KR102126840B1 - method for producing petal of rose of Sharon extract, cosmetic composition for whitening skin comprising petal of rose of Sharon extract and pharmaceutical composition for prevention or treatment of melanin hyperpigmentation disease containing thereof - Google Patents
method for producing petal of rose of Sharon extract, cosmetic composition for whitening skin comprising petal of rose of Sharon extract and pharmaceutical composition for prevention or treatment of melanin hyperpigmentation disease containing thereof Download PDFInfo
- Publication number
- KR102126840B1 KR102126840B1 KR1020180079354A KR20180079354A KR102126840B1 KR 102126840 B1 KR102126840 B1 KR 102126840B1 KR 1020180079354 A KR1020180079354 A KR 1020180079354A KR 20180079354 A KR20180079354 A KR 20180079354A KR 102126840 B1 KR102126840 B1 KR 102126840B1
- Authority
- KR
- South Korea
- Prior art keywords
- green light
- extract
- melanin
- petal
- petal extract
- Prior art date
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Abstract
본 발명은 무궁화 꽃잎 추출물의 제조방법, 무궁화 꽃잎 추출물을 유효성분으로 포함하는 피부 미백용 화장료 조성물 및 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물에 관한 것으로써, 보다 상세하게는 멜라닌(melanin)의 생성을 현저히 억제하는 무궁화 꽃잎 추출물의 제조방법, 무궁화 꽃잎 추출물을 유효성분으로 포함하는 피부 미백용 화장료 조성물 및 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물에 관한 것이다.The present invention relates to a method for preparing a rose petal extract, a cosmetic composition for skin whitening comprising the rose petal petal extract as an active ingredient, and a pharmaceutical composition for preventing or improving melanin hyperpigmentation disease, and more specifically, melanin It relates to a method for preparing a green light petal extract that significantly suppresses the production, a cosmetic composition for skin whitening comprising the green light petal extract as an active ingredient, and a pharmaceutical composition for preventing or improving melanin hyperpigmentation disease.
Description
본 발명은 무궁화 꽃잎 추출물의 제조방법, 무궁화 꽃잎 추출물을 유효성분으로 포함하는 피부 미백용 화장료 조성물 및 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물에 관한 것으로써, 보다 상세하게는 멜라닌(melanin)의 생성을 현저히 억제하는 무궁화 꽃잎 추출물의 제조방법, 무궁화 꽃잎 추출물을 유효성분으로 포함하는 피부 미백용 화장료 조성물 및 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물에 관한 것이다.The present invention relates to a method for preparing a petal of green light, a cosmetic composition for skin whitening comprising the green light petal extract as an active ingredient, and a pharmaceutical composition for preventing or improving melanin hyperpigmentation disease, and more specifically, melanin. It relates to a method for preparing a green light petal extract that significantly suppresses the production, a cosmetic composition for skin whitening comprising the green light petal extract as an active ingredient, and a pharmaceutical composition for preventing or improving melanin hyperpigmentation disease.
멜라닌은 피부, 머리카락, 눈 그리고 다른 색소가 침착되는 조직에 존재하는 갈색 또는 흑색의 색소로, 사람의 외모에서 큰 비중을 차지하며, 피부 항상성을 유지하는 데에도 중요하다. 피부의 멜라닌 생합성은 호르몬 변화와 영양 상태 등 다양한 요인의 영향을 받으며, 멜라닌이 정상적으로 생성되지 않고 생합성 과정이 교란되면 색소 침착 감소 또는 과잉 색소 침착으로 분류되는 색소 침착의 결함이 발생하게 된다. 색소 침착의 결함은 유전적이거나 후천적일 수 있고, 일시적이거나 영구적일 수 있으며, 또한 신체 일부 또는 전체적으로 발생할 수 있다. 멜라닌이 비정상적으로 축적되거나 분포하게 되면 외관상 비호감을 줄 수 있는 기미, 주근깨, 노인성 흑점 등이 유발되기 때문에, 원치 않는 멜라닌의 축적을 방지하는 것은 화장품 업계의 초미의 관심사이다.Melanin is a brown or black pigment that is present in the skin, hair, eyes, and tissues where other pigments are deposited. It is a major part of a person's appearance and is also important for maintaining skin homeostasis. Melanin biosynthesis of the skin is influenced by various factors such as hormonal changes and nutritional status, and if melanin is not normally produced and the biosynthesis process is disturbed, pigmentation defects classified as reduced pigmentation or excessive pigmentation occur. Defects in pigmentation can be genetic or acquired, temporary or permanent, and can also occur part or all of the body. Preventing the accumulation of undesired melanin is a concern in the cosmetics industry because melanin accumulates or distributes abnormally, which may cause tingling, freckles, and senile black spots that may give an unfavorable appearance.
또한, 멜라닌의 생합성은 아미노산의 일종인 티로신(tyrosin)을 전구물질로 하여, 도파(DOPA), 도파 퀴논(DOPA quinone) 및 인돌-5,6-디히드로퀴논(indole-5,6-dihydroquinone)을 거쳐 인돌-5,6-디히드로퀴논의 중합체인 멜라닌으로 생합성 된다. 멜라닌은 멜라노사이트(melanocyte) 내에서 생성되며, 멜라노솜(melanosome)이라는 과립 형태로 표피와 진피의 경계부로 이동하여 침착된다. 멜라닌은 검은 피부의 직접적인 원인이 될 뿐만 아니라, 기미, 주근깨의 형성을 촉진하는 등 피부 미용 측면에서 심각한 문제를 일으킨다. 또한 과잉 생산된 멜라닌은 피부노화 및 피부암을 유발하는 원인이 되는 것으로 밝혀졌다.In addition, the biosynthesis of melanin is tyrosine, which is a kind of amino acid, as a precursor, and thus, dopa (DOPA), dopa quinone (DOPA quinone) and indole-5,6-dihydroquinone (indole-5,6-dihydroquinone) It is then biosynthesized into melanin, a polymer of indole-5,6-dihydroquinone. Melanin is produced in melanocytes and moves to the border between the epidermis and dermis in the form of granules called melanosomes and is deposited. Melanin is not only a direct cause of dark skin, but also causes serious problems in terms of skin beauty, such as promoting the formation of blemishes and freckles. In addition, it was found that the over-produced melanin causes skin aging and skin cancer.
한편, 우리나라의 국화인 무궁화(rose of Sharon, 학명 : Hibiscus syriacus)는 국내에서만 약 200여종이 있는 것으로 알려져 있으며, 목근피(Hibisci cortex)는 무궁화의 뿌리 및 줄기 껍질부분으로 예로부터 한방에서 소염, 항균 등의 효능을 가진 약용식물로 이용되어 왔다. 하지만, 무궁화가 우리나라의 국화임에도 불구하고 현재까지 국내에서는 무궁화의 재비 및 육종에 대한 연구만 주로 이루어지고 있으며, 무궁화를 이용한 다양한 생리활성 연구가 미흡하고, 특히 생활 속에 적용 가능한 화장료 조성물 또는 약학조성물로서 개발 가능한 연구 결과는 거의 없다.On the other hand, the national flower of Korea, rose of Sharon (scientific name: Hibiscus syriacus ), is known to have about 200 species only in Korea, and Hibisci cortex is the root and stem bark part of Mugunghwa. It has been used as a medicinal plant with antibacterial properties. However, despite the fact that Mugunghwa is the national flower of Korea, until now, only research on fertilization and breeding of Mugunghwa has been conducted in Korea, and various physiological activity studies using Mugunghwa are insufficient, especially as a cosmetic composition or pharmaceutical composition applicable to life. There are few research results that can be developed.
본 발명은 상기와 같은 점을 감안하여 안출한 것으로, 멜라닌(melanin)의 생성을 현저히 억제하여, 피부 미백 효과 또는 멜라닌 색소 과다 침착 질환의 예방 또는 개선 효과를 가지는 무궁화 꽃잎 추출물의 제조방법, 무궁화 꽃잎 추출물을 유효성분으로 포함하는 피부 미백용 화장료 조성물 및 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물을 제공하는데 목적이 있다.The present invention was devised in view of the above points, and it significantly suppresses the production of melanin, thereby producing a method of producing a light green petal extract having a skin whitening effect or a prevention or improvement effect of a melanin hyperpigmentation disease, light green petals It is an object of the present invention to provide a cosmetic composition for skin whitening comprising an extract as an active ingredient and a pharmaceutical composition for preventing or improving melanin hyperpigmentation disease.
상술한 과제를 해결하기 위하여, 본 발명의 무궁화 꽃잎 추출물의 제조방법은 무궁화 꽃잎을 준비하는 제1단계, 상기 무궁화 꽃잎에 C1 ~ C4의 알코올 용매를 첨가하고, 암조건에서 정치 추출하여 무궁화 꽃잎의 알코올 추출물을 수득하는 제2단계 및 상기 알코올 추출물을 감압 농축하여 무궁화 꽃잎 추출물을 제조하는 제3단계를 포함할 수 있다.In order to solve the above-mentioned problems, the method of preparing the green light petals of the present invention is the first step of preparing the green light petals, adding an alcohol solvent of C1 to C4 to the green light petals, and extracting the green light in the dark condition to extract the green light petals. The second step of obtaining an alcohol extract and the third step of preparing the green light petal extract by concentrating the alcohol extract under reduced pressure may be included.
본 발명의 바람직한 일실시예로서, 상기 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제할 수 있다.As a preferred embodiment of the present invention, the green light petal extract can inhibit the production of melanin.
본 발명의 바람직한 일실시예로서, 상기 무궁화 꽃잎 추출물은 피부 미백용 또는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용일 수 있다.As a preferred embodiment of the present invention, the green light petal extract may be used for skin whitening or prevention or improvement of melanin hyperpigmentation disease.
본 발명의 바람직한 일실시예로서, 상기 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래일 수 있다.In one embodiment of the present invention, the extract of rose of Sharon Hibiscus petals "back single fiber" may be derived from (scientific name:: Hibiscus syriacus' Paektanshim ') or Hibiscus "Phoenix" (Hibiscus syriacus' Pulsae "nomenclature).
한편, 본 발명의 피부 미백용 화장료 조성물은 무궁화 꽃잎 추출물을 유효성분으로 포함한다.On the other hand, the cosmetic composition for skin whitening of the present invention includes an extract of green light petals as an active ingredient.
본 발명의 바람직한 일실시예로서, 본 발명의 피부 미백용 화장료 조성물의 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제할 수 있다.As a preferred embodiment of the present invention, the green light petal extract of the cosmetic composition for skin whitening of the present invention can inhibit the production of melanin.
본 발명의 바람직한 일실시예로서, 본 발명의 피부 미백용 화장료 조성물의 무궁화 꽃잎 추출물은 무궁화 꽃잎을 암조건에서 정치 추출한 알코올 추출물일 수 있다.As a preferred embodiment of the present invention, the green light petal extract of the cosmetic composition for skin whitening of the present invention may be an alcohol extract obtained by statically extracting the green light petals under dark conditions.
본 발명의 바람직한 일실시예로서, 본 발명의 피부 미백용 화장료 조성물은 무궁화 꽃잎 추출물을 100 ~ 600ug/ml의 농도로 포함할 수 있다.As a preferred embodiment of the present invention, the cosmetic composition for skin whitening of the present invention may include a green light petal extract at a concentration of 100 ~ 600ug / ml.
나아가, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물은 무궁화 꽃잎 추출물을 유효성분으로 포함한다.Furthermore, the pharmaceutical composition for the prevention or improvement of melanin hyperpigmentation disease of the present invention includes a green light petal extract as an active ingredient.
본 발명의 바람직한 일실시예로서, 상기 멜라닌 색소 과다 침착 질환은 주근깨, 노인성 반점, 간반, 기미, 흑점, 일광색소반, 푸른흑피증(cyanic melasma), 약물 사용 후의 과다색소침착, 임신성 갈색반(gravidic chloasma), 또는 상처 또는 피부염으로 인한 염증 후 과다 색소 침착일 수 있다.As a preferred embodiment of the present invention, the melanin hyperpigmentation diseases include freckles, senile plaques, liver spots, spots, sunspots, sun pigmentation, blue melasma, hyperpigmentation after drug use, gestational brown spots ( gravidic chloasma), or hyperpigmentation after inflammation due to wounds or dermatitis.
본 발명의 바람직한 일실시예로서, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물의 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제할 수 있다.As a preferred embodiment of the present invention, the green light petal extract of the pharmaceutical composition for preventing or improving melanin hyperpigmentation disease of the present invention can inhibit the production of melanin.
본 발명의 바람직한 일실시예로서, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물의 무궁화 꽃잎 추출물은 무궁화 꽃잎을 암조건에서 정치 추출한 알코올 추출물일 수 있다.As a preferred embodiment of the present invention, the green light petal extract of the pharmaceutical composition for preventing or improving melanin hyperpigmentation disease of the present invention may be an alcohol extract obtained by statically extracting green light petals under dark conditions.
본 발명의 바람직한 일실시예로서, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물은 무궁화 꽃잎 추출물을 100 ~ 600ug/ml의 농도로 포함할 수 있다.As a preferred embodiment of the present invention, the pharmaceutical composition for the prevention or improvement of melanin hyperpigmentation disease of the present invention may include a green light petal extract at a concentration of 100 ~ 600ug/ml.
한편, 본 발명의 피부 미백용 건강기능성 식품은 무궁화 꽃잎 추출물을 유효성분으로 포함한다.On the other hand, the health functional food for skin whitening of the present invention includes a green light petal extract as an active ingredient.
나아가, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 건강기능성 식품은 무궁화 꽃잎 추출물을 유효성분으로 포함한다.Furthermore, the health functional food for prevention or improvement of melanin hyperpigmentation disease of the present invention includes a green light petal extract as an active ingredient.
본 발명의 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제하는 효과가 우수하다.The green light petal extract of the present invention is excellent in suppressing the production of melanin.
또한, 본 발명의 무궁화 꽃잎 추출물은 피부 미백 효과가 우수하다.In addition, the green light petal extract of the present invention is excellent in skin whitening effect.
또한, 본 발명의 무궁화 꽃잎 추출물은 멜라닌 색소 과다 침착 질환의 예방 또는 개선 효과가 우수하다.In addition, the green light petal extract of the present invention is excellent in preventing or improving melanin pigment hyperpigmentation disease.
도 1은 실시예 1과 2에서 제조된 무궁화{불새(PS)와 백단심(PTS)} 꽃잎 추출물 처리에 따른 세포내의 상대적 멜라닌(Melanin) 생성량을 나타낸 그래프이다.
도 2는 실시예 1과 2에서 제조된 무궁화{불새(PS)와 백단심(PTS)} 꽃잎 추출물 처리에 따른 세포외로 방출되는 상대적 멜라닌(Melanin) 생성량을 나타낸 그래프이다.
도 3은 실시예 1에서 제조된 무궁화 꽃잎 추출물(백단심-PTS) 처리에 따른 입체 현미경하에서 제브라피쉬의 멜라닌 합성을 측정한 사진이다.
도 4는 실시예 2에서 제조된 무궁화 꽃잎 추출물(불새-PS) 처리에 따른 입체 현미경하에서 제브라피쉬의 멜라닌 합성을 측정한 사진이다.1 is a graph showing the relative amount of melanin produced in cells according to the petal extract treatment of Mugunghwa (firebird (PS) and sandalwood core (PTS)) prepared in Examples 1 and 2.
Figure 2 is a graph showing the relative amount of melanin produced relative to the extracellular discharge according to the petal extract treatment prepared in Example 1 and 2 of Mugunghwa {firebird (PS) and sandalwood core (PTS)}.
FIG. 3 is a photograph of measuring the melanin synthesis of zebrafish under a stereoscopic microscope according to the treatment of green light petal extract (sandalwood core-PTS) prepared in Example 1.
Figure 4 is a photograph of measuring the melanin synthesis of zebrafish under a stereoscopic microscope according to the green light petal extract (firebird-PS) treatment prepared in Example 2.
이하, 첨부한 도면을 참고로 하여 본 발명의 실시예에 대하여 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 상세히 설명한다. 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다. 도면에서 본 발명을 명확하게 설명하기 위해서 설명과 관계없는 부분은 생략하였으며, 명세서 전체를 통하여 동일 또는 유사한 구성요소에 대해서는 동일한 참조부호를 부가한다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present invention pertains can easily practice. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. In the drawings, parts not related to the description are omitted in order to clearly describe the present invention, and the same reference numerals are added to the same or similar elements throughout the specification.
본 발명의 무궁화 꽃잎 추출물의 제조방법은 하기 제1단계 내지 제3단계를 포함한다.The method for preparing the petals of the green light of the present invention includes the following first steps to third steps.
먼저, 무궁화 꽃잎 추출물의 제조방법의 제1단계는 무궁화 꽃잎을 준비할 수 있다.First, in the first step of the method for preparing the petals of green light, the petals of green light can be prepared.
무궁화(rose of Sharon)는 쌍떡잎식물 아욱목 아욱과의 낙엽관목으로서, 무궁화 품종으로서 대략 200여 종의 품종이 있고, 품종마다 고유의 특징이 있다. 본 발명의 무궁화 꽃잎 추출물의 제조방법에서 사용할 수 있는 무궁화는 백단심계 홑꽃 무궁화'백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 홍단심계 홑꽃 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae')일 수 있다.The Rose of Sharon is a deciduous shrub of the dicotyledonous tree, the mallow family, and there are about 200 varieties of the Mugunghwa variety, each with its own characteristics. Mugunghwa that can be used in the method of preparing the petals of the green light of the present invention is a sandalwood 홑 flower ``flower sandalwood'' (Scientific name: Hibiscus syriacus'Paektanshim ') or a red heart system 홑flower green light'firebird' (Scientific name: Hibiscus syriacus'Pulsae ') Can.
무궁화 꽃잎 추출물의 제조방법의 제1단계는 무궁화의 줄기와 뿌리를 제거한 무궁화 꽃만을 준비하고, 무궁화 꽃에서도 꽃술과 씨방을 제거한 무궁화 꽃잎만을 준비할 수 있다.In the first step of the method for preparing the petals of green light, only green light flowers with stems and roots of green light can be prepared, and only green light petals without flowers and seeds are removed from green flowers.
다음으로, 무궁화 꽃잎 추출물의 제조방법의 제2단계는 제1단계에서 준비한 무궁화 꽃잎에 C1 ~ C4의 알코올 용매, 바람직하게는 에탄올 용매를 첨가하고, 암조건에서 정치 추출하여 무궁화 꽃잎의 알코올 추출물을 수득할 수 있다.Next, in the second step of the method for preparing the petals of green light, an alcohol solvent of C1 to C4, preferably an ethanol solvent, is added to the green light petals prepared in the first step, and the alcoholic extract of green light is extracted by static extraction under dark conditions. Can be obtained.
구체적으로, 무궁화 꽃잎 추출물의 제조방법의 제2단계는 제1단계에서 준비하 무궁화 꽃잎을 분쇄하고, 분쇄한 무궁화 꽃잎에 에탄올 용매를 첨가한 후 교반할 수 있으며, 교반 후에는 15 ~ 35℃의 온도, 바람직하게는 20 ~ 30℃의 온도에서 1 ~ 5일, 바람직하게는 2 ~ 4일간 암조건에서 정치 추출하여 무궁화 꽃잎의 알코올 추출물을 수득할 수 있다. 한편, 정치 추출을 암조건에서 수행하는 이유는 무궁화 꽃잎에 함유되어 있는 성분 중 하나인 안토시아닌 때문이며, 만일 암조건이 아닌 명조건에서 정치 추출을 수행하면 안토시아닌의 화학적 성분이 변화되는 문제가 발생할 수 있다.Specifically, the second step of the method for preparing the petals of green light can be prepared in the first step, crush the green light petals, add ethanol solvent to the crushed green light petals and stir, and after stirring, Alcohol extraction of the petals of green light can be obtained by static extraction under dark conditions for 1 to 5 days, preferably 2 to 4 days at a temperature, preferably 20 to 30°C. On the other hand, the reason for performing the political extraction in the dark condition is due to anthocyanin, which is one of the components contained in the petals of the green light, and if the political extraction is performed in the bright condition other than the dark condition, the chemical component of the anthocyanin may change. .
또한, 무궁화 꽃잎 추출물의 제조방법의 제2단계의 정치 추출물은 1 ~ 5회, 바람직하게는 2 ~ 4회 반복적으로 수행할 수 있다.In addition, the stationary extract of the second step of the method for preparing the petals of green light can be repeatedly performed 1-5 times, preferably 2-4 times.
마지막으로, 무궁화 꽃잎 추출물의 제조방법의 제3단계는 제2단계에서 수득한 알코올 추출물을 감압 농축하여 무궁화 꽃잎 추출물을 제조할 수 있다. 감압 농축은 10 ~ 50℃의 온도, 바람직하게는 20 ~ 40℃의 온도에서 감압 농축기를 이용하여 수행할 수 있다. 또한, 제조한 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래일 수 있다.Finally, the third step of the method for preparing the petals of green light can be prepared by extracting the petals of green light by concentrating the alcohol extract obtained in the second step under reduced pressure. Concentration under reduced pressure may be performed using a reduced pressure concentrator at a temperature of 10 to 50°C, preferably 20 to 40°C. Furthermore, the manufacturing Hibiscus petal extracts of Hibiscus 'back single-core' can be derived from (scientific name:: Hibiscus syriacus 'Paektanshim') or Sharon 'The Firebird' (Hibiscus syriacus 'Pulsae' scientific name).
한편, 본 발명의 무궁화 꽃잎 추출물의 제조방법으로 제조한 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제할 수 있다.Meanwhile, the green light petal extract prepared by the method of preparing the green light petal extract of the present invention can suppress the production of melanin.
나아가, 본 발명의 무궁화 꽃잎 추출물의 제조방법으로 제조된 무궁화 꽃잎 추출물은 피부 미백용 또는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용일 수 있다.Furthermore, the green light petal extract prepared by the method of preparing the green light petal extract of the present invention may be used for skin whitening or prevention or improvement of melanin hyperpigmentation disease.
한편, 본 발명은 피부 미백용 화장료 조성물은 무궁화 꽃잎 추출물을 유효성분을 포함한다. 이 때, 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제할 수 있다. 또한, 무궁화 꽃잎 추출물은 무궁화 꽃잎을 암조건에서 정치 추출한 알코올 추출물일 수 있다. 또한, 무궁화 꽃일 추출물을 100 ~ 600ug/ml의 농도, 바람직하게는 300 ~ 500ug/ml의 농도로 포함하여 최적의 멜라닌(melanin) 생성 억제 효과를 가질 수 있다.On the other hand, in the present invention, the cosmetic composition for skin whitening includes an active ingredient in the petals of green light petals. At this time, the green light petal extract can inhibit the production of melanin. In addition, the green light petal extract may be an alcohol extract obtained by statically extracting the green light petals under dark conditions. In addition, it can have an optimal melanin production inhibitory effect by including the flower of Hibiscus flower extract in a concentration of 100 ~ 600ug / ml, preferably 300 ~ 500ug / ml.
또한, 본 발명의 피부 미백용 화장료 조성물이 가지는 미백 효과란, 자외선 노출, 호르몬 밸런스의 변화, 유전적 프로그램 등의 각종 요인에 의해 발생하는 거뭇한 피부나 기미, 주근깨, 다크서클을 개선 또는 방지하는 효과, 피부를 투명감 있는 아름다운 것으로 하는, 혹은 투명감 있는 아름다운 피부를 유지하는 효과, 피부의 칙칙함을 경감하여 윤기ㆍ팽팽함을 증가시키는 효과 등을 말할 수 있다. 일반적으로, 거뭇한 피부나 기미, 주근깨, 다크서클은 자외선에 의한 자극이나 호르몬 밸런스의 변화 등에 의해 멜라노사이트(melanocyte)가 자극되고, 그래서 생합성된 멜라닌 색소가 피부에 침착하여 발생한다고 알려져 있다. 따라서, 멜라닌의 생성을 억제할 수 있다면, 거뭇한 피부나 기미, 주근깨, 다크서클을 예방ㆍ개선하는 것이 가능하다.In addition, the whitening effect of the cosmetic composition for skin whitening according to the present invention is to improve or prevent dark skin, blemishes, freckles, and dark circles caused by various factors such as ultraviolet exposure, changes in hormone balance, and genetic programs. The effect, the effect of making the skin transparent and beautiful, or the effect of maintaining the transparent and beautiful skin, the effect of reducing the dullness of the skin and increasing the shine and tautness can be mentioned. In general, it is known that melanocytes are stimulated by dark skin, blemishes, freckles, and dark circles due to stimulation by ultraviolet rays or changes in hormone balance, so that the biosynthesized melanin pigment is deposited on the skin. Therefore, if melanin production can be suppressed, it is possible to prevent and improve dark skin, blemishes, freckles, and dark circles.
한편, 본 발명의 화장료 조성물은 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 이루어지는 군으로부터 선택된 어느 하나의 제형을 가질 수 있으나, 이에 제한되지 않는다. 이들 각 제형으로 이루어진 화장료 조성물은 그 제형의 제제화에 필요하고 적절한 각종의 기제와 첨가물을 함유할 수 있으며, 이들 성분의 종류와 양은 당업자에 의해 용이하게 선정될 수 있다.On the other hand, the cosmetic composition of the present invention is from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays It may have any one formulation selected, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.
본 발명의 화장료 조성물은 유효성분 이외에 추가로 동일 또는 유사한 기능을 나타내는 피부 미백 활성 성분을 1종 이상 함유할 수 있다. 피부 미백 활성 성분으로는 코지산 및 이의 유도체, 알부틴, 아스코르브산 및 이의 유도체, 하이드로퀴논 및 이의 유도체, 레조르시놀, 사이클로알카논, 메틸렌디옥시페닐 알칸올, 2,7-디니트로인다졸 또는 덩굴귤 추출물, 쌀 추출물, 감초 추출물과 같은 식물 추출물 등이 있으나, 이에 제한되는 것은 아니다.The cosmetic composition of the present invention may contain one or more skin whitening active ingredients exhibiting the same or similar function in addition to the active ingredient. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenyl alkanol, 2,7-dinitroindazole or There are vine extracts, rice extracts, plant extracts such as licorice extract, but are not limited thereto.
본 발명의 화장료 조성물의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유, 식물섬유, 왁스, 파라핀, 전분, 트라가칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components Etc. can be used.
본 발명의 화장료 조성물의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판-부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally, chlorofluorohydro Propellants such as carbon, propane-butane or dimethyl ether.
본 발명의 화장료 조성물의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매, 용매화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene Glycols, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.
본 발명의 화장료 조성물의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라칸트 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.
본 발명의 화장료 조성물의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르설페이트, 설포숙신산 모노에스테르, 아세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 리놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate as a carrier component , Fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives or ethoxylated glycerol fatty acid esters and the like.
본 발명의 화장료 조성물은 형광물질, 살진균제, 굴수성 유발물질, 보습제, 방향제, 방향제 담체, 단백질, 용해화제, 당 유도체, 일광차단제, 비타민, 식물 추출물 등을 포함하는 부형제를 추가로 함유할 수 있다.The cosmetic composition of the present invention may further contain excipients including fluorescent materials, fungicides, oleogenic agents, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, etc. .
나아가, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물은 무궁화 꽃잎 추출물을 유효성분을 포함한다. 이 때, 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제할 수 있다. 또한, 무궁화 꽃잎 추출물은 무궁화 꽃잎을 암조건에서 정치 추출한 알코올 추출물일 수 있다. 또한, 무궁화 꽃잎 추출물을 100 ~ 600ug/ml의 농도, 바람직하게는 300 ~ 500ug/ml의 농도로 포함하여 최적의 멜라닌(melanin) 생성 억제 효과를 가질 수 있다.Furthermore, the pharmaceutical composition for the prevention or improvement of melanin hyperpigmentation disease of the present invention includes an active ingredient in the petals of green light. At this time, the green light petal extract can inhibit the production of melanin. In addition, the green light petal extract may be an alcohol extract obtained by statically extracting the green light petals under dark conditions. In addition, it can have an optimal melanin production inhibitory effect by including the green light petal extract in a concentration of 100 ~ 600ug / ml, preferably 300 ~ 500ug / ml.
또한, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물은 무궁화 꽃잎 추출물 외에 담체, 부형제 또는 희석제를 더 포함할 수 있지만, 이에 한정하는 것은 아니다.In addition, the pharmaceutical composition for preventing or improving melanin hyperpigmentation disease of the present invention may further include a carrier, excipient, or diluent in addition to the green light petal extract, but is not limited thereto.
본 발명에서 사용되는 용어인 "멜라닌 색소 과다 침착(hyperpigmentation)"은 피부 또는 손·발톱의 특정 부위에서 멜라닌의 과도한 증가에 의해 다른 부위에 비해 검게 또는 어둡게 되는 것을 의미한다.The term "hyperpigmentation of melanin", which is used in the present invention, means that it becomes darker or darker than other sites due to excessive increase in melanin in a specific part of the skin or nails or toenails.
또한, 본 발명의 멜라닌 색소 과다침착 질환은 주근깨, 노인성 반점, 간반, 기미, 흑점, 일광 색소반, 푸른흑피증(cyanic melasma), 약물 사용 후의 과다색소침착, 임신성 갈색반(gravidic chloasma), 또는 상처 또는 피부염으로 인한 염증 후 과다 색소 침착 등을 포함하나 이에 한정되지 않는다.In addition, the melanin hyperpigmentation disease of the present invention may include freckles, senile plaques, liver spots, spots, black spots, sun pigmented plaques, cyanic melasma, hyperpigmentation after drug use, and gestational chloasma, or And hyperpigmentation after inflammation due to wounds or dermatitis.
본 발명의 약학조성물은 경구 또는 비경구의 여러 가지 제형일 수 있다. 제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다. 경구투여를 위한 고형 제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형 제제는 하나 이상의 화합물에 적어도 하나 이상의 부형제 예를 들면, 전분, 탄산칼슘, 수크로오스(sucrose) 또는 락토오스(lactose), 젤라틴 등을 섞어 조제된다. 또한, 단순한 부형제 이외에 스테아린산 마그네슘, 탈크 등과 같은 윤활제들도 사용된다. 경구 투여를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당되는데 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구 투여를 위한 제제에는 멸균된 수용액, 비수성 용제, 현탁제, 유제, 동결건조제제, 좌제가 포함된다. 비수성 용제 및 현탁 용제로는 프로필렌글리콜(propylene glycol), 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔(witepsol), 마크로골, 트윈tween) 61, 카카오지, 라우린지, 글리세로 젤라틴 등이 사용될 수 있다.The pharmaceutical composition of the present invention may be various oral or parenteral formulations. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc.These solid preparations include at least one excipient in one or more compounds such as starch, calcium carbonate, sucrose or lactose ( lactose) and gelatin. In addition, lubricants such as magnesium stearate, talc and the like are used in addition to simple excipients. Liquid preparations for oral administration include suspending agents, intravenous solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used as diluents, various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, can be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, twin tween 61, cacao butter, laurin butter, and glycero gelatin may be used.
본 발명에 따른 약학조성물은 약제학적으로 유효한 양으로 투여한다. 본 발명에 있어서, "약제학적으로 유효한 양"은 의학적 치료에 적용 가능한 합리적인 수혜/위험 비율로 질환을 치료하기에 충분한 양을 의미하며, 유효용량 수준은 환자의 질환의 종류, 중증도, 약물의 활성, 약물에 대한 민감도, 투여 시간, 투여 경로 및 배출 비율, 치료기간, 동시 사용되는 약물을 포함한 요소 및 기타 의학 분야에 잘 알려진 요소에 따라 결정될 수 있다. 본 발명의 약학조성물은 개별 치료제로 투여하거나 다른 치료제와 병용하여 투여될 수 있고 종래의 치료제와는 순차적 또는 동시에 투여될 수 있으며, 단일 또는 다중 투여될 수 있다. 상기한 요소들을 모두 고려하여 부작용없이 최소한의 양으로 최대 효과를 얻을 수 있는 양을 투여하는 것이 중요하며, 이는 당업자에 의해 용이하게 결정될 수 있다.The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "a pharmaceutically effective amount" means an amount sufficient to treat the disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of patient's disease, severity, and activity of the drug , The sensitivity to the drug, the time of administration, the route of administration and rate of excretion, the duration of treatment, factors including the drugs used simultaneously, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered in single or multiple doses. Considering all of the above factors, it is important to administer an amount that can achieve the maximum effect in a minimal amount without side effects, which can be easily determined by those skilled in the art.
본 발명의 약학조성물의 투여량은 환자의 체중, 연령, 성별, 건강상태, 식이, 투여시간, 투여방법, 배설률 및 질환의 중증도에 따라 그 범위가 다양하게 사용할 수 있다.The dosage of the pharmaceutical composition of the present invention can be variously used according to the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate and disease severity.
한편, 본 발명의 피부 미백용 건강기능성 식품은 무궁화 꽃잎 추출물을 유효성분으로 포함한다. 또한, 본 발명의 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 건강기능성 식품은 무궁화 꽃잎 추출물을 유효성분으로 포함한다.On the other hand, the health functional food for skin whitening of the present invention includes a green light petal extract as an active ingredient. In addition, the health functional food for prevention or improvement of melanin hyperpigmentation disease of the present invention includes a green light petal extract as an active ingredient.
본 발명의 건강기능성 식품을 식품첨가물로 사용하는 경우, 상기 건강기능성 식품을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분의 양은 그의 사용 목적(예방 또는 개선)에 따라 적절하게 사용될 수 있다. 일반적으로, 식품 또는 음료의 제조시 본 발명의 건강기능성 식품은 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가된다. 그러나 건강을 목적으로 하는 장기간의 섭취한 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로 사용될 수 있다.When the health functional food of the present invention is used as a food additive, the health functional food may be added as it is or used with other foods or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient may be appropriately used depending on the purpose of use (prevention or improvement). Generally, the health functional food of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, with respect to the raw materials in the manufacture of a food or beverage. However, when ingested for a long period of time for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.
또한, 건강기능성 식품의 종류에 특별한 제한은 없다. 건강기능성 식품을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강 식품을 모두 포함한다.In addition, there are no particular restrictions on the type of health functional food. Examples of foods to which health functional foods can be added are meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, dairy products including gums, ice cream, various soups, beverages, tea drinks, Alcoholic beverages, vitamin complexes, and the like, and include all healthy foods in the ordinary sense.
또한, 본 발명의 건강기능성 식품은 식품, 특히 기능성 식품으로 제조될 수 있다. 본 발명의 기능성 식품은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함한다. 예컨대, 드링크제로 제조되는 경우에는 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함시킬 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로오스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등) 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐 등)를 이용할 수 있다.In addition, the health functional food of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention includes ingredients that are conventionally added during food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink agent, natural carbohydrates or flavoring agents may be included as additional components in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg. , Xylitol, sorbitol, erythritol, and the like). The flavoring agent may be a natural flavoring agent (for example, taumatin, stevia extract, etc.) and a synthetic flavoring agent (for example, saccharin, aspartame, etc.).
또한, 건강기능성 식품 이외에 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다.In addition, in addition to health functional food, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonizing agent used in beverages.
이상에서 본 발명에 대하여 구현예를 중심으로 설명하였으나 이는 단지 예시일 뿐 본 발명의 구현예를 한정하는 것이 아니며, 본 발명의 실시예가 속하는 분야의 통상의 지식을 가진 자라면 본 발명의 본질적인 특성을 벗어나지 않는 범위에서 이상에 예시되지 않은 여러 가지의 변형과 응용이 가능함을 알 수 있을 것이다. 예를 들어, 본 발명의 구현예에 구체적으로 나타난 각 구성 요소는 변형하여 실시할 수 있는 것이다. 그리고 이러한 변형과 응용에 관계된 차이점들은 첨부된 청구 범위에서 규정하는 본 발명의 범위에 포함되는 것으로 해석되어야 할 것이다.In the above, the present invention has been mainly described with reference to embodiments, but this is merely an example and does not limit the embodiments of the present invention, and those having ordinary knowledge in the field to which the embodiments of the present invention pertain will provide essential characteristics of the present invention. It will be appreciated that various modifications and applications not illustrated above are possible without departing from the scope. For example, each component specifically shown in the embodiment of the present invention can be implemented by modification. And differences related to these modifications and applications should be construed as being included in the scope of the invention defined in the appended claims.
준비예 1 : 시료 준비Preparation Example 1: Sample preparation
국내에서 육성된 백단심계 홑꽃인 무궁화 '백단심’ (학명 : Hibiscus syriacus 'Paektanshim')과 홍단심계 홑꽃인 무궁화 '불새’ (학명 : Hibiscus syriacus 'Pulsae')을 각각 준비하였다. 무궁화 '백단심’과 무궁화 '불새’는 국립산림과학원 산림생명자원연구부 무궁화 유전자원 보존원(경기 수원 소재)에 식재된 5 ~ 6년생 삽목묘 중 품종별 각각 3 ~ 5본을 대상으로 2017년 7월초 ~ 8월말 사이에 매일 아침 개화한 무궁화 꽃을 수집, 꽃술과 씨방을 제거한 무궁화 꽃잎만 모아 실험에 사용될 때까지 -80℃에 저장하여 시료로 준비하였다.Mugunghwa'sandalwood' (Scientific name: Hibiscus syriacus'Paektanshim '), a red sandalwood 심 flower, and Higunghwa'Firebird' (Scientific name: Hibiscus syriacus'Pulsae '), respectively, were developed in Korea. Mugunghwa'sandalwood' and Mugunghwa'firebird' are targeted at 3 to 5 of each 5 to 6-year-old shovel seedlings planted at the Mugunghwa Genetic Conservation Center (located in Suwon, Gyeonggi-do), Department of Forestry and Life Sciences, National Forest Science Institute. From the beginning of the month to the end of August, the flowers of Mugunghwa, which bloomed every morning, were collected, and only the petals of Higunghwa, which had been removed from the pistil and ovary, were collected and stored at -80°C until they were used in the experiment, and prepared as samples.
실시예 1 : 무궁화 꽃잎 추출물의 제조Example 1: Preparation of green light petal extract
준비된 시료 중 무궁화 '백단심’ (학명 : Hibiscus syriacus 'Paektanshim')의 무궁화 꽃잎을 믹서기를 이용하여 분쇄하였다. 분쇄된 무궁화 꽃잎에 30L의 에탄올을 첨가 및 교반하였다. 교반 후, 상온(25℃)에서 3일간 암조건에서 정치 추출하여 무궁화 꽃잎의 알코올 추출물을 수득하였다.Among the prepared samples, the green light petals of the green light'sandalwood core' (scientific name: Hibiscus syriacus'Paektanshim ') were pulverized using a blender. 30 L of ethanol was added and stirred to the pulverized green light petals. After stirring, the mixture was statically extracted under dark conditions at room temperature (25° C.) for 3 days to obtain an alcoholic extract of green light petals.
수득한 알코올 추출물을 3회 반복하여 추출하고, 추출된 추출물을 30℃에서 증기 감압 농축기를 이용하여 1L로 농축한 후, -70℃에서 하루동안 동결 건조하여 무궁화 꽃잎 추출물(PTS)을 제조하였다.The obtained alcohol extract was repeatedly extracted three times, and the extracted extract was concentrated to 1 L using a steam vacuum concentrator at 30° C., and then freeze-dried at -70° C. for one day to prepare a green light petal extract (PTS).
실시예 2 : 무궁화 꽃잎 추출물의 제조Example 2: Preparation of green light petal extract
준비된 시료 중 무궁화 '불새’ (학명 : Hibiscus syriacus 'Pulsae')의 무궁화 꽃잎을 믹서기를 이용하여 분쇄하였다. 분쇄된 무궁화 꽃잎에 30L의 에탄올을 첨가 및 교반하였다. 교반 후, 상온(25℃)에서 3일간 암조건에서 정치 추출하여 무궁화 꽃잎의 알코올 추출물을 수득하였다.Among the prepared samples, the green light petals of the green light'firebird' (scientific name: Hibiscus syriacus'Pulsae ') were pulverized using a blender. 30 L of ethanol was added and stirred to the pulverized green light petals. After stirring, the mixture was statically extracted under dark conditions at room temperature (25° C.) for 3 days to obtain an alcoholic extract of green light petals.
수득한 알코올 추출물을 3회 반복하여 추출하고, 추출된 추출물을 30℃에서 증기 감압 농축기를 이용하여 1L로 농축한 후, -70℃에서 하루동안 동결 건조하여 무궁화 꽃잎 추출물(PS)을 제조하였다.The obtained alcohol extract was repeatedly extracted 3 times, and the extracted extract was concentrated to 1 L using a steam vacuum concentrator at 30° C., and then freeze-dried at -70° C. for 1 day to prepare a green light petal extract (PS).
실험예 1 : 무궁화 꽃잎 추출물 처리에 따른 멜라닌(melanin)의 생성 억제 효과Experimental Example 1: Inhibition effect of melanin production according to the petal extract treatment
1. 세포내외 멜라닌 생합성 저해 효과 실험1. In vitro and intracellular melanin biosynthesis inhibitory effect experiment
멜라닌(Melanin) 생성량 측정은 Hosoi et al. (1985)의 방법을 변형하여 사용하였다. 배양한 B16F10 세포를 24-웰-플레이트(24 well plate)에 각각 1×105 cells/well로 접종(seeding)하고 24시간 배양하였다. 각 well에 1ug/ml의 α-멜라노사이트 자극 호르몬(α-melanocyte-stimulating hormone, α-MSH)를 처리하여 24시간동안 멜라닌 생성을 유도하고, 실시예 1에서 제조된 무궁화 꽃잎 추출물(PTS) 및 실시예 2에서 제조된 무궁화 꽃잎 추출물(PS)을 각각 0ug/ml, 25ug/ml, 100ug/ml, 400ug/ml의 농도로 처리한 후 72시간동안 배양하였다.Measurement of melanin production was determined by Hosoi et al. (1985) was used as a modification. The cultured B16F10 cells were seeded in 24-well-plates (24 well plates) at 1×10 5 cells/well, respectively, and cultured for 24 hours. Each well was treated with 1ug/ml of α-melanocyte-stimulating hormone (α-MSH) to induce melanin production for 24 hours, and the green light petal extract (PTS) prepared in Example 1 and The green light petal extract (PS) prepared in Example 2 was treated with concentrations of 0 ug/ml, 25 ug/ml, 100 ug/ml, and 400 ug/ml, respectively, and then cultured for 72 hours.
72시간동안 배양한 후 각 well을 PBS로 세척하였다. 세척 후 0.2N NaOH 용액 400 ㎕를 첨가하고 60℃에서 1 시간동안 용해시킨 후, micro plate reader로 405nm에서 흡광도를 측정하여, 세포내(intracellular)와 세포외(extracellular)에 존재하는 멜라닌 양을 측정하였다.After incubation for 72 hours, each well was washed with PBS. After washing, add 400 μl of 0.2N NaOH solution and dissolve at 60° C. for 1 hour, measure the absorbance at 405 nm with a micro plate reader, and measure the amount of melanin present in intracellular and extracellular. Did.
도 1은 실시예 1과 2에서 제조된 무궁화 꽃잎 추출물(PTS와 PS) 처리에 따른 세포 내(intracellular)에 함유하고 있는 상대적 멜라닌(Melanin) 생성량을 나타낸 그래프로서, 실시예 1에서 제조된 무궁화 꽃잎 추출물(PTS)을 400ug/ml의 농도로 처리할 때, α-MSH의 처리에 의해서 증가된 세포내 멜라닌합성을 31%, 100ug/ml의 농도로 처리할 때는 22%를, 25ug/ml의 농도로 처리할 때는 8%를 억제함을 확인 할 수 있었다. 실시예 2에서 제조된 무궁화 꽃잎 추출물(PS)을 400ug/ml의 농도로 처리할 때, α-MSH의 처리에 의해서 증가된 세포내 멜라닌합성을 29%, 100ug/ml의 농도로 처리할 때는 21%를, 25ug/ml의 농도로 처리할 때는 11%를 저해함을 확인 할 수 있었다.1 is a graph showing the relative amount of melanin produced in intracellular according to the treatment of green light petal extracts (PTS and PS) prepared in Examples 1 and 2, and light green petals prepared in Example 1 When the extract (PTS) was treated at a concentration of 400 ug/ml, the intracellular melanin synthesis increased by the treatment of α-MSH was 31%, and when treated at a concentration of 100 ug/ml, 22%, and a concentration of 25 ug/ml It was confirmed that 8% was suppressed when treated with. When the green light petal extract (PS) prepared in Example 2 was treated at a concentration of 400 ug/ml, when the intracellular melanin synthesis increased by the treatment of α-MSH was treated at a concentration of 29% and 100 ug/ml, 21 %, it was confirmed that inhibiting 11% when treated at a concentration of 25ug / ml.
도 2는 실시예 1과 2에서 제조된 무궁화 꽃잎 추출물(PTS 와 PS) 처리에 따른 세포외(extracellular)로 방출되는 상대적 멜라닌(Melanin) 생성량을 나타낸 그래프로서, 실시예 1에서 제조된 무궁화 꽃잎 추출물(PTS)을 400ug/ml의 농도로 처리할 때, α-MSH의 처리에 의해서 증가된 세포외 방출 멜라닌합성을 37%, 100ug/ml의 농도로 처리할 때는 31%를, 25ug/ml의 농도로 처리할 때는 16%를 억제함을 확인 할 수 있었다. 실시예 2에서 제조된 무궁화 꽃잎 추출물(PS)을 400ug/ml의 농도로 처리할 때, α-MSH의 처리에 의해서 증가된 세포외 방출 멜라닌합성을 36%, 100ug/ml의 농도로 처리할 때는 29%를, 25ug/ml의 농도로 처리할 때는 16%를 저해함을 확인 할 수 있었다.Figure 2 is a graph showing the relative amount of melanin (Melanin) produced by the extracellular (Extracellular) according to the green light petal extract (PTS and PS) treatment prepared in Examples 1 and 2, the green light petal extract prepared in Example 1 When (PTS) was treated at a concentration of 400 ug/ml, the concentration of extracellular release melanin increased by treatment of α-MSH was 37%, 31% when treated at a concentration of 100 ug/ml, and a concentration of 25 ug/ml It was confirmed that 16% was suppressed when treated with. When treating the green light petal extract (PS) prepared in Example 2 at a concentration of 400 ug/ml, when treating the increased extracellular release melanin synthesis by the treatment of α-MSH at a concentration of 36%, 100 ug/ml When treating 29% at a concentration of 25 ug/ml, it was confirmed that 16% was inhibited.
2. 제브라피쉬를 이용한 멜라닌 합성 저해 효과 실험2. Experiment of inhibitory effect of melanin synthesis using zebrafish
성숙한 제프라피쉬(zebrafish) 암수를 알 채취 전날 알 채취용 수조에 넣고, 다음날 광주기 시기 1 ~ 2시간 이후에 알을 채취하였다. 채취된 알은 zebrafish embryo medium에 넣고 48시간동안 발생시킨 후, 제프라피쉬의 배아를 회수하여 파이펫을 이용해 24-웰 플레이트의 각각에 배아를 분배하였다. 각 well에 투명한 제프라피쉬를 형성하기 위하여 200μM PTU(phenylthiourea)를 사용하였다. 그 후, 각 well에 1ug/ml의 α-멜라노사이트 자극 호르몬(α-melanocyte-stimulating hormone, α-MSH)를 처리하여 24시간동안 멜라닌 생성을 유도하고, 실시예 1에서 제조된 무궁화 꽃잎 추출물(PTS) 및 실시예 2에서 제조된 무궁화 꽃잎 추출물(PS)을 각각 0ug/ml, 25ug/ml, 100ug/ml, 400ug/ml의 농도로 처리한 후 48시간동안 배양하고, 각 well을 입체 현미경으로 촬영하여 멜라닌 합성 정도를 확인하였다. 또한, 도 3 및 도 4에 기재된 V는 vehicle control로 무궁화 꽃잎 추출물을 녹인 DMSO(0.001%)를 뜻한다.The mature zebrafish male and female were placed in an egg collection tank the day before the eggs were collected, and the eggs were collected after 1-2 hours after the Gwangju period the next day. The collected eggs were placed in a zebrafish embryo medium and generated for 48 hours, and then the embryos of zeppefish were collected and the embryos were distributed to each of 24-well plates using a pipette. 200 μM PTU (phenylthiourea) was used to form a transparent zeppefish in each well. Thereafter, 1 well/ml of α-melanocyte-stimulating hormone (α-MSH) was treated in each well to induce melanin production for 24 hours, and the green light petal extract prepared in Example 1 ( PTS) and the green light petal extract (PS) prepared in Example 2 were treated at concentrations of 0 ug/ml, 25 ug/ml, 100 ug/ml, and 400 ug/ml, respectively, and cultured for 48 hours, and each well was subjected to a stereoscopic microscope. It was photographed to confirm the degree of melanin synthesis. In addition, V described in FIGS. 3 and 4 means DMSO (0.001%) in which the green light petal extract is dissolved by vehicle control.
도 3은 실시예 1에서 제조된 무궁화 꽃잎 추출물(PTS) 처리에 따른 입체 현미경으로 제브라피쉬 배아를 촬영한 사진으로서, 멜라닌 생성을 유도하고, 실시예 1에서 제조된 무궁화 꽃잎 추출물을 400ug/ml의 농도로 처리할 때, 멜라닌 형성량이 저해됨을 확인할 수 있었다. 또한, α-MSH의 자극에 의해서 증가된 멜라닌합성이 실시예 1에서 제조된 무궁화 추출물(PTS)에 의해서 강력히 저해 됨을 확인 할 수 있었다.FIG. 3 is a photograph of a zebrafish embryo under a stereoscopic microscope according to the green light petal extract (PTS) treatment prepared in Example 1, inducing melanin production, and the green light petal extract prepared in Example 1 of 400 ug/ml When treated at a concentration, it was confirmed that the amount of melanin formation was inhibited. In addition, it was confirmed that increased melanin synthesis by stimulation of α-MSH was strongly inhibited by the green light extract (PTS) prepared in Example 1.
또한, 도 4은 실시예 2에서 제조된 무궁화 꽃잎 추출물(PS) 처리에 따른 입체 현미경으로 제브라피쉬 배아를 촬영한 사진으로서, 실시예 1에서 제조된 무궁화 꽃잎 추출물을 400ug/ml의 농도로 처리할 때, 멜라닌 형성량이 강력하게 저해됨을 확인할 수 있었다. 또한, α-MSH의 자극에 의해서 증가된 멜라닌합성이 실시예 1에서 제조된 무궁화 추출물(PS)에 의해서 강력히 저해 됨을 확인 할 수 있었다.In addition, FIG. 4 is a photograph of a zebrafish embryo under a stereoscopic microscope according to the treatment of the green light petal extract (PS) prepared in Example 2, wherein the green light petal extract prepared in Example 1 is processed at a concentration of 400 ug/ml. At this time, it was confirmed that the amount of melanin formation was strongly inhibited. In addition, it was confirmed that increased melanin synthesis by stimulation of α-MSH was strongly inhibited by the green light extract (PS) prepared in Example 1.
본 발명의 단순한 변형이나 변경은 이 분야의 통상의 지식을 가진 자에 의해서 용이하게 실시될 수 있으며, 이러한 변형이나 변경은 모두 본 발명의 영역에 포함되는 것으로 볼 수 있다.Simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes can be considered to be included in the scope of the present invention.
Claims (15)
상기 무궁화 꽃잎에 에탄올을 첨가하고, 암조건에서 정치 추출하여 무궁화 꽃잎의 알코올 추출물을 수득하는 제2단계; 및
상기 알코올 추출물을 감압 농축하여 무궁화 꽃잎 추출물을 제조하는 제3단계; 를 포함하고,
상기 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래인 것을 특징으로 하는 무궁화 꽃잎 추출물의 제조방법.
The first step of preparing the petals of green light;
A second step of adding ethanol to the green light petals, and subjecting the extract to dark conditions to obtain an alcoholic extract of the green light petals; And
A third step of concentrating the alcohol extract under reduced pressure to prepare a green light petal extract; Including,
The method of manufacturing the green light petal extract, wherein the green light petal extract is derived from the green light'sandalwood' (Scientific name: Hibiscus syriacus'Paektanshim ') or the green light'Firebird' (Scientific name: Hibiscus syriacus'Pulsae ').
상기 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제하는 것을 특징으로 하는 무궁화 꽃잎 추출물의 제조방법.
According to claim 1,
The green light petal extract manufacturing method of the green light petal extract characterized in that to inhibit the production of melanin (melanin).
상기 무궁화 꽃잎 추출물은 피부 미백용 또는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용인 것을 특징으로 하는 무궁화 꽃잎 추출물의 제조방법.
According to claim 1,
The green light petal extract is a method for preparing a light green flower petal extract for skin whitening or prevention or improvement of melanin hyperpigmentation disease.
상기 무궁화 꽃잎 추출물은 무궁화 꽃잎에 에탄올을 첨가하고, 암조건에서 정치 추출한 알코올 추출물이며,
상기 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래인 것을 특징으로 하는 피부 미백용 화장료 조성물.
Contains green light petal extract as an active ingredient,
The green light petal extract is an alcohol extract, in which ethanol is added to the green light petals, and the extract is statically extracted under dark conditions.
The Hibiscus petal extracts of Hibiscus' hundred single fibers (the scientific name: Hibiscus syriacus' Paektanshim ') or a rose of Sharon, Phoenix (scientific name: Hibiscus syriacus' Pulsae') a cosmetic composition for skin whitening, characterized in that the origin.
상기 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제하는 것을 특징으로 하는 피부 미백용 화장료 조성물.
The method of claim 5,
The green light petal extract is a cosmetic composition for skin whitening, characterized in that to inhibit the production of melanin (melanin).
상기 무궁화 꽃잎 추출물을 100 ~ 600ug/ml의 농도로 포함하는 것을 특징으로 하는 피부 미백용 화장료 조성물.
The method of claim 5,
Cosmetic composition for skin whitening, characterized in that it comprises the green light petal extract at a concentration of 100 ~ 600ug / ml.
상기 무궁화 꽃잎 추출물은 무궁화 꽃잎에 에탄올을 첨가하고, 암조건에서 정치 추출한 알코올 추출물이며,
상기 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래인 것을 특징으로 하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물.
Contains green light petal extract as an active ingredient,
The green light petal extract is an alcohol extract, in which ethanol is added to the green light petals, and the extract is statically extracted under dark conditions.
The green light petal extract is derived from a light green'sandalwood' (Scientific name: Hibiscus syriacus'Paektanshim ') or a green light'Firebird' (Scientific name: Hibiscus syriacus'Pulsae '). Composition.
상기 멜라닌 색소 과다 침착 질환은 주근깨, 노인성 반점, 간반, 기미, 흑점, 일광색소반, 푸른흑피증(cyanic melasma), 약물 사용 후의 과다색소침착, 임신성 갈색반(gravidic chloasma), 또는 상처 또는 피부염으로 인한 염증 후 과다 색소 침착인 것을 특징으로 하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물.
The method of claim 9,
The melanin hyperpigmentation disease may include freckles, senile plaques, liver spots, blemishes, black spots, sun pigmentation, cyano melasma, hyperpigmentation after drug use, gestational chloasma, or wounds or dermatitis. A pharmaceutical composition for preventing or improving melanin hyperpigmentation disease, which is characterized by hyperpigmentation after inflammation.
상기 무궁화 꽃잎 추출물은 멜라닌(melanin)의 생성을 억제하는 것을 특징으로 하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물.
The method of claim 9,
The green light petal extract is a pharmaceutical composition for preventing or improving melanin hyperpigmentation disease, characterized by inhibiting the production of melanin.
상기 무궁화 꽃잎 추출물을 100 ~ 600ug/ml의 농도로 포함하는 것을 특징으로 하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 약학조성물.
The method of claim 9,
Pharmaceutical composition for the prevention or improvement of melanin hyperpigmentation disease, characterized in that it contains the green light petal extract at a concentration of 100 ~ 600ug / ml.
상기 무궁화 꽃잎 추출물은 무궁화 꽃잎에 에탄올을 첨가하고, 암조건에서 정치 추출한 알코올 추출물이며,
상기 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래인 것을 특징으로 하는 피부 미백용 건강기능성 식품.
Contains green light petal extract as an active ingredient,
The green light petal extract is an alcohol extract, in which ethanol is added to the green light petals, and the extract is statically extracted under dark conditions.
The Hibiscus petal extracts of Hibiscus' back single-core (scientific name: Hibiscus syriacus' Paektanshim ') or Sharon' The Firebird '(Scientific name: Hibiscus syriacus' Pulsae') functional health food for skin whitening comprising an origin.
상기 무궁화 꽃잎 추출물은 무궁화 꽃잎에 에탄올을 첨가하고, 암조건에서 정치 추출한 알코올 추출물이며,
상기 무궁화 꽃잎 추출물은 무궁화 '백단심'(학명 : Hibiscus syriacus 'Paektanshim') 또는 무궁화 '불새'(학명 : Hibiscus syriacus 'Pulsae') 유래인 것을 특징으로 하는 멜라닌 색소 과다 침착 질환의 예방 또는 개선용 건강기능성 식품.
Contains green light petal extract as an active ingredient,
The green light petal extract is an alcohol extract, in which ethanol is added to the green light petals, and the extract is statically extracted under dark conditions.
The green light petal extract is derived from green light'sandalwood' (scientific name: Hibiscus syriacus'Paektanshim ') or green light'firebird' (scientific name: Hibiscus syriacus'Pulsae '). Functional food.
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| KR20240073362A (en) | 2022-11-18 | 2024-05-27 | 국립낙동강생물자원관 | Composition for skin whitening comprising cherry tree extract as an active ingredient |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102299846B1 (en) * | 2021-03-04 | 2021-09-09 | 농업회사법인 (주)로가 | Cosmetic composition comprising collagen peptides derived from Hibiscus |
| KR102366932B1 (en) * | 2021-03-17 | 2022-02-25 | (주)에스디생명공학 | Composition with Antioxidant, Anti-Inflammation, Skin Moisturizing, Anti-Wrinkle, and Skin Regeneration Property Comprising Complex Extract of Hibiscus Syriacus Softened Petal as Active Ingredient |
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| JP2003171300A (en) * | 2001-09-28 | 2003-06-17 | Kobayashi Pharmaceut Co Ltd | Melanization inhibitor |
| JP2006306863A (en) | 2005-03-31 | 2006-11-09 | Kobayashi Pharmaceut Co Ltd | Melanin formation inhibitor |
| KR100993603B1 (en) * | 2008-04-11 | 2010-11-10 | 한국농수산대학 산학협력단 | Extracts of Paeoniae radix flower with whitening and Manufacturing Method |
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| KR101849725B1 (en) | 2016-06-09 | 2018-04-18 | 주식회사 루비크라운 | Peptides with melanogenesis-inhibiting activity and compositions thereof |
| KR101902317B1 (en) | 2016-09-06 | 2018-10-01 | 태이생명과학 주식회사 | Enzyme treated Mugunghwa extracts for improving skin wrinkle |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2003171300A (en) * | 2001-09-28 | 2003-06-17 | Kobayashi Pharmaceut Co Ltd | Melanization inhibitor |
| JP2006306863A (en) | 2005-03-31 | 2006-11-09 | Kobayashi Pharmaceut Co Ltd | Melanin formation inhibitor |
| KR100993603B1 (en) * | 2008-04-11 | 2010-11-10 | 한국농수산대학 산학협력단 | Extracts of Paeoniae radix flower with whitening and Manufacturing Method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20240073362A (en) | 2022-11-18 | 2024-05-27 | 국립낙동강생물자원관 | Composition for skin whitening comprising cherry tree extract as an active ingredient |
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