KR102078823B1 - Cosmetic composition for skin moisturizing with blue pearl protein - Google Patents

Cosmetic composition for skin moisturizing with blue pearl protein Download PDF

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KR102078823B1
KR102078823B1 KR1020190075879A KR20190075879A KR102078823B1 KR 102078823 B1 KR102078823 B1 KR 102078823B1 KR 1020190075879 A KR1020190075879 A KR 1020190075879A KR 20190075879 A KR20190075879 A KR 20190075879A KR 102078823 B1 KR102078823 B1 KR 102078823B1
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protein
ultrafiltration
pearl
skin
blue
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KR1020190075879A
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Korean (ko)
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홍화영
최봉규
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홍화영
최봉규
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/96Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/30Cosmetics or similar toilet preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/007Preparations for dry skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The present invention relates to a cosmetic composition having a skin moisturizing effect using blue pearl protein as an active ingredient, and the present invention provides a cosmetic composition containing blue pearl protein as an active ingredient obtained by filtration after fermentation with lactic acid bacteria to provide excellent skin moisturizing effect. It can be effective.

Description

Cosmetic composition for skin moisturizing with blue pearl protein

The present invention relates to a cosmetic composition having a skin moisturizing effect containing blue pearl (blue pearl) protein as an active ingredient.

Since ancient times, Pearl is a precious and old jewel that is said to be the origin of herbal medicine by paying attention to its medicinal effects, soothing the mind, and moisturizing when applied to the face and disappearing the boil of the skin. The main ingredient of pearl consists of 90 ~ 95% calcium carbonate, 5% protein (conchiolin), 20 kinds of minerals and amino acids which are various physiologically active substances, and these ingredients are widely used as health supplements or skin beauty ingredients. have. Pearls come in a variety of varieties, including freshwater pearls, conk pearls, agoya pearls, and south sea pearls.

Conchiolin, a protein component in pearls, is known to have effects such as wound healing. As a method for obtaining such conchiolin, the deliming process through acid hydrolysis of acetic acid and the like has been mainly performed in the existing studies. However, a process using an acid such as acetic acid has problems such as irritation upon skin contact. Therefore, there is a need to develop a new process that is excellent in skin stability and effectively exhibits the skin-related effects of conchiolin.

Republic of Korea Patent Publication No. 10-2018-0087592 (published date: 2018.08.02) relates to a cosmetic composition for skin whitening, wrinkle improvement or skin regeneration comprising a gujeukpo ginseng extract and a water-soluble pearl as an active ingredient, skin whitening, Wrinkle improvement or skin regeneration effect is disclosed.

An object of the present invention is to develop and provide a cosmetic composition having a skin moisturizing effect containing blue pearl protein obtained by filtration after fermentation with lactic acid bacteria as an active ingredient.

The present invention is characterized in that it contains a protein obtained by filtering the supernatant of the fermentation product obtained by fermenting blue pearl with Acetobacter genus or Lactobicillus genus Lactobacillus ( Lactobicillus ) or the supernatant as an active ingredient. Provided is a cosmetic composition for moisturizing the skin.

On the other hand, in the present invention, the acetobacter genus acetic acid is preferably acetobacter aceti ( Acetobacter aceti ).

On the other hand, in the present invention, the Lactobacillus genus lactic acid bacteria, preferably one or more selected from Lactobicillus fermentum ( Lactobicillus fermentum ), Lactobicillus delbrueckii ( Lactobicillus delbrueckii ), Lactobicillus plantarum ( Lactobicillus plantarum ) It is good.

The present invention can provide an excellent skin moisturizing effect by providing a cosmetic composition containing the blue pearl protein obtained by filtration after fermentation with lactic acid bacteria as an active ingredient.

1 is a graph showing the results of evaluation of the cytotoxicity of the blue pearl protein and gold pearl protein obtained by ultrafiltration after microbial fermentation of the present invention.
Figure 2 is a graph of the experimental results confirming the molecular weight of the blue pearl protein and gold pearl protein obtained by ultrafiltration after microbial fermentation of the present invention.
3 is a graph showing experimental results confirming the molecular weight of the comparative example.
Figure 4 is a graph showing the results of the measurement of hyaluronic acid production of blue pearl protein and gold pearl protein obtained by ultrafiltration after microbial fermentation of the present invention.

Pearl is a bead-like or hemispherical glossy abnormal secretion mainly composed of calcium carbonate produced in the shell of shellfish, and it is one of the gems that has been favored since ancient times as a symbol of health, wealth and longevity. Pearls are evaluated by the clarity of surface gloss, degree of sphericality, purity, color, and the like. The main ingredient of pearl consists of 90 ~ 95% calcium carbonate, 5% protein (conchiolin), 20 kinds of minerals and amino acids which are various physiologically active substances, and these ingredients are widely used as health supplements or skin beauty ingredients. have.

Conchiolin, a protein component in pearls, is known to have effects such as wound healing. As a method for obtaining such conchiolin, the deliming process through acid hydrolysis of acetic acid and the like has been mainly performed in the existing studies. However, the existing process using acetic acid, etc. has a limitation that there is a problem such as irritation upon skin contact. Therefore, the present invention has developed a process that is excellent in skin stability, and can effectively exhibit the skin-related effects of conchiolin.

Accordingly, the present invention is characterized by containing the protein obtained by filtering the supernatant or the supernatant of the fermentation obtained by fermenting blue pearl with Acetobacter genus or Lactobicillus lactic acid bacteria as an active ingredient. It provides a cosmetic composition for moisturizing the skin.

The blue pearl of the present invention is a pearl obtained from a shellfish called Haliotis iris of New Zealand, called Paua in the native Maori (Paori) language, and Paua (Paua) among three abalone species. ) Is a species that is commercially caught and farmed. Paua feeds on nutritious algae, which is always rich in energy because of high quality New Zealand algae, which contain a variety of nutrients from high and low tide.

Meanwhile, in the present invention, the acetobacter genus acetic acid is preferably Acetobacter aceti, and more preferably Acetobacter aceti ATCC 15973.

Chosangyun by oxidizing bacteria (Acetobacter) and glucose making acetic acid by oxidation of the alcohol is divided into gluconate or bacteria Kane (Gluconobacter) to create acid. Acetobacter , which is mainly involved in the production of candles, is an aerobic bacterium, which varies depending on the type of ellipse or single phase, and is thus referred to as 'acetic acid fermentation'. Since the conventional hydrolysis of pulp protein (pearl protein) was performed using acetic acid, acetic acid bacteria were first selected as a fermentation strain and used in the present invention.

On the other hand, the Lactobacillus genus lactic acid bacteria, preferably Lactobacillus fermentum ( Lactobicillus fermentum ), Lactobicillus delbrueckii ( Lactobicillus delbrueckii ), Lactobicillus plantarum ( Lactobicillus plantarum ) is preferably any one or more selected from, more preferably. Preferably, at least one selected from Lactobicillus fermentum ATCC 9338 , Lactobicillus delbrueckii ATCC 11842, and Lactobicillus plantarum ATCC 8014.

Lactic acid bacteria, also known as lactic acid bacteria, is a generic term for bacteria that ferment sugars to obtain energy and produce large amounts of lactic acid. It is used as a probiotic that plays a role in preventing the growth of harmful bacteria and maintaining the balance of microbial communities. Representative lactic acid bacteria used as probiotics, Lactobicillus ( Lactobicillus ), Bifidobacterium ( Bifidobacterium ), Lactococcus ( Lactococcus ), Leuconostoc (Leuconostoc), Pediococcus ( Pediococcus ) and the like are used a lot. Lactobacillus is known as a glass (GLAS) strain that is harmless to the human body, in the present invention, the lactic acid bacteria were selected and used as a fermentation strain.

In the present invention, 'filtration' may use a conventional method, and preferably, ultrafiltration may be used.

According to the following experiment, it was confirmed that the blue pulp protein obtained by filtration after the fermentation of the present invention exhibits a skin moisturizing effect by increasing the production of hyaluronic acid, which maintains the moisture content of the skin with strong hydrophilicity. By the way, the hydrolysis method using acetic acid is widely known in the prior art, the method of fermentation with acetic acid bacteria was predicted in advance that the result will be better than the fermentation with lactic acid bacteria, the actual results of the present invention, using the lactic acid bacteria rather than fermentation products using acetic acid bacteria Fermented products exhibited a higher skin moisturizing effect.

In addition, the gold pearl protein obtained by filtration after lactic acid bacteria fermentation in addition to the blue pulp protein of the present invention was also confirmed to exert a skin moisturizing effect by increasing the production of hyaluronic acid, the use of blue pulp protein than using the gold pulp protein It has been shown to exert a higher skin moisturizing effect.

On the other hand, the cosmetic composition of the present invention, for example, solutions, suspensions, emulsions, pastes, lotions, gels, water-soluble liquids, creams, essences, surfactant-containing cleansing, oil, oil-in-water (O / W) type and oil One of the basic cosmetic formulations selected from heavy water (W / O) type; skin; Lotion; Eye cream; Soothing gel; Ointment; Formulation for mask pack; Body wash formulations; Peeling gel; Oil-in-water and water-in-oil makeup bases; foundation; Skin cover; Color cosmetic formulations selected from lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencils; Scalp formulations; It may be any one selected from among.

Moreover, the cosmetic composition of this invention can be used overlapping with other cosmetic compositions other than this invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may vary depending on the skin condition or taste of the user.

Hereinafter, the content of the present invention will be described in more detail through the following examples or experimental examples. However, the scope of the present invention is not limited only to the following examples and experimental examples, but includes modifications of equivalent technical ideas.

Example 1 Preparation of Blue Pearl Protein Obtained by Ultrafiltration after Microbial Fermentation

1) Acetobacter ( Acetobacter ) Blue Pearl Protein obtained by Ultrafiltration after Fermentation (Example 1-1)

Acetobacter aceti ATCC 15973 strains were inoculated in Mannitol Broth medium, and then incubated in a 25 ° C. shaking incubator for 48 hours.

400 ml of Mannitol Broth medium was inoculated with 1 x 10 4 cfu / ml of Acetobacter aceti ATCC 15973 cultured above, sterilized at 121 ° C. for 15 minutes, and then 48 ° C. at 40 ° C. It was mixed with 30 g of dry blue pearl powder. Then, incubated for 48 hours in a 25 ℃ shaking incubator (shaking incubator).

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

2) Lactobacillus ( Lactobacillus ) Blue Pearl Protein obtained by Ultrafiltration after Fermentation (Example 1-2)

Lactobacillus bacteria (Lactobacillus) MRS Broth medium Lactobacillus momentum spread (Lactobacillus fermentum ATCC 9338) then inoculated with the strain, 25 ℃ shaking incubator (shaking incubator) for a cultured 48 hours.

Lactobacillus fermentum ATCC 9338 cultured in MRS Broth medium 400ml inoculated to 1 x 10 4 cfu / ml strain, sterilized for 15 minutes at 121 ℃, 15 minutes 30g blue pearl powder dried for 48 hours at 40 ℃ Mixed with. 48 hours incubation in a 25 ℃ shaking incubator (shaking incubator).

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Example 2 Preparation of Gold Pearl Protein Obtained by Ultrafiltration after Microbial Fermentation

1) Acetobacter ( Acetobacter ) Gold Pearl Protein Obtained by Ultrafiltration after Fermentation (Example 2-1)

Acetobacter aceti ATCC 15973 strains were inoculated in Mannitol Broth medium, and then incubated in a 25 ° C. shaking incubator for 48 hours.

400 ml of Mannitol Broth medium was inoculated with 1 x 10 4 cfu / ml of Acetobacter aceti ATCC 15973 cultured above, sterilized at 121 ° C. for 15 minutes, and then 48 ° C. at 40 ° C. It was mixed with 30 g of dry gold pearl powder. Then, the culture was incubated for 48 hours in a 25 ℃ shaking incubator (shaking incubator).

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

2) Lactobacillus ( Lactobacillus ) Gold pearl protein obtained by ultrafiltration after fermentation (Example 2-2)

Lactobacillus bacteria (Lactobacillus) MRS Broth medium Lactobacillus momentum spread (Lactobacillus fermentum ATCC 9338) then inoculated with the strain, 25 ℃ shaking incubator (shaking incubator) for a cultured 48 hours.

Lactobacillus fermentum ATCC 9338 cultured in 400 ml MRS Broth medium inoculated to 1 x 10 4 cfu / ml strain, sterilized for 15 minutes at 121 ℃, 15 minutes 30g dried gold pearl powder for 48 hours Mixed with. 48 hours incubation in a 25 ℃ shaking incubator (shaking incubator).

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Comparative Example 1: Preparation of Water-Soluble White Pearl Powder

Water-soluble white pearl powder was used by purchasing a commercial product.

Comparative Example 2: Preparation of White Pearl Powder Acetic Acid Hydrolysate

After sterilization at 121 ° C. for 15 minutes, 400 ml of 40% (w / w) acetic acid was added to 30 g of white pearl powder dried at 40 ° C. for 48 hours and reacted at 37 ° C. for 16 hours.

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Comparative Example 3: Preparation of White Pearl Powder Lactic Acid Hydrolysate

After sterilization at 121 ° C. for 15 minutes, 400 ml of 40% (w / w) lactic acid was added to 30 g of white pearl powder dried at 40 ° C. for 48 hours and reacted at 37 ° C. for 16 hours.

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Comparative Example 4: Preparation of Gold Pearl Protein Acetic Acid Hydrolysate

After sterilization at 121 ° C. for 15 minutes, 400 ml of 40% (w / w) acetic acid was added to 30 g of gold pearl powder dried at 40 ° C. for 48 hours and reacted at 37 ° C. for 16 hours.

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Comparative Example 5: Preparation of Gold Pearl Protein Lactic Acid Hydrolysate

After sterilization at 121 ° C. for 15 minutes, 400 ml of 40% (w / w) lactic acid was added to 30 g of gold pearl powder dried at 40 ° C. for 48 hours and reacted at 37 ° C. for 16 hours. After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Comparative Example 6: Preparation of Blue Pearl Protein Acetic Acid Hydrolyzate

After sterilization at 121 ° C. for 15 minutes, 400 ml of 40% (w / w) acetic acid was added to 30 g of blue pearl powder dried at 40 ° C. for 48 hours and reacted at 37 ° C. for 16 hours.

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Comparative Example 7: Preparation of Blue Pearl Protein Lactic Acid Hydrolyzate

After sterilization at 121 ° C. for 15 minutes, 400 ml of 40% (w / w) lactic acid was added to 30 g of blue pearl powder dried at 40 ° C. for 48 hours and reacted at 37 ° C. for 16 hours.

After calibrating to pH5.8-6.0 using 1 M NaOH, the mixture was centrifuged at 4 ° C. and 4000 rpm. The supernatant was taken and an ultrafiltration device (Ultrafiltration) was used to obtain 500 ml of protein (protein) -containing filtrate having a molecular weight of 30 kDa or less.

Experimental Example 1: Evaluation of Cytotoxicity of Blue Pearl Protein and Gold Pearl Protein Obtained by Ultrafiltration after Microbial Fermentation (MTT assay)

In this experimental example, the cytotoxicity of Blue Pearl Protein and Gold Pearl Protein of Example 1 and Example 2 was evaluated.

Human-derived fibroblasts (Human derma fibroblast, HDF) were inoculated in a 96-well plate at 0.5x10 4 cells / well and incubated for 24 hours. The wells were incubated for 24 hours. After 24 hours, 20 μl of 5 mg / ml MTT reagent was added per well, followed by 2 hours of incubation. After removing all the medium containing the MTT reagent and the sample, 100 μl of isopropanol was added to each well, followed by a multi plate reader. ) The cell survival rate was calculated by measuring the absorbance value (OD) at 570nm. Cytotoxicity is considered to be less than 80% cell viability.

As a result, as shown in FIG. 1, both the Comparative Example and the Example group had cell viability of 80% or more at the experimental concentration, and it was confirmed that no cytotoxicity was observed.

Experimental Example 2: Confirmation of Molecular Weight of Blue Pearl Protein and Gold Pearl Protein Obtained by Ultrafiltration after Microbial Fermentation

In the present experimental example was to check the molecular weight of the blue pearl protein and gold pearl protein of Example 1 and Example 2.

Example 1 (1-1, 1-2), Example 2 (2-1, 2-2) and Comparative Examples 1 to 7 were subjected to gel permeation chromatography (0.45 μm Nylon filtration) under the following conditions. After molecular permeation chromatography (GPC) analysis, the molecular weight of each sample was calculated. This experiment was conducted by Koptri, Korea Polymer Testing Institute. Detailed conditions are as follows.

(1) Analyzer: Tosoh EcoSEC HLC-8320 GPC

(2) Detector: RI-detector

(3) Solvent: 0.1 M NaNO 3

(4) Column: 2 X TSKgel GMPWxl + TSKgel G2500PWxl (7.8 x 300 mm)

(5) Temperature: 40 ℃

(6) flow rate: 1.0 ml / min

(7) Injection amount: 100 μl

(8) Standard: PEG / PEO

(9) Data calculation: EcoSEC software

As a result of molecular weight measurement using GPC, when checking the average molecular weight as shown in Tables 1 and 2, Figures 2 and 3, Example 1 (1-1, 1-2) and Example 2 (2-1, 2- 2), significant molecular weight differences of Comparative Examples 1 to 7 were not confirmed, but it was confirmed that they were distributed at very small molecular weights.

Sample group Example
1-1
Example
1-2
Example
2-1
Example
2-2
Molecular Weight
(Da)
- 1250 586 850

Sample group Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Molecular Weight
(Da)
855 882 802 558 798 649 842

Experimental Example 3: Confirmation of Skin Moisturizing Effect of Blue Pearl Protein and Gold Pearl Protein Obtained by Ultrafiltration after Microbial Fermentation

Hyaluronic Acid (Hyaluronan, HA) production of the major moisturizing factors present in the skin to confirm the skin moisturizing efficacy of the blue pearl protein and gold pearl protein of Examples 1 and 2 to smooth the skin moisture barrier The effect was confirmed.

As a major moisturizing factor present in the skin, the effect of the sample on the production of hyaluronic acid (Hyaluronan, HA), which smoothes the skin's moisture retention barrier, was confirmed. Human-derived fibroblasts (Human derma fibroblast, HDF) were inoculated in 6-well plates at 1x10 5 cells / well and incubated for 24 hours, Comparative Examples and Examples were added to serum-free medium containing 50 and 100 ㎍ / ml, respectively Treated for 48 hours. After 48 hours, the amount of hyaluronic acid secreted in the culture was reacted according to the protocol using an immunoassay kit (R & D system), and then the absorbance value (OD) was measured at 450 nm using a multi plate reader. Measured.

As a result of the hyaluronic acid production measurement, as shown in Figure 4, when treated with 50 µg / ml and 100 µg / ml, Example 1-1 and Example 1-2 are the blue pulp protein material compared to other groups The production rate was high, and the concentration of hyaluronic acid was increased. In addition, Example 2-2 of the gold pearl protein obtained by ultrafiltration after lactic acid bacteria fermentation was confirmed to increase the production of hyaluronic acid. Through this, it was confirmed that using blue pearl protein showed a higher skin moisturizing effect than using gold pearl protein.

On the other hand, the hyaluronic acid production rate of Blue Pearl Protein Examples 1-1 (acetic acid fermentation product) and Example 1-2 (Lactic acid bacteria fermentation product) of the present invention is higher than that of Comparative Example 6 and Comparative Example 7, which are acid hydrolysates of blue pearl protein. It was confirmed that the high, the hyaluronic acid production rate of the protein obtained by ultrafiltration after lactic acid bacteria fermentation ultrafiltration than the ultrafiltration after acetic acid fermentation was confirmed to exhibit a higher skin moisturizing effect.

Claims (3)

  1. The blue pearls are fermented with one or more lactic acid bacteria selected from Lactobicillus fermentum , Lactobicillus delbrueckii , Lactobicillus plantarum and neutralized, followed by centrifugation and supernatant. After obtaining, the method of producing a skin moisturizing cosmetic composition comprising filtering the supernatant to obtain a protein having a molecular weight of 30 kDa or less.
  2. delete
  3. delete
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050040491A (en) * 2003-10-29 2005-05-03 주식회사 태평양 Composition for external application to the skin containing the nano capsule of pearl protein
KR101614659B1 (en) * 2015-10-12 2016-04-21 이충식 Manufacturing method for functional cosmetics
KR20180033061A (en) * 2017-09-06 2018-04-02 샘표식품 주식회사 Methods for Improving Flavors of Fermented Vinegar and Composition for Improving Skin Conditions Comprising Pearl Fermentation Products Thereby
KR20180070886A (en) * 2016-12-19 2018-06-27 주식회사 코리아나화장품 Cosmetic composition comprising organic acid fermentation and natural polymer for moisturizing effect on the skin
KR20180087592A (en) 2017-01-25 2018-08-02 김보경 Cometic composition for skin whitening, anti-wrinkle and skin regeneration comprising nine times-steaming ginseng extract and water-soluble pearl as active ingredient

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050040491A (en) * 2003-10-29 2005-05-03 주식회사 태평양 Composition for external application to the skin containing the nano capsule of pearl protein
KR101614659B1 (en) * 2015-10-12 2016-04-21 이충식 Manufacturing method for functional cosmetics
KR20180070886A (en) * 2016-12-19 2018-06-27 주식회사 코리아나화장품 Cosmetic composition comprising organic acid fermentation and natural polymer for moisturizing effect on the skin
KR20180087592A (en) 2017-01-25 2018-08-02 김보경 Cometic composition for skin whitening, anti-wrinkle and skin regeneration comprising nine times-steaming ginseng extract and water-soluble pearl as active ingredient
KR20180033061A (en) * 2017-09-06 2018-04-02 샘표식품 주식회사 Methods for Improving Flavors of Fermented Vinegar and Composition for Improving Skin Conditions Comprising Pearl Fermentation Products Thereby

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