JPWO2022198080A5 - - Google Patents
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- Publication number
- JPWO2022198080A5 JPWO2022198080A5 JP2023556950A JP2023556950A JPWO2022198080A5 JP WO2022198080 A5 JPWO2022198080 A5 JP WO2022198080A5 JP 2023556950 A JP2023556950 A JP 2023556950A JP 2023556950 A JP2023556950 A JP 2023556950A JP WO2022198080 A5 JPWO2022198080 A5 JP WO2022198080A5
- Authority
- JP
- Japan
- Prior art keywords
- ribonucleic acid
- sequence
- engineered guide
- class
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920002477 rna polymer Polymers 0.000 claims 30
- 108010042407 Endonucleases Proteins 0.000 claims 21
- 102000004533 Endonucleases Human genes 0.000 claims 21
- 108020004707 nucleic acids Proteins 0.000 claims 15
- 102000039446 nucleic acids Human genes 0.000 claims 15
- 150000007523 nucleic acids Chemical class 0.000 claims 15
- 238000000034 method Methods 0.000 claims 14
- 108091008874 T cell receptors Proteins 0.000 claims 6
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims 6
- 125000006850 spacer group Chemical group 0.000 claims 6
- 102100033417 Glucocorticoid receptor Human genes 0.000 claims 5
- 210000001744 T-lymphocyte Anatomy 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 5
- 101000926939 Homo sapiens Glucocorticoid receptor Proteins 0.000 claims 3
- 239000003862 glucocorticoid Substances 0.000 claims 3
- 102000009027 Albumins Human genes 0.000 claims 2
- 108010088751 Albumins Proteins 0.000 claims 2
- 101710124882 Glucocorticoid receptor Proteins 0.000 claims 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 2
- 230000009615 deamination Effects 0.000 claims 2
- 238000006481 deamination reaction Methods 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 239000002243 precursor Substances 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 108700004991 Cas12a Proteins 0.000 claims 1
- 108020004705 Codon Proteins 0.000 claims 1
- 108020004485 Nonsense Codon Proteins 0.000 claims 1
- 229960000643 adenine Drugs 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 229940104302 cytosine Drugs 0.000 claims 1
- 231100000221 frame shift mutation induction Toxicity 0.000 claims 1
- 230000037433 frameshift Effects 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163163510P | 2021-03-19 | 2021-03-19 | |
| US63/163,510 | 2021-03-19 | ||
| US202163186506P | 2021-05-10 | 2021-05-10 | |
| US63/186,506 | 2021-05-10 | ||
| US202163241916P | 2021-09-08 | 2021-09-08 | |
| US63/241,916 | 2021-09-08 | ||
| PCT/US2022/021004 WO2022198080A1 (en) | 2021-03-19 | 2022-03-18 | Multiplex editing with cas enzymes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2024515936A JP2024515936A (ja) | 2024-04-11 |
| JPWO2022198080A5 true JPWO2022198080A5 (https=) | 2025-03-25 |
| JP2024515936A5 JP2024515936A5 (https=) | 2025-03-25 |
Family
ID=83321225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023556950A Pending JP2024515936A (ja) | 2021-03-19 | 2022-03-18 | Ｃａｓ酵素を用いたマルチプレックス編集 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20250075233A1 (https=) |
| EP (1) | EP4308699A4 (https=) |
| JP (1) | JP2024515936A (https=) |
| KR (1) | KR20230157387A (https=) |
| AU (1) | AU2022237663A1 (https=) |
| BR (1) | BR112023018948A2 (https=) |
| CA (1) | CA3210361A1 (https=) |
| MX (1) | MX2023010969A (https=) |
| WO (1) | WO2022198080A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2617658B (en) | 2020-03-06 | 2024-04-17 | Metagenomi Inc | Class II, type V CRISPR systems |
| KR20230021657A (ko) * | 2020-05-08 | 2023-02-14 | 메타지노미, 인크. | Ruvc 도메인을 포함하는 효소 |
| BR112023014719A2 (pt) * | 2021-01-22 | 2023-12-05 | Metagenomi Inc | Novas nucleases quiméricas e manipuladas |
| CA3225082A1 (en) | 2021-08-27 | 2023-03-02 | Brian C. Thomas | Enzymes with ruvc domains |
| MX2024006257A (es) | 2021-11-24 | 2024-07-29 | Metagenomi Inc | Sistemas de endonucleasas. |
| CA3277009A1 (en) * | 2022-12-16 | 2024-06-20 | Life Edit Therapeutics, Inc. | RNA GUIDELINES TARGETING THE TRAC GENE AND METHODS OF USE |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099215A2 (en) * | 2002-05-20 | 2003-12-04 | Pharmacia Corporation | Antisense modulation of glucocorticoid receptor expression |
| US20150017136A1 (en) * | 2013-07-15 | 2015-01-15 | Cellectis | Methods for engineering allogeneic and highly active t cell for immunotherapy |
| US20180119174A1 (en) * | 2015-05-13 | 2018-05-03 | Seattle Children's Hospita (dba Seattle Children's Research Institute | Enhancing endonuclease based gene editing in primary cells |
| KR102438360B1 (ko) * | 2016-03-04 | 2022-08-31 | 에디타스 메디신, 인코포레이티드 | 암 면역요법을 위한 crispr-cpf1-관련 방법, 조성물 및 구성성분 |
| MX2019013514A (es) * | 2017-05-12 | 2020-01-20 | Crispr Therapeutics Ag | Materiales y metodos para modificar celulas por ingenieria genetica y usos de los mismos en inmunooncologia. |
| TW201920661A (zh) * | 2017-09-18 | 2019-06-01 | 大陸商博雅輯因（北京）生物科技有限公司 | 一種基因編輯t細胞及其用途 |
| AU2019214935B2 (en) * | 2018-01-30 | 2025-06-26 | Editas Medicine, Inc. | Systems and methods for modulating chromosomal rearrangements |
| US12275951B2 (en) * | 2018-02-06 | 2025-04-15 | Ohio State Innovation Foundation | Engineered guide RNA and uses thereof |
| CA3089587A1 (en) * | 2018-02-08 | 2019-08-15 | Sangamo Therapeutics, Inc. | Engineered target specific nucleases |
| US10913941B2 (en) * | 2019-02-14 | 2021-02-09 | Metagenomi Ip Technologies, Llc | Enzymes with RuvC domains |
| AU2020223370B2 (en) * | 2019-02-14 | 2023-04-20 | Metagenomi Therapeutics, Inc. | Enzymes with RuvC domains |
| US20220145333A1 (en) * | 2019-03-11 | 2022-05-12 | Sorrento Therapeutics, Inc. | Improved process for integration of dna constructs using rna-guided endonucleases |
| GB201907733D0 (en) * | 2019-05-31 | 2019-07-17 | Governing Council Of The Univ Of Toronto | Methods and compositions for multiplex gene editing |
-
2022
- 2022-03-18 BR BR112023018948A patent/BR112023018948A2/pt active Search and Examination
- 2022-03-18 EP EP22772310.3A patent/EP4308699A4/en active Pending
- 2022-03-18 JP JP2023556950A patent/JP2024515936A/ja active Pending
- 2022-03-18 MX MX2023010969A patent/MX2023010969A/es unknown
- 2022-03-18 CA CA3210361A patent/CA3210361A1/en active Pending
- 2022-03-18 WO PCT/US2022/021004 patent/WO2022198080A1/en not_active Ceased
- 2022-03-18 AU AU2022237663A patent/AU2022237663A1/en active Pending
- 2022-03-18 KR KR1020237034371A patent/KR20230157387A/ko active Pending
-
2023
- 2023-09-01 US US18/459,729 patent/US20250075233A1/en active Pending
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