KR101235702B1 - methode for analyzing iron ion conentration in hydroponic nutrients and kits analyzing iron ion conentration in hydroponic nutrients - Google Patents
methode for analyzing iron ion conentration in hydroponic nutrients and kits analyzing iron ion conentration in hydroponic nutrients Download PDFInfo
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- KR101235702B1 KR101235702B1 KR1020110054129A KR20110054129A KR101235702B1 KR 101235702 B1 KR101235702 B1 KR 101235702B1 KR 1020110054129 A KR1020110054129 A KR 1020110054129A KR 20110054129 A KR20110054129 A KR 20110054129A KR 101235702 B1 KR101235702 B1 KR 101235702B1
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
본 발명은 양액재배의 무기 영양소 진단을 위한 간이 측정기 개발과 관련된 양액 내의 철 농도 분석방법 및 분석용 키트에 관한 것으로, 더욱 구체적으로는 시약에 측정하고자 하는 철 이온이 포함된 양액을 첨가하여 일어나는 색 변화에 대하여 흡광도를 측정하거나 비색표에 의한 대조를 통해 양액 내의 철 이온 농도 분석에 관한 것이며, 상기 시약으로 1,10- 페난트롤린(Phenanthroline) 과 염기(base)의 혼합물을 사용하는 양액 내의 철 이온 농도를 측정하기 위한 양액 내의 철 이온 농도 분석방법 및 이에 사용되는 분석용 키트를 그 특징으로 한다. 상기 염기(base)는 바람직하게는 탄산수소나트륨(Sodium Bicarbonate)이다.
본 발명에 의한 양액 내의 철 이온 분석용 키트는 간편한 방법으로 채취한 양액 내의 철 이온 농도를 정확하게 측정할 수 있도록 한 것이다.The present invention relates to a method for analyzing iron concentration in a nutrient solution and a kit for analysis related to the development of a simple measuring device for the diagnosis of inorganic nutrients in nutrient cultivation. It relates to the analysis of iron ion concentration in nutrient solution by measuring the absorbance for changes or by colorimetric control, and using iron in nutrient solution using a mixture of 1,10-phenanthroline and base as the reagent. Characterized by the iron ion concentration analysis method in the nutrient solution for measuring the ion concentration and the analysis kit used therein. The base is preferably sodium bicarbonate.
The kit for analyzing iron ions in the nutrient solution according to the present invention is to enable to accurately measure the iron ion concentration in the nutrient solution collected by a simple method.
Description
양액재배는 통상 흙을 사용하지 않고 작물의 생육에 필요한 양분을 함유한 배양액(이하, '양액'으로 약칭함)으로 재배하는 것으로서, 토양에 구애되지 않고 어디에서나 작물을 재배하기 위하여 개발된 것이며, 일반적인 토경재배에 비하여 높은 생산성과 우수한 품질의 작물을 재배할 수 있는 장점으로 인해 널리 사용되고 있다.Nutrient cultivation is usually cultivated with culture medium containing nutrients necessary for the growth of crops (hereinafter, abbreviated as 'nutrient solution') without using soil, and was developed to grow crops anywhere without being bound to soil. It is widely used due to the advantages of high productivity and high quality crops compared to general soil cultivation.
본 발명은 양액에 포함된 무기 영양소 중 하나인 철의 이온 농도를 분석하는 간편한 방법에 관한 것으로, 1,10-페난트롤린(Phenanthroline)과 염기(base)를 혼합하여 제조된 시약에 측정하고자 하는 철 이온이 함유된 양액을 첨가하여 일어나는 색변화를 이용하여 비색표 및 흡광도 대조를 통해 양액 내의 철 이온 농도를 확인하는 양액 내의 철 이온 농도 분석방법 및 분석용 키트에 관한 것이다.The present invention relates to a simple method for analyzing the ion concentration of iron, one of the inorganic nutrients contained in the nutrient solution, and to measure in a reagent prepared by mixing 1,10-phenanthroline and a base. The present invention relates to an iron ion concentration analysis method and kit for analyzing iron ions in a nutrient solution by using a color change generated by adding iron ions.
용액 중에 포함된 철 이온을 분석하는 방법으로는 주로 기기분석이 이용되며, 흡광도 측정법도 수질공정시험법에 기재되어 있다. 수질공정시험법에는 첫째로 원자흡광광도법(AA), 흡광광도법(페난트로린 법) 및 유도결합플라스마 발광 광도법 (ICP)법이 있으며, 이러한 과정에서 시료의 정확한 측정을 위해서는 다음과 같은 시료 전처리 과정을 거쳐야한다. 이는 시료 중의 총 철 함량을 구하기 위한 절차이다.Instrumental analysis is mainly used to analyze iron ions contained in the solution. Absorbance measurement is also described in the water quality test method. Water quality test methods include Atomic Absorption Spectroscopy (AA), Absorption Spectroscopy (Phenantroline), and Inductively Coupled Plasma Emission Spectroscopy (ICP). Should go through This is a procedure for determining the total iron content in the sample.
상기 시료 전처리 과정을 보다 상세히 설명하면 하기와 같다. 철의 함유량이 미량인 경우에는 시료 100 ㎖를 취하여 질산 2 ㎖를 넣고 끓인 다음 암모니아수(1+1)를 넣어 약알칼리성으로 하고 수분간 계속 끓여서 침전을 생성시키고 정치한다. 침전을 여과하여 온수로 수회 씻은 다음 침전을 원래 비커에 소량의 물로 씻어서 넣고 염산(1+1) 4 ㎖를 넣고 가열하여 녹인다. 이 용액을 앞의 거름종이를 사용하여 여과하면서 거름종이에 남아 있는 수산화제이철을 녹이고 온수로 거름종이를 씻어준 다음 여액과 씻은 액을 합하여 일정량으로 한다. 용해성 철을 측정할 경우에는 시료채취 즉시 여과하고 여액을 상기 전처리 방법에 따라 전처리하여 시료용액으로 한다.The sample pretreatment will be described in more detail below. If the iron content is very small, take 100 ml of the sample, add 2 ml of nitric acid, boil it, add ammonia water (1 + 1) to make it slightly alkaline, and continue to boil for several minutes to form a precipitate. The precipitate is filtered off and washed several times with warm water. The precipitate is washed with a small amount of water in the original beaker, and 4 ml of hydrochloric acid (1 + 1) is added to dissolve by heating. The solution is filtered using the previous filter paper to dissolve the ferric hydroxide remaining in the filter paper, wash the filter paper with warm water, and add the filtrate and the washed solution to a certain amount. When soluble iron is measured, the sample is filtered immediately and the filtrate is pretreated according to the pretreatment method to prepare a sample solution.
한편, 공정시험법 상에서 손쉽게 이용할 수 있는 흡광광도법은 하기의 절차에 의한다. 상기 전처리한 용액을 처음의 거름종이로 여과하여 거름종이에 붙어 있는 수산화제이철을 녹여내고 온수로 수회 씻어서 여액과 씻은 액을 100 ㎖ 용량플라스크에 옮긴다. 물을 넣어 액량을 약 70 ㎖로 하고 염산히드록실아민 용액(10 W/V %) 1 ㎖를 넣어 흔들어 섞는다. o-페난트로린용액(0.1 W/V %) 5 ㎖를 넣어 흔들어 섞고 초산암모늄용액( 50W/V % ) 10㎖를 넣어 흔들어 섞은 다음 실온까지 식힌다. 물을 넣어 표선까지 채워 흔들어 섞은 다음 20 분간 방치하여 시료용액으로 한다. 따로 물 50 ㎖를 취하여 시료의 시험방법에 따라시험하여 바탕시험액으로 한다. 바탕시험액을 대조액으로 하여 층장 10 ㎜ 흡수 셀에 옮겨 510 ㎚에서 시료용액의 흡광도를 측정하고 미리 작성한 검량선으로부터 철의 양을 구하고 농도(㎎/ℓ)를 산출한다.On the other hand, the absorbance method that can be easily used in the process test method is based on the following procedure. The pretreated solution was filtered with the first filter paper to dissolve the ferric hydroxide adhering to the filter paper and washed several times with warm water to transfer the filtrate and the washed solution to a 100 ml volumetric flask. Add water to make 70 ml of liquid, add 1 ml of hydroxylamine hydrochloride solution (10 W / V%), and shake. Add 5 ml of o-phenanthroline solution (0.1 W / V%) and shake. Add 10 ml of ammonium acetate solution (50 W / V%), shake and cool to room temperature. Add water to the mark and shake to mix. Leave for 20 minutes to prepare the sample solution. Separately, 50 ml of water is tested according to the test method for the sample, which is used as the blank test solution. Using the blank test solution as a control solution, the absorbance of the sample solution is measured at 510 nm, the amount of iron is determined from the calibration curve prepared in advance, and the concentration (mg / L) is calculated.
상기 방법에서와 같이 흡광광도법이라 할지라도 그 방법이 복잡하고, 철을 수산화철로 전환하여 이를 여과하고 다시 녹이는 과정을 거치게 되어 있어 방법상에서 오류를 유발할 확률이 높아진다. 따라서, 상술한 바와 같은 종래 분석방법의 문제점을 개선시키고 철 농도를 보다 간편하고 신속하게 분석할 수 있는 방법이 요구된다.Even in the absorbance method as in the above method, the method is complicated, and the process of converting iron into iron hydroxide, filtering it, and melting it again increases the probability of causing an error in the method. Therefore, there is a need for a method that can improve the problems of the conventional analytical methods as described above and analyze the iron concentration more simply and quickly.
본 발명의 한 측면은 양액 내의 철 이온 농도를 신속하고 정확하게 확인하는 양액 내의 철 이온 농도 분석방법을 제공하는 것이다.One aspect of the present invention is to provide a method for analyzing the iron ion concentration in the nutrient solution to quickly and accurately identify the iron ion concentration in the nutrient solution.
본 발명의 다른 측면은 양액 내의 철 이온 농도를 신속하고 정확하게 확인할 수 있는 양액 내의 철 이온 농도 분석용 키트를 제공하는 것이다. Another aspect of the present invention is to provide a kit for analyzing the iron ion concentration in the nutrient solution that can quickly and accurately identify the iron ion concentration in the nutrient solution.
본 발명에 의하면, 1,10- 페난트롤린(Phenanthroline) 과 염기(base)가 혼합된 시약에 철 이온이 반응하면 색 변화가 일어난다.According to the present invention, color change occurs when iron ions react with a reagent in which 1,10-phenanthroline and a base are mixed.
a)1,10- 페난트롤린(Phenanthroline) 과 염기(base)를 혼합하여 제조된 제 1시약에 철 이온이 포함된 양액을 첨가하여 색 변화를 확인하는 단계,b) 상기 a)단계의 제 1시약과 동일하게 제조된 표준시약에 농도를 알고 있는 철 이온을 포함하는 표준용액을 각각 가하여 변화된 색을 확인하고,상기 표준시약에 가해진 철 이온의 농도와 상응하여 변화된 색으로부터 표준비색표를 작성한다. 상기 양액의 변화된 색과 작성된 상기 표준비색표를 비교하여, 상기 양액 내의 철 이온 농도를 확인할 수 있다.a) adding a nutrient solution containing iron ions to a first reagent prepared by mixing 1,10-phenanthroline and a base, and confirming the color change, b) the agent of step a) 1) Add a standard solution containing iron ions of known concentration to the standard reagent prepared in the same manner as the reagent, and confirm the changed color, and prepare a standard color table from the changed color corresponding to the concentration of iron ions applied to the standard reagent. do. The concentration of iron ions in the nutrient solution can be confirmed by comparing the changed color of the nutrient solution with the prepared standard colorimetric table.
또한 a) 1,10-페난트롤린(Phenanthroline)과 염기(base)를 혼합하여 제조된 제 1시약에 철 이온이 포함된 양액을 첨가하여 변화된 색을 확인하는 단계; b) 상기 a) 단계의 제1시약과 동일하게 제조된 표준시약에 농도를 알고 있는 철 이온을 포함하는 표준용액을 각각 가하여 흡광도를 측정하고, 상기 표준시약에 가해진 철 이온의 농도와 상응하여 측정된 흡광도로부터 작성된 표준 검량선과 상기 a) 단계의 흡광도를 측정하여 작성된 검량선을 비교하여 상기 양액 내의 철 이온 농도를 확인할 수 있다.In addition, a) adding a nutrient solution containing iron ions to the first reagent prepared by mixing 1,10-phenanthroline (Phenanthroline) and the base (base) to confirm the changed color; b) the absorbance is measured by adding a standard solution containing iron ions of known concentration to the standard reagent prepared in the same manner as the first reagent of step a) and measured in accordance with the concentration of iron ions applied to the standard reagent. The concentration of iron ions in the nutrient solution can be confirmed by comparing the standard calibration curve prepared from the absorbance obtained above with the calibration curve prepared by measuring the absorbance of step a).
본 발명의 다른 견지에 의하면, 1,10-페난트롤린(Phenanthroline)과 탄산수소나트륨(Sodium Bicarbonate)의 중량비를 1:8.5 내지 1:9로 혼합하여 제 1시약을 제조하고, 상기 제 1 시약에 농도를 알고 있는 철 이온 표준용액을 투입하여 미리 작성된 표준검량선에 의해, 제 1시약에 투여한 양액 내의 철 이온 농도를 분석하는 철 이온 농도 분석용 키트를 제공한다.According to another aspect of the present invention, by mixing the weight ratio of 1,10- phenanthroline (Phenanthroline) and sodium bicarbonate (Sodium Bicarbonate) 1: 8.5 to 1: 9 to prepare a first reagent, the first reagent Provided is an iron ion concentration analysis kit for analyzing the iron ion concentration in the nutrient solution administered to the first reagent by injecting the iron ion standard solution having a known concentration into a standard calibration curve.
본 발명의 철 이온 농도 분석방법 및 철 이온 농도 분석용 키트를 이용하여 철 이온 농도를 측정할 경우 기존의 철 이온 농도 측정방법에 비하여 정확할 뿐만 아니라, 철 이온과 시약의 반응 시간이 단축되어 철 이온 농도 검출 시간을 획기적으로 감소시켜 업무 효율을 향상시킬 수 있다. When the iron ion concentration is measured using the iron ion concentration analysis method and the iron ion concentration analysis kit of the present invention, the iron ion concentration is shorter than the conventional iron ion concentration measurement method, and the reaction time between the iron ion and the reagent is shortened. Significantly reduce the concentration detection time can improve work efficiency.
도 1은 본 발명에 따른 철 이온 농도 분석용 키트를 이용하여 철 이온을 포함하는 표준용액을 정량한 검정선을 나타낸 것이다.Figure 1 shows a calibration line quantifying the standard solution containing iron ions using the iron ion concentration analysis kit according to the present invention.
상기의 목적을 달성하기 위하여 본 발명은 1,10- 페난트롤린(Phenanthroline) 과 염기(base)가 혼합된 표준시약에 철 이온이 포함된 표준용액를 첨가후 충분한 시간동안 반응시킨다. In order to achieve the above object, the present invention reacts for a sufficient time after adding a standard solution containing iron ions to a standard reagent mixed with 1,10-phenanthroline and a base.
상기 철 이온은 상기 표준시약과 반응하여 유색의 결합물질(Combined complex)를 형성하게 된다. The iron ions react with the standard reagent to form a colored complex.
이하, 본 발명에 의한 철 이온 농도 분석방법 및 분석용 키트에 대하여 더욱 구체적으로 설명하면 다음과 같다.Hereinafter, the iron ion concentration analysis method and analysis kit according to the present invention will be described in more detail.
본 발명은 양액재배를 위한 양액에 포함된 철의 이온 농도를 측정하기 위한 양액 내의 철 이온 농도 분석방법 및 분석용 키트에 관한 것이다. The present invention relates to an iron ion concentration analysis method and analysis kit in nutrient solution for measuring the ion concentration of iron contained in the nutrient solution for nutrient solution cultivation.
본 발명으로 개선이 이루어지기 전에 이용되던 종래의 철 이온 농도 측정방법은 흡광광도법(페난트로린 법)에 의한 수용액 내 철 이온의 농도 분석 방법으로, 그 방법이 복잡하고, 철을 수산화철로 전환하여 이를 여과하고 다시 녹이는 과정을 거치게 되어 있어 방법상에서 오류를 유발할 확률이 높아진다는 문제가 있었다.Conventional iron ion concentration measurement method used before the improvement is made in the present invention is a method for analyzing the concentration of iron ions in the aqueous solution by the absorbance method (phenanthroline method), the method is complicated, by converting iron to iron hydroxide The process of filtering and re-melting it has the problem of increasing the probability of causing an error in the method.
본 발명에서는, 철을 수산화철로 전환하여 이를 여과하고 다시 녹이는 과정을 거치는 대신에, 1,10- 페난트롤린(Phenanthroline) 과 염기(base)를 혼합하여 제조된 시약과 철 이온을 충분한 시간 동안 반응시켜 일어나는 색 변화를 통해 철 이온 농도를 분석하는바, 신속하고 간편하게 철 이온 농도를 분석할 수 있고, 오류를 유발할 확률이 없다는 장점이 있다.In the present invention, instead of converting iron into iron hydroxide, filtering and re-dissolving it, the reagent prepared by mixing 1,10-phenanthroline and a base and a reaction with iron ions for a sufficient time Analyze the iron ion concentration through the color change that occurs by making a quick, easy analysis of iron ion concentration, there is an advantage that there is no probability of causing an error.
양액 내의 철 이온 농도 분석 방법으로는 a) 1,10-페난트롤린(Phenanthroline)과 염기(base)를 혼합하여 제조된 제 1시약에 철 이온이 포함된 양액을 첨가하여 변화된 색을 확인하는 단계;b) 상기 a) 단계의 제1시약과 동일하게 제조된 표준시약에 농도를 알고 있는 철 이온을 포함하는 표준용액을 각각 가하여 변화된 색을 확인하고, 상기 표준시약에 가해진 철 이온의 농도와 상응하여 변화된 색으로부터 작성된 표준 비색표와 상기 a) 단계의 변화된 색을 비교하여 상기 양액 내의 철 이온 농도를 확인하는 것이 있다.As a method for analyzing the iron ion concentration in the nutrient solution, a) adding a nutrient solution containing iron ions to the first reagent prepared by mixing 1,10-phenanthroline and a base to check the changed color; b) adding a standard solution containing iron ions having a known concentration to a standard reagent prepared in the same manner as the first reagent of step a), and confirming the changed color, and corresponding to the concentration of iron ions applied to the standard reagent. By comparing the standard colorimetric table prepared from the changed color with the changed color of step a) to check the iron ion concentration in the nutrient solution.
또 다른 양액 내의 철 이온 농도 분석 방법으로는 a) 1,10-페난트롤린(Phenanthroline)과 염기(base)를 혼합하여 제조된 제 1시약에 철 이온이 포함된 양액을 첨가하여 변화된 색을 확인하는 단계; b) 상기 a) 단계의 제1시약과 동일하게 제조된 표준시약에 농도를 알고 있는 철 이온을 포함하는 표준용액을 각각 가하여 흡광도를 측정하고, 상기 표준시약에 가해진 철 이온의 농도와 상응하여 측정된 흡광도로부터 작성된 표준 검량선과 상기 a) 단계의 흡광도를 측정하여 작성된 검량선을 비교하여 상기 양액 내의 철 이온 농도를 확인하는 것이 있다.Another method for analyzing the iron ion concentration in the nutrient solution is a) confirming the changed color by adding the iron-containing nutrient solution to the first reagent prepared by mixing 1,10-phenanthroline and base. Making; b) the absorbance is measured by adding a standard solution containing iron ions of known concentration to the standard reagent prepared in the same manner as the first reagent of step a) and measured in accordance with the concentration of iron ions applied to the standard reagent. The standard calibration curve created from the absorbance obtained above is compared with the calibration curve created by measuring the absorbance at step a) to confirm the iron ion concentration in the nutrient solution.
상기 제 1시약은 1,10- 페난트롤린(Phenanthroline)과, 염기(base)의 중량비를 1:7 내지 1:10으로 혼합하여, 바람직하게는 1:8.5 내지 1:9로 혼합하여, 더욱 바람직하게는 1:9로 혼합하여 제조된다.The first reagent is a mixture of 1, 10- phenanthroline (Phenanthroline) and the weight ratio of the base (base) 1: 7 to 1:10, preferably 1: 8.5 to 1: 9, It is preferably prepared by mixing 1: 9.
상기 제1 시약에서 상기 염기(base)는 바람직하게는 탄산수소나트륨(Sodium Bicarbonate)이다.In the first reagent, the base is preferably sodium bicarbonate.
또한 양액 내의 철 이온 농도 분석 방법 이외에도 1,10-페난트롤린(Phenanthroline)과 탄산수소나트륨(Sodium Bicarbonate)의 중량비를 1:8.5 내지 1:9로 혼합하여 제 1시약을 제조하고, 상기 제 1 시약에 농도를 알고 있는 철 이온 표준용액을 투입하여 미리 작성된 표준검량선에 의해, 제 1시약에 투여한 양액 내의 철 이온 농도를 분석하여 양액 내의 철 이온 농도 분석용 키트를 제조할 수 있다.In addition to the method for analyzing the iron ion concentration in the nutrient solution, a first reagent was prepared by mixing a weight ratio of 1,10-phenanthroline and sodium bicarbonate in a ratio of 1: 8.5 to 1: 9. An iron ion standard solution having a known concentration can be added to a reagent, and a standard calibration curve prepared in advance can be used to analyze the iron ion concentration in the nutrient solution administered to the first reagent, thereby preparing a kit for analyzing the iron ion concentration in the nutrient solution.
한편, 분석의 정확성을 기하기 위하여 이미 철 이온 농도를 알고 있는 표준용액을 제조하여 상기 표준시약과 반응시켜 흡광도를 측정한 결과, 다음 도 1에 나타낸 바와 같은 결과를 얻을 수 있었다. On the other hand, in order to ensure the accuracy of the analysis prepared a standard solution that already knows the iron ion concentration and reacted with the standard reagent to measure the absorbance, the results as shown in Figure 1 was obtained.
또한 수 회에 걸친 실험에서, 본 발명에 의한 양액 내의 철 이온 농도를 측정하기 위한 분석용 키트를 이용할 때에 양액 중에 포함된 다른 종류의 이온에 의한 간섭(interfere) 영향을 배제할 수 있는 한계치를 조사한 결과 [표 1]과 같은 결과를 얻을 수 있었다.In addition, in several experiments, when using an analytical kit for measuring the iron ion concentration in the nutrient solution according to the present invention, the limit value that can exclude the influence of interference by other kinds of ions contained in the nutrient solution was investigated. Results The results as shown in [Table 1] were obtained.
10 mg/l
10 mg / l
상기의 조사 결과를 통하여 양액 중에 포함된 다양한 종류의 이온에 대하여 일정한 범위에서 간섭을 배제할 수 있음을 알 수 있으며, 여러 종류의 이온이 포함된 시료에 대하여도 적용할 수 있는 방법임을 알 수 있다.Through the above research results, it can be seen that interference can be excluded in a certain range with respect to various kinds of ions contained in the nutrient solution, and it can be seen that the method can be applied to samples containing various kinds of ions. .
흡광도를 즉시 측정할 수 있는 직경 1 인치(inch)의 원형의 유리 시험관을 준비하고, 여러 개의 시험관에 1,10- 페난트롤린(Phenanthroline)과 탄산수소나트륨(Sodium Bicarbonate)의 중량비를 1:9로 제조된 시약 분말 500mg을 분취한다. 이렇게 준비된 시험관에 철 이온 표준액으로서 철 이온 농도 0~0.25mg/L를 준비하고, 상기 표준액 2mL를 취하여 시험관에 투입한다. 2분동안 반응시킨 후 510nm의 측정파장으로 흡광도를 측정하여 검량선을 작성하였다. 상기 결과는 도 1에 나타내었으며,상기 실험은 각각 10회씩 반복하였다.이 때 검량선은 시료를 500mg 을 사용했을 경우 철 이온 농도 0.25ml/L까지 우수한 선형의 상관관계를 보임을 알 수 있다.Prepare a round glass test tube with a diameter of 1 inch that can measure the absorbance immediately, and the weight ratio of 1,10- phenanthroline and sodium bicarbonate is 1: 9 in several test tubes. Aliquot 500 mg of the prepared reagent powder. Thus prepared iron ion concentration 0-0.25 mg / L as an iron ion standard solution to the test tube prepared, 2mL of the standard solution is added to the test tube. After reacting for 2 minutes, the absorbance was measured using a measurement wavelength of 510 nm to prepare a calibration curve. The results are shown in Figure 1, the experiment was repeated 10 times each. At this time the calibration curve can be seen that the excellent linear correlation to the iron ion concentration of 0.25ml / L when using the sample 500mg.
Claims (6)
b) 상기 a) 단계의 제1시약과 동일하게 제조된 표준시약에 농도를 알고 있는 철 이온을 포함하는 표준용액을 각각 가하여 변화된 색을 확인하고, 상기 표준시약에 가해진 철 이온의 농도와 상응하여 변화된 색으로부터 작성된 표준 비색표와 상기 a) 단계의 변화된 색을 비교하여 상기 양액 내의 철 이온 농도를 확인하는 것을 특징으로 하는 양액 내의 철 이온 농도 분석 방법.a) confirming the changed color by adding a nutrient solution containing iron ions to the first reagent prepared by mixing 1,10-phenanthroline and a base;
b) adding a standard solution containing iron ions of known concentration to the standard reagent prepared in the same manner as the first reagent of step a), and confirming the changed color, and corresponding to the concentration of iron ions applied to the standard reagent. A method for analyzing the iron ion concentration in nutrient solution, characterized in that the iron color concentration in the nutrient solution is confirmed by comparing the standard colorimetric table prepared from the changed color with the changed color of step a).
b) 상기 a) 단계의 제1시약과 동일하게 제조된 표준시약에 농도를 알고 있는 철 이온을 포함하는 표준용액을 각각 가하여 흡광도를 측정하고, 상기 표준시약에 가해진 철 이온의 농도와 상응하여 측정된 흡광도로부터 작성된 표준 검량선과 상기 a) 단계의 흡광도를 측정하여 작성된 검량선을 비교하여 상기 양액 내의 철 이온 농도를 확인하는 것을 특징으로 하는 양액 내의 철 이온 농도 분석 방법.
a) adding a nutrient solution containing iron ions to the first reagent prepared by mixing 1,10-phenanthroline and base in a weight ratio of 1: 8.5 to 1: 9 to confirm the changed color; step;
b) the absorbance is measured by adding a standard solution containing iron ions of known concentration to the standard reagent prepared in the same manner as the first reagent of step a) and measured in accordance with the concentration of iron ions applied to the standard reagent. A method for analyzing the iron ion concentration in the nutrient solution, characterized in that the iron ion concentration in the nutrient solution is confirmed by comparing the standard calibration curve prepared from the absorbance measured with the absorbance of the step a).
The method of claim 1 or 2, wherein the base is sodium bicarbonate.
The method of claim 1, wherein the weight ratio of the 1,10-phenanthroline (Phenanthroline) and the base (base) is 1: 8.5 to 1: 9.
A first reagent was prepared by mixing a weight ratio of 1,10-phenanthroline and sodium bicarbonate in a ratio of 1: 8.5 to 1: 9, and an iron ion standard having a known concentration in the first reagent. Kit for analyzing the iron ion concentration in the nutrient solution for analyzing the iron ion concentration in the nutrient solution administered to the first reagent by the standard calibration curve prepared by adding the solution
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| KR102234297B1 (en) * | 2019-10-16 | 2021-03-31 | 대한민국 | Reagent composition for detection of ion composition in hydroponic nutrients and detection kit using thereof |
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