KR102178779B1 - Composition for regulating skin hypersensitivity comprising Callophyllis japonica extract - Google Patents

Abstract

It relates to a composition for controlling skin hypersensitivity reactions containing red rice leaf extract, wherein the red rice leaf extract according to one aspect exhibits low cytotoxicity and inhibits secretion of excellent histamine and β-hexosaminidase in human mast cells. Since it has an effect, it can be safely used in cosmetics, medicines, foods, etc. to improve or treat allergic skin diseases including atopic dermatitis.

Description

Composition for regulating skin hypersensitivity comprising red crested leaf extract {Composition for regulating skin hypersensitivity comprising Callophyllis japonica extract}

It relates to a composition for controlling skin sensitization reactions comprising a red crested leaf extract.

Atopic dermatitis is accompanied by chronic skin inflammation and severe itching (pruritus). Itching, which arouses the feeling of wanting to scratch, is induced by itching mediating substances such as histamine.In the case of normal people, the symptoms are easily relieved when scratching, but in the case of atopic patients, the more scratched, the more the feeling of wanting to scratch is formed, causing severe scratching. Due to this scratching behavior of atopic patients, the skin barrier collapses and secondary infection is caused, which further exacerbates the inflammation. Atopic dermatitis can be temporarily relieved, but it recurs and worsens due to irritants such as the environment and food, and it is a phenomenon that deterioration and remission are repeated. Therefore, in order to improve skin diseases such as atopic dermatitis, a material that can effectively relieve itching is needed.

Human mast cells are cells that play a key role in causing acute and chronic inflammation in most allergic diseases including atopic dermatitis. The activities of histamine and β-hexosaminidase secreted by human mast cells are It is a major factor causing atopic dermatitis. When mast cells are activated by an overactive immune reaction, it secretes pruritogen such as histamine, which causes the skin barrier to collapse. Therefore, if a substance that can minimize side effects and suppress itching mediators is discovered, it will be effective in controlling various allergic diseases including atopic dermatitis.

The red crested leaf ( Callophyllis japonica ) belongs to the Calimenia family, is one of the seaweeds classified as red algae. It is widely distributed in the south coast of Korea, and has been consumed as a food in Korea since ancient times. As previously reported, red crested leaves are known to have protective effects, antioxidant effects, and antibacterial effects against radiation irradiation in mice (Non-Patent Document 1), but red crested leaves are known to protect mast cells in relation to atopic dermatitis. Research on its efficacy is insignificant.

Jin-Woo Jeong, Chan-Woo Jeong,Jeong-Tae Kim, Won-Joon Yang, ee-Jung Ahn, Byeoung-Hak Kim, Joo-Ah Kim, Taekyun Shin, Korean Society of Livestock Hygiene Vol. 32, No. 4, 2009 , 353-359)

One aspect is to provide a cosmetic composition for preventing or improving skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

Another aspect is to provide a pharmaceutical composition for preventing or treating skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

Another aspect is to provide a composition for external application for skin for preventing or treating skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

Another aspect is to provide a health functional food composition for preventing or improving skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

One aspect provides a cosmetic composition for preventing or improving skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

The "crested red leaf (Callophyllis japonica)" belongs to the Kallymeniaceae family, and is one of the seaweeds classified as red algae. Red crested leaves are distributed in the western coast of the North Pacific Ocean, for example, in the south coast of Korea, and may be grown in the lower intertidal zone and subtidal zone. The plant of the red crested leaves forms a leaf shape, and a short stem is formed from the small half-headed roots, and the branches are irregularly divided and spread in a wedge shape, which may increase by about 10 to 20 cm. Stems and branches are flattened, and there may be serrated projections on the edges.

The "crested red leaf extract" may be an extract extracted with a solvent from the whole, part of, or a material derived therefrom.

In one embodiment, the extract may be extracted using a hydrophilic solvent, such as water, alcohol, or a combination thereof as a solvent. The alcohol may be a compound having one or more -OH groups of C1 to C6. The alcohol may be methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, isobutanol, tert-butanol, n-pentanol, n-hexanol, or a mixture thereof. The solvent may be, for example, a mixture of water and alcohol, that is, an aqueous alcohol solution. The alcohol concentration of the aqueous alcohol solution is 1 to 99.5 (v/v)%, for example, 10 to 99.5 (v/v)%, 1 to 70 (v/v)%, 1 to 40 (v/v)%, It may be 5 to 25 (v/v)%, 7 to 20 (v/v)%, 5 to 25 (v/v)%, or 10 to 20 (v/v)%. The aqueous alcohol solution may be an aqueous ethanol solution.

The extract may be extracted by conventional methods in the art, such as warm extraction, pressurized extraction, ultrasonic extraction, hot water extraction, reflux cooling extraction, subcritical extraction, or supercritical extraction.

The extraction is 4 ℃ to 85 ℃, for example, 4 ℃ to 70 ℃, 4 ℃ to 60 ℃, 4 ℃ to 50 ℃, 4 ℃ to 40 ℃, 4 ℃ to 30 ℃, 10 ℃ to 85 ℃, 15 It may be carried out at ℃ to 85 ℃, 20 ℃ to 85 ℃, 10 ℃ to 50 ℃, 10 ℃ to 40 ℃, 10 ℃ to 35 ℃, 10 ℃ to 30 ℃, 20 ℃ to 30 ℃, or at room temperature.

The extraction time may be appropriately selected according to the selected temperature and extraction method. For example, the extraction time is 1 hour to 3 days, 1 hour to 2 days, 1 hour to 1 day, 1 hour to 18 hours, 1 hour to 12 hours, 3 hours to 3 days, 3 hours to 2 days, 3 Time to 1 day, 3 hours to 18 hours, 3 hours to 12 hours, 6 hours to 3 days, 6 hours to 2 days, 6 hours to 1 day, 6 hours to 18 hours, 6 hours to 12 hours, or 6 hours To 9 hours.

The extraction may include mixing all of the red rice leaves, a portion thereof, or a material derived therefrom in the solvent and leaving it for a certain time. The standing may include suitable agitation.

The extraction may be one or more times, for example, 1 to 5 times, 1 to 4 times, 1 to 3 times, 2 to 5 times, or 2 to 4 times, and each extraction is performed by the same method, It can be done in different ways.

The extraction may be performed by separating plant residues and extracts by known methods such as filtration. The extraction may also include removing the solvent from the obtained extract by known methods such as vacuum concentration. The extraction may also include preparing a dried extract by drying the obtained extract, such as lyophilization.

The extract is 0.001% to 80% by weight based on the total weight of the composition, for example, 0.01% to 60% by weight, 0.01% to 40% by weight, 0.01% to 30% by weight, 0.01% to 20% by weight %, 0.01 wt% to 10 wt%, 0.01 wt% to 5 wt%, 0.05 wt% to 60 wt%, 0.05 wt% to 40 wt%, 0.05 wt% to 30 wt%, 0.05 wt% to 20 wt%, 0.05 wt% to 10 wt%, 0.05 wt% to 5 wt%, 0.1 wt% to 60 wt%, 0.1 wt% to 40 wt%, 0.1 wt% to 30 wt%, 0.1 wt% to 20 wt%, 0.1 wt% % To 10% by weight, or 0.1% to 5% by weight.

The composition according to an embodiment may include a fraction of the extract as an active ingredient. The "fraction" refers to a substance in which the red rice leaf extract is divided into a part thereof, that is, a substance that has been fractionated. The fraction may be obtained by solvent fractionation. The solvent fractionation may include mixing the red rice leaf extract with a solvent and separating a substance present in the solvent. Conditions for the fractionation, such as temperature conditions, pressure conditions, time, amount or concentration of the solvent used, and stirring, may be the same as described for the extraction used to prepare the above-mentioned red rice leaf extract. The fractionation may be repeated one or more times, for example 1 to 5 times. Separating the fraction may be performed by a known method such as filtration. The fractionation may also include removing the solvent from the obtained fraction by known methods such as concentration under reduced pressure. The fractionation may also comprise concentrating and/or drying the obtained fractions. The concentration may be concentrated under reduced pressure. The drying may include vacuum drying, boiling drying, spray drying, room temperature drying, or freeze drying.

The term “skin allergic disease” may mean that symptoms appear on the skin among allergic diseases. The symptoms may be acute or chronic, and may be local or systemic symptoms in the skin.

In one embodiment, the skin allergic disease may be any one selected from the group consisting of atopic dermatitis, allergic dermatitis, acute and chronic skin inflammatory diseases, contact dermatitis, eczema, and urticaria, resulting from It may include a secondary disease. For example, the skin allergic disease may be atopic dermatitis and may include a secondary disease resulting from atopic dermatitis. Specifically, the secretion of histamine or β-hexosaminidase in mast cells is excessively increased due to an abnormal hypersensitivity immune reaction, causing skin itching or skin inflammatory reaction, and the skin barrier collapses as the deterioration and remission are repeated. It may be causing an infection.

In one embodiment, the composition may inhibit the secretion of histamine by mast cells. For example, it may be to suppress an increase in histamine secretion of mast cells due to an overactive immune response mechanism such as atopic dermatitis. The histamine is a substance that is expressed in association with an inflammatory or allergic reaction, and may be a substance that causes itching.

In one embodiment, the composition may be to inhibit the secretion, expression, or activity of β-hexosaminidase (β-hexosaminidase). For example, it may be to inhibit the increase in the secretion, expression, or activity of β-hexosaminidase in mast cells by a mechanism of an overactive immune response such as atopic dermatitis. The β-hexosaminidase (β-HEX) is a substance expressed in association with inflammation or allergic reactions, and N-acetyl-β-D-hexosaminidase of mast cells It may be an enzyme involved in hydrolyzing acetyl-D-hexosamine.

The term “prevention” may comprehensively mean that the composition is administered in a pharmaceutically effective amount to prevent skin allergic diseases such as atopic dermatitis in advance, or to reduce the likelihood or frequency of occurrence. For example, it may be to lower the probability of onset or to lower the probability of recurrence in a patient with a possibility of developing a skin allergic disease such as atopic dermatitis or a patient who has had it. The term "improvement" may comprehensively mean alleviating skin conditions caused by skin allergic diseases such as atopic dermatitis by administering the composition in a pharmaceutically effective amount, and skin allergic diseases in a shorter time compared to natural healing. It may be to provide relief or cure of the symptoms of, and may be to improve one symptom or most of the symptoms caused by skin allergic diseases such as atopic dermatitis. The "pharmaceutically effective amount" refers to the type of disease, the patient's age, weight, health, sex, the patient's sensitivity to the drug, the route of administration, the method of administration, the number of administrations, the duration of treatment, the combination, or drugs used simultaneously. It can be easily determined by a person skilled in the art according to factors well known to. When administered to an individual, the "effective amount" may mean an amount that inhibits histamine secretion of mast cells, suppresses epithelial tissue hypertrophy, inhibits mast cell infiltration into a skin lesion, or reduces a skin lesion site. . The "individual" may be a mammal, for example, a human, cow, horse, pig, dog, sheep, goat, or cat, and may be a cell, tissue, organ, etc. derived from a mammal.

The “cosmetic composition” can be prepared in various forms, and in one embodiment, the composition is an emulsion, lotion, cream (oil-in-water type, water-in-oil type, multi-phase), solution, suspension (anhydrous and water-based), It may be any one formulation selected from the group consisting of emulsion, anhydrous product (oil and glycol type), paste, gel, mask, pack, powder, soap, powder, and spray.

The cosmetic composition may further include an acceptable carrier in a cosmetic formulation. The "acceptable carrier in cosmetic formulations" is a compound or composition that is already known and used that can be included in a cosmetic formulation, or is a compound or composition to be developed in the future, which does not have toxicity, instability or irritation beyond which the human body can adapt when in contact with the skin. Say that. The carrier may be included in the cosmetic composition of the present specification in about 1% by weight to about 99.99% by weight, preferably about 90% by weight to about 99.99% by weight, based on the total weight of the cosmetic composition. Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizing agents, viscosity modifiers, emulsions, stabilizers, sunscreen agents, color developing agents, fragrances, and the like.

Another aspect provides a pharmaceutical composition for preventing or treating skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

In the pharmaceutical composition, red crested leaves, red crested leaves extract, extract, extract, skin allergic diseases, and prevention are as described above.

The term "treatment" may comprehensively mean improving skin allergic diseases such as atopic dermatitis by administering the composition in a pharmaceutically effective amount, and alleviating symptoms of skin allergic diseases in a shorter time compared to natural healing. Or it may be to provide a cure, and may be to improve one symptom or most of the symptoms caused by skin allergic diseases such as atopic dermatitis. The pharmaceutically effective amount and the effective amount are as described above.

The "pharmaceutical composition" may be formulated in an oral or parenteral dosage form. The oral dosage form may be a granule, powder, liquid, tablet, capsule, dry syrup, or a combination thereof. The parenteral dosage form may be an injection or an external preparation for skin.

In one embodiment, the composition may further include a pharmaceutically acceptable carrier or excipient. The "carrier or excipient" may include any physiologically compatible solvent, dispersion medium, coating, antibacterial and antifungal agent, isotonic and absorption delaying agent. For example, lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl Cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, preservatives, and the like.

Another aspect provides a composition for external application for skin for preventing or treating skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

In the composition for external application for skin, red rice leaves, red rice leaves extract, extract, extract, skin allergic disease, prevention or treatment are as described above.

The "external preparation for skin" may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The above skin external preparations are ingredients commonly used in external preparations for skin such as cosmetics and pharmaceuticals, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances. , Colorants, various skin nutrients, or combinations thereof and may be appropriately formulated according to need. The external preparations for skin include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glavidine, and caline. Hot water extract of fruit, various herbal medicines, drugs such as tocopherol acetate, glytilithic acid, tranexamic acid and derivatives or salts thereof, vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars such as trehalose can also be appropriately blended.

Another aspect provides a health functional food composition for preventing or improving skin allergic diseases comprising an extract of Callophyllis japonica as an active ingredient.

In the health functional food composition, red rice leaves, red rice leaves extract, extract, extract, skin allergic disease, prevention or improvement are as described above.

The "health functional food composition" may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the health functional food composition may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the kind of the health functional food. Among the types of health functional foods, the beverage composition may contain various flavoring agents or natural carbohydrates as an additional component, like a conventional beverage. The natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumatin and stevia extract, and synthetic sweeteners such as saccharin and aspartame can be used. The health functional food composition may also include nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverage It may contain a carbonation agent used for, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverage, flesh for the production of vegetable beverages, or a combination thereof.

Another aspect provides a method of preventing or treating a skin allergic disease in a subject comprising administering to the subject a pharmaceutically effective amount of the pharmaceutical composition.

In the method of preventing or treating, a pharmaceutically effective amount, a pharmaceutical composition, an individual, a skin allergic disease, and prevention or treatment are as described above.

The term “administration” can be administered by methods known in the art. The administration can be administered directly to the subject by any means, for example by routes such as intravenous, intramuscular, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. . The administration can be administered systemically or locally. The administration may be topically administered to a site where an allergic skin disease exists.

The administration is 0.1 mg to 1,000 mg per individual, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg per individual, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg, 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg may be administered.

The red rice leaf extract according to one aspect exhibits low cytotoxicity and has excellent efficacy in inhibiting the secretion or activity of histamine and β-hexosaminidase in human mast cells, thus improving skin allergic diseases including atopic dermatitis. Or, it can be safely used in therapeutic cosmetics, medicines, and foods.

1 is a graph measuring the cell viability of human mast cells (HMC-1) according to the concentration of the red rice leaf extract.
FIG. 2 is a graph showing the amount of histamine secreted after the human mast cells (HMC-1) were stimulated with PMA to induce histamine secretion, and then treated with red crest leaf extract by concentration.
Figure 3 is a graph of measuring the amount of secretion of β-hexosaminidase after stimulating human mast cells (HMC-1) with PMA to induce the secretion of β-hexosaminidase, and then treating the red rice leaf extract by concentration to be.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1. Red crested leaves ( Callophyllis japonica ) Preparation of extract

Red crested leaves ( Callophyllis japonica ) were collected in winter in Wando, and washed three times with fresh water to remove seawater. The washed seaweed was dried in a dryer at 90° C. for 24 hours to remove moisture, and then made into a powder state using a blender. 100 g of seaweed powder was added to 900 ml of 70% ethanol, stirred, and filtered on a 3MM filter paper on the second day to collect the seaweed extract. This was repeated 3 times and extracted for a total of 6 days. The obtained extract was concentrated using a vacuum concentrator, and all solvents were removed using a freeze dryer to obtain a powdery extract. The obtained extract was dissolved in sterilized tertiary distilled water and ethanol and used for cell experiments.

Experimental Example 1. Cytotoxicity test of red rice red leaf extract

To evaluate the cytotoxicity of the red rice red leaf extract, the human mast cell line HMC-1 (human mast cells-1; hmc-1) was treated with red rice red leaf extract, followed by Annexin/PI staining, and the cells were subjected to flow cytometry. The survival rate was analyzed.

Specifically, Iscove's Modified Dulbecco's medium containing 10% fetal bovine serum (FBS), 200IU/ml penicillin, and 200g/ml streptomycin was added to HMC-1 cells, a human mast cell line. Medium; IMDM) at 37° C. and 5% CO ₂ . Human mast cells (HMC-1) cultured in a 25T flask were treated with red rice leaf extract at different concentrations (10-200 ug/ml) and cultured for 24 hours. After culturing for 24 hours, the cells were recovered and washed twice with PBS to remove the remaining medium. Washed cells were stained using Annexin V Apoptosis Detection Kit I (BD Biosciences). The stained cells were measured using an FC500 (Beckman coulter) instrument, and then analyzed using FlowJo V10 (FlowJo, LLC).

1 is a graph measuring the cell viability of human mast cells (HMC-1) according to the concentration of the red rice leaf extract.

As a result, as shown in FIG. 1, it was confirmed that neither of the groups treated with the red rice crest leaf extract exhibited cytotoxicity to human mast cells.

Experimental Example 2. Evaluation of Histamine Secretion Inhibition Effect of Red Crested Red Leaf Extract

In order to confirm the anti-atopic effect of the red rice leaf extract, HMC-1 cells were stimulated with PMA (phorbol 12-myristate 13-acetate; PMA) to induce histamine secretion, and then treated with red rice leaf extract. The efficacy of inhibiting histamine secretion was evaluated.

Specifically, the HMC-1 cells cultured in Experimental Example 1 were pretreated with red rice leaf extract for 2 hours, and then treated with PMA and calcium ionophore A23187. After centrifuging the cells and the medium, the supernatant was collected and the histamine secretion was measured using a Histamine ELISA kit (abcam).

FIG. 2 is a graph showing the amount of histamine secreted after the human mast cells (HMC-1) were stimulated with PMA to induce histamine secretion, and then treated with red crest leaf extract by concentration.

As a result, as shown in Figure 2, it was confirmed that the extract of red crested leaves reduced the secretion of histamine induced by PMA in a concentration-dependent manner. In particular, it was found that the amount of histamine secretion was reduced by about 60% or more compared to the group treated with PMA alone when the red rice leaf extract was treated at a concentration of 200 ug/ml. This means that the red crested red leaf extract is secreted by human mast cells and has the effect of inhibiting the secretion of histamine, which is a major factor causing atopic dermatitis, and thus can be usefully used in various skin allergic diseases including atopic dermatitis.

Experimental Example 3. Evaluation of the inhibitory efficacy of β-hexosaminidase secretion of red rice leaf extract

In order to confirm the anti-atopic effect of red rice leaf extract, β-hexose of red rice leaf extract was treated by stimulating HMC-1 cells with PMA to induce β-hexosaminidase secretion. The efficacy of inhibiting aminidase secretion was evaluated.

Specifically, the HMC-1 cells cultured in Experimental Example 1 were pretreated with red rice leaf extract for 2 hours, and then treated with PMA and calcium ionophore A23187. After centrifuging the cells and the medium, the supernatant was recovered, and the secretion amount of β-hexosaminidase was measured as follows. A culture solution of HMC-1 cells treated with red rice leaf extract, PMA, and calcium ionophore A23187 was reacted with the same amount of 1mM p-nitrophenyl-N-acetly-β-D-glucosaminide at room temperature for 1 hour, and then Na ₂ CO ₃ /NaHCO ₃ was treated with 250 μl and absorbance was measured at 400 nm with an ELISA plate reader.

Figure 3 is a graph of measuring the amount of secretion of β-hexosaminidase after stimulating human mast cells (HMC-1) with PMA to induce the secretion of β-hexosaminidase, and then treating the red rice leaf extract by concentration to be.

As a result, as shown in FIG. 3, it was confirmed that the extract of red rice leaves reduced the secretion of β-hexosaminidase induced by PMA in a concentration-dependent manner. In particular, it was found that when the red rice leaf extract was treated at a concentration of 200 ug/ml, the amount of β-hexosaminidase secretion was reduced by about 60% or more compared to the group treated with PMA alone. It has the effect of suppressing the secretion of β-hexosaminidase, which is a major factor that causes atopy and dermatitis, and is secreted from human mast cells, so it can be usefully used in various skin allergic diseases including atopic dermatitis. Means you can.

Claims (16)

A cosmetic composition for preventing or improving atopic dermatitis comprising an extract of red crested leaves ( Callophyllis japonica ) as an active ingredient. The cosmetic composition of claim 1, wherein the extract is extracted using water, C1-C6 alcohol, or a combination thereof as a solvent. delete The cosmetic composition of claim 1, wherein the composition inhibits histamine secretion of mast cells. The cosmetic composition of claim 1, wherein the composition inhibits the secretion, expression, or activity of β-hexosaminidase. The method according to claim 1, wherein the composition is any one formulation selected from the group consisting of emulsions, lotions, creams, solutions, suspensions, emulsions, pastes, gels, masks, packs, powders, soaps, powders, and sprays. Cosmetic composition. A pharmaceutical composition for preventing or treating atopic dermatitis comprising an extract of red crested leaves ( Callophyllis japonica ) as an active ingredient. The pharmaceutical composition of claim 7, wherein the extract is extracted using water, C1-C6 alcohol, or a combination thereof as a solvent. delete The pharmaceutical composition according to claim 7, wherein the composition further comprises a pharmaceutically acceptable carrier or excipient. A composition for external application for skin for preventing or treating atopic dermatitis comprising an extract of red crested leaves ( Callophyllis japonica ) as an active ingredient. The composition for external application for skin according to claim 11, wherein the extract is extracted using water, C1-C6 alcohol, or a combination thereof as a solvent. delete A health functional food composition for preventing or improving atopic dermatitis comprising an extract of red crested leaves ( Callophyllis japonica ) as an active ingredient. The health functional food composition of claim 14, wherein the extract is extracted using water, C1-C6 alcohol, or a combination thereof as a solvent. delete

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