KR102174084B1 - Cosmetic composition for skin improvement and skin regeneration using AFA blue green algae - Google Patents

Abstract

The present invention discloses a cosmetic composition for skin regeneration, and in the cosmetic composition for skin regeneration consists of an aqueous layer containing a peptide-based compound as an active component, and an oil layer containing MCT, the aqueous layer contains an AFA extract, and the oil layer contains flavonoids. The cosmetic composition contains the AFA extract and cytokines, thereby exhibiting various skin condition improvement effects such as alleviating wrinkles, improving skin elasticity, being antioxidant, preventing skin aging, alleviating striae distensae, healing wounds, relieving scars, alleviating age spots, being skin whitening, improving moisturizing, promoting hair growth, alleviating itching, etc.

Description

Cosmetic composition for skin improvement and skin regeneration using AFA blue green algae}

The present invention relates to a cosmetic composition for skin regeneration containing AFA extract, and more specifically, it contains AFA extract and cytokines, and improves moisture content to improve wrinkles, skin regeneration, skin elasticity, antioxidant, skin aging prevention , Stretch marks, wound healing, scar relief, age spot improvement, whitening, moisturizing improvement, hair growth promotion, itching improvement, etc. to a cosmetic composition that can exhibit various skin condition improvement effects.

Protein is one of the key compounds that make up the skin along with lipids except water, and it is known that about 58% of protein exists in the form of keratin in the stratum corneum. Proteins are closely related to the life phenomena and functioning of living organisms. The basic unit of protein is called amino acid and includes carbon, oxygen, hydrogen, nitrogen and/or sulfur and the like. Amino acids have a basic structure bonded to an amino group (*-NH2), a carboxyl group (*-COOH) and various functional groups (*-R) on a carbon atom. The kind of amino acid is determined by this functional group, and about 20 kinds of the functional group are known.

A bond is formed between the amino group of one amino acid and the carboxyl group of another amino acid as water escapes from one molecule, which is called a peptide bond. An amino acid chain in which a number of amino acids are linked through a peptide bond is called a polypeptide. Polypeptides have a folded or twisted form due to hydrogen bonds, hydrophobic interactions and disulfide bonds between side chains. A form in which a plurality of polypeptides are gathered through interaction and aggregated into one super-molecule form is generally referred to as a protein.

Proteins can be largely divided into a hydrophobic moiety and a hydrophilic moiety. Most proteins exhibit polarity due to the influence of hydrophilic moieties, and show high solubility in aqueous media. However, most hydrophilic proteins are known to be chemically unstable when stored for a long time. For example, the possibility of an additional condensation reaction between a plurality of hydrophilic proteins, the progress of a decomposition reaction by a solvent, and high oxidation induced by a plurality of hydrogen bonds may be problematic. Accordingly, there are many restrictions on the use of protein compounds in cosmetics for improving skin feeling or supplying physiologically active ingredients.

Particularly, when a hydrophilic protein or polypeptide is included as a physiologically active component in a cosmetic, structural and chemical denaturation of the protein or polypeptide is likely to occur by a surfactant, oil, acid or base catalyst. Therefore, cosmetic products containing hydrophilic proteins have problems such as deterioration of the appearance and deterioration of functions due to denaturation of physiologically active ingredients over time.

In order to prevent the denaturation of the hydrophilic protein as described above and to promote its use as a physiologically active substance, Patent Document 1 discloses a protein particle prepared through crosslinking of the amino acid side branches constituting the protein by forming an acylation polyvalent crosslinking agent on the vegetable protein. It is developed and released. However, the chemical structure of the protein is greatly damaged in that the protein is chemically denatured and granulated through a crosslinking agent, and in fact, it is not preferable in that it is difficult to reproduce the chemical properties of the protein before the crosslinking reaction.

Meanwhile, in Patent Document 2, in order to suppress the continuous denaturation of the water-soluble protein contained in the cosmetic as described above, the water-soluble protein is pretreated and transformed into water-insoluble particles. However, this approach has the advantage of suppressing further denaturation of the water-soluble protein by the solvent, but in reality, the denaturation must be made before the use of the protein, so that the function expected as a physiologically active substance will be expressed identically. It has a fatal limitation of being difficult to expect.

Korean Patent Publication KR 10-0505322 B1 (2005.07.25) Republic of Korea Patent Publication KR 10-2017-0066260 (2017.06.14)

The present invention has been developed to solve the above-described technical problem, and an object of the present invention is to provide a cosmetic composition for skin regeneration containing a functional peptide.

In addition, it is an additional object of the present invention to provide a cosmetic composition for skin regeneration with limited denaturation of a water-soluble protein or functional peptide.

In addition, it is an additional object of the present invention to provide a cosmetic composition for skin regeneration in which denaturation of water-soluble proteins by preservatives may not occur.

On the other hand, the present invention has an additional object to provide a cosmetic composition for skin regeneration that can additionally provide chemical energy consumed for skin regeneration.

As a result of researching in order to solve the above-described problems, the present inventors have come up with an invention including the following configuration. Specifically, in the present specification, in the cosmetic composition for skin regeneration consisting of an aqueous layer comprising a peptide-based compound as an active ingredient and an oil layer including MCT, the aqueous layer includes AFA extract, and the oil layer includes flavonoids, Disclosed is a cosmetic composition for skin regeneration.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, it is preferable that the peptide-based compound further contains cytokines as an active ingredient.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, it is preferable that the oil phase layer further comprises at least one polyunsaturated fatty acid selected from the group consisting of omega-3, omega-6, and omega-9.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, the cytokine is preferably one or more growth factors selected from the group consisting of EGF, VEGF, FGF, AFGF, IGF, and KGF.

In addition, in each cosmetic composition for skin regeneration of the present invention, it is preferable that the flavonoid is a flavone-based compound.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, the flavone-based compound is composed of chrysin, tectochrysin, primetin, apigenin, and acetin. It is preferable that it is at least one selected from the group.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, when the weight of the AFA extract is 100, the weight ratio of cytokines contained in the aqueous phase of the present invention is preferably between 1 and 0.04, and between 0.1 and 0.05. It is more preferable.

In addition, in each cosmetic composition for skin regeneration of the present invention, when the total weight of the oil phase is 100, the flavone-based compound contained in the oil phase is preferably between 2.5 parts by weight and 20 parts by weight.

Through the adoption of the above-described means, the present invention can provide a cosmetic composition for skin regeneration containing a functional peptide.

In addition, the aqueous layer of the present invention may limit the denaturation of the water-soluble protein or functional peptide by inhibiting oxidation through the shielding of oxygen without additionally containing a preservative.

In addition, the aqueous layer of the present invention may limit the degeneration of AFA extract or cytokine by limiting the Maillar reaction between additives without additionally containing a preservative.

On the other hand, the cosmetic composition of the present invention may additionally provide chemical energy consumed in skin regeneration by separately providing an oily layer containing MCT and unsaturated fatty acid.

The terms used in the present application are only used to describe specific examples. So, for example, a singular expression includes a plural expression unless the context clearly has to be singular. In addition, terms such as "include" or "include" used in the present application are used to clearly refer to the existence of features, steps, functions, components or combinations thereof described in the specification, but other features It should be noted that it is not used to preliminarily exclude the presence of elements, steps, functions, components, or combinations thereof.

Meanwhile, unless otherwise defined, all terms used in this specification should be viewed as having the same meaning as commonly understood by a person of ordinary skill in the art to which the present invention belongs. Therefore, unless clearly defined in the specification, specific terms should not be interpreted in an excessively ideal or formal sense.

In the present specification, in the cosmetic composition for skin regeneration consisting of an aqueous layer containing a peptide-based compound as an active ingredient and an oil phase layer including MCT, the aqueous layer contains AFA extract, and the oil phase layer contains flavonoids, skin regeneration Disclosed is a cosmetic composition for use.

Aphanizomenon flos-aquae (AFA) is a blue-green algae known to contain a variety of antioxidants and other biologically active compounds. When AFA extract is applied to the skin, it is known that adult skin fibroblasts proliferate significantly.

"AFA extract" refers to any fraction, extract, or all isolated or purified molecule from Aphanizomenon flos aquae. However, as an example, the AFA extract is preferably a protein or nucleic acid of Aphanizomenon flos aquae, or a phytochemical, or fraction, excluding reducing saccharides. For example, reducing saccharides in the extract of AFA can be separated and removed from the AFA extract by extracting AFA with about 80% ethanol at 60° C. for 3 hours.

As a feature of the present invention, the AFA extract is mixed with a peptide-based compound to be described later in a form in which reducing saccharides are excluded. Both are dissolved in the aqueous layer of the cosmetic composition for skin regeneration of the present invention. In particular, as a specific example of the peptide-based compound, when a growth factor such as EGF is dissolved with a reducing saccharide, denaturation and loss of function of the aspartic acid moiety may occur due to Maillard reaction or the like. . Therefore, the AFA extract is preferably used in a form in which reducing sugars are excluded.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, it is preferable that the peptide-based compound further contains cytokines as an active ingredient. In addition, in each of the cosmetic compositions for skin regeneration of the present invention, cytokines are EGF (epidermal growth factor), AFGF (anti-aging growth factor), VEGF (vascular endothelial growth factor), FGF (fibroblast growth factor), IGF (Insulin-like growth factor), it is preferably at least one growth factor selected from the group consisting of KGF (keratinocyte growth factor). The growth factor can promote cell proliferation and differentiation by binding to receptors on the surface of the cell.

On the other hand, when the weight of the AFA extract is 100, the weight ratio of cytokines contained in the aqueous phase of the present invention is preferably between 1 and 0.04. When the cytokine is added in excess of 1, the Maillar reaction between the cytokine, more specifically, the amino acid (mainly the amine group of aspartic acid, etc.) contained in the growth factor at room temperature and the reducing sugar contained in a very small amount in the AFA extract As it proceeds excessively, degeneration, loss of function, and loss of aesthetics of the aqueous layer of the cosmetic composition of the present invention may be problematic. Conversely, when the content of cytokines is less than 0.04, sufficient functioning of growth factors and the sense of quality when used may be significantly limited.

In addition, when the weight of the AFA extract is 100 from the viewpoint of pursuing the maximum function without denaturation even under the temperature condition of 40°C, the weight ratio of cytokines contained in the aqueous phase of the present invention is 0.1 to More preferably between 0.05.

MCT (Middle-Chain Triglycerides) refers to a compound in which 3 equivalents of medium chain fatty acids are bound to the main chain of triglycerides. At this time, the medium chain fatty acid refers to a fatty acid of about C6 to C12. The medium chain fatty acids bound to the triglyceride main chain of MCT may or may not be the same as each other, and need not be the same. Details of the medium chain fatty acids contained in MCT can be adjusted by a person skilled in the art, but it is sufficient if it can function as a solvent for the substances mixed in the oil phase.

Medium chain fatty acids can be saturated or unsaturated, but preferably saturated. Examples of the medium chain fatty acids constituting the medium chain triglycerides of the present invention are C6 (capro fatty acid), C8 (caprylic fatty acid), C10 (capric fatty acid), and C12 (lauric fatty acid), and mixtures thereof. In one embodiment, the MCT comprises a mixture of about 60% C8 triglyceride and about 40% C10 triglyceride to about 70% C8 triglyceride and about 30% C10 triglyceride. It is believed that MCTs containing C8/C10 are rapidly absorbed into the skin.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, it is preferable that the oil phase layer further comprises at least one polyunsaturated fatty acid selected from the group consisting of omega-3, omega-6, and omega-9.

Omega-3 refers to a series of fatty acids in which the third carbon from the end of the methyl group of the fatty acid has a double bond, and DHA (Docosahexaenoic acid), HTA (Hexadecatrienoic acid), ALA (α-Linolenic acid), SDA (Stearidonic acid) ), ETE (Eicosatrienoic acid), EPA (Eicosapentaenoic acid), and DPA (Docosapentaenoic acid). In addition, omega-6 and 9 refer to a series of fatty acids having double bonds in the 6th and 9th carbons from the end of the methyl group of the fatty acid, respectively, and omega-6 is LA (Linoleic acid) and GLA (Gamma-linolenic acid). ), and other unsaturated fatty acids, and omega-9 contains polyunsaturated fatty acids such as oleic acid.

The polyunsaturated fatty acids mentioned above are olive oil, abyssinian oil, castor oil, jojoba oil, soybean oil, sunflower oil, macadamia nut oil, almond oil, rice bran oil, corn oil, grape seed oil, coconut oil, meadow foam seed. It can be extracted from one or more vegetable oils selected from the group consisting of oil, avocado oil, babasu oil, aloe oil, borage oil, coffee oil, heme seed oil and tea tree oil.

In addition, the polyunsaturated fatty acids described above can be obtained from fish oil derived from one or more types of cold water fish. Non-limiting examples of cold water fish include deep-sea fish, shark, salmon, cod, salmon, bonito, mackerel, Atlantic mackerel, haddock, herring, mahi mahi, menhadenm, mackerel, caplin. , Tilapia, Pacific saury, krill, anchovies, pollock, trout, whitefish, tuna, smelt, shad, and sardines, or combinations thereof.

In the cosmetic composition for skin regeneration of the present invention, polyunsaturated fatty acids become a constituent of cell membranes and intercellular lipids, thereby facilitating cell flexibility and inflow of internal and external substances. That is, by including an appropriate amount of polyunsaturated fatty acid in the oil layer, the cosmetic composition for skin regeneration of the present invention can impart flexibility and elasticity to the skin, prevents additional damage due to dehumidification of the wound area, and It can contribute to skin regeneration by contributing to reorganization.

In addition, polyunsaturated fatty acids applied to the skin along with MCT are enzymatically modified after being absorbed into the body to make compounds useful for normal body functions. Polyunsaturated fatty acids and MCT are known to be anti-inflammatory compounds, and may contribute to cell regeneration in wounds. Specifically, they reduce resistance in intradermal capillaries that drain the dermis. When swelling is reduced, surface circulation increases. On the other hand, the medium-chain fatty acid contained in the MCT of the present invention can be rapidly absorbed into the body and utilized for metabolism, and can function as a chemical energy source.

In addition, in each cosmetic composition for skin regeneration of the present invention, the flavonoid is preferably a flavone-based compound substituted with a carbonyl functional group. Typically, flavonoid-based compounds are known to have high affinity for active oxygen molecules. In each of the cosmetic compositions for skin regeneration of the present invention, it is believed that the flavonoid compounds are dissolved in the oil phase to inhibit the infiltration or activation of oxygen molecules.

In addition, in each of the cosmetic compositions for skin regeneration of the present invention, the flavone-based compound is selected from the group consisting of Chrysin, tectochrysin, Primetin, apigenin, and acetin. It is preferably more than one. The flavone-based compound additionally contains an oxygen atom in the form of a hydroxy functional group or ether in the form of a functional group, so that it does not exceed the polarity range so as to be dissolved in the oil phase, and the functionality as an antioxidant is enhanced. For example, a hydroxy functional group additionally substituted in the main chain of flobone can induce some activated oxygen molecules through hydrogen bonding, and the benzene ring activated by the hydroxy functional group effectively binds oxygen molecules through electrostatic interactions. can do.

In particular, from the viewpoint that the desired physical properties and chemical properties can be realized and productivity can be achieved at the same time, in each cosmetic composition for skin regeneration of the present invention, it is preferable that the flavone-based compound dissolved in the oil phase layer is chrysine. Therefore, the following description assumes that the flavone-based compound is chrysine.

On the other hand, in the oil phase of the present invention, when the total weight of the oil phase is 100, the flavone-based compound is preferably between 2.5 parts by weight and 20 parts by weight. When the above numerical range is satisfied, it is possible to enjoy oxygen permeation and antioxidant effects through the oil phase layer included in the cosmetic composition of the present invention, while at the same time seeking harmony in production cost. For example, in the oil phase of the present invention, oxidation may occur less when the flavone compound is included than when the flavone compound is absent, and when 2.5 parts by weight or more of the flavone compound is contained, the antioxidant effect is significantly generated. I can.

On the other hand, as a substance that is dissolved in the oil phase or aqueous layer of the present invention and can provide an additional antioxidant function within a range that does not limit the function of the present invention, vitamin A and derivatives thereof, vitamin B and derivatives thereof , L-ascorbic acid and derivatives thereof, vitamin D and derivatives thereof, vitamin E and derivatives thereof, dibutylhydroxytoluene, butylhydroxyanisole, superoxide dismutase, carotenoids, astaxanthin, molars And its derivatives, and glutathione and its derivatives, glutathione, β-carotene and its derivatives, ubikinol, hydrangea, turmeric, youngsil, echinacea, golden flower root, red pepper sprouts, baeja, dynasty grass, rice, rice bran, comfrey, Japanese pepper tree , Bodhi tree, peony, soybean, natto, tea, cloves, loquat trees, peonies, maroniers, rock stalks, rooibos, rosemary, spirulina, chlorella, green algae, and other plants or plant-derived ingredients that have antioxidant effects, Bilirubin, cholesterol, tryptophan, histidine, thiotaurine, hypotaurine, etc. can be considered.

On the other hand, as a substance that can be dissolved in the oil phase or aqueous layer of the present invention to provide an additional anti-wrinkle function within the scope of not limiting the function of the present invention, retinoic acid, pangamic acid, kinetine, ursolic acid, Turmeric X, spingosine derivatives, silicon, silicic acid, and N-methyl-L-serine can be considered.

On the other hand, as a substance that can be dissolved in the oil phase or aqueous layer of the present invention to provide an additional transdermal absorption promoting function within a range that does not limit the function of the present invention, α-hydroxy acid, β-hydroxy acid , Oleic acid, menthol, thymol, limonene, propylene glycol, glycols such as polyethylene glycol, azacycloalkane derivatives, etc. may be considered.

Meanwhile, the oil phase layer or the water phase layer of the present invention may additionally include the following materials in addition to the above-described materials. The following materials may be used in an appropriate amount by a person skilled in the art without limiting the function of the present invention. Alcohol such as water, ethanol, isopropanol, liquid paraffin, petrolatum, microcrystalline wax, squalene, ceramide, almond oil, olive oil, hydrogenated oil, castor oil, wood wax, palm oil, beeswax, lanolin, carnauba wax, palm oil Oil components such as lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, higher alcohols such as cholesterol, esters such as isopropyl myristic acid, myristyl myristic acid, and isopropyl palmitic acid, phosphoric acid or citric acid , Α-hydroxy acids such as malic acid, tartaric acid, lactic acid, succinic acid, inorganic or organic acids other than the above, including acetic acid, salts of these acids, inorganic or organic alkali agents such as sodium hydroxide, potassium hydroxide, triethanolamine, and salts thereof (pH regulator), fullerene and its derivatives, coloring agents such as iron sulfate, titanium yellow, casamine, thiamine, nicotinic acid amide, riboflavin, L-ascorbic acid, pyroquinoline quinone, carotenoids, ergosterol, vitamins such as tocopherol, naringin , Dangjeoni Na Rinjin, Photograph No. 101 (Puradoin), Photograph No. 301 (Takanaru), Photograph No. 401, photosensitizer such as furalumin, Red No. 104, Yellow No. 4, Yellow No. 5, Green 3 No. 1, Blue No. 1, Blue No. 2, Red No. 202, Red No. 226, Red No. 227, Red No. 230, Orange No. 206, Orange No. 207, Yellow No. 202, Green No. 201, Green No. 204, Blue No. 201, Tar pigments such as Green No. 205, synthetic coloring agents such as those obtained by rake of natural coloring agents such as carminic acid, lacaic acid, carusamine, burazirin, and kurosine, safflower pigment, gardenia pigment, purple root pigment, cortinyl pigment, turmeric Pigment, hongguk (紅麴) pigment, beet pigment, lac pigment, madder pigment, perilla pigment, red olive pigment, blush pigment, purple potato pigment, elderberry pigment, blueberry pigment, red pepper pigment, anato Natural coloring of anthraquinone-based, anthocyanin-based, chalcone-based, carotenoid-based pigments, including pigments, spirulina pigments, cacao pigments, tamarindo pigments, oyster pigments, high-volume pigments, and caramel pigments Ryo et al. are examples.

On the other hand, it is preferable that the cosmetic composition for skin regeneration of the present invention constitutes a two-layer system divided into an oil phase-water layer. In particular, since the oil phase layer is located on the upper portion of the aqueous layer, direct exposure of the aqueous layer may be further limited. The oil phase layer located on the upper part of the aqueous layer restricts the access of oxygen molecules, thereby inhibiting the oxidation of components, particularly peptide-based compounds, in the aqueous layer by activated oxygen molecules.

As another example of the present invention, the cosmetic composition for skin regeneration of the present invention may be provided by uniformly mixing the oil phase layer and the water phase layer. However, the use of a surfactant is additionally required in order to provide the oil phase layer and the aqueous phase layer uniformly mixed. In addition, it is preferable to use a nonionic surfactant from the viewpoint of contributing to the preservation of the active ingredient dissolved in the water layer.

For example, nonionic surfactants having the above-described properties are examples, sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyethylene glycol fatty acid ester, polyoxyethylene alkyl ether, poly Oxyethylene polyoxypropylene alkyl ether, polyoxyethylene castor oil, etc. may be considered. In particular, the use of glycerin fatty acid esters among the nonionic surfactants is most preferred in terms of showing high affinity with the oily layer of the cosmetic composition for skin regeneration of the present invention. In the case of glycerin fatty acid ester, it is preferably between about 0.0001% by weight to 10% by weight, and more preferably between 3% by weight and 7% by weight based on the total weight of the cosmetic composition for skin regeneration of the present invention.

{Examples and evaluation}

Hereinafter, what is claimed by the present specification will be described in more detail with reference to the accompanying embodiments. However, the examples and the like presented in the present specification may be modified in various ways by a person skilled in the art to have various forms, and the description of the present specification is not limited to the specific disclosure form, but the present invention It should be viewed as including all equivalents or substitutes included in the scope of the spirit and technology of In addition, the embodiments of the present invention and the like are presented to help those skilled in the art to understand more accurately, and some figures may be exaggerated or reduced and disclosed.

Preparation Example 1: Preparation of lotion (lotion)

The following <Table 1> describes the detailed composition of the cosmetic composition for skin regeneration of the present invention. Each value represents a relative weight ratio when the total weight of the cosmetic composition of the present invention is 100. In the case of the remaining components except for cytokines, numerical values below the decimal point were omitted. On the other hand, when the total weight of the cosmetic composition was 100, the total weight of the water phase was 80, and the total weight of the oil phase was 20. The total sum of each layer described above was realized through the addition of purified water in the case of an aqueous phase, and through the addition of MCT in the case of an oil phase.

Meanwhile, each layer was prepared separately, and the lotion of the present invention was prepared by adding an oily layer on top of the finally prepared aqueous layer. In each of the Examples and Comparative Examples, the aqueous phase was layered on the lower portion of the container, and the oil phase was layered on the upper portion of the aqueous layer.

Ingredients Example Comparative example - ingredient One 2 3 4 5 6 7 8 9 One 2 3 Awards Purified water Balance (to 80) Ethylene
Glycol 10 10 glycerin 10 10 AFA extract ^** 15 20 10 15 15 25 10 15 Cytokine ^*** .01 .01 .05 .001 .01 .01 .One .01 Paid ^* MCT Remaining amount (to 20) Omega-3 1.5 1.5 Omega-6 1.5 1.5 Chrysine 2 2 2 0.5 4 2 2 - Other corrector Appropriate amount Spices Appropriate amount

* The ratio of the total weight of the water phase and the total weight of the oil phase was constant at 8 to 2. ** AFA extract is an extract obtained by excluding reducing sugar according to the description

*** In Examples and Comparative Examples (when cytokines are included), the relative composition ratios of cytokines are shown in Table 2 below. The composition ratio is compared to the relative weight of each component

ingredient EGF VEGF FGF AFGF IGF KGF Subsidy 12 1.5 One One One One

Preparation Example 2: Preparation of cream (concentrated essence)

To each composition in Table 1, a glycerin fatty acid ester and a thickener (xanthan gum) were additionally added to the oil phase, and the oil phase and the aqueous phase were mixed and stirred at 3000 rpm to prepare a cream-formed cosmetic composition. When the total weight of the cosmetic composition is 100, about 3 parts by weight of the glycerin fatty acid ester was added, and an appropriate amount of a thickener and a surfactant were added.

Evaluation 1. Sensory evaluation

Sensory evaluation was conducted to examine the overall performance of the cosmetic composition for skin regeneration of the present invention. In order to perform the following sensory evaluation, pre-diagnosis of the skin condition, etc. was performed, and a suitable type of subject for this evaluation was selected. In detail, the composition of Example 1 was used for the sensory evaluation below. The total evaluation period was 6 weeks, and pre-evaluation (at 0), 1 week after use (1st), 2 weeks later (2nd), 4 weeks later (3rd), 6 weeks later (4th). lost. The evaluation items were divided into moisture, keratin improvement, and dermal density. The instruments used in the evaluation are as follows.

-Using a Corneometer CM825 (made in Germany), the average moisture content of the left and right cheeks of each subject was measured.

-Using black tape (made in Japan), the degree of keratin improvement of the left cheek of each subject was measured.

-Using DermaScan C (made in Denmark), the dermal density of the left cheek of each subject was measured.

A total of 20 subjects participated in this evaluation, and the characteristics of the subjects who participated in the test are as follows. The average age of the subjects was 35.1 years. In the pre-evaluation, each subject's skin type was 7 cases of dryness including normal to dryness, 5 cases of neutrality, and 8 cases of oilyness including stopness. According to the preliminary evaluation, the majority of the subjects (13 subjects) perceived that their skin condition was dry, and the exposure time to left ultraviolet rays was less than 1 hour in 10 cases, between 1 hour and 3 hours in 7 cases, and 3 hours or more in 3 Yes. In particular, with regard to the evaluation of the degree of aging of the skin, 18 people answered positively to the question'Do you feel that your skin is aged compared to the past?' (very yes-4 cases, yes-14 cases).

1) Moisture evaluation

The measurement results are shown in Table 3 below. It was found that moisture content increased significantly as the usage period of the cosmetic composition of the present invention increased. Each number is written to the second decimal place, and the following values are rounded off.

Tea medium Standard Deviation Standard error mean 0 53.66 1.81 8.20 One 55.31 1.94 8.51 2 58.48 1.58 7.85 3 62.12 1.44 7.71 4 65.39 1.38 7.48

2) Evaluation of keratin improvement

The degree of keratin improvement could be estimated by measuring the angular mass of each subject. As a result of the analysis, it was confirmed that the longer the use period of the cosmetic composition for skin regeneration of the present invention, the significantly decreased angular mass. Summarizing the test results are shown in Table 4 below. Each number is written to the second decimal place, and the following values are rounded off.

The specific test method is as follows. The stratum corneum was collected from each subject's test site using black tape. The keratin attached to each black tape was photographed with an enlargement of 800 times. The photographed file was converted into a bitmap image and analyzed to calculate the surface area of the keratin.

Tea medium Standard Deviation Standard error mean 0 13.94 2.98 0.78 One 12.86 2.64 0.73 2 12.14 2.58 0.70 3 10.26 2.18 0.64 4 7.91 1.97 0.58

3) Dermal density evaluation

Ultrasonography was performed to track changes in dermal density of each subject. According to the measurement results, it was found that the dermal density increased significantly (p-value <0.05) after the third time. Specific measurement results are shown in Table 5 below. Each number is written to the third decimal place, and the following values are rounded off.

Tea medium Standard Deviation Standard error mean 0 5.541 0.122 0.549 One 5.648 0.138 0.612 2 5.716 0.140 0.638 3 5.985 0.144 0.657 4 6.102 0.168 0.692

4) Survey evaluation

After using the cosmetic composition of the present invention, a 5-point evaluation (5 points very positive, 1 point very negative) for the following items was conducted at the 0th time, the 2nd time, and the 4th time. The results of the evaluation results are summarized in Table 6 below. Each value is an average value and is written to the second decimal place.

Evaluation results. The subjects experienced overall improvement in skin health. In particular, it was found that the positive evaluation for the improvement of skin texture and fine wrinkles was high. According to the evaluation results, it is judged that the cosmetic composition of the present invention can provide at least a subjective positive feeling to the user in relation to the skin regeneration effect due to wrinkle improvement or the like.

Item Tea 0 2 4 My skin became healthy 3.20 3.45 4.05 Increased skin gloss 3.35 3.40 3.90 My skin color became brighter 3.30 3.60 3.95 Skin texture improved 3.35 3.60 4.05 The skin became soft 3.30 3.65 4.10 Fine wrinkles decreased 3.30 3.70 4.30 Reduced moisture 2.95 2.60 1.60

Evaluation 2. Stability evaluation

Examples and comparative examples having the composition shown in Table 1 were prepared and their stability was evaluated. Examples and Comparative Examples were stored in a container in which oxygen permeation through the side was restricted and stored at temperature conditions of 4°C, 25°C, and 40°C, respectively. Tea time) and 60 days (four time time), the color and the degree of suspension were visually observed and recorded in Table 7 below.

Detailed evaluation criteria are as follows. ○(no change), △(there is a very small amount of floating matter), X(there is floating matter and color change), ★(there is dark color change). In this case, the dark color change means a state in which the background surface of the sample is significantly covered by the suspension of the sample, and it means a state in which yellow color is clearly observed from the sample.

According to the evaluation, in the case of the comparative example, the occurrence of floating matter and color change were clearly observed. This is thought to be the result of excessive progression of the imine formation reaction (Mayard reaction) between the AFA extract and cytokines. On the other hand, when the results of Comparative Example 3 and Example 8 are summarized, it is determined that in the cosmetic composition for skin regeneration of the present invention, the addition of chrysine has a significant effect on rancidity and denaturation of the compound.

division Tea Example Comparative example One 2 5 8 One 2 3 4℃ One ○ ○ ○ ○ ○ ○ ○ 2 ○ ○ ○ ○ ○ ○ ○ 3 ○ ○ ○ ○ ○ △ ○ 4 ○ ○ ○ ○ △ △ △ 25℃ One ○ ○ ○ ○ ○ ○ ○ 2 ○ ○ ○ ○ △ △ ○ 3 ○ ○ ○ ○ △ △ △ 4 ○ ○ △ ○ △ X △ 40℃ One ○ ○ ○ ○ △ △ ○ 2 ○ ○ ○ ○ X ★ △ 3 ○ △ △ ○ X ★ △ 4 ○ △ △ △ ★ ★ X

Claims (6)

In the cosmetic composition for skin regeneration consisting of an aqueous layer containing a peptide compound as an active ingredient and an oil layer containing MCT,
The aqueous layer contains AFA extract, the oil layer contains flavonoids,
The peptide compounds include cytokines EGF (epidermal growth factor), AFGF (anti-aging growth factor), VEGF (vascular endothelial growth factor), FGF (fibroblast growth factor), IGF (insulin-like growth factor), KGF ( Keratinocyte growth factor) is mixed in a weight ratio of 12:1.5:1:1:1:1,
The AFA extract removes reducing saccharides by extracting AFA with 80% ethanol at 60°C for 3 hours,
The flavonoid is one or more selected from the group consisting of flavone compounds Chrysin, tectochrysin, primetin, apigenin, and acetin, a cosmetic composition for skin regeneration . delete The method of claim 1,
The oily layer further comprises one or more polyunsaturated fatty acids selected from the group consisting of omega-3, omega-6, and omega-9, a cosmetic composition for skin regeneration. delete delete delete