KR102348283B1 - 멀티앱타머 표적 검출 - Google Patents

멀티앱타머 표적 검출 Download PDF

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KR102348283B1
KR102348283B1 KR1020167006262A KR20167006262A KR102348283B1 KR 102348283 B1 KR102348283 B1 KR 102348283B1 KR 1020167006262 A KR1020167006262 A KR 1020167006262A KR 20167006262 A KR20167006262 A KR 20167006262A KR 102348283 B1 KR102348283 B1 KR 102348283B1
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aptamer
deoxyuridine
target
complex
carboxyamide
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KR20160058777A (ko
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우르스 에이. 옥스너
루이스 에스. 그린
래리 골드
네보쟈 야닉
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소마로직, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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KR1020167006262A 2013-09-24 2014-09-24 멀티앱타머 표적 검출 Active KR102348283B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361881629P 2013-09-24 2013-09-24
US61/881,629 2013-09-24
PCT/US2014/057143 WO2015048084A1 (en) 2013-09-24 2014-09-24 Multiaptamer target detection

Publications (2)

Publication Number Publication Date
KR20160058777A KR20160058777A (ko) 2016-05-25
KR102348283B1 true KR102348283B1 (ko) 2022-01-10

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US (2) US9926566B2 (https=)
EP (1) EP3049523B1 (https=)
JP (1) JP6506768B2 (https=)
KR (1) KR102348283B1 (https=)
CN (2) CN114703193A (https=)
AU (1) AU2014326975B2 (https=)
BR (1) BR112016005957A2 (https=)
CA (1) CA2924987C (https=)
HK (1) HK1218765A1 (https=)
IL (1) IL244284B (https=)
MX (1) MX361451B (https=)
SG (1) SG11201602063UA (https=)
WO (1) WO2015048084A1 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6506768B2 (ja) 2013-09-24 2019-04-24 ソマロジック・インコーポレーテッド 多重アプタマー標的の検出
TWI695068B (zh) 2014-11-24 2020-06-01 美商身體邏輯股份有限公司 用於結合生長分化因子１１的核酸化合物
PL3387152T3 (pl) * 2015-12-08 2022-05-09 Twinstrand Biosciences, Inc. Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego
JP6853265B2 (ja) 2016-03-14 2021-03-31 ソマロジック, インコーポレイテッドSomaLogic, Inc. 核酸に組み込むための５−（ｎ−保護−トリプタミノカルボキシアミド）−２’−デオキシウリジンホスホロアミダイトの化合物及び合成方法
KR20190017870A (ko) * 2016-06-17 2019-02-20 프록시머티 바이오사이언시스, 엘엘씨 앱타머 쌍 선택 방법
TWI770036B (zh) 2016-07-01 2022-07-11 美商私募蛋白質體操作有限公司 包含經修飾核苷之寡核苷酸
DK3548652T3 (da) 2016-12-01 2024-07-08 Nautilus Subsidiary Inc Fremgangsmåder til proteinanalyse
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
EP3810563A4 (en) * 2018-06-22 2022-03-23 Somalogic, Inc. IMPROVED PROTEOMIC MULTIPLEX TESTS
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
EP4281774B1 (en) 2021-01-20 2025-11-26 Nautilus Subsidiary, Inc. Biomolecule quantitation
EP4281775B1 (en) 2021-01-21 2026-04-29 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
CA3227592A1 (en) 2021-09-22 2023-03-30 Gregory KAPP Methods and systems for determining polypeptide interactions
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
WO2024107857A1 (en) 2022-11-15 2024-05-23 Nautilus Subsidiary, Inc. Standard polypeptides
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
US20240201182A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024216233A1 (en) 2023-04-13 2024-10-17 Nautilus Subsidiary, Inc. Artificial proteins for displaying epitopes
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2026073213A1 (en) * 2024-09-30 2026-04-02 Illumina, Inc. Protein capture techniques with surface linked aptamers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120231467A1 (en) * 2011-03-10 2012-09-13 Somalogic, Inc. Aptamers for C. Difficile Diagnostics

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
US7312325B2 (en) * 2000-09-26 2007-12-25 Duke University RNA aptamers and methods for identifying the same
US20070071744A1 (en) * 2002-06-07 2007-03-29 Gotz Munch Agents which bind to epitopes of glycoprotein VI
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US20100035247A1 (en) 2005-11-04 2010-02-11 U.S. Genomics, Inc. Heterogeneous Assay of Analytes in Solution Using Polymers
US8658670B2 (en) 2006-09-26 2014-02-25 Crestone, Inc. Methods and compounds for treatment of clostridium based infection
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
DK2172566T4 (da) * 2007-07-17 2022-06-13 Somalogic Inc Fremgangsmåde til generering af aptamerer med forbedrede off-rates
WO2009037264A2 (en) 2007-09-17 2009-03-26 Da Volterra Colonic delivery of antimicrobial agents
US8569252B2 (en) 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
WO2011100443A1 (en) 2010-02-11 2011-08-18 Intelligent Medical Devices, Inc. Oligonucleotides relating to clostridium difficile genes encoding toxin b, toxin a, or binary toxin
GB201002627D0 (en) 2010-02-16 2010-03-31 Loxbridge Res Llp Aptamer based analyte detection method
EP3345915B1 (en) * 2010-04-12 2021-11-24 Somalogic, Inc. 5-position modified pyrimidines and their use
US8785132B2 (en) 2010-04-23 2014-07-22 Postech Academy-Industry Foundation Aptamer sandwich assays
US20150031585A1 (en) 2011-03-10 2015-01-29 Somalogic, Inc. Multiaptamer Target Detection
KR20140044324A (ko) * 2011-04-26 2014-04-14 레가도 바이오사이언스, 인코포레이티드 페길화된 올리고뉴클레오티드의 제조방법
MX373248B (es) 2011-09-30 2020-05-11 Somalogic Operating Co Inc Prediccion de riesgo de evento cardiovascular y usos del mismo.
EP2798085B1 (en) 2011-12-30 2018-01-24 Quest Diagnostics Investments Incorporated Aptamers and diagnostic methods for detecting the egf receptor
JP6506768B2 (ja) 2013-09-24 2019-04-24 ソマロジック・インコーポレーテッド 多重アプタマー標的の検出

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120231467A1 (en) * 2011-03-10 2012-09-13 Somalogic, Inc. Aptamers for C. Difficile Diagnostics

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Gold et al., PLOS ONE, Vol.5, Issue 12, e15004, pp.1-17(2010.12.)*

Also Published As

Publication number Publication date
EP3049523B1 (en) 2019-08-07
CA2924987C (en) 2022-08-16
MX2016003320A (es) 2016-05-31
IL244284B (en) 2019-12-31
HK1218765A1 (zh) 2017-03-10
IL244284A0 (en) 2016-04-21
MX361451B (es) 2018-12-06
EP3049523A1 (en) 2016-08-03
CN114703193A (zh) 2022-07-05
US20160237435A1 (en) 2016-08-18
US20180187197A1 (en) 2018-07-05
AU2014326975A1 (en) 2016-04-07
US10392621B2 (en) 2019-08-27
JP6506768B2 (ja) 2019-04-24
BR112016005957A2 (pt) 2017-09-26
WO2015048084A1 (en) 2015-04-02
AU2014326975A2 (en) 2017-01-05
KR20160058777A (ko) 2016-05-25
CA2924987A1 (en) 2015-04-02
SG11201602063UA (en) 2016-04-28
EP3049523A4 (en) 2017-05-17
JP2016531593A (ja) 2016-10-13
US9926566B2 (en) 2018-03-27
CN105555957A (zh) 2016-05-04
AU2014326975B2 (en) 2020-05-07

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