KR102780317B1 - Compositions for inhibiting sebum secretion comprising Sargassum fusiforme extracts - Google Patents

Abstract

The present invention relates to a composition for controlling sebum secretion, which contains a sesame extract as an effective ingredient. The sesame extract of the present invention not only effectively controls sebum secretion by inhibiting differentiation of sebum cells, but also has excellent safety for the human body as it does not irritate the skin, and thus can be usefully used in cosmetics for controlling sebum secretion, or in medicines, external skin preparations, and foods for preventing, treating, or improving diseases caused by abnormal sebum secretion.

Description

Compositions for inhibiting sebum secretion comprising Sargassum fusiforme extracts as an effective ingredient

The present invention relates to a composition for suppressing sebum secretion comprising a sesame extract as an effective ingredient, and more specifically, to a composition comprising a sesame extract as an effective ingredient, which can effectively control sebum secretion by suppressing differentiation of sebum cells.

Sebum is a type of lipid that is produced by sebaceous cells located in the sebaceous glands connected to the isthmus of the hair follicle and secreted through pores onto the skin surface. Sebum secreted in moderation forms a lipid film on the surface of the skin and hair, adding flexibility, elasticity, and gloss to the skin and hair, and performs a sterilizing action while maintaining moisture balance.

Sebaceous glands exist in high density on the face, and sebaceous cells migrate from the outermost layer of the sebaceous gland, the peripheral zone (PZ), to the inner layer, the maturation zone (MZ), where they differentiate, and when they finally reach the innermost layer, the necrotic zone (NZ), the nucleus and membrane are lost, and sebum is secreted within the cell.

It is known that sebum secretion is involved in excessive secretion of androgen, a sex hormone, PPARγ (peroxidome proliferator activated receptor γ), Wnt (Wingless) signaling, insulin, etc., which regulate retinoid and lipogenic gene expression, and is ultimately regulated by lipid synthesis or sebum cell differentiation mechanisms. Various fatty acids also affect sebum production, and research results show that palmitic acid, a saturated fatty acid, promotes lipid synthesis in sebum cells and causes an inflammatory response.

Sebum is composed of neutral fat, squalene, cholesterol, wax, etc., and neutral fat in particular is known to be broken down into free fatty acids by bacterial lipase, causing an inflammatory reaction. This inflammatory reaction, along with excessive sebum production, causes or worsens seborrheic dermatitis or scalp dermatitis.

Seborrheic dermatitis is an eczema caused by excessive sebum secretion, Malassezia infection, and abnormal proliferation of the epidermis. It mainly occurs in areas with active sebaceous glands, such as the scalp or face. Seborrheic dermatitis that appears on the scalp is called seborrheic dermatitis and is known to worsen hair loss. This is because the inflammatory response aggravated by seborrheic dermatitis induces itching and damages hair follicles and hair. Therefore, attempts are being made to alleviate skin diseases such as seborrheic dermatitis or scalp dermatitis by controlling sebum secretion or suppressing excessive sebum production.

Sargassum fusiforme is a seaweed belonging to the brown algae class, Sargassum family, and Sargassum genus, and is mainly used for food in Korea, Japan, and China. It lives in colonies in the intertidal zone, and was also eaten as relief during famines and food shortages. This is because Sargassum contains a large amount of nutrients among seaweeds, and is especially rich in minerals such as iron, calcium, and iodine, so it is also used as a health food to supplement minerals for growing children. In addition, it is effective in preventing hair loss with its rich dietary fiber and vitamins, and fucoidan enhances immunity, and alginic acid is excellent at excreting heavy metals and fine dust from the body, so it is popular as an anticancer food.

Regarding the efficacy of the above-mentioned tilia, Korean Patent No. 10-1694945 discloses a composition for sunscreen containing a tilia extract as an active ingredient, and Korean Patent No. 10-1418916 discloses a pharmaceutical composition for treating atopic disease using an ethyl acetate extract fractionated from an ethyl acetate extract obtained by extracting an ethanol extract of tilia with n-hexane and extracting the remaining residue with ethyl acetate. In addition, Korean Patent Publication No. 10-2021-0106661 discloses a cosmetic composition for skin whitening or wrinkle improvement containing a hot water extract of tilia as an active ingredient. However, the sebum control effect of the tilia extract has not yet been revealed.

Accordingly, the inventors of the present invention conducted research to find a natural material capable of controlling sebum production by inhibiting the differentiation of sebum cells, and as a result, discovered that a seaweed extract can effectively control sebum production, thereby completing the present invention.

One object of the present invention is to provide a cosmetic composition for suppressing sebum secretion containing a turmeric extract as an effective ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema, comprising a turmeric extract as an effective ingredient.

Another object of the present invention is to provide a composition for external application of skin for the prevention, improvement or treatment of seborrheic dermatitis, seborrheic dermatitis, folliculitis or seborrheic eczema, containing a turmeric extract as an effective ingredient.

Another object of the present invention is to provide a food composition for suppressing sebum secretion containing a sesame extract as an effective ingredient.

Another object of the present invention is to provide a method for regulating sebum secretion in a subject and preventing, improving, or treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema by administering to the subject a composition for inhibiting sebum secretion containing a turmeric extract as an effective ingredient.

In one embodiment, the present invention provides a composition for inhibiting sebum secretion comprising a sesame extract as an effective ingredient.

In the present invention, " Sargassum fusiforme " is an edible seaweed belonging to the family Sargassum, a brown algae, and is a perennial seaweed that lives in the lower intertidal zone of Korea, Japan, and China.

In one specific embodiment, the thuja extract of the present invention is characterized in that it is produced by a method including the steps of (S10) extracting thuja with hot water; (S20) immersing the thuja hot water extract in an organic solvent; and (S30) obtaining only a precipitate from the immersion material.

Hereinafter, the method for producing the thuja extract of the present invention will be specifically described.

(S10) Step is the step of hot water extraction.

More specifically, it is a step of immersing the thuja powder in water and heating it to 90 to 120℃, preferably 100℃, to extract it. At this time, the extraction time is preferably 10 minutes to 4 hours, more preferably 60 minutes to 3 hours, and most preferably 2 hours, but is not limited thereto. In addition, the number of extraction times is preferably 1 to 5 times, more preferably 1 to 3 times of repeated extraction, and most preferably 2 times, but is not limited thereto.

Step (S20) is a step of immersing the hot water extract of the thuja prepared in step (S10) in an organic solvent.

The above organic solvent may be a lower alcohol having 1 to 4 carbon atoms (such as ethanol, methanol, propanol or butanol), preferably ethanol, more preferably a 60 to 80% ethanol solution, even more preferably a 65 to 75% ethanol solution, and most preferably a 70% ethanol solution.

The above immersion can be carried out for a period of time during which the useful components of the seaweed are sufficiently extracted by the organic solvent, preferably 60 to 180 minutes, more preferably 90 to 150 minutes, and even more preferably 120 minutes. In addition, the above immersion is preferably carried out at room temperature.

Meanwhile, the hot water extract of tangle immersed in the organic solvent can be immersed in a solution state, but it is preferable to dry the hot water extract of tangle and immerse it in a powdered state so that the useful components of tangle can be quickly extracted. Here, the drying can be performed by a method such as freeze drying, reduced pressure drying, vacuum drying, boiling drying, or spray drying, but is not necessarily limited thereto.

Step (S30) is a step of obtaining only a sediment from the immersion material of step (S20).

The above-mentioned precipitate can be obtained by a method such as centrifugation, sedimentation, etc., separating the supernatant and the precipitate, removing the supernatant, and obtaining only the precipitate.

The above sediment can be used as is, but can also be used in the form of a diluted solution, concentrate, purified product, dried product, etc.

In the present invention, "sebum" is a secretion from the sebaceous glands in the skin, and plays a role in maintaining moisture in the skin including the scalp and face and preventing the invasion of microorganisms. In particular, in the case of women in their 20s and older, excessive secretion of sebum causes many cosmetic problems such as skin shine, makeup lifting, and enlarged pores. In addition, excessive secretion of sebum is known to have a great effect on seborrheic dermatitis, seborrheic scalp dermatitis, folliculitis, or seborrheic eczema.

In one specific embodiment, it was confirmed that the seaweed extract according to the present invention has an excellent effect of inhibiting the formation of lipid droplets in sebocytes (human sebocytes, Cellprogen), thereby having an effect of inhibiting sebum secretion.

In another specific embodiment, it was confirmed that the thuja extract according to the present invention does not irritate human skin and has excellent safety.

Therefore, the composition according to the present invention regulates or suppresses sebum secretion of the skin, and has high safety for the skin, so it can be usefully used for suppressing and improving sebum secretion, or preventing and treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema caused by abnormal sebum secretion, and can be usefully used in cosmetics, medicines, and foods for this purpose.

In this specification, the term "sebum secretion control", "sebum secretion suppression", or "sebum secretion improvement" refers to reducing the sebum secretion amount to be the same as or similar to the normal case when the sebum secretion amount is excessive compared to the normal case. Here, excessive refers to an increase of 5% or more, 6% or more, 7% or more, 8% or more, 9% or more, 10% or more, etc. compared to the normal case. In addition, an increase of 0% or 0.1% or more to less than 5% compared to the normal case when the sebum secretion amount is the same as or similar to the normal case.

In this specification, the term "abnormal sebum secretion" has the same meaning as the term "excessive case" mentioned above, and refers to an increase in sebum secretion of 5% or more, 6% or more, 7% or more, 8% or more, 9% or more, 10% or more, etc., compared to normal cases.

As used herein, the terms "prevention" or "suppression" mean reducing the occurrence of a disease or condition in a subject who has not been diagnosed as having a disease or condition that may be caused by abnormal sebum secretion, but is predisposed to such a disease or condition.

In this specification, the term "treatment" or "improvement" means (a) inhibition of the development of a disease or condition that may occur due to abnormal secretion of sebum in a subject, (b) alleviation of a disease or condition that may occur due to abnormal secretion of sebum, and (c) elimination of a disease or condition that may occur due to abnormal secretion of sebum.

As used herein, the term “subject” refers to a mammal, including a human, monkey, cow, horse, pig, sheep, dog, cat, rat, mouse, chimpanzee, etc., having a disease whose symptoms can be improved by administering the composition of the present invention.

In the composition of the present invention, the effective ingredient of the tilia extract may be included in an amount of 0.0001 to 10.0 wt%, preferably 0.001 to 10 wt%, based on the total weight of the composition. If the content of the tilia extract is less than 0.0001 wt%, the effect of inhibiting and improving sebum secretion is too weak, and if it exceeds 10.0 wt%, there is a problem that the increase in effect due to the increase in content is insignificant and the stability of the formulation is not secured.

As one specific embodiment, the present invention provides a cosmetic composition for suppressing sebum secretion, comprising a composition containing the above-mentioned extract of the plant as an effective ingredient.

The above-mentioned effects of the extract of the plant and its effect of regulating sebum secretion and the prevention and improvement effects of seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema are as described above.

The cosmetic composition of the present invention can be generally applied to areas with a lot of sebum secretion, such as the face, scalp, or back, and is not limited to these areas, but can be applied to any area with developed sebaceous glands.

The cosmetic composition of the present invention contains, in addition to the effective ingredient, the thuja extract, ingredients commonly used in cosmetic compositions, and includes conventional auxiliary agents and carriers such as antioxidants, solubilizers, stabilizers, vitamins, pigments, and fragrances.

The cosmetic composition of the present invention can be manufactured into any formulation generally manufactured in the art, and for example, can be formulated into a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. In addition, it can be manufactured into the formulation of a nourishing cream, astringent toner, flexible toner, lotion, essence, shampoo, nourishing gel, or massage cream.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as a carrier component.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizer or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as carrier components.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.

In particular, the cosmetic composition of the present invention provides a hair care cosmetic composition that suppresses sebum secretion of the scalp by applying it to the scalp or hair.

The above-mentioned cosmetic compositions for hair care include, for example, hair shampoo, hair rinse, hair conditioner, hair treatment, hair tonic, scalp treatment, hair lotion, hair cream, hair mist, hair ampoule, hair pack, hair essence, hair oil, scalp scaler, hair nourishing toner, and hair dye.

The cosmetic composition of the present invention may contain 0.0001 to 10.0 wt%, preferably 0.001 to 10 wt%, of the tilia extract as an active ingredient based on the weight of the total composition. If the content of the tilia extract is less than 0.0001 wt%, the effect of suppressing and improving sebum secretion is too weak, and if it exceeds 10.0 wt%, there is a problem that the increase in effect due to the increase in content is insignificant and the stability of the formulation is not secured.

In another specific embodiment, the present invention provides a pharmaceutical composition for preventing or treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema, comprising a composition containing the extract of the present invention as an effective ingredient.

The preventive and therapeutic effects of the above-mentioned extract of the plant and its sebum secretion regulating effect on seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema are as described above.

The term "pharmaceutical composition" of the present invention means a composition manufactured for the purpose of preventing or treating a disease, and each may be formulated and used in various forms according to conventional methods. For example, it may be formulated as an oral formulation such as a powder, granule, tablet, capsule, suspension, emulsion, syrup, or aerosol, and may be formulated and used as a parenteral formulation using a diluent or excipient such as a lubricant, a humectant, a flavoring agent, an emulsifier, a suspending agent, a preservative, or a surfactant. In addition, it may be formulated and used in the form of an external preparation, a suppository, an external skin preparation, and a sterile injection solution. As a specific example, the composition of the present invention may be used in an oral dosage form by oral administration. As another specific example, the composition of the present invention may be used by a method such as directly applying or spraying it onto hair and skin with excessive sebum secretion.

In addition, depending on each formulation, it can be manufactured by additionally including pharmaceutically acceptable carriers, such as buffers, analgesics, solubilizers, isotonic agents, stabilizers, and carriers known in the art.

The term "pharmaceutically acceptable carrier" used in the present invention may mean a carrier or diluent that does not stimulate a living organism and does not inhibit the biological activity and properties of the active ingredient to be injected. The type of the carrier usable in the present invention is not particularly limited, and any carrier that is commonly used in the relevant technical field and is pharmaceutically acceptable may be used. Non-limiting examples of the carrier include saline solution, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and the like. These may be used alone or in combination of two or more. The carrier may be a non-naturally occurring carrier.

The composition of the present invention can be administered in a pharmaceutically effective amount. The pharmaceutically effective amount means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not causing side effects, and the effective dosage level can be determined according to factors including the patient's health condition, the type and severity of the disease, the activity of the drug, the sensitivity to the drug, the administration method, the administration time, the administration route and the excretion rate, the treatment period, the drugs used in combination or simultaneously, and other factors well known in the medical field. Specifically, the dosage is generally 0.01 mg to 5000 mg per 1 kg of the body weight of the administered subject per day, and may be administered once or several times a day at regular intervals depending on the judgment of a doctor or pharmacist, but is not limited thereto.

The pharmaceutical composition of the present invention may contain a tilia extract as an active ingredient in an amount of 0.0001 to 10.0 wt%, preferably 0.001 to 10 wt%, based on the weight of the total composition. If the content of the tilia extract is less than 0.0001 wt%, the effect of suppressing and improving sebum secretion is insignificant, and thus the effect of preventing and treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema cannot be obtained. In addition, if it exceeds 10.0 wt%, there is a problem that the increase in effect due to the increase in content is insignificant and the stability of the formulation is not secured.

In addition, the pharmaceutical composition of the present invention can be used alone or in combination with or as an appropriate set of other pharmaceutically active compounds that exhibit a preventive or therapeutic effect on seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema.

The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And may be administered singly or in multiple doses. It is important to administer an amount that can achieve the maximum effect with the minimum amount without causing side effects by considering all of the above factors, and this can be easily determined by those skilled in the art.

In the present invention, the term "administration" means introducing the pharmaceutical composition of the present invention into a subject by any appropriate method, and the route of administration of the composition of the present invention may be through various routes, such as oral or parenteral, as long as it can reach the target tissue.

The method of administration of the pharmaceutical composition according to the present invention is not particularly limited, and may follow a method commonly used in the relevant technical field. As a non-limiting example of the above-mentioned method of administration, the composition may be administered orally or parenterally. Specifically, the composition of the present invention may be used by a method such as directly applying or spraying it on areas such as hair or skin where sebum is secreted excessively.

The pharmaceutical composition according to the present invention can be prepared in various dosage forms depending on the intended administration method.

The frequency of administration of the composition of the present invention is not particularly limited, but may be administered once a day or administered several times in divided doses.

In another specific embodiment, the present invention provides a composition for external application of skin for the prevention, improvement or treatment of seborrheic dermatitis, seborrheic dermatitis, folliculitis or seborrheic eczema, comprising a composition containing the extract of the present invention as an effective ingredient.

The preventive and therapeutic effects of the above-mentioned extract of the plant and its sebum secretion regulating effect on seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema are as described above.

The composition for external application of the skin according to the present invention can be formulated containing a cosmetically or dermatologically acceptable medium or base. It can be provided in any formulation suitable for topical application, for example, in the form of a solution, a gel, a solid, a pasty anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule, or a vesicular dispersion of ionic (liposomes) and nonionic types, or in the form of a cream, a skin, a lotion, a powder, an ointment, a spray, a pack or a concealer stick. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions can be prepared according to conventional methods in the art.

In addition, the skin external composition according to the present invention may contain adjuvants commonly used in the fields of cosmetology or dermatology, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestering agents, chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics. The adjuvants are introduced in an amount commonly used in the fields of cosmetology or dermatology.

The thuja extract included in the above composition is not particularly limited thereto, but may be contained in an amount of 0.0001 to 10.0 wt%, more preferably 0.001 to 10 wt%, based on the total weight of the composition. If the content of the thuja extract is less than 0.0001 wt%, the effect of suppressing and improving sebum secretion is insignificant, and thus the effect of preventing and treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema cannot be obtained. In addition, if it exceeds 10.0 wt%, there is a problem that the increase in effect due to the increase in content is insignificant and the stability of the formulation is not secured.

In another specific embodiment, the present invention provides a food composition for controlling sebum secretion, comprising a composition containing the extract of the present invention as an effective ingredient.

The above-mentioned effects of the extract of the plant and its effect of regulating sebum secretion and the prevention and improvement effects of seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema are as described above.

When the composition of the present invention is used as a food additive, the thuja extract can be added as it is or used together with other foods or food ingredients, and can be used appropriately according to a conventional method. The mixing amount of the effective ingredient can be appropriately determined according to the purpose of use (prevention, health, or therapeutic treatment), and a food-related acceptable food auxiliary additive can be additionally included. The thuja extract of the present invention has been used for food, and its safety has been confirmed through human safety tests, so there is no significant limitation on the mixing amount.

The food composition of the present invention can include all foods in the conventional sense, and can be used interchangeably with terms known in the art, such as functional food and health functional food.

There is no limitation on the type of food in which the food composition of the present invention can be used. In addition, a composition containing the extract of the present invention as an active ingredient can be prepared by mixing other appropriate auxiliary ingredients that can be contained in foods and known additives according to the selection of a person skilled in the art. Examples of foods to which it can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and it can be prepared by adding it to juice, tea, jelly, and juice prepared using the extract according to the present invention as a main ingredient.

In addition, foods applicable to the present invention may include all foods, such as special nutritional foods (e.g., formulated milk, infant/toddler food, etc.), processed meat products, fish products, tofu, starch jelly, noodles (e.g., ramen, noodles, etc.), health supplements, seasoned foods (e.g., soy sauce, soybean paste, red pepper paste, mixed sauce, etc.), sauces, confectionery (e.g., snacks), processed dairy products (e.g., fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi types, pickled vegetables, etc.), beverages (e.g., fruit and vegetable beverages, soy milk, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.).

The thuja extract included in the above composition is not particularly limited thereto, but is contained in an amount of 0.0001 to 10 wt%, more preferably 0.001 to 10 wt%, based on the total weight of the composition. If the content of the thuja extract is less than 0.0001 wt%, the effect of suppressing and improving sebum secretion is insignificant, and thus the effect of preventing and treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema cannot be obtained. In addition, if it exceeds 10.0 wt%, there is a problem that the increase in effect due to the increase in content is insignificant and the stability of the formulation is not secured.

When the health functional food composition of the present invention is used in the form of a beverage, it may contain various sweeteners, flavoring agents, or natural carbohydrates as additional ingredients, like conventional beverages. In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, it may contain fruit pulp for the production of natural fruit juice, fruit juice drinks, and vegetable drinks.

In another aspect, the present invention provides a method for regulating sebum secretion in a subject and preventing, improving, or treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema by administering to the subject a composition for inhibiting sebum secretion containing the above-described extract of the present invention as an effective ingredient.

The above-mentioned effects of the extract of the plant and its effect of regulating sebum secretion and the prevention and improvement effects of seborrheic dermatitis, seborrheic dermatitis, folliculitis, and seborrheic eczema are as described above.

The above-described preventive or therapeutic method of the present invention may specifically include a step of administering the composition in a pharmaceutically effective amount to a subject who has developed or is likely to develop seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema due to sebum secretion abnormality. The method of administration is as described above.

The above prevention may mean any act of inhibiting or delaying the onset of seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema by administering to a subject a composition for controlling sebum secretion according to the present invention.

The above treatment may mean any act of improving or benefiting the symptoms and symptoms of a disease caused by abnormal sebum secretion by administering a composition for controlling sebum secretion according to the present invention to a subject suspected of having symptoms and diseases caused by abnormal sebum secretion.

The pharmaceutical composition containing the above-mentioned extract of the plant is as described above.

The composition of the present invention may contain, for administration, a pharmaceutically acceptable carrier, excipient or diluent in addition to the effective ingredients described above. The carrier, excipient and diluent may include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The appropriate dosage of the composition of the present invention varies depending on the patient's condition and weight, the degree of the disease, the drug form, and the time, but can be appropriately selected by a person skilled in the art. Specifically, the dosage of the above-mentioned thuja extract can be 1 to 500 mg/kg.

Meanwhile, the effective ingredient of the present invention, the thuja extract, has not only been used for food, but has also been found to be harmless to the human body as a result of a skin cumulative irritation test. Therefore, the thuja extract of the present invention has almost no toxicity or side effects, so it can be used safely even for long-term use, and can be safely used in cosmetics, medicines, foods, and external skin preparations.

The composition for controlling sebum secretion comprising the extract of the present invention as an effective ingredient is free of skin irritation and cytotoxicity, has excellent human safety, and is effective in suppressing sebum secretion by sebum cells, so it can be safely used as an effective ingredient in a cosmetic or pharmaceutical composition for controlling sebum secretion.

Figure 1 is a drawing showing the effect of a sesame extract on skin secretion inhibition through inhibition of sebum cell differentiation according to one embodiment of the present invention.

Hereinafter, in order to help understand the present invention, examples and the like will be described in detail. However, the examples according to the present invention may be modified in various different forms, and the scope of the present invention should not be construed as being limited to the following examples. The examples of the present invention are provided to more completely explain the present invention to a person having average knowledge in the art.

Example 1: Preparation of a turmeric extract

Jeju seaweed was collected, washed to completely remove foreign substances and impurities, dried in the shade at 20 to 35°C, and extracted with 100°C water for 2 hours. The extract was freeze-dried and then precipitated in a 70% ethanol solution for 2 hours. After precipitation, only the precipitate, excluding the supernatant, was recovered, and this was dried to manufacture a seaweed extract into a powder.

Example 2: in vitro Measurement of the sebum secretion inhibition effect of the extract from the

To determine whether the extract of the plant has any effect on sebum secretion, the inhibitory ability of the extract of the plant to inhibit the formation of lipid droplets in sebum cells was measured. Specifically, human sebocytes (Celprogen) were cultured in complete media containing serum, and then seeded on a 24-well plate coated with collagen (1 × 10 ⁴ cells/well) and cultured in a CO ₂ incubator at 37°C for 24 hours. The extract of the plant of Example 1 or 50 μM of EGCG was dissolved together with palmitic acid in DMEM medium containing 1% FBS, treated to sebum cells, and cultured for 48 hours. For lipid droplet staining, the cell culture supernatant was removed, washed with PBS, and then treated with 4% formaldehyde for 10 minutes. After washing again with PBS, 10 μg/ml Nile Red was mixed with PBS at a ratio of 1:100 and treated, and left at room temperature for 15 minutes. After washing with PBS, the nucleus was stained with Dapi, and the degree of staining of the lipid droplets was confirmed under a microscope. The images obtained by the microscope were analyzed with Image J, and the values are shown in Fig. 1.

As shown in Figure 1, it was confirmed that sebum production was increased by the inducer, palmitic acid, and that sebum production was effectively suppressed when treated with the extract of turmeric and the positive control, EGCG.

Example 3: Safety test of the extract of turmeric on human skin

In order to verify whether the tulsi extract according to the present invention is safe for human skin, a skin safety verification experiment was performed. For this purpose, a skin cumulative irritation test was performed. Specifically, formulations containing 0.1%, 0.5%, and 1% of the tulsi extract of Example 1 were manufactured, and these were used to apply patches to the upper arm area of 30 healthy adults every other day for a total of 9 24-hour cumulative patches to measure whether the tulsi extract irritates the skin. A Finn chamber (Finn chamber, Epitest Ltd, Finland) was used for the patch application. 15 ㎕ of each of the above skin external agents was dropped into the chamber, and then the patch application was performed. The degree of the reaction shown on the skin each time was scored using the following mathematical formula 1, and the results are shown in Table 1 below.

[Mathematical formula 1]

Average Response = [[{(Response Index x Response) / (Total Number of Examiners x Highest Score (4 points))}] x 100] ÷ Number of Examinations (9 times)

In the reactivity scale, ± is given 1 point, + is given 2 points, and ++ is given 4 points, and a composition is considered safe when the average reactivity is less than 3.

Test substance Number of subjects who responded average
Reaction rate 1 week 2 weeks 3 weeks 1st 2nd 3rd 4th 5th 6th 7th 8th 9th ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ ± ＋ ＋＋ Control group
(Squalene) 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Hot
extract
(0.1%)
[Test group 1] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Hot
extract
(0.5%) [Test group 2] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Hot
extract
(1%)
[Test group 3] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00

As shown in Table 1 above, the number of people corresponding to ±, + and ++ in test groups 1, 2 and 3 was all 0, and the average reaction rate was also 0.00. Since the test results showed an average reaction rate of 3 or less, the thuja extract according to the present invention was determined to be a safe substance for human skin that does not show a distinct cumulative irritation pattern.

The composition of the present invention can be prepared in the following formulations, but is not limited thereto.

Formulation Example 1: Cosmetic Formulation

1-1. Flexible toner

As shown in Table 2 below, a flexible toner containing the extract of Example 1 as an effective ingredient was manufactured according to a conventional method.

ingredient weight% Tofu extract 0.01 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Ethanol 10.0 Triethanolamine 0.1 Preservatives, trace colors, trace flavors and trace purified water 82.79 aggregate 100.0

1-2. Nourishing Toner

As shown in Table 3 below, a nutritious toner containing the extract of Example 1 as an effective ingredient was manufactured according to a conventional method.

ingredient weight% Tofu extract 0.01 beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic/Capric Triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservatives, trace colors, trace flavors and trace purified water 69.69 aggregate 100.0

1-3. Nourishing cream

A nutritional cream containing the extract of the plant of Example 1 as an effective ingredient, as shown in Table 4 below, was manufactured according to a conventional method.

ingredient weight% Tofu extract 0.01 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic/Capric Triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, trace colors, trace flavors and trace purified water 56.79 aggregate 100.0

1-4. Massage Cream

A massage cream containing the extract of the plant of Example 1 as an effective ingredient, as shown in Table 5 below, was manufactured according to a conventional method.

ingredient weight% Tofu extract 0.01 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic/Capric Triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, trace colors, trace flavors and trace purified water 27.49 aggregate 100.0

1-5. Pack

A pack containing the extract of the plant of Example 1 as an effective ingredient, as shown in Table 6 below, was manufactured according to a conventional method.

ingredient weight% Tofu extract 0.01 polyvinyl alcohol 13.0 Sodium carboxymethyl cellulose 0.2 Allantoin 0.1 Ethanol 5.0 Nonylphenyl ether 0.3 Preservatives, trace colors, trace flavors and trace purified water 81.39 aggregate 100.0

1-6. Shampoo

A shampoo containing the extract of the plant of Example 1 as an effective ingredient was manufactured according to a conventional method, as shown in Table 7 below.

ingredient weight% Tofu extract 0.01 TEA-Cocoyl Glutamate 10.0 Cocamidopropyl Betaine 20.0 glycerin 5.0 Panthenol 0.05 Niacinamide 0.2 Xanthan gum 1.0 Preservatives, trace colors, trace flavors and trace purified water 63.74 aggregate 100.0

1-7. Hair Serum

A hair serum containing a turmeric extract as an active ingredient was manufactured according to a conventional method, as shown in Table 8 below.

ingredient weight% Tofu extract 0.01 menthol 0.1 Polysorbate 20 0.5 Ethanol 5.0 Panthenol 0.1 Niacinamide 0.3 Pyridoxine HCL 0.1 Dipropylene glycol 2.0 Preservatives, trace colors, trace flavors and trace purified water 91.89 aggregate 100.0

Preparation Example 2: Pharmaceutical preparation

2-1. Manufacturing of Sanje

ingredient Content (g) Tofu extract 2 Lactose 1

The above ingredients were mixed and filled into a sealed bag to prepare a powdered preparation.

2-2. Manufacturing of tablets

ingredient Content (mg) Tofu extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

After mixing the above ingredients, tablets were manufactured by pressing them according to the usual method for manufacturing tablets.

2-3. Manufacturing of capsules

ingredient Content (mg) Tofu extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

After mixing the above ingredients, the mixture was filled into a gelatin capsule according to a conventional capsule manufacturing method to produce a capsule.

2-4. Preparation of gel

ingredient weight% Tofu extract 0.01 Butylene glycol 2.00 glycerin 1.00 Disodium EDTA 0.02 PEG-60 Hydrogenated Castor Oil 1.00 Cocamidopropyl Betaine 4.00 Lauryl Glucoside 6.00 Polysorbate 20 1.00 Sodium benzoate 0.40 Caprylic Glycol 0.20 Sodium lactate 0.50 Hydroxyethyl cellulose 0.7 Purified water 83.17 aggregate 100.0

2-5. Preparation of ointment

ingredient weight% Tofu extract 0.01 diethanolamine 1.5 Polyvinyl photolidone 5.0 Propylene glycol 30.0 Purified water 63.49 aggregate 100.0

Claims (9)

A cosmetic composition for suppressing sebum secretion containing a turmeric extract as an effective ingredient,
A cosmetic composition characterized in that the above-mentioned thuja extract is prepared by a method including: (S10) a step of hot water extracting thuja; (S20) a step of immersing the above-mentioned thuja hot water extract in an organic solvent; and (S30) a step of obtaining only a precipitate from the immersion material. delete A cosmetic composition according to claim 1, characterized in that the cosmetic composition is for preventing or improving seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema by suppressing sebum secretion. A pharmaceutical composition for preventing or treating seborrheic dermatitis, seborrheic dermatitis, folliculitis, or seborrheic eczema, comprising a turmeric extract as an active ingredient.
A pharmaceutical composition characterized in that the above-mentioned thuja extract is prepared by a method including: (S10) a step of hot water extracting thuja; (S20) a step of immersing the above-mentioned thuja hot water extract in an organic solvent; and (S30) a step of obtaining only a precipitate from the immersion material. delete A composition for external application of skin for the prevention, improvement or treatment of seborrheic dermatitis, seborrheic dermatitis, folliculitis or seborrheic eczema, containing a turmeric extract as an effective ingredient.
A skin external application composition characterized in that the above-mentioned thuja extract is manufactured by a method including: (S10) a step of hot water extracting thuja; (S20) a step of immersing the above-mentioned thuja hot water extract in an organic solvent; and (S30) a step of obtaining only a precipitate from the immersion material. delete A food composition for controlling sebum secretion containing a turmeric extract as an effective ingredient,
A food composition characterized in that the above-mentioned thuja extract is prepared by a method including: (S10) a step of hot water extracting thuja; (S20) a step of immersing the above-mentioned thuja hot water extract in an organic solvent; and (S30) a step of obtaining only a precipitate from the immersion material. delete