KR101893219B1 - Body fluid analyzing method and body fluid analyzing system using the same - Google Patents

Abstract

A body fluid analysis method and a body fluid analysis system using the same are disclosed. The body fluid analysis system according to the present invention comprises at least one sample containing portion including a light emitting portion for generating and irradiating light of a specific wavelength, a reflector for reflecting light irradiated from the light emitting portion, and a groove for receiving a body fluid sample, A sample analyzer for analyzing the body fluid sample by measuring a concentration of the body fluid sample based on the electrical signal converted by the light receiving unit and a light receiving unit irradiating the light emitting unit and collecting light reflected from the sample receiving unit and converting the light into an electrical signal, . Accordingly, the body fluid component can be more accurately analyzed even with a small amount of sample, thereby diagnosing the user's health condition and providing the diagnosis result to the user.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a body fluid analysis method and a body fluid analysis system using the same.

The present invention relates to a body fluid analysis method and a body fluid analysis system using the body fluid analyzing method, and more particularly, to a body fluid analyzing method and a body fluid analysis system using the body fluid analyzing method, And a body fluid analysis system using the same.

Generally, a patient's health condition is diagnosed by analyzing a body fluid of a patient and measuring the amount or concentration of a substance (indicator substance) related to the health condition of the patient such as a disease present in the body fluid. At this time, the measurement of the indicator substance is carried out through immunoassay or biochemical reaction such as antigen antibody reaction to the indicator substance by collecting body fluid of the patient.

In the past, the use of reagents or equipment that required expert knowledge in these tests was costly and time consuming, as well as being able to receive a diagnosis only after a visit to the hospital.

In addition, the conventional inspection method requires a lot of manual work, and errors in the result caused errors in the work.

Recently, a point-of-care test (POCT) has emerged that overcomes the disadvantages of this method. On-site inspection refers to a test to diagnose a patient in a short period of time by immediately collecting and analyzing body fluids in a place where the patient is present. On-site testing is widely used because of its various advantages such as simple diagnosis of the patient and additional cost and time saving.

These field inspections include immunoassays such as Tnl, BNP, CK-MB, cholesterol (TG, TC, HDL), glucose, liver disease measurement (AST, ALT) and creatinin, and biochemical analysis. Such a biosensor has been used various methods including an electrochemical optical measurement method.

However, there is still a problem that the on-site inspection that provides the convenience of body fluid analysis should be able to obtain more precise values and perform the analysis and show the result to the user.

SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a body fluid analysis method and a body fluid analysis system using the same that can diagnose a health state of a user by analyzing body fluid components more accurately. It is another object of the present invention to provide a body fluid analysis method capable of analyzing a body fluid using a small amount of samples and a body fluid analysis system using the same.

According to an aspect of the present invention, there is provided a body fluid analysis system including: a light emitting unit for generating and irradiating light of a specific wavelength; At least one sample accommodating portion including a reflector for reflecting light irradiated from the light emitting portion and a groove for receiving a body fluid sample; A light receiving unit for collecting light reflected from the sample accommodating unit irradiated from the light emitting unit and converting the collected light into an electrical signal; And a sample analyzer for analyzing the body fluid sample by measuring the concentration of the indicator substance of the body fluid sample based on the electrical signal converted in the light receiving section.

The sample accommodating portion may have a transparent plate-like structure.

And a substrate for fixing the sample accommodating portion, wherein the sample accommodating portion can be removably attached to the substrate.

The light emitting unit may include a light emitting diode (LED) that emits light having a predetermined wavelength.

The light receiving unit is composed of a photodiode, and can convert the intensity of the reflected light into a voltage.

The sample analyzer may also perform a body fluid analysis by calculating the molar concentration of the body fluid sample using a Lambert-Beer's law or a Kubelka-Munk equation.

The reflective material may be a white or silver membrane to reflect the light emitted from the light emitting unit.

The sample receiving portion may also include a dye causing a color reaction with the received bodily fluid sample.

And the sample accommodating portion is connected to the groove and includes a sample injecting portion for moving the body fluid sample; And a dye injecting unit connected to the groove or the sample injecting unit to transfer a dye causing a color reaction with the received bodily fluid sample.

The light generated by the light emitting unit may have a wavelength ranging from 400 nm to 650 nm.

And a display unit for displaying a result analyzed by the sample analyzing unit to a user.

According to another aspect of the present invention, there is provided a body fluid analysis method comprising: generating light of a specific wavelength and irradiating a body fluid sample; Collecting light reflected from the body fluid sample; Converting the collected light into an electrical signal; And calculating the concentration of the analyte in the body fluid sample based on the converted electrical signal to perform body fluid analysis.

And performing the body fluid analysis can be performed by calculating the molar concentration of the body fluid sample using the Lambert-Beer's law or the Kubelka-Munk equation .

In addition, the collecting of the light may include collecting the light reflected by the reflecting member provided in the area collected in the body fluid sample.

And displaying the analysis result performed in the step of performing the body fluid analysis to the user.

And color-reacting the body fluid sample and the dye before the step of irradiating the light.

And performing the body fluid analysis may include analyzing the body fluid by calculating the molar concentration of the body fluid sample using light reflected from the body fluid sample that caused the color reaction with the dye.

According to the bodily fluid analysis method and the bodily fluid analysis system using the bodily fluid analysis method according to the present invention, the permeation measurement method used in the conventional solution is applied as a reflection type so that the bodily fluid component can be more accurately analyzed even with a small amount of sample, And provide the diagnosis result to the user.

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a view for roughly explaining the principle of obtaining a molar concentration of a body fluid sample,
FIG. 2 is a view showing a configuration of a body fluid analysis system according to an embodiment of the present invention,
FIGS. 3A and 3B are diagrams illustrating an operation process of a body fluid analysis system according to an embodiment of the present invention;
FIGS. 4A and 4B are graphs showing effects of using a reflective material in a body fluid analysis system according to an embodiment of the present invention,
5 is a view showing a configuration of a body fluid analysis system according to another embodiment of the present invention,
6 is a view for explaining a color reaction of a body fluid sample and a dye,
FIGS. 7A and 7B are views showing a configuration of a body fluid analysis system according to another embodiment of the present invention,
8 is a flow chart illustrating a method for analyzing body fluids according to an embodiment of the present invention.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Terms including ordinals, such as first, second, etc., may be used to describe various elements, but the elements are not limited to these terms. The terms are used only for the purpose of distinguishing one component from another.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, . The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

In the following description of the present invention with reference to the accompanying drawings, the same components are denoted by the same reference numerals regardless of the reference numerals, and redundant explanations thereof will be omitted. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a view for roughly explaining the principle of obtaining a molar concentration of a body fluid sample. FIG. The molar concentration of the body fluid sample can be determined by the lambert-beer's law. It is also possible to use the Kubelka-Munk equation in addition to the Lambert-Beer law. Here, we briefly introduce the Lambert-Beer law. The Lambert-Beer law is defined by the following equation (1).

Where I _o is the intensity of the incident light and I _t is the intensity of the light transmitted through the solution. Ε is the molar extinction coefficient, l is the light transmission distance, and C is the molar concentration. This law log (I _o / I _t) is the thickness of the material to absorb light, that is, that the Lambert proportional to the distance (l) of the light transmitted through the laws and log (I _o / I _t) absorb light (Beer's law) which is proportional to the concentration of the substance (C)

1, the light emitted from the light emitting unit 10 passes through the cuvette 20 containing the solution 5, and is collected by the light receiving unit 30. As shown in FIG.

Here, the light emitting unit 10 generates light of a desired wavelength. When the generated light passes through the cuvette 20, light is partially absorbed in the solution contained in the cuvette 20, and light not absorbed enters the light receiving unit 30 through the cuvette 20.

The intensity of the light generated and collected by the light emitting unit 10 and the light receiving unit 30 and the distance between the cuvette 20 can be known by experiment and the molar absorptivity is also constant for each compound. Therefore, according to the Lambert- The molar concentration (C) can be obtained.

However, in such cases, a large amount of body fluid sample is needed because light travel distance is required. That is, since the body fluid necessary to fill the volume of the cuvette 20 to some extent is required, it is inconvenient to extract a small amount of blood from the human body.

In order to solve such problems, the present invention provides a body fluid analysis method capable of analyzing body fluid even with a small amount of body fluid using another structure, and a body fluid analysis system using the same.

2 is a view showing a configuration of a body fluid analysis system according to an embodiment of the present invention. As shown in FIG. 2, the body fluid analysis system according to an embodiment of the present invention includes a light emitting unit 100, a sample receiving unit 110, and a light receiving unit 130. A reflector 120 is provided under the sample storage part 110.

The light emitting unit 100 has a function of generating light of a specific wavelength and irradiating the sample to the sample storage unit 110 to be described later. Specifically, the light emitting unit 100 is composed of a light emitting unit that generates light of a specific wavelength. That is, a light emitting unit such as a light emitting diode (LED) may be applied. In particular, the light emitting unit 100 can generate light of a specific wavelength such as 430 nm, 470 nm, 565 nm, and 635 nm.

The sample accommodating portion 110 has a function of accommodating a body fluid sample or the like. That is, a groove having a predetermined depth is provided so that the body fluid sample can be received. The sample storage unit 110 may be composed of one unit or two or more units.

The reflector 120 has a function of reflecting light irradiated from the light emitting portion 100. Specifically, the reflector 120 may be formed of a white or silver membrane to facilitate reflection of light. The material may be of any material that is already in use, so long as it does not absorb light and can reflect. The reflector 120 is provided at a lower portion of the sample accommodating portion 110 and is irradiated by the light emitting portion 100 to reflect light passing through the body fluid sample.

The light receiving unit 130 has a function of collecting the light reflected by the reflector 120 after passing through the body fluid sample irradiated from the light emitting unit 100 and in the sample accommodating unit 110. More specifically, the light receiving unit 130 may be formed of a photodiode. The light receiving unit 130 collects light reflected from the sample storage unit 110 irradiated from the light emitting unit 100 using a photodiode, and converts the collected light into an electrical signal.

2, the electrical signal generated by the light receiving unit 130 is sent to a sample analyzer (not shown) for measuring the concentration of a sample of body fluid, and the sample analyzer measures the concentration of the sample of body fluid, Analyze how much of the ingredient is contained. At this time, the above-mentioned Lambert-Beer law is used, and conventional methods can be used in this regard.

The molten extinction coefficient of the compound solution and the intensity of the light emitted from the light emitting unit 100 can be determined in advance by using the depth, the incident angle, and the reflection angle of the groove of the sample accommodating unit 110, Since the intensity of the reflected light can be measured by the light receiving unit 130, the molar concentration of the indicator material can be obtained by the Lambert-Beer law. An indicator substance is a substance to be analyzed for concentration of components contained in blood.

Here, the body fluid sample in the sample accommodating portion 110 reacts with the dye to cause color change depending on the concentration, and the body fluid sample causing the color change reduces the intensity of the incident light and reflects it.

FIGS. 3A and 3B illustrate an operation of a body fluid analysis system according to an embodiment of the present invention.

As shown in FIG. 3A, a substrate 140 for inserting the sample accommodating portion 110 may be present. A reflector 120 may be attached to a lower portion of the sample storage unit 110 at a portion where the sample storage unit 110 is fixed in the structure of the substrate 140.

3B, when the sample holder 110 is mounted on the substrate 140, a white or silver color membrane is attached to the lower portion of the sample holder 110, so that the light incident from the light emitting unit 100 is effectively reflected And is collected in the light receiving unit 130.

Here, the substrate 140 and the sample accommodating portion 110 may be made of a transparent acrylic material, but are not limited to any particular material.

On the other hand, the entire substrate 140 may be made of a white or silver material so as to facilitate reflection. In this case, the reflective material 120 may be omitted. That is, the substrate 140 itself can replace the function of the reflector.

4A and 4B are graphs showing the effect of using a reflective material in a body fluid analysis system according to an embodiment of the present invention.

FIG. 4A shows the reflectance according to the color change of a sample of a body fluid which caused a color reaction with a dye when a reflector composed of a white membrane is not used. As shown in FIG. 4A, when there is no reflective material, the degree of reflection is irregular according to the color change. Thus, without a reflector, the molar concentration of the body fluid sample can not be determined by the color change as in the body fluid analysis system according to an embodiment of the present invention. Here, the abscissa represents the degree of color change, that is, the change of the concentration due to the dye, and the ordinate represents the ratio of the reflected light to the incident light, that is, the reflectance (%).

On the other hand, FIG. 4B shows the reflectance according to the color change of the body fluid sample causing the color reaction with the dye in the case of using the reflector composed of the white membrane. As shown in FIG. 4B, the reflectance changes uniformly according to the color change, that is, the concentration change of the body fluid sample causing the color reaction with the dye. Specifically, as the concentration increases, the reflectance tends to decrease.

4A, it is difficult to use the Lambert-Beer law because of the irregular change of the reflectance depending on the degree of color reaction. However, when a reflector composed of a white membrane is used as shown in FIG. 4B Since the change of the reflectance according to the degree of the color reaction is constant, the molar concentration of the body fluid sample can be obtained by using the Lambert-Beer law.

5 is a view showing a configuration of a body fluid analysis system according to another embodiment of the present invention. Specifically, FIG. 5 shows the configuration of the sample accommodating portion 110 in the body fluid analysis system.

As shown in FIG. 5, the sample accommodating portion 110 includes a groove 116 for collecting body fluid samples. And a sample injection unit 112 connected to the groove 116 to move the body fluid sample. In Figure 5 the dye injector 114 is located between the sample injector 112 and the groove 116 so that the body fluid sample And injected into the sample injecting section 112 to flow to the groove 116 and react with the dye to be collected in the groove 116.

The sample accommodating portion 110 may be made of a transparent material. Although not shown in FIG. 5, a reflector 120 is disposed under the sample accommodating unit 110, and the light emitting unit 100 irradiates a sample of the body fluid stored in the recess 116 with light, The light reflected by the light receiving unit 130 is collected and converted into an electric signal to perform a fluid sample analysis.

On the other hand, FIG. 6 is a diagram for explaining the color reaction of the body fluid sample and the dye. The configuration of the sample accommodating portion 110 is assumed to be the configuration shown in FIG. 5, but may be the configuration of the sample accommodating portion 110 shown in FIG. 3A.

As shown in FIG. 6, when a body fluid sample reacts with a dye, a color change is caused according to the concentration of the reactant present in the body fluid sample. For example, if the concentration of cholesterol present in the body fluid is zero, it will not cause any color change. However, as the concentration of cholesterol increases, the color of the compound causing the color reaction becomes darker. This color reaction can be performed using different dyes depending on the body fluid component to be measured, and the color is changed to blue, red, green, etc., and the concentration of the color varies depending on the concentration of the body fluid component as described above. Dyes such as ADOS, ADPS, ALPS, DAOS, HDAOS, MADB, MAOS, TOB, TOOS, TOPS and the like can be used as the result of the chemical reaction between the body fluid component and the dye. Since this is only a dye that has already been used, explanations relating to the chemical reaction formula of the body fluid component and the dye and the specific components of the dye will be omitted because they may obscure the essence of the invention.

7A and 7B are diagrams showing a configuration of a body fluid analysis system according to another embodiment of the present invention.

7A is different from FIG. 5 in that a dye injection unit 114 is separately provided. That is, the sample of the body fluid injected from the sample injection unit 112 causes a color reaction with the dye injected from the dye injection unit 114 to be accommodated in the groove 116. The lower portion of the groove 116 includes a reflector (not shown) that reflects the irradiated light as described above. Accordingly, the light intensity can be obtained by the light emitting portion and the light receiving portion, and the molar concentration of the body fluid sample can be measured.

FIG. 7B shows a sample accommodating portion having a plurality of grooves 116 unlike FIG. 7A. Below the plurality of grooves 116, a reflector 120 is included as shown in FIG. 7B to reflect light. On the other hand, in FIG. 7B, the sample injection unit 112 is shown as one, but it goes without saying that the number of the sample injection units 112 may be one or more. 7B may be separately provided with the dye injection unit 114 as shown in FIG. 7A, but the plurality of grooves 116 may be provided with separate dyes.

8 is a flow chart illustrating a method for analyzing body fluids according to an embodiment of the present invention.

First, light of a specific wavelength is generated (S200). The subject that generates light of a specific wavelength may be an LED. The generated light is irradiated to the body fluid sample to collect the reflected light (S210, S220). As described above, a reflector is provided to reflect the light irradiated to the body fluid sample. Also, the subject that collects the reflected light is the light-receiving unit described above.

Thereafter, the light receiving unit converts the collected light into an electric signal, and calculates the concentration of the body fluid sample using the Lambert-Beer law (S230, S240). The sample analysis unit may perform this (S250) and this may be provided in a separate configuration.

8, the bodily fluid is limited to blood, but the bodily fluids other than blood may be analyzed by the same method as the flow of Fig.

In addition, it is to be understood that the present invention is not limited to the above-described embodiments, and various changes and modifications may be made without departing from the scope of the present invention as defined by the appended claims. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

100:
110 ...................................... Sample accommodating portion
120 ...................................... Reflector
130 .............................. Receiver

Claims (17)

A light emitting unit for generating and irradiating light of a specific wavelength;
At least one sample receiving portion including a plurality of grooves for receiving a reflector for reflecting light irradiated from the light emitting portion and a body fluid sample;
A light receiving unit for collecting light reflected from the sample accommodating unit irradiated from the light emitting unit and converting the collected light into an electrical signal; And
And a sample analyzer for analyzing the body fluid sample by measuring an indicator substance concentration of the body fluid sample based on the electrical signal converted by the light receiving unit,
The reflector may include a white or silver membrane to reflect the light emitted from the light emitting unit,
Wherein the sample receiving portion comprises a dye causing a color reaction with the received bodily fluid sample,
The sample-
A sample injection unit connected to the plurality of grooves for moving the body fluid sample; And
And a dye injecting unit connected to the plurality of grooves or the sample injecting unit to move a dye causing a color reaction with the received bodily fluid sample,
Wherein the reflector is disposed under the plurality of grooves. The method according to claim 1,
Wherein the sample accommodating portion has a plate-like structure of a transparent material. The method according to claim 1,
And a substrate for fixing the sample accommodating portion,
Wherein the sample accommodating portion is removably attachable to the substrate. The method according to claim 1,
Wherein the light emitting unit includes a light emitting diode (LED) that emits light having a predetermined wavelength. The method according to claim 1,
Wherein the light receiving unit is composed of a photodiode and converts the intensity of the reflected light into a voltage. The method according to claim 1,
The sample analyzing unit analyzes,
Characterized in that the body fluid analysis is performed by calculating the molar concentration of the body fluid sample using the Lambert-Beer's law. delete delete delete The method according to claim 1,
Wherein the light generated by the light emitting unit has a wavelength ranging from 400 nm to 650 nm. delete delete delete delete delete delete delete

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