KR101984377B1 - Composition for Preventing or Treating Attention Deficit Hyperactivity Disorder containing Extracts from Oriental Herb Medicines - Google Patents

Abstract

The present invention relates to a composition for the prevention or treatment of attention deficit hyperactivity disorder comprising, as an active ingredient, a mixed herbal medicine extract containing a raw material, a domesticated product, a manganese bran, Can be usefully used as an active ingredient of a composition for preventing or treating attention deficit hyperactivity disorder because it reduces hyperactivity, increases attention, and increases expression of CDK5 in the cerebral cortex in an hyperactivity disorder animal model.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing or treating attention deficit hyperactivity disorder containing an herbal extract as an active ingredient,

The present invention relates to a composition for preventing or treating attention deficit hyperactivity disorder which contains, as an active ingredient, a mixed herbal medicine extract containing a raw material, a domesticated mushroom, a moss bran,

ADHD is a disorder characterized by frequent disturbances in childhood and adolescence, characterized by persistent distraction, hyperactivity, and impulsivity. When these symptoms are left untreated, difficulties persist in various ways throughout childhood, and in some cases symptoms persist even in adolescence and adulthood. According to domestic epidemiologic survey results, the prevalence of attention deficit hyperactivity disorder is 6 ~ 8%, which is about 13% when symptoms are not serious, and it belongs to the disease with the highest prevalence among child psychiatric diseases. It is known that from the adolescence to the adulthood, attention deficit hyperactivity disorder persists from 30% to as much as 70%.

Treatment of attention deficit hyperactivity disorder is most effective with drug therapy using methylphenidate, amphetamine, dextroamphetamine, and various treatments such as learning therapy, play therapy, and social group therapy are appropriate.

It is known that herbal medicine extracts containing herbal extracts of herbal medicine, herbal medicine and herbal medicine, or only a mixture of them, have a positive effect on treatment of attention deficit hyperactivity disorder and attention and concentration. However, there is no known effect of preventing or treating attention deficit hyperactivity disorder in mixed herbal medicine extracts of Raw, Fenugreek, Morus alba, Yugunpi, Ganoderma, Chunma, and Ganoderma lucidum.

Accordingly, the present inventors have made efforts to develop a new therapeutic agent for attention deficit hyperactivity disorder. As a result, the present inventors have found that the herbal extracts of a mixture of the raw materials such as raw materials, ginseng, manganese, Confirming that it intensifies attention.

1. Korean Patent Publication No. 10-2016-0144060 2. Korean Patent Publication No. 10-2016-0000695

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating attention deficit hyperactivity disorder comprising as an active ingredient a mixed herbal medicine extract containing a raw material, a domesticated product, a manganese protein extract, And to provide a health functional food for preventing or ameliorating osteoporosis.

One aspect of the present invention provides a pharmaceutical composition for preventing or treating attention deficit hyperactivity disorder comprising, as an active ingredient, a mixed herbal medicine extract containing a raw material, a mallow, a mallow, a rhizome,

According to an embodiment of the present invention, the raw materials such as raw paper, raw moss, moss bran, corynebacterium, germano, chamai and acacia can be purchased commercially or sold or cultivated in nature, but the present invention is not limited thereto.

As used herein, the term " mixed herbal extract " means an extract isolated from a mixture of herbal medicines of raw materials, fillets, mosses, mosses,

The mixed herbal extract may be prepared by the following method. For example, raw material mixture is prepared by mixing raw materials, raw mallow, alfalfa, rhizome, cuttlefish, horsetail, and liquorice at a certain mass ratio to thoroughly immerse the herbal medicines mixture by adding extraction solvent of 10 to 30 times volume. Next, a mixed herbal medicine extract may be prepared by impregnation or warming at room temperature, and water, C ₁ to C ₄ lower alcohol, or a mixed solvent thereof may be used as the extraction solvent. The heating may be performed at 60 to 120 ° C, and may be performed once or repeatedly for 30 to 120 minutes.

On the other hand, the mixed herbal medicine extract includes an extract obtained by the conventional solvent extraction method as well as an ordinary purified method. For example, a fraction obtained through various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) May also be included in the extract of the present invention. In addition, the herbal extract may be prepared in powder form by an additional process such as vacuum distillation, lyophilization, or spray drying. Specifically, the herbal extract may be prepared in powder form through sterilization and spray drying, sterilized at a temperature of 70 to 90 ° C for 10 to 40 minutes, and then may be prepared into a powder form using a spray dryer have.

Because the herbal extract has the effect of reducing hyperactivity, increasing attention, and increasing the expression of cyclin-dependent kinase 5 in the cerebral cortex in an attention-deficit hyperactivity disorder animal model, attention deficit hyperactivity May be used as an active ingredient of a pharmaceutical composition for preventing or treating hyperactivity disorder.

The pharmaceutical composition may further comprise an appropriate carrier, excipient and diluent commonly used in the production of a pharmaceutical composition besides the mixed herbal medicine extract according to one embodiment of the present invention as an active ingredient, May be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to the method.

The pharmaceutical compositions of the present invention may be administered to a subject in a variety of routes including, but not limited to, oral, rectal or intravenous, intramuscular, subcutaneous or intracerebroventricular injections.

The term " administering " as used herein means providing a subject with the pharmaceutical composition of the present invention, alone or in combination with another treatment, in any suitable manner, and its preferred dosage will depend on the condition and weight of the patient, The type of administration, the route of administration, and the duration of administration.

The pharmaceutical composition of the present invention can be used alone or in combination with methods for the prevention or treatment of attention deficit hyperactivity disorder or using hormone therapy, chemotherapy and biological response modifiers.

Another aspect of the present invention provides a health functional food for preventing or ameliorating an attention deficit hyperactivity disorder containing, as an active ingredient, a mixed herbal medicine extract comprising raw paper, raw mackerel, mung bean bran, curcuma,

As used herein, the term " health functional food " means a food having a biological function-controlling effect such as prevention or improvement of disease, bio-defense, immunity, recovery after disease, and suppression of aging.

A mixed herbal medicine extract according to an embodiment of the present invention may be added to a health functional food for the purpose of preventing or ameliorating attention deficit hyperactivity disorder and may be added directly to health functional foods or added together with other food compositions or other food ingredients . The amount of the herbal medicine extract added may be suitably selected according to the intended use by a person skilled in the art.

The form of the above-mentioned health functional foods may include dairy products such as meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums and ice cream, various soups, beverages, tea, drinks, And combinations thereof, but are not limited thereto.

The health functional food may contain nutritional ingredients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, A preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like.

The herbal extracts of the present invention can be used to reduce hyperactivity, increase attention, and increase expression of CDK5 in the cerebral cortex in an animal model of Attention Deficit Hyperactivity Disorder have.

FIG. 1 is a graph showing the results of an open field experiment of an ADHD animal model according to mixed herbal medicine treatment. FIG.
2 is a graph showing the results of the Y-maze test of the ADHD animal model according to mixed herbal medicine treatment.
FIG. 3 is a Western result and graph showing changes in the expression of CDK5 upon treatment with mixed herbal extracts in the cerebral cortex of the ADHD animal model.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example  1: Preparation of mixed herbal medicine extract

A total of 100 g of herbal medicines were prepared by mixing the same amounts of each of seven herbal medicines, namely, raw mushroom, mushroom, mushroom, rootstock, horsetail, chestnut, and mash. To the mentioned mixture of herbal medicines, 1 L of 30% ethanol was added, left at room temperature for 30 minutes, and then at 100 DEG C for 100 minutes. Next, 300 ml of 30% ethanol was further added, and the mixture was re-mixed at the same temperature for 60 minutes. The obtained extract of the herbal medicines mixture mixture was filtered through a 50 탆 filter, and the filtrate was concentrated under reduced pressure at -60 캜 or lower and then dried to obtain 7.6 g of a final extract of the herbal medicines mixture (hereinafter referred to as mixed herbal extracts) Yield: 7.6%).

Example  2: ADHD  Identification of Behavioral and Protein Expression Changes by Mixed Herbal Drug Administration in Animal Model

2-1. ADHD  Mixed herbal medicine extract in animal model

Four male Wistar rats (WKYR) and 14 spontaneously hypertensive rats (SHR) at 6 weeks of age were prepared. Transient hypertensive rats are the most commonly used experimental animals for ADHD animal models because they exhibit hyperactivity, impulsivity, and attention deficit symptoms, which are characteristic of ADHD, compared with Wistar-Kyoto strain mice. Experimental animals were randomly sampled and treated with a control (WKY, n = 4), ADHD control (SHR, n = 4), low concentration (SHR, 30 ㎎ / And high concentration group (mixed herbal medicine extract of 100 ㎎ / kg / day in SHR, n = 5).

During the entire experimental period, the animals were allowed to freely consume solid feed and water, kept at a temperature of 24 ± 2, maintained at 60% humidity, and maintained in a 12 hour light - dark cycle controlled environment. The animals before the experiment underwent a week of adaptation period, and the mixed herbal extract of Example 1 was orally administered once a day for three weeks as a whole.

2-2. Open field  Experiment

The white rat used in the experiment showed a search behavior when exposed to a new environment, and it can be used as an index of search behavior by measuring the walking activity. Therefore, an open field test was performed to measure the hyperactivity of the ADHD animal model. The equipment used in this experiment is a 75x75x25 ㎝ black wooden box. White lines are drawn in a checkerboard pattern at intervals of 15 ㎝ on the floor, and the open field is divided into a central region and a peripheral region based on a white line drawn on the floor.

Walking activity was measured once a day before mixing herbal medicine extracts and then at 7, 14 and 21 days after administration of mixed herbal medicine extracts. For the measurement method, the animal model was placed in the open field, and the adaptation time was given for 1 minute, and then the distance traveled for 10 minutes and the travel time were measured.

FIG. 1 is a graph showing the results of an open field experiment of an ADHD animal model according to mixed herbal medicine treatment. FIG. The behavior of the ADHD control group (SHR) was more active compared to the normal control group (WKY), and it was confirmed that the hyperactivity decreased significantly at the third week after administration of the mixed herbal medicine extracts in both the low and high concentration experimental groups (* p &Lt; 0.05, ** p < 0.01, n = 4).

2-3. Y-labyrinth experiment

The Y-maze test was performed to measure the degree of attention deficit in the ADHD animal model.

The Y-maze is made up of three branches at an angle of 120 °, each branch having a length of 50 cm, a width of 10 cm, and a height of 25 cm. Each branch was defined as A, B, C, and all experimental animals were started at the same branch end to maintain the same experimental conditions. The experimental animals were placed on the tip of one branch and recorded for 8 minutes and the continuous movement was recorded. A, B, C, or B, C, A or C, A, B (A, A, B, B, B, A or C, C, C, etc.) ) Was given to 1 point, and the changing behavior of the experimental animals was calculated according to the following equation (1): &lt;

2 is a graph showing the results of the Y-maze test of the ADHD animal model according to mixed herbal medicine treatment. (ADHD) control group (SHR) was lower than that of the normal control group (WKY), and it was confirmed that the attention was strengthened significantly in both the low and high concentration test groups (* p <0.05, n = 4).

2-4. ADHD  Identification of changes in protein expression in brain tissue in animal models

In order to analyze the expression of cyclin-dependent kinase 5 (CDK5) in the brain cerebral cortex, the animals were sacrificed at the end of the experiment and the brain was extracted and proteins were isolated using methods known in the art .

A total of 30 μg of the protein was electrophoresed on 10% SDS-polyacrylamide gel, and the proteins on the gel were transferred to a nitrocellulose membrane. The membrane was then blocked with 5% skim milk overnight and contacted with anti-CDK5 (Santa Cruz Biotechnology, USA) overnight at 4 ° C. The next day, the membrane was washed three times at 10-minute intervals using TBS-T (tris-buffered saline with 0.05% tween 20) and contacted with secondary antibody (Santa Cruz Biotechnology) for 1 hour at room temperature. After 1 hour, the membrane was washed three times at 15 minute intervals using TBST, and the protein band was confirmed by chemiluminescence.

FIG. 3 is a graph showing the expression of CDK5 in the cerebral cortex of ADHD animal model according to mixed herbal medicine treatment. The expression of CDK5 was significantly increased in the low and high concentration experimental groups compared to the ADHD control (SHR) (* p <0.01, n = 2).

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (4)

The present invention relates to a pharmaceutical composition for preventing or treating attention deficit hyperactivity disorder which contains, as an active ingredient, a mixed herbal medicine extract composed of raw material, filamentous fungi, alfalfa, oriental fungus,
The mixed herbal extract
The raw materials, raw mackerel, mung bean mackerel, mung bean mackerel, mackerel mackerel, horse chestnut, and mackerel are mixed at an equivalent mass ratio to prepare a mixture of herbal medicines,
10 to 30 times volume of a 30% ethanol solvent of the above mixture was added and the mixture was immersed at room temperature for 30 minutes,
Heated to 60 to 120 DEG C and extracted for 30 to 120 minutes.
delete 2. The composition of claim 1, wherein the composition increases expression of the cyclin dependent kinase 5.
The present invention relates to a health functional food for preventing or ameliorating an attention deficit hyperactivity disorder which contains, as an active ingredient, a mixed herbal medicine extract composed of yeast extract, mulberry, alfalfa,
The mixed herbal extract
The raw materials, raw mackerel, mung bean mackerel, mung bean mackerel, mackerel mackerel, horse chestnut, and mackerel are mixed at an equivalent mass ratio to prepare a mixture of herbal medicines,
10 to 30 times volume of a 30% ethanol solvent of the above mixture was added and the mixture was immersed at room temperature for 30 minutes,
Wherein the composition is heated to 60 to 120 DEG C and then extracted for 30 to 120 minutes.

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