KR101744966B1 - Functional cosmetic composition - Google Patents

Abstract

The present invention relates to a functional cosmetic composition. In one embodiment, the functional cosmetic composition is selected from the group consisting of Psidium guajava ) extract, garlic ( Allium sativum extract, Luffa aegyptiaca extract and black tea extract as active ingredients.

Description

Functional cosmetic composition {FUNCTIONAL COSMETIC COMPOSITION}

The present invention relates to a functional cosmetic composition. More particularly, the present invention relates to a functional cosmetic composition comprising a complex extract having excellent SNARE complex formation inhibitory effect.

Muscle contraction and relaxation is caused by the release of neurotransmitters called acetylcholine from neurons. In order for these neurotransmitters to be released, there must be a protein that is a receptor complex called SNARE (soluble Nethylmaleimide-sensitive factor attachment protein receptor; SNAP receptor), which helps the membrane and membrane to fuse.

More specifically, in neurons, a membrane can be formed by membrane fusion between a synaptic vesicle containing a neurotransmitter and a presynaptic membrane, and a channel for releasing a neurotransmitter may be opened to relax and relax the muscle . At this time, the fundamental force of membrane fusion is composed of three protein complexes: Syntaxin protein, SNAP-25 (Synaptosomal-associated protein 25) protein, and VAMP-2 (vesicle-associated membrane protein 2) protein.

Biomembranes are strongly pressed against each other due to their characteristics. These membranes are not fused spontaneously. They are given a strong force from the outside to overcome the repulsive force between the membranes. At this time, the SNARE protein produces strong force enough to overcome the repulsive force between the membranes. These SNARE proteins, which are twisted like tweaked, are a key phenomenon of exocytosis involving the release of neurotransmitters.

If membrane fusion by the SNARE complex is inhibited, it is possible to suppress the generation of wrinkles caused by the movement of muscles by the inhibitory effect of SNARE formation and to improve the generated wrinkles, and to treat hyperhidrosis caused by excessive excretion of neurotransmitters Can be improved.

Guavava ( Psidium guajava ) is a plant belonging to the plumaceae family of tropical America, and is cultivated in tropical regions, Taiwan, and Okinawa. Guava fruit is juicy, sweet and contains a lot of vitamins. It is usually used as a raw material for eating, canning, japanese jam and jam. Guava leaves include polyphenolic materials, tannins, saponins, ellagic acid glycosides and flavonoids.

Meanwhile, garlic ( Allium sativum ) belongs to the lily family and is a perennial plant with scaly stalk and contains about 0.1% of essential oil. The main components of the essential oil are diallyl disulfide, trisulfodialyl, and allyl disulfide.

Luffa aegyptiaca is a perennial vine plant with bark and its components include ash, carotene, saponin, gibblin, and galactic. Also, it contains a large amount of polyphenols such as flavonoids and quercetin.

Black tea is produced by oxidizing the leaves of tea tree ( Camellia sinensis ). It is known that when oxidized by the enzymes of the tea leaf, it becomes reddish and polyphenols such as teaplavin and tearubigen are produced.

BACKGROUND ART [0002] The background art relating to the present invention is disclosed in Korean Patent Publication No. 2000-0061029 (published on Oct. 16, 2000, entitled " Hyperhidrosis Treatment Agent).

It is an object of the present invention to provide a functional cosmetic composition excellent in wrinkle-reducing effect, sweat gland control, and suppression effect.

Another object of the present invention is to provide a functional cosmetic composition which is free from cytotoxicity, is harmless to human body and has little skin irritation.

It is still another object of the present invention to provide a functional cosmetic composition which is excellent in formulation stability and skin-tearing property.

Another object of the present invention is to provide a functional cosmetic composition having excellent productivity and economy.

One aspect of the present invention relates to a functional cosmetic composition. In one embodiment, the cosmetic composition is a Psidium guajava ) extract, garlic ( Allium sativum extract, Luffa aegyptiaca extract and black tea extract as active ingredients.

In one embodiment, the cosmetic composition may have the effect of suppressing the formation of SNARE complex and controlling the release of neurotransmitters to improve skin wrinkles and controlling and inhibiting sweat secretion.

In one embodiment, the guava extract, the garlic extract, the scrubs extract and the black tea extract may be contained in a weight ratio of 1: 0.1~3: 0.05~3: 0.05~3.

In one embodiment, the guava extract, the garlic extract, the scrubber extract and the black tea extract are obtained by preparing a solvent extract of guava, garlic, loofah and black tea using an extraction solvent, respectively, May contain at least one of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane and alcohols having 1 to 10 carbon atoms.

In one embodiment, the guava extract, the garlic extract, the scrubs extract and the black tea extract may be contained in an amount of 0.001 to 50 wt% based on the total weight of the cosmetic composition.

In one embodiment, the cosmetic composition may be a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, foundation, wax or spray formulation.

The functional cosmetic composition of the present invention is free from cytotoxicity, is harmless to the human body, has less irritation to the skin, is excellent in controlling and suppressing skin wrinkles and sweat glands through inhibition of formation of SNARE complex and regulation of neurotransmitter release, And excellent sweat gauge control effect. Therefore, it is excellent in spreadability when applying the skin, and the manufacturing cost is low, so that productivity and economy can be excellent.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to be exemplary, self-explanatory, allowing for equivalent explanations of the present invention.

Functional Cosmetics  Composition

One aspect of the present invention relates to a functional cosmetic composition. In one embodiment, the cosmetic composition is a Psidium guajava ) extract, garlic ( Allium sativum extract, Luffa aegyptiaca extract and black tea extract as active ingredients.

In the present specification, the above-mentioned extract is a solvent extract obtained by extracting guava, garlic, water cucumber and black tea with an extraction solvent, a diluted solution or concentrate of the solvent extract, a dried product obtained by drying the solvent extract, Or purified water, and may be the result of fractionation by a solvent fractionation method, and may include both a polar solvent fraction and a non-polar solvent fraction.

In one embodiment, the Psidium guajava ) extracts can be obtained using one or more of the guava leaves, fruits, stems and seeds.

The garlic ( Allium sativum extract can be obtained using at least one site from the garlic side (garlic side), leaves and flowers of garlic.

The Luffa aegyptiaca extract can be obtained using at least one of the stems, leaves, flowers and fruits of a cucumber.

The black tea extract can be obtained by using leaf parts of black tea produced by oxidizing leaves of Camellia sinensis .

In one embodiment, the extract may be obtained by pulverizing or sieving the above-described guava, garlic, loofah and black tea to a size of 0.01 mm to 10 cm. Here, the " size " is defined to mean " maximum length ".

In one embodiment, the guava extract, the garlic extract, the scrubs extract and the black tea extract can be extracted using solvent extraction. In one embodiment, the guava extract, the garlic extract, the scrubs extract and the black tea extract may include a step of preparing a solvent extract using the guava, garlic, loofah, and black tea extractants.

In one embodiment, the extraction solvent may include at least one of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane, and alcohols having 1 to 10 carbon atoms.

In one embodiment, the alcohols having 1 to 10 carbon atoms include lower monohydric alcohols such as methanol, ethanol, butanol, pentanol, and hexanol; alcohols such as 1,2-pentanediol, 1,5-pentanediol, hexanediol, , Intermediate dihydric alcohols such as octanediol and decanediol, lower trivalent alcohols such as propylene glycol, 1,3-butylene glycol and glycerin, and the like can be used. These may be used alone or in combination of two or more. For example, the solvent may be a mixture of ethanol and water. For example, a solvent containing 70% by weight of ethanol and 30% by weight of water may be used.

In one embodiment, the solvent extraction is carried out by adding 100 parts by weight of guava, garlic, loofah and black tea to 100 to 3000 parts by weight of solvent, and extracting the mixture at a temperature of 10 to 90 DEG C for 1 to 10 days . When the extraction temperature and the extraction time are applied, the extraction efficiency is excellent and denaturation of the active ingredient can be prevented.

In one embodiment, the guava extract, the garlic extract, the scrubs extract and the black tea extract are immersed in the extraction solvent and then subjected to one or more extraction methods such as stirring extraction, reflux cooling, cold extraction, ultrasonic extraction and supercritical extraction The filtrate can be further purified by filtration. Further, the filtrate can be obtained by repeating the above extraction and filtration process a plurality of times. When the solvent is extracted under the above conditions, the functional cosmetic composition is cytotoxic and harmless to the human body, and can be excellent in skin wrinkle improvement and sweat secretion controlling effect.

In another embodiment of the present invention, the above-mentioned guava extract, garlic extract, sponge cucumber extract, and black tea extract are prepared by concentrating each of the obtained solvent extracts to prepare a concentrate; And fractionating the concentrate with a fraction solvent to prepare a fraction extract.

The concentrate can be concentrated by evaporation or concentration under reduced pressure. More specifically, the filtrate can be concentrated by charging it into a rotary vacuum evaporator (Eyela, Japan).

In one embodiment, the fraction extract may be prepared by introducing the concentrate into a funnel and fractionating by adding a separating solvent.

The fractionating solvent may include at least one of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane and alcohols having 1 to 10 carbon atoms.

In another embodiment, the fraction extract is obtained by fractionating the concentrate using a first separating solvent to prepare a first fraction extract; And extracting the first fraction with a second fraction solvent to prepare a second fraction extract. At the time of fraction extraction under the above conditions, guava extract, garlic extract, roasted cucumber extract and black tea extract containing phenolic compounds and polysaccharides in a high content can be obtained.

The first fraction solvent and the second fraction solvent may each contain at least one of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane and alcohols having 1 to 10 carbon atoms. In one embodiment, the first fraction solvent comprises water, and the second fraction solvent comprises an alcohol having from 1 to 10 carbon atoms. The alcohol having 1 to 10 carbon atoms may include butanol (n-BuOH).

In one embodiment, the guava extract, the garlic extract, the scrubber extract and the black tea extract may be mixed with the solvent extract obtained by solvent extraction of guava, garlic, loofah, and black tea, respectively.

In another embodiment, the guava extract, the garlic extract, the scrubber extract, and the black tea extract may be obtained by extracting a mixture containing the guava, the garlic, the sponge cucumber and the black tea by solvent extraction.

In one embodiment, the cosmetic composition may contain the guava extract, the garlic extract, the water-repellent extract and the black tea extract in a weight ratio of 1: 0.1~3: 0.05~3: 0.05~3. When included in the above weight ratio range, an unpredictable synergism between the active ingredients contained in the extract is generated, and the SNARE complex formation inhibitory effect and neurotransmitter release inhibiting effect are excellent, and the formulation stability of the cosmetic composition is excellent have. For example, the guava extract, the garlic extract, the scrubber extract and the black tea extract may be contained in a weight ratio of 1: 0.1-1: 0.05-1: 0.05-1. As another example, it may be contained in a weight ratio of 1: 0.1 to 0.5: 0.1 to 0.3: 0.1 to 0.3.

In one embodiment, the guava extract may be contained at a higher content than the garlic extract, the scrubs extract, and the black tea extract.

In one embodiment, the garlic extract may be contained at a higher content than the scrubby extract and the black tea extract.

In one embodiment, the water droplet extract may be included in an amount of the black tea extract or more.

In one embodiment, the guava extract, the garlic extract, the scrubs extract and the black tea extract may be contained in an amount of 0.001 to 50 wt% based on the total weight of the cosmetic composition. Within this range, it is cytotoxic and harmless to human body, and is excellent in skin wrinkle improvement and sweat secretion controlling effect, and can be excellent in formulation stability of the functional cosmetic composition.

The guava extract, the garlic extract, the scrubber extract and the black tea extract may inhibit the formation of the SNARE complex and inhibit the release of neurotransmitters. The Guava extract, Garlic extract, Sponge cucumber extract, and Black tea extract have similar effects to Botox used in the treatment of wrinkles of skin, and natural materials can be used to improve them without side effects. The cosmetic composition of the present invention has a low production cost and a high productivity as compared with other products having similar effects, so that the cosmetic composition of the present invention is excellent in economical efficiency have.

In one embodiment, the cosmetic composition may further comprise a base component that may be incorporated into a conventional cosmetic composition. The specific examples may further include a solubilizer, a surfactant, a moisturizer, an agitator, a pH adjuster, a preservative, an antioxidant, a sequestering agent, a bactericide, an anti-inflammatory agent, an antimicrobial agent, a solubilizing agent or a flavoring agent, .

Surfactants that can be included in the cosmetic composition according to the present invention include anionic surfactants such as ammonium laurylsulfosuccinate and ammonium lauryl sulfate and nonionic surfactants such as polyoxyethylene alkyl ether and polyoxyethylene fatty acid ester This is possible. Also, cationic surfactants such as tertiary aliphatic amine salts, alkyltrimethylammonium chloride and the like and amphoteric surfactants such as betaine, amide betaine and the like can be used.

The moisturizing agent may be sodium hyaluronate, glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, etc. The thickener may be xanthan gum, methyl cellulose, hydroxyethyl cellulose, carrageenan, carboxy Methylcellulose, hydroxymethylcellulose, and other water-soluble polymeric compounds. The pH adjuster may specifically include citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid, and the like.

The preservative may be benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDM, and the like. Specific examples of the antioxidant include dibutylhydroxytoluene and ascorbic acid .

The metal ion sequestering agent may be disodium ethylenediaminetetraacetate, tetranodium ethylenediaminetetraacetate, and the like. Specifically, the disinfectant may be chlorhexidine gluconate, quaternary ammonium salt, pyrrothonol amine, zinc pyrithione suspension, urethropropylbutyl Carbamate, salicylic acid, and the like.

Examples of the anti-inflammatory component include monoammonium glycyrrhizinate, dipotassium glycyrrhizinate, allantoin, and mixtures thereof. The antibacterial (antimicrobial) component specifically includes phenoxyethanol, chlorhexidine, chlorhexidine gluconate, benzoic acid and its salts, benzyl Alcohols, salicylic acid, trichlorocarbane, zinc pyrithione suspensions, and mixtures thereof.

The solubilizing agent may specifically be a phage 60 -hydrogenated castor oil or the like, and the above-mentioned flavoring agent may be any conventional agents used in the cosmetic composition field of the present invention.

In one embodiment, the cosmetic composition may have a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, foundation, wax or spray formulation.

Hereinafter, the structure and operation of the present invention will be described in more detail with reference to preferred embodiments of the present invention. It is to be understood, however, that the same is by way of illustration and example only and is not to be construed in a limiting sense.

The contents not described here are sufficiently technically inferior to those skilled in the art, and a description thereof will be omitted.

Example  And Comparative Example

(a) Guava extract: Psidium guajava ) 100 parts by weight were washed with purified water, dried, and then sieved to a size of 0.1 to 3 mm using a blender. Subsequently, a solvent containing 700 parts by weight of ethanol and 300 parts by weight of water was added, stirred for 3 days at room temperature, filtered through whatman No. 2 filter paper, and extracted twice by the same method as above Followed by filtration to prepare a solvent extract. Then, the solvent extract was concentrated under reduced pressure in a rotary concentrator to prepare a concentrate, followed by lyophilization to obtain a guava extract.

(b) Garlic extract: garlic ( Allium 100 parts by weight of the scaly stem (garlic side) of saliva and sativum were washed with purified water, dried and then cut into a size of 0.1 to 3 mm using a blender. Subsequently, a solvent containing 700 parts by weight of ethanol and 300 parts by weight of water was added, stirred for 3 days at room temperature, filtered through whatman No. 2 filter paper, and extracted twice by the same method as above Followed by filtration to prepare a solvent extract. Then, the solvent extract was concentrated under reduced pressure in a rotary concentrator to prepare a concentrate, followed by lyophilization to obtain a garlic extract.

(c) Scarlet Cucumber Extract: 100 parts by weight of leaves, fruit and stem of Luffa aegyptiaca were washed with purified water, dried and then cut into a size of 0.1 to 3 mm using a blender. Subsequently, a solvent containing 700 parts by weight of ethanol and 300 parts by weight of water was added, stirred for 3 days at room temperature, filtered through whatman No. 2 filter paper, and extracted twice by the same method as above Followed by filtration to prepare a solvent extract. Then, the solvent extract was concentrated under reduced pressure in a rotary concentrator to prepare a concentrate, which was then lyophilized to obtain a water-salted cucumber extract.

(d) Black Tea Extract: 100 parts by weight of black tea leaves were washed with purified water, dried and then cut into 0.1 to 3 mm size using a blender. Subsequently, a solvent containing 700 parts by weight of ethanol and 300 parts by weight of water was added, stirred for 3 days at room temperature, filtered through whatman No. 2 filter paper, and extracted twice by the same method as above Followed by filtration to prepare a solvent extract. Then, the solvent extract was concentrated under reduced pressure in a rotary concentrator to prepare a concentrate, which was then lyophilized to obtain a black tea extract.

Example  One

The combined extracts containing the guava extract, the garlic extract, the water-repellent extract and the black tea extract at a weight ratio of 1: 0.5: 0.2: 0.2 were prepared.

Example  2

The combined extracts containing the guava extract, the garlic extract, the scrubs extract and the black tea extract in a weight ratio of 1: 0.8: 0.3: 0.3 were prepared.

Example  3

The combined extracts containing the guava extract, the garlic extract, the water-repellent extract and the black tea extract in a weight ratio of 1: 2: 1: 1 were prepared.

Example  4

The combined extracts containing the guava extract, the garlic extract, the scrubby extract and the black tea extract at a weight ratio of 1: 1: 2: 2 were prepared.

Comparative Example  One

The combined extracts were prepared containing the garlic extract, the scrubby extract and the black tea extract in a weight ratio of 1: 0.4: 0.4.

Comparative Example  2

The combined extracts containing the guava extract, the water-sweat extract and the black tea extract at a weight ratio of 1: 5: 0.2 were prepared.

Comparative Example  3

The combined extracts containing the guava extract, the garlic extract, and the scrubber cucumber extract at a weight ratio of 1: 6: 3 were prepared.

Comparative Example  4

The combined extract containing the guava extract, the garlic extract and the black tea extract at a weight ratio of 1: 0.5: 0.2 was prepared.

Test Example  1: Cytotoxic effect test of compound extract

The WST assay measures the mitochondrial dehydrogenase activity in cells by measuring live cells. WST is an aqueous tetrazolium salt that reacts with living cells to produce an orange-soluble formazan. The absorbance of formazan is measured to confirm cytotoxicity.

To the above Examples 1 to 4 and Comparative Examples 1 to 4, the toxicity to PC12, a cell derived from chromosome-positive cell type, was measured. The PC12 was dispensed into a 96-well plate and cultured for 24 hours. Then, the concentrations of 0, 12.5, 25, 50, 100, 200, 400, and 800 (占 퐂 / ml) were measured for Examples 1 to 4 and Comparative Examples 1 to 4, , And the cells were placed in each well and cultured for 24 hours. Thereafter, the supernatant was removed, and the WST solution was mixed, reacted for 2 hours, and absorbance was measured at 450 nm. The degree of cytotoxicity was calculated according to the following formula 1, and the results are shown in Table 1 below.

[Formula 1]

Cytotoxic activity (Cell Viability) = (absorbance of sample treated group / absorbance of sample treated group (control group)) X 100

division Cytotoxic activity (%) Control group (100 占 퐂 / ml) 100 Example 1 (100 占 퐂 / ml) 99.2 Example 2 (100 占 퐂 / ml) 98.2 Example 3 (100 占 퐂 / ml) 97.5 Example 4 (100 占 퐂 / ml) 97.1 Comparative Example 1 (100 占 퐂 / ml) 97.5 Comparative Example 2 (100 占 퐂 / ml) 96.2 Comparative Example 3 (100 占 퐂 / ml) 97.1 Comparative Example 4 (100 占 퐂 / ml) 98.2

Referring to Table 1, it can be seen that Examples 1 to 4 of the present invention did not show cytotoxicity at a survival rate of 90% or more in PC12, a cell derived from chromosome-positive cell type.

Experimental Example  2: by combination extract Membrane fusion  Inhibitory effect of phenomenon

In order to confirm the inhibition of the membrane fusion phenomenon, the membrane fusion inhibitory effect of ethanol as the control group and the extracts of Examples 1 to 4 and Comparative Examples 1 to 4 was confirmed. Phospholipid-1, 2-dioleoyl-sn-glycero-3-phosphatidylcholine (POPC, 62 mol%), 1,2-diol to produce liposome, -Glycero-3-phosphatidylserine (DOPS, 35 mol%), 1,2-diololeyl-sn-glycero-3-phosphocerine-N- (7-nitro-2-1,3-benzoxadiazole V-vesicle was prepared by mixing Rhodamin-PE (1.5 mol%), a fluorescent substance (NBD-PE, 1.5 mol% Then, liposomes (t-vesicles) were prepared by mixing DOPS and POPC to prepare liposomes without fluorescent substance labeling. To form a complex of purified SNAP25 and Syntaxin 1a, a fluorescently unmarked liposome was bound, and VAMP2 was conjugated with a liposome containing fluorescent material. Thereafter, the two liposomes were mixed and the fluorescence intensity was measured using a fluorescence intensity meter. Thereafter, the two liposomes were mixed and the fluorescence intensity was measured at a fluorescence wavelength of 465 nm / 530 nm (excitation / emission) and 465 nm / 625 nm (excitation / emission) Respectively.

The fluorescence wavelength of NBD-PE was set to 465nm / 530nm (excitation / emission), and the fluorescence wavelength of Rhodamine was set to 465nm / 625nm (excitation / emission) so Rhodamine could absorb the emission wavelength of NBD-PE. The above method is called a fluorescence resonance energy transfer (FRET) system. As the membrane fusion between T-vesicle and V-vesicle progresses, the distance between the BD-PE and Rhodamine becomes farther, , The absorbance value of NBD-PE increases and the value of Rhodamine decreases.

For each of Examples 1 to 4 and Comparative Examples 1 to 4, the intensity was obtained using the absorbance measured over time, and then the initial measurement value was set to 0% of membrane fusion and the final measurement value The membrane fusion rate was calculated by converting the membrane fusion to 100%, and the membrane fusion ratio was determined. The membrane fusion ratio was determined by the FRET method to calculate the maximum membrane fusion inhibition value. The results are shown in Table 2 below. At this time, the concentrations of Examples 1 to 4 and Comparative Examples 1 to 4 used in the measurement experiment were 50 占 퐂 / ml.

division Membrane conversion (%) Control group 100 Example 1 (50 占 퐂 / ml) 75.1 Example 2 (50 占 퐂 / ml) 77.5 Example 3 (50 占 퐂 / ml) 84.2 Example 4 (50 占 퐂 / ml) 83.0 Comparative Example 1 (50 占 퐂 / ml) 88.4 Comparative Example 2 (50 占 퐂 / ml) 91.2 Comparative Example 3 (50 占 퐂 / ml) 90.5 Comparative Example 4 (50 占 퐂 / ml) 89.4

The results are shown in Table 2. Referring to Table 2, the membrane fusion rates of Examples 1 to 4 were lower than those of Comparative Examples 1 to 4, and the extracts of Examples 1 to 4 containing guava extract, garlic extract, , It was found that the SNARE complex had a membrane fusion inhibitory effect as compared with Comparative Examples 1 to 4. This shows that it exerts excellent skin wrinkle or sweat gland control effect.

Experimental Example  3: Inhibition of Neurotransmitter Release by PC12 Cells by Combined Extract

For the complex extracts of Examples 1 to 4 and Comparative Examples 1 to 4, in order to measure the effect of inhibiting neurotransmitter release in PC12 cells, the PC12 cells were cultured until they were grown to about 50% to 60% Respectively. The cells were treated with Nerve Growth Factor (NGF) to differentiate into neurons. Krebs' buffer with high K + (56 mM NaCl, 1.2 mM MgSO ₄ , 2.5 mM CaCl ₂ , 68 mM KCl, 24 mM NaHCO ₃ , 2 mM KH ₂ PO ₄ , 11 mM Dextrose, pH 7.4), and cultured in a 5% CO ₂ incubator at 37 ° C for 15 minutes. After washing with Krebs' buffer (118 mM NaCl, 1.2 mM MgSO ₄ , 2.5 mM CaCl ₂ , 5 mM KCl, 24 mM NaHCO ₃ , 2 mM KH ₂ PO ₄ , 11 mM Dextrose, pH 7.4) Comparative Examples 1 to 4 50 占 퐂 / ml were mixed and the medium was replaced. After incubation in a 5% CO ₂ incubator at 37 ° C for 15 minutes, the supernatant was recovered. The supernatant containing the secreted neurotransmitter was quantitated using noradrenalin ELISA kit (CEA907Ge, USCN) and the release rate of norepinephrine was calculated. The results are shown in Table 3 below.

division Norepinephrine concentration (%) Control group 100 Example 1 (50 占 퐂 / ml) 60.6 Example 2 (50 占 퐂 / ml) 66.5 Example 3 (50 占 퐂 / ml) 71.5 Example 4 (50 占 퐂 / ml) 73.2 Comparative Example 1 (50 占 퐂 / ml) 80.5 Comparative Example 2 (50 占 퐂 / ml) 81.2 Comparative Example 3 (50 占 퐂 / ml) 79.3 Comparative Example 4 (50 占 퐂 / ml) 77.5

Referring to Table 3, it was found that the extracts of Examples 1 to 4 were superior in the release of norepinephrine, a neurotransmitter, compared to Comparative Examples 1 to 4. Thus, It was confirmed that there was an inhibitory effect on the release of neurotransmitters, and it was found that the sweat secretion and control effect were excellent.

Experimental Example  4: Formulation stability evaluation

The cosmetic compositions of the cream formulations were prepared by applying the components and contents of the following Tables 4 and 5 to the combined extracts of Examples 1 to 4 and Comparative Examples 1 to 4 (Production Examples 1 to 8). Then, the creams of Preparation Examples 1 to 8 were stored for one week at 50, 40, 25, and 5 day light conditions, respectively, and the degree of separation and discoloration was compared and the results are shown in Table 6 below. The degree of separation and discoloration of the product was classified into the following six grades and evaluated.

* Separation and discoloration evaluation criteria

4: Severely separated (discolored), 5: Severely separated (discolored), 2: slightly separated (discolored), 3: slightly separated (discolored)

Category (Unit:% by weight) Manufacturing example One 2 3 4 Cetearyl alcohol 1.5 1.5 1.5 1.5 Self emulsifying glycerin monostearate One One One One Pro-type glycerin monostearate 2 2 2 2 Monta wax 0.8 0.8 0.8 0.8 Shea Butter One One One One Triglycerine 5 5 5 5 Dimethicone 5 5 5 5 Cyclopentacyoxane 4 4 4 4 vegetable oil 2.5 2.5 2.5 2.5 Pentaerythrityl tetraisostearate One One One One Disodium iodide 0.05 0.05 0.05 0.05 Polyethylene glycol One One One One Betaine 1.5 1.5 1.5 1.5 1,3-butylene glycol 4 4 4 4 Concentrated glycerin 6 6 6 6 Carbomer 0.12 0.12 0.12 0.12 antiseptic 0.7 0.7 0.7 0.7 extract Example 1
10 Example 2
10 Example 3
10 Example 4
10 Purified water 52.83 52.83 52.83 52.83 Sum 100 100 100 100

Category (Unit:% by weight) Manufacturing example 5 6 7 8 Cetearyl alcohol 1.5 1.5 1.5 1.5 Self emulsifying glycerin monostearate One One One One Pro-type glycerin monostearate 2 2 2 2 Monta wax 0.8 0.8 0.8 0.8 Shea Butter One One One One Triglycerine 5 5 5 5 Dimethicone 5 5 5 5 Cyclopentacyoxane 4 4 4 4 vegetable oil 2.5 2.5 2.5 2.5 Pentaerythrityl tetraisostearate One One One One Disodium iodide 0.05 0.05 0.05 0.05 Polyethylene glycol One One One One Betaine 1.5 1.5 1.5 1.5 1,3-butylene glycol 4 4 4 4 Concentrated glycerin 6 6 6 6 Carbomer 0.12 0.12 0.12 0.12 antiseptic 0.7 0.7 0.7 0.7 extract Comparative Example 1
10 Comparative Example 2
10 Comparative Example 3
10 Comparative Example 4
10 Purified water 52.83 52.83 52.83 52.83 Sum 100 100 100 100

Evaluation items Degree of separation and discoloration Manufacturing example One 2 3 4 5 6 7 8 50 ℃ One One 2 One 3 2 2 3 40 ℃ 0 One One One 2 2 2 2 25 ℃ 0 0 0 0 One 0 One 0 5 ℃ 0 0 0 0 0 0 0 0 daylight 0 0 One One 3 3 2 3

The results are shown in Table 6. Referring to Table 6, Examples 1 to 4 of the present invention were superior to Comparative Examples 1 to 4 in terms of formulation stability under high temperature and daylight conditions. In Comparative Examples 1 to 4, 4, the formulation stability was lowered.

Experimental Example  5: Sensory evaluation

In order to evaluate the effect of wrinkle improvement and feeling of use of the present invention, a questionnaire survey was conducted on a product group composed of 30 adult women aged 20 to 40 years. The cream form cosmetic compositions of Preparation Examples 1 to 8 prepared in Experimental Example 4 were used in the morning at the last stage of skin care, and then a questionnaire item (wetting, moisturizing, persisting and wrinkle-improving effect) And the results are shown in Table 7 below. ≪ tb > < TABLE >

Survey item Manufacturing example One 2 3 4 5 6 7 8 Brittle 4.4 4.2 4.0 3.9 3.6 3.4 3.1 3.3 Moistness 4.5 4.3 3.9 3.8 3.6 3.5 3.2 3.5 Persistence 4.4 4.2 3.9 4.0 3.7 2.9 3.1 3.2 Wrinkle improvement effect 4.3 4.2 4.0 4.1 3.4 3.3 3.6 3.0

With reference to the results of Table 7, it was confirmed that Production Examples 1 to 4 (cosmetic compositions of Examples 1 to 4 of the present invention) were excellent in spreading and tactile sensation, excellent in control of sweat secretion amount and inhibitory effect, It was found that the durability was improved even in the case of Production Examples 5 to 8 (Comparative Examples 1 to 4), and the wrinkle-improving effect was lowered and the spreadability and touch were lowered than those of Production Examples 1 to 4.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (6)

The present invention includes an extract of Psidium guajava , Allium sativum extract, Luffa aegyptiaca extract and black tea extract as active ingredients at a weight ratio of 1: 0.1~3: 0.05~3: 0.05~3,
Wherein the composition has an effect of suppressing the formation of a snare (SNARE) complex formation and controlling the release of a neurotransmitter to improve wrinkles of the skin and controlling the secretion of sweat.
delete delete The method according to claim 1, wherein the guava extract, the garlic extract, the scrubs extract,
Guava, garlic, loofah cucumber, and black tea, respectively, using an extraction solvent,
Wherein the extraction solvent comprises at least one of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane and alcohols having 1 to 10 carbon atoms.
[Claim 2] The cosmetic composition according to claim 1, wherein the guava extract, garlic extract, sorghum extract and black tea extract are contained in an amount of 0.001 to 50 wt% based on the total weight of the cosmetic composition.
The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, foundation, wax or spray.