dependent glycosyltransferase enzyme
CN112553175B (zh) * 2019-09-26 2023-04-07 中国科学院分子植物科学卓越创新中心 糖基转移酶ugt76g1突变体的制备及其应用
WO2021126960A1 (en) * 2019-12-16 2021-06-24 Manus Bio, Inc. Microbial production of mogrol and mogrosides
CN111662942A (zh) * 2020-05-25 2020-09-15 安徽金禾实业股份有限公司 一种双酶发酵催化生产莱鲍迪苷a的方法
EP4176070A1 (en) 2020-07-03 2023-05-10 c-LEcta GmbH One-pot cell-free glycosylation process
US20250290081A1 (en) 2020-10-22 2025-09-18 Dsm Ip Assets B.V. Microorganisms for diterpene production
KR102546737B1 (ko) * 2020-11-25 2023-06-22 씨제이제일제당 주식회사 포도당 전이 스테비아를 포함하는 감미질이 개선된 조성물
CN114480326B (zh) * 2021-01-08 2024-04-26 中国科学院华南植物园 亚精胺衍生物糖基转移酶LbUGT73及其编码基因和应用
CN112704731B (zh) * 2021-01-13 2023-08-25 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 蛋白酶s273r抑制细胞焦亡的用途及方法
EP4286515A4 (en) * 2021-01-27 2026-02-25 Korea Advanced Inst Sci & Tech VARIANTS OF C-GLYCOSYLTRANSFERASE AND THEIR USE
WO2022253282A1 (zh) * 2021-06-01 2022-12-08 弈柯莱生物科技(上海)股份有限公司 一种糖基转移酶及其应用
CN115449514B (zh) * 2021-06-08 2023-09-29 弈柯莱生物科技(上海)股份有限公司 一种β-1,2-糖基转移酶及其应用
CN115678867B (zh) * 2021-07-27 2023-12-01 弈柯莱生物科技(集团)股份有限公司 一种蔗糖合成酶及其应用
CN113462670B (zh) * 2021-08-23 2023-03-24 兴化格林生物制品有限公司 一种糖基转移酶突变体及其催化合成莱鲍迪苷m的方法
WO2023044368A1 (en) * 2021-09-17 2023-03-23 Doublerainbow Biosciences Inc. Method for producing glycosylated therapeutics by using an immobilized enzyme preparation
MX2024004833A (es) * 2021-10-19 2024-05-03 Cj Cheiljedang Corp Procedimiento para producir rebaudiosido d y rebaudiosido m.
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
US20250051818A1 (en) * 2021-12-24 2025-02-13 Samyang Corporation Production of rebaudioside
CA3266431A1 (en) * 2022-09-02 2024-03-07 Arzeda Corp. Compositions and methods for producing rebaudioside m
EP4608984A2 (en) * 2022-10-26 2025-09-03 Arzeda Corp. Enzymatically mediated reactive crystallization of steviol glycosides
CN120858181A (zh) 2022-12-05 2025-10-28 泰特和莱尔解决方案美国公司 获得甜菊醇糖苷的方法
CN116426506B (zh) * 2023-03-10 2024-01-30 云南师范大学 低温活性提高的β-木糖苷酶突变体D259G及其应用
EP4724575A1 (en) * 2023-06-06 2026-04-15 Arzeda Corp. Compositions and methods for producing rebaudioside d and rebaudioside m
CN119432785B (zh) * 2023-08-07 2026-02-06 中国科学院天津工业生物技术研究所 一种高丝氨酸脱氢酶突变体及其应用
WO2025039672A1 (zh) 2023-08-24 2025-02-27 弈柯莱生物科技(集团)股份有限公司 莱鲍迪苷M的制备方法和β-1,2-葡萄糖基转移酶在其中的应用
CN119931981A (zh) * 2024-05-29 2025-05-06 东台市浩瑞生物科技有限公司 一种糖基转移酶ugt76g1突变体及其催化合成莱鲍迪苷a的方法
WO2026063396A1 (ja) * 2024-09-20 2026-03-26 富士フイルム株式会社 変異型スクロースシンターゼ、変異型スクロースシンターゼをコードする核酸、発現ベクター、形質転換体、変異型スクロースシンターゼの製造方法、変異型スクロースシンターゼを含む組成物、ウリジン二リン酸－グルコースの製造方法、糖化合物の製造方法
CN120384061B (zh) * 2025-04-23 2026-03-13 杭州力文所生物科技有限公司 一种用于催化生产莱鲍迪苷m的糖基转移酶及其重组基因
CN121718520A (zh) * 2026-02-26 2026-03-24 山东奔月生物科技股份有限公司 β-1,3-糖基转移酶突变体及其在催化合成莱鲍迪苷M中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
JP2016506739A (ja) 2013-02-06 2016-03-07 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． レバウディオサイドｄおよびレバウディオサイドｍの改良された産生方法

Family Cites Families (184)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58149697A (ja) 1982-02-27 1983-09-06 Dainippon Ink & Chem Inc β−１，３グリコシルステビオシドの製造方法
JP3255409B2 (ja) 1990-11-29 2002-02-12 キヤノン株式会社 画像形成装置
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO1995033836A1 (en) 1994-06-03 1995-12-14 Novo Nordisk Biotech, Inc. Phosphonyldipeptides useful in the treatment of cardiovascular diseases
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6265201B1 (en) 1997-01-17 2001-07-24 Regents Of The University Of Minnesota DNA molecules and protein displaying improved triazine compound degrading ability
EP1717322B1 (en) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
IL138800A0 (en) 1998-04-02 2001-10-31 Tellus Genetic Resources Inc A method of identifying a plant with a lesion in a gene sequence
NZ507591A (en) 1998-05-01 2002-03-28 Maxygen Inc Optimization of pest resistance genes using DNA shuffling
WO1999065927A2 (en) 1998-06-17 1999-12-23 Maxygen, Inc. Method for producing polynucleotides with desired properties
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
WO2000009682A1 (en) 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
EP1130093A1 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
JP2003524394A (ja) 1999-02-11 2003-08-19 マキシジェン， インコーポレイテッド ハイスループット質量分析法
CA2362737A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6703240B1 (en) 1999-04-13 2004-03-09 Maxygar, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
IL150291A0 (en) 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
JP2004508011A (ja) 2000-04-03 2004-03-18 マキシジェン，　インコーポレイテッド スブチリシン変異体
US7049485B2 (en) 2000-10-20 2006-05-23 Board Of Trustees Of Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
EP1493027B1 (en) 2002-03-01 2014-10-15 Codexis Mayflower Holdings, LLC Methods, systems, and software for identifying functional bio-molecules
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7620500B2 (en) 2002-03-09 2009-11-17 Maxygen, Inc. Optimization of crossover points for directed evolution
US20090183270A1 (en) * 2002-10-02 2009-07-16 Adams Thomas R Transgenic plants with enhanced agronomic traits
EP1654354A1 (en) 2003-08-11 2006-05-10 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
US7923552B2 (en) 2004-10-18 2011-04-12 SGF Holdings, LLC High yield method of producing pure rebaudioside A
US9386797B2 (en) 2011-02-17 2016-07-12 Purecircle Sdn Bhd Glucosyl stevia composition
EP2155869A2 (en) 2007-05-30 2010-02-24 Danisco US, INC., Genencor Division Variants of an alpha-amylase with improved production levels in fermentation processes
US8093028B2 (en) 2007-07-24 2012-01-10 Wisconsin Alumni Research Foundation Engineered glycosyltransferases with expanded substrate specificity
US8768871B2 (en) 2008-02-12 2014-07-01 Codexis, Inc. Method of generating an optimized, diverse population of variants
US8504498B2 (en) 2008-02-12 2013-08-06 Codexis, Inc. Method of generating an optimized, diverse population of variants
EP3023494B1 (en) 2008-06-13 2018-11-28 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
WO2009149577A1 (en) 2008-06-13 2009-12-17 Givaudan Sa Methods of identifying modulators of the bitter taste receptor tas2r44
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
ES2592377T3 (es) 2008-10-03 2016-11-29 Morita Kagaku Kogyo Co., Ltd. Nuevos glucósidos de esteviol
US8753861B2 (en) 2008-11-11 2014-06-17 Danisco Us Inc. Protease comprising one or more combinable mutations
US20150344512A1 (en) 2011-12-19 2015-12-03 Purecircle Usa Inc. Methods of purifying steviol glycosides and uses of the same
EP2544538B1 (en) 2010-03-12 2022-06-15 PureCircle USA Inc. High-purity steviol glycosides
US11155888B2 (en) 2010-03-12 2021-10-26 Purecircle Usa Inc. Stevia composition, production method and uses
EP2575432B1 (en) * 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
US9029426B2 (en) 2010-12-13 2015-05-12 Purecircle Sdn Bhd Highly soluble Rebaudioside D
US20140030381A1 (en) 2011-02-17 2014-01-30 Purecircle Usa Inc. Glucosyl stevia composition
US8733641B1 (en) 2011-06-14 2014-05-27 Digital Processing Systems, LLC. Electronic kiosk system and method for dispensing medical smart cards and managing healthcare information and services
EP2726651B1 (en) 2011-06-28 2018-11-07 Codexis, Inc. Protein variant generation by region shuffling
EP4124245A1 (en) 2011-12-19 2023-02-01 The Coca-Cola Company Beverage comprising rebaudioside x
MX355028B (es) 2012-01-23 2018-03-28 Dsm Ip Assets Bv Produccion de diterpeno.
US10000783B2 (en) 2012-03-16 2018-06-19 Suntory Holdings Limited Steviol glucosyltransferases and genes encoding the same
WO2013159055A1 (en) 2012-04-20 2013-10-24 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
MX395285B (es) 2012-05-22 2025-03-25 Purecircle Sdn Bhd Glucosidos de esteviol de alta pureza.
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
TW201402599A (zh) 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
MX2015003684A (es) 2012-09-25 2015-06-15 Cargill Inc Mezclas de esteviosidos.
EP2928321A1 (en) 2012-12-05 2015-10-14 Evolva SA Steviol glycoside compositions sensory properties
US20140171519A1 (en) 2012-12-19 2014-06-19 Indra Prakash Compositions and methods for improving rebaudioside x solubility
EP4414988A3 (en) 2013-01-31 2024-11-06 Codexis, Inc. Methods, systems, and software for identifying bio-molecules using models of multiplicative form
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
KR101404728B1 (ko) 2013-02-28 2014-06-09 씨제이제일제당 (주) 스테비오사이드로부터 리바우디오사이드 a를 제조하는 방법
US9717267B2 (en) 2013-03-14 2017-08-01 The Coca-Cola Company Beverages containing rare sugars
US20140272068A1 (en) 2013-03-14 2014-09-18 Indra Prakash Beverages containing rare sugars
WO2014153000A1 (en) 2013-03-14 2014-09-25 Chromocell Corporation Compounds, compositions, and methods for modulating sweet taste
US20140322389A1 (en) 2013-03-14 2014-10-30 Indra Prakash Beverages containing rare sugars
US20160029677A1 (en) 2013-03-15 2016-02-04 The Coca-Cola Company Novel glucosyl steviol glycosides, their compositions and their purification
EP2970354B1 (en) 2013-03-15 2018-05-30 The Coca-Cola Company Steviol glycosides and their compositions
US20140342044A1 (en) 2013-05-14 2014-11-20 Pepsico, Inc. Compositions and Comestibles
US20140342043A1 (en) 2013-05-14 2014-11-20 Pepsico, Inc. Rebaudioside Sweetener Compositions and Food Products Sweetened with Same
CA2912347A1 (en) 2013-05-31 2014-12-04 Dsm Ip Assets B.V. Extracellular diterpene production
US10039834B2 (en) 2013-07-12 2018-08-07 The Coca-Cola Company Compositions and methods using rebaudioside X to provide sweetness enhancement
US10905146B2 (en) 2013-07-12 2021-02-02 The Coca-Cola Company Compositions for improving rebaudioside M solubility
EP3021689B1 (en) 2013-07-15 2021-03-24 DSM IP Assets B.V. Diterpene production
EP3024941B1 (en) 2013-07-23 2019-07-03 DSM IP Assets B.V. Diterpene production in yarrowia
EP3536697A1 (en) 2013-07-31 2019-09-11 DSM IP Assets B.V. Recovery of steviol glycosides
CN103397064B (zh) 2013-08-14 2015-04-15 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
US20160235102A1 (en) 2013-09-23 2016-08-18 Almendra Americas, LLC Sweetener composition, sweetener products, and methods of sweetening
AU2014342939B2 (en) 2013-11-01 2018-03-22 Conagen Inc. Recombinant production of steviol glycosides
CN106471128A (zh) 2014-01-28 2017-03-01 百事可乐公司 一种酶法制备瑞鲍迪甙m的方法
US20150223505A1 (en) 2014-02-12 2015-08-13 Purecircle Usa Inc. Stevia composition, production method and uses
US10609942B2 (en) 2015-01-06 2020-04-07 James and Carol May Family, LLLP Compositions and methods for sweeteners
US10772346B2 (en) 2014-02-21 2020-09-15 James and Carol May Family, LLLP Compositions and methods for the solubilization of stevia glycosides
WO2015152707A1 (en) 2014-04-02 2015-10-08 Purecircle Sdn Bhd Compounds produced from stevia and process for producing the same
US20180020709A1 (en) 2014-04-16 2018-01-25 Purecircle Usa Inc. Rebaudioside m biosynthetic production and recovery methods
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
US10264811B2 (en) 2014-05-19 2019-04-23 Epc Natural Products Co., Ltd. Stevia sweetener with improved solubility
GB2526383B (en) 2014-05-20 2018-01-31 Tate & Lyle Ingredients Americas Llc Improved sweetener
US10357052B2 (en) 2014-06-16 2019-07-23 Sweet Green Fields USA LLC Rebaudioside A and stevioside with improved solubilities
US10485256B2 (en) 2014-06-20 2019-11-26 Sweet Green Fields International Co., Limited Stevia sweetener with improved solubility with a cyclodextrin
CN104151378A (zh) 2014-08-12 2014-11-19 济南汉定生物工程有限公司 一种甜菊糖甙rm的提纯方法
EP3683315A1 (en) 2014-08-19 2020-07-22 Purecircle SDN BHD Method for preparing rebaudioside m
WO2016036980A1 (en) 2014-09-03 2016-03-10 Chromocell Corporation Compounds, compositions, and methods for modulating sweet taste
IL234525B (en) 2014-09-08 2018-05-31 Unavoo Food Tech Ltd The composition containing a filler and a flavoring agent and its use
CA2960693A1 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
ES2903031T3 (es) 2014-09-11 2022-03-30 Pepsico Inc Potenciador de dulzor
EP4166010A1 (en) 2014-09-19 2023-04-19 PureCircle SDN BHD High-purity steviol glycosides
KR101765369B1 (ko) 2014-09-19 2017-08-08 한국과학기술원 돌외 유래의 신규한 당전이효소 및 이의 용도
BR112017006163B1 (pt) 2014-10-03 2022-04-05 Conagen Inc Produto de consumo oral e método para adoçar o mesmo
EP3204506B1 (en) 2014-10-08 2022-01-12 DSM IP Assets B.V. Steviol glycoside production
CN107109453A (zh) 2014-11-05 2017-08-29 马努斯生物合成股份有限公司 甜菊醇糖苷的微生物产生
US20160165941A1 (en) 2014-11-21 2016-06-16 Flipn'Sweet, LLC Sugar substitute compositions comprising digestion resistent soluble fiber
US20170258121A1 (en) 2014-11-24 2017-09-14 Cargill, Incorporated Glycoside compositions
CN107249356B (zh) 2014-12-17 2021-08-06 嘉吉公司 用于口服摄入或使用的甜菊醇糖苷化合物、组合物以及用于增强甜菊醇糖苷溶解度的方法
EP3245196A4 (en) 2015-01-13 2018-06-13 Chromocell Corporation Compounds, compositions, and methods for modulating sweet taste
EP3250686A1 (en) 2015-01-30 2017-12-06 Evolva SA Production of steviol glycosides in recombinant hosts
CA3220591A1 (en) 2015-03-11 2016-09-15 Morita Kagaku Kogyo Co., Ltd. Sweetner composition and food containing same
WO2016146711A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
CA2979957A1 (en) 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
KR102569053B1 (ko) 2015-04-14 2023-08-21 코나겐 인크. 조작된 전체 세포 촉매를 사용한 비칼로리 감미료의 제조
CA2985669C (en) 2015-05-20 2022-05-03 Cargill, Incorporated Glycoside compositions
US10612065B2 (en) 2015-05-29 2020-04-07 Cargill, Incorporated Heat treatment to produce glycosides
EP3303552A4 (en) 2015-05-29 2019-01-16 Cargill, Incorporated FERMENTATION METHOD FOR THE PRODUCTION OF STEVIOL GLYCOSIDES WITH MULTIPHASE SUPPLY
EP3303553B1 (en) 2015-05-29 2022-07-27 Cargill, Incorporated Fermentation methods for producing steviol glycosides using high ph
CN105200098A (zh) 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法
US10517321B2 (en) 2015-07-10 2019-12-31 Sweet Green Fields USA LLC Compositions of steviol multiglycosylated derivatives and stevia components
CN107820394A (zh) 2015-07-10 2018-03-20 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷组合物
US11124535B2 (en) 2015-07-10 2021-09-21 Dsm Ip Assets B.V. Steviol glycoside composition
US10844414B2 (en) 2015-08-06 2020-11-24 Cargill, Incorporated Methods for producing steviol glycosides in engineered yeast
US20190203244A1 (en) 2015-08-20 2019-07-04 Pepsico, Inc. Preparation of rebaudioside m in a single reaction vessel
WO2017053980A1 (en) 2015-09-25 2017-03-30 The Coca-Cola Company Steviol glycoside blends, compositions and methods
EP4190172B1 (en) 2015-10-02 2025-11-26 The Coca-Cola Company Sweetener composition comprising steviol glycoside sweeteners with improved flavor profiles
US20170105432A1 (en) 2015-10-16 2017-04-20 Senomyx, Inc. Sweetener and flavor enhancer formulations
WO2017068017A1 (en) 2015-10-23 2017-04-27 Dsm Ip Assets B.V. Low sugar flavoured yogurt
US11647771B2 (en) 2015-10-26 2023-05-16 Purecircle Usa Inc. Steviol glycoside compositions
WO2017093895A1 (en) 2015-11-30 2017-06-08 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
EP3387136B1 (en) 2015-12-10 2022-01-05 Evolva SA Production of steviol glycosides in recombinant hosts
PL3393258T3 (pl) 2015-12-24 2026-02-23 Danone Us, Llc Sposób wytwarzania odcedzanego fermentowanego produktu mlecznego z zastosowaniem laktazy
CN108777992A (zh) 2016-01-07 2018-11-09 谱赛科美国股份有限公司 高溶解性甜菊醇糖苷
US20190021382A1 (en) 2016-03-01 2019-01-24 Wm. Wrigley Jr. Company Long-lasting sweetener formulations
US20170258120A1 (en) 2016-03-09 2017-09-14 Merisant US, Inc. Liquid sweetener compositions
WO2017156432A1 (en) 2016-03-10 2017-09-14 Chromocell Corporation Steviol glycoside esters
US11149050B2 (en) 2016-03-14 2021-10-19 Purecircle Usa Inc. Highly soluble steviol glycosides
US20190116835A1 (en) 2016-03-28 2019-04-25 The Coca-Cola Company Sweetness and Taste Improvement of Steviol Glycoside or Mogroside Sweeteners with Flavonids
ES2980286T3 (es) 2016-03-31 2024-09-30 Suntory Holdings Ltd Bebida que contiene estevia
CN109152393A (zh) 2016-03-31 2019-01-04 三得利控股株式会社 含有甜菊的饮料
AU2017246876B2 (en) 2016-04-06 2021-09-23 The Coca-Cola Company Sweetness and taste improvement of steviol glycoside or mogroside sweeteners
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017189778A1 (en) 2016-04-26 2017-11-02 Chromocell Corporation Methods, compounds, and compositions, for modulating sweet taste
US20190124953A1 (en) 2016-04-29 2019-05-02 Pepsico, Inc. Novel steviol glycosides blends
US11985995B2 (en) 2016-05-10 2024-05-21 The Coca-Cola Company Methods of freeze drying compositions containing rebaudioside M and rebaudioside D
WO2017207484A1 (en) * 2016-05-31 2017-12-07 Universiteit Gent Mutant sucrose synthases and their uses
US20170352267A1 (en) 2016-06-02 2017-12-07 GM Global Technology Operations LLC Systems for providing proactive infotainment at autonomous-driving vehicles
BR112018076109B1 (pt) 2016-06-14 2022-11-01 Purecircle Usa Inc Processo para produzir uma composição de glicosídeos de esteviol, composição adoçante, composição de paladar, ingrediente alimentar e alimento, bebida, produto cosmético e farmacêutico
US20190127772A1 (en) 2016-06-15 2019-05-02 Codexis, Inc. Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate
CN108135238A (zh) 2016-08-04 2018-06-08 百事可乐公司 甜味剂组合物
CA3033243A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
WO2018029272A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
PT3497222T (pt) 2016-08-12 2022-02-09 Amyris Inc Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos
US10085472B2 (en) 2016-08-29 2018-10-02 Pepsico, Inc. Compositions comprising rebaudioside J
KR102905512B1 (ko) 2017-02-03 2025-12-29 테이트 앤드 라일 솔루션스 유에스에이 엘엘씨 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
CN106866757B (zh) 2017-03-16 2020-06-26 诸城市浩天药业有限公司 甜菊糖m苷晶型及制备方法和用途
US20170354175A1 (en) 2017-05-15 2017-12-14 Senomyx, Inc. Sweetener composition
IL280302B2 (en) 2018-07-30 2025-09-01 Codexis Inc Engineered glycosyltransferases and methods for glycosylation of steviol glycoside

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
JP2016506739A (ja) 2013-02-06 2016-03-07 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． レバウディオサイドｄおよびレバウディオサイドｍの改良された産生方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NCBI Reference Sequence: XP_006367681.1 (2016.01.05.) 1부.*

Also Published As

Publication number Publication date
AU2020289868A1 (en) 2021-01-28
IL304833A (en) 2023-09-01
WO2018144675A1 (en) 2018-08-09
TW201837171A (zh) 2018-10-16
MY199186A (en) 2023-10-19
TW202237833A (zh) 2022-10-01
JP7176786B2 (ja) 2022-11-22
JP2022163224A (ja) 2022-10-25
US12404499B2 (en) 2025-09-02
TWI797103B (zh) 2023-04-01
US20180223264A1 (en) 2018-08-09
AU2022268390A1 (en) 2022-12-15
WO2018144679A2 (en) 2018-08-09
JP7248322B2 (ja) 2023-03-29
KR20230162160A (ko) 2023-11-28
CN118956802A (zh) 2024-11-15
CN110914445B (zh) 2024-08-27
AU2022268390B2 (en) 2024-11-28
JP2021036911A (ja) 2021-03-11
EP3577229A4 (en) 2020-12-23
AU2018215335A1 (en) 2019-08-01
JP7248302B2 (ja) 2023-03-29
WO2018144679A3 (en) 2018-12-13
CA3175100A1 (en) 2018-08-09
KR20260006703A (ko) 2026-01-13
US20210054349A1 (en) 2021-02-25
AU2018215335B2 (en) 2020-10-01
IL268121B2 (en) 2024-01-01
AU2020289868B2 (en) 2022-12-01
MX391104B (es) 2025-03-21
IL268121A (en) 2019-09-26
IL304833B2 (en) 2025-11-01
US11920167B2 (en) 2024-03-05
PH12019501698A1 (en) 2019-11-04
US20260002136A1 (en) 2026-01-01
US20220306998A1 (en) 2022-09-29
EP3878967A3 (en) 2021-12-08
MX2022003824A (es) 2022-05-25
EP3577229A2 (en) 2019-12-11
KR102606350B1 (ko) 2023-11-23
EP3882354A2 (en) 2021-09-22
JP2021087447A (ja) 2021-06-10
CN110914445A (zh) 2020-03-24
SG11201906480QA (en) 2019-08-27
MX2021005862A (es) 2021-08-11
EP3878967A2 (en) 2021-09-15
IL304833B1 (en) 2025-07-01
AU2020289865A1 (en) 2021-01-28
IL321402A (en) 2025-08-01
JP2020506704A (ja) 2020-03-05
AU2020289865B2 (en) 2022-12-01
CA3050310A1 (en) 2018-08-09
KR20190124723A (ko) 2019-11-05
IL268121B1 (en) 2023-09-01
MX2019009233A (es) 2019-09-19
TW202237834A (zh) 2022-10-01
US12291728B2 (en) 2025-05-06
CA3175070A1 (en) 2018-08-09
BR112019015992A2 (pt) 2020-05-26
EP3882354A3 (en) 2021-12-15
MX2021005861A (es) 2021-08-11
US20210054348A1 (en) 2021-02-25

Similar Documents

Publication Publication Date Title
KR102905512B1 (ko) 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
JP7584474B2 (ja) 操作されたグリコシルトランスフェラーゼおよびステビオールグリコシドのグルコシル化方法
BR122024013399A2 (pt) Sacarose sintase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, método para produzir pelo menos uma variante de sacarose sintase engenheirada, composição, e, método
BR122024013384A2 (pt) Glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, método para produzir pelo menos uma glicosiltransferase engenheirada, composição, e, método
BR122024013406A2 (pt) Sacarose sintase engenheirada, glicosiltransferase engenheirada, polinucleotídeo engenheirado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase engenheirada, e para produzir pelo menos uma variante de sacarose sintase engenheirada, composição, e, métodos

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

St.27 status event code: A-0-1-A10-A18-div-PA0104

St.27 status event code: A-0-1-A10-A16-div-PA0104

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

D22 Grant of ip right intended

Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D22-EXM-PE0701 (AS PROVIDED BY THE NATIONAL OFFICE)

PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

A16 Divisional, continuation or continuation in part application filed

Free format text: ST27 STATUS EVENT CODE: A-0-1-A10-A16-DIV-PA0104 (AS PROVIDED BY THE NATIONAL OFFICE)

A18 Application divided or continuation or continuation in part accepted

Free format text: ST27 STATUS EVENT CODE: A-0-1-A10-A18-DIV-PA0104 (AS PROVIDED BY THE NATIONAL OFFICE)

PA0104 Divisional application for international application

St.27 status event code: A-0-1-A10-A18-div-PA0104

St.27 status event code: A-0-1-A10-A16-div-PA0104

F11 Ip right granted following substantive examination

Free format text: ST27 STATUS EVENT CODE: A-2-4-F10-F11-EXM-PR0701 (AS PROVIDED BY THE NATIONAL OFFICE)

PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

U12 Designation fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U12-OTH-PR1002 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

Q13 Ip right document published

Free format text: ST27 STATUS EVENT CODE: A-4-4-Q10-Q13-NAP-PG1601 (AS PROVIDED BY THE NATIONAL OFFICE)