KR101828972B1 - Manufacturing Method for Liquid Type Product Comprising Seed Oil of Korean Raspberry - Google Patents

Abstract

The present invention relates to a method for producing a soft capsule containing brambly seed oil. More specifically, the method comprises the steps of extracting brambled seed oil, purifying the brambled seed oil, and emulsifying the brambled seed oil into a liquid product.
The brambled seed oil of the present invention contains omega-3, omega-6 fatty acid and conjugated linoleic acid, which are unsaturated fatty acids, and can be provided as a functional food for improving blood circulation.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a method for producing a liquid product comprising brambled seed oil,

The present invention relates to a method for producing a liquid product containing brambly seed oil. More specifically, the method comprises the steps of extracting brambled seed oil, purifying the brambled seed oil, and emulsifying the brambled seed oil into a liquid product.

The brambled seed oil of the present invention contains omega-3, omega-6 fatty acids and conjugated linoleic acid, which are Unsaturated Fatty Acids, and can be provided as a functional food for improving blood circulation.

Black raspberry is the fruit of the bokbunja strawberry tree, a perennial deciduous shrub belonging to the rose family. In Korea, Jeollabuk - do province is known as a major producing region. Bokbunja harvests sour red berries in the early summer and is used for edible or herbal medicine such as tonic, kangjeongje, liver protection, physical weakness, immobilization, oil well and urination. It is known that epicatechin, catechine, and procyanidin B-4 are present in the stem of the bokbunja, and kaempferol, quercetin, quercetin 3-O-β-D-glucuronoide, ellagic acid, and sanguin H-5 exist in the leaves. do. Quercetin and phenolic compounds are also present in the bokbunja fruit. In addition, immature and Edo phenolic compounds exist as gallotannin, elaarge tannin, gallic acid, and 2,3- (S) -HHDP-D-glucopyranoside. On the other hand, it was found that the ingredients extracted from bramble seeds contained active ingredients such as omega-3, omega-6, ellagitannins, elagic acid, palmitic acid, stearic acid and oleic acid including conjugated linoleic acid. In addition, bokbunja seed oil is a complex of materials related to blood circulation improvement. Omega-3, linolenic acid, a precursor of gamma linolenic acid, and a large amount of antioxidants are most suitable. Therefore, tocopherol, which can prevent the oxidation of unsaturated fatty acid, which is the biggest problem of conventional omega-3 blood circulation improving food, is contained, and various active ingredients such as conjugated linolenic acid as well as various polyphenols It is expected that the effect of blood circulation improvement will be enhanced more than the existing omega-3 products. Furthermore, the inventors of the present invention have found out that a variety of functional materials such as omega-3 and omega-6 fatty acids and conjugated linoleic acid are known as a result of a study on the extraction of the bokbunja seed oil and the study of the ingredients (patent application) Thereby completing the present invention.

Thrombosis and embolism due to hematological disorders are the causes of serious cardiovascular diseases such as myocardial infarction and stroke. In addition, there is a risk that blood circulation is caused by erroneous eating habits and lifestyle, hyperlipidemia, hypertension and diabetes. .

Platelet and hematopoietic cells (red blood cells and platelets) are mainly involved in blood circulation improvement. They maintain homeostasis of blood flow, maintain normal hemostasis and protection function in injured or inflamed areas of blood vessels, excessive activation of coagulation factors in plasma, And hemorrhagic diseases such as atherosclerosis and stroke are generated. Thus, excessive hemostatic action and formation of blood clogs interfere with the flow of blood, resulting in hemodilution abnormality, and blood clotting thrombus). Therefore, improvement of blood circulation through ingestion of health functional foods is very important for the prevention of chronic adult diseases before such treatment (hemodialysis) occurs.

Blood circulation maintains blood flow homeostasis of plasma and blood cells constituting blood, maintains normal function of human body by maintaining normal hemostasis and protection function in injured area or inflammation part of blood vessel. However, excessive activation of coagulation factors in the plasma, promotion of platelet aggregation, and erythrocyte deformity break down the homeostasis of blood flow, resulting in cardiovascular diseases such as arteriosclerosis and stroke. The endothelial cells of the blood vessels are substances that break down blood clots and are an important part of regulating blood flow and velocity. When this endothelial cell is damaged by some stimulus, cholesterol builds up in it and forms a plaque. The portion of the blood vessel where the plaque is piled up swells, and the blood vessel narrows, which may interfere with the flow of blood. The blood flow is closely related to blood cholesterol, fat and glucose, so reducing intake of animal fat and salt, and increasing the intake of vegetables, fruits, fish, and grains can help the flow of blood.

In order to improve blood circulation, it is necessary to lower the cholesterol level in the blood or relax the blood vessels. HMG (3-hydroxy-3-methylglutaryl) -CoA reductase is an enzyme which plays an important role in cholesterol biosynthesis. Are used as inhibitors of HMG-CoA reductase. Therefore, it is known that the activity of HMG-CoA reductase is regulated to regulate blood cholesterol level to improve blood circulation. In addition, nitrogen monoxide (NO) is a very important messenger molecule involved in nervous system, immune system, cardiovascular system, etc. in relaxing blood vessels. NO stimulates endothelium-dependent vasorelaxation by stimulating guanylate cyclase (GC) in vascular smooth muscle cells to increase 3 ', 5'-cyclic guanosine monophosphate (cGMP). sodium nitroprusside acts as a vasodilator by stimulating guanylate cyclase of smooth muscle to increase the production of cGMP. The prior art related to the present invention is Korean Patent Registration No. 10-0650016 (a method for separating an antioxidant active substance from a bokbunja) To obtain a residue, and after extraction, an antioxidative active substance is separated using an aromatic adsorbent. Korean Patent Laid-Open No. 10-2012-0119265 (antioxidant functional composition and functional food containing the same) contains omega, ginseng, daphnia and bokbunja as an active ingredient, and addition of raw persimmon juice and honey to form a high-antioxidant functional composition And foods. However, these prior arts have different technical ideas and technical configurations from the present invention using brambly seed oil.

It is an object of the present invention to provide a health functional food for improving blood circulation using an unsaturated fatty acid contained in bramble-derived seed oil. Conventional bokbunja were made by making bokbunja or bokbunja pulp processed product using functional ingredients contained in pulp, but bokbunja was discarded. The present invention is to provide a health functional food for improving blood circulation or a composition thereof by using an unsaturated fatty acid and an active ingredient contained in brambly seed oil. Conventional general medicines for blood circulation improvement have various effects besides improvement of blood circulation because health functional foods contain a complex component in comparison with Saracen (Donga Pharmaceutical) and aspirin (Bayer). Vitamin E enhances endocrine function and has antioxidant properties. Ginkgo biloba is a preventive treatment for diphtheria, and safflower oil helps to improve obesity. Omega 3 can help pregnant women who want to have arthritis anti-inflammatory activity and normal development of the fetal vision system. have.

The present invention is to provide a health functional food for improving blood circulation or a composition thereof by using an unsaturated fatty acid and an active ingredient contained in brambly seed oil.

The present invention relates to a method for producing a liquid product comprising extracting brambled seed oil, purifying the brambled seed oil, and emulsifying the brambled seed oil into a liquid product.

The brambled seed oil of the present invention contains omega-3, omega-6 fatty acid and conjugated linoleic acid, which are unsaturated fatty acids, and can be provided as a functional food for improving blood circulation.

1 is a manufacturing process diagram of the present invention.

The present invention relates to a method for producing a liquid product comprising extracting brambled seed oil, purifying the brambled seed oil, and emulsifying the brambled seed oil into a liquid product.

Oil of seed oil is obtained by separation, drying, roasting, milking, centrifugation and filtration. Omega-3 was 23.8 ~ 33.8% and Omega-6 was 42.6 ~ 49.9% by analysis of fatty acid composition, content and other components of brambled seed oil (Korea Food Research Institute, Suwon Women's University) The content was confirmed. Other contents of tocopherol were found to be 8.3 mg / 100 g, conjugated linoleic acid was 1.11 mg / g, total polyphenol was 0.68 mg / g and total flavonoid was 1.13 mg / g.

The purification of the seed oil is carried out by a de-oxidation, deoxidation and decolorization process. In the above, emulsification of seed oil is composed of a step of primary oiling with polyglycerol fatty acid ester, a step of secondary oiling with lecithin, a step of tertiary oiling with brambly seed oil, and a step of quaternizing oil with guanidine gum. Homogenizing the brambled seed oil emulsified in the fourth order, mixing the additive (7 kinds), homogenizing again, and sterilizing.

1) Seed Oil

After the brambled seeds are separated and dried, seed oil is roasted at 135 to 145 캜 for 3 to 5 minutes, milked at 125 to 135 캜 for 1 to 10 minutes, centrifuged and filtered.

2) Purification of seed oil

Defection; 97% by weight of oil and 3% by weight of water are mixed and stirred at 80 DEG C and centrifuged at 4,000 rpm for 10 minutes to observe the bacterium seed oil.

Deoxidation; The pink solution prepared by adding NaOH and phenol phthalene corresponding to 2 wt% of the total weight of the NaOH to the decolorized bramblor seed oil was centrifuged at 4,000 rpm for 10 minutes at 70 ° C in hot water, , White, transparent liquid) to deoxidize bramble seed oil.

decolorization; Activated carbon corresponding to 5% by weight of the total weight of the deacidized brambled seed oil is added to the deoxidized brambled seed oil, stirred at 100 ° C and centrifuged to obtain a supernatant, which is filtered and discolored to obtain brambled seed oil.

3) In the above,

(4%) of polyglycerol fatty acid ester at 4,000 rpm for 20 minutes at 70 DEG C, and a step of secondary lyophilizing 0.4 g of lecithin at 4,000 rpm for 20 minutes at 70 DEG C and 2 mL of brambled seed oil (20%) at 4,000 rpm to 70 ° C for 20 minutes, and a step of quaternizing with 0.04 g of guar gum at 4,000 rpm at 70 ° C for 20 minutes.

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≪ Example 1-4 >

10kg of bramble seeds were collected, washed, separated, dried, roasted, milked, centrifuged and filtered to obtain bramble seed oil. The seed oil was defected, deoxidized and decolorized and purified to obtain 1 kg of seed oil.

7 to 7.3 ml of distilled water and 0.3 to 0.5 g of polyglycerol fatty acid ester are firstly emulsified at 4,000 rpm at 70 DEG C and then 0.3 to 0.5 g of lecithin is further mixed and emulsified at 4,000 rpm to 70 DEG C. 1 to 3 ml of brambled seed oil was mixed with the above emulsifier, and the emulsion was tertiary emulsified at 4,000 r to 70 ° C for 20 minutes. Then, 0.03 to 0.05 ml of guar gum was added and emulsified for 4 minutes at 4,000 r for 20 minutes.

10 ml of brambled seed oil emulsified over the fourth order was homogenized at 700 ° C at 700 bar, and the amount of purple grape 0.8-1.2 ml, fructooligosaccharide 4.5-5.5 ml, citric acid 0.04-0.06 ml, citron 0.2-0.4 ml , 0.2 to 0.4 ml of blueberry flavor, 2.5 to 3.5 ml of chickberry puree, and 0.003 to 0.005 ml of grapefruit seed extract. Homogenization at 700 DEG C again at 70 DEG C and sterilization at 90 DEG C for 15 minutes yielded a homogeneous (emulsified) solution. The brambled seed oil emulsion was made into a liquid product and put into an ampule maker to make an ampoule.

≪ Test Example 1 > Acid value measurement of purified brambled seed oil

Mix approximately 20 μl of phenolphthalein solution (indicator) with 5 ~ 10g of neutral ethanol and Erlenmeyer flask with 1: 2 mixture of ether (1: 2) stopper. Titrate with 0.1 N ethanolic aqueous solution of potassium hydroxide (pale red for 30 seconds .

. Acid value =

S: Amount of sample (g)

a: consumption of 0.1 N ethanolic potassium hydroxide solution (mL)

b: Consumed amount (mL) of 0.1 N ethanolic potassium hydroxide solution to a blank test (ethanol, ether mixture (1: 2) 100 mL)

f: Potency of 0.1N ethanolic potassium hydroxide solution

When the specimen is colored, the indicator is tested by using 1% thymolphthalein, an alcohol solution or a 2% alkali blue-6B alcohol solution or by increasing the amount of the test substance by a small amount. 2% alkali blue-6B is used. The acid value of bokbunja oil was measured to be 1.0187 mg KOH / g oil.

≪ Test Example 2 > Peroxide measurement of purified brambled seed oil

Take 1 to 5 g of the sample and 25 mL of chloroform (3: 2) as the acetic acid, add 1 mL of saturated potassium iodide solution (sample and acetic acid, slightly warmed as necessary) while gently shaking the chloroform, Shake and shake, and add 1 mL of starch solution (indicator). Titrate with 0.01 N sodium thiosulfate solution.

Peroxide value =

a: Titration number of 0.01 N sodium thiosulfate solution (mL)

b: Consumed amount of 0.01 N sodium thiosulfate solution in the blank test (mL)

f: Potency of 0.01 N sodium thiosulfate solution

The peroxide value of bramble seed oil was 1.0107 meq / kg oil.

<Application Example 1>

According to the method of allowing the composition of the present invention as a health functional food method, 20 ml of brambled seed oil was injected into an injection tube made of plastic material using an ampoule, and the ampoule was made by sealing the upper part.

&Lt; Application example 2 >

A 10 ml ampoule was prepared in the same manner as in Application Example 1.

The brambled seed oil of the present invention contains omega-3, omega-6 fatty acids and conjugated linoleic acid, which are unsaturated fatty acids, and thus can be used as a functional food for improving blood circulation, and thus is industrially applicable.

Claims (9)

delete delete delete Extracting brambled seed oil; Refining the extracted brambled seed oil; Emulsifying the purified brambly seed oil into a liquid product by mixing with an additive,
The refined brambled seed oil was prepared by mixing 97 wt% of oil and 3 wt% of water and stirring at 80 캜 and then centrifuging at 4,000 rpm for 10 minutes to observe the brambled seed oil. The defatted bramblor seed oil was mixed with NaOH, And 2% by weight of phenolphthalene. The resulting pink solution was washed with hot water at 70 DEG C and then centrifuged at 4000 rpm for 10 minutes to deoxidize the brambled seed oil. Adding activated carbon corresponding to 5% by weight of the total weight of the deoxidized bramblor seed oil, stirring the mixture at 100 DEG C and centrifuging to obtain a supernatant, filtering and decolorizing the supernatant,
Wherein the emulsification of the brambled seed oil comprises a step of primary oiling with polyglycerin fatty acid ester, a step of secondary lyophilizing with lecithin, a step of trihydrating with brambly seed oil, and a step of quaternizing with gum gum. For producing a liquid product containing The method according to claim 4, wherein the brambled seed oil is obtained by separating, drying, roasting, milking, centrifuging and filtering the brambled seeds to produce a liquid product containing the brambled seed oil delete delete The method of claim 4, wherein the liquid product of brambly seed oil comprises a step of homogenizing the emulsified brambled seed oil, a step of mixing the base material, a step of secondarily homogenizing the liquid obtained by mixing the brambler seed oil and the additive, A method for producing a liquid product containing a bramble seed oil, comprising the steps of: delete

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