KR101511446B1 - A body hair growth inhibition composition comprising usnic acid as an effective ingredient - Google Patents

Abstract

The present invention relates to a composition for inhibiting growth of body hair comprising usnic acid as an effective component. The composition of the present invention inhibits cellular differentiation or proliferation of follicular cell, thereby inhibiting growth of hair. The composition of the present invention has no toxicity and side effects even if applied to a body, and is useful for removing or reducing hair of a tropical part.

Description

[0001] The present invention relates to a composition for inhibiting hair growth comprising Usnic acid as an active ingredient,

TECHNICAL FIELD The present invention relates to a composition for inhibiting hindlimb growth comprising uronic acid as an active ingredient. More specifically, the present invention relates to a cosmetic composition for inhibiting hindlimb hair growth, which comprises, as an active ingredient, ousnic acid or a cosmetically acceptable salt thereof. The present invention also relates to a pharmaceutical composition for inhibiting hindlimb growth comprising, as an active ingredient, a usnonic acid or a pharmaceutically acceptable salt thereof.

Hair is a generic term for hairs and hair, which develops from the skin and develops as a skin appendage. The hair repeats the anagen, ctagen, and talogens and has a cycle of growth, elimination, and initiation.

Hair is essentially an organ that has been functioning to protect the body but is degenerating with evolution. Human beings are covered with fur all but palms, soles, lips, and hairs, and the number is between 120 and 1.5 million. The hair is divided into hardness and softness by hardness. The moxa is black and hard with hair, eyebrow hair, eyelash hair, etc. The soft hair is soft and soft hair. In recent years, not only the coarse hairs on the side such as the armpit and the leg but also the hair on the whole body such as the human hair for the purpose of beauty are increasing.

Existing hair removal methods are largely divided into physical hair removing method (eg razor) and chemical hair removing method (eg hair removing cream), waxing or tweezers to remove the hair from the root, Have been used. These methods have the disadvantage that they cause irritation to the skin when used over a long period of time, as well as roughness of the skin, contact dermatitis and pigmentation, and the use thereof is limited for wound areas and some parts such as the face.

Therefore, there is a demand for development of a hair growth inhibitor that can overcome these drawbacks and at the same time show less hair irritation and show hair removal effect by utilizing the hair growth cycle and the differentiation principle of hair follicle cells.

Under these circumstances, the inventors of the present invention confirmed that Usnic acid inhibits cell differentiation and proliferation of hair follicle cells to inhibit growth of hair, and further confirmed that it has excellent hair removal effect when applied to human body, thereby completing the present invention Respectively.

The object of the present invention is to inhibit hair growth by using a composition containing uronic acid as an active ingredient.

Specifically, the present invention provides a composition for inhibiting hair growth, which has an effect of inhibiting the growth and proliferation of hair follicular cells by containing, as an active ingredient,

In order to solve the above-mentioned problems, the present invention provides cosmetic composition for hair growth inhibition comprising, as one embodiment, usnic acid or a cosmetically acceptable salt thereof as an active ingredient.

According to another embodiment of the present invention, there is provided a pharmaceutical composition for inhibiting hindlimb growth comprising, as an active ingredient, únic acid or a pharmaceutically acceptable salt thereof.

In another embodiment of the present invention, the Usnic acid is represented by the following general formula (1).

[Chemical Formula 1]

In another embodiment of the present invention, the Usnic acid is characterized by inhibiting the differentiation or proliferation of hair follicle cells.

In another embodiment of the present invention, the Usnic acid is characterized by inhibiting the expression of ornithine decarboxylase (ODC) gene.

In another embodiment of the present invention, the content of the usnic acid may be 0.0005 wt% to 5 wt% of the total composition.

In another embodiment of the present invention, the composition may have a formulation selected from the group consisting of liquid, oil, cream, ointment, stick, pack, pasta, powder, gel and spray, It does not.

Hereinafter, the present invention will be described in detail.

The present invention is based on the fact that Usnic acid has the effect of inhibiting the differentiation and proliferation of hair follicle cells.

The term " usnic acid "used in the present invention is preferably a compound represented by the following formula (1), and the name of the compound is 2,6-diacetyl-7,9-dihydroxy- Dimethyl-1,3 (2H, 9bh) -dibenzofuran dione.

[Chemical Formula 1]

Usnica diffracta of Lecanorales, a lichen species, And the like. It has been reported that Usnic acid inhibits melanin production and has an inhibitory effect on pigmentation and can be used as an effective ingredient of a cosmetic composition for skin whitening such as improvement of viscous or freckles. However, there is no study on the inhibitory activity of hair growth.

The Usnic acid of the present invention exhibits an effect of inhibiting differentiation or proliferation of hair follicle cells, and this effect was first identified in the present invention.

The hair follicle cell refers to a stem cell that produces hair existing in a hair follicle.

The term " differentiation of hair follicle cells " means a process in which hair follicles are formed by repeating cleavage and forming hard keratin.

The proliferation of the hair follicle cells means a process of increasing the number of cells by repeating the division of the hair follicle cells.

Specifically, in one embodiment of the present invention, it was confirmed that the hair follicle cell proliferation was inhibited when the composition containing the uronic acid as an active ingredient was treated.

In addition, the Usnic acid of the present invention has an effect of inhibiting the expression of ornithine decarboxylase.

The present inventors have found that polyamine plays an important role in the differentiation, division or proliferation of hair follicle cells, and that ornithine dicarboxylate is an enzyme necessary for biosynthesis of polyamines, and that the above-mentioned Usnic acid and Ornitine Dicar The relationship between the expression of the enzyme and the enzyme was studied.

Thus, in one embodiment of the present invention, when follicle dermal papilla cells were treated with uronic acid in a concentration-dependent manner, cell counting was performed by using Cell Counting Kit-8. As a result, Usnic acid inhibited the proliferation of follicular cells in a concentration- (Example 1).

In another embodiment of the present invention, the cells expressing ornithine dicarboxylate are expressed in a concentration-dependent manner, followed by reverse transcription polymerase chain reaction (RT-PCR) As a result, it was confirmed that Usnic acid inhibited the expression of ornithine decarboxylase in a concentration-dependent manner (Example 3).

Inhibiting the expression of the enzyme preferably means reducing the expression of the enzyme at the transcription level.

In addition, in one embodiment of the present invention, it was confirmed that when ouzic acid was manufactured into ointment for external use or cream formulation and applied to human skin, excellent hair growth inhibitory effect was exhibited (Examples 4 and 5).

Therefore, the Usnic acid of the present invention has an effect of reducing the production and growth of new hair follicles, so that it can be usefully used as an effective ingredient of the hair growth inhibitor.

Accordingly, the present invention also provides a use of urosonic acid for the production of a cosmetic composition for inhibiting hair growth or a pharmaceutical composition for inhibiting hair growth, and a method for inhibiting hindlimb growth, which comprises applying an effective amount of urosonic acid to the skin.

The Usnic acid of the present invention may include uronic acid hydrate, uronic acid derivative and the like within the range having the same effect, and may include a solvent compound or a stereoisomer thereof. The Usnic acid may be chemically synthesized or extracted directly from natural materials such as Songra, or may be purchased commercially. When the above-mentioned usnonic acid is separated from a natural substance, it may be a concept including both an extract of a natural product or a fraction thereof, as long as it contains the above-mentioned usnonic acid.

Usnic acid used in one embodiment of the present invention was purchased from JHE JIANG YIXIN PHARMACEUTICAL CO., LTD.

The Usnic acid of the present invention can be used in the form of a pharmaceutical or cosmetically acceptable salt thereof.

As such salts, acid addition salts formed by pharmaceutical or cosmetically acceptable free acids are useful. Acid addition salts can be prepared in a conventional manner, for example, by dissolving the compound in an excess amount of an aqueous acid solution and precipitating the salt with a water-miscible organic solvent such as methanol, ethanol, acetone or acetonitrile. It is also possible to heat an equimolar amount of the compound and an acid or alcohol (e.g., glycol monomethyl ether) in water, and then evaporate the mixture to dryness, or the precipitated salt may be subjected to suction filtration.

As the free acid, an inorganic acid or an organic acid can be used. The inorganic acid may be, for example, hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, tartaric acid, etc. These may be used alone or in admixture of two or more. Non-limiting examples of the organic acid include methanesulfonic acid, p-toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, mandelic acid, The present invention also relates to the use of a compound of formula (I) as an acid, citric acid, lactic acid, glycollic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillyric acid, hydroiodic acid and the like. These may be used alone or in combination of two or more.

In addition, the Usnic acid can be made into a pharmaceutically or cosmetically acceptable metal salt using a base. The alkali metal or alkaline earth metal salt can be obtained, for example, by dissolving the compound in an excess amount of an alkali metal hydroxide or alkaline earth metal hydroxide solution, filtering the non-soluble compound salt, and evaporating and drying the filtrate. As the metal salt, it is preferable to produce sodium, potassium or calcium salt in particular, but it is not limited thereto. The corresponding silver salt can also be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (for example, silver nitrate).

The pharmaceutically or cosmetically acceptable salts of the above-described uonic acids may include all salts of acidic or basic groups that may be present in the compounds of the uronic acids, unless otherwise indicated. For example, the pharmaceutically or cosmetically acceptable salts may include sodium, calcium, and potassium salts of hydroxy groups, and other pharmacologically or cosmetically acceptable salts of amino groups include hardro-bromide, sulfate (Salicylate) salts such as hydrogen sulfates, phosphates, hydrogen phosphates, dihydrogen phosphates, acetates, succinates, citrates, tartrates, lactates, mandelates, methanesulfonates (mesylates) and p- And can be prepared through a method for producing a salt known in the art.

The term "hairs" used in the present invention refers collectively to hairs distributed in the body. The distribution of the hairs is not particularly limited. However, for the purpose of the present invention, it is preferable that the hairs are distributed in a body part which causes harm to aesthetics due to occurrence, growth, or excessive distribution of hairs such as armpits, arms, It can mean hair.

The term "hair growth inhibition" used in the present invention includes not only inhibiting hair growth or growth, slowing hair growth rate, but also reducing hair thickness or length.

The usnonic acid of the present invention can be preferably added to an external preparation for skin hair growth inhibition, but is not limited thereto.

In the present invention, the external preparation for skin hair growth inhibition may be a cosmetic composition or a pharmaceutical composition. When the external preparation for skin hair growth inhibition is formulated into medicines or cosmetics, it may contain known dermatologically acceptable excipients which act as a carrier for the active ingredient. When formulated as a medicament, the contents disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can be referred to. When formulating into cosmetics, International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995, which is incorporated herein by reference in its entirety. Which are incorporated herein by reference.

Specifically, the cosmetic composition containing the uraconic acid according to the present invention can be used as a cosmetically acceptable carrier, diluent, adjuvant, coloring agent, stabilizer, flavoring agent, surfactant, oil, humectant, alcohol, A pH adjuster and a sunscreen agent may be further added to the composition of the present invention and the composition of the present invention may be added to the skin such as a liquid, oil, cream, ointment, stick, pack, pasta, powder, It is also available as a formulation.

For the purpose of the present invention, the cosmetic composition may preferably be manufactured from semi-solid preparations such as external ointments, lotions and the like.

In addition, the pharmaceutical composition containing the urokinic acid or the pharmaceutically acceptable salt thereof according to the present invention can be used as a pharmaceutically acceptable carrier, diluent, adjuvant, colorant, stabilizer, flavor, interfacial agent, oil, It may further contain an alcohol, a thickening agent, an antioxidant, a pH adjusting agent and a UV blocking agent and may be formulated into a liquid, oil, cream, ointment, stick, pack, pasta, powder, But is not limited thereto. For the purposes of the present invention, the pharmaceutical compositions may preferably be formulated as semi-solid preparations such as external ointments, lotions, and the like.

The pharmaceutical composition of the present invention may be formulated into various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of such oral dosage forms include troches, lozenges, tablets, aqueous suspensions, oily suspensions, prepared powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs .

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose, or gelatin; Excipients such as dicalcium phosphate and the like; Disintegrating agents such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be used, and sweeteners, fragrances, syrups and the like may also be used. .

Furthermore, in the case of capsules, in addition to the above-mentioned substances, liquid carriers such as fatty oils can be further used.

Non-limiting examples of the parenteral preparation include injections, suppositories, respiratory inhalation powders, aerosol preparations for spraying, ointments, powder for application, oils, creams and the like.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, a sterilized aqueous solution, a non-aqueous solvent, a suspending agent, an emulsion, a lyophilized preparation, an external agent, and the like may be used. Examples of the non-aqueous solution and the suspension include propylene glycol, Polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like.

More specifically, when the pharmaceutical composition of the present invention is formulated into an injection, the composition of the present invention is mixed with water or a stabilizer or a buffer in water to prepare a solution or suspension, which is then mixed with an ampoule or a vial, And the like. When the pharmaceutical composition of the present invention is formulated into an aerosol formulation, a propellant or the like may be added together with the additive such that the water-dispersed concentrate or the wet powder is dispersed.

When the pharmaceutical composition of the present invention is formulated into an ointment, cream, or the like, it may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Zinc or the like as a carrier.

The route of administration and the mode of administration of the pharmaceutical composition of the present invention may be independent of each other and are not particularly limited in the method and may be arbitrarily administered and administered as long as the pharmaceutical composition can reach the desired site It is possible to follow the method. The pharmaceutical composition may be administered orally or parenterally.

The parenteral administration may be, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, and a method of applying, spraying or inhalation the composition to a diseased site may also be used But are not limited to.

The pharmaceutical composition of the present invention may preferably be administered parenterally, and more preferably, it may be administered in a manner such that it is applied to the skin.

Preferably, the ornicotin decarboxylase inhibitor, ornic acid, contained in the composition of the present invention is contained in a concentration of 0.0005 wt% to 5 wt% of the total composition. This is because it is difficult to obtain the effect at a concentration of less than 0.0005% by weight of the component and a problem of formulation stability may occur at a concentration of more than 5% by weight.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the age, sex, weight, symptom, degree of disease, drug form, administration route and period of time of the subject, but can be appropriately selected by those skilled in the art. However, for the desired effect, the pharmaceutical composition of the present invention is preferably administered at 0.01 to 1000 mg / day. The administration can be done once a day, or divided into several times. In addition, the dose may be increased or decreased according to age, sex, weight, degree of disease, route of administration, and the like. Accordingly, the dosage is not limited in any way to the scope of the present invention.

The present invention relates to a composition for inhibiting hair growth comprising Usnic acid as an active ingredient, and is excellent in the effect of suppressing the cell differentiation or proliferation of hair follicle cells and inhibiting hair growth at a desired site of hair removal.

In addition, the composition of the present invention is advantageous in that it can be appropriately formulated to remove or reduce hairs in a local area, and has no toxicity and no side effects even when applied to a human body.

FIG. 1 is a graph showing changes in cell proliferation after treating follicle dermal Papilla cells with concentration of uronic acid.
FIG. 2 is a graph showing changes in cell proliferation after treatment of uric acid by concentration in HT-29 cells. FIG.
FIG. 3 is a graph showing the relative expression of ornithine decarboxylase after treatment of uric acid by concentration in HT-29 cells using reverse transcription PCR (RT-PCR).

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.

Example  One: Usenic acid Hair follicle cell  Identification of differentiation and proliferation inhibitory efficacy

In this example, changes in cell division of Follicle Dermal Papilla cell (Promo cell) were measured by Cell Counting Kit-8 to examine the effect of Usnic acid on the differentiation and proliferation of hair follicle cells.

First, a uniform number of cells were plated on a 96-well plate, and then stabilized, and then treated with concentration of each of the cells (JHE JIANG YIXIN PHARMACEUTICAL CO., LTD., Hereinafter referred to as Usnic acid). Three days after treatment, changes in cell division were measured by Cell Counting Kit-8. Table 1 and Fig. 1 show the degree of change of non-treated cell division 3 days after treatment with Usnic acid. As a positive control, α-difluoromethylornithine (DFMO), which is known as ornithine decarboxylase inhibitor, was used. The degree of cell division in the following Table 1 is shown relative to the degree of cell division of the negative control group.

sample Negative control (no treatment) Positive control (DFMO treatment) Usnic acid Sample concentration 2.5 mM 5mM 2.5 ug / ml 5 ug / ml Relative degree of cell division 1.00 0.69 0.49 0.62 0.27

As can be seen from Table 1 and FIG. 1, Promo cells treated with Usnic acid showed cell division to decrease in a concentration-dependent manner.

Example  2: Usenic acid HT -29 cell cell division

In this example, to examine the effect of inhibiting the expression of ornithine decarboxylase in usnonic acid, a colon-derived adenocarcinoma HT-29 cell known to have a high expression of ornithine decarboxylase was used.

Based on the decrease in the expression of ornithine dicarboxylate enzyme, cell division was also measured by the MTT cell proliferation assay kit. A uniform number of cells were plated on a 96-well plate and stabilized by treatment with uronic acid. On the third day, the cells were reprocessed with uronic acid while replacing the cell culture medium. After 4 days of treatment with the uracil, the cell division was measured by MTT cell proliferation assay kit. The degree of cell-to-cell ratio change after 4 days of treatment with Usnic acid is shown in Table 2 and Fig. As a positive control, α-difluoromethylornithine (DFMO), which is known as ornithine decarboxylase inhibitor, was used. In Table 2, the degree of cell division was shown relative to the degree of cell division of the negative control group.

sample Negative control (no treatment) Positive control (DFMO treatment) Usnic acid Sample concentration 2.5 mM 5mM 2.5 ug / ml 5 ug / ml Relative degree of cell division 1.00 0.69 0.48 0.49 0.25

As can be seen from Table 2 and FIG. 2, HT-29 cells treated with Usnic acid showed a cell-division decrease in a concentration-dependent manner.

Example  3: Ornithine Digital Camera  Enzyme reduction investigation

As in the cell division assay, RNA was harvested 4 days after HT-29 cells treated with uronic acid and the relative content of ornithine dicarboxylase was determined after cDNA synthesis. Changes in the ornithine dicarboxylase enzyme were determined by reverse transcription-polymerase chain reaction (RT-PCR) in order to determine the mRNA sequence of ornithine decarboxylase from human genomic DNA. The primers used at this time are shown in Table 3.

order SEQ ID NO: ODC (forward direction) 5'-tgaccacgcacatgtaaagc-3 ' One ODC (reverse direction) 5'-cattgaacgtagaggcagca-3 ' 2 GAPDH (forward direction) 5'-cgagatccctccaaaatcaa-3 ' 3 GAPDH (reverse direction) 5'-ttcacacccatgacgaacat-3 ' 4

As a result of the experiment, it was confirmed that the relative mRNA amount of ornithine decarboxylase in the HT-29 cells treated with the uric acid was decreased by the treatment with the uronic acid. The results were analyzed using the Comparative C _T (ΔΔC _T ) Experiment and the internal control using GAPDH. The results are shown in FIG.

Hereinafter, the formulations of cosmetic compositions containing usnonic acid are shown by the production examples.

Manufacturing example  1 and Comparative Example  One: Usenic acid  Manufacture of ointment for external skin containing

(Osteogenic acid-containing ointment (Preparation Example 1) and ouncemic acid-free skin ointment (Comparative Example 1) were prepared in the composition shown in Table 4 below.

Composition Production Example 1 (% by weight) Comparative Example 1 Usnic acid
Diethyl sebacate
Nonsense
Polyoxyethylene oleyl ether phosphate
Sodium benzoate
vaseline 0.5
8
5
6
Suitable amount
to 100 -
8
5
6
Suitable amount
to 100

Manufacturing example  2 and Comparative Example  2: Usenic acid  Manufacture of cream containing

(Production Example 2) containing uronic acid and a cream containing no uronic acid (Comparative Example 2) were prepared with the composition shown in Table 5 below.

Composition Production Example 2 (% by weight) Comparative Example 2 Usnic acid
Stearic acid
ethanol
Potassium hydroxide
glycerin
Propylene glycol
antiseptic
incense
Purified water 0.2
15.0
1.0
0.7
5.0
3.0
Suitable amount
Suitable amount
to 100 -
15.0
1.0
0.7
5.0
3.0
Suitable amount
Suitable amount
to 100

Example  4: Investigation of hair removal effect

Twenty women aged 25 to 35 years who were healthy to the creams of Preparation Example 2 and Comparative Example 2 were subjected to the following hair removal effect.

Subjects applied the cream of Comparative Example 2, which had no Usnic acid on the left side, and the cream of Preparation Example 2, containing 0.2% of Usnic acid on the right side, to the legs twice a day for 2 months. After 2 months, the effects of hair removal were evaluated by questionnaire and image analysis. The subjects' questionnaires were three stages of no change, slight improvement, and significant improvement compared to before use with respect to changes in hair thickness and growth retardation. The results are shown in Table 6 below.

sample No improvement Slight improvement A significant improvement Comparative Example 2 14 6 0 Production Example 2 2 9 9

As can be seen from Table 6, when the production example according to the present invention was used, it was found that the effect of hair removal due to hair thickness change and growth delay was excellent.

Example  5: Body image analysis

For image analysis of body hair, subjects were instructed to shave their legs four days before evaluation. Evaluation was carried out before and after the experiment. The results of measurement of hair growth of Production Example 2 and Comparative Example 2 by image analysis are shown in Table 7 below by averaging the decreasing rate with respect to the hair length before the experiment.

Production Example 2 Comparative Example 2 Before experiment 1.00 1.00 after 2 weeks 0.72 (28% reduction) 1.00

As can be seen from Table 7, it can be seen that the hair growth retarding effect is significant when the preparation example of the present invention is supplemented with the uronic acid.

<110> LG HOUSEHOLD & HEALTH CARE LTD. <120> A body hair growth inhibition composition comprising usnic acid          as an effective ingredient <130> PA130925 / KR <160> 4 <170> Kopatentin 2.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> forward primer for ODC <400> 1 tgaccacgca catgtaaagc 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for ODC <400> 2 cattgaacgt agaggcagca 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> forward primer for GAPDH <400> 3 cgagatccct ccaaaatcaa 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer for GAPDH <400> 4 ttcacaccca tgacgaacat 20

Claims (12)

A cosmetic composition for inhibiting hair growth comprising usnic acid or a cosmetically acceptable salt thereof as an active ingredient.
The cosmetic composition for hair growth inhibition according to claim 1, wherein the Usnic acid is represented by the following formula (1).
[Chemical Formula 1]

The cosmetic composition for inhibiting hair growth according to claim 1, wherein the Usnic acid inhibits differentiation or proliferation of hair follicle cells.
The cosmetic composition for inhibiting hair growth according to claim 1, wherein the Usnic acid inhibits the expression of ornithine decarboxylase (ODC) gene.
[2] The cosmetic composition for inhibiting hair growth according to claim 1, wherein the content of the uronic acid is 0.0005 wt% to 5 wt% of the total composition.
The hair growth inhibitor according to claim 1, wherein the cosmetic composition has a formulation selected from the group consisting of liquid, oil, cream, ointment, stick, pack, pasta, powder, gel and spray. Cosmetic composition.
A pharmaceutical composition for inhibiting hindlimb growth comprising usnic acid or a pharmaceutically acceptable salt thereof as an active ingredient.
The pharmaceutical composition according to claim 7, wherein the Usnic acid is represented by the following formula (1).
[Chemical Formula 1]

The pharmaceutical composition for inhibiting hair growth according to claim 7, wherein the Usnic acid inhibits differentiation or proliferation of hair follicle cells.
The pharmaceutical composition for inhibiting hair growth according to claim 7, wherein the Usnic acid inhibits the expression of ornithine decarboxylase gene.
[Claim 7] The pharmaceutical composition according to claim 7, wherein the content of the uronic acid is 0.0005 wt% to 5 wt% of the total composition.
8. The method according to claim 7, wherein the pharmaceutical composition has a formulation selected from the group consisting of liquid, oil, cream, ointment, stick, pack, pasta, powder, gel and spray. A pharmaceutical composition.

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