KR101369449B1 - Mass production method of sinonovacula constricta's artificial seeds - Google Patents

Mass production method of sinonovacula constricta's artificial seeds Download PDF

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KR101369449B1
KR101369449B1 KR1020110118046A KR20110118046A KR101369449B1 KR 101369449 B1 KR101369449 B1 KR 101369449B1 KR 1020110118046 A KR1020110118046 A KR 1020110118046A KR 20110118046 A KR20110118046 A KR 20110118046A KR 101369449 B1 KR101369449 B1 KR 101369449B1
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larvae
seedlings
mother
spawning
clam
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KR1020110118046A
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KR20130053475A (en
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임여호
조주현
주우형
김상철
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김상철
전라남도
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The present invention relates to a method for mass production of artificial seedlings of garfish clam, wherein the salt concentration is adjusted to 22 ‰ in order to induce spawning of the mother's seedlings after selecting and washing the seedlings. A stimulating step 2 to 3 hours, a spawning step for adjusting the spawning environment in parallel with a low temperature stimulation to lower the water temperature 2 ~ 3 ℃, a hatching step for correcting the fertilized egg obtained in the spawning step, and hatched in the hatching step The seedling stage which grows larvae into 1mm of plaque, the intermediate breeding stage which grows the seedlings which passed through the depositor stage into about 1cm of aquaculture seedlings, and the seedling seedlings which passed the intermediate growth stage to the fishery Characterized by a spraying step.
According to an embodiment of the present invention, a large-scale production method of artificial seedlings of garfish clam can be provided at low cost by incubating a large number of fertilized eggs in parallel with a stimulus extraction and a low temperature stimulation which causes the mother to spawn artificially. During the larval rearing of the clam larvae, it is possible to raise a large number of high-efficiency seed plaques by increasing the survival rate of the larvae larvae by forming and breeding a mother-plate having a similar substrate to the natural environment.

Description

MASS PRODUCTION METHOD OF SINONOVACULA CONSTRICTA'S ARTIFICIAL SEEDS}

The present invention relates to a mass production method of artificial seedlings of garfish clam, and more specifically, artificially induces spawning at low temperature, artificially fertilizes garfish clam to be grown with seed plaques up to 1 cm. Seedling mass production method.

Garimat clams mainly inhabit coasts of Korea, Japan, China, etc., and are taxonomically belonging to the molluscs, Ichimok, and Garimat clams. Among the bivalve shellfish, the taste is caught after oysters, clams, and dongjuk. About 6,000 tons of fish are caught nationwide (Sukhyup 1995), but production has recently decreased sharply due to overfishing and environmental pollution. .

Garlic clams belonging to the high-quality shellfish are not only famous for their light taste, but they also clean up the coastal tidal flats of farming and contribute to improving the income of aquaculture fishers.

Garimat clam lives in the depth of 40 ~ 50cm in the inner ear, which is a sediment of nizil with high moisture content. The layer is exposed to 4 ~ 5 hours in the intertidal zone, and has excellent quality in Jeonnam area. It is distributed in Suncheon and Beolgyo areas.

Growth grows to 7cm per year and 9cm for 2 years, and relatively fast. The main spawning season of the southern coastal garfish is August to October, and the main spawning season is September. Garimat clam matures into mother's head only one year after infiltration and spawns in earnest.

Until now, the number of the clams was maintained due to natural spawning, but in recent years, the population has been rapidly decreasing due to environmental pollution, and it is urgent to expand and preserve the resources by increasing the population by the production of large-scale artificial seedlings.

However, recent domestic research has been conducted to investigate the changes in biochemical composition of some tissues according to the development of the gonad clam (Gunsan University, 2006), and the histological study on germ cell formation and the reproductive cycle of Jeju clam (1990, Cheju National University) "," Growth and Bioproduction of Ganseok Jisan Books in the Incheon Coast "(1994, Inha University)", "Growth and Production of Shellfish Shells (1997, Seoul National University)" Basic artificial seedling production technology, which is limited to basic histological research and is absolutely necessary for the increase of the population of the garfish clam by increasing the population, has not been attempted in Korea yet.

The present invention is to improve the conventional problems as described above, artificially induce spawning of the garfish clam artificially, mass production of artificial seedlings of the garfish clam breeding with seed seeds up to 1cm The purpose is to provide a method.

The artificial seedling mass production method of the garnish clam of the present invention for achieving the above object, first, to prepare a mother's nest shell washing and stabilizing the selected clam shell, and to control the spawning environment to induce spawning of the prepared mother's shell Egg stage, fertilization stage to fertilize the egg obtained in the stage of fertilization, flotation stage to float the larvae hatched in the incubation stage, deposition stage to grow the larvae in the stage of flotation to about 1mm of plaque, and deposition stage It features a method of mass production of artificial seedlings of Gari-mat shellfish, including the intermediate growth step of growing the coarse pulp into a culture seedling of about 1cm, and the spraying step of spraying the seedling for the culture that passed the intermediate growth step to the fishery.

As described above, according to the method of mass production of artificial seedlings of the garfish clam of the present invention, the artificial seedling mass of the garfish clam at low cost by hatching by obtaining a large number of fertilized eggs in parallel with the extraction stimulus and low temperature stimulation in order to artificially spawn the mother It can provide a production method.

In addition, during the larval rearing of the garfish clam, it provides a method of mass breeding a high-efficiency seed paddle which has raised the survival rate of the garfish clam shellfish by 10 times higher than that of the conventional land culture farm by forming and breeding a mother board having a breeding substrate similar to the natural environment. It is effective.

1 is a step-by-step flowchart of a method for mass production of artificial seedlings in a garfish clam according to an embodiment of the present invention.
Figure 2 is a photo of the main growth stage gari clam of Figure 1;
Figure 3 is a view showing the growth of the middle of Figure 1;

Hereinafter, a method for mass production of artificial seedlings in a garfish clam according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings and photographs.

As shown in the flow chart of Figure 1, the characteristics of the artificial seedling mass production method of the garfish clam of the present invention, first, the mother's wool preparatory step (S1) for washing and stabilizing the selected mother clam mother's cap and the mother's cap preparing step ( S1) a spawning step (S2) for adjusting the spawning environment to induce spawning of the mother's hair and the hatching step (S3) for correcting the selenium obtained in the above-mentioned spawning step (S2), and hatching in the hatching step (S3) Floating step S4) to float a larva, the deposition stage (S5) of growing the larvae of the floating stage (S4) to about 1mm of the length of the plaque, and the chipping through the deposition stage (S5) of about 1cm The intermediate growth step (S6) to grow into a seedling for farming, and the spraying step (S7) for sprinkling the seedling for farming past the intermediate growth step (S6) to the fishery.

The mother-preparation step is characterized in that the mother-in-law shellfish that has reached maturity is washed with filtered seawater and then stabilized for one day while carrying out flowing water.

In the egg-scattering step, in order to induce spawning of the mother's hair, a low temperature stimulus of 2 to 3 ° C. and an intermittent stimulus of 2 to 3 hours are performed at a water temperature of 24.5 ° C., and the salt concentration is maintained at about 22 ‰, creating a spawning environment. Features steps

The hatching step is a fertilization step of agitating the eggs obtained in the egg laying step in a water tank and then mixing 100,000 sperm of sperm for 10 to 20 minutes to fertilize the fertilized eggs, 50-70 Further, the method further comprises a seborrification step of removing excess sperm by filtration with a mesh such as a Muller gauze having a mesh of about μm, and an incubation step of allowing the fertilized egg which has undergone the seranization step to a density of 20 or less per ml until hatching. It is characterized by.

Table 1 shows the reference time by subdividing the time required by the development process of the garfish clam.

(Table 1) Required time by the development process of girly clams

Figure 112011089499870-pat00001

Referring to the embodiment of the present invention with reference to the picture of Figure 2, as shown in the picture of Figure 2-1 purchases garrison clam inhabited in Yongdueochon-si, Suncheon-myeon, Suncheon-si from August to 10 ~ 20kg Was used.

As shown in the photograph of Fig. 2-2, the mother's head was washed with filtered seawater filtered with a filter, and the seawater was allowed to flow for about one day before spawning was induced.

It is most effective to induce spawning at low temperature of 2 ~ 3 ℃ and extraction stimulation of 2 ~ 3 hours at the water temperature of 24.5 ℃, and keep the salinity concentration around 22 ‰. I have to decide. The amount of eggs varies depending on the size and condition of the garfish clam, but approximately 300,000 fertilized eggs per horse can be obtained.

For fertilization, 100,000 sperm of sperm are required, and the mixture of fertilized eggs and sperm in a 1-ton tank with air bubble injection (air) is allowed to stand for 10 to 20 minutes.

When the fertilized egg is confirmed, it is collected by Muller gauze of 50 ~ 70㎛ to remove foreign substances and remove excess sperm. When the eggs are finished, put the fertilized eggs in a plastic container of about 100ℓ at a density of 20 or less per ml, and leave the fertilized eggs until hatching.

The gonads are milky white for males and white for males. When spawning occurs approximately 3-4 hours after spawning, 9 hours after fertilization, the eggs are hatched into phase D larvae as shown in the photograph of FIGS.

In the floating step, the disinfection step of disinfecting fine filaments filtered through the mesh for 10 minutes at 80 ° C. or more for 10 minutes to provide the bottom quality for infiltrating the larvae, and after the disinfection step was immersed in fresh water for 2 days, 2 3 manufacturing step to dry for 3 days, breeding jersey composition step to spread the breeding jersey made by mixing the fine ocher 6: 6 in the 뻘 manufacturing step after 1 manufacturing step, and infiltrating the larvae in the breeding jersey 7 days while replacing the breeding larvae by replacing the breeding larvae, including the breeding larvae once every two days, so that the larvae infiltrated into the breeding larvae do not cause a disease such as ciliary worms due to the decay of the dead bodies or food wastes. Characterized in that it further comprises a floating step.

The larvae are fed by mixing 70% of Isochrysis species, 20% of Tetraselmis species, and 10% of Ketoceros species. Feed 50,000 pieces / ml.

Feeding larvae during the deposition phase was mixed at a rate of 70% of Isochrysis species, 20% of Tetraselmis species, and 10% of Ketoceros species, 60,000 to 100,000 / ml per day It is characterized in that it further comprises a deposition stage for feeding 7 days at a feeding density of the breeding to 1mm of the length of each.

Larvae in the deposition phase are about 170 μm after one week of incubation, but reach 250 μm in 15 days, and grow up to 500 μm in 30 days. The length of time up to 1mm of each sheet is different depending on the water temperature and environmental conditions, but it takes about 40 days. Up to 1mm, it has a shape similar to that of a shellfish, but grows around each sheet as it grows.

Photo 4 of FIG. 2 shows a small penetrator, photo 5 is a medium penetrator, photo 6 is a large penetrator, photo 7 is an invader larvae that crawls, and photo 8 shows a submerged chip (1 mm).

Intermediate growth step, the step of transferring the toothpaste grown to 1mm in length in the deposition stage to the natural farm, and in order to infiltrate the toothpaste transferred to the natural farm in the ear 40cm wide (1) and 20cm wide A platelet forming step of making a drainage passage 2, a patch spreading step of spreading the plaque evenly on the ridges of the formed platen, and a chipboard sprayed on the platen so as to easily infiltrate without being lost when the chipboard sprayed on the bed is high It further comprises the step of sprinkling the sprinkling water for sprinkling the sprinkles, and the intermediate growth step of breeding the chipped submerged into the bed plate for 1 month to seed 1cm.

(Intermediate development step example)

Since September, a part of the garfish clam farm is divided into circumferential plates using a board with a width of 10 X 20 m as shown in FIG. Ten thousand were sprayed onto the bedside.

The method of spraying was carried out at low tide, and spread it on evenly prepared intermediate farm bed plate, and then sprinkled with soft water on it to make it easier to infiltrate and prevent loss.

As shown in (b) of FIG. 3, the inner side of the mother board made a mockup 1 having a width of about 40 cm, and the drainage passage 2 was made of 20 cm to provide good drainage during low water.

After about two months, the chippings infiltrated into the mother bed grew into seed pads of about 1 cm and was sprayed on the whole fishery. Survival rate exceeding 50% was obtained by spraying 1mm tooth plaque, which showed 10 times survival rate compared to 5% survival rate when grown to 1cm in terrestrial culture. .

1: mockup 2: drainage

Claims (7)

In the artificial seedling mass production method
A mother-preparation step of washing and stabilizing the selected clam chicks;
And chaeran step of adjusting the spawning environment in order to induce spawning of the mother's mother's preparatory step is completed,
A hatching step of correcting the seran obtained in the chaeran step,
A floating step of floating the larvae hatched in the hatching step;
And a deposition stage for growing the larvae of the floating stage into a chip of about 1 mm in length,
And intermediate growth step of growing the chip through the deposition stage to a culture seedling of about 1cm,
Including the spraying step of sprinkling the seedlings for farming after the intermediate growth step in the fishery,
The floating step is a 뻘 disinfection step of sterilizing the fine 른 filtered through the mesh for 10 minutes at 80 ℃ or more to provide a substrate for the larvae immersed in larvae,
뻘 manufacturing step of immersing the 뻘 disinfection step in fresh water for 2 days and then drying for 2-3 days,
The breeding substrate composition step of spreading the breeding substrate made by mixing the fine ocher 6: 6 after the 뻘 manufacturing step to the bottom about 1cm,
Infiltrating larvae into the breeding substrate;
Gary larvae infiltrated into the breeding substrate is a galley, characterized in that it further includes a floating step for 7 days while replacing the quality by returning the entire quality, including the poor quality once every two days, so as not to cause diseases caused by the decay of the dead body or food waste Mass production method of artificial seedlings of taste shells.
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KR1020110118046A 2011-11-14 2011-11-14 Mass production method of sinonovacula constricta's artificial seeds KR101369449B1 (en)

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CN103314909A (en) * 2013-07-01 2013-09-25 江苏省淡水水产研究所 Method of breeding Corbicula fluminea on lake bed
CN103766258B (en) * 2014-01-26 2016-01-20 大连海洋大学 The artificial breeding method of a kind of mottle clam Ruditapes variegata
CN104855320B (en) * 2015-06-08 2017-05-31 宁波大学 A kind of method for improving hang razor clam cultured output and quality
CN105010198A (en) * 2015-08-10 2015-11-04 苏州市相城区阳澄湖镇剑成水产生态养殖专业合作社 Mixed high-yield culture method for razor clams
CN106665514A (en) * 2015-11-10 2017-05-17 高其和 Razor clam capture machine
KR101632849B1 (en) * 2015-12-31 2016-06-23 전라남도 Spat collection method of Scapharca subcrenata

Citations (3)

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Publication number Priority date Publication date Assignee Title
KR20100023336A (en) * 2008-08-21 2010-03-04 김경순 Culturing method for burrower bivalve
KR100953994B1 (en) * 2009-10-08 2010-04-21 김재민 Method of producing tegillarca granosa seedling
KR20110107712A (en) * 2010-03-25 2011-10-04 이정호 The bio-ceramic block with bentonite

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100023336A (en) * 2008-08-21 2010-03-04 김경순 Culturing method for burrower bivalve
KR100953994B1 (en) * 2009-10-08 2010-04-21 김재민 Method of producing tegillarca granosa seedling
KR20110107712A (en) * 2010-03-25 2011-10-04 이정호 The bio-ceramic block with bentonite

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