KR101650020B1 - Cosmetic composition for improving skin wrinkle containing snowberry extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing Snowberry Extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition containing a Snowberry Extract, Thereby improving the skin wrinkles. The cosmetic composition of the present invention is superior to conventional skin wrinkle-reducing agents in stability in formulation and exhibits an effect similar to that of Botox, which has been used in conventional wrinkle-improving procedures, thereby greatly improving the wrinkles of the skin without side effects.

Snowberry, Foje, SNARE complex, Wrinkle improvement

Description

Technical Field [0001] The present invention relates to a cosmetic composition for improving skin wrinkles containing snowberry extract,

The present invention relates to a cosmetic composition containing Snowberry Extract as an active ingredient. More particularly, the present invention relates to a cosmetic composition containing a Snowberry Extract, Thereby improving the skin wrinkles. The cosmetic composition of the present invention is superior to conventional skin wrinkle-reducing agents in stability in formulation and exhibits an effect similar to that of Botox, which has been used in conventional wrinkle-improving procedures, thereby greatly improving the wrinkles of the skin without side effects.

Synaptic vesicles containing neurotransmitters at the time of neurotransmitter release must be fused with the presynaptic membrane to form a channel between the two interfaces. At this time, the protein that provides the fundamental force of membrane fusion is three protein complex called SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor). Membrane fusion between synaptic vesicles and synaptic membrane opens the neurotransmitter exit pathway, which involves the t-SNARE complex attached to the target membrane and v-SNARE attached to the vehicle. t-SNARE is a complex of two proteins called synaptaxin 1a and SNAP-25. These SNARE proteins are twisted like twine. Upon such membrane fusion, rearrangement of the lipid bilayer, which is well known in the art, occurs. Since the biomembranes are pushing strongly against each other, these membranes are not fused spontaneously, but strong forces are exerted from the outside to overcome the repulsive force between the membranes. At this time, the SNARE protein produces a strong force enough to overcome the repulsive force between the membranes. In other words, the formation of the SNARE complex is a source of power overcoming the interlaminar repulsive force and a key phenomenon of exocytosis including the release of neurotransmitters. On the other hand, there is a neuromuscular junction in the upper layer of the muscle to control muscle relaxation and contraction, and the nerve terminal is loaded with synaptic vesicles. BoNT (Botulinum neurotoxin, hereinafter referred to as Botox) is a protease that cleaves the SNARE protein, a key protein involved in neurotransmitter release. Botox cleaves the SNARE protein and blocks neurotransmission, resulting in paralysis of the muscle cells that have infiltrated Botox. While Botox blocks the transmission of nerves by irreversibly cleaving the SNARE protein, the so-called "Botox Bottle" is a sort of "competitive inhibitor" that interferes with neurotransmission by inhibiting the formation of the SNARE complex. Argyrenelin, a major component of the currently available Botox product, has a weakness that its effect is uncertain, the price of synthetic peptides is disadvantageous, and it is not consumer friendly.

Development of inhibition of neurotransmission by using natural extracts can provide much less expensive raw materials than peptides and it is a natural material so it can be approached to consumers in the end product development easily. do.

On the other hand, the swallowing method is referred to as the traditional medicine technique of the oriental medicine, such as the harmony, the medicine, the numerology, the spore, the law, and the examination. This is a pharmaceutical technology that changes the original properties of medicinal materials by processing the medicinal materials based on the oriental medicine theory.

Foamer is a term that includes selection of the correct form of the drug, elimination of fecal matter, and purification, heat treatment, and processing using ancillary substances. In the case of Foje, it is possible to apply the medicine together with the application, which is in line with the needs of the medical care and to achieve the purpose of the medicine according to the dialectic. Because of the variety of types of drugs used in the formulations of drugs, they have different properties and actions, and the actions caused by these drugs are different. There are relatively many kinds of currently used pills, and they can be classified into liquid pills and solid pills. Liquid ingredients include alcohol, vinegar, salt water, honey, ginger juice, licorice juice, black bean juice, saline, unmeasured, mayo oil, milk, Solid feeds include rice, wheat bran, alfalfa, tofu, soil, rice flour, or sand.

Purpose of herbal medicines is to clean drugs, facilitate storage, reduce or eliminate the toxicity and side effects of medicines, change medicines to make medicines more effective, enhance the therapeutic effect of medicines, To remove the bad smell and taste of the good.

Skin wrinkles are an apparent feature of the most prominent skin aging caused by an endogenous factor such as age and stress, or by external environmental factors such as air pollution or ultraviolet radiation. The wrinkles occur on the face, neck, neck, elbow, armpits, hands and feet of the body such as the forehead, the eyes, the eyes and the mouth. Most of them begin to become noticeable around 30 years old, and their number, depth and range increase with age. The major cause of wrinkles is the formation of crosslinks in collagen fibers and elastic fibers, which ultimately occupy the majority of skin dermis components, and changes in the production and degradation of these fibers. can do. Antioxidants such as vitamin E, beta-carotene, vitamin C and glutathione, radical scavengers and substances promoting dermal collagen synthesis such as vitamin A, which are used to control skin aging, Has been used as a material. However, most of these cosmetic active ingredients are strongly irritated by the skin or are unstable in the formulations themselves, resulting in discoloration, detachment and sedimentation, and the inherent activity of the raw material is reduced.

Recently, various cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. These natural materials are not only less harmful to the skin, but also become more appreciated as a raw material for cosmetics as consumers' response increases.

Accordingly, the inventors of the present invention have studied about natural raw materials having an excellent skin wrinkle-reducing effect, and found that the skin wrinkle-improving effect is excellent when a cosmetic composition is prepared using Snowberry extract, and completed the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition for improving skin wrinkles containing Snowberry extract as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for improving skin wrinkles containing Snowberry extract as an active ingredient.

The cosmetic composition according to the present invention is excellent in the effect of inhibiting the formation of the SNARE complex and preventing the release of neurotransmitters to improve skin wrinkles by containing Snowberry extract as an active ingredient, Less skin irritation can be reduced. In addition, it has excellent stability in the formulation as compared with conventional skin wrinkle reducing agents, and exhibits an effect similar to that of Botox, which has been used in conventional wrinkle remover procedures, thereby significantly improving the wrinkles of the skin without side effects.

The present invention provides a cosmetic composition for improving skin wrinkles containing Snowberry extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

Snowberry used in the present invention is a plant of Symphoricarpos which is a deciduous shrub of the plant and is about 15 kinds of small species. All but one are native to North America and Central America, and one is native to West China. It is also called waxberry. The fruit with a diameter of 1-2 cm is smooth and has a color from white (common snowberry) to pink (little leaf snowberry), red (coralberry), and purple (Chinese coralberry) Each color represents different colors. The representative types of snowberries are as follows.

Common snowberry ( Symphoricarpos albus )

· Mexican snowberry ( Symphoricarpos mexicanus )

· Little leaf snowberry ( Symphoricarpos microphyllus )

· Creeping snowberry (Creeping snowberry, Symphoricarpos mollis )

· Western Snowberry or Wolfberry (Western snowberry or Wolfberry, Symphoricarpos occidentalis )

· Coralberry ( Symphoricarpos orbiculatus )

· Mountain snowberry (Mountain snowberry, Symphoricarpos oreophilus )

· Round leaf snowberry (Roundleaf snowberry, Symphoricarpos rotundifolius )

· Chinese coralberry ( Symphoricarpos sinensis )

The present invention discloses a composition containing Snowberry extract.

Snowberry is a common snowberry ( Symphoricarpos albus ), Mexican Snowberry ( Symphoricarpos mexicanus ), Little leaf snowberry ( Symphoricarpos microphyllus ), Creeping snowberry ( Symphoricarpos mollis ), Western snowberry ( Symphoricarpos occidentalis ), Coralberry ( Symphoricarpos orbiculatus , Mountain Snowberry ( Symphoricarpos oreophilus ), Round leaf Snowberry ( Symphoricarpos rotundifolius ), Chinese Coral Berry ( Symphoricarpos sinensis ) and all kinds of snowberries.

The snowberry of the present invention is excellent in the effect of inhibiting the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex and inhibiting the release of neurotransmitters, thereby improving skin wrinkles. This shows a similar effect to Botox that has been used in the prior art for the treatment of skin wrinkles, thereby greatly improving the wrinkles of the skin without side effects.

The snowberry extract according to the present invention can be subjected to spray treatment before extracting the snowberry with a solvent. Snow berry is treated by boiling, steaming, roasting, roasting, or mixing of dry snow berry. More specifically, in case of boiling, it is preferable to boil at 60 to 100 ° C for 30 minutes to 2 hours at 120 to 150 ° C for 3 to 6 hours at steaming, at 100 to 180 ° C for 10 minutes to 1 hour at 80 to 100 ° C for roasting, It is easily selected from 10 minutes to 1 hour. If the treatment temperature and the treatment time are exceeded, the effect of the fogging treatment may be deteriorated or the composition may be changed.

The present invention can also be used as a feedstock for use in the present invention. Examples of the feedstuffs that can be used in the present invention include sake, vinegar, salt water, honey, ginger juice, licorice juice, black bean juice, saline, Rice, wheat bran, alfalfa, tofu, soil, mulberry or sand.

The extraction process of the snowberry of the present invention can be carried out by a conventional method known in the art. For example, water or an organic solvent is added to fog-treated snowberry, and the mixture is subjected to reflux extraction, followed by filtration, filtration and centrifugation to separate the residue and filtrate, and the separated filtrate is concentrated under reduced pressure to obtain Snowberry extract have.

The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, preferably 80% ethanol. At this time, the extraction temperature is preferably 10 to 80 ° C, and the extraction time is preferably 6 to 24 hours. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed.

After obtaining the extract using a solvent, the extract can be obtained by cooling, heating and filtering at room temperature by a conventional method known in the art, or the solvent can be further evaporated, spray dried or freeze-dried.

The cosmetic composition according to the present invention contains Snowberry extract in an amount of 0.0001 to 30% by weight based on the total weight of the composition. If the content is less than 0.0001% by weight, the effect of improving wrinkles of the skin can not be obtained. If the content is more than 30% by weight, the increase of the effect is not so large.

The cosmetic composition according to the present invention may be formulated into a flexible lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, a pack, a foundation, a lipstick or a powder foundation.

In the cosmetic composition of each formulation, the ingredients other than the above-mentioned extract may be mixed and selected without difficulty by the person skilled in the art according to the formulation or use purpose of other cosmetic ingredients.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples. However, these examples and test examples are only intended to facilitate understanding of the present invention, and the scope of the present invention is not limited to these examples.

[Example 1] Production of Snowberry bovine (raw) extract

To 1 kg of dried snowberry, 5 L of an 80% ethanol aqueous solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 285 g of Snowberry extract.

[Example 2] Preparation of snowberry black tea extract

1 kg of dried snowberries were placed in a suppository container, roasted at 150 ° C for 30 minutes, and then dried in the shade. After adding 5 L of an aqueous 80% ethanol solution, the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 250 g of Snowberry fresh mint extract.

[Example 3] Preparation of snowberry juice extract

1kg of dried snowberry is immersed in 300ml of sulfur (1 ~ 30% of the dry weight of snowberry in a cool shade using the traditional inge) at a rate of 20 ~ 30% Gt; C < / RTI > for 4 hours and then dried in the shade. After adding 5 L of 80% aqueous ethanol solution, the mixture was refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 260 g of Snowberry extract.

[Example 4] Preparation of Snowberry seed salt extract

1 kg of dried snowberry is mixed with 200 ml of brine (aged using a conventional onion in a cool shade, concentration of salt water is 8 to 10% and used in a ratio of 20 to 30% with respect to the dry weight of snowberry) The mixture was allowed to stand for 1 hour to be absorbed, and the Snowberry, in which the salt water was completely absorbed, was put into a container of 150 ° C., roasted for 30 minutes, and then dried in the shade. After adding 5 L of 80% aqueous ethanol solution, the mixture was refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 295 g of Snowberry seed extract.

[Test Example 1] Effect of membrane fusion inhibition

The membrane fusion inhibitory effect on the Snowberry extract obtained in Examples 1 to 4 was tested. SNARE proteins called SNAP25 (NMO11428), Trust ShinA1 (AF217197) and VAMP2 (NMO12663) were purified from E. coli Codon (+) RIL, Novagen transformed by biotechnology to produce SNARE proteins . First, a mixture of palmitoyloleoyl phosphatidylcholine (POPC) (62 mol%), Dioleoyl phosphatidylserine (DOPS) (35 mol%) to make a liposome which is a microscopic coat particle with fluorescent substance labeling, 1-oleoyl-2- [N- (7-nitro-2,1,3-benzoxadiazol-4-yl) amino] caproylphosphatidylserine (1.5mol%) and Rhodamin-PE (1.5mol%), which is a fluorescent substance, were mixed to prepare a 10mM lysozyme (v -vesicle), and to make a liposome without a fluorescent substance, DOPS and POPC were mixed at a molar concentration of 35:65 to form a 50-mM liposome (t-vesicle). The mixture was mixed at a molar concentration of 1: 1 and reacted at room temperature for 1 hour to prepare a complex composed of purified SNAP25 and authentic Shin-1a. The mixture was mixed so that the molar ratio of the liposome with the fluorescent substance was 100: 1, VAMP2 was conjugated to a liposome containing a fluorescent substance in a molar ratio of 50: 1. Then, the two types of liposomes were dialyzed at a temperature of 4 ° C for 24 hours using a 10 kDa dialysis membrane and then mixed at a ratio of 3: 7 (v-vesicle: t-vesicle) Methanol in the control group and snowberry extracts of Examples 1 to 4 of the present invention were added to the mixture containing ne 1a and VAMP-2 at a concentration of 40 μg / ml, respectively. Then, the fluorescence intensity was measured using a fluorescence intensity measuring instrument (Model: SpectraMax M2, manufacturer: Molecular Device). The results are shown in Fig.

In FIG. 1, since the fluorescence intensity means a membrane fusion phenomenon between SNARE proteins, a low fluorescence intensity indicates that the membrane fusion inhibitory effect is excellent. The black circle represents the path of the control reaction. As can be seen from FIG. 1, it was confirmed that SNOWBERRY extract had a lower fluorescence intensity than the control group, thereby inhibiting membrane fusion between SNARE proteins. In particular, SNOWBERRY extract (Example 3) The fusion inhibition effect was excellent.

The fluorescence intensity of the control group was set as a criterion and the fusional rate (%), which is determined by the relative fluorescence intensity ratio (%) in Snowberry extract, was calculated. The results are shown in Table 1 below.

Effect of snowberry extract on membrane fusion Name of sample Membrane fusion rate (%) Control (control) 100 Example 1 Snowberry extract (raw) 51 Example 2 Snowberry broom extract 48 Example 3 Snowberry extract 0 Example 4 Snowberry extract (salt) extract 53

As can be seen from the above Table 1, the group treated with Snowberry extract showed better membrane fusion inhibition effect than the control group without Snowberry extract, and the Snowberry extract of Example 3 was superior to other Snowberry extracts It was confirmed that the membrane fusion inhibition effect was remarkably excellent. Accordingly, the Snowberry extract of the present invention effectively inhibits the membrane fusion phenomenon between the SNARE proteins, thereby exhibiting excellent skin wrinkle-reducing effects.

[Test Example 2] Inhibitory effect of neurotransmitter on PC12 cells

PC12 cells were cultured in collagen coated plates (60 mm dish) in Ham's F12K media containing 10% fetal calf serum, 5% fetal bovine serum and antibiotics. After submerging the medium, 2 ml of PBS was added to the culture medium, and the cells were separated from the dish wall by pipetting, and the cells were collected by centrifugation at 1,000 xg for 5 minutes. The cells were then pipetted to disperse the cell pellet , And the cells were placed in a new culture plate and cultured in an incubator in which 5% CO ₂ gas was supplied at 37 ° C. The [ ³ H] -noreadrenaline used in the experiment was purchased from Amersham. After the cells were inhaled from the plate of PC12 cells, PBS was added and the cells were detached from the plate wall. The cell counts were measured with a hematometer and the cells were inoculated into a fresh medium at a concentration of 2 x ¹⁰ cells / ml. After 24 hours of the addition of noradrenaline assay buffer ^{([3 H] -NA, 1.5} μ Ci / ㎖) was introduced for 90 minutes at a carbon dioxide gas incubator. After the reaction, the buffer solution was removed and washed three times with PBS. The cells were then added to a fresh medium at a concentration of 10 μg / ml of Snowberry extract of Examples 1 to 4 of the present invention and reacted for 30 minutes. Verapamil was used as a positive control and treated in the same manner as the Snowberry extract. After the medium was removed, a high concentration of K ⁺ buffer was added. After incubation in a carbon dioxide gas incubator for 12 minutes, the supernatant was recovered to measure the release of [ ³ H] -nonadrenaline by a scintillation counter. It was confirmed by the above method that snowberry extract inhibited the release of dopamine derivative noradrenaline in PC12 cells. The ratio (%) of noradrenaline in each experimental group was calculated on the basis of high concentration of K ⁺ buffer. The results are shown in Fig.

As can be seen from FIG. 2, except for the Snowberry extract, the remaining Snowberry extracts effectively inhibited the release of neurotransmitters. The Snowberry extract of Example 3 showed the effect of inhibiting neurotransmitter release Respectively. Therefore, the Snowberry extract of the present invention can exert excellent skin wrinkle-reducing effect by inhibiting the release of neurotransmitter.

Hereinafter, formulation examples of the composition according to the present invention will be described, but the present invention is not limited thereto.

[Formulation Example 1] Nutritional lotion

The nutritional lotion containing the Snoferberry extract was prepared with the compositions shown in Table 2 below.

ingredient Content (% by weight) Example 3 5.0 Squalane 5.0 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

[Formulation Example 2] Flexible longevity

The softened lotion containing the Snowberry juice extract was prepared with the composition shown in Table 3 below.

ingredient Content (% by weight) Example 3 5.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PEG 12 nonyl phenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

[Formulation Example 3] Nourishing cream

Nutritive creams containing Snoferberry extract were prepared with the compositions shown in Table 4 below.

ingredient Content (% by weight) Example 3 5.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 PEG60 hardened castor oil 2.0 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

[Formulation Example 4] Massage cream

Massage cream containing Snowberry extract was prepared with the composition shown in Table 5 below.

ingredient Content (% by weight) Example 3 5.0 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 PEG60 hardened castor oil 2.0 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

[Formulation Example 5] Pack

A pack containing the Snowberry extract was prepared in the composition shown in Table 6 below.

ingredient Content (% by weight) Example 3 5.0 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonyl phenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount Purified water Balance Sum 100

1 is a graph showing an inhibitory effect of a membrane fusion phenomenon by Snowberry extract according to the present invention.

FIG. 2 is a graph showing the effect of inhibiting neurotransmitter release in PC12 cells by Snowberry extract according to the present invention.

Claims (4)

A cosmetic composition for improving skin wrinkles, comprising an extract of Snowberry treated with a collagen spraying method as an active ingredient. The method of claim 1, wherein the snowberry extract is a snowberry ethanol extract, A cosmetic composition for improving skin wrinkles. 3. The cosmetic composition for improving skin wrinkles according to claim 1 or 2, wherein the content of the Snowberry extract is 0.0001 to 30% by weight based on the total weight of the composition. The cosmetic composition for improving skin wrinkles according to claim 2, wherein the Snowberry ethanol extract is obtained by extracting with 80% ethanol.

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