KR102044221B1 - Multi-system for conducting biochemistry and immunoassey analysis - Google Patents

Abstract

The present invention provides a multi-system for simultaneously performing a biochemical test and an immune response test, comprising: a mobile unit moving in front, rear, left, and right directions; And coupled to the lower end of the mobile unit, the tube coupling portion 30 for detachably coupling at least two tubes 50; characterized in that comprises a, using a multi-system according to the invention, blood cells Except for morphological tests to count the number of biochemical tests, immune serum tests, special chemical tests, and genetic tests, it is possible to simultaneously test all the devices on a single device.

Description

Multi-system for conducting biochemistry and immunoassey analysis

The present invention relates to a multi-system for simultaneously performing a biochemical test and an immune response test, and more particularly, biochemical test, immunoserum test, special chemical test and genetic test except morphological test method for counting the number of blood cells. The present invention relates to a multi-system for simultaneously performing biochemical tests and immune response tests, which are configured to simultaneously test all of a single device.

Because blood plays an important role in supplying oxygen, nutrients, hormones and nutrients to cells of all organs in the body, it also acts as a defense against infection and transports toxins and wastes. It will change the ingredients. Therefore, various components of blood should be examined to accurately diagnose, treat, and prognose the disease.

In order to test the blood as described above, a special chemical test is performed along with morphological, biochemical and immunoserum tests of the collected blood.

Here, the morphological test of blood is a blood cell test and a blood smear test that measures the hemoglobin, hematocrit (blood cell volume), erythrocytes, white blood cells, platelet count, and the percentage of cells in the white blood cell using whole blood (smear the blood thinly on a slide and staining it). Direct observation of the shape and number of blood cells under a microscope), bleeding time, coagulation time, capillary resistance, etc., to check for bleeding tendency, blood disease infection, and the course and prognosis of various diseases. In this case, the measurement is performed using an electrochemical method and a dyeing method.

In addition, biochemical methods, immunological methods, and special chemical methods are first used to remove blood cells in the blood by centrifugation or the like, and the test is performed as a supernatant (serum or plasma).

In general, the biochemical method is mainly to react the serum (or plasma) to each indicator, and then to measure the absorbance of a specific wavelength to convert to a concentration, wet and dry depending on the type of indicator used. Divided.

Wet is the method of choice for most people, where the indicator is in liquid form, causing it to react with plasma. In the dry method, the indicator is solidified and coated on a thin film for easier measurement, and then the absorbance is measured by reacting it with serum (plasma).

In addition, the immunoserum test is a test for antigens and antibodies to various bacteria and viruses to diagnose B, hepatitis C, AIDS, syphilis, and the like, and also to diagnose rheumatoid disease and to function various immune cells in the blood. This is done by separating and measuring the specific immunoglobulin in the blood for various causative agents of allergies to reveal the cause of allergies.

In most immunoserum tests, a method of measuring the amount of the antibody (antigen) by labeling a marker to the antibody (antigen) generally to be tested by using an antigen-antibody immune response is adopted.

Specialty chemical tests are used to diagnose myocardial infarction, hyperthyroidism and hypothyroidism, and various metabolites for congenital or acquired metabolic disorders. These tests include cancer marker tests, which do not directly diagnose specific cancers. It can be used as a supplementary tool for the diagnosis of cancer, and most of the current tests are performed in a form similar to the immunoserum test.

Recently, a lot of technologies have been developed to perform a large amount of specimens at the same time, such as DNA chip or lab-on-a-chip. These methods are mainly a method for simultaneously performing an immunoserum test and a genetic test.

In the same way, the development of test equipment and kits that can carry out biochemical tests, immunoserum tests, special chemical tests, and genetic tests at the same time, except for morphological test methods for counting blood cells. This situation is desperately needed.

Korea Patent Registration No. 10-0748181 Korea Patent Registration No. 10-1608598

The present invention has been made to solve the above-described problems, an object of the present invention is to provide a multi-system configured to be able to test both biochemical tests and immune response tests in one device at the same time.

In order to solve the above problems, a multi-system that simultaneously performs a biochemical test and an immune response test according to the present invention,

A mobile unit moving in the front, rear, left, and right directions; And

It is coupled to the bottom of the mobile unit, the tube coupling portion 30 for detachably coupling at least two tubes 50; characterized in that it comprises a.

In addition, in the multi-system according to the invention,

The mobile unit,

A first movable body 10 moving forward or backward from side to side along the first guide rail fixedly mounted on the upper part of the apparatus, the second guide rail being elongated in the front-rear direction on one surface;

A second moving body 20 moving forward or backward along the second guide rail of the first moving body, the upper and lower guide protrusions being provided on one side thereof; And

And an upper and lower guide rails engaged with the upper and lower guide protrusions of the second movable body 20 to move up and down in the vertical direction.

In addition, in the multi-system according to the invention,

The multi-system,

And a control means for controlling the first moving body 10, the second moving body 20, the shanghai east part 35, and the tube coupling part 30.

In addition, in the multi-system according to the invention,

The East Shanghai 35,

It is characterized in that the tube coupling portion 30 is fixed at the bottom.

In addition, in the multi-system according to the invention,

The tube coupling portion 30,

An engaging body 31 having an upper portion open and a plurality of through holes formed in the bottom surface thereof;

Coupling assistance portion 32 provided to be spaced apart from both ends of the coupling portion body 31; And

It is characterized in that it comprises a; the first push rod (33) provided in the spaced apart space of the both side ends of the coupling portion body 31 and the coupling auxiliary portion (32) to move up and down.

In addition, in the multi-system according to the invention,

The coupling part body 31 is provided with at least two coupling grooves 31a on the bottom of the lower surface, characterized in that the coupling protrusion 51a of the first tube 51 is fitted.

In addition, in the multi-system according to the invention,

The coupling assistant 32 is provided with a binding member 32a on the bottom of the lower surface,

Removably coupled to the binding portion (52a) of the second tube (52).

In addition, in the multi-system according to the invention,

The binding member 32a and the binding portion 52a may be made of a magnetic material.

In addition, in the multi-system according to the invention,

The first push rod 33,

The binding member 32a and the second tube of the coupling assistant 32 are applied downward by applying a downward force to the second tube 52 which is fastened to the lower end of the coupling assistant 32 while being moved downward by the control means. It is characterized in that the binding portion 52a of the 52 is separated.

In addition, in the multi-system according to the invention,

A second pusher 40 having a plurality of tube guides 41 at the bottom thereof is detachably coupled to the coupling part body 31 of the tube coupling part 30.

In addition, in the multi-system according to the invention,

The second rod 40 is further provided with a shanghai Dongbu 45,

The shanghai copper auxiliary unit 45 is characterized in that it is coupled to move up and down in the vertical direction.

In addition, in the multi-system according to the invention,

The tube guide 41 of the second pusher 40,

It is coupled to the plurality of through-holes (30b) of the coupling portion body 31 so as to be movable in the vertical direction, to guide each strip well of the first tube (51) to be put in place, as well as to move downward The second pusher 40 is moved downward by separating the first tube 51 from the coupling part body 31.

In addition, in the multi-system according to the invention,

The tube 50 is composed of a second tube 52 sandwiched from the bottom of the first tube 51, the first tube 51 and the second tube 52 are each composed of a plurality of strip wells It is characterized by.

In addition, in the multi-system according to the invention,

A coupling protrusion 51a is formed on the upper flange of the first tube 51,

A detachable part 52a is formed on an extended surface of the upper flange of the second tube 52, and a bottom surface of each strip well of the second tube 52 is formed in a mesh structure.

Biochemical test, immunoserum test, except morphological test method for counting the number of blood cells, It is possible to test both special chemical tests and genetic tests on one device at the same time.

1 is a perspective view briefly showing the overall configuration of a multi-system for performing a biochemical test and immune response test according to an embodiment of the present invention.
Figure 2 is an enlarged perspective view showing a coupling state of the tube coupling portion of FIG.
3 is an exploded perspective view of FIG. 2;
4 is a schematic cross-sectional view of FIG.
5 is an exploded cross-sectional view of FIG.
6 (a), (b) is an operation diagram conceptually showing the operation of the second pusher in the tube coupling portion of FIG.
Figure 7 (a), (b), (c) is a view showing the coupling state and the tube stripping method of the tube coupling portion of the multi-system performing a biochemical test and immune response test according to an embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings will be described in detail a preferred embodiment of a multi-system for performing the present invention biochemical test and immune response test.

1 is an overall perspective view briefly showing the overall configuration of a multi-system for performing a biochemical test and immune response test according to an embodiment of the present invention, Figure 2 is an enlarged perspective view showing a coupling state of the tube coupling of Figure 1, Figure 3 2 is an exploded perspective view of FIG. 2, FIG. 4 is a schematic cross-sectional view of FIG. 2, FIG. 5 is an exploded cross-sectional view of FIG. 4, and FIGS. 6A and 6B are conceptual views illustrating the operation of the second pusher in the tube coupling part of FIG. 2. 7, (a), (b), (c) is shown in the coupling state and the tube removal method of the tube coupling portion of the multi-system performing a biochemical test and immune response test according to an embodiment of the present invention It is a figure which shows.

As shown in Figures 1 to 7, the multi-system (1) for simultaneously performing a biochemical test and immune response test according to the present invention includes a mobile unit and a tube coupling portion 30 coupled to the lower end of the mobile unit; It is configured by.

Here, the moving unit is a device for moving in the front-rear direction (X-axis direction), left-right direction (Y-axis direction) and up-down direction (Z-axis direction) by the control means (not shown), and the first moving body 10, The second moving body 20 and the shanghai east part 35 are comprised.

The first moving body 10 moves forward or backward along the first guide rail 11 fixed to the upper portion of the device in the left and right directions (Y-axis direction), and the first guide rail is disposed on the lower bottom surface of the first moving body 10. The first guide groove 13 is formed to be movable along the (11), and the second guide rail 12 (12; 12a, 12b) is formed on the upper surface in the front and rear (X-axis direction) long.

In the present invention, it is shown that a rectangular groove is formed in the first moving body 10 and the second guide rails 12 (12a, 12b) are formed on the upper surfaces of both moving bodies of the groove, but the present invention is not limited thereto. It is a matter of course that the shape of the movable body 10 may be formed of a bar-shaped facet or a frame, and guide rails may be formed on the side or bottom of the facet or frame.

The second movable body 20 (21, 22) moves forward or backward along the second guide rails 12 (12a, 12b) of the first movable body 10 in the forward and backward directions (X-axis direction), and the second movable body 20 A second guide groove 23 is formed on each of the lower bottom surfaces of the 21 and 22 to move along the second guide rails 12 and 12a and 12b, and an upper and lower guide protrusion 24 is formed on one side thereof. .

In the present invention, the upper and lower guide protrusions are formed on one side of the second movable body 20, but the present invention is not limited thereto, and the shape of the second movable body 20 may be a rod-shaped face or frame having a length in the vertical direction. Of course, it is possible to form a guide rail on the side of the face or frame.

The upper and lower guide rails, which are engaged with the upper and lower guide protrusions 24 of the second movable body 20, are moved up and down in the vertical direction, and are controlled to be selectively driven by the control means.

Here, the tube coupling portion 30 is fixed to the lower end of the east-east Shanghai 35.

The tube coupling part 30 is fixed to the lower end of the east part of Shanghai 35 and moves up and down along with the east part of Shanghai, and combines and transports the tubes 50; 51 and 52. Rectangular coupling part body 31 having two through-holes 30b formed therein, coupling auxiliary parts 32 provided at both ends of the coupling part body 31, and both side ends of the coupling part body 31 and coupling auxiliary parts 32. It is configured to include a first pusher 33 is provided in the space spaced apart) to move up and down.

Coupling body 31 is provided with at least two coupling grooves (31a) on both side edge portions of the lower bottom surface, so that the coupling protrusion (51a) of the first tube 51 to be described later to be fitted.

The coupling assistant 32 is provided with a binding member 32a on the bottom of the lower surface, and is detachably coupled to the binding portion 52a of the second tube 52 to be described later, and the binding member 32a and the binding portion 52a are provided. May be made of a magnetic material such as a magnet and a metal plate.

Here, the lower end of the coupling assistant 32 is preferably configured to be located below the lower end of the coupling unit body 31. This is so that the first tube is coupled to the coupling body, so that the first tube is easily coupled to the overlapping second tube 52 inserted from the bottom of the first tube 51, which is obvious when considering the thickness of the first tube. .

The first push rod 33 is provided to move up and down in spaces spaced apart from both side ends of the coupling portion body 31 and the coupling auxiliary portion 32, and when the tube is transferred to a desired position, it is moved downward by the control means. While moving to the binding member 32a of the coupling assistant 32 and the binding portion 52a of the second tube 52 by applying a downward force to the second tube 52 which is fastened to the lower end of the coupling assistant 32. ) To fall apart to separate the first tube 51 and the second tube (52).

In addition, the coupling portion body 31 of the tube coupling portion 30 is detachably coupled to the second pusher 40 provided with a plurality of tube guides 41 at the bottom. The upper portion of the second pusher 40 is further provided with a shanghai copper auxiliary portion 45, the shanghai copper auxiliary portion 45 is coupled to the second movable body (20; 22) to move up and down.

At this time, the tube guide 41 of the second pusher 40 is coupled to the plurality of through holes 30b of the coupling part body 31 so as to be movable in the vertical direction, so that each strip well of the first tube 51 is connected. In addition to guiding to be introduced into the position, it is further moved downward by the second pusher 40 which is moved downwards to exert downward force on the strip well of the first tube 51, and eventually The first tube 51 is separated from the coupling body 31.

The tube 50 is composed of a second tube 52 which is fitted from the lower portion of the first tube 51 and overlaps, and is conveyed to a desired position by the tube coupling portion 30, and the first tube 51 and the first tube 51 are formed. The two tubes 52 are each composed of a plurality of strip wells.

Coupling protrusions 51a are formed at both edge portions of the upper flange of the first tube 51, and a plurality of reagent spaces opened downward in a closed bottom surface of each strip well are provided for biochemical testing. Can be.

The detachable portion 52a is formed on the extended surface of the upper flange of the second tube 52, and the bottom surface of each strip well has a mesh structure, and DNA fragments or antibodies (or antigens) are spotted on the mesh structure. It can be immobilized so that immunoassays can be performed.

The bottom upper surface of the mesh structure of the second tube configured as described above is spaced apart from the bottom lower surface of the first tube by a predetermined distance, so that the inspection can be simultaneously performed on the first tube and the second tube.

The strip housing provided on the bottom surface of the multi-system is provided with a plurality of strip wells of an appropriate depth, and each strip well may carry a supernatant (serum or plasma) from which blood cells in blood are removed to a predetermined depth.

Hereinafter, a method of performing the test using the multi-system (1) which simultaneously performs the biochemical test and the immune response test according to the present invention will be described.

First, a first tube (51) comprising a plurality of strip wells, each of the plurality of strip wells is provided with a plurality of reagent spaces which are opened downward in the closed bottom surface; A second tube 52 composed of a plurality of strip wells, each bottom surface of the plurality of strip wells having a mesh structure; And a strip housing composed of a plurality of strip wells on which plasma is supported, in a multi-system for simultaneously performing a biochemical test and an immune response test.

Next, for a biochemical test, a dry indicator suitable for the test item to be measured is dispensed into each of the plurality of reagent spaces of the plurality of strip wells of the first tube 51, and then dried and fixed.

In addition, for immunoassay, DNA fragments or antibodies (antigens) are spotted and fixed to the mesh structures of the bottom surfaces of the plurality of strip wells of the second tube 52.

Next, the Shanghai East 35 is moved downward to couple the first tube 51 to the lower portion of the tube coupling portion (30).

At this time, the coupling protrusion (51a) of the first tube 51 is fitted into the coupling groove (31a) formed in the lower bottom of the coupling portion body 31 of the tube coupling portion 30, the first tube ( Each strip well of 51 is guided to a tube guide 41 protruding to the bottom bottom of the coupling body 31.

Next, the second tube 52 is coupled to the lower portion of the tube coupling portion 30 to which the first tube 51 is coupled.

At this time, the binding portion 52a of the second tube 52 is magnetically coupled to the binding member 32a at the bottom of the coupling auxiliary portion 32 of the tube coupling portion 30.

Next, the first tube 51 and the second tube 52, which are overlapped and coupled to the tube coupling part 30, are coupled to the strip housing in which plasma is supported, and reacted for 5 to 20 minutes at a temperature of 30 to 40 ° C.

The first tube 51 and the second tube 52 in which the reaction is completed are moved to the absorbance measuring unit.

Next, the first push rod 33 is moved downward to apply a downward force to the second tube 52 which is fastened to the lower end of the coupling auxiliary part 32 and the binding member 32a of the coupling auxiliary part 32. The binding part 52a of the second tube 52 is pushed apart to separate the first tube 51 and the second tube 52.

Then, the tube guide 41 is further moved downward by the second pusher 40 which is moved downward to move the second pusher 40 downward, and the tube guide 41 A downward force is applied to the strip well of the first tube 51 so that the first tube 51 is separated from the coupling body 31.

The separated first tube 51 and the second tube 52 respectively measure the absorbance at the absorbance measuring unit and convert the concentration into a biochemical test or perform an immunoassay by measuring the absorbance using a spectrophotometer.

Immunoassay is usually carried out using an antigen-antibody immune response. In general, a label is labeled on the antibody (antigen) to be tested to measure the amount of the antibody, and a spectrophotometer is used according to the labeled substance. .

As a result, biochemical tests and immune response tests can be performed simultaneously.

Although the present invention has been described with reference to the embodiments with reference to the drawings, this is only an example, those skilled in the art will understand that various modifications and equivalent embodiments are possible from this. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

1: multi-system 10: first moving object
20: second moving object 35: east of Shanghai
30: tube coupling portion 31: coupling body
32: coupling assistant 33: first push rod
40: second thumb 41; Tube guide
45: Shanghai East Assistant

Claims (14)

A mobile unit moving in the front, rear, left, and right directions; And
It is configured to include; coupled to the bottom of the mobile unit, the tube coupling portion 30 for detachably coupling at least two tubes 50,
The tube coupling portion 30,
A coupling part body 31 having an upper portion and a plurality of through holes formed at the bottom thereof to which the at least two tubes 50 are coupled; Coupling assistance portion 32 provided to be spaced apart from both ends of the coupling portion body 31; And a first push rod 33 provided in spaced spaces between both side ends of the coupling part body 31 and the coupling assistant 32 to move up and down in a vertical direction. Multi-system to perform reaction test simultaneously.
According to claim 1,
The mobile unit,
A first movable body 10 moving forward or backward from side to side along the first guide rail fixedly mounted on the upper part of the apparatus, the second guide rail being elongated in the front-rear direction on one surface;
A second moving body 20 moving forward or backward along the second guide rail of the first moving body, the upper and lower guide protrusions being provided on one side thereof; And
Simultaneously perform the biochemical test and the immune response test, characterized in that it comprises a; and the upper and lower guide rails engaged with the upper and lower guide protrusions of the second mobile body 20 to move up and down in the vertical direction; Multisystem.
The method of claim 2, wherein the multi-system,
Control means for controlling the first mobile body 10, the second mobile body 20, Shanghai East 35 and the tube coupling portion 30; and the biochemical test and immune response test characterized in that it further comprises Multi-system running at the same time.
The method of claim 2, wherein the east-east Shanghai 35,
The multi-system at the same time performing a biochemical test and immune response test, characterized in that the tube coupling portion 30 is fixed fixture.
delete According to claim 1,
The coupling part body 31 is provided with at least two coupling grooves 31a at the bottom of the biochemical test and the immune response test, characterized in that the coupling protrusion 51a of the first tube 51 is fitted. Multi-system running at the same time.
According to claim 1,
The coupling auxiliary portion 32 is provided with a binding member (32a) on the bottom bottom,
Multi-system for simultaneously performing a biochemical test and an immune response test, characterized in that the detachable portion (52a) of the second tube (52) is detachably coupled.
The method of claim 7, wherein
The binding member (32a) and the binding portion (52a) is a multi-system for simultaneously performing a biochemical test and an immune response test, characterized in that consisting of a magnetic material.
According to claim 1,
The first push rod 33,
The binding member 32a and the second tube of the coupling assistant 32 are applied downward by applying a downward force to the second tube 52 which is fastened to the lower end of the coupling assistant 32 while being moved downward by the control means. A multi-system for simultaneously performing a biochemical test and an immune response test, characterized in that the binding portion (52a) of (52) is separated.
According to claim 1,
The biochemical test and the immune response test, characterized in that the coupling unit body 31 of the tube coupling unit 30 is detachably coupled to the second pusher 40 provided with a plurality of tube guides 41 at the bottom. Multisystem running.
The method of claim 10,
The second rod 40 is further provided with a shanghai Dongbu 45,
The Shanghai East Aid 45 is a multi-system for simultaneously performing a biochemical test and an immune response test, characterized in that coupled to move up and down in the vertical direction.
The method of claim 10,
The tube guide 41 of the second pusher 40,
It is coupled to the plurality of through-holes (30b) of the coupling portion body 31 so as to be movable in the vertical direction, to guide each strip well of the first tube (51) to be put in place, as well as to move downward It is moved downward by the second pusher (40) to separate the first tube (51) from the coupling body (31) characterized in that the multi-chemistry test and the immune response test at the same time multi-system.
According to claim 1,
The tube 50 is composed of a second tube 52 sandwiched from the bottom of the first tube 51, the first tube 51 and the second tube 52 are each composed of a plurality of strip wells Multi-system to perform a biochemical test and an immune response test, characterized in that at the same time.
The method of claim 13,
A coupling protrusion 51a is formed on the upper flange of the first tube 51,
The detachable portion 52a is formed on the extended surface of the upper flange of the second tube 52, and the bottom surface of each strip well of the second tube 52 is composed of a mesh structure and Multi-system to perform immune test simultaneously.

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