KR101918759B1 - Processed food using freeze-dried silkworm powder and its manufacturing method thereof - Google Patents

Processed food using freeze-dried silkworm powder and its manufacturing method thereof Download PDF

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KR101918759B1
KR101918759B1 KR1020170003390A KR20170003390A KR101918759B1 KR 101918759 B1 KR101918759 B1 KR 101918759B1 KR 1020170003390 A KR1020170003390 A KR 1020170003390A KR 20170003390 A KR20170003390 A KR 20170003390A KR 101918759 B1 KR101918759 B1 KR 101918759B1
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/44Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/424Addition of non-meat animal protein material, e.g. blood, egg, dairy products, fish; Proteins from microorganisms, yeasts or fungi
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health

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Abstract

본 발명은 5령의 누에 동결건조 분말을 이용한 가공식품 및 그 제조방법에 관한 것으로 5령의 누에를 선택하여 누에 동결건조 분말을 제조하고 상기 단계에서 제조한 누에 분말을 이용하여 조미료, 누에과자 및 누에 육류 가공식품을 제조한 다음 제조한 누에 가공식품의 기능성 및 관능평가를 실시한 결과 본 발명은 누에 분말을 이용한 가공식품 및 그 제조방법을 제공하는 효과가 있을 뿐 아니라, 영양학적, 기능적, 품질 측면에서 식감 및 기호도가 개선되고 항산화 및 혈당강하 기능성이 유지되는 신규한 누에 가공식품을 제공하고, 폐기되는 누에 재고를 방지하여 농촌 경제의 손실 및 환경오염 문제를 해결하고 나아가 누에의 이용도를 극대화할 수 있는 뛰어난 효과가 있다.The present invention relates to a processed food using freeze-dried silkworm powder of the fifth order and a method for producing the freeze-dried powder. The silkworm silkworm selected from the group consisting of five silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm silkworm As a result of functional and sensory evaluation of silkworm processed food produced after manufacturing silkworm meat processed food, the present invention not only has the effect of providing processed food using silkworm powder and a manufacturing method thereof, but also provides nutritional, functional, and quality To provide a new silkworm processed food with improved texture and preference and antioxidant and hypoglycemic functionality and to prevent the recycling of spent silkworms to solve the problems of rural economy and environmental pollution and to maximize utilization of silkworm Can have excellent effect.

Description

누에 동결건조 분말을 이용한 가공식품 및 그 제조방법{Processed food using freeze-dried silkworm powder and its manufacturing method thereof}Technical Field [0001] The present invention relates to a processed food using freeze-dried silkworm silkworm powder and a manufacturing method thereof,

본 발명은 누에 동결건조 분말을 이용한 누에 가공식품 및 그 제조방법에 관한 것이다.The present invention relates to a silkworm processed food using a silkworm freeze-dried powder and a method for producing the silkworm processed food.

누에(Bombyx mori L.)는 누에나방과에 속하는 누에나방에 유충으로 대량 사육이 가능하며 단백질 함량이 높아 고단백질 식품소재로(Cho, C. H. et al., Effect of temperature, time and pH on the extraction of protein in a chrysalis of silkworm, Korean J. Biotechnol. Bioeng., 4, P65-68, 1989), 필수 아미노산과 n-3 계열의 고도불포화 지방산 함유량이 높아 간 기능 개선이나 혈액순환 관련 건강식품 소재로 활용 가능성이 제시되고 있다(Seong, S. I. et al., Effect of metamorphois on the major hemolymph proteins of the silkworm, Arch. Insect Biochem. Physiol., 2, P91-104, 1985). 또 누에실크 유래의 sericin 단백질도 serine, aspartic acid 및 glycine으로 주로 구성되어 있어 간 보호 효과와 항산화 효과가 높은 것으로 보고되었다(Kato, N. et al., Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity, Biosci. Biotechnol. Biochem., 62, P145-147, 1998). 또한 단백질 함량이 높은 누에 분말을 Bacillus속 미생물로 발효시켜 얻은 발효누에에서 항산화 작용, 혈전용해 작용, 티로시나제 활성 저해 등의 생리활성작용이 발효 전 누에 분말보다 증가하는 것으로 보고한 바 있다(Cha, J. Y. et al., Biological activity of fermented silkworm powder, J. Life Sci., 19, P1468-1477, 2009).Silkworm ( Bombyx mori L. ) can be mass-fed as larvae in the silkworm moth, belonging to the silkworm moth, and has a high protein content and is a high protein food material (Cho, CH et al., Effect of temperature, (a) Chrysalis of silkworm, Korean J. Biotechnol. Bioeng., 4, P65-68, 1989), high content of essential amino acids and n-3 polyunsaturated fatty acids, (Seong, SI et al., Effect of metamorphism on the major hemolymph proteins of the silkworm, Arch. Insect Biochem. Physiol., 2, P91-104, 1985). In addition, sericin protein from silkworm silk is mainly composed of serine, aspartic acid and glycine, and thus it has been reported that it has high liver protection effect and antioxidant effect (Kato, N. et al., Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity , Biosci. Biotechnol. Biochem., 62, P145-147, 1998). In addition, it has been reported that physiological activities such as antioxidation, thrombolytic action and inhibition of tyrosinase activity are increased in fermented silkworm obtained by fermenting silkworm powder with high protein content with Bacillus sp. et al., Biological activity of fermented silkworm powder, J. Life Sci., 19, P1468-1477, 2009).

짠맛은 식품의 관능적인 특성을 상승시켜주는데 대표적인 짠맛을 가지는 물질은 나트륨(Na)과 염소(Cl)가 결합된 소금(염화나트륨, NaCl)이다. 소금에서 나트륨은 인체의 적절한 수분균형을 유지시켜 주며, 혈압 및 신경의 흥분을 조절하여 근육의 수축과 영양소의 이동에 관여하며 음식물의 보존성 향상, 음식물의 물성개선 등의 중요한 역할을 한다. 그러나 높은 수준의 나트륨 섭취는 고혈압, 뇌졸중 및 심혈관계 질환의 발생 위험을 증가시키는 문제점이 있는데(Strazzullo P. et al., Salt intake, stroke, and cardiovascular disease: meta-analysis of prospective studies, BJM 339, b4567, 2009), 식품의약품안전청에서는 2020년까지 나트륨 섭취량을 20% 이상 감소시키는 것을 목표로 하여 저나트륨 급식확대, 외식 시범특구 확대, 저나트륨 가공식품 생산 확대, 저나트륨 가정용 레시피 보급 및 소비자 캠페인 실시 등 5개 분야에 대한 정책을 추진하고 있으며 나트륨 섭취감소를 위한 방법으로는 소금 입자의 크기나 형태를 변화시키는 등의 물리적 방법, 나트륨을 다른 무기염으로 대체하거나 천연 대체제를 사용하여 소금의 짠맛을 증진시키는 소재를 개발하는 등의 화학적인 방법 및 후각, 인지정도 개선 연구 등의 생물학적인 접근방식으로 맛에 대한 감지에 영향을 미치는 방법 등 다양한 방법들이 시도되고 있다. 저염을 위한 대체물질 현황을 보면, 효모추출물(46.7%), 천일염(28.4%), MSG(23.2%), 염화칼륨(15.5%)의 순으로 나타나고 있다. 효모추출물은 나트륨 대체재로 사용되거나 혹은 지미를 부여하기 위한 용도로 사용되고 있으며 효모추출물 및 MSG의 경우에는 저나트륨 함량임에도 좋은 맛을 유지하기 위한 목적으로 첨가되나 저염효과는 아직까지 미흡한 수준이다. 그리고 염화칼륨의 경우 소스 및 양념류, 육류, 식사대용식, 제과, 유제품 등에서 폭넓게 사용되고 있으나 다량 사용하게 되면 특유의 금속성 쓴맛으로 인하여 음식자체의 맛이 나빠지며 장기적으로 사용할 경우 신장기능 및 심장박동 등에 질환의 위험이 높아질 수 있다. 이에 저염화 및 관능적으로 우수한 소금 대체소재 또는 염미 증강물질을 개발하는 것이 절실히 요구된다. The salty taste enhances the sensual characteristics of food. Salty taste is a salt (sodium chloride, NaCl) combined with sodium (Na) and chlorine (Cl). In salt, sodium maintains the proper water balance of the body, regulates blood pressure and nerve excitement, and plays a role in the contraction of muscles and the movement of nutrients, improving the preservation of food and improving the physical properties of food. However, high levels of sodium intake have been associated with increased risk of developing hypertension, stroke, and cardiovascular disease (Strazzullo P. et al., Meta-analysis of prospective studies, BJM 339, b4567, 2009), KFDA is aiming to reduce sodium intake by 20% or more by 2020, expanding low-sodium feed, expanding eatery special zone, expanding production of low-sodium processed food, , And physical methods such as changing the size and shape of the salt particles. The sodium salt can be replaced by other inorganic salts or by using a natural substitute to enhance the saltiness of the salt. Biological methods such as the development of materials to be developed, such as chemical methods, olfactory and cognitive improvement studies And a method of influencing the sensation of taste are tried in various ways. Alternative substances for low salt are listed in order of yeast extract (46.7%), sun salt (28.4%), MSG (23.2%) and potassium chloride (15.5%). Yeast extract is used as a substitute for sodium or to give Jimmy. Yeast extract and MSG are added for the purpose of maintaining good taste even in low sodium content, but the low salt effect is still insufficient. In addition, potassium chloride is widely used in sauces, seasonings, meals, substitute meals, confectionery, and dairy products. However, when used in large amounts, the taste of the food itself deteriorates due to the unique metallic bitter taste. Can be increased. Therefore, there is a desperate need to develop a salt substitute material or a salt enhancement material which is excellent in low chloride and sensory properties.

한편, 가공식품은 농산물, 축산물 및 수산물을 보다 맛있고 먹기 편하게 변형시키는 동시에 저장성이 높인 식품을 말하며 과자, 통조림, 잼 등이 그 예이다.현재 국내에서는 지역의 특산물을 활용한 가공식품 개발이 활발히 이루어지고 있는데 그 이유는 특정지역의 생산품을 판매하는데 그치지 않고 지역을 홍보하는 효과와 더불어 제품 제고가 생기지 않도록 하고 지역경제 활성화에 크게 기여할 수 있기 때문이다. 최근 국내에서 활발히 제조 및 유통되고 있는 지역특산물로는 인천과 경남 통영시의 수산물을 활용한 김, 액젓, 젓갈, 엑기스 등과 충북진천의 특산물인 쌀, 흑미, 찹쌀과 같은 곡류를 사용한 된장, 간장, 청국장 및 고추장, 제주도의 용과, 감귤 등을 활용한 초콜릿, 주스, 쿠키 등이 있다(Kim, E. M. Actual condition of regional products management on internet shopping mall, The Korea Contents Association, 10, 56-60, 2012). 그러나 충북 영동군의 누에는 누에가루, 누에환, 기능성 식품으로만 유통되고 있어 제품 제고가 생기는 한계점이 있다.In the meantime, processed foods are foods that are more delicious and easier to eat, and that have higher storage stability, such as sweets, canned foods, and jams. Currently, domestic processed foods are actively developed using local specialties The reason for this is that it does not just sell products in a certain area but also promotes the region, so that products can not be raised and contributes greatly to revitalization of the local economy. Local specialties that have been actively manufactured and circulated in Korea include Kimchi, fish sauce, salted fish, extract, which are made by using fishery products of Incheon and Gyeongnam Tongyeong, and miso, which is a special product of Chungbuk Jincheon, such as rice, black rice, glutinous rice, And chocolate, juice, and cookies that utilize citrus fruits such as kochujang, jeju-do, and citrus fruits (Kim, EM, 2003, The Korea Contents Association, 10, 56-60, 2012). However, the silkworms in Yeongdong-gun, Chungbuk Province are distributed only with silkworm powder, silkworm, and functional foods.

누에에 대한 선행기술로는 누에번데기 기름을 유효성분으로 함유하는 건강식품이 대한민국 등록특허 제10-0715117호에 개시된 바 있고 누에 분말 추출물을 함유하는 기능성 건강보조식품 및 그 제조방법이 대한민국 등록특허 제10-0564202호에 개시된 바 있다. 또한, 발효 누에 분말의 제조방법 및 발효 누에 분말을 포함하는 고지혈증 및 지방간 예방 또는 치료용 조성물이 대한민국 등록특허 제10-1246266호에 개시된 바 있다. 그러나 상기문헌 어디에도 누에 동결건조 분말을 이용한 조미료 및 가공식품에 대하여는 개시되거나 암시된 바 없다.As a prior art for silkworm, a health food containing an effective amount of silkworm pupa oil is disclosed in Korean Patent No. 10-0715117, and a functional health supplement containing silkworm powder extract and its preparation method are disclosed in Korea Patent No. 10-0564202. Further, a composition for preventing or treating hyperlipidemia and fatty liver comprising a fermented silkworm powder and a fermented silkworm powder is disclosed in Korean Patent No. 10-1246266. However, none of the above documents disclose or suggest any seasonings and processed foods using silkworm powder.

따라서, 본 발명의 목적은 누에 동결건조 분말을 이용한 신규한 가공식품을 제공하는데 있다.Accordingly, it is an object of the present invention to provide a novel processed food using the silkworm freeze-dried powder.

본 발명의 다른 목적은 아미노산, 당 및 단백질 섬유가 극대화된 5령의 누에의 성분파괴를 최소화한 동결건조 분말을 이용하여 신규한 조미료, 과자 및 육가공식품 및 그 제조방법을 제공하는 데 있다.Another object of the present invention is to provide a novel seasoning, confectionery, and meat product using the freeze-dried powder which minimizes the destruction of the component of the silkworm larva of 5th stage in which amino acid, sugar and protein fiber are maximized.

본 발명의 상기 목적은 누에 동결건조 분말을 제조하는 단계와; 상기 단계에서 얻은 누에 분말을 이용하여 조미료를 제조하는 단계와; 이와 달리 상기 단계에서 얻은 누에 분말을 이용하여 튀김과자를 제조하는 단계와; 또 이와 달리 상기 단계에서 얻은 누에 분말을 이용하여 육류 가공식품을 제조하는 단계와; 상기 단계에서 제조한 신규한 조미료, 과자 조성물, 육류 가공식품의 관능을 시중판매 제품과 비교 평가하는 단계와; 항산화 활성을 평가하는 단계와; 혈당강하 활성을 확인하는 단계를 통하여 달성하였다.
The above object of the present invention is achieved by a method for producing silkworm freeze-dried powder, Preparing a seasoning using the silkworm powder obtained in the above step; Alternatively, the step of preparing fried confectionery using the silkworm powder obtained in the step (a) Alternatively, the method may further comprise the steps of: preparing meat processed food using the silkworm powder obtained in the above step; Comparing the sensory evaluation of the novel seasoning, the cookie composition, and the meat processed food prepared in the above step with a marketed product; Evaluating antioxidant activity; And confirming the hypoglycemic activity.

본 발명은 누에 동결건조 분말을 이용한 다양한 가공식품 및 그 제조방법을 제공하는 효과가 있을 뿐 아니라, 영양학적, 기능적, 품질 측면에서 누에 가공식품의 가치를 향상시키고 누에를 활용하여 누에 재고가 생기지 않아 농촌 경제의 손실 및 환경오염 문제 해결과 나아가 누에의 이용도를 극대화할 수 있는 뛰어난 효과가 있다. The present invention is not only effective in providing a variety of processed foods using the silkworm freeze-dried powder and a method for producing the same, but also provides a method of improving the value of silkworm processed food in nutritional, functional, and quality aspects, There is an excellent effect of solving the problems of the rural economy and environmental pollution and maximizing the utilization of the silkworm.

도 1은 본 발명에 사용한 누에를 나타낸 사진도이다.
도 2는 본 발명에 사용한 누에 동결건조 분말을 나타낸 사진도이다.
도 3은 본 발명 누에 동결건조 분말을 이용한 조미료 제조방법을 도시한 블록 다이어그램이다.
도 4는 본 발명 누에 동결건조 분말을 이용한 과자 제조방법을 도시한 블록 다이어그램이다.
도 5은 본 발명 누에 동결건조 분말을 이용한 육류 가공식품의 제조방법을 도시한 블록 다이어그램이다.
1 is a photograph showing a silkworm used in the present invention.
2 is a photograph showing a silkworm freeze-dried powder used in the present invention.
3 is a block diagram showing a method of manufacturing a seasoning using the silkworm silk powder according to the present invention.
FIG. 4 is a block diagram showing a method of manufacturing cookies using the silkworm freeze-dried powder of the present invention.
Fig. 5 is a block diagram showing a method for producing a meat-processed food using the silkworm freeze-dried powder according to the present invention.

본 발명에서 사육한 5령 누에는 통채로 튀기거나 굽거나 삶아서 손쉽게 섭취할 수 있다.The fifth silkworms raised in the present invention can be easily ingested by frying, bending, or boiling them in a pan.

본 발명에 따르면 그러나, 신규한 누에 조미료를 제조하기 위한 재료 배합비는 조미료 조성물 총 중량대비 5령의 누에 동결건조 분말 20~45중량%를 유효성분으로 함유하는 것을 특징으로 하고, 이에 다시마 10~15중량%, 양파 10~15중량%, 파 5~7중량%, 마늘 5~7중량%, 멸치 5~10중량%, 새우 5~10중량% 및 표고버섯 15~16중량%를 포함하는 범위 내에서 사용하는 것이 가장 바람직하였다.According to the present invention, however, the mixing ratio of ingredients for preparing the novel silkworm seasonings is characterized by containing 20 to 45% by weight of freeze-dried silkworm powder of five years old as the active ingredient, based on the total weight of the seasoning composition, A range of 5 to 10 weight% of onion, 5 to 7 weight% of onion, 5 to 7 weight% of garlic, 5 to 10 weight% of anchovy, 5 to 10 weight% of shrimp and 15 to 16 weight% of shiitake mushroom Was most preferred.

또, 본 발명 신규한 누에 과자를 제조하기 위한 재료 배합비는 과자 조성물 총 중량대비 타피오카 가루 65~80중량%에 5령의 누에 동결건조 분말 7~15중량%를 유효성분으로 함유하는 것을 특징으로 하고 설탕 4~6중량%, 소금 4~6중량%, 탄산수소나트륨 2~3중량%, 마늘가루 2~3중량% 및 구연산 1~2중량%를 함유하는 것이 가장 바람직하였다.The mixing ratio of ingredients for preparing the novel silkworm cake of the present invention is characterized in that it comprises 65 to 80% by weight of tapioca powder and 7 to 15% by weight of freeze-dried silkworm powder of 5 days as an effective ingredient Most preferably contains 4 to 6% by weight of sugar, 4 to 6% by weight of salt, 2 to 3% by weight of sodium hydrogencarbonate, 2 to 3% by weight of garlic powder and 1 to 2% by weight of citric acid.

또한, 본 발명 신규한 누에 육류 가공식품을 제조하기 위한 재료 배합비는 육류가공식품 조성물 총 중량대비 다진 고기 20~50중량%에 5령의 누에 동결건조 분말 15~20중량%를 유효성분으로 함유하고, 이에 정제수 15~20중량%, 다진 양파 9~15중량%, 다진 마늘 5~10중량%, 난백분 1~3중량%, 간장 2~5중량%, 청주 1~3중량%, 후추 1~2중량% 및 소금 1~2중량%를 함유하는 범위 내에서 사용하는 것이 가장 바람직하였다.In addition, the mixing ratio of ingredients for preparing the novel silkworm meat processed food according to the present invention is such that 20 to 50% by weight of the minced meat is contained as an effective ingredient in 15 to 20% by weight of the silkworm silk powder 15 to 20 wt% of purified water, 9 to 15 wt% of chopped onion, 5 to 10 wt% of chopped garlic, 1 to 3 wt% of egg white, 2 to 5 wt% of soy sauce, 1 to 3 wt% of sake, By weight and 1 to 2% by weight of salt.

본 발명에서 가공식품 제조시 사용한 식품재료는 K마트에서 구매하여 사용하였다.In the present invention, the food materials used in the manufacture of processed foods were purchased from K-Mart and used.

이하, 본 발명을 실시예에 따라 더욱 상세하게 설명한다. 그러나 하기 실시예는 본 발명을 예시하기 위한 것에 불과하므로 본 발명의 권리범위를 한정하는 것으로 의도되지는 않는다.
Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples are merely illustrative of the present invention and are not intended to limit the scope of the present invention.

<실시예 1> 누에 동결건조 분말 제조&Lt; Example 1 > Preparation of silkworm freeze-dried powder

누에를 12 ~ 15일간 사육하여 가장 바람직하게는 5령의 누에로 키운 다음 정제수에 세척하고 급속냉동하였다. 상기 단계에서 얻은 5령 누에 냉동물을 동결건조하고 제분기에 제분하여 아미노산, 당 및 단백섬유질이 극대화된 누에 동결건조 분말을 제조하였다(도 1).
The silkworms were raised for 12 to 15 days, most preferably for 5 years, and then washed in purified water and rapidly frozen. The 5th-old silkworm frozen product obtained in the above step was lyophilized and milled in a quenching mill to prepare silkworm freeze-dried powder having the maximum amino acid, sugar and protein fiber (FIG. 1).

<< 실시예Example 2> 본 발명 누에 조미료 제조 2> Production of silkworm seasoning according to the present invention

실시예1의 누에 동결건조 분말, 다시마, 마늘, 파, 양파, 멸치, 새우, 표고버섯을 [표 1]과 같은 비율로 혼합하고 혼합물 총 중량대비 5 ~ 10배(w/w)의 정제수를 첨가하여 가열추출한 다음 추출물을 2배 이상 농축하였다. 상기 단계에서 얻은 농축물에 농축물 총 중량대비 소금을 가장 바람직하게는 1중량% 첨가하고 혼합하여 본 발명 누에 조미료를 제조하였다.
The silkworm freeze-dried powder of Example 1, kelp, garlic, onion, onion, anchovy, shrimp, and shiitake were mixed in the same ratio as in Table 1, and 5 to 10 times (w / w) And the extract was concentrated twice or more. To the concentrate obtained in the above step, salt is most preferably added in an amount of 1% by weight based on the total weight of the concentrate and mixed to prepare the silkworm seasoning of the present invention.

본 발명 누에 조미료의 배합비The blending ratio of the silkworm seasoning of the present invention 재료material 배합비(중량%)Compounding ratio (% by weight) 본 발명 누에 동결건조 분말The present silkworm freeze-dried powder 20~4520 ~ 45 다시마Kelp 10~1510 to 15 양파onion 10~1510 to 15 wave 5~75 ~ 7 마늘garlic 5~75 ~ 7 멸치Anchovy 5~105 to 10 새우shrimp 5~105 to 10 표고버섯Shiitake mushrooms 15~1615 ~ 16

<< 비교예Comparative Example 1> 본 발명 누에 조미료와 시판 조미료의 관능검사 1> Sensory evaluation of silkworm seasoning and commercial seasoning

본 발명 실시예 2에 따라 제조한 누에 조미료 및 타사의 조미료에 정제수를 첨가하여 10배(w/v) 희석한 후 관능검사를 실시하였다. 관능검사는 일반 20~30대 남녀 각각 10명씩을 요원으로 선정하고 짠맛, 단맛, 신맛, 쓴맛, 전체적인 기호도에 대하여 5점 채점법을 이용하여 매우 강하다(매우 좋다) : 5점, 보통이다 : 3점, 매우 약하다(매우 나쁘다) : 1점으로 나타내었다.The silkworm seasoning prepared according to Example 2 of the present invention and other seasonings were added with purified water and diluted 10 times (w / v), and then subjected to sensory evaluation. The sensory test was conducted by using the 5-point scoring method for the salty, sweet, sour, bitter, and overall preference (very good): 5 points, average: 3 points , Very weak (very bad): 1 point.

실험결과, [표 2]에 나타낸 바와 같이 전체적인 기호도가 일반 시판 조미료와 차이가 나타나지 않거나 더 높은 것을 확인하였다.
As a result of the experiment, it was confirmed that the overall preference as shown in [Table 2] was not different or higher than that of the common commercial seasoning.

본 발명 누에 조미료와 타사 조미료 제품의 관능적 특성The present invention relates to sensory properties of silkworm seasonings and other seasoning products 짠맛Salty taste 단맛sweetness 신맛Sour taste 쓴맛bitter 전체적인 기호도Overall likelihood 본 발명
누에 조미료
Invention
Silkworm seasoning
2.43±1.002.43 ± 1.00 3.49±1.093.49 ± 1.09 2.88±1.362.88 ± 1.36 1.21±0.881.21 0.88 4.24±0.274.24 ± 0.27
C사의 조미료C seasoning 3.89±1.173.89 ± 1.17 3.11±1.123.11 ± 1.12 2.89±0.342.89 + - 0.34 3.11±0.053.11 ± 0.05 4.22±0.874.22 + 0.87 O사의 조미료O company's seasoning 2.44±1.242.44 ± 1.24 3.25±1.503.25 ± 1.50 2.79±0.772.79 ± 0.77 3.01±0.133.01 ± 0.13 3.17±1.203.17 ± 1.20 K사의 조미료K seasoning 3.20±0.873.20 ± 0.87 2.78±0.622.78 ± 0.62 3.11±0.343.11 + - 0.34 3.24±0.053.24 ± 0.05 4.09±0.454.09 ± 0.45

<< 실시예Example 3> 본 발명 누에 과자 제조 3> Manufacture of silkworm cake

본 발명 누에 과자 제조시 바삭바삭한 식감과 전체적인 기호도를 극대화하기 위하여 가장 바람직하기로는 타피오카 가루 65~80중량%에 누에 동결건조 분말 7~15중량%로 조절하고 나머지 재료성분 설탕, 정제소금, 탄산수소나트륨, 마늘가루 및 구연산을 [표 3]과 같은 배합비로 혼합한 다음 정제수를 첨가하여 반죽하고 가로세로 0.5 cm의 크기로 절단하였다. 상기 단계에서 절단한 반죽 조성물을 가장 바람직하게는 170 ~ 180℃에서 90~100초 동안 유탕에 튀겨 본 발명 누에 과자를 제조하였다.
In order to maximize crunchy texture and overall acceptability in the manufacture of silkworm cake according to the present invention, it is most preferable to adjust the silkworm powder to 65 to 80% by weight and 7 to 15% by weight of the silkworm silk powder, Sodium, garlic powder, and citric acid were mixed at a blending ratio as shown in Table 3, followed by addition of purified water, kneading, and cutting to a size of 0.5 cm in length and width. The dough composition cut in the above step is most preferably fried in a hot water at 170 to 180 DEG C for 90 to 100 seconds to prepare the present invention silkworm cake.

본 발명 누에 과자 반죽 배합비The present invention relates to a dough paste composition 재료material 배합비(중량%)Compounding ratio (% by weight) 타피오카 가루Tapioca powder 65~8065 to 80 본 발명 누에 분말The silkworm powder 7~157 ~ 15 설탕Sugar 4~64 to 6 소금Salt 4~64 to 6 탄산수소나트륨Sodium hydrogencarbonate 2~32 to 3 마늘가루Garlic powder 2~32 to 3 구연산Citric acid 1~21-2

<< 비교예Comparative Example 2> 본 발명 누에 과자와 시판 과자의 관능검사 2> Sensory evaluation of silkworms and commercial sweets

본 발명 실시예 3에 따라 제조한 누에 과자와 R사와 N사의 새우과자와 비교하여 관능검사를 실시하였다. 관능검사는 일반 20~30대 남녀 각각 10명씩을 요원으로 선정하고 짠맛, 단맛, 식감, 전체적인 기호도에 대하여 5점 채점법을 이용하여 매우 강하다(매우 좋다) : 5점, 보통이다 : 3점, 매우 약하다(매우 나쁘다) : 1점으로 나타내었다.The sensory evaluation was carried out by comparing the silkworm cake prepared according to Example 3 of the present invention with the shrimp confectionery of R and N. The sensory evaluation was conducted by using a 5-point scoring method for salty taste, sweet taste, texture, overall taste, and very strong (very good): 5 points, average: 3 points, Weak (very bad): indicated by 1 point.

실험결과, [표 4]에 나타낸 바와 같이 [표3]의 배합비의 구성에 따르는 경우 바삭바삭한 식감과 전체적인 기호도에 있어서 일반 시판 R사 및 N사의 새우과자와 큰 차이가 나타나지 않는 것을 확인되었고 동시에 후술하는 바와 같이 누에 가루가 본래 가지고 있는 항산화효과와 혈당강하 효과를 유지할 수 있었음을 확인되었고 동시에 후술하는 바와 같이 누에가루가 본래 가지고 있는 항산화 효과와 혈당강하 효과를 유지할 수 있었음을 확인하였다(표6 및 표7).
As shown in Table 4, it was confirmed that there was no significant difference in crisp texture and overall acceptability between the commercially available R and N shrimp confections according to the composition ratio of [Table 3] It was confirmed that the silkworm powder was able to maintain its original antioxidative and hypoglycemic effects, and at the same time, it was confirmed that the silkworm powder retained its original antioxidative and hypoglycemic effects (see Table 6 and Table 7) Table 7).

본 발명 누에 과자와 타사 과자 제품의 관능적 특성Sensory characteristics of snack confectionery and other confectionery products according to the present invention 짠맛Salty taste 단맛sweetness 식감Texture 전체적인 기호도Overall likelihood 본 발명 누에 과자Invention invention 2.43±1.002.43 ± 1.00 2.78±0.952.78 0.95 4.52±0.304.52 + - 0.30 4.31±0.224.31 ± 0.22 R사의 새우과자R company shrimp confection 3.20±1.133.20 ± 1.13 3.05±1.003.05 ± 1.00 3.27±0.933.27 ± 0.93 4.22±0.874.22 + 0.87 N사의 새우과자N Shrimp Sweets 3.45±0.873.45 ± 0.87 2.80±1.722.80 ± 1.72 4.07±1.074.07 ± 1.07 4.17±1.204.17 ± 1.20

<< 실시예Example 4> 본 발명 누에 육류 가공식품 제조 4> Manufacture of noodle meat processed food

본 발명 누에 육류 가공식품 제조시 기호도 유지하면서 항산화 및 혈당강하 활성을 유지하기에 가장 바람직하기로는 다진 소고기 20~50중량%에 5령의 누에 동결건조 분말, 정제수, 다진 양파, 다진 마늘, 난백분, 간장, 청주, 후추 및 소금을 [표 5]의 배합비로 혼합하고 2~10℃에서 24시간 동안 숙성시켜 반죽을 만든 다음 스테이크 또는 볼 형태로 성형하고 가장 바람직하게는 130~140℃에서 90~100초 동안 유탕에 튀겨서 본 발명 누에 육류 가공식품을 제조하였다. In order to maintain antioxidant and hypoglycemic activity while maintaining preference in the production of meat processed food of the present invention, 5 to 20% by weight of freeze-dried silkworm powder, purified water, chopped onion, chopped garlic, egg white, The mixture of soy sauce, sake, black pepper and salt is mixed at the mixing ratio shown in Table 5, aged at 2 to 10 ° C for 24 hours to form a dough, and then molded into a steak or ball shape, most preferably at 130 to 140 ° C, And fried in a hot water for 2 seconds to prepare the meat processed food of the present invention.

본 발명에서 누에 육류 가공식품 제조시 사용하는 고기는 우육 대신 다진 소고기를 주재로한 본 발명 배합비에 따른 육류 가공식품의 항산화 및 혈당 강하 활성 건강 기능성 담보에 필요한 효과를 나타냈다(표6 및 표7 참조). 그러나 돈육, 계육, 어육 중에서 선택되는 어느 하나의 육류로 대체하여도 무방하다.
In the present invention, the meat used in the production of snack meat processed food showed the necessary effects in antioxidant and hypoglycemic active health functional collateral of meat processed food according to the mixing ratio of the present invention based on minced beef instead of beef (see Tables 6 and 7) ). However, it can be replaced with any meat selected from pork, chicken meat, and fish meat.

본 발명 누에 육류 가공식품 배합비Invention of the present invention 재료material 배합비(중량%)Compounding ratio (% by weight) 다진 소고기Minced beef 20~5020 to 50 본 발명 누에 분말The silkworm powder 15~2015-20 정제수Purified water 15~2015-20 다진 양파Chopped onion 9~159 ~ 15 다진 마늘chopped garlic 5~105 to 10 난백분Egg white 1~31-3 간장Soy sauce 2~52 to 5 청주Rice wine 1~31-3 후추pepper 1~21-2 소금Salt 1~21-2

<< 실험예Experimental Example 1> 본 발명 누에 가공식품의 항산화 활성 측정 1> Antioxidant activity measurement of snack food of the present invention

(1) 누에 동결건조 분말 및 누에 가공식품 수 추출물 제조(1) Production of water extract of freeze-dried silkworm powder and processed silkworm food

본 발명 실시예에 따른 누에 동결건조 분말 및 각각의 누에 가공식품을 homogenizer로 분쇄 및 균질화 한 다음 분쇄물 중량의 10배(w/w)의 물을 첨가하여 60~90℃에서 열수 추출한 후 여과하였다. 상기 추출과정을 2회 반복 실시하여 얻은 누에 가공식품 수 추출물을 감압 농축하고 증류수에 100, 50, 25 mg/ml로 용해시켜 본 발명 공시재료로 사용하였다.
The silkworm freeze-dried powder according to the present invention and each silkworm processed food were pulverized and homogenized with a homogenizer, and then water was added at a rate of 10 times (w / w) of the weight of the pulverized material, followed by hot water extraction at 60 to 90 ° C and filtration . The water extract of silkworm processed food obtained by repeating the above extraction process twice was concentrated under reduced pressure and dissolved in distilled water at 100, 50, and 25 mg / ml, and used as the present disclosure material.

(2) DPPH(2) DPPH

1,1-diphenyl-2-picryl-hydrazyl(DPPH, Wako, Tokyo, Japan) 6 mg을 Ethanol 50 mL에 녹인 후 동량의 증류수를 혼합하여 DPPH 반응액을 제조하였다. 본 발명 누에 분말 및 누에 가공식품 수 추출물 0.5 mL에 상기 DPPH 반응액 5 mL를 넣어 잘 혼합한 후 암실에서 15분 간 방치한 다음, 517 nm에서 흡광도를 측정하였다. 결과 값은 [수학식 1] 공식에 따라 산출하였다.
6 mg of 1,1-diphenyl-2-picryl-hydrazyl (DPPH, Wako, Tokyo, Japan) was dissolved in 50 mL of ethanol and mixed with the same amount of distilled water to prepare DPPH reaction solution. 5 mL of the DPPH reaction solution was added to 0.5 mL of the silkworm powder of the present invention and the silkworm processed water extract, and the mixture was allowed to stand for 15 minutes in a dark room and then absorbed at 517 nm. The resultant value was calculated according to the formula (1).

[[ 수학식Equation 1] One]

Figure 112017002841232-pat00001

Figure 112017002841232-pat00001

실험결과, [표 6]에서 나타낸 바와 같이 본 발명 누에 조미료의 50 mg/mL 농도에서 90% 이상의 높은 항산화 활성을 확인하였으며 누에과자 및 누에 육류 가공식품에서도 항산화 활성이 있는 것을 확인하였다.
As shown in Table 6, the antioxidative activity of the present invention was 90% or higher at a concentration of 50 mg / mL of the silkworm seasoning of the present invention, and it was confirmed that the antioxidative activity was also found in the silkworm cake and silkworm processed food.

본 발명 누에 동결건조 분말 및 누에 가공식품의 항산화효과The antioxidative effect of the freeze-dried powder of silkworm and the processed food of silkworm 농도 (mg/mL)Concentration (mg / mL) 누에 동결건조분말Silkworm freeze-dried powder 누에 조미료Silkworm seasoning 누에 과자Silkworm cake 누에 육류 가공식품Noodle Meat Processed Food 2525 25.5625.56 40.6540.65 5.275.27 10.3010.30 5050 48.8148.81 97.5597.55 9.979.97 22.4122.41 100100 80.2480.24 98.1298.12 15.7815.78 30.7530.75

<< 실험예Experimental Example 2> 본 발명 누에 가공식품의 혈당강하 효과 확인 2> Confirmation of blood glucose lowering effect of processed food of silkworm silkworm

(1) 누에 동결건조 분말 및 누에 가공식품 70% 메탄올 추출물 제조(1) Manufacture of 70% methanol extract of silkworm freeze-dried powder and silkworm processed food

본 발명 실시예에 따른 누에 동결건조 분말 및 각각의 누에 가공식품을 homogenizer로 분쇄 및 균질화 한 다음 분쇄물 중량의 10배(w/w)의 70% 메탄올을 첨가하여 60℃에서 추출한 후 여과하였다. 상기 추출과정을 2회 반복 실시하여 얻은 누에 가공식품 70%메탄올 추출물을 동결건조하였다.
The silkworm freeze-dried powder according to the present invention and each silkworm processed food were pulverized and homogenized with a homogenizer, and then 70% methanol of 10 times (w / w) the weight of the pulverized material was added and extracted at 60 ° C and then filtered. The above extraction process was repeated twice, and the 70% methanol extract of the silkworm processed food was lyophilized.

(2) 혈당강하 효과 확인(2) Confirming blood glucose lowering effect

6주령 마우스(mouse)를 12시간 절식시켜서 대조군은 maltose 500 mg/kg만을 투여하였고, 실험군은 추출 시료와 maltose 500 mg/kg씩을 실험동물에 동시투여한 후, one touch Ⅱ(Johnson&Johnson, USA)를 사용하여 마우스 꼬리 정맥에서 혈액을 채취하고 식후 45분의 혈당을 측정하여 [수학식 2]에 따라 결과값을 계산하였다.
6-week-old mice were fasted for 12 hours. The control group was administered only 500 mg / kg of maltose. In the experimental group, 500 mg / kg of maltose and extract samples were simultaneously administered to the experimental animals and one touch Ⅱ (Johnson & Johnson, USA) Blood was collected from the mouse tail vein and blood glucose was measured for 45 minutes after the meal, and the result was calculated according to the following formula (2).

[[ 수학식Equation 2] 2]

Figure 112017002841232-pat00002

Figure 112017002841232-pat00002

실험결과, [표 7]에서 나타낸 바와 같이 누에 동결건조 분말이 70% 이상의 활성을 보였으며 누에 조미료 40%, 누에 과자 13%, 누에 육류 가공품 19% 이상의 활성을 나타내는 것을 확인하였다.
As shown in Table 7, the silkworm freeze-dried powder showed more than 70% activity and showed activity of silkworm seasoning 40%, silkworm cake 13% and silkworm processed product 19% or more.

본 발명 누에 동결건조 분말 및 누에 가공식품의 혈당강하 효과Effect of the present invention on the blood glucose lowering effect of freeze-dried powder and silkworm processed food 혈당강하 효과(%)Blood glucose lowering effect (%) 누에 동결건조 분말Silkworm freeze-dried powder 누에 조미료Silkworm seasoning 누에 과자Silkworm cake 누에 육류 가공식품Noodle Meat Processed Food 72.1772.17 41.7341.73 13.0713.07 19.8919.89

본 발명은 영양학적, 기능적, 품질 측면에서 신규한 누에 가공식품을 제공하는 뛰어난 효과가 있으며 누에의 이용도를 극대화할 수 있으므로 가공식품 산업상 매우 유용한 발명인 것이다.The present invention has an excellent effect of providing novel silkworm processed foods in nutritional, functional and quality aspects and is an extremely useful invention in the processed food industry because it can maximize the utilization of silkworms.

Claims (2)

타피오카 가루 65~80중량%에 5령의 누에 동결건조 분말 7~15중량%를 포함하고 나머지는 설탕 4~6중량%, 소금 4~6중량%, 탄산수소나트륨 2~3중량%, 마늘가루 2~3중량% 및 구연산 1~2중량%가 배합된 누에 분말의 혼합 조성물을 제조하는 단계와; 상기 단계에서 얻은 누에 분말 혼합 조성물에 정제수를 첨가하여 반죽하고 절단하는 단계와; 상기 단계에서 얻은 절단물을 170 ~ 180℃에서 90~100초간 유탕에 튀기는 것이 특징인 누에 과자의 제조방법.
Dried powder of 7 to 15 wt% of silkworm powder is contained in 65 to 80 wt% of tapioca powder, 4 to 6 wt% of sugar, 4 to 6 wt% of salt, 2 to 3 wt% of sodium hydrogencarbonate, 2 to 3% by weight of citric acid and 1 to 2% by weight of citric acid; Adding purified water to the silkworm powder mixed composition obtained in the above step, kneading and cutting; Wherein the cuts obtained in the above step are fried in a hot water at 170 to 180 DEG C for 90 to 100 seconds.
제 1항의 방법에 따라 제조된 항산화 및 혈당강하 활성을 갖는 식감과 기호도가 향상된 것이 특징인 누에 과자.The silkworm cake according to claim 1, wherein the silkworm cake having antioxidant activity and hypoglycemic activity is improved.
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JP2005229981A (en) * 2004-02-20 2005-09-02 Mineshita Takeshi Processed food using silkworm powder under optimal condition as food
JP2008506393A (en) 2004-07-19 2008-03-06 ザ プロクター アンド ギャンブル カンパニー Low carb snack food
KR100821466B1 (en) * 2007-02-07 2008-04-11 주식회사오뚜기 How to make retort tuna balls or tuna steak

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JP2005229981A (en) * 2004-02-20 2005-09-02 Mineshita Takeshi Processed food using silkworm powder under optimal condition as food
JP2008506393A (en) 2004-07-19 2008-03-06 ザ プロクター アンド ギャンブル カンパニー Low carb snack food
KR100821466B1 (en) * 2007-02-07 2008-04-11 주식회사오뚜기 How to make retort tuna balls or tuna steak

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200079215A (en) 2018-12-24 2020-07-02 주식회사 마린테크노 Composition for Silkworm-collagen and manufacturing method thereof

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