JPWO2023013498A1 - - Google Patents

Info

Publication number
JPWO2023013498A1
JPWO2023013498A1 JP2023540286A JP2023540286A JPWO2023013498A1 JP WO2023013498 A1 JPWO2023013498 A1 JP WO2023013498A1 JP 2023540286 A JP2023540286 A JP 2023540286A JP 2023540286 A JP2023540286 A JP 2023540286A JP WO2023013498 A1 JPWO2023013498 A1 JP WO2023013498A1
Authority
JP
Japan
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023540286A
Other languages
Japanese (ja)
Other versions
JPWO2023013498A5 (https=
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of JPWO2023013498A1 publication Critical patent/JPWO2023013498A1/ja
Publication of JPWO2023013498A5 publication Critical patent/JPWO2023013498A5/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/02Oxidoreductases acting on the CH-OH group of donors (1.1) with a cytochrome as acceptor (1.1.2)
    • C12Y101/02003L-Lactate dehydrogenase (cytochrome) (1.1.2.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027L-Lactate dehydrogenase (1.1.1.27)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2023540286A 2021-08-03 2022-07-27 Pending JPWO2023013498A1 (https=)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021127638 2021-08-03
PCT/JP2022/028959 WO2023013498A1 (ja) 2021-08-03 2022-07-27 新規フラビン依存性乳酸デヒドロゲナーゼ及び乳酸デヒドロゲナーゼの安定性を向上させる方法

Publications (2)

Publication Number Publication Date
JPWO2023013498A1 true JPWO2023013498A1 (https=) 2023-02-09
JPWO2023013498A5 JPWO2023013498A5 (https=) 2025-09-09

Family

ID=85154538

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023540286A Pending JPWO2023013498A1 (https=) 2021-08-03 2022-07-27

Country Status (4)

Country Link
US (1) US20240401002A1 (https=)
EP (1) EP4382608A4 (https=)
JP (1) JPWO2023013498A1 (https=)
WO (1) WO2023013498A1 (https=)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4884733B2 (ja) * 2005-09-15 2012-02-29 オリエンタル酵母工業株式会社 D−乳酸脱水素酵素
WO2016205378A1 (en) 2015-06-15 2016-12-22 Abbott Diabetes Care Inc. Stabilized lactate responsive enzymes, electrodes and sensors, and methods for making and using the same
CN109906270A (zh) * 2016-09-16 2019-06-18 嘉吉公司 经遗传修饰的乳酸消耗酵母以及使用此类经遗传修饰的酵母的发酵工艺
JP2021078427A (ja) * 2019-11-19 2021-05-27 アイシン精機株式会社 D−乳酸脱水素酵素変異体、d−乳酸脱水素酵素変異体を備えたバイオ燃料電池、及び、バイオセンサ
US20230213499A1 (en) * 2020-02-21 2023-07-06 Kikkoman Corporation Device for evaluating state of sample, system containing same, method for evaluating state of sample and lactate dehydrogenase used therefor
CN112813043A (zh) * 2021-02-23 2021-05-18 江南大学 一种酶活及稳定性提高的l-乳酸脱氢酶突变体及其应用

Also Published As

Publication number Publication date
EP4382608A1 (en) 2024-06-12
US20240401002A1 (en) 2024-12-05
WO2023013498A1 (ja) 2023-02-09
EP4382608A4 (en) 2025-07-16

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Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250722

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250901