KR100708984B1 - Effect of sprout blended powderbroccoli sprout, alfalfa sprout, red clover sprout on heavy metal detoxification and the Saengshik composition containing theses powder - Google Patents

Abstract

The present invention relates to a reproductive product composition for reducing heavy metal toxins, and more specifically, to a general reproductive composition containing a mixed composition of broccoli sprouts, alfalfa sprouts, and red clova sprouts in which heavy metal detoxifying effects have been confirmed, and thus detoxifying heavy metals. It relates to a fortified product composition.

Raw Food, Composition, Broccoli Sprout, Alfalfa Sprout, Red Clover Sprout, Sprout Vegetable, Heavy Metal, Cadmium, Lead

Description

Effect of sprout blended powder (broccoli sprout, alfalfa sprout, red clover sprout) on heavy metal detoxification and the Saengshik with a heavy metal detoxification effect (broccoli sprout, alfalfa sprout, red clover sprout) composition containing theses powder}

Figure 1. Changes in body weight of laboratory rats during the test period

Figure 2. Changes in body weight of rats during diet with heavy metals

  (A) Body weight change in laboratory rats during lead and dietary supply

  (B) Body weight change of laboratory rats during cadmium and dietary supply

The present invention relates to a reproductive health food, and more particularly, by adding broccoli sprouts, alfalfa sprouts, and red clova sprouts, which have high nutritional density and heavy metal detoxification effects, to raw foods containing vegetable raw materials, to protect the human body from heavy metals. It relates to a composition of raw products that can help to.

The rapid development of industrial society has made our lives convenient, but our lives are threatened directly or indirectly due to environmental pollution. According to data from the World Health Organization (WHO), more than 3 million people die every year from respiratory diseases and heart diseases caused by air pollution from vehicles and factories. According to a study on 'Estimate the social cost of pollution', a study revealed that air pollution is likely to cause 11,000 deaths early and economic losses of up to 10 trillion won in the Seoul metropolitan area. In particular, due to the development of the industry, the pollution of harmful heavy metals such as lead and cadmium has increased rapidly, and the pollution caused by it is serious and it is becoming a big social problem at home and abroad.

When these harmful substances are not metabolized or excreted, cellular metabolism is broken and toxic. Homeostasis refers to the balance of function in the body. When the amount of harmful substances is greater than the amount that can be handled by the body, the balance is broken. Therefore, through toxins and detoxification process for daily health, toxins and wastes can be cleaned and purified to improve basic function.

When harmful substances are introduced into the body, biological transformation (biotransformation) action to quickly excrete the harmful substances in the body proceeds, and eventually through this action the harmful substances are excreted in vitro. This metabolic process is explained in two main steps. That is, foreign substances such as environmental pollutants or drugs absorbed into the body are excreted in vitro by the phasel or phase2 reactions in the liver tissue or through both stages. Phasel enzymes include cytochrom P450, which converts precancerous sources into highly reactive compounds by oxidation, reduction and hydrolysis of hydrophobic compounds to have reactive electrons. Phase2 enzymes include glutathione S-transferase, UDP-glucuronosyl transferase, sulfotransferase, and guinone reductase, which convert highly metabolites into stable substances to facilitate excretion (Liska DJ. Alter Med Rev 3: 187-198, 1998). .

Epidemiologic studies have reported negative correlations with risk factors for lung cancer, stomach cancer, colorectal cancer, rectal cancer and prostate cancer in groups with high consumption of cruciferous vegetables. Cruciferous vegetables include broccoli, cauliflower, cabbage, and kale, and their anticancer activity has been found to be due to glucosinolates, a sulfur-containing substance. As a result, many studies have been conducted on the relationship between glucosinolates and cancer, and studies have shown that glucosinolates have anticancer activity by changing the expression of biotransformation enzymes (Dorette TH et al., Chem Biol Interact 103: 79-129). , 1997). It is known that cruciferous plants, especially broccoli sprouts, contain 10 to 100 times higher content of isothiocyanate containing sulforaphane than common broccoli, and this substance is known to induce phase 2 enzyme activity (Fahey JW et al., Proc Natl Acad Sci 94: 11149-11151, 1997).

Reproductive food is a concept opposite to flowering, and is currently a commercially available processed food for breakfast as a category of health-oriented food. Most reproductive products in Korea are dried and mixed with various grains, seeds, vegetables, fruits, and seaweeds by low temperature drying method. Since there is no heat treatment process, there are no nutrients such as dietary fiber, vitamins and minerals. It is rich in physiologically active substances that are spotlighted as plant compounds. Therefore, the enzymes, minerals, and vitamins necessary for human metabolism are preserved to actively promote metabolism, eliminating various wastes, normalizing bowel movements and relieving constipation, suppressing the increase of cholesterol in the blood, weight loss effect due to low calorie intake, and regular meals. It is promoted as a food that helps form habits. In addition, it can be consumed without destroying chlorophyll as a non-heated food. Chlorophyll has a blood-like structure and is thought to prevent diseases by purifying the human body by acting hematopoiesis and detoxification. In fact, research shows that people who reproduce have lower rates of various diseases than people who have a fire.

However, studies using reproductive products have shown the effects on antioxidant system and serum minerals (Park J et al., Kor J Nutr 36: 197-206, 2003), the effects on obesity and blood components of overweight obese women (Park SH et al., J East Asian Soc.Dietary Life 13: 39-55, 2003), improving fatty liver (Lee E et al., J Korean Soc Food Sci Nutr 32: 256-262, 2003), and health improvement effects on hyperlipidemia ( Yang BK et al., Kor J life science 11: 298-303, 2001), and anti-diabetic effects (Kim M et al., J Med Food 7: 162-167, 2004). There is no scientific data on raw food products, which have a problem that low raw intake of the compound that shows efficacy as raw food raw materials, rather than extracts of certain ingredients.

The present invention has been devised in view of the above point, the object of the present invention is a reproductive product composition for reducing toxins, which shows a protective effect against harmful heavy metals lead and cadmium toxicity, more specifically, high nutrient density and detoxification to cadmium Adding broccoli sprouts, alfalfa sprouts, and red clova sprouts to the reproductive products to prevent anemia, which is a sign of heavy metal toxicity, prevent damage to liver and oxidative stress, and inhibit the accumulation of heavy metals in the body. To constitute a reproductive product composition that can reduce the damage caused by harmful heavy metals lead and cadmium.

To achieve this purpose, the reproductive product composition for reducing toxins, which may exhibit a protective effect on lead and cadmium toxicity according to the present invention, has a high nutritional density and excellent detoxification activity in broccoli sprouts 40-70% by weight, Alfalfa sprouts 10-50% by weight, red Clover buds 1-30% by weight of the sprouted vegetable mixed composition containing 0.5% by weight or more to be effectively used.

Hereinafter, the present invention will be described in detail.

Shooting is the first process of inactivating DNA genetic information, various enzymes, and nutrients in seeds and embryos of seeds to be activated and start life as plants when external environmental conditions improve. Therefore, sprouted vegetables have the advantage of high nutritional density due to the production of large amounts of vitamins and minerals, activated enzymes and functional compounds. The sprouted vegetable mixed composition used in the present invention is a germinated broccoli (Fahey JW et al., Proc) containing 10 to 100 times more isothiocyanates compounds including sulfolafan, which induce detoxification enzymes, together with the above-mentioned properties. Natl Acad Sci 94: 11149-11151, 1997), which is preferably used in the present composition.

Therefore, broccoli sprouts, alfalfa sprouts, and red clova sprouts, which are the main raw materials having a cadmium detoxifying effect, which are harmful heavy metals according to the present invention, are germinated at 20 ± 5 ° C. for 1-7 days by soaking each plant seed. Is 0.1cm or more and 6cm or less, and 40-70% by weight of broccoli sprouts, 10-50% by weight of alfalfa sprouts, and 1-25% by weight of red clova sprouts.

In addition, in order to apply the sprouted vegetable mixed composition to a raw product, a general reproductive composition was composed and tested for heavy metal detoxification at a four-level concentration. As a result, the sprouted vegetable mixed composition contained 0.5 wt% or more of lead and cadmium. A toxin alleviation reproductive product composition exhibiting a protective effect against toxicity was constructed.

The reason why the sprouted vegetable mixture composition has a heavy metal detoxifying effect even at such low concentrations is that the sprouted vegetable raw material itself has a high nutritional density as described above, and may also be attributed to the synergistic effect of interaction with the reproductive composition. Hereinafter, the present invention will be described in detail.

delete

Example 1

62.5% by weight of broccoli sprouts, 25% by weight of alfalfa sprouts, and 12.5% by weight of red clova sprouts are mixed in each drying ratio to make a sprouted vegetable mixture composition. 20 weight%, 20 weight% of pumpkin, 10 weight% of bellflower, 10 weight% of kale, 10 weight% of mugwort, and 10 weight% of pine needles were mixed, and the vegetable mixed composition was manufactured.

Test Example 1

Example 1 In order to determine the detoxification effect on the cadmium of the composition was carried out as follows.

Stage 1: Animal Breeding and Experimental Diet

40 male rats (Spraque Dawley) of 5 weeks of age were purchased (Samtako BIOKOREA, Gyeonggi-do) and then adapted to general solid feed for 1 week (temperature 23 ± 3 ℃, relative humidity 55 ± 15%, Contrast cycle 12 hours), and the control group administered with cadmium after the cadmium-treated negative control group and the cadmium-treated group as shown in Table 1 according to the randomized complete block design, and then again the cadmium-treated group according to the composition of the experimental diet The experimental diet was divided into 10 groups, each of which was divided into a control group (composition-free group), a test group (addition of sprouted vegetable mixed composition), and a comparative group (group mixed with vegetable mixed composition), and the experimental diet was supplied for 4 weeks. All experimental diets were freely fed as a powdered diet, and the basic diet was AIN-76A diet composition (casein, corn starch, corn oil, sugar, cellulose, AIN-76A vitamin mixture ^{Note 1)} , AIN-76A mineral mixture ^{Note 2)} , choline chloride, methionine). Cadmium was supplied as cadmium chloride at 350ppm of dietary weight, and the sprouted vegetable and vegetable blends were formulated to contain 10% of the feed weight of each feed. Was made the same. That is, the amount of protein contained in the composition was corrected so that the ratio of carbohydrate: protein: fat was constant by subtracting the amount of fat from casein from corn oil.

TABLE 1

delete

delete

As a result of measuring the body weight of the experimental animals every week during the 4-week diet, the weight increase rate was in the order of negative control group> test group> comparison group> positive control group. The increase slowed down and showed heavy metal toxicity symptoms (FIG. 1).

Stage 2: Sampling and Blood Biochemical Analysis

After the end of the experiment, the animals were fasted for 12 hours, anesthetized with ether, and blood and liver tissues were collected. Blood was centrifuged at 1000 x g for 15 minutes and used for serum analysis. After extraction, the liver was washed with physiological saline and stored at -80 ° C for heavy metal analysis. The separated serum was analyzed using GIO (gluamic oxaloacetic transaminase), GPT (gluamic pyruvic transaminase), and urea nitrogen (BUN: indicator of renal function) using a blood biochemical analyzer (AU400, Olympus). blood urea nitrogen) and creatinine were measured and the results are shown in Table 2.

TABLE 2

As shown in Table 2, the positive control group showed significant increase in gephyti, geoty, and urea nitrogen levels compared to the negative control group to confirm the damage caused by cadmium. Gephyti and geotyse enzyme activity was significantly lower (p <0.05) compared to the positive control, and urea nitrogen, a marker of kidney damage, was also significantly decreased (p <0.05) compared to the positive control. However, the comparison group could not confirm the effect in any item compared to the positive control group. According to the above results, the sprouted vegetable mixed composition was found to restore liver and kidney damage damaged by cadmium, and the vegetable mixed composition did not show this effect.

Step 3: Determination of Heavy Metal Content

Cadmium content in liver tissue is taken in a certain amount of the sample taken in step 2, dry decomposition for 24 hours in 550 ℃ incinerator, dissolved with concentrated nitric acid, diluted with 1N hydrochloric acid and the atomic absorbance (AAs, Cambridge) at wavelength 228.8nm , UK).

TABLE 3

As shown in Table 3, it was confirmed that cadmium accumulated in the liver organs by the administration of cadmium, and the test group showed that the content dropped to 64.4% of the positive control group, and the comparative group showed a similar tendency as the positive control group. Indicated. Therefore, the sprouted vegetable mixture composition appeared to inhibit the accumulation of heavy metals in the liver, and the vegetable mixture composition did not appear to inhibit the accumulation of heavy metals.

Example 2

Brown rice 47.5%, brown rice glutinous rice 6.2%, brown soybean 11%, germinated brown rice 8.6%, barley 8.7%, seed 5.4%, brown rice slice 1%, barley 0.5%, kale 0.28%, light green 0.28 Weight%, Broccoli 0.12%, Spinach 0.64%, Carrot 0.52%, Cabbage 0.4%, Onion 0.1%, Potato 0.9%, Sweet Potato 0.4%, Kelp 0.06%, Seaweed 0.06%, Seaweed 0.15 A raw composition was prepared (wt%, 0.08 wt%, mugwort 0.31 wt%, zucchini 0.2 wt%, dietary fiber 0.6 wt%, fructose 4 wt% (content 100%), and the sprouted vegetable mixed composition of Example 1 was tested. Four sections of reproductive products were prepared by mixing with the reproductive composition configured above in a 0.25% to 5% by weight ratio.

Test Example 2

Based on the results carried out in Test Example 1, a raw product composition composed of a sprouted vegetable mixed composition identified as an effective ingredient as in Example 2 was used to confirm the protective effect against cadmium and lead toxicity. Was carried out.

Stage 1: Animal Breeding and Experimental Diet

110 male rats, 5 weeks of age, were purchased (Samtako BIOKOREA, Gyeonggi-do) and then adapted to a solid feed for a week (temperature 23 ± 3 ℃, relative humidity 55 ± 15%, contrast cycle 12 hours). Negative control group (n = 10), cadmium group (n = 50) and lead group (n = 50) as shown in Table 4 by randomized complete block design Each group of heavy metals was divided into 5 groups of 10 animals according to the concentration of the sprouted vegetable mixed composition contained in the raw product composition (positive control group-sprouted vegetable mixed composition-free group, test group 1-0.25 wt% Vegetable mixed composition administration group, test group 2-0.5% by weight of sprouted vegetable mixed composition, test group 3-1% by weight sprouted vegetable mixed composition, test group 4-5% by weight of sprouted vegetable mixed composition) Experimental diet was supplied for 6 weeks. It was. All experimental diets were freely fed as a powdered diet, and the general diet consisted of AIN-76A diet composition (casein, corn starch, corn oil, sugar, cellulose, AIN-76A vitamin mixtures ⁾ , and AIN-76A mineral mixtures ^{. 2)} , choline chloride, methionine), and the nutrient density of each group was adjusted to equal the amount of other nutrients consumed per calorie. Lead acetate was administered using lead acetate ([Pb (C ₂ H ₃ O ₂ ) ₃ H ₂ O). It was not dissolved in negative water and was orally administered at a concentration of 12 mg / rat once daily for 6 weeks, and cadmium chloride (cadmium chloride) CdCl ₂ 1 / 2H ₂ O) was used orally once daily for 6 weeks at a concentration of 1.8 mg / rat.

TABLE 4

delete

The weight gain rate was measured in the order of negative control group> test group 4> test group 3> test group 2> test group 1> positive control group for 6 weeks. The negative control group showed normal growth and positive control group. Silver was affected by heavy metals, the weight growth rate tended to slow down (Fig. 2).

Stage 2: Sampling and Blood Biochemical Analysis

After the experiment, the animals were fasted for 12 hours, anesthetized with ether, and blood, liver and kidney tissues were collected. Whole blood was used for hemocytosis, and some were centrifuged at 1000 x g for 15 minutes and used for serum analysis. Liver and kidney were washed with physiological saline after extraction and stored at -80 ℃. Blood cell analysis measures hemoglobin (HGB), hematocrit (HCT), mean erythrocyte volume (MCV), mean erythrocyte hemoglobin (MCH), and red blood cell volume distribution (RDW) using an automated hemocytometer (Cell dyn 3700, Abbot, USA) The serum was measured using a blood biochemical analyzer (AU400, Olympus) to measure liver function test indicators such as geoty, gephyti and renal function test elements urea nitrogen, creatinine.

TABLE 5

As shown in Table 5, hemoglobin, hematocrit, mean hemoglobin volume, mean hemoglobin, and red blood cell total distribution showed significant numerical changes compared to negative control when heavy metals were administered. When the vegetable mixture composition was administered, the value was observed to be recovered in a concentration-dependent manner, and the ingestion of the raw product composition containing the sprouted vegetable mixture composition appeared to reduce the damage caused by heavy metals. In particular, the effect on lead is in the section containing 0.5% by weight or more of the sprouted vegetable mixed composition, and the effect by cadmium is effective in the section containing 0.25% or more by weight of the sprouted vegetable mixed composition. The raw product composition can be seen to reduce the hematologic toxicity symptoms caused by lead and cadmium.

TABLE 6

Table 6 shows the results of measuring the major enzyme activity in serum to determine the degree of damage to liver and kidney tissue by heavy metals. In the case of Zippy enzyme activity, which is an indicator of liver damage, the activity was significantly increased when cadmium was administered, and the group added more than 0.5% by weight of sprouted vegetable mixed composition showed significantly lower activity than the positive control group, indicating that liver damage caused by cadmium was suppressed. Appeared. However, other indicators did not show any damage by heavy metals, and therefore, the effects of the sprouted vegetable mixture composition could not be determined.

According to the above results, the raw product composition containing 0.5 wt% or more of the sprouted vegetable mixed composition can be seen to prevent liver damage caused by cadmium.

Step 3: Determination of Antioxidant Capacity in the Liver

After rinsing the liver tissue collected in step 2 with physiological saline, using a buffer solution, the ratio of liver and solution was mixed to 1: 9, homogenized and centrifuged at 4 ℃, 3,000 xg for 10 minutes to take a supernatant. This was used to measure the superoxide dismutase (SOD) enzyme activity, lipid peroxidation and glutathione content. SOD activity was measured using SOD Assay kit (Calibiochem ^® , CA, USA). Lipid peroxidation and glutathione concentrations were measured using Lipid peroxidation assay kit (Calibiochem ^® , CA, USA) and Biooxytech ^® GSH-400TM (OXISResarch ^TM). , OR, USA).

TABLE 7

Antioxidant efficacy in liver is shown in Table 7. Lead and cadmium administration significantly altered lipid peroxidation and glutathione contents compared to the negative control group (p <0.05), and ingestion of sprouted vegetable blend composition was restored to normal level. It can be inferred that he reduced the In the case of S-Odi activity, no change was observed according to heavy metal administration, but the sprouted vegetable mixture composition was found to enhance the S-ODI activity.

As a result, lipid peroxidation and glutathione showed oxidative damage due to lead and cadmium, and the result was effective in the section containing 0.25% by weight or more of sprouted vegetable mixed composition, and ultimately the raw product composition containing 0.25% or more by weight of sprouted vegetable mixed composition It can be seen that it can prevent oxidative damage by heavy metal.

Fourth Step: Determination of Heavy Metal Content

The content of cadmium and lead in the liver tissues is determined by taking a certain amount of the sample taken in step 2, dry decomposition for 24 hours in a 550 ℃ incinerator, dissolved with concentrated nitric acid, diluted with 1N hydrochloric acid, and an atomic absorbance (AAs) at wavelength 228.8nm. Cambridge, UK).

TABLE 8

The heavy metal content in liver and kidney tissue is shown in Table 8. That is, the lead concentration in the liver was significantly reduced (p <0.05) in the reproductive composition group containing more than 25% by weight of the mixed vegetable composition, and the lead concentration in the kidney was 0.5 wt%. % And 5% by weight of the mixed vegetable composition was significantly decreased (p <0.05). Cadmium accumulation in the liver tended to be slightly lower than the group that induced cadmium poisoning in the section containing more than 25% by weight of sprouted vegetable mixed composition. The concentration was significantly decreased (p <0.01).

Therefore, raw food composition containing 0.5% by weight or more of sprouted vegetable mixed composition seems to be able to suppress the accumulation of heavy metals.

According to the present invention, the sprouted vegetable mixed composition has a heavy metal detoxifying effect, and the raw product composition containing 0.5% by weight or more of the sprouted vegetable mixed composition may be provided as a composition that is beneficial for reproductive product application as a protection against lead and cadmium toxicity. have.

Claims (2)

A sprouted vegetable mixed composition containing 40-70% by weight of broccoli sprouts, 10-50% by weight of alfalfa sprouts, and 1-30% by weight of red clova sprout. Raw material composition for reducing heavy metal toxins, characterized in that it contains 0.5 wt% or more of the sprouted vegetable mixed composition of claim 1 in a dry content.

Images