KR101638170B1

KR101638170B1 - Plastic slide for preserving shape of the cell

Info

Publication number: KR101638170B1
Application number: KR1020150154085A
Authority: KR
Inventors: 김일희
Original assignee: (주)조아테크; 김일희
Priority date: 2015-11-03
Filing date: 2015-11-03
Publication date: 2016-07-08
Also published as: WO2017078406A1

Abstract

The present invention relates to a plastic slide which is easy to preserve cell shape, and which can reduce cost by injecting a slide with plastic, prevent breakage of impact, recycle the used slide as a plastic raw material, Preserve the steric form of the stained cells when storing the slides.

Description

[0001] The present invention relates to a plastic slide for preserving cell shape,

The present invention relates to a plastic slide which is easy to preserve a cell shape, and more specifically, to a method for reducing a cost by injecting a slide with plastic, preventing damage to the impact, recycling the used slide as a plastic raw material, To a plastic slide which enables to preserve the three-dimensional shape of the smear cell when storing the slide by forming a step.

Slides for diagnostic tests are rectangular shaped thin plates for testing the patient's pathology by smearing the cell fluid and are usually produced by processing glass. These slides are dependent on the total quantity of products to be manufactured.

Clinical pathology experiments and tests are based on the disposal of firearms as a result of bacterial infection, deformation of secondary cells, and contamination due to cell necrosis. In principle, slides made of glass are fossil raw materials and therefore secondary waste is generated.

In addition, since slides made of glass are made in a plane, in order to preserve the smear cells, in the process of covering with the cover glass, the three-dimensional shape of the cells is squeezed and the shape is not completely preserved.

On the other hand, Korean Patent Laid-Open No. 10-2015-0059218 and the like disclose the prior art relating to a plastic slide.

SUMMARY OF THE INVENTION The present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to inject a plastic slide with a step formed therein to preserve the stereoscopic shape of a cell during storage of the slide.

According to an aspect of the present invention, there is provided a slide comprising: a slide having an upper surface and a lower surface; A smear face formed by a step formed on one side of the slide; And a plurality of gates are formed around the smear face in the form of a passage that diverges from the smear face in each direction.

Here, the upper surface and the lower surface are cut at the ends of each of the gates to form an absorbent insert slit, and the absorbent insert slit is inserted into the formed absorbent insert slit to absorb moisture of the cell liquid smeared on the smear surface.

The lower surface of each of the absorber insertion slits is formed with a predetermined angle and the reflection surface is formed so that the light irradiated from the lower end of the slide is refracted so as to concentrate on the smear surface.

Finally, a lamination groove is formed on the upper surface, and a lamination protrusion is formed on the lower surface of the lower surface of the lamination groove, so that a plurality of the slides can be stacked.

The plastic slide according to the present invention has the following effects.

First, the steric form of the stained cells is preserved. The plastic slide according to the present invention is formed by embedding an embossed surface on an upper surface thereof, thereby forming a step on the periphery of the embossed surface. This makes it easier to preserve the morphology of the cells compared to the prior art where the slides were simply planar. In other words, conventionally, when the cover is covered with a cover glass in order to preserve the cells on the slide after inspection, the cells smear on a simple plane are squeezed by the cover glass, resulting in collapse of the cells. However, in the present invention, when a cell is covered with a cover glass, a step is formed on the surface where the cells are smudged, so that the cell is not squeezed, so that the cell is not distorted and its shape is preserved.

Second, it is easy to determine where the cells are smearing and helps prevent the cells from getting stuck. As described in the first effect, in the present invention, the side on which the cells are smudged is depressed and a step is formed. Therefore, it is possible to precisely grasp the position where the test person smears the cells, and since the step is supported by the position of the smear cell, the cell does not move well even in the minute movement of the slide.

Third, concentrate the light. The slide according to the present invention is made of transparent plastic, and the lower surface of the slide is inclined at a predetermined angle in the direction of the smear face to form a reflecting surface. Therefore, when the light is irradiated at the bottom of the slide, the light is reflected on the reflection surface, so that the light is reflected and concentrated on the smear surface in a state of low loss of light. Therefore, Can be inspected.

Fourth, slides can be stacked and stored. The slide according to the present invention may have a lamination groove formed on the upper surface thereof and a lamination projection formed on the lower surface thereof, and the slide may be laminated when the lamination projection and the lamination groove are fitted. The height of these lamellar protrusions is sufficiently high that the underside of the slide does not touch the smeared cells, so that the inspector can stack laminated cells without worrying that the smeary cells are squeezed by the slides.

Fifth, the examiner alleviates glare when examining cells with a microscope. The slide according to the present invention is recessed and formed on the opposite side of the bottom surface of the bottom surface. Therefore, the light irradiated to the concave surface is dispersed to alleviate glare because it relaxes the direct irradiation of the examinee's eye.

Sixth, it is easy to discharge the liquid component (cell preservation solution) of the cell fluid. The absorption liquid is absorbed into the absorbing film by inserting the absorbing plate into the absorbing sheet insertion slit or the absorbing sheet insertion groove formed in the slide according to the present invention. Therefore, it is only required to insert the adsorbent and take out the adsorbed adsorbent that has been absorbed from the adsorbent insertion slit or the adsorption container insertion groove, so that the operation is simple and the liquid component is easily discharged and the discharge effect is excellent.

1 is a perspective view of a plastic slide according to a first embodiment of the present invention;
2 is a plan view of the plastic slide shown in Fig.
3 is a bottom perspective view of the plastic slide shown in Fig.
FIG. 4 is a side view of a plurality of plastic slides shown in FIG. 1 stacked. FIG.
5 is a view for explaining insertion of an adsorbent onto the slide shown in Fig. 1; Fig.
6 is a partial cross-sectional view schematically illustrating the absorption of a liquid component into an adsorbent in a state in which an adsorbent is inserted;
FIG. 7 is a schematic view illustrating the effect of light reflection on the concave surface under the smear and the effect of focusing light by the reflection surface formed on the slit structure in the plastic slide shown in FIG. 1;
8 is a schematic comparison of the shape of a cell when the cover glass is placed on a conventional slide and a slide according to the first embodiment of the present invention.
9 is a perspective view of a plastic slide according to a second embodiment of the present invention.
10 is a plan view of the plastic slide shown in Fig.
11 is a bottom perspective view of the plastic slide shown in Fig.
FIG. 12 is a view for explaining insertion of the adsorbent in FIG. 9; FIG.
13 is a cross-sectional view of the plastic slide shown in Fig.
14 is a partial cross-sectional view schematically illustrating the absorption of a liquid component into an adsorbent in a state in which an adsorbent is inserted;
15 is a sectional view of a plastic slide according to a third embodiment of the present invention;

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, some configurations which are not related to the gist of the present invention may be omitted or compressed, but the configurations omitted are not necessarily required for the present invention, and they may be combined by a person having ordinary skill in the art to which the present invention belongs. .

&Lt; Embodiment 1 >

Figure 1 is a perspective view of the plastic slide according to the first embodiment of the present invention, Figure 2 is a plan view of the plastic slide shown in Figure 1, Figure 3 is a bottom perspective view of the plastic slide shown in Figure 1, FIG. 5 is a view for explaining the insertion of the adsorbent into the slide shown in FIG. 1, and FIG. 6 is a view for explaining the insertion of the liquid FIG. 7 is a partial cross-sectional view for explaining the absorption of the component into the adsorbent. FIG. 7 shows the effect of light reflection along the concave surface below the smear surface in the plastic slide shown in FIG. 1, FIG. 8 is a view schematically showing the effect of concentrating the cover glass on the conventional slide and the slide according to the first embodiment of the present invention A view showing the comparison in the form of a capsule; Fig.

1 to 8, a plastic slide (hereinafter, referred to as a 'slide') that is easy to preserve cell shape according to the first embodiment of the present invention is formed in the form of a transparent thin plate, And a lower surface 140 are formed. The absorbent article insertion slit 160 and the fixing hole 170 are formed through the upper surface 110 and the lower surface 140.

The upper surface 110 is a part where the test person smears a cell fluid (a mixture of cells and a cell preserving solution) to absorb the liquid component (cell preserving solution), leaving only the cell 190. The upper surface 110 is formed with an embossing surface 115, a step 120, a gate 125, a lamination groove 130, and a data display 135.

The smear face 115 is formed by being embedded in a circular shape at the center of the upper surface 110 of the slide 1, Thus, the cells 190 are rounded along the smear face 115.

The step 120 forms a jaw along the circumference of the embedded smear face 115 to restrain the smear cell 190 from escaping from the smear face 115, The shape of the cell 190 is preserved when covering the cover glass 185 to preserve the cell 190.

The gate 125 is a passage through which the liquid component of the cell fluid dropped on the smear face 115 can flow out. A plurality of the gates 125 extend radially from the smear face 115 along the periphery of the step 120. In the first embodiment, eight gates 125 are formed, but the number of gates 125 can be increased or decreased according to practice.

The laminated grooves 130 are formed on the side of the upper surface 110 so that the rectangular shaped slides 1 can be stacked and stored in order.

The data display unit 135 is a unit for recording information of a person who smears the cell 190 on the slide 1.

Next, the lower surface 140 receives the light 180 irradiated from the lower end of the slide 1, and reflects and concentrates the light 180. A reflecting surface 145, a lamination protrusion 150, and a concave surface 155 are formed on the lower surface 140.

The reflection surface 145 is a surface where the outer portion of the portion where the absorbent insert slit 160 is formed on the lower surface 140 is inclined at a predetermined angle in the direction of the smear surface 115 and the light 180 ) To the smear face (115).

The lamination protrusions 150 are formed on the opposite side of the portion where the lamination grooves 130 are formed. 4, the stacked protrusions 150 may be inserted into the lamination grooves 130 of the other slides 1. The plurality of slides 1 may be formed by stacking the lamination grooves 130 and the lamination protrusions 150 They can be laminated and easily stored. In addition, the height of the lamination protrusion 150 is formed to be sufficiently high so as not to be in contact with the smear cell 190.

The concave surface 155 serves as a concave lens formed in a concave shape opposite to the smear surface 115 so as to spread the light 180 irradiated from the lower end of the slide 1 and spread evenly on the smear surface 115 And to prevent the light 180 from being directly irradiated to the eye of the examinee, thereby preventing the viewer's glare.

Next, the absorbent insert slit 160 is formed in a structure passing through from the top surface 110 to the bottom surface 140 around the smear face 115 at a predetermined area at the end of each gate 125. [ The absorbent 175 is inserted into the absorbent insertion slit 160 so that the liquid component of the cell liquid flows to the gate 125 and is finally absorbed into the absorbent 175. [

The fixing hole 170 is formed to hold the bar on the slide 1 that has been inspected. Thus, the slide 1 that has been inspected is put on the rod, and the laminated groove 130 and the lamination protrusions 150 are stacked one after another to be aligned with each other.

Hereinafter, a process of smearing the cells 190 on the slide 1 for microscopic examination will be described. First, as shown in FIG. 5, the examinee smears a cell fluid containing the cell 190 to be examined on the smear face 115 formed at the center of the slide 1. Thereafter, the suction nozzle 175 is inserted into the suction nozzle insertion slit 160 formed on the slide 1. 6, the liquid component flows along the gate 125 and is absorbed into the adsorbent 175 inserted into the adsorbent insertion slit 160 connected to the end of the gate 125. [ After the absorption, the adsorbent 175 is removed, and as a result, only the cells 190 remain on the smear surface 115. Thereafter, fixation solution 195 is applied onto cell 190 to fix smell 190 on the smear face 115. The fixation fluid 195 is placed on the cell 190 to block and seal the cell 190 from the outside air. The cell 190 is coated on the slide 1 and the fixing liquid 195 is coated on the slide 190. Finally, the cover glass 185 is placed on the cell 190 to complete the preparation of the cell 190. The inspector then places the slide (1) on the platform of the microscope and visually identifies the cell (190) through the objective lens.

Referring to Fig. 7, the light 180 is irradiated to the slide 1 fixed to the floor of the microscope. Illumination is placed under the ground floor to illuminate the light 180 with the slide 1 so that the inspector can focus the objective lens and inspect the cell 190 smeared on the slide 1. At this time, the light 180 is irradiated straight on the concave surface 155, but is dispersed while passing through the concave surface 155. Thus, the light 180 directly irradiated to the eyes of the examinee can be alleviated.

The reflective surface 145 formed on the lower surface 140 has an effect of focusing the light 180 spreading in various directions on the smear surface 115. That is, since the reflection surface 145 is inclined at a predetermined angle in the direction of the smear surface 115, the light 180 irradiated to the reflection surface 145 is distorted as shown in FIG. Accordingly, the spreading light 180 is gathered on the smear face 115 to reduce the amount of the light 180 to be lost and to concentrate the light 180, thereby making the cell 190 more visible to the examiner.

Referring to FIG. 8, the cells 190 'smudged on the conventional slide 1' and the cells smear (1 ') on the slide 1 according to the first embodiment of the present invention when the cover glasses 185' 190) are compared and explained. As shown in Fig. 8 (a), the conventional slide 1 'is simply made of glass as a plane. Therefore, when the cells 190 'are smudged and the fixing liquid 195' is applied and then the cover glass 185 'is placed to store the cells 190', the pressure of the cover glass 185 ' The cells 190 'smearing on the cells 190' may be squeezed and thus the cells 190 'may be distorted.

8 (b), the slide 1 according to the first embodiment of the present invention is formed by embedding the smear face 115 on which the cells 190 are smudged, and the smear face 115 The space between the cover glass 185 and the cell 190 is present. That is, since the cover glass 185 is supported by the step 120 when the cover glass 185 is placed to store the cell 190, the cell 190 and the fixing liquid 195 are supplied to the cover glass 185 And the shape is preserved without being squeezed.

&Lt; Embodiment 2 >

Figure 9 is a perspective view of the plastic slide according to the second embodiment of the present invention, Figure 10 is a plan view of the plastic slide shown in Figure 9, Figure 11 is a bottom perspective view of the plastic slide shown in Figure 9, FIG. 13 is a cross-sectional view of the plastic slide shown in FIG. 9, and FIG. 14 is a cross-sectional view illustrating a state in which a liquid component is absorbed into an absorbent Fig.

The second embodiment differs from the first embodiment in most of the configurations, but the shape and number of the absorbent storage insert slits 160 are different from the number of the gates 225 formed. That is, in the second embodiment, the suction pipe insertion groove 237 is formed on the upper surface 210 rather than the suction pipe insertion slit 160. In addition, in the second embodiment, the reflecting surface 145 for reflecting the light irradiated from the lower end of the slide 2 is not formed around the circumference of the concave surface 246. [ This is for absorbing the liquid component of the cell liquid easily by inserting the absorbing film 275 having a large area into the absorbing member insertion groove 237. Accordingly, the description of the same constitution as that of the first embodiment will be omitted.

9 to 14, in the slide 2 according to the second embodiment of the present invention, the number of gates 225 through which the liquid component flows out of the components of the cell fluid is 6, There are two absorber-insertion-insertion grooves 237 formed in the housing. Accordingly, three gates 225 are connected to the absorbent insertion grooves 237, respectively. In addition, the absorbent article insertion groove 237 is formed in a groove shape recessed only on the upper surface 210. As shown in FIGS. 12 and 13, in the same manner as in the first embodiment, the examinee drops the cell fluid on the smear face 215 and inserts the absorbent foam 275 into the suction pocket insertion groove 237, So that the liquid component of the cell liquid is absorbed into the adsorption film 275. After the absorption, the adsorbent 275 is removed to leave only the cells 190 on the smear face 215. The fixation liquid 195 is applied to the cells 190 to block the outside air, And the cell 190 is stored. Thereafter, the cells 190 are inspected by a microscope, and the slides 2, which have been inspected, are stacked by putting a rod in the fixing hole 260 and aligning the lamination protrusions 245 with the lamination grooves 230.

&Lt; Third Embodiment >

15 is a sectional view of a plastic slide according to a third embodiment of the present invention.

15, the slide 3 according to the third embodiment of the present invention has the same structure as that of the second embodiment except that the reflection surface 350 is additionally formed on the lower surface 340, to be. That is, all the configurations of the second embodiment, such as the smear face 315, the step 320, the absorbent article insertion groove 337, and the concave surface 346 formed on the lower surface 340 formed on the upper surface 310, The reflecting surface 350 is further formed around the concave surface 346 in the third embodiment. Therefore, when the slide 3 is placed on the microscope, the light 180 irradiated by the illumination at the lower end of the slide 3 is reflected on the reflection surface 350 and is directed to the smear surface 310. As a result, there is less loss of the irradiated light 180, and the light 180 is concentrated on the ointment surface 310, thereby allowing the examiner to see the slide 3 more clearly.

As described in detail above, the plastic slide 1, 2, 3 according to the present invention preserves the three-dimensional shape of the smear cell 190. The plastic slides 1, 2 and 3 according to the present invention are formed by embedding smearing surfaces 115, 215 and 315 on the upper surfaces 110, 210 and 310, At the periphery, stepped portions 120, 220, and 320 are formed. This makes it easier to preserve the morphology of the cells 190 compared to the prior art where the slides 1, 2 and 3 were simply planar. That is, conventionally, when the cover 190 'is covered with the cover glass 185' for preserving the cell 190 'on the inspected slide 1', the cell 190 'smoothed in a simple plane is covered by the cover glass 185' And the cells 190 'were crushed and crushed. However, in the present invention, when the cells 190 are covered with the cover glass 185, the cells 190 are formed on the smear faces 115, 215, So that the cell 190 is not distorted and its shape is preserved.

Further, it is easy to grasp the position where the cells 190 are smudged, and helps prevent the cells 190 from deviating from the smearing position. As described in the first effect, in the present invention, the smear faces 115, 215, and 315 on which the cells 190 are to be smudged are embedded, and the steps 120, 220, and 320 are formed. This allows the inspector to accurately grasp the position where the cells 190 are to be smudged and to ensure that even the microscopic movements of the slides 1, The cell 190 is not easily displaced to the position where the cell 190 is smudged.

It also concentrates the light. The slides 1 and 3 according to the present invention are made of transparent plastic and the lower faces 140 and 340 of the slides 1 and 3 are inclined at a predetermined angle in the direction of the smear faces 115 and 315, . The light 180 irradiated when the light 180 is irradiated from the bottoms of the slides 1 and 3 is reflected on the reflection surfaces 145 and 350 and concentrated on the smear surfaces 115 and 315. Accordingly, since the light 180 is not lost, the cells 190 smearing on the smear surfaces 115 and 315 can be more clearly inspected with improved brightness.

In addition, the slides 1, 2 and 3 can be stacked and stored. Lamination grooves 130 and 230 are formed on the upper surfaces 110 and 210 and 310 and lamination protrusions 150 and 245 are formed on the lower surfaces 140 and 240 of the slides 1 and 2 according to the present invention When the stacked protrusions 150 and 245 and the stacked grooves 130 and 230 are fitted together, the slides 1, 2 and 3 can be stacked. The height of the stacked protrusions 150 and 245 is sufficiently high that the lower surfaces 140, 240 and 340 of the slides 1, 2 and 3 do not touch the smear cell 190, It can be stacked without worrying about being pressed by the slides 1, 2, and 3.

In addition, when the inspector examines the cells 190 under a microscope, it alleviates glare. Slides 1, 2 and 3 according to the present invention are recessed on opposite sides of the smear faces 115, 215 and 315 of the lower faces 140, 240 and 340 to form concave faces 155, 246 and 346. Therefore, the light 180 irradiated by the concave surfaces 155, 246, and 346 is dispersed and alleviates glare because it alleviates direct irradiation of the examinee's eye.

Finally, it is easy to discharge the liquid component (cell stock solution) of the cell solution. The absorbent bags 175 and 275 are inserted into the absorbent sheet insertion slit 160 or the absorbent sheet insertion grooves 237 and 350 formed in the slides 1 and 2 according to the present invention, Is absorbed. Therefore, it is only required to simply remove the absorbent bags 175 and 275 and to remove the absorbent bags 175 and 275 from the absorbent sheet insertion slit 160 or the absorbent sheet insertion grooves 237 and 350. Therefore, And the discharge effect is excellent.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, And additions should be considered as falling within the scope of the claims of the present invention.

&Lt; Embodiment 1 >
1: Slide
110: Top surface
115:
120: step
125: Gate
130: laminated groove
135: Data display unit
140:
145: Reflecting surface
150:
155: concave surface
160: Absorbent insert slit
170: Mounting hole
175: Absorbent
180: Light
185: cover glass
190: cells
195: fixative
&Lt; Embodiment 2 >
2: Slide
210: Top surface
215:
220: step
225: Gate
230: laminated groove
235: Data display section
237: absorber insertion groove
240:
245:
246: concave surface
260: Mounting hole
275: Absorbent
&Lt; Third Embodiment >
3: Slide
310: Top surface
315:
320: step
337: absorber insertion groove
340:
346: concave surface
350: Reflecting surface
&Lt; Background Art &
1 ': slide
185 ': cover glass
190 ': Cell
195 ': fixative

Claims

The present invention relates to a plastic slide having an upper surface and a lower surface,
A step is formed on one surface of the plastic slide to form a recessed surface,
Wherein a plurality of gates extend radially from the smear face and are formed in the form of a passage around the smear face,
An absorbent insert slit is formed at an end of each of the gates through the upper surface to the lower surface,
The absorbent article insertion slit absorbs the liquid component of the cell fluid that is stuck on the smear face by inserting the absorbent foam,
Wherein the lower surface of each of the absorbent insert slits is formed with a reflective surface whose outer side is inclined by a predetermined angle so that light irradiated from the lower end of the slide is reflected on the surface of the plastic.

delete

The method according to claim 1,
Wherein a laminated groove is formed on the upper surface, and a lamination protrusion is formed on the lower surface of the lower surface of the plastic laminate on the opposite side of the laminated groove.