KR101413186B1 - Feed composition comprising Neptheidae for Thamnaconus modestus and a breeding method therof - Google Patents

Abstract

The present invention provides a composition for the form of a male-domesticated feed comprising the genus Nephthiae as an active ingredient for the Thunnaconus modestus culture, and a method for cultivating the same.

Description

TECHNICAL FIELD The present invention relates to a composition for the feed of a filamentous fodder including feedstuffs of the genus Bombyx mori,

The present invention relates to a method form of Filefish (Thamnaconus modestus), more particularly, to a feed composition and form Filefish form Filefish method using the club containing sea cockscomb acids (Nephtheidae) as an active ingredient.

Recently, the fisheries industry has been shifting to fisheries centered on farming as the production volume of catches due to environmental pollution, reduction of fishing grounds, and overfishing of resources have reached the limit. In the case of Korea, aquaculture production has increased since 2000 and the aquaculture production has become the new main axis of the fishery industry since the aquaculture production has been ahead of general sea surface fishery production from 2006. In 2009, Respectively, to 1227,000 tons and 131,300 tons, respectively, accounting for 39% and 41% of total aquatic production.

Thamnaconus modestus is a fish belonging to the Tetraodontiformes rat dentist (Monacanthidae). It is distributed in the tropical and temperate waters of Japan, the South China Sea, and South Africa including Hokkaido and the coast of Korea. (Kim et al., Illustrated Korean Fishery Book and Teacher Education, 613pp, 2005). Rat dental fishes are 9 genera of 11 species in the country, and about 31 genera and 95 species are known all over the world (Kim et al., Korean Fisheries Book, Kyushu, 613pp, 2005). The demand for fish is high due to the high raw material consumption and raw meat quality of dried fish, and the resource amount is decreasing due to indiscriminate overfishing and environmental destruction (Ecology and fisheries of major fisheries of the National Fisheries Research and Development Institute, Rongcheng, 80 ~ 98pp, 1994).

Studies have been carried out to investigate the developmental process of egg-laying eggs and the morphogenesis of hatching fishes (Lee et al., Korean Journal of Fisheries, 12: 208-214, 2000), the reproductive cycle of barnacles in the southern coast of Jeju Science, 12: 208-214, 2000), Filefish digestive tract and digestive enzymes developed in accordance with the growth of jachieo (Kwok this, Korea Society of fish, 21: 149-157, 2009), genetic studies of Filefish (An et al , Genes & Genomics , 33: 499-504, 2011). In spite of being a commercially important fish species, there is a lack of research on ecological aspects in the world, and research on feeding ecology has been rarely done.

However, the conventional technology related to the mouse protein production has been mainly focused on the seed production, and as the feed used for the production, the phytoplankton such as chlorella or rotifer which is used for general fish production was used. This did not improve greatly.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a composition for aquaculture, However, these problems are exemplary and do not limit the scope of the present invention.

According to one aspect of the present invention, there is provided a composition for male-zoo fodder, comprising as an active ingredient a sea anemone family.

The feed composition may further comprise algae, Gastropoda or Thoracica, and the above-mentioned seaweed, gastropods or mangrove species may be contained in an amount of 1 to 30% by weight based on the total weight of the feed.

The seaweed may be at least one selected from the group consisting of green algae, brown algae, and red algae. For example, it may be kelp, seaweed, potato, and the like.

In addition, the gastropods may be at least one selected from the group consisting of Prosobranchia, Opisthobranchia and pulmonata subspecies, and may be, for example, ginseng spp. The gulls can be bullet gulls, gull gulls, parasitic gulls, snake gulls, double gull gulls, turtles, double gull gulls, double gull gull gulls, chestnut gulls,

In addition, the manganese species may be at least one selected from the group consisting of acrothoracic, thoracic, and rhizocephala. For example, it may be a barnacle, and may be a white barn, a harvest barn, a tender barn, a black barn, a high tall barn, a red barn, an octagonal barn, a barnacle barn or a barnacle barn.

The feed composition may further include feed materials conventionally used. The feedstock may include, for example, alfamia or rotifers.

The feed composition may further comprise a carrier acceptable to fish. The carrier may be a known carrier and may be added with various nutrients such as vitamins, amino acids, and minerals, an antioxidant, an antibiotic, an antibacterial agent and other additives according to need, and examples thereof include powders, granules, pellets, Suspension or the like. When the composition for aquaculture feed of the present invention is supplied, it can be supplied to the mouse alone or mixed with the feed.

In the present invention, analysis of stool contents of filefish appearing in the waters around Gyomun Island revealed that the fist, food organisms and feeding strategies by fist, Was found to be excellent. That is to say, the present inventors analyzed the feeding strategy of male mice and specifically proved that they specifically used the sea bream, marine algae and gastropods as food animals.

According to still another aspect of the present invention, there is provided a method of producing a blocky fish, which feeds the above-described composition for feed to a mouse.

In the above-described culture method, the file may be a native language or an adult language.

In the above-described culture method, the resveratrol additive may further contain algae or gastropods in the strains of sea bream, according to the standard height of the filefish.

In the above method, the composition for feed comprising the mackerel pike and the seaweed can be fed to the male fish and the feed composition including the male mackerel, the gastropod and the mackerel can be fed to the male fish .

According to one embodiment of the present invention as described above, a composition for aquaculture feed for a mouse chit style and a method for producing a mouse chit using the same can be implemented. Of course, the scope of the present invention is not limited by these effects.

FIG. 1 is a view showing the position of the sea area (34 ° 43.5 N, 127 ° 32.3E) around the Namhae Geomundo, which is collected from the family Thamnaconus modestus .
FIG. 2 is a graph showing cumulative prey curves of a documented mouse stomach.
FIG. 3 is a graph showing an eating behavior of a mouse according to an illustrative method. FIG.
FIG. 4 is a graph showing changes in stomach contents according to the growth of filefish. FIG.
FIG. 5 is a graph showing the mean number of prey per stomach (mN / ST) and the mean weight of prey per stomach per mound (mW / ST).

The terms used in this document are defined as follows.

As used in this document, "Gongbong Sea Bream" is a coral that lives on the sandy bottom of 5 ~ 100 m depth distributed on the southern coast including Jeju Island (Hong et al., Korean Marine Invertebrate Book, Academic Book, 479 pp, 2006 ). It is known that none of the species inhabited the Korean peninsula, which is relatively inferior in shape and structure to the mouth, and is not considered to be a favorite prey for common fish . The above-mentioned sea breams can be cultured through asexual reproduction, and cultured through fragmentation techniques. Segmentation is a way that corals can adapt and survive even in an algae-changing environment, which can be fragmented or created as a new colony as corals from the bottom attach to new substrates. This segmentation technique is known to be an important method for culturing corals (Highsmith, Marine Ecology - Progress Series, 7: 207-226 1982). Cut corals can be put in seawater, recovered, cultured on new substrates, and if transplanted without adequate recovery, they can be killed by cell damage or bacterial infection. The recovery period is about two weeks, but there may be differences depending on the external environment or species. Jungbong sea bream is one of the shortest growth rate and recovery period, and can be harvested if it is cultured for about 4 to 12 months. That is, since the breeding method is easy and the period is short, it can be easily applied to the feed composition.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It should be understood, however, that the invention is not limited to the disclosed embodiments and examples, but may be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, It is provided to fully inform the owner of the scope of the invention.

Example 1: Collection of Filefish

In this study, we used the new sea lure of Gyeongsang National University in June, August, October, and December 2011 (34 ° 43.5N, 127 ° 32.3E) 90 mm PE trawl fish (see Fig. 1). The monthly collection of the mice used in this study was 76 individuals in June, 21 in August, 24 in October, and 51 in December.

Experimental Example 1: Characteristic of a mouse

The collected filefishes were stored frozen and transported to the laboratory, and the standard length and weight of each individual were measured to 0.1 cm and 0.1 g, respectively. The length of the standard body was in the range of 16.2 to 39.1 cm, and the average body length was 22.4 cm, which was not significantly different from the monthly mean body length (Table 1).

EXPERIMENTAL EXAMPLE 2: Changes in Food Composition due to Growth of Filefish

2-1: Cumulative feeding curve - Sample size

Stomach contents of each subject were extracted and analyzed under a dissecting microscope. Cumulative prey curves were used to determine sufficient sample size for stomach contents investigation (Ferry and Cailliet, American Fisheries Society, p. 71-80, 1996). Analyzed food organisms were identified as Nephtheidae, Algae, Thoracica, Gastropoda, Desmospongia, Amphipoda, Brachyura, Ascidiacea, The above procedure was randomized 100 times by dividing into Echinoidea, Bivalvia, Cephalopoda, Hydrozoa, Phascolosomatidea and Actiniaria, Mean and standard deviation are shown in the graph.

As a result of analysis of the contents of stool samples of 172 mice, 65 stool samples showed no stool contents at 37.8%. The cumulative feeding curve of 107 individuals in which stomach contents were found was close to the asymptote in 76 individuals (see FIG. 2). At this time, the asymptote of the curve represents the minimum sample size for stomach content analysis. Therefore, the sample size in this study was sufficient to explain the stomach contents of the mouse.

2-2: Analysis of stomach contents

Stomach contents analysis showed the frequency ( % Fi ), wet weight ratio ( % W ), ranking index ( RI ), and food organism rank index ratio of each food organism. The above food bio index ratio is calculated as a percentage of RI (Song et al., Journal of the Korean Fisheries Society, 39: 42-48, 2006). In the following equations (1) to (3), Ai is the number of the filefish in which the corresponding food organism is found, N is the number of the filefish in which the contents are in the stomach, Wi is the wet weight of the food, Wtotal is the total food Means the wet weight of a creature.

Table 2 shows the results of food biochemical analysis of 107 individuals with stomach contents. The most important food organisms of the mouse species were Nephtheidae (Sepharidae), which had an appearance frequency of 23.2%, wet weight ratio of 51.2% and ranking index ratio of 68.3% Respectively. The next most important food creature is Algae, which has 14.1% of occurrence frequency, wet weight ratio of 13.9%, ranking index ratio of 11.3%, Algae of 12.6%, wet weight ratio of 7.6% and Thoracica ). Gastropoda, Desmospongia, Cephalopoda, and Amphipoda also appeared, but the amount was small. Therefore, it was found that the fish was an omnivore fish feeding on various benthos including the sea mackerel.

Experimental Example 3: Eating type and feeding strategy of male mice

Diagrams of stomach contents and feeding strategies were used to illustrate the feeding behavior of the mice. This method was shown by plotting the prey-specific abundance of a particular food organism against the frequency of occurrence (% F). Amundsen et al . (1996) proposed a graphical representation of the abundance of specific food organisms for frequency of occurrence, and many studies have been useful in analyzing fish feeding patterns and feeding strategies (Amundsen et al , J. Fish. Biol. , 48: 607614, 1996). The dominant prey of the food taxa in the upper part of the graph indicates that they are high-BPC feeding-specific species. In fish feeding strategies, feeding specific species have a narrow feeding width whereas feeding general species have a wide feeding width (Pianka, 1988) (see Pianka et al., Evolutionary Ecology , 4th ed. Harper Collins, New York, 468 pp, 1988).

The abundance of these specific food organisms was calculated using the following equation (4). To Equation 4, P _i is an abundance of specific food organisms in food organisms _i, S i is the weight of the food organisms i of gastric contents, S _ti feeding organisms i the whole of the contents above a feeding objects food organisms Weight .

As a result, as shown in FIG. 3A, the most important food creature of the mouse family was the clubbing sea bream. The mice were fed gastropods, sponges, cephalopods, and monocots, but showed low frequency of occurrence. In other words, it appeared to be a food specializing species that mainly feeds on the sea breams and seaweeds. Because it has a slower swimming ability than other fish, it is more likely that it feeds predatory fish, such as Macrura or Pices, , Which is considered to be a strategy to increase energy efficiency

Experimental Example 4: Changes in the composition of food according to the growth of the male mice

In order to investigate the changes in food biomass caused by growth, the collected samples were collected in four sizes (<21 cm, n = 34; 21-27 cm, n = 45; 27-33 cm, n = 22; , n = 6), and the composition of each food group was examined. To analyze the statistical difference of dominant prey biomass composition by body type, a kai test (x ² test) was performed.

Changes in stomach contents according to the growth of filefish are shown in FIG. 4 and Table 2, and stomach contents were significantly different according to body length (P <0.05). In the body below 21 cm, the swelling rate of the sea bream and seaweed were 41.0% and 16.3% respectively, and the hydrozoa showed the wet weight ratio of 8.7%. In the 21-27 cm body weight, the wet weight ratio increased to 49.2% and 18.0%, respectively, and the feeding rate of gastropod and desmospongia increased to 17.4% and 9.3%, respectively. In the 27 ~ 33 cm body, the wet weight ratio of pike sea bream and sea anemone increased to 62.1% and 13.4%, respectively, while seaweed tended to decrease to 12.4%. The wet weight ratio of Jongbong sea mackerel was decreased to 29.6% and the wet weight ratio of manganese was increased to 61.4%.

In summary, the larvae of the larvae of 21-27 cm and 27-33 cm were fed mainly to the barracuda and the sea bream, while the larvae of the birds of more than 33 cm increased the feeding rate. This suggests that the size of the mouth of a mouse does not change much in proportion to the increase in body size, and therefore does not resemble general fish food conversion.

Experimental Example 5: Relationship between the size of a mouse and the size of a food creature

Linear regression analysis was performed on the relationship between body size and feeding food size. As a result of the linear regression analysis, there was no significant difference in food biomass size (F = 0.123, P> 0.05).

The average weight of prey per stomach (mN / ST) and the mean weight of prey per stomach (mW / ST) , And the significance was tested using analysis of variance (ANOVA).

As a result, as shown in FIG. 5, the mean number of food per municipal population (mN / ST) (ANOVA, F1.105 = 4.829, P> 0.05) The mean weight (mW / ST) (ANOVA, F1.105 = 4.030, P <0.05) of food per body weight showed significant differences (see FIG. 5). The average food biomass per species was not significantly changed, but the average food biomass varied significantly, as the digestive organs including the stomach increased and the abdominal volume increased as the length increased .

Experimental Example 6: Overlapping of Feeding Organisms

Duplication of food-borne organisms was calculated using the dietary overap index using the following equation (5) (Schoener, Ecol., 51: 408-418, 1970). Here, Pxi and Pyi mean the wet weight ratio (% W) of the food organism i in the x and y groups. The degree of redundancy index value ranges from 0 to 1, and the closer to 1, the higher the degree of redundancy of food organisms. If the redundancy value is more than 0.6, it is considered to be significantly duplicated (Wallace, Am. Fish. Soc. , 110: 72-76, 1981).

As a result of using Schoener's index for food redundancy between the body genera, values between the body lengths of 21 cm and 21 cm and the body length of 21 ~ 27 cm were 0.66, 21 cm and 21 cm The value was similar to 0.62. The value between the 21-27 cm body group and the 27-33 cm body group showed the highest degree of redundancy as 0.82, and the body group over 33 cm showed an average value of 0.34 when compared with all body types, (Table 4).

&Lt; Formulation Example 1 > Preparation of composition for aquaculture feed

In accordance with one embodiment of the present invention, a feed composition was prepared with the following composition, using as an active ingredient, the mung bean sea bream, which was selected as a feed for male mice.

The other components such as lipase, tribasic calcium phosphate, vitamin E, enzyme powder, lactic acid bacteria, and Bacillus culture are added to the composition in the following composition. Next, the glucose is mixed so that the total weight becomes 100%, and the mixture is sufficiently mixed using a blender.

&Lt; Composition of composition for aquaculture feed >

Nephtheidae: 0.1 to 20% by weight,

Lipase: 0.001 to 0.01% by weight,

Tribasic calcium phosphate: 1 to 20% by weight,

Vitamin E: 0.01 to 0.1% by weight,

Enzyme powder: 1 to 10% by weight,

0.1 to 10% by weight of lactic acid bacteria,

Bacillus culture medium: 0.01 to 10% by weight, and

20 to 90% by weight of glucose.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (9)

A composition for the feed of Thalassa ( Thamnaconus modestus ) which contains Nephthiae as an active ingredient. The method according to claim 1,
Wherein the composition further comprises algae, Gastropoda or Thoracica. 3. The method of claim 2,
The composition of claim 1, wherein the algae, the gastropods or the hatchery are contained in an amount of 1 to 30% by weight based on the total weight of the feed. 3. The method of claim 2,
Wherein the seaweed is at least one member selected from the group consisting of kelp, sea bass and roots. 3. The method of claim 2,
Wherein the gastropods are at least one member selected from the group consisting of rodents. 3. The method of claim 2,
Wherein said bark is at least one selected from barnacles. 6. A method of filamentous fungal culturing comprising feeding the composition of claim 1 to a filamentous fungus. 8. The method of claim 7,
A method of filamentous filament formation, comprising feeding a composition for feed comprising filamentous sea anchovy and seaweed to a filamentous filament. 8. The method of claim 7,
Wherein the composition for feed comprising fish, mackerel, pelagic, and mangrove species is fed to a male fish.

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